Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61768.t1 | RHN40328.1 | 98.5 | 1102 | 17 | 0 | 1 | 1102 | 1 | 1102 | 0.00E+00 | 2055.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61768.t1 | Q8RX56 | 69.8 | 1107 | 319 | 10 | 1 | 1100 | 1 | 1099 | 0.0e+00 | 1479.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61768.t1 | A0A396GIY6 | 98.5 | 1102 | 17 | 0 | 1 | 1102 | 1 | 1102 | 0.0e+00 | 2055.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene61768 | 0.81342 | 2.58E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene61768 | 0.80122 | 9.98E-49 | -1.69E-46 |
| MS.gene050439 | MS.gene61768 | 0.806125 | 9.57E-50 | -1.69E-46 |
| MS.gene051102 | MS.gene61768 | 0.811235 | 7.75E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene61768 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene61768 | 0.809754 | 1.62E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene61768 | 0.819172 | 1.34E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene61768 | 0.8061 | 9.69E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene61768 | 0.812405 | 4.31E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene61768 | 0.813122 | 3.00E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene61768 | 0.805555 | 1.26E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene61768 | 0.815088 | 1.11E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene61768 | 0.80903 | 2.31E-50 | -1.69E-46 |
| MS.gene054824 | MS.gene61768 | 0.8086 | 2.86E-50 | -1.69E-46 |
| MS.gene055313 | MS.gene61768 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene61768 | 0.81507 | 1.12E-51 | -1.69E-46 |
| MS.gene055469 | MS.gene61768 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene61768 | 0.811411 | 7.10E-51 | -1.69E-46 |
| MS.gene057446 | MS.gene61768 | 0.811782 | 5.89E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene61768 | 0.816141 | 6.44E-52 | -1.69E-46 |
| MS.gene059713 | MS.gene61768 | 0.805587 | 1.24E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene61768 | 0.80689 | 6.60E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene61768 | 0.812471 | 4.17E-51 | -1.69E-46 |
| MS.gene060373 | MS.gene61768 | 0.80941 | 1.92E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61768.t1 | MTR_8g040190 | 98.911 | 1102 | 12 | 0 | 1 | 1102 | 1 | 1102 | 0.0 | 2224 |
| MS.gene61768.t1 | MTR_3g109630 | 31.558 | 995 | 571 | 22 | 181 | 1099 | 36 | 996 | 1.97e-142 | 454 |
| MS.gene61768.t1 | MTR_1g114270 | 31.257 | 947 | 571 | 22 | 189 | 1098 | 16 | 919 | 3.50e-127 | 411 |
| MS.gene61768.t1 | MTR_1g044480 | 29.842 | 888 | 549 | 19 | 261 | 1099 | 170 | 1032 | 9.71e-114 | 378 |
| MS.gene61768.t1 | MTR_1g073760 | 28.159 | 1005 | 601 | 23 | 190 | 1099 | 36 | 1014 | 1.36e-111 | 372 |
| MS.gene61768.t1 | MTR_6g043090 | 28.041 | 888 | 568 | 22 | 256 | 1099 | 285 | 1145 | 1.30e-98 | 338 |
| MS.gene61768.t1 | MTR_1g114190 | 23.754 | 682 | 378 | 20 | 291 | 959 | 810 | 1362 | 4.28e-35 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61768.t1 | AT5G06970 | 70.334 | 1109 | 310 | 11 | 1 | 1100 | 1 | 1099 | 0.0 | 1477 |
| MS.gene61768.t1 | AT4G11670 | 33.008 | 1127 | 675 | 21 | 9 | 1101 | 4 | 1084 | 1.06e-180 | 559 |
| MS.gene61768.t1 | AT4G11670 | 32.626 | 1131 | 669 | 21 | 9 | 1101 | 4 | 1079 | 4.05e-176 | 547 |
| MS.gene61768.t1 | AT4G11670 | 32.010 | 1159 | 676 | 22 | 9 | 1101 | 4 | 1116 | 3.78e-174 | 543 |
| MS.gene61768.t1 | AT4G11670 | 32.024 | 1171 | 672 | 23 | 9 | 1101 | 4 | 1128 | 8.24e-174 | 542 |
