AlfalfaGEDB | MS.gene61522

M. sativa cultivar XinJiangDaYe / MS.gene61522

KEGG: -
GO: -
PFAM: PF03168.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61522.t1	AES68023.2	98.1	156	3	0	1	156	1	156	1.50E-79	305.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61522.t1	G7II42	98.1	156	3	0	1	156	1	156	1.1e-79	305.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene61522	0.809071	2.27E-50	-1.69E-46
MS.gene050815	MS.gene61522	0.810739	9.92E-51	-1.69E-46
MS.gene05288	MS.gene61522	0.841227	5.40E-58	-1.69E-46
MS.gene05289	MS.gene61522	0.816771	4.66E-52	-1.69E-46
MS.gene053008	MS.gene61522	0.808066	3.72E-50	-1.69E-46
MS.gene054713	MS.gene61522	0.823194	1.58E-53	-1.69E-46
MS.gene054775	MS.gene61522	0.815483	9.04E-52	-1.69E-46
MS.gene054801	MS.gene61522	0.815339	9.73E-52	-1.69E-46
MS.gene055466	MS.gene61522	0.804231	2.39E-49	-1.69E-46
MS.gene055608	MS.gene61522	0.844095	9.35E-59	-1.69E-46
MS.gene055818	MS.gene61522	0.80324	3.83E-49	-1.69E-46
MS.gene057693	MS.gene61522	0.813126	3.00E-51	-1.69E-46
MS.gene058168	MS.gene61522	0.803798	2.93E-49	-1.69E-46
MS.gene059842	MS.gene61522	0.807973	3.89E-50	-1.69E-46
MS.gene060322	MS.gene61522	0.815116	1.09E-51	-1.69E-46
MS.gene060429	MS.gene61522	0.80687	6.67E-50	-1.69E-46
MS.gene060802	MS.gene61522	0.803822	2.90E-49	-1.69E-46
MS.gene060803	MS.gene61522	0.815442	9.22E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61522.t1	MTR_2g102080	98.077	156	3	0	1	156	1	156	4.00e-111	314
MS.gene61522.t1	MTR_4g012450	39.744	156	91	2	1	154	34	188	2.74e-38	129
MS.gene61522.t1	MTR_6g086455	36.943	157	94	2	1	156	78	230	3.35e-32	115

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61522.t1	AT1G52330	54.902	153	69	1	153	59	211	4.46e-63	193
MS.gene61522.t1	AT1G52330	52.985	134	63	1	134	59	192	6.27e-51	162
MS.gene61522.t1	AT4G13270	43.791	153	86	1	153	60	212	2.50e-44	145

