AlfalfaGEDB | MS.gene61207

M. sativa cultivar XinJiangDaYe / MS.gene61207

KEGG: -
GO: -
PFAM: PF03168.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61207.t1	AES68023.2	98.5	132	2	0	1	132	1	132	4.90E-66	260.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61207.t1	G7II42	98.5	132	2	0	1	132	1	132	3.5e-66	260.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene61207	0.803753	3.00E-49	-1.69E-46
MS.gene050815	MS.gene61207	0.814573	1.44E-51	-1.69E-46
MS.gene05288	MS.gene61207	0.831654	1.48E-55	-1.69E-46
MS.gene05289	MS.gene61207	0.810566	1.08E-50	-1.69E-46
MS.gene053008	MS.gene61207	0.816063	6.71E-52	-1.69E-46
MS.gene054713	MS.gene61207	0.835411	1.70E-56	-1.69E-46
MS.gene054775	MS.gene61207	0.810427	1.16E-50	-1.69E-46
MS.gene054801	MS.gene61207	0.810361	1.20E-50	-1.69E-46
MS.gene055608	MS.gene61207	0.852331	4.95E-61	-1.69E-46
MS.gene055926	MS.gene61207	0.804391	2.21E-49	-1.69E-46
MS.gene057693	MS.gene61207	0.80877	2.63E-50	-1.69E-46
MS.gene05979	MS.gene61207	0.800278	1.55E-48	-1.69E-46
MS.gene059842	MS.gene61207	0.814027	1.90E-51	-1.69E-46
MS.gene060322	MS.gene61207	0.804785	1.83E-49	-1.69E-46
MS.gene060429	MS.gene61207	0.80053	1.38E-48	-1.69E-46
MS.gene060802	MS.gene61207	0.807385	5.19E-50	-1.69E-46
MS.gene060803	MS.gene61207	0.820285	7.44E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61207.t1	MTR_2g102080	94.286	140	8	0	1	140	1	140	2.37e-92	266
MS.gene61207.t1	MTR_4g012450	36.154	130	82	1	1	130	34	162	1.38e-27	101
MS.gene61207.t1	MTR_6g086455	36.029	136	82	2	1	135	78	209	1.52e-23	92.4

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61207.t1	AT1G52330	54.135	133	61	1	133	59	191	3.64e-51	162
MS.gene61207.t1	AT1G52330	53.333	135	63	1	135	59	193	3.72e-51	162
MS.gene61207.t1	AT4G13270	43.182	132	75	1	132	60	191	1.32e-36	125