| MS.gene61768.t1 | AT2G25800 | 30.847 | 992 | 563 | 27 | 188 | 1099 | 37 | 985 | 1.04e-132 | 428 |
| MS.gene61768.t1 | AT2G20010 | 30.967 | 972 | 580 | 19 | 190 | 1099 | 7 | 949 | 7.65e-130 | 419 |
| MS.gene61768.t1 | AT2G20010 | 31.512 | 860 | 533 | 15 | 267 | 1099 | 1 | 831 | 1.16e-122 | 397 |
| MS.gene61768.t1 | AT2G33420 | 30.395 | 885 | 544 | 20 | 261 | 1099 | 179 | 1037 | 1.58e-119 | 394 |
| MS.gene61768.t1 | AT1G04470 | 29.505 | 888 | 546 | 19 | 261 | 1099 | 177 | 1033 | 6.53e-113 | 376 |
Find 252 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTAGTTCACAAACTTTA+TGG | 0.122588 | 8.4:+58504931 | MS.gene61768:CDS |
| CTTATAGGGTTCAGCAATTA+TGG | 0.183898 | 8.4:+58504019 | MS.gene61768:intron |
| TTATATAAACCCAGTGTTTC+AGG | 0.191950 | 8.4:+58509568 | MS.gene61768:CDS |
| CATAAAGTCTGACATTAAAT+TGG | 0.220443 | 8.4:-58501412 | None:intergenic |
| ACGAGATATGTCTTTCTTAC+AGG | 0.226698 | 8.4:+58503195 | MS.gene61768:CDS |
| CTGTAGGAATTCTTTATATC+TGG | 0.262965 | 8.4:+58511251 | MS.gene61768:intron |
| GATCAGTCATTTGAATAAAT+TGG | 0.275299 | 8.4:+58508528 | MS.gene61768:CDS |
| GTCGATCCAGCTAAAAGTTT+TGG | 0.280708 | 8.4:+58500748 | MS.gene61768:intron |
| TTATATAAGTTGTCGATAAA+AGG | 0.287570 | 8.4:-58509553 | None:intergenic |
| GAAAAGGATGGATACTCTTT+TGG | 0.287580 | 8.4:+58500517 | MS.gene61768:CDS |
| ATCTGATCACGATCGCTAAT+TGG | 0.293446 | 8.4:-58504231 | None:intergenic |
| CAGAATGCTTGAGGTCTCTA+AGG | 0.304013 | 8.4:+58501312 | MS.gene61768:CDS |
| TGCCGCTGTTGAAGAGATAT+TGG | 0.308586 | 8.4:-58506896 | None:intergenic |
| GTCACTGATCCGGAGTCTTC+AGG | 0.315415 | 8.4:+58498381 | MS.gene61768:CDS |
| TTGCTCCGTGTTATCTTAGA+TGG | 0.323281 | 8.4:+58510433 | MS.gene61768:CDS |
| ATTGGAGTATGATACCTTTA+GGG | 0.323934 | 8.4:-58510515 | None:intergenic |
| AGAGATTATCATGATCATAC+TGG | 0.327787 | 8.4:+58497651 | MS.gene61768:CDS |
| AAACGAGTTGAGGATTCTTT+TGG | 0.333210 | 8.4:+58500826 | MS.gene61768:CDS |
| CAATAACAAACATACCATTT+GGG | 0.345764 | 8.4:-58497679 | None:intergenic |
| TTGAAGGTCTCAGGGCTAAA+AGG | 0.348521 | 8.4:+58498604 | MS.gene61768:CDS |
| ATAGGAGGCACTGCAGAAAT+AGG | 0.352514 | 8.4:-58498435 | None:intergenic |
| AAGCTTGGGCGTAGCAAAAC+TGG | 0.352725 | 8.4:+58499454 | MS.gene61768:CDS |
| TTCAATTCTGTAAACCTCAT+TGG | 0.354808 | 8.4:-58507539 | None:intergenic |
| AAATCAGGTTGCACGCGTTC+GGG | 0.355645 | 8.4:+58511304 | MS.gene61768:CDS |
| TCACATATAAAGTATTTAAC+TGG | 0.356974 | 8.4:-58508051 | None:intergenic |
| AATCACCCTGTGGTTGGATT+TGG | 0.364400 | 8.4:+58500785 | MS.gene61768:CDS |
| ATAAATTGGAAGATAGCATT+TGG | 0.371797 | 8.4:+58508542 | MS.gene61768:CDS |
| AAAATCAGGTTGCACGCGTT+CGG | 0.373952 | 8.4:+58511303 | MS.gene61768:CDS |
| GATAGATGATAAGTCCAAAA+AGG | 0.375003 | 8.4:+58509355 | MS.gene61768:CDS |
| TGCTCTAGACTCTCGGCTGC+TGG | 0.378535 | 8.4:-58505105 | None:intergenic |
| CAAACTTTATGGTGTCAAAC+TGG | 0.381275 | 8.4:+58504942 | MS.gene61768:CDS |
| CTTCCATGGTTGAAGGTCTC+AGG | 0.384597 | 8.4:+58498595 | MS.gene61768:CDS |
| ACTTATAGAACCACTTGATA+TGG | 0.387619 | 8.4:+58509606 | MS.gene61768:CDS |
| ATATTGTTGTGGAGCCTCTC+AGG | 0.388994 | 8.4:+58510297 | MS.gene61768:CDS |
| TTGGCTTGTGCTGGAGCCAC+AGG | 0.389675 | 8.4:+58499281 | MS.gene61768:CDS |
| AATCTTGTTGTTAATGACTT+AGG | 0.390941 | 8.4:+58507605 | MS.gene61768:CDS |
| CTGGAGGTGATGGGCTTCCT+CGG | 0.391647 | 8.4:+58511270 | MS.gene61768:CDS |
| ATTAAATAAGATACCCTTGA+TGG | 0.394167 | 8.4:+58503111 | MS.gene61768:CDS |
| TGGAGGTGATGGGCTTCCTC+GGG | 0.394488 | 8.4:+58511271 | MS.gene61768:CDS |
