Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60825.t1 | KEH43435.1 | 99.4 | 162 | 1 | 0 | 1 | 162 | 1 | 162 | 4.80E-57 | 230.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60825.t1 | A0A072VZG1 | 99.4 | 162 | 1 | 0 | 1 | 162 | 1 | 162 | 3.4e-57 | 230.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene60825 | 0.804239 | 2.38E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene60825 | 0.814762 | 1.31E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene60825 | 0.825212 | 5.30E-54 | -1.69E-46 |
| MS.gene050237 | MS.gene60825 | 0.821189 | 4.61E-53 | -1.69E-46 |
| MS.gene050807 | MS.gene60825 | 0.818975 | 1.48E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene60825 | 0.838601 | 2.61E-57 | -1.69E-46 |
| MS.gene051102 | MS.gene60825 | 0.800105 | 1.68E-48 | -1.69E-46 |
| MS.gene051328 | MS.gene60825 | 0.804851 | 1.77E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene60825 | 0.81079 | 9.67E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene60825 | 0.805504 | 1.29E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene60825 | 0.802053 | 6.73E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene60825 | 0.816649 | 4.96E-52 | -1.69E-46 |
| MS.gene052335 | MS.gene60825 | 0.822179 | 2.72E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene60825 | 0.822606 | 2.17E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene60825 | 0.80364 | 3.16E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene60825 | 0.820774 | 5.74E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene60825 | 0.819218 | 1.30E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene60825 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene60825 | 0.856113 | 4.01E-62 | -1.69E-46 |
| MS.gene05462 | MS.gene60825 | 0.814578 | 1.43E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene60825 | 0.835054 | 2.10E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene60825 | 0.824554 | 7.58E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene60825 | 0.84508 | 5.08E-59 | -1.69E-46 |
| MS.gene055469 | MS.gene60825 | 0.849658 | 2.81E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene60825 | -0.8019 | 7.24E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene60825 | -0.810379 | 1.19E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene60825 | 0.800202 | 1.61E-48 | -1.69E-46 |
| MS.gene056308 | MS.gene60825 | 0.805747 | 1.15E-49 | -1.69E-46 |
| MS.gene057446 | MS.gene60825 | 0.801815 | 7.54E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene60825 | 0.805002 | 1.65E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene60825 | 0.808093 | 3.67E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene60825 | 0.800417 | 1.46E-48 | -1.69E-46 |
| MS.gene061006 | MS.gene60825 | 0.831869 | 1.31E-55 | -1.69E-46 |