Find 50 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAAATGATTTACCTTAA+TGG	0.212531	2.3:-2070313	None:intergenic
AGAAAATGATTTACCTTAAT+GGG	0.264332	2.3:-2070312	None:intergenic
TAAACTATCATGTGCGGTTT+TGG	0.284881	2.3:+2070457	MS.gene61522:CDS
TAGTCAAGTTAGGGGCAAAA+TGG	0.292543	2.3:+2070269	MS.gene61522:CDS
TTGATACGGTTAGTCAAGTT+AGG	0.332171	2.3:+2070259	MS.gene61522:CDS
GTTGAAGCTGAAGAAGATTA+AGG	0.365406	2.3:+2069969	MS.gene61522:CDS
AGTCAAGTTAGGGGCAAAAT+GGG	0.396713	2.3:+2070270	MS.gene61522:CDS
GAATGGGCACGTGAGGGCTA+TGG	0.456284	2.3:+2070137	MS.gene61522:CDS
TGATACGGTTAGTCAAGTTA+GGG	0.457996	2.3:+2070260	MS.gene61522:CDS
GGCTATGGGATCTTCATTTG+TGG	0.458085	2.3:+2070152	MS.gene61522:CDS
CCTCATGAAGACAGTGTTGA+CGG	0.462375	2.3:-2070505	None:intergenic
TCCAACAACAACACTATCTC+AGG	0.473538	2.3:-2070210	None:intergenic
GGCACGTGACTTCGAAGAAT+GGG	0.476369	2.3:+2070121	MS.gene61522:CDS
ACCTGAGATAGTGTTGTTGT+TGG	0.476726	2.3:+2070209	MS.gene61522:CDS
AATGGGCACGTGAGGGCTAT+GGG	0.477387	2.3:+2070138	MS.gene61522:CDS
AAATGTTGACGTTTATTCCA+TGG	0.481008	2.3:+2070050	MS.gene61522:CDS
GGGCACGTGACTTCGAAGAA+TGG	0.502859	2.3:+2070120	MS.gene61522:CDS
GTTGTTGGAGGACCTTGCTA+AGG	0.507070	2.3:+2070224	MS.gene61522:CDS
GATGCAGAATTCAGTGGTAT+TGG	0.514947	2.3:+2070180	MS.gene61522:CDS
GACGTTTATTCCATGGACTT+TGG	0.517964	2.3:+2070057	MS.gene61522:CDS
AACCGTACGATCTTGAGGTA+TGG	0.521084	2.3:-2069949	None:intergenic
TCTTATAGAGGGAAACCACT+TGG	0.536597	2.3:+2070099	MS.gene61522:CDS
GGGTATGATACCCTTTGATA+CGG	0.543076	2.3:+2070245	MS.gene61522:CDS
TGAGATAGTGTTGTTGTTGG+AGG	0.545985	2.3:+2070212	MS.gene61522:CDS
CTTCGAAGAATGGGCACGTG+AGG	0.549210	2.3:+2070130	MS.gene61522:CDS
TTGTTGGAGGACCTTGCTAA+GGG	0.551961	2.3:+2070225	MS.gene61522:CDS
CACTCTCAACGTGAAGAGCA+TGG	0.555106	2.3:-2070025	None:intergenic
GTCAACAGCACCAAAGTCCA+TGG	0.556982	2.3:-2070067	None:intergenic
AACTTGACTAACCGTATCAA+AGG	0.559559	2.3:-2070256	None:intergenic
ACTTGACTAACCGTATCAAA+GGG	0.561858	2.3:-2070255	None:intergenic
ATCCATACCTCAAGATCGTA+CGG	0.564124	2.3:+2069947	MS.gene61522:CDS
GCTTCAACCGTACGATCTTG+AGG	0.564157	2.3:-2069954	None:intergenic
ATCTTGAGGTATGGATCTGA+CGG	0.564383	2.3:-2069940	None:intergenic
CATGCTCTTCACGTTGAGAG+TGG	0.566163	2.3:+2070026	MS.gene61522:CDS
GACGTGGCGGTATCTTATAG+AGG	0.568477	2.3:+2070087	MS.gene61522:CDS
TTCGAAGTCACGTGCCCAAG+TGG	0.585354	2.3:-2070114	None:intergenic
CTTATAGAGGGAAACCACTT+GGG	0.598189	2.3:+2070100	MS.gene61522:CDS
TTCGAAGAATGGGCACGTGA+GGG	0.610158	2.3:+2070131	MS.gene61522:CDS
GAAGAGCATGGAGATATCTA+CGG	0.612242	2.3:-2070013	None:intergenic
GATACGGTTAGTCAAGTTAG+GGG	0.618576	2.3:+2070261	MS.gene61522:CDS
GGACTTTGGTGCTGTTGACG+TGG	0.632167	2.3:+2070071	MS.gene61522:CDS
AGGGTATCATACCCTTAGCA+AGG	0.651835	2.3:-2070236	None:intergenic
CCGTCAACACTGTCTTCATG+AGG	0.652674	2.3:+2070505	MS.gene61522:CDS
GAGATATCTACGGTGATATG+CGG	0.652943	2.3:-2070003	None:intergenic
ACGTGGCGGTATCTTATAGA+GGG	0.653035	2.3:+2070088	MS.gene61522:CDS
GTCAAGTTAGGGGCAAAATG+GGG	0.655267	2.3:+2070271	MS.gene61522:CDS
ATAGGCTAAACTATCATGTG+CGG	0.659678	2.3:+2070451	MS.gene61522:intron
TGACGGATAGAAAATATACA+TGG	0.674941	2.3:-2069923	None:intergenic
CTTTGGTGCTGTTGACGTGG+CGG	0.678358	2.3:+2070074	MS.gene61522:CDS
GATGCTGATGCAGAATTCAG+TGG	0.701186	2.3:+2070174	MS.gene61522:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTAATAATTTGAGTT+AGG	+	chr2.3:2070388-2070407	MS.gene61522:intron	10.0%
!!	AAGAAAATGATTTACCTTAA+TGG	-	chr2.3:2070316-2070335	None:intergenic	20.0%
!!	ACATATGATTATGAATAACT+TGG	-	chr2.3:2070343-2070362	None:intergenic	20.0%
!!	AGAAAATGATTTACCTTAAT+GGG	-	chr2.3:2070315-2070334	None:intergenic	20.0%
!!!	ATGATAATTTTCCCAATAAT+AGG	+	chr2.3:2070433-2070452	MS.gene61522:intron	20.0%
!	ATGATAGTTTAGCCTATTAT+TGG	-	chr2.3:2070448-2070467	None:intergenic	25.0%
!	TGATAGTTTAGCCTATTATT+GGG	-	chr2.3:2070447-2070466	None:intergenic	25.0%
!	TTGAGTTAGGTTATAGTTTA+TGG	+	chr2.3:2070401-2070420	MS.gene61522:intron	25.0%