| TCGGGGAGTTGTCGAAAATC+AGG | 0.395214 | 8.4:+58511289 | MS.gene61768:CDS |
| CTATAGATTGAGACAAAATC+TGG | 0.400047 | 8.4:+58505757 | MS.gene61768:intron |
| CAGTCTTCTTCAAGCCTCAT+TGG | 0.400366 | 8.4:+58510334 | MS.gene61768:CDS |
| TTCTTTATATCTGGAGGTGA+TGG | 0.402557 | 8.4:+58511260 | MS.gene61768:CDS |
| ATCTCGTTCACCTTCAACCT+TGG | 0.402762 | 8.4:-58503179 | None:intergenic |
| CCAGCTCGGGGTGACTTAAC+TGG | 0.404193 | 8.4:+58501362 | MS.gene61768:CDS |
| GGGAGCGATGGACACATAAA+AGG | 0.412176 | 8.4:+58508563 | MS.gene61768:CDS |
| GTGGTTGGATTTGGTGAATC+AGG | 0.413923 | 8.4:+58500794 | MS.gene61768:CDS |
| CACCCTAGAGCCTGAAACAC+TGG | 0.413930 | 8.4:-58509578 | None:intergenic |
| TTCCAACAAGCCAACTAATC+CGG | 0.414480 | 8.4:-58499506 | None:intergenic |
| CGCTGGGTCAATTCCCAGCT+TGG | 0.417220 | 8.4:+58505793 | MS.gene61768:CDS |
| GGATGTCTCTGTTGTAAAAC+AGG | 0.428231 | 8.4:-58504886 | None:intergenic |
| CGCAAAGTGTTGGAGTTGTT+AGG | 0.430231 | 8.4:-58508027 | None:intergenic |
| ATACCAAAGTCTTCTGCATA+AGG | 0.433056 | 8.4:+58512074 | MS.gene61768:CDS |
| CAAAATGCTCCCGGATTAGT+TGG | 0.433806 | 8.4:+58499496 | MS.gene61768:CDS |
| AGCAGCTTGATTAGGAAGCT+TGG | 0.436508 | 8.4:+58499439 | MS.gene61768:CDS |
| AACCTAAAATTCTTCCAAGC+TGG | 0.437031 | 8.4:-58505807 | None:intergenic |
| TTGTCAGCCCATCTCTGAAT+TGG | 0.438195 | 8.4:-58503229 | None:intergenic |
| TTGGCCTCGTTGTTCCATCA+AGG | 0.442036 | 8.4:-58503125 | None:intergenic |
| GAGTCTAGAGCAGTTTATAA+TGG | 0.442443 | 8.4:+58505116 | MS.gene61768:CDS |
| GATTACCAGGAGGCTTCTAC+TGG | 0.442873 | 8.4:+58504064 | MS.gene61768:CDS |
| TCCCGGATTAGTTGGCTTGT+TGG | 0.448769 | 8.4:+58499504 | MS.gene61768:CDS |
| TTCTGTCCTTTGAAGTTCAC+CGG | 0.450638 | 8.4:-58501294 | None:intergenic |
| TTACTGTACCGCGTGAGAAC+AGG | 0.451881 | 8.4:-58507911 | None:intergenic |
| TAAATTGGAAGATAGCATTT+GGG | 0.452772 | 8.4:+58508543 | MS.gene61768:CDS |
| AGGCTTCTGAGTCACTTCTA+TGG | 0.453529 | 8.4:-58512025 | None:intergenic |
| GAGATCCTCTTGGCTTGTGC+TGG | 0.456679 | 8.4:+58499272 | MS.gene61768:CDS |
| GATGGAACAACGAGGCCAAC+AGG | 0.458647 | 8.4:+58503129 | MS.gene61768:CDS |
| CTAGAAATGCAAGGTGGAAA+AGG | 0.458845 | 8.4:+58511960 | MS.gene61768:CDS |
| TGAAGTTCACCGGATGAAGA+TGG | 0.460794 | 8.4:-58501284 | None:intergenic |
| CAGTTTCTCCCATCTTCATC+CGG | 0.463177 | 8.4:+58501275 | MS.gene61768:intron |
| TTTCCTTATGCAGAAGACTT+TGG | 0.466526 | 8.4:-58512077 | None:intergenic |
| AATGCTCTTGTGGGAAAGGC+CGG | 0.466631 | 8.4:+58500494 | MS.gene61768:CDS |
| CTTCAGGTTCACCTCCCAAA+AGG | 0.470142 | 8.4:+58498397 | MS.gene61768:CDS |
| CTTAACACCAATTCAGAGAT+GGG | 0.471689 | 8.4:+58503222 | MS.gene61768:CDS |
| CCGGGAAAGAAAACTCTTGA+AGG | 0.473163 | 8.4:-58510460 | None:intergenic |
| CAAGGTGGAAAAGGCAAACT+AGG | 0.473872 | 8.4:+58511969 | MS.gene61768:CDS |
| ACCTAAAATTCTTCCAAGCT+GGG | 0.479827 | 8.4:-58505806 | None:intergenic |
| GAGGCTTCTACTGGAAGAAC+CGG | 0.481409 | 8.4:+58504073 | MS.gene61768:CDS |
| AAAAGTTATAAATGCTGCTA+TGG | 0.481416 | 8.4:+58509397 | MS.gene61768:CDS |
| GCCGCTGTTGAAGAGATATT+GGG | 0.482781 | 8.4:-58506895 | None:intergenic |
| GCAGCTTGATTAGGAAGCTT+GGG | 0.482955 | 8.4:+58499440 | MS.gene61768:CDS |
| ACCGGAGACGTAGGATAAGC+AGG | 0.482987 | 8.4:-58498477 | None:intergenic |
| AAAATTGTTAGAAGAAGATC+TGG | 0.484529 | 8.4:+58510489 | MS.gene61768:CDS |
| ACTCGCGGAGAGACCAGCTC+GGG | 0.485181 | 8.4:+58501349 | MS.gene61768:CDS |
| CTTGATATATTAGAAGCAAC+CGG | 0.487383 | 8.4:-58498495 | None:intergenic |
| TTAAGTGCAACCTCTCCTGT+TGG | 0.488390 | 8.4:-58503144 | None:intergenic |
