AlfalfaGEDB | MS.gene60259

M. sativa cultivar XinJiangDaYe / MS.gene60259

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60259.t1	XP_004498649.1	100	84	0	0	1	84	51	134	4.80E-37	163.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60259.t1	Q8H7Y8	98.8	83	1	0	2	84	56	138	9.9e-39	160.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60259.t1	G7I6D5	100.0	84	0	0	1	84	51	134	3.5e-37	163.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene60259	0.84435	7.98E-59	-1.69E-46
MS.gene050290	MS.gene60259	0.819898	9.12E-53	-1.69E-46
MS.gene050916	MS.gene60259	0.858642	7.16E-63	-1.69E-46
MS.gene051330	MS.gene60259	0.819277	1.26E-52	-1.69E-46
MS.gene051746	MS.gene60259	0.800604	1.33E-48	-1.69E-46
MS.gene051903	MS.gene60259	0.862864	3.75E-64	-1.69E-46
MS.gene052947	MS.gene60259	0.842313	2.79E-58	-1.69E-46
MS.gene054937	MS.gene60259	0.848868	4.66E-60	-1.69E-46
MS.gene056075	MS.gene60259	0.857603	1.46E-62	-1.69E-46
MS.gene056631	MS.gene60259	0.833925	4.03E-56	-1.69E-46
MS.gene057094	MS.gene60259	0.817296	3.55E-52	-1.69E-46
MS.gene057705	MS.gene60259	0.860857	1.54E-63	-1.69E-46
MS.gene06001	MS.gene60259	0.861195	1.22E-63	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60259.t1	MTR_1g011750	100.000	84	0	0	1	84	51	134	1.70e-55	167
MS.gene60259.t1	MTR_1g011850	100.000	84	0	0	1	84	51	134	1.70e-55	167
MS.gene60259.t1	MTR_1g071040	92.857	84	6	0	1	84	51	134	3.40e-51	157
MS.gene60259.t1	MTR_1g114050	88.095	84	10	0	1	84	48	131	2.35e-48	149
MS.gene60259.t1	MTR_4g048490	83.582	67	11	0	18	84	39	105	2.60e-35	115
MS.gene60259.t1	MTR_7g108320	63.636	77	27	1	5	80	50	126	3.95e-28	99.0
MS.gene60259.t1	MTR_7g114060	63.636	77	27	1	5	80	47	123	8.17e-28	98.2
MS.gene60259.t1	MTR_7g114040	63.636	77	27	1	5	80	47	123	8.17e-28	98.2
MS.gene60259.t1	MTR_2g082220	63.291	79	28	1	5	82	44	122	2.11e-27	96.7
MS.gene60259.t1	MTR_2g082510	63.291	79	28	1	5	82	44	122	2.88e-27	96.3
MS.gene60259.t1	MTR_7g093910	62.338	77	28	1	5	80	50	126	4.12e-27	96.3
MS.gene60259.t1	MTR_7g113160	58.228	79	32	1	5	82	54	132	1.78e-25	92.4
MS.gene60259.t1	MTR_8g086640	60.494	81	31	1	5	84	52	132	2.22e-25	92.0
MS.gene60259.t1	MTR_4g071150	60.494	81	31	1	5	84	52	132	3.90e-25	91.7
MS.gene60259.t1	MTR_4g064967	59.259	81	32	1	5	84	52	132	4.77e-25	91.3
MS.gene60259.t1	MTR_4g063280	59.259	81	32	1	5	84	46	126	5.03e-25	91.3
MS.gene60259.t1	MTR_4g064005	59.259	81	32	1	5	84	52	132	5.94e-25	90.9
MS.gene60259.t1	MTR_2g096570	59.259	81	32	1	5	84	51	131	6.75e-25	90.9
MS.gene60259.t1	MTR_2g096610	59.259	81	32	1	5	84	50	130	7.14e-25	90.9
MS.gene60259.t1	MTR_4g063410	61.039	77	29	1	5	80	52	128	3.20e-24	89.4
MS.gene60259.t1	MTR_0653s0030	87.500	48	6	0	15	62	50	97	3.54e-23	86.7