| MS.gene061100 | MS.gene60825 | 0.831826 | 1.34E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60825.t1 | MTR_1g492930 | 99.383 | 162 | 1 | 0 | 1 | 162 | 1 | 162 | 3.98e-113 | 318 |
| MS.gene60825.t1 | MTR_7g104020 | 60.127 | 158 | 60 | 1 | 4 | 161 | 14 | 168 | 3.31e-51 | 161 |
| MS.gene60825.t1 | MTR_7g104020 | 58.385 | 161 | 61 | 2 | 4 | 161 | 14 | 171 | 9.29e-50 | 158 |
| MS.gene60825.t1 | MTR_2g085025 | 56.129 | 155 | 67 | 1 | 4 | 158 | 20 | 173 | 3.24e-43 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60825.t1 | AT3G52900 | 60.526 | 152 | 60 | 0 | 7 | 158 | 12 | 163 | 3.17e-59 | 181 |
| MS.gene60825.t1 | AT2G36410 | 56.863 | 153 | 63 | 1 | 6 | 158 | 43 | 192 | 9.56e-54 | 169 |
| MS.gene60825.t1 | AT3G52920 | 56.329 | 158 | 66 | 1 | 2 | 159 | 10 | 164 | 1.75e-53 | 167 |
| MS.gene60825.t1 | AT2G36410 | 56.410 | 156 | 62 | 2 | 6 | 158 | 43 | 195 | 3.01e-52 | 165 |
| MS.gene60825.t1 | AT2G27740 | 54.658 | 161 | 71 | 2 | 1 | 161 | 16 | 174 | 3.87e-52 | 164 |
| MS.gene60825.t1 | AT3G52920 | 55.901 | 161 | 65 | 2 | 2 | 159 | 10 | 167 | 8.21e-52 | 163 |
| MS.gene60825.t1 | AT3G09980 | 62.179 | 156 | 57 | 2 | 5 | 158 | 22 | 177 | 4.28e-50 | 159 |
| MS.gene60825.t1 | AT2G36355 | 56.494 | 154 | 62 | 2 | 6 | 158 | 5 | 154 | 1.68e-49 | 157 |
| MS.gene60825.t1 | AT5G03660 | 59.459 | 148 | 59 | 1 | 10 | 156 | 22 | 169 | 1.39e-41 | 137 |
| MS.gene60825.t1 | AT2G36355 | 68.605 | 86 | 27 | 0 | 73 | 158 | 27 | 112 | 1.06e-34 | 117 |
| MS.gene60825.t1 | AT5G03660 | 58.462 | 130 | 53 | 1 | 10 | 138 | 22 | 151 | 1.33e-33 | 116 |
| MS.gene60825.t1 | AT2G36355 | 68.675 | 83 | 26 | 0 | 76 | 158 | 1 | 83 | 3.65e-33 | 113 |
| MS.gene60825.t1 | AT2G36410 | 54.369 | 103 | 44 | 1 | 6 | 108 | 43 | 142 | 1.01e-30 | 108 |
| MS.gene60825.t1 | AT2G27740 | 50.926 | 108 | 52 | 1 | 1 | 108 | 16 | 122 | 1.49e-29 | 105 |
| MS.gene60825.t1 | AT5G03660 | 50.676 | 148 | 50 | 2 | 10 | 156 | 22 | 147 | 1.40e-27 | 100 |
| MS.gene60825.t1 | AT2G36355 | 50.000 | 104 | 47 | 2 | 6 | 108 | 5 | 104 | 6.32e-25 | 93.6 |
Find 31 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGAGGATGTTTCAAAAT+TGG | 0.314571 | 1.4:-72838877 | MS.gene60825:CDS |
| CAAGGCGTTTGGCTCACATT+TGG | 0.338322 | 1.4:-72838755 | MS.gene60825:CDS |
| TGGACAGAACTACCAGAAAA+AGG | 0.350574 | 1.4:-72838319 | MS.gene60825:intron |
| ATGCAGAAAATGAAGAATCT+TGG | 0.366016 | 1.4:-72838942 | MS.gene60825:CDS |
| AAGAATAAGGATGAAGAAAC+TGG | 0.374263 | 1.4:-72837930 | MS.gene60825:CDS |
| CTTAGCTTGTAATGTAGAAA+TGG | 0.384471 | 1.4:+72838854 | None:intergenic |
| TTTCAGTGACACAGACTCTA+TGG | 0.394269 | 1.4:+72837892 | None:intergenic |
| AAGGCGTTTGGCTCACATTT+GGG | 0.402285 | 1.4:-72838754 | MS.gene60825:CDS |