!!	ATTCTTTTACTTTCCCATTA+AGG	+	chr2.3:2070299-2070318	MS.gene61522:CDS	25.0%
	AAATGTTGACGTTTATTCCA+TGG	+	chr2.3:2070050-2070069	MS.gene61522:CDS	30.0%
	AACTTGACTAACCGTATCAA+AGG	-	chr2.3:2070259-2070278	None:intergenic	35.0%
	ACTTGACTAACCGTATCAAA+GGG	-	chr2.3:2070258-2070277	None:intergenic	35.0%
	ATAGGCTAAACTATCATGTG+CGG	+	chr2.3:2070451-2070470	MS.gene61522:intron	35.0%
	GTTGAAGCTGAAGAAGATTA+AGG	+	chr2.3:2069969-2069988	MS.gene61522:CDS	35.0%
!	TAAACTATCATGTGCGGTTT+TGG	+	chr2.3:2070457-2070476	MS.gene61522:CDS	35.0%
!	TGATACGGTTAGTCAAGTTA+GGG	+	chr2.3:2070260-2070279	MS.gene61522:CDS	35.0%
!	TTGATACGGTTAGTCAAGTT+AGG	+	chr2.3:2070259-2070278	MS.gene61522:CDS	35.0%
	AGTCAAGTTAGGGGCAAAAT+GGG	+	chr2.3:2070270-2070289	MS.gene61522:CDS	40.0%
	ATCCATACCTCAAGATCGTA+CGG	+	chr2.3:2069947-2069966	MS.gene61522:CDS	40.0%
	ATCTTGAGGTATGGATCTGA+CGG	-	chr2.3:2069943-2069962	None:intergenic	40.0%
	CTTATAGAGGGAAACCACTT+GGG	+	chr2.3:2070100-2070119	MS.gene61522:CDS	40.0%
	GACGTTTATTCCATGGACTT+TGG	+	chr2.3:2070057-2070076	MS.gene61522:CDS	40.0%
	GAGATATCTACGGTGATATG+CGG	-	chr2.3:2070006-2070025	None:intergenic	40.0%
	GATGCAGAATTCAGTGGTAT+TGG	+	chr2.3:2070180-2070199	MS.gene61522:CDS	40.0%
	GGGTATGATACCCTTTGATA+CGG	+	chr2.3:2070245-2070264	MS.gene61522:CDS	40.0%
	TAGTCAAGTTAGGGGCAAAA+TGG	+	chr2.3:2070269-2070288	MS.gene61522:CDS	40.0%
	TCCAACAACAACACTATCTC+AGG	-	chr2.3:2070213-2070232	None:intergenic	40.0%
	TCTTATAGAGGGAAACCACT+TGG	+	chr2.3:2070099-2070118	MS.gene61522:CDS	40.0%
!	ACCTGAGATAGTGTTGTTGT+TGG	+	chr2.3:2070209-2070228	MS.gene61522:CDS	40.0%
!	GAAGAGCATGGAGATATCTA+CGG	-	chr2.3:2070016-2070035	None:intergenic	40.0%
!	GATACGGTTAGTCAAGTTAG+GGG	+	chr2.3:2070261-2070280	MS.gene61522:CDS	40.0%
!	TGAGATAGTGTTGTTGTTGG+AGG	+	chr2.3:2070212-2070231	MS.gene61522:CDS	40.0%
	AACCGTACGATCTTGAGGTA+TGG	-	chr2.3:2069952-2069971	None:intergenic	45.0%
	ACGTGGCGGTATCTTATAGA+GGG	+	chr2.3:2070088-2070107	MS.gene61522:CDS	45.0%
	GATGCTGATGCAGAATTCAG+TGG	+	chr2.3:2070174-2070193	MS.gene61522:CDS	45.0%
	GGCTATGGGATCTTCATTTG+TGG	+	chr2.3:2070152-2070171	MS.gene61522:CDS	45.0%
	GTCAAGTTAGGGGCAAAATG+GGG	+	chr2.3:2070271-2070290	MS.gene61522:CDS	45.0%
	TTGTTGGAGGACCTTGCTAA+GGG	+	chr2.3:2070225-2070244	MS.gene61522:CDS	45.0%
!	AGGGTATCATACCCTTAGCA+AGG	-	chr2.3:2070239-2070258	None:intergenic	45.0%
	CACTCTCAACGTGAAGAGCA+TGG	-	chr2.3:2070028-2070047	None:intergenic	50.0%
	CATGCTCTTCACGTTGAGAG+TGG	+	chr2.3:2070026-2070045	MS.gene61522:CDS	50.0%
	GACGTGGCGGTATCTTATAG+AGG	+	chr2.3:2070087-2070106	MS.gene61522:CDS	50.0%
	GCTTCAACCGTACGATCTTG+AGG	-	chr2.3:2069957-2069976	None:intergenic	50.0%
	GGCACGTGACTTCGAAGAAT+GGG	+	chr2.3:2070121-2070140	MS.gene61522:CDS	50.0%
	GTCAACAGCACCAAAGTCCA+TGG	-	chr2.3:2070070-2070089	None:intergenic	50.0%
	GTTGTTGGAGGACCTTGCTA+AGG	+	chr2.3:2070224-2070243	MS.gene61522:CDS	50.0%
	TTCGAAGAATGGGCACGTGA+GGG	+	chr2.3:2070131-2070150	MS.gene61522:CDS	50.0%
	AATGGGCACGTGAGGGCTAT+GGG	+	chr2.3:2070138-2070157	MS.gene61522:CDS	55.0%
	CTTCGAAGAATGGGCACGTG+AGG	+	chr2.3:2070130-2070149	MS.gene61522:CDS	55.0%
	GGGCACGTGACTTCGAAGAA+TGG	+	chr2.3:2070120-2070139	MS.gene61522:CDS	55.0%
	TTCGAAGTCACGTGCCCAAG+TGG	-	chr2.3:2070117-2070136	None:intergenic	55.0%
!!	CTTTGGTGCTGTTGACGTGG+CGG	+	chr2.3:2070074-2070093	MS.gene61522:CDS	55.0%
!!	GGACTTTGGTGCTGTTGACG+TGG	+	chr2.3:2070071-2070090	MS.gene61522:CDS	55.0%
	GAATGGGCACGTGAGGGCTA+TGG	+	chr2.3:2070137-2070156	MS.gene61522:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2069925	2070526	2069925	ID=MS.gene61522
chr2.3	mRNA	2069925	2070526	2069925	ID=MS.gene61522.t1;Parent=MS.gene61522
chr2.3	exon	2069925	2070320	2069925	ID=MS.gene61522.t1.exon1;Parent=MS.gene61522.t1
chr2.3	CDS	2069925	2070320	2069925	ID=cds.MS.gene61522.t1;Parent=MS.gene61522.t1
chr2.3	exon	2070455	2070526	2070455	ID=MS.gene61522.t1.exon2;Parent=MS.gene61522.t1
chr2.3	CDS	2070455	2070526	2070455	ID=cds.MS.gene61522.t1;Parent=MS.gene61522.t1

