Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61727.t1 | XP_003609737.1 | 95.5 | 176 | 8 | 0 | 1 | 176 | 201 | 376 | 1.40E-94 | 355.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61727.t1 | A0A1C9CX66 | 40.4 | 171 | 100 | 2 | 3 | 172 | 221 | 390 | 8.4e-32 | 138.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61727.t1 | G7JMR9 | 95.5 | 176 | 8 | 0 | 1 | 176 | 201 | 376 | 1.0e-94 | 355.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene61727 | 0.817259 | 3.62E-52 | -1.69E-46 |
| MS.gene050020 | MS.gene61727 | 0.844698 | 6.43E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene61727 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene61727 | 0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene61727 | 0.815333 | 9.75E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene61727 | 0.82419 | 9.23E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene61727 | 0.820209 | 7.74E-53 | -1.69E-46 |
| MS.gene052879 | MS.gene61727 | 0.802947 | 4.41E-49 | -1.69E-46 |
| MS.gene055912 | MS.gene61727 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene61727 | 0.807948 | 3.94E-50 | -1.69E-46 |
| MS.gene061176 | MS.gene61727 | 0.96526 | 2.25E-124 | -1.69E-46 |
| MS.gene061177 | MS.gene61727 | 0.965048 | 4.21E-124 | -1.69E-46 |
| MS.gene061178 | MS.gene61727 | 0.965116 | 3.44E-124 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61727.t1 | MTR_4g121590 | 95.455 | 176 | 8 | 0 | 1 | 176 | 201 | 376 | 7.03e-126 | 358 |
| MS.gene61727.t1 | MTR_4g121570 | 77.714 | 175 | 39 | 0 | 1 | 175 | 201 | 375 | 9.81e-103 | 300 |
| MS.gene61727.t1 | MTR_4g073090 | 78.409 | 176 | 38 | 0 | 1 | 176 | 201 | 376 | 3.12e-102 | 299 |
| MS.gene61727.t1 | MTR_3g013890 | 40.805 | 174 | 87 | 3 | 7 | 172 | 225 | 390 | 4.31e-42 | 145 |
| MS.gene61727.t1 | MTR_3g102730 | 40.805 | 174 | 87 | 3 | 7 | 172 | 225 | 390 | 4.70e-42 | 145 |
| MS.gene61727.t1 | MTR_3g102760 | 40.116 | 172 | 101 | 2 | 2 | 172 | 220 | 390 | 9.38e-41 | 141 |
| MS.gene61727.t1 | MTR_1g018610 | 40.351 | 171 | 101 | 1 | 2 | 172 | 254 | 423 | 1.10e-40 | 142 |
| MS.gene61727.t1 | MTR_1g018580 | 39.766 | 171 | 102 | 1 | 2 | 172 | 195 | 364 | 5.54e-40 | 139 |
| MS.gene61727.t1 | MTR_3g102770 | 39.759 | 166 | 99 | 1 | 7 | 172 | 225 | 389 | 1.09e-39 | 139 |
| MS.gene61727.t1 | MTR_1g018590 | 39.181 | 171 | 103 | 1 | 2 | 172 | 225 | 394 | 1.59e-39 | 138 |
| MS.gene61727.t1 | MTR_3g463660 | 35.429 | 175 | 107 | 3 | 3 | 172 | 202 | 375 | 6.65e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61727.t1 | AT4G24220 | 35.673 | 171 | 109 | 1 | 2 | 172 | 219 | 388 | 3.05e-36 | 129 |
| MS.gene61727.t1 | AT4G24220 | 35.673 | 171 | 109 | 1 | 2 | 172 | 218 | 387 | 3.82e-36 | 129 |
| MS.gene61727.t1 | AT5G58750 | 32.571 | 175 | 114 | 3 | 3 | 173 | 204 | 378 | 1.53e-23 | 95.9 |
| MS.gene61727.t1 | AT5G58750 | 32.571 | 175 | 114 | 3 | 3 | 173 | 212 | 386 | 1.64e-23 | 95.9 |
Find 0 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTTAATAGTGTGAAATTCT+GGG | + | 32467:11909-11928 | MS.gene61727:CDS | 25.0% |
| CGTTTAATAGTGTGAAATTC+TGG | + | 32467:11908-11927 | MS.gene61727:CDS | 30.0% | |
| TGAAAAGGAAGAGTTTGATT+TGG | + | 32467:11702-11721 | MS.gene61727:CDS | 30.0% | |
| TGAAACTCAAATCGAAAAAC+TGG | - | 32467:11832-11851 | MS.gene61727:intergenic | 30.0% | |