| TAGCAGTTGCGATGATTACC+AGG | 0.489673 | 8.4:+58504051 | MS.gene61768:CDS |
| AAATAAAACCCATGCATAGC+AGG | 0.489815 | 8.4:-58502154 | None:intergenic |
| TTGTTAGGACGCTAATTTCA+CGG | 0.491196 | 8.4:-58508012 | None:intergenic |
| GGCACTGCAGAAATAGGAAC+AGG | 0.491930 | 8.4:-58498429 | None:intergenic |
| TATACGATGGCAAAAGAGGC+AGG | 0.494620 | 8.4:+58500598 | MS.gene61768:CDS |
| AACTGGTGAAATATGCCATT+GGG | 0.495679 | 8.4:+58501379 | MS.gene61768:CDS |
| TGAGACAAAATCTGGAACCT+TGG | 0.497734 | 8.4:+58505765 | MS.gene61768:CDS |
| TATCTACTCTTTCAACAACT+TGG | 0.499175 | 8.4:-58504786 | None:intergenic |
| GGTGGAGTGGAAACAGCAAT+AGG | 0.500666 | 8.4:-58498453 | None:intergenic |
| GACGATACTTCCATGGTTGA+AGG | 0.500765 | 8.4:+58498588 | MS.gene61768:CDS |
| TCATCTAGCGTAACTGCGCC+AGG | 0.502197 | 8.4:-58497462 | None:intergenic |
| AAGCTTCCTAATCAAGCTGC+TGG | 0.505731 | 8.4:-58499437 | None:intergenic |
| CCACAACAATATCGCAAAGT+TGG | 0.506570 | 8.4:-58510286 | None:intergenic |
| ATGCTCTTGTGGGAAAGGCC+GGG | 0.507491 | 8.4:+58500495 | MS.gene61768:CDS |
| CTGGTTAATCACCCTGTGGT+TGG | 0.508562 | 8.4:+58500779 | MS.gene61768:CDS |
| TCTTAACACCAATTCAGAGA+TGG | 0.508944 | 8.4:+58503221 | MS.gene61768:CDS |
| TCCTGCTTATCCTACGTCTC+CGG | 0.509250 | 8.4:+58498476 | MS.gene61768:CDS |
| TGTTAGGACGCTAATTTCAC+GGG | 0.510191 | 8.4:-58508011 | None:intergenic |
| TCATACTGGGTTACCCCAAA+TGG | 0.510656 | 8.4:+58497665 | MS.gene61768:CDS |
| TCCATCACACCTGCTATGCA+TGG | 0.510734 | 8.4:+58502145 | MS.gene61768:CDS |
| GAACAATCACCTGTGGAAAA+CGG | 0.511748 | 8.4:-58498666 | None:intergenic |
| AGAAGATCTGGAAGCCCTAA+AGG | 0.511754 | 8.4:+58510501 | MS.gene61768:CDS |
| TTGTGGGAAAGGCCGGGAAA+AGG | 0.512435 | 8.4:+58500501 | MS.gene61768:CDS |
| ACAGAAGTACACACAGTGTC+CGG | 0.514360 | 8.4:-58504092 | None:intergenic |
| TCCAACAAGCCAACTAATCC+GGG | 0.515563 | 8.4:-58499505 | None:intergenic |
| GAAGTTCACCGGATGAAGAT+GGG | 0.518947 | 8.4:-58501283 | None:intergenic |
| CTTCATCCGGTGAACTTCAA+AGG | 0.521626 | 8.4:+58501288 | MS.gene61768:CDS |
| GGGTTGCTAAATGCTCTTGT+GGG | 0.521738 | 8.4:+58500485 | MS.gene61768:CDS |
| ACCCAGTGTTTCAGGCTCTA+GGG | 0.522133 | 8.4:+58509576 | MS.gene61768:CDS |
| CGCAGTTACGCTAGATGATG+TGG | 0.522770 | 8.4:+58497467 | MS.gene61768:CDS |
| CAATTTCCTCAACAAGATCT+CGG | 0.524136 | 8.4:-58505172 | None:intergenic |
| CTGGAACCTTGGTGTTACGC+TGG | 0.524702 | 8.4:+58505776 | MS.gene61768:CDS |
| AGAAGTTTACAGAATTGTAG+AGG | 0.525601 | 8.4:+58506934 | MS.gene61768:CDS |
| TCTTTATATCTGGAGGTGAT+GGG | 0.525643 | 8.4:+58511261 | MS.gene61768:CDS |
| TCTCTTCAACAGCGGCATGC+TGG | 0.525744 | 8.4:+58506902 | MS.gene61768:CDS |
| GCTAAATGCTCTTGTGGGAA+AGG | 0.527309 | 8.4:+58500490 | MS.gene61768:CDS |
| TGCTTGAGCGTGAGGAGACA+AGG | 0.527592 | 8.4:+58507987 | MS.gene61768:CDS |
| AAGATAGCATTTGGGAGCGA+TGG | 0.529677 | 8.4:+58508551 | MS.gene61768:CDS |
| TTCAGGTTCACCTCCCAAAA+GGG | 0.529740 | 8.4:+58498398 | MS.gene61768:CDS |
| GACGTAGGATAAGCAGGAGG+TGG | 0.530557 | 8.4:-58498471 | None:intergenic |
| CAGAGTGACGCAGGTTCAGT+TGG | 0.531247 | 8.4:+58498345 | MS.gene61768:intron |
| CCCATGCATAGCAGGTGTGA+TGG | 0.531431 | 8.4:-58502146 | None:intergenic |
| AGCCCTGAGACCTTCAACCA+TGG | 0.531646 | 8.4:-58498598 | None:intergenic |
| AGCTTGCGTTCCAAGGTTGA+AGG | 0.531998 | 8.4:+58503169 | MS.gene61768:CDS |
| TTCTTCCAGTAGAAGCCTCC+TGG | 0.532875 | 8.4:-58504069 | None:intergenic |
| AAGAGATACTTACTCTTCCA+TGG | 0.536527 | 8.4:-58497253 | None:intergenic |
| GCTCTAGACTCTCGGCTGCT+GGG | 0.537737 | 8.4:-58505104 | None:intergenic |
| CAATGAGGCTTGAAGAAGAC+TGG | 0.538415 | 8.4:-58510333 | None:intergenic |
| CTGCAGAAATAGGAACAGGC+GGG | 0.539263 | 8.4:-58498425 | None:intergenic |
| AAGTATGCGTGTTCAGTTGG+AGG | 0.540724 | 8.4:+58499528 | MS.gene61768:CDS |
| AACCCAGTGTTTCAGGCTCT+AGG | 0.541034 | 8.4:+58509575 | MS.gene61768:CDS |
| TGGCCTCGTTGTTCCATCAA+GGG | 0.541245 | 8.4:-58503124 | None:intergenic |
| GGTGGAGCGAGTTACTCAAC+AGG | 0.542267 | 8.4:+58505831 | MS.gene61768:CDS |
| GGATCTCGTATGCAGTTTCA+CGG | 0.543702 | 8.4:-58499256 | None:intergenic |
| GATGCAGATATCTGAGGCAA+TGG | 0.544309 | 8.4:+58500445 | MS.gene61768:intron |
| AGATGGGCTGACAAGCAGCT+TGG | 0.545212 | 8.4:+58503238 | MS.gene61768:CDS |
| TGCTTCTAATATATCAAGAT+CGG | 0.546451 | 8.4:+58498500 | MS.gene61768:CDS |
| TGTTCTCACGCGGTACAGTA+AGG | 0.547334 | 8.4:+58507913 | MS.gene61768:CDS |
| TTGTGGAGCCTCTCAGGGAT+CGG | 0.547773 | 8.4:+58510303 | MS.gene61768:CDS |
| GACAATGCTCTTCAAGTGTA+CGG | 0.547883 | 8.4:+58507581 | MS.gene61768:CDS |
| CGAGCTGGTCTCTCCGCGAG+TGG | 0.548522 | 8.4:-58501347 | None:intergenic |
| TGGCTTGTGCTGGAGCCACA+GGG | 0.548621 | 8.4:+58499282 | MS.gene61768:CDS |
| TTCTTCTGCAAGCAATGCCA+AGG | 0.549442 | 8.4:-58504824 | None:intergenic |
| AACTGCATACGAGATCCTCT+TGG | 0.549946 | 8.4:+58499262 | MS.gene61768:CDS |
| TAACTGGTGAAATATGCCAT+TGG | 0.552117 | 8.4:+58501378 | MS.gene61768:CDS |
| AGGGTTGCTAAATGCTCTTG+TGG | 0.552444 | 8.4:+58500484 | MS.gene61768:CDS |
| TCACAGTCTCAAAATGCTCC+CGG | 0.552709 | 8.4:+58499487 | MS.gene61768:CDS |
| ATCACGATCGCTAATTGGTA+AGG | 0.552836 | 8.4:-58504226 | None:intergenic |
| CCAACTTTGCGATATTGTTG+TGG | 0.553081 | 8.4:+58510286 | MS.gene61768:CDS |
| ATGTTATCACAAGGGAGCAT+AGG | 0.557302 | 8.4:+58503065 | MS.gene61768:CDS |
| CACTCGCGGAGAGACCAGCT+CGG | 0.557310 | 8.4:+58501348 | MS.gene61768:CDS |
| TTCCATGGTTGAAGGTCTCA+GGG | 0.563715 | 8.4:+58498596 | MS.gene61768:CDS |
| TATAAACTGCTCTAGACTCT+CGG | 0.564594 | 8.4:-58505112 | None:intergenic |
| CGCGTGAGAACAGGCACAGG+TGG | 0.565068 | 8.4:-58507902 | None:intergenic |
| GGCAAAGATTGAGGAATCTG+AGG | 0.566651 | 8.4:+58500847 | MS.gene61768:CDS |
| TGGAACCTTGGTGTTACGCT+GGG | 0.566764 | 8.4:+58505777 | MS.gene61768:CDS |
| CCAGTTAAGTCACCCCGAGC+TGG | 0.567931 | 8.4:-58501362 | None:intergenic |
| ACGCGGTACAGTAAGGAAGC+TGG | 0.570085 | 8.4:+58507920 | MS.gene61768:CDS |
| GAAATATGCCATTGGGCAGA+TGG | 0.572366 | 8.4:+58501386 | MS.gene61768:CDS |
| TTTAACTGGACGCAAAGTGT+TGG | 0.572496 | 8.4:-58508037 | None:intergenic |
| GGAAAGTATGCGTGTTCAGT+TGG | 0.572903 | 8.4:+58499525 | MS.gene61768:CDS |
| TGTTCAGCACTATCCAGAAA+CGG | 0.573586 | 8.4:-58505054 | None:intergenic |
| GATTCACCAAATCCAACCAC+AGG | 0.574311 | 8.4:-58500791 | None:intergenic |
| TTGTTTCTTCAGAAATTGAG+AGG | 0.574868 | 8.4:-58512045 | None:intergenic |
| AGTTTACAGAATTGTAGAGG+AGG | 0.575246 | 8.4:+58506937 | MS.gene61768:CDS |
| GACGCAAGACAAACGAGTTG+AGG | 0.576589 | 8.4:+58500816 | MS.gene61768:CDS |
| TAGGAATTCTTTATATCTGG+AGG | 0.577667 | 8.4:+58511254 | MS.gene61768:intron |
| CACTCGTGTGCTTGAGCGTG+AGG | 0.578334 | 8.4:+58507979 | MS.gene61768:CDS |
| CGGGTTGTGATCAAGTTGCA+TGG | 0.578548 | 8.4:+58511323 | MS.gene61768:CDS |
| GGGAAAGGCCGGGAAAAGGA+TGG | 0.579169 | 8.4:+58500505 | MS.gene61768:CDS |
| TGGCTTTAACAGAGTGACGC+AGG | 0.582418 | 8.4:+58498336 | MS.gene61768:intron |
| TATGTCACATGAGCATCCCT+TGG | 0.582581 | 8.4:+58504807 | MS.gene61768:CDS |
| AGATGACGACGATACTTCCA+TGG | 0.583579 | 8.4:+58498581 | MS.gene61768:CDS |
| CAACCACAGGGTGATTAACC+AGG | 0.587243 | 8.4:-58500778 | None:intergenic |
| GAGGAAATTGAATCTCTACC+AGG | 0.590294 | 8.4:+58505185 | MS.gene61768:CDS |
| ATCTTCTTCAAAGATACAGA+AGG | 0.591854 | 8.4:+58497376 | MS.gene61768:CDS |
| CTCCGTGTTATCTTAGATGG+TGG | 0.593094 | 8.4:+58510436 | MS.gene61768:CDS |
| GATCAAGTTGCATGGCTATG+AGG | 0.593691 | 8.4:+58511331 | MS.gene61768:CDS |
| TATTGTTGTGGAGCCTCTCA+GGG | 0.597161 | 8.4:+58510298 | MS.gene61768:CDS |
| GAGATTATCATGATCATACT+GGG | 0.597495 | 8.4:+58497652 | MS.gene61768:CDS |
| CCATCACACCTGCTATGCAT+GGG | 0.599808 | 8.4:+58502146 | MS.gene61768:CDS |
| ACCCTAGAGCCTGAAACACT+GGG | 0.600099 | 8.4:-58509577 | None:intergenic |
| TTCAAAGATACAGAAGGGAT+AGG | 0.600469 | 8.4:+58497382 | MS.gene61768:CDS |
| TTCAAAGGACAGAATGCTTG+AGG | 0.601000 | 8.4:+58501303 | MS.gene61768:CDS |
| TGCAGAAATAGGAACAGGCG+GGG | 0.603842 | 8.4:-58498424 | None:intergenic |
| AATAACAAACATACCATTTG+GGG | 0.607762 | 8.4:-58497678 | None:intergenic |
| AGGATAAGCAGGAGGTGGAG+TGG | 0.609506 | 8.4:-58498466 | None:intergenic |
| GAAGACTTGAAATCTGCAAG+TGG | 0.611796 | 8.4:+58511936 | MS.gene61768:CDS |
| GGAGATTGCCGTTCCACTCG+CGG | 0.612128 | 8.4:+58501334 | MS.gene61768:CDS |
| ATGGACACATAAAAGGTCAC+AGG | 0.613761 | 8.4:+58508570 | MS.gene61768:CDS |
| TCTTCTTCAAAGATACAGAA+GGG | 0.613959 | 8.4:+58497377 | MS.gene61768:CDS |
| TCGGTCTTAAAGTTCCAATG+AGG | 0.615037 | 8.4:+58507525 | MS.gene61768:CDS |
| TGGTCTCTCCGCGAGTGGAA+CGG | 0.615348 | 8.4:-58501342 | None:intergenic |
| AAGAAAGTTGTAATGCCACC+TGG | 0.616050 | 8.4:+58497444 | MS.gene61768:CDS |
| ATACCTCACTAAAATGCAGA+TGG | 0.616453 | 8.4:-58503267 | None:intergenic |
| ATTGGTAACCATCTGCCCAA+TGG | 0.619789 | 8.4:-58501394 | None:intergenic |
| ATGGATATTAGAACAAAACA+AGG | 0.624343 | 8.4:+58500464 | MS.gene61768:CDS |
| ACCCAATATCTCTTCAACAG+CGG | 0.624424 | 8.4:+58506894 | MS.gene61768:CDS |
| GTTTACAGTATGTTATCACA+AGG | 0.625864 | 8.4:+58503056 | MS.gene61768:intron |
| GGAGGTGAACCTGAAGACTC+CGG | 0.626502 | 8.4:-58498390 | None:intergenic |
| CAGTGTTTCAGGCTCTAGGG+TGG | 0.627323 | 8.4:+58509579 | MS.gene61768:CDS |
| AGAATGCTTGAGGTCTCTAA+GGG | 0.627425 | 8.4:+58501313 | MS.gene61768:CDS |
| AACAACGAGGCCAACAGGAG+AGG | 0.635178 | 8.4:+58503134 | MS.gene61768:CDS |
| GGAGTGGAAACAGCAATAGG+AGG | 0.639496 | 8.4:-58498450 | None:intergenic |
| GGAGGTGATGGGCTTCCTCG+GGG | 0.639585 | 8.4:+58511272 | MS.gene61768:CDS |
| AAAATGCCGAGATCTTGTTG+AGG | 0.642233 | 8.4:+58505166 | MS.gene61768:CDS |
| TATTAAAGTAATAACCAATG+AGG | 0.646151 | 8.4:-58510348 | None:intergenic |
| GTCACTATCCGATCCCTGAG+AGG | 0.647026 | 8.4:-58510311 | None:intergenic |
| GAAGAGCATTGTCAATGCCA+CGG | 0.648397 | 8.4:-58507571 | None:intergenic |
| TACCGCGTGAGAACAGGCAC+AGG | 0.649998 | 8.4:-58507905 | None:intergenic |
| CACCTGTGCCTGTTCTCACG+CGG | 0.655073 | 8.4:+58507903 | MS.gene61768:CDS |
| AAGATCGGAGTCTTTAGACT+CGG | 0.655303 | 8.4:+58498515 | MS.gene61768:CDS |
| GACCACCATCTAAGATAACA+CGG | 0.657667 | 8.4:-58510438 | None:intergenic |
| GAGACATCCTCAAGCAACTG+TGG | 0.657830 | 8.4:+58504900 | MS.gene61768:CDS |
| GCAAGTGGCCTAGAAATGCA+AGG | 0.658732 | 8.4:+58511951 | MS.gene61768:CDS |
| ACGCAGCAAAGCAACTCCAA+AGG | 0.659543 | 8.4:-58500542 | None:intergenic |
| GCAGAAATAGGAACAGGCGG+GGG | 0.659901 | 8.4:-58498423 | None:intergenic |
| ACTGCAGAAATAGGAACAGG+CGG | 0.660859 | 8.4:-58498426 | None:intergenic |
| GCAGATACCACAGTTGCTTG+AGG | 0.666050 | 8.4:-58504907 | None:intergenic |
| TGGCTCCAGCACAAGCCAAG+AGG | 0.672051 | 8.4:-58499277 | None:intergenic |