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAGTTAGGTTATAGTTTA+TGG	0.131918	2.1:+3235698	MS.gene61207:intron
AAGAAAATGATTTACCTTAA+TGG	0.212531	2.1:-3235610	None:intergenic
AGAAAATGATTTACCTTAAT+GGG	0.264332	2.1:-3235609	None:intergenic
TAGTCAAGTTAGGGGCAAAA+TGG	0.292543	2.1:+3235566	MS.gene61207:CDS
TTGATACGGTTAGTCAAGTT+AGG	0.332171	2.1:+3235556	MS.gene61207:CDS
GTTGAAGCTGAAGAAGATTA+AGG	0.365406	2.1:+3235266	MS.gene61207:CDS
AGTCAAGTTAGGGGCAAAAT+GGG	0.396713	2.1:+3235567	MS.gene61207:CDS
GAATGGGCACGTGAGGGCTA+TGG	0.456284	2.1:+3235434	MS.gene61207:CDS
TGATACGGTTAGTCAAGTTA+GGG	0.457996	2.1:+3235557	MS.gene61207:CDS
GGCTATGGGATCTTCATTTG+TGG	0.458085	2.1:+3235449	MS.gene61207:CDS
TCCAACAACAACACTATCTC+AGG	0.473538	2.1:-3235507	None:intergenic
GGCACGTGACTTCGAAGAAT+GGG	0.476369	2.1:+3235418	MS.gene61207:CDS
ACCTGAGATAGTGTTGTTGT+TGG	0.476726	2.1:+3235506	MS.gene61207:CDS
AATGGGCACGTGAGGGCTAT+GGG	0.477387	2.1:+3235435	MS.gene61207:CDS
AAATGTTGACGTTTATTCCA+TGG	0.481008	2.1:+3235347	MS.gene61207:CDS
GGGCACGTGACTTCGAAGAA+TGG	0.502859	2.1:+3235417	MS.gene61207:CDS
GTTGTTGGAGGACCTTGCTA+AGG	0.507070	2.1:+3235521	MS.gene61207:CDS
GATGCAGAATTCAGTGGTAT+TGG	0.514947	2.1:+3235477	MS.gene61207:CDS
GACGTTTATTCCATGGACTT+TGG	0.517964	2.1:+3235354	MS.gene61207:CDS
AACCGTACGATCTTGAGGTA+TGG	0.521084	2.1:-3235246	None:intergenic
TCTTATAGAGGGAAACCACT+TGG	0.536597	2.1:+3235396	MS.gene61207:CDS
GGGTATGATACCCTTTGATA+CGG	0.543076	2.1:+3235542	MS.gene61207:CDS
TGAGATAGTGTTGTTGTTGG+AGG	0.545985	2.1:+3235509	MS.gene61207:CDS
CTTCGAAGAATGGGCACGTG+AGG	0.549210	2.1:+3235427	MS.gene61207:CDS
TTGTTGGAGGACCTTGCTAA+GGG	0.551961	2.1:+3235522	MS.gene61207:CDS
CACTCTCAACGTGAAGAGCA+TGG	0.555106	2.1:-3235322	None:intergenic
GTCAACAGCACCAAAGTCCA+TGG	0.556982	2.1:-3235364	None:intergenic
AACTTGACTAACCGTATCAA+AGG	0.559559	2.1:-3235553	None:intergenic
ACTTGACTAACCGTATCAAA+GGG	0.561858	2.1:-3235552	None:intergenic
ATCCATACCTCAAGATCGTA+CGG	0.564124	2.1:+3235244	MS.gene61207:CDS
GCTTCAACCGTACGATCTTG+AGG	0.564157	2.1:-3235251	None:intergenic
ATCTTGAGGTATGGATCTGA+CGG	0.564383	2.1:-3235237	None:intergenic
CATGCTCTTCACGTTGAGAG+TGG	0.566163	2.1:+3235323	MS.gene61207:CDS
GACGTGGCGGTATCTTATAG+AGG	0.568477	2.1:+3235384	MS.gene61207:CDS
TTCGAAGTCACGTGCCCAAG+TGG	0.585354	2.1:-3235411	None:intergenic
CTTATAGAGGGAAACCACTT+GGG	0.598189	2.1:+3235397	MS.gene61207:CDS
TTCGAAGAATGGGCACGTGA+GGG	0.610158	2.1:+3235428	MS.gene61207:CDS
GAAGAGCATGGAGATATCTA+CGG	0.612242	2.1:-3235310	None:intergenic
GATACGGTTAGTCAAGTTAG+GGG	0.618576	2.1:+3235558	MS.gene61207:CDS
GGACTTTGGTGCTGTTGACG+TGG	0.632167	2.1:+3235368	MS.gene61207:CDS
AGGGTATCATACCCTTAGCA+AGG	0.651835	2.1:-3235533	None:intergenic
GAGATATCTACGGTGATATG+CGG	0.652943	2.1:-3235300	None:intergenic
ACGTGGCGGTATCTTATAGA+GGG	0.653035	2.1:+3235385	MS.gene61207:CDS
GTCAAGTTAGGGGCAAAATG+GGG	0.655267	2.1:+3235568	MS.gene61207:CDS
TGACGGATAGAAAATATACA+TGG	0.674941	2.1:-3235220	None:intergenic
CTTTGGTGCTGTTGACGTGG+CGG	0.678358	2.1:+3235371	MS.gene61207:CDS
GATGCTGATGCAGAATTCAG+TGG	0.701186	2.1:+3235471	MS.gene61207:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTAATAATTTGAGTT+AGG	+	chr2.1:3235685-3235704	MS.gene61207:intron	10.0%
!!	AAGAAAATGATTTACCTTAA+TGG	-	chr2.1:3235613-3235632	None:intergenic	20.0%
!!	ACATATGATTATGAATAACT+TGG	-	chr2.1:3235640-3235659	None:intergenic	20.0%
!!	AGAAAATGATTTACCTTAAT+GGG	-	chr2.1:3235612-3235631	None:intergenic	20.0%
!	TTGAGTTAGGTTATAGTTTA+TGG	+	chr2.1:3235698-3235717	MS.gene61207:intron	25.0%
!!	ATTCTTTTACTTTCCCATTA+AGG	+	chr2.1:3235596-3235615	MS.gene61207:CDS	25.0%
	AAATGTTGACGTTTATTCCA+TGG	+	chr2.1:3235347-3235366	MS.gene61207:CDS	30.0%
	AACTTGACTAACCGTATCAA+AGG	-	chr2.1:3235556-3235575	None:intergenic	35.0%
	ACTTGACTAACCGTATCAAA+GGG	-	chr2.1:3235555-3235574	None:intergenic	35.0%
	GTTGAAGCTGAAGAAGATTA+AGG	+	chr2.1:3235266-3235285	MS.gene61207:CDS	35.0%
!	TGATACGGTTAGTCAAGTTA+GGG	+	chr2.1:3235557-3235576	MS.gene61207:CDS	35.0%
!	TTGATACGGTTAGTCAAGTT+AGG	+	chr2.1:3235556-3235575	MS.gene61207:CDS	35.0%
	AGTCAAGTTAGGGGCAAAAT+GGG	+	chr2.1:3235567-3235586	MS.gene61207:CDS	40.0%
	ATCCATACCTCAAGATCGTA+CGG	+	chr2.1:3235244-3235263	MS.gene61207:CDS	40.0%
	ATCTTGAGGTATGGATCTGA+CGG	-	chr2.1:3235240-3235259	None:intergenic	40.0%
	CTTATAGAGGGAAACCACTT+GGG	+	chr2.1:3235397-3235416	MS.gene61207:CDS	40.0%
	GACGTTTATTCCATGGACTT+TGG	+	chr2.1:3235354-3235373	MS.gene61207:CDS	40.0%
	GAGATATCTACGGTGATATG+CGG	-	chr2.1:3235303-3235322	None:intergenic	40.0%
	GATGCAGAATTCAGTGGTAT+TGG	+	chr2.1:3235477-3235496	MS.gene61207:CDS	40.0%
	GGGTATGATACCCTTTGATA+CGG	+	chr2.1:3235542-3235561	MS.gene61207:CDS	40.0%
	TAGTCAAGTTAGGGGCAAAA+TGG	+	chr2.1:3235566-3235585	MS.gene61207:CDS	40.0%
	TCCAACAACAACACTATCTC+AGG	-	chr2.1:3235510-3235529	None:intergenic	40.0%
	TCTTATAGAGGGAAACCACT+TGG	+	chr2.1:3235396-3235415	MS.gene61207:CDS	40.0%
!	ACCTGAGATAGTGTTGTTGT+TGG	+	chr2.1:3235506-3235525	MS.gene61207:CDS	40.0%
!	GAAGAGCATGGAGATATCTA+CGG	-	chr2.1:3235313-3235332	None:intergenic	40.0%
!	GATACGGTTAGTCAAGTTAG+GGG	+	chr2.1:3235558-3235577	MS.gene61207:CDS	40.0%
!	TGAGATAGTGTTGTTGTTGG+AGG	+	chr2.1:3235509-3235528	MS.gene61207:CDS	40.0%
	AACCGTACGATCTTGAGGTA+TGG	-	chr2.1:3235249-3235268	None:intergenic	45.0%
	ACGTGGCGGTATCTTATAGA+GGG	+	chr2.1:3235385-3235404	MS.gene61207:CDS	45.0%
	GATGCTGATGCAGAATTCAG+TGG	+	chr2.1:3235471-3235490	MS.gene61207:CDS	45.0%
	GGCTATGGGATCTTCATTTG+TGG	+	chr2.1:3235449-3235468	MS.gene61207:CDS	45.0%
	GTCAAGTTAGGGGCAAAATG+GGG	+	chr2.1:3235568-3235587	MS.gene61207:CDS	45.0%
	TTGTTGGAGGACCTTGCTAA+GGG	+	chr2.1:3235522-3235541	MS.gene61207:CDS	45.0%
!	AGGGTATCATACCCTTAGCA+AGG	-	chr2.1:3235536-3235555	None:intergenic	45.0%
	CACTCTCAACGTGAAGAGCA+TGG	-	chr2.1:3235325-3235344	None:intergenic	50.0%
	CATGCTCTTCACGTTGAGAG+TGG	+	chr2.1:3235323-3235342	MS.gene61207:CDS	50.0%
	GACGTGGCGGTATCTTATAG+AGG	+	chr2.1:3235384-3235403	MS.gene61207:CDS	50.0%
	GCTTCAACCGTACGATCTTG+AGG	-	chr2.1:3235254-3235273	None:intergenic	50.0%
	GGCACGTGACTTCGAAGAAT+GGG	+	chr2.1:3235418-3235437	MS.gene61207:CDS	50.0%
	GTCAACAGCACCAAAGTCCA+TGG	-	chr2.1:3235367-3235386	None:intergenic	50.0%
	GTTGTTGGAGGACCTTGCTA+AGG	+	chr2.1:3235521-3235540	MS.gene61207:CDS	50.0%
	TTCGAAGAATGGGCACGTGA+GGG	+	chr2.1:3235428-3235447	MS.gene61207:CDS	50.0%
	AATGGGCACGTGAGGGCTAT+GGG	+	chr2.1:3235435-3235454	MS.gene61207:CDS	55.0%
	CTTCGAAGAATGGGCACGTG+AGG	+	chr2.1:3235427-3235446	MS.gene61207:CDS	55.0%
	GGGCACGTGACTTCGAAGAA+TGG	+	chr2.1:3235417-3235436	MS.gene61207:CDS	55.0%
	TTCGAAGTCACGTGCCCAAG+TGG	-	chr2.1:3235414-3235433	None:intergenic	55.0%
!!	CTTTGGTGCTGTTGACGTGG+CGG	+	chr2.1:3235371-3235390	MS.gene61207:CDS	55.0%
!!	GGACTTTGGTGCTGTTGACG+TGG	+	chr2.1:3235368-3235387	MS.gene61207:CDS	55.0%
	GAATGGGCACGTGAGGGCTA+TGG	+	chr2.1:3235434-3235453	MS.gene61207:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	3235222	3235733	3235222	ID=MS.gene61207
chr2.1	mRNA	3235222	3235733	3235222	ID=MS.gene61207.t1;Parent=MS.gene61207
chr2.1	exon	3235222	3235617	3235222	ID=MS.gene61207.t1.exon1;Parent=MS.gene61207.t1
chr2.1	CDS	3235222	3235617	3235222	ID=cds.MS.gene61207.t1;Parent=MS.gene61207.t1
chr2.1	exon	3235707	3235733	3235707	ID=MS.gene61207.t1.exon2;Parent=MS.gene61207.t1
chr2.1	CDS	3235707	3235733	3235707	ID=cds.MS.gene61207.t1;Parent=MS.gene61207.t1

Gene Sequence

>MS.gene61207

ATGTATATTTTCTATCCGTCAGATCCATACCTCAAGATCGTACGGTTGAAGCTGAAGAAGATTAAGGTACATCGTGTGCCGCATATCACCGTAGATATCTCCATGCTCTTCACGTTGAGAGTGGAAAATGTTGACGTTTATTCCATGGACTTTGGTGCTGTTGACGTGGCGGTATCTTATAGAGGGAAACCACTTGGGCACGTGACTTCGAAGAATGGGCACGTGAGGGCTATGGGATCTTCATTTGTGGATGCTGATGCAGAATTCAGTGGTATTGGTGTGTTACCTGAGATAGTGTTGTTGTTGGAGGACCTTGCTAAGGGTATGATACCCTTTGATACGGTTAGTCAAGTTAGGGGCAAAATGGGGATTCTATTCTTTTACTTTCCCATTAAGGTAAATCATTTTCTTACTTCCAAGTTATTCATAATCATATGTTGCTTTTTAGTGCTTCTCAATTATTATTTTTTAATAATTTGAGTTAGGTTATAGTTTATGGTGCTAATTTATGA

Protein sequence

>MS.gene61207.t1

MYIFYPSDPYLKIVRLKLKKIKVHRVPHITVDISMLFTLRVENVDVYSMDFGAVDVAVSYRGKPLGHVTSKNGHVRAMGSSFVDADAEFSGIGVLPEIVLLLEDLAKGMIPFDTVSQVRGKMGILFFYFPIKVIVYGANL