MS.gene60259.t1	MTR_4g063300	62.687	67	24	1	5	70	17	83	2.34e-20	78.2
MS.gene60259.t1	MTR_7g027610	57.500	80	10	1	5	84	43	98	2.37e-20	78.2
MS.gene60259.t1	MTR_7g114050	51.899	79	26	3	5	82	49	116	1.13e-16	69.3
MS.gene60259.t1	MTR_7g427690	78.947	38	8	0	46	83	412	449	8.32e-13	62.0
MS.gene60259.t1	MTR_6g005150	43.750	80	35	3	5	82	54	125	5.83e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60259.t1	AT1G52740	96.386	83	3	0	2	84	52	134	6.95e-53	161
MS.gene60259.t1	AT3G54560	95.181	83	4	0	2	84	54	136	9.52e-53	160
MS.gene60259.t1	AT3G54560	95.181	83	4	0	2	84	54	136	9.52e-53	160
MS.gene60259.t1	AT2G38810	92.771	83	6	0	2	84	54	136	1.52e-51	158
MS.gene60259.t1	AT2G38810	92.771	83	6	0	2	84	54	136	1.52e-51	158
MS.gene60259.t1	AT2G38810	92.771	83	6	0	2	84	54	136	1.52e-51	158
MS.gene60259.t1	AT2G38810	92.771	83	6	0	2	84	54	136	1.52e-51	158
MS.gene60259.t1	AT4G13570	82.192	73	13	0	2	74	46	118	6.52e-39	125
MS.gene60259.t1	AT4G13570	82.192	73	13	0	2	74	52	124	2.84e-38	124
MS.gene60259.t1	AT1G54690	64.000	75	26	1	5	78	50	124	1.71e-27	97.4
MS.gene60259.t1	AT1G08880	64.000	75	26	1	5	78	50	124	1.73e-27	97.4
MS.gene60259.t1	AT4G27230	63.291	79	28	1	5	82	44	122	2.26e-27	96.7
MS.gene60259.t1	AT4G27230	63.291	79	28	1	5	82	44	122	2.26e-27	96.7
MS.gene60259.t1	AT1G51060	63.291	79	28	1	5	82	44	122	2.32e-27	96.7
MS.gene60259.t1	AT5G54640	63.291	79	28	1	5	82	44	122	2.62e-27	96.7
MS.gene60259.t1	AT3G20670	63.291	79	28	1	5	82	44	122	3.26e-27	96.3
MS.gene60259.t1	AT5G02560	62.963	81	29	1	5	84	52	132	4.17e-27	96.7
MS.gene60259.t1	AT5G27670	63.291	79	28	1	5	82	53	131	3.71e-26	94.4
MS.gene60259.t1	AT5G59870	63.291	79	28	1	5	82	52	130	5.36e-26	94.0
MS.gene60259.t1	AT5G02560	48.571	105	29	2	5	84	52	156	1.07e-21	83.6

Find 24 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGAACATTCATTCCTTTG+AGG	0.376752	1.1:-8857292	None:intergenic
CAATGGCACATGGAAGAGTT+GGG	0.442531	1.1:+8857049	None:intergenic
ACATGGAAGAGTTGGGGCTA+CGG	0.468116	1.1:+8857056	MS.gene60259:CDS
ACAATGGCACATGGAAGAGT+TGG	0.476378	1.1:+8857048	None:intergenic
GTATTTAACTGCAGAGGTGT+TGG	0.485770	1.1:+8857107	MS.gene60259:CDS
CTCATCAAAGGAACAATAGC+TGG	0.487626	1.1:+8857225	MS.gene60259:CDS
ATAAGAGACTTGTGGATATG+AGG	0.507095	1.1:-8857261	None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG	0.540859	1.1:+8857113	MS.gene60259:CDS
GCAGAGGTGTTGGAGTTGGC+TGG	0.558822	1.1:+8857117	MS.gene60259:CDS
CAATGCAAGCAAGGATCTGA+AGG	0.565081	1.1:+8857140	MS.gene60259:CDS
GTTGGCTGGCAATGCAAGCA+AGG	0.571379	1.1:+8857131	MS.gene60259:CDS
GAACTTGACACTCTCATCAA+AGG	0.571379	1.1:+8857213	MS.gene60259:CDS
ACTTGTTGATAAGAGACTTG+TGG	0.572936	1.1:-8857269	None:intergenic
TGTCAAGTTCTTCGTCTCCA+CGG	0.583078	1.1:-8857200	None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG	0.584334	1.1:-8857177	None:intergenic