| TCTTCTGCAAGCAATGCCAA+GGG | 0.677560 | 8.4:-58504823 | None:intergenic |
| TTAGAAGCAACCGGAGACGT+AGG | 0.681205 | 8.4:-58498486 | None:intergenic |
| CTCGCGGAGAGACCAGCTCG+GGG | 0.681496 | 8.4:+58501350 | MS.gene61768:CDS |
| TGTATTTGACATGCTAGACG+AGG | 0.686765 | 8.4:+58501451 | MS.gene61768:CDS |
| GAGCGCATTTGTGAATACAC+CGG | 0.687073 | 8.4:+58509419 | MS.gene61768:CDS |
| GCTAATTGGTAAGGACTGCG+TGG | 0.690362 | 8.4:-58504217 | None:intergenic |
| TTTACAGTATGTTATCACAA+GGG | 0.692220 | 8.4:+58503057 | MS.gene61768:intron |
| GGGCCTGGTTAATCACCCTG+TGG | 0.692301 | 8.4:+58500775 | MS.gene61768:CDS |
| TGTATGGATGCAGATATCTG+AGG | 0.701598 | 8.4:+58500439 | MS.gene61768:intron |
| GAATTGAATAGCATGTTCCG+TGG | 0.704393 | 8.4:+58507554 | MS.gene61768:CDS |
| AGACGAGGGAAAGCTGACAG+AGG | 0.713100 | 8.4:+58501466 | MS.gene61768:CDS |
| GTATTTGACATGCTAGACGA+GGG | 0.714794 | 8.4:+58501452 | MS.gene61768:CDS |
| GGAGCGAGTTACTCAACAGG+AGG | 0.714971 | 8.4:+58505834 | MS.gene61768:CDS |
| TGGATATTAGAACAAAACAA+GGG | 0.716721 | 8.4:+58500465 | MS.gene61768:CDS |
| CAATCACCTGTGGAAAACGG+AGG | 0.722853 | 8.4:-58498663 | None:intergenic |
| TAGTGCTGAACATTTAAGTG+AGG | 0.724046 | 8.4:+58505065 | MS.gene61768:CDS |
| GTTAGGACGCTAATTTCACG+GGG | 0.727786 | 8.4:-58508010 | None:intergenic |
| ATTGACCCAGCGTAACACCA+AGG | 0.727979 | 8.4:-58505782 | None:intergenic |
| CAGTTGCGATGATTACCAGG+AGG | 0.729936 | 8.4:+58504054 | MS.gene61768:CDS |
| TCTAGCGTAACTGCGCCAGG+TGG | 0.733072 | 8.4:-58497459 | None:intergenic |
| TGATTACACAGCAAGCAAGG+AGG | 0.737507 | 8.4:+58507871 | MS.gene61768:intron |
| ATTCACCAAATCCAACCACA+GGG | 0.740962 | 8.4:-58500790 | None:intergenic |
| AGTGGCCTAGAAATGCAAGG+TGG | 0.753482 | 8.4:+58511954 | MS.gene61768:CDS |
| GGAGACGTAGGATAAGCAGG+AGG | 0.780399 | 8.4:-58498474 | None:intergenic |
| ACAAGAAAGAGAGCTAACTG+TGG | 0.782116 | 8.4:+58498539 | MS.gene61768:CDS |
| ATACCCTTGATGGAACAACG+AGG | 0.849454 | 8.4:+58503121 | MS.gene61768:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 58497255 | 58512094 | 58497255 | ID=MS.gene61768 |
| chr8.4 | mRNA | 58497255 | 58512094 | 58497255 | ID=MS.gene61768.t1;Parent=MS.gene61768 |
| chr8.4 | exon | 58497255 | 58497262 | 58497255 | ID=MS.gene61768.t1.exon1;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58497255 | 58497262 | 58497255 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58497362 | 58497537 | 58497362 | ID=MS.gene61768.t1.exon2;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58497362 | 58497537 | 58497362 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58497622 | 58497686 | 58497622 | ID=MS.gene61768.t1.exon3;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58497622 | 58497686 | 58497622 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58498348 | 58498678 | 58498348 | ID=MS.gene61768.t1.exon4;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58498348 | 58498678 | 58498348 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58499237 | 58499303 | 58499237 | ID=MS.gene61768.t1.exon5;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58499237 | 58499303 | 58499237 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58499393 | 58499549 | 58499393 | ID=MS.gene61768.t1.exon6;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58499393 | 58499549 | 58499393 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58500452 | 58500619 | 58500452 | ID=MS.gene61768.t1.exon7;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58500452 | 58500619 | 58500452 