| ATTGCGACTTCCTTCCTCAT+AGG | 0.404710 | 1.4:+72838387 | None:intergenic |
| ATGAAGAATCTTGGGAGTAT+TGG | 0.415811 | 1.4:-72838933 | MS.gene60825:CDS |
| TGCAGAAAATGAAGAATCTT+GGG | 0.429002 | 1.4:-72838941 | MS.gene60825:CDS |
| GAACAAAGAGAAGAATCATT+TGG | 0.441757 | 1.4:-72838093 | MS.gene60825:CDS |
| GAAAGAGTACAAAGAAGCCT+TGG | 0.456836 | 1.4:-72838132 | MS.gene60825:CDS |
| TGGCTGAGAGTGAAAGAATA+AGG | 0.460290 | 1.4:-72837943 | MS.gene60825:CDS |
| TGAAGAAGAGACAAGGCGTT+TGG | 0.480572 | 1.4:-72838766 | MS.gene60825:CDS |
| AACAAAGACGTAAAGCAACT+TGG | 0.481616 | 1.4:-72838339 | MS.gene60825:CDS |
| AATCTAGGAGCTTGAAGTGA+TGG | 0.511396 | 1.4:-72838415 | MS.gene60825:intron |
| CATTTCTACATTACAAGCTA+AGG | 0.513379 | 1.4:-72838853 | MS.gene60825:CDS |
| GAGAGAGTTGAACTTCAACT+TGG | 0.532697 | 1.4:-72838795 | MS.gene60825:CDS |
| AACAGAAGAGTGTGAAGATG+AGG | 0.569715 | 1.4:-72838892 | MS.gene60825:CDS |
| CTTGGGAGTATTGGAAGCAG+TGG | 0.571069 | 1.4:-72838924 | MS.gene60825:CDS |
| CTAAGGAAGAAGAGATTGAG+AGG | 0.578478 | 1.4:-72838836 | MS.gene60825:CDS |
| AAGTGATGGAAGATCCTATG+AGG | 0.585128 | 1.4:-72838401 | MS.gene60825:CDS |
| GATGGAAGATCCTATGAGGA+AGG | 0.586096 | 1.4:-72838397 | MS.gene60825:CDS |
| GGTGCGTAAGAAAATTGACT+TGG | 0.609025 | 1.4:-72838364 | MS.gene60825:CDS |
| TTGGGAGTATTGGAAGCAGT+GGG | 0.627893 | 1.4:-72838923 | MS.gene60825:CDS |
| GGAGTATTGGAAGCAGTGGG+AGG | 0.639884 | 1.4:-72838920 | MS.gene60825:CDS |
| TATGAGGAAGGAAGTCGCAA+TGG | 0.648915 | 1.4:-72838385 | MS.gene60825:CDS |
| GACGTGCTGAAGAAGAGACA+AGG | 0.676772 | 1.4:-72838773 | MS.gene60825:CDS |
| TTTGGTAGCGACATTAACTG+AGG | 0.691001 | 1.4:-72838075 | MS.gene60825:intron |
| AATAAGGATGAAGAAACTGG+AGG | 0.697364 | 1.4:-72837927 | MS.gene60825:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTCAACAGAAAATAATA+TGG | + | chr1.4:72838543-72838562 | None:intergenic | 15.0% |
| !! | TTATTGAAATTAAATTCTAC+TGG | - | chr1.4:72838349-72838368 | MS.gene60825:CDS | 15.0% |
| !!! | ACCTTTAAAATTTACTTAAT+AGG | - | chr1.4:72838672-72838691 | MS.gene60825:intron | 15.0% |
| !!! | ATTTTGTCATATAATAATCT+AGG | - | chr1.4:72838398-72838417 | MS.gene60825:CDS | 15.0% |
| !!! | TCCTATTAAGTAAATTTTAA+AGG | + | chr1.4:72838676-72838695 | None:intergenic | 15.0% |
| !!! | TTATGTTTATTTTCTAAACA+CGG | + | chr1.4:72838109-72838128 | None:intergenic | 15.0% |
| !!! | TTTATTTTATTAATAGATGC+TGG | - | chr1.4:72838865-72838884 | MS.gene60825:CDS | 15.0% |
| !! | AAAATAAAAAATTGCAACCA+TGG | + | chr1.4:72838853-72838872 | None:intergenic | 20.0% |
| !!! | ATTGGATGATATTTTAAGTT+CGG | + | chr1.4:72838237-72838256 | None:intergenic | 20.0% |
| ! | AATATTGTTACCATGGTAAT+TGG | + | chr1.4:72838820-72838839 | None:intergenic | 25.0% |
| ! | CAAATTTACAAGACAAATCA+AGG | + | chr1.4:72838602-72838621 | None:intergenic | 25.0% |
| ! | GAGATAAAATATTGTTACCA+TGG | + | chr1.4:72838827-72838846 | None:intergenic | 25.0% |
| !! | ACATTTTGTTCAAGAAATGA+TGG | + | chr1.4:72838272-72838291 | None:intergenic | 25.0% |
| !! | CAATATTTTATCTCTTTCCA+TGG | - | chr1.4:72838833-72838852 | MS.gene60825:CDS | 25.0% |
| !!! | CTTGATTTGTCTTGTAAATT+TGG | - | chr1.4:72838600-72838619 | MS.gene60825:intron | 25.0% |
| AAGAATAAGGATGAAGAAAC+TGG | - | chr1.4:72838898-72838917 | MS.gene60825:CDS | 30.0% | |
| AGATGAGGATGTTTCAAAAT+TGG | - | chr1.4:72837951-72837970 | MS.gene60825:intron | 30.0% | |
| ATCAGACTTAAACTCAAATC+TGG | + | chr1.4:72838211-72838230 | None:intergenic | 30.0% | |
| ATGCAGAAAATGAAGAATCT+TGG | - | chr1.4:72837886-72837905 | MS.gene60825:CDS | 30.0% | |
| CATTTCTACATTACAAGCTA+AGG | - | chr1.4:72837975-72837994 | MS.gene60825:intron | 30.0% | |
| CTTTCTATTGTCAACAACAT+GGG | + | chr1.4:72838323-72838342 | None:intergenic | 30.0% | |
| GAACAAAGAGAAGAATCATT+TGG | - | chr1.4:72838735-72838754 | MS.gene60825:intron | 30.0% | |
| TGCAGAAAATGAAGAATCTT+GGG | - | chr1.4:72837887-72837906 | MS.gene60825:CDS | 30.0% | |
| !! | CTTAGCTTGTAATGTAGAAA+TGG | + | chr1.4:72837977-72837996 | None:intergenic | 30.0% |
| AACAAAGACGTAAAGCAACT+TGG | - | chr1.4:72838489-72838508 | MS.gene60825:intron | 35.0% | |
| AATAAGGATGAAGAAACTGG+AGG | - | chr1.4:72838901-72838920 | MS.gene60825:CDS | 35.0% | |
| ACTTAAACTCAAATCTGGAC+TGG | + | chr1.4:72838206-72838225 | None:intergenic | 35.0% | |
| AGAGATTGAGAGGAAAAAGA+TGG | - | chr1.4:72838002-72838021 | MS.gene60825:intron | 35.0% | |
| GCTTTCTATTGTCAACAACA+TGG | + | chr1.4:72838324-72838343 | None:intergenic | 35.0% | |
| ! | ATGAAGAATCTTGGGAGTAT+TGG | - | chr1.4:72837895-72837914 | MS.gene60825:CDS | 35.0% |
| ! | CTTTTCATTGAAAGCTTCCA+AGG | + | chr1.4:72838716-72838735 | None:intergenic | 35.0% |
| AATCTAGGAGCTTGAAGTGA+TGG | - | chr1.4:72838413-72838432 | MS.gene60825:intron | 40.0% | |
| CATAAGTGACCATCTCTCTT+AGG | - | chr1.4:72838124-72838143 | MS.gene60825:CDS | 40.0% | |
| CATGGATTTCCTAAGAGAGA+TGG | + | chr1.4:72838136-72838155 | None:intergenic | 40.0% | |
| CTAAGGAAGAAGAGATTGAG+AGG | - | chr1.4:72837992-72838011 | MS.gene60825:intron | 40.0% | |
| GACTTCGTTTCCAATTACCA+TGG | - | chr1.4:72838807-72838826 | MS.gene60825:CDS | 40.0% | |
| GAGAGAGTTGAACTTCAACT+TGG | - | chr1.4:72838033-72838052 | MS.gene60825:intron | 40.0% | |
| GATTGAGAGGAAAAAGATGG+AGG | - | chr1.4:72838005-72838024 | MS.gene60825:intron | 40.0% | |
| GGTGCGTAAGAAAATTGACT+TGG | - | chr1.4:72838464-72838483 | MS.gene60825:intron | 40.0% | |
| TGATGGTCAGCAAACTAGAT+TGG | + | chr1.4:72838255-72838274 | None:intergenic | 40.0% | |
| TGGACAGAACTACCAGAAAA+AGG | - | chr1.4:72838509-72838528 | MS.gene60825:intron | 40.0% | |
| TGGCTGAGAGTGAAAGAATA+AGG | - | chr1.4:72838885-72838904 | MS.gene60825:CDS | 40.0% | |
| TTTCAGTGACACAGACTCTA+TGG | + | chr1.4:72838939-72838958 | None:intergenic | 40.0% | |
| ! | AACAGAAGAGTGTGAAGATG+AGG | - | chr1.4:72837936-72837955 | MS.gene60825:CDS | 40.0% |
| ! | AAGTGATGGAAGATCCTATG+AGG | - | chr1.4:72838427-72838446 | MS.gene60825:intron | 40.0% |
| ! | GAAAGAGTACAAAGAAGCCT+TGG | - | chr1.4:72838696-72838715 | MS.gene60825:intron | 40.0% |
| !! | TTTGGTAGCGACATTAACTG+AGG | - | chr1.4:72838753-72838772 | MS.gene60825:CDS | 40.0% |
| ATTGCGACTTCCTTCCTCAT+AGG | + | chr1.4:72838444-72838463 | None:intergenic | 45.0% | |
| GATGGAAGATCCTATGAGGA+AGG | - | chr1.4:72838431-72838450 | MS.gene60825:intron | 45.0% | |
| TATGAGGAAGGAAGTCGCAA+TGG | - | chr1.4:72838443-72838462 | MS.gene60825:intron | 45.0% | |
| ! | AAGGCGTTTGGCTCACATTT+GGG | - | chr1.4:72838074-72838093 | MS.gene60825:intron | 45.0% |
| ! | ATGGCTGTCGTACCTTTTTC+TGG | + | chr1.4:72838524-72838543 | None:intergenic | 45.0% |
| ! | TGAAGAAGAGACAAGGCGTT+TGG | - | chr1.4:72838062-72838081 | MS.gene60825:intron | 45.0% |
| !! | TTGGGAGTATTGGAAGCAGT+GGG | - | chr1.4:72837905-72837924 | MS.gene60825:CDS | 45.0% |
| AGGAAATCCATGCAGTCACG+CGG | - | chr1.4:72838144-72838163 | MS.gene60825:intron | 50.0% | |
| GACGTGCTGAAGAAGAGACA+AGG | - | chr1.4:72838055-72838074 | MS.gene60825:intron | 50.0% | |
| ! | CAAGGCGTTTGGCTCACATT+TGG | - | chr1.4:72838073-72838092 | MS.gene60825:intron | 50.0% |
| !! | CTTGGGAGTATTGGAAGCAG+TGG | - | chr1.4:72837904-72837923 | MS.gene60825:CDS | 50.0% |
| TTGTTGACCGCGTGACTGCA+TGG | + | chr1.4:72838154-72838173 | None:intergenic | 55.0% | |
| !! | GGAGTATTGGAAGCAGTGGG+AGG | - | chr1.4:72837908-72837927 | MS.gene60825:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 72837886 | 72838964 | 72837886 | ID=MS.gene60825 |
| chr1.4 | mRNA | 72837886 | 72838964 | 72837886 | ID=MS.gene60825.t1;Parent=MS.gene60825 |
| chr1.4 | exon | 72838752 | 72838964 | 72838752 | ID=MS.gene60825.t1.exon1;Parent=MS.gene60825.t1 |
| chr1.4 | CDS | 72838752 | 72838964 | 72838752 | ID=cds.MS.gene60825.t1;Parent=MS.gene60825.t1 |
| chr1.4 | exon | 72838320 | 72838430 | 72838320 | ID=MS.gene60825.t1.exon2;Parent=MS.gene60825.t1 |
| chr1.4 | CDS | 72838320 | 72838430 | 72838320 | ID=cds.MS.gene60825.t1;Parent=MS.gene60825.t1 |
| chr1.4 | exon | 72838076 | 72838156 | 72838076 | ID=MS.gene60825.t1.exon3;Parent=MS.gene60825.t1 |
| chr1.4 | CDS | 72838076 | 72838156 | 72838076 | ID=cds.MS.gene60825.t1;Parent=MS.gene60825.t1 |
| chr1.4 | exon | 72837886 | 72837969 | 72837886 | ID=MS.gene60825.t1.exon4;Parent=MS.gene60825.t1 |
| chr1.4 | CDS | 72837886 | 72837969 | 72837886 | ID=cds.MS.gene60825.t1;Parent=MS.gene60825.t1 |
| Gene Sequence |
| Protein sequence |