TCTTATCAACAAGTCCTCAA+AGG	0.584732	1.1:+8857278	MS.gene60259:CDS
CATCTACAGCTTGCTATCCG+TGG	0.586408	1.1:+8857183	MS.gene60259:CDS
CCTCTGCAGTTAAATACTCT+AGG	0.598269	1.1:-8857101	None:intergenic
AAAGGAACAATAGCTGGTGG+TGG	0.608922	1.1:+8857231	MS.gene60259:CDS
CCTAGAGTATTTAACTGCAG+AGG	0.617705	1.1:+8857101	MS.gene60259:CDS
AAGTCAAGGACAATGGCACA+TGG	0.622887	1.1:+8857039	None:intergenic
AATGGCACATGGAAGAGTTG+GGG	0.645846	1.1:+8857050	None:intergenic
ATCAAAGGAACAATAGCTGG+TGG	0.661257	1.1:+8857228	MS.gene60259:CDS
AGTTAAATACTCTAGGATAG+CGG	0.703360	1.1:-8857094	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAAATACTCTAGGATAG+CGG	-	chr1.1:8857097-8857116	None:intergenic	30.0%
	ATAAGAGACTTGTGGATATG+AGG	-	chr1.1:8857264-8857283	None:intergenic	35.0%
	TCTTATCAACAAGTCCTCAA+AGG	+	chr1.1:8857278-8857297	MS.gene60259:CDS	35.0%
!	ACTTGTTGATAAGAGACTTG+TGG	-	chr1.1:8857272-8857291	None:intergenic	35.0%
	ATAGCAAGCTGTAGATGTCT+CGG	-	chr1.1:8857180-8857199	None:intergenic	40.0%
	ATCAAAGGAACAATAGCTGG+TGG	+	chr1.1:8857228-8857247	MS.gene60259:CDS	40.0%
	CCTCTGCAGTTAAATACTCT+AGG	-	chr1.1:8857104-8857123	None:intergenic	40.0%
	CTCATCAAAGGAACAATAGC+TGG	+	chr1.1:8857225-8857244	MS.gene60259:CDS	40.0%
	GAACTTGACACTCTCATCAA+AGG	+	chr1.1:8857213-8857232	MS.gene60259:CDS	40.0%
!	CCTAGAGTATTTAACTGCAG+AGG	+	chr1.1:8857101-8857120	MS.gene60259:CDS	40.0%
!	GTATTTAACTGCAGAGGTGT+TGG	+	chr1.1:8857107-8857126	MS.gene60259:CDS	40.0%
	AAAGGAACAATAGCTGGTGG+TGG	+	chr1.1:8857231-8857250	MS.gene60259:CDS	45.0%
	CAATGCAAGCAAGGATCTGA+AGG	+	chr1.1:8857140-8857159	MS.gene60259:CDS	45.0%
	TGTCAAGTTCTTCGTCTCCA+CGG	-	chr1.1:8857203-8857222	None:intergenic	45.0%
	ACATGGAAGAGTTGGGGCTA+CGG	+	chr1.1:8857056-8857075	MS.gene60259:CDS	50.0%
!!	AACTGCAGAGGTGTTGGAGT+TGG	+	chr1.1:8857113-8857132	MS.gene60259:CDS	50.0%
!!	CATCTACAGCTTGCTATCCG+TGG	+	chr1.1:8857183-8857202	MS.gene60259:CDS	50.0%
	GTTGGCTGGCAATGCAAGCA+AGG	+	chr1.1:8857131-8857150	MS.gene60259:CDS	55.0%
!!	GCAGAGGTGTTGGAGTTGGC+TGG	+	chr1.1:8857117-8857136	MS.gene60259:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	8857051	8857305	8857051	ID=MS.gene60259
chr1.1	mRNA	8857051	8857305	8857051	ID=MS.gene60259.t1;Parent=MS.gene60259
chr1.1	exon	8857051	8857305	8857051	ID=MS.gene60259.t1.exon1;Parent=MS.gene60259.t1
chr1.1	CDS	8857051	8857305	8857051	ID=cds.MS.gene60259.t1;Parent=MS.gene60259.t1

Gene Sequence	>MS.gene60259 ATGGCACATGGAAGAGTTGGGGCTACGGCTGCAGTTTATTCTGCCGCTATCCTAGAGTATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGACGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA
Protein sequence	>MS.gene60259.t1 MAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE