AlfalfaGEDB | MS.gene60578

M. sativa cultivar XinJiangDaYe / MS.gene60578

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030246
PFAM: PF00139.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60578.t1	XP_003599063.1	77.1	157	34	1	1	157	127	281	2.90E-59	238

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60578.t1	P16270	56.5	154	64	3	1	154	115	265	3.4e-40	166.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60578.t1	G7J1U2	77.1	157	34	1	1	157	127	281	2.1e-59	238.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene60578	0.84938	3.36E-60	-1.69E-46
MS.gene052011	MS.gene60578	0.804314	2.29E-49	-1.69E-46
MS.gene052223	MS.gene60578	0.806403	8.37E-50	-1.69E-46
MS.gene05240	MS.gene60578	0.812514	4.08E-51	-1.69E-46
MS.gene052702	MS.gene60578	0.824504	7.79E-54	-1.69E-46
MS.gene054277	MS.gene60578	0.808423	3.12E-50	-1.69E-46
MS.gene054742	MS.gene60578	0.803846	2.87E-49	-1.69E-46
MS.gene055601	MS.gene60578	0.819899	9.11E-53	-1.69E-46
MS.gene056330	MS.gene60578	0.818607	1.79E-52	-1.69E-46
MS.gene056688	MS.gene60578	0.808835	2.55E-50	-1.69E-46
MS.gene056690	MS.gene60578	0.838725	2.42E-57	-1.69E-46
MS.gene056691	MS.gene60578	0.824784	6.69E-54	-1.69E-46
MS.gene056697	MS.gene60578	0.809544	1.80E-50	-1.69E-46
MS.gene056899	MS.gene60578	0.832296	1.03E-55	-1.69E-46
MS.gene056920	MS.gene60578	0.815073	1.11E-51	-1.69E-46
MS.gene059043	MS.gene60578	0.818666	1.74E-52	-1.69E-46
MS.gene059402	MS.gene60578	0.828458	8.89E-55	-1.69E-46
MS.gene060087	MS.gene60578	0.848465	6.03E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60578.t1	MTR_3g027280	77.070	157	34	1	1	157	127	281	2.13e-81	241
MS.gene60578.t1	MTR_1g090957	77.070	157	34	1	1	157	115	269	2.21e-80	239
MS.gene60578.t1	MTR_1g090930	77.273	154	31	2	1	152	122	273	5.95e-77	231
MS.gene60578.t1	MTR_1g090937	72.549	153	40	2	1	153	120	270	3.18e-76	228
MS.gene60578.t1	MTR_3g054170	58.741	143	55	2	1	141	113	253	7.12e-50	160
MS.gene60578.t1	MTR_3g054180	57.241	145	56	3	1	141	117	259	3.30e-47	154
MS.gene60578.t1	MTR_1g090973	52.201	159	69	5	1	156	119	273	9.12e-46	150
MS.gene60578.t1	MTR_3g054200	55.556	144	58	3	1	141	113	253	2.57e-42	141
MS.gene60578.t1	MTR_0684s0020	52.288	153	68	3	1	151	118	267	5.54e-42	141
MS.gene60578.t1	MTR_0163s0020	52.288	153	68	3	1	151	119	268	5.56e-42	141
MS.gene60578.t1	MTR_3g047140	51.974	152	67	4	1	149	119	267	3.43e-38	131
MS.gene60578.t1	MTR_1g090960	45.783	166	56	5	1	157	104	244	7.81e-37	127
MS.gene60578.t1	MTR_1g090943	47.857	140	71	1	1	140	121	258	2.36e-35	124
MS.gene60578.t1	MTR_8g068040	40.506	158	70	9	2	141	109	260	2.32e-21	87.4
MS.gene60578.t1	MTR_8g068030	38.889	162	71	9	2	141	109	264	5.15e-21	86.7
MS.gene60578.t1	MTR_5g031160	35.714	154	83	6	2	141	135	286	6.83e-21	86.7
MS.gene60578.t1	MTR_8g068050	39.869	153	75	6	2	141	124	272	8.22e-21	88.2
MS.gene60578.t1	MTR_5g031140	38.312	154	79	7	2	141	134	285	1.47e-20	85.5
MS.gene60578.t1	MTR_8g023510	42.254	142	75	5	2	138	116	255	1.84e-20	85.1
MS.gene60578.t1	MTR_5g031120	40.909	154	75	9	2	141	127	278	2.43e-20	85.1
MS.gene60578.t1	MTR_3g463370	36.747	166	89	5	2	151	73	238	1.36e-19	82.4
MS.gene60578.t1	MTR_5g031100	36.601	153	82	6	2	141	127	277	8.53e-19	80.9
MS.gene60578.t1	MTR_8g067980	36.184	152	82	6	1	141	111	258	9.76e-19	80.5
MS.gene60578.t1	MTR_4g023810	38.462	143	79	6	2	140	115	252	4.87e-18	78.6
MS.gene60578.t1	MTR_8g067990	35.762	151	81	7	2	140	111	257	5.11e-17	75.9
MS.gene60578.t1	MTR_4g093080	36.842	152	82	6	2	141	109	258	3.83e-16	74.7
MS.gene60578.t1	MTR_5g031030	37.662	154	81	8	2	143	124	274	5.15e-16	73.2
MS.gene60578.t1	MTR_8g067960	35.948	153	82	7	2	143	94	241	1.45e-13	66.2
MS.gene60578.t1	MTR_1g014240	33.533	167	91	8	1	151	121	283	2.69e-13	66.6
MS.gene60578.t1	MTR_8g067720	34.667	150	86	6	2	141	109	256	5.19e-13	65.9
MS.gene60578.t1	MTR_4g093110	39.516	124	54	8	30	141	149	263	2.49e-12	63.9
MS.gene60578.t1	MTR_4g093140	38.710	124	55	8	30	141	149	263	1.74e-11	61.6
MS.gene60578.t1	MTR_4g093040	34.899	149	79	8	1	135	34	178	2.05e-11	61.2
MS.gene60578.t1	MTR_4g093040	34.899	149	79	8	1	135	116	260	2.17e-11	61.2
MS.gene60578.t1	MTR_4g056360	36.364	121	67	5	29	140	112	231	8.21e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60578.t1	AT5G10530	37.255	153	81	6	2	141	103	253	1.96e-16	75.9
MS.gene60578.t1	AT5G65600	34.043	141	81	4	1	129	119	259	3.07e-13	66.6

Find 40 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATGCATTAATTATCTTATA+TGG	0.217894	1.2:+69154046	None:intergenic
AATATGGTACCTCATAGATT+TGG	0.272536	1.2:+69154376	None:intergenic
CTATCTGGTGCTTCAGTAAC+TGG	0.316918	1.2:-69154152	MS.gene60578:CDS
AGACCAAACCTAACCGTGTT+AGG	0.331065	1.2:+69154173	None:intergenic
AATGCTTACAATCAATTTGT+TGG	0.337940	1.2:-69154434	MS.gene60578:CDS
CTCATAGATTTGGGATCATA+AGG	0.359980	1.2:+69154386	None:intergenic
AACAACACTCAATGTGTTAG+AGG	0.369181	1.2:+69154259	None:intergenic
AGTTGACGTTGGTTCCAATA+TGG	0.371704	1.2:+69154360	None:intergenic
ACGGTTAGGTTTGGTCTATC+TGG	0.383443	1.2:-69154167	MS.gene60578:CDS
TTTCTCTCAAGGTCATGAAA+TGG	0.388433	1.2:-69154331	MS.gene60578:CDS
AAATCTATGAGGTACCATAT+TGG	0.393883	1.2:-69154374	MS.gene60578:CDS
GATCATAAGGATTGGTATAA+AGG	0.401771	1.2:+69154399	None:intergenic
AACTCAGAAAGTGCACGTTT+TGG	0.408486	1.2:-69154473	MS.gene60578:CDS
CTCCCTAACACGGTTAGGTT+TGG	0.415201	1.2:-69154176	MS.gene60578:CDS
ATATGGTACCTCATAGATTT+GGG	0.415491	1.2:+69154377	None:intergenic
ATGGTAGAAAATTTGCCATT+GGG	0.420085	1.2:+69154227	None:intergenic
ATGAAATGGGAATGGGTGAC+TGG	0.427491	1.2:-69154317	MS.gene60578:CDS
CTGTACTCCCTAACACGGTT+AGG	0.428279	1.2:-69154181	MS.gene60578:CDS
TTCTCTCAAGGTCATGAAAT+GGG	0.430289	1.2:-69154330	MS.gene60578:CDS
AAACGTGCACTTTCTGAGTT+GGG	0.453097	1.2:+69154476	None:intergenic
AAAACGTGCACTTTCTGAGT+TGG	0.481047	1.2:+69154475	None:intergenic
CAAATCAACGATTTGAGCAA+TGG	0.492987	1.2:+69154208	None:intergenic
AATGGTAGAAAATTTGCCAT+TGG	0.495781	1.2:+69154226	None:intergenic
TCAAGGTCATGAAATGGGAA+TGG	0.510357	1.2:-69154325	MS.gene60578:CDS
AAGTGCTGAATGACCATGAA+TGG	0.510801	1.2:+69154114	None:intergenic
TGTGTTAGAGGGAGAGTCAT+AGG	0.513568	1.2:+69154271	None:intergenic
CAAGGTCATGAAATGGGAAT+GGG	0.518239	1.2:-69154324	MS.gene60578:CDS
CAGCACACGACATCCATTCA+TGG	0.520101	1.2:-69154127	MS.gene60578:CDS
CAACTCTTTAATTTCTCTCA+AGG	0.521126	1.2:-69154342	MS.gene60578:CDS
GACCAAACCTAACCGTGTTA+GGG	0.528956	1.2:+69154174	None:intergenic
AGATTTGGGATCATAAGGAT+TGG	0.548372	1.2:+69154391	None:intergenic
CTTATGATCCCAAATCTATG+AGG	0.558220	1.2:-69154385	MS.gene60578:CDS
GTTGTTGTCACTTACCCCAA+TGG	0.586471	1.2:-69154242	MS.gene60578:CDS
AGAAATTAAAGAGTTGACGT+TGG	0.590878	1.2:+69154349	None:intergenic
TGGTAGAAAATTTGCCATTG+GGG	0.595930	1.2:+69154228	None:intergenic
TATGGTTTGTAACTTGTCTG+TGG	0.599274	1.2:+69154064	None:intergenic
GGTGGAATCACAGTGTCTTG+TGG	0.603572	1.2:+69154497	None:intergenic
CGTGCACTTTCTGAGTTGGG+TGG	0.613896	1.2:+69154479	None:intergenic
ACAACACTCAATGTGTTAGA+GGG	0.619552	1.2:+69154260	None:intergenic
GAAAACTGTACTCCCTAACA+CGG	0.659450	1.2:-69154186	MS.gene60578:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGCTTACAATCAATTTGT+TGG	-	chr1.2:69154139-69154158	MS.gene60578:CDS	25.0%
	AATATGGTACCTCATAGATT+TGG	+	chr1.2:69154200-69154219	None:intergenic	30.0%
	AATGGTAGAAAATTTGCCAT+TGG	+	chr1.2:69154350-69154369	None:intergenic	30.0%
	AGAAATTAAAGAGTTGACGT+TGG	+	chr1.2:69154227-69154246	None:intergenic	30.0%