Gene Sequence

>MS.gene61522

ATGTATATTTTCTATCCGTCAGATCCATACCTCAAGATCGTACGGTTGAAGCTGAAGAAGATTAAGGTACATCGTGTGCCGCATATCACCGTAGATATCTCCATGCTCTTCACGTTGAGAGTGGAAAATGTTGACGTTTATTCCATGGACTTTGGTGCTGTTGACGTGGCGGTATCTTATAGAGGGAAACCACTTGGGCACGTGACTTCGAAGAATGGGCACGTGAGGGCTATGGGATCTTCATTTGTGGATGCTGATGCAGAATTCAGTGGTATTGGTGTGTTACCTGAGATAGTGTTGTTGTTGGAGGACCTTGCTAAGGGTATGATACCCTTTGATACGGTTAGTCAAGTTAGGGGCAAAATGGGGATTCTATTCTTTTACTTTCCCATTAAGGTAAATCATTTTCTTACTTCCAAGTTATTCATAATCATATGTTGCTTTTTAGTGCTTCTCAATTATTATTTTTTAATAATTTGAGTTAGGTTATAGTTTATGGTGCTAATTTATGATAATTTTCCCAATAATAGGCTAAACTATCATGTGCGGTTTTGGTGAGTACGATAAATCAGACAATTATCCGTCAACACTGTCTTCATGAG

Protein sequence

>MS.gene61522.t1

MYIFYPSDPYLKIVRLKLKKIKVHRVPHITVDISMLFTLRVENVDVYSMDFGAVDVAVSYRGKPLGHVTSKNGHVRAMGSSFVDADAEFSGIGVLPEIVLLLEDLAKGMIPFDTVSQVRGKMGILFFYFPIKAKLSCAVLVSTINQTIIRQHCLHE