| ! | ACAAGCAATTTTCTCCAATT+TGG | - | 32467:11794-11813 | MS.gene61727:intergenic | 30.0% |
| CAAGCGTTTAATTGCACAAA+TGG | + | 32467:11607-11626 | MS.gene61727:CDS | 35.0% | |
| CATTTGTGCAATTAAACGCT+TGG | - | 32467:11609-11628 | MS.gene61727:intergenic | 35.0% | |
| CTATTTCCTGGATAACGAAA+CGG | - | 32467:11496-11515 | MS.gene61727:intergenic | 35.0% | |
| TGGACTTCATAAGACCAAAT+TGG | + | 32467:11777-11796 | MS.gene61727:CDS | 35.0% | |
| !! | TTTTCACATGGAAGAGTATG+TGG | + | 32467:11635-11654 | MS.gene61727:CDS | 35.0% |
| !!! | AATTGCTTGTTTTGAAGCCT+TGG | + | 32467:11804-11823 | MS.gene61727:CDS | 35.0% |
| !!! | GACTTTTGCTGTTAAGTTTG+TGG | + | 32467:11672-11691 | MS.gene61727:CDS | 35.0% |
| AAATCGAAAAACTGGCACCA+AGG | - | 32467:11824-11843 | MS.gene61726:intergenic | 40.0% | |
| CGTTTCGTTATCCAGGAAAT+AGG | + | 32467:11494-11513 | MS.gene61727:CDS | 40.0% | |
| GTGCAGTTTATGCGTGATAA+GGG | + | 32467:11724-11743 | MS.gene61727:CDS | 40.0% | |
| GTTTGTGGAGTTGGATGAAA+AGG | + | 32467:11687-11706 | MS.gene61727:CDS | 40.0% | |
| TCCAAAGTGTACCTATTTCC+TGG | - | 32467:11508-11527 | MS.gene61727:intergenic | 40.0% | |
| TCCAGGAAATAGGTACACTT+TGG | + | 32467:11504-11523 | MS.gene61727:CDS | 40.0% | |
| TGCTGTTAAGTTTGTGGAGT+TGG | + | 32467:11678-11697 | MS.gene61727:CDS | 40.0% | |
| ! | GCAATTAAACGCTTGGTTCT+TGG | - | 32467:11602-11621 | MS.gene61727:intergenic | 40.0% |
| ! | TGAGATTTGGGATCAGATTG+TGG | + | 32467:11747-11766 | MS.gene61727:CDS | 40.0% |
| !! | CAAATGGTGACGTTTTCACA+TGG | + | 32467:11623-11642 | MS.gene61727:CDS | 40.0% |
| AACGAAACGGTAATCCTACC+TGG | - | 32467:11483-11502 | MS.gene61726:intergenic | 45.0% | |
| AATTCTGGGTGATGAAGCTG+AGG | + | 32467:11923-11942 | MS.gene61727:CDS | 45.0% | |
| ATTCTGGGTGATGAAGCTGA+GGG | + | 32467:11924-11943 | MS.gene61727:CDS | 45.0% | |
| GATCAGATTGTGGAGGAGTA+TGG | + | 32467:11757-11776 | MS.gene61727:CDS | 45.0% | |
| GCGTGATAAGGGTGAGATTT+GGG | + | 32467:11735-11754 | MS.gene61727:CDS | 45.0% | |
| GGATTACCGTTTCGTTATCC+AGG | + | 32467:11487-11506 | MS.gene61727:CDS | 45.0% | |
| GGTGCAGTTTATGCGTGATA+AGG | + | 32467:11723-11742 | MS.gene61727:CDS | 45.0% | |
| TAATCCTACCTGGCGACATA+TGG | - | 32467:11473-11492 | MS.gene61727:intergenic | 45.0% | |
| TGCGTGATAAGGGTGAGATT+TGG | + | 32467:11734-11753 | MS.gene61727:CDS | 45.0% | |
| ! | CACATGGAAGAGTATGTGGA+AGG | + | 32467:11639-11658 | MS.gene61727:CDS | 45.0% |
| ! | GATTTGGGATCAGATTGTGG+AGG | + | 32467:11750-11769 | MS.gene61727:CDS | 45.0% |
| ! | GGAGCATTTCTGCGATATGA+CGG | + | 32467:11525-11544 | MS.gene61727:CDS | 45.0% |
| ! | GTGGGCTGCAGTTACAAAGA+AGG | + | 32467:11576-11595 | MS.gene61727:CDS | 50.0% |
| GCTCGCAAAGCAGCATGTGT+GGG | + | 32467:11558-11577 | MS.gene61727:CDS | 55.0% | |
| TGCTCGCAAAGCAGCATGTG+TGG | + | 32467:11557-11576 | MS.gene61727:CDS | 55.0% | |
| ATGCGCTGCCATATGTCGCC+AGG | + | 32467:11462-11481 | MS.gene61727:CDS | 60.0% | |
| GCTGCCATATGTCGCCAGGT+AGG | + | 32467:11466-11485 | MS.gene61727:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32467 | gene | 11448 | 11978 | 11448 | ID=MS.gene61727 |
| 32467 | mRNA | 11448 | 11978 | 11448 | ID=MS.gene61727.t1;Parent=MS.gene61727 |
| 32467 | exon | 11448 | 11978 | 11448 | ID=MS.gene61727.t1.exon1;Parent=MS.gene61727.t1 |
| 32467 | CDS | 11448 | 11978 | 11448 | ID=cds.MS.gene61727.t1;Parent=MS.gene61727.t1 |
| Gene Sequence |
| Protein sequence |