	ATATGGTACCTCATAGATTT+GGG	+	chr1.2:69154199-69154218	None:intergenic	30.0%
	ATGGTAGAAAATTTGCCATT+GGG	+	chr1.2:69154349-69154368	None:intergenic	30.0%
!	AAATCTATGAGGTACCATAT+TGG	-	chr1.2:69154199-69154218	MS.gene60578:CDS	30.0%
!	CAACTCTTTAATTTCTCTCA+AGG	-	chr1.2:69154231-69154250	MS.gene60578:CDS	30.0%
!	GATCATAAGGATTGGTATAA+AGG	+	chr1.2:69154177-69154196	None:intergenic	30.0%
	AACAACACTCAATGTGTTAG+AGG	+	chr1.2:69154317-69154336	None:intergenic	35.0%
	ACAACACTCAATGTGTTAGA+GGG	+	chr1.2:69154316-69154335	None:intergenic	35.0%
	CAAATCAACGATTTGAGCAA+TGG	+	chr1.2:69154368-69154387	None:intergenic	35.0%
	CTTATGATCCCAAATCTATG+AGG	-	chr1.2:69154188-69154207	MS.gene60578:CDS	35.0%
	TGGTAGAAAATTTGCCATTG+GGG	+	chr1.2:69154348-69154367	None:intergenic	35.0%
	TTCTCTCAAGGTCATGAAAT+GGG	-	chr1.2:69154243-69154262	MS.gene60578:CDS	35.0%
	TTTCTCTCAAGGTCATGAAA+TGG	-	chr1.2:69154242-69154261	MS.gene60578:CDS	35.0%
!	AGATTTGGGATCATAAGGAT+TGG	+	chr1.2:69154185-69154204	None:intergenic	35.0%
!	CTCATAGATTTGGGATCATA+AGG	+	chr1.2:69154190-69154209	None:intergenic	35.0%
!	TATGGTTTGTAACTTGTCTG+TGG	+	chr1.2:69154512-69154531	None:intergenic	35.0%
	AAAACGTGCACTTTCTGAGT+TGG	+	chr1.2:69154101-69154120	None:intergenic	40.0%
	AAACGTGCACTTTCTGAGTT+GGG	+	chr1.2:69154100-69154119	None:intergenic	40.0%
	CAAGGTCATGAAATGGGAAT+GGG	-	chr1.2:69154249-69154268	MS.gene60578:CDS	40.0%
	GAAAACTGTACTCCCTAACA+CGG	-	chr1.2:69154387-69154406	MS.gene60578:CDS	40.0%
	TCAAGGTCATGAAATGGGAA+TGG	-	chr1.2:69154248-69154267	MS.gene60578:CDS	40.0%
!!	AACTCAGAAAGTGCACGTTT+TGG	-	chr1.2:69154100-69154119	MS.gene60578:CDS	40.0%
!!	AAGTGCTGAATGACCATGAA+TGG	+	chr1.2:69154462-69154481	None:intergenic	40.0%
!!	AGTTGACGTTGGTTCCAATA+TGG	+	chr1.2:69154216-69154235	None:intergenic	40.0%
	AGACCAAACCTAACCGTGTT+AGG	+	chr1.2:69154403-69154422	None:intergenic	45.0%
	ATGAAATGGGAATGGGTGAC+TGG	-	chr1.2:69154256-69154275	MS.gene60578:CDS	45.0%
	CTATCTGGTGCTTCAGTAAC+TGG	-	chr1.2:69154421-69154440	MS.gene60578:CDS	45.0%
	GACCAAACCTAACCGTGTTA+GGG	+	chr1.2:69154402-69154421	None:intergenic	45.0%
	TGTGTTAGAGGGAGAGTCAT+AGG	+	chr1.2:69154305-69154324	None:intergenic	45.0%
!	ACGGTTAGGTTTGGTCTATC+TGG	-	chr1.2:69154406-69154425	MS.gene60578:CDS	45.0%
!	GTTGTTGTCACTTACCCCAA+TGG	-	chr1.2:69154331-69154350	MS.gene60578:CDS	45.0%
	CAGCACACGACATCCATTCA+TGG	-	chr1.2:69154446-69154465	MS.gene60578:CDS	50.0%
	CTCCCTAACACGGTTAGGTT+TGG	-	chr1.2:69154397-69154416	MS.gene60578:CDS	50.0%
	CTGTACTCCCTAACACGGTT+AGG	-	chr1.2:69154392-69154411	MS.gene60578:CDS	50.0%
	GGTGGAATCACAGTGTCTTG+TGG	+	chr1.2:69154079-69154098	None:intergenic	50.0%
!	CGTGCACTTTCTGAGTTGGG+TGG	+	chr1.2:69154097-69154116	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	69154061	69154534	69154061	ID=MS.gene60578
chr1.2	mRNA	69154061	69154534	69154061	ID=MS.gene60578.t1;Parent=MS.gene60578
chr1.2	exon	69154061	69154534	69154061	ID=MS.gene60578.t1.exon1;Parent=MS.gene60578.t1
chr1.2	CDS	69154061	69154534	69154061	ID=cds.MS.gene60578.t1;Parent=MS.gene60578.t1

Gene Sequence

>MS.gene60578

ATGTTCTTTATTGCACCACAAGACACTGTGATTCCACCCAACTCAGAAAGTGCACGTTTTGGAGTAGTTGATTCAAAAAATGCTTACAATCAATTTGTTGGTGTAGAGTTTGACCTTTATACCAATCCTTATGATCCCAAATCTATGAGGTACCATATTGGAACCAACGTCAACTCTTTAATTTCTCTCAAGGTCATGAAATGGGAATGGGTGACTGGATCATTGAATGAAGTAAGTATAGCCTATGACTCTCCCTCTAACACATTGAGTGTTGTTGTCACTTACCCCAATGGCAAATTTTCTACCATTGCTCAAATCGTTGATTTGAAAACTGTACTCCCTAACACGGTTAGGTTTGGTCTATCTGGTGCTTCAGTAACTGGTACAGCACACGACATCCATTCATGGTCATTCAGCACTTCAAACTTGAAAACAACTATAAGCAGCGCCACAGACAAGTTACAAACCATATAA

Protein sequence

>MS.gene60578.t1

MFFIAPQDTVIPPNSESARFGVVDSKNAYNQFVGVEFDLYTNPYDPKSMRYHIGTNVNSLISLKVMKWEWVTGSLNEVSIAYDSPSNTLSVVVTYPNGKFSTIAQIVDLKTVLPNTVRFGLSGASVTGTAHDIHSWSFSTSNLKTTISSATDKLQTI