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58500758 | 58500868 | 58500758 | ID=MS.gene61768.t1.exon8;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58500758 | 58500868 | 58500758 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58501278 | 58501487 | 58501278 | ID=MS.gene61768.t1.exon9;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58501278 | 58501487 | 58501278 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58502081 | 58502182 | 58502081 | ID=MS.gene61768.t1.exon10;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58502081 | 58502182 | 58502081 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58503064 | 58503285 | 58503064 | ID=MS.gene61768.t1.exon11;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58503064 | 58503285 | 58503064 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58504026 | 58504100 | 58504026 | ID=MS.gene61768.t1.exon12;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58504026 | 58504100 | 58504026 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58504216 | 58504296 | 58504216 | ID=MS.gene61768.t1.exon13;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58504216 | 58504296 | 58504216 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58504781 | 58504963 | 58504781 | ID=MS.gene61768.t1.exon14;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58504781 | 58504963 | 58504781 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58505051 | 58505206 | 58505051 | ID=MS.gene61768.t1.exon15;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58505051 | 58505206 | 58505051 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58505763 | 58505855 | 58505763 | ID=MS.gene61768.t1.exon16;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58505763 | 58505855 | 58505763 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58506887 | 58506958 | 58506887 | ID=MS.gene61768.t1.exon17;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58506887 | 58506958 | 58506887 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58507509 | 58507626 | 58507509 | ID=MS.gene61768.t1.exon18;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58507509 | 58507626 | 58507509 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58507882 | 58508069 | 58507882 | ID=MS.gene61768.t1.exon19;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58507882 | 58508069 | 58507882 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58508523 | 58508604 | 58508523 | ID=MS.gene61768.t1.exon20;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58508523 | 58508604 | 58508523 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58509348 | 58509440 | 58509348 | ID=MS.gene61768.t1.exon21;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58509348 | 58509440 | 58509348 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58509521 | 58509627 | 58509521 | ID=MS.gene61768.t1.exon22;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58509521 | 58509627 | 58509521 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58510278 | 58510355 | 58510278 | ID=MS.gene61768.t1.exon23;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58510278 | 58510355 | 58510278 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58510427 | 58510522 | 58510427 | ID=MS.gene61768.t1.exon24;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58510427 | 58510522 | 58510427 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58511257 | 58511352 | 58511257 | ID=MS.gene61768.t1.exon25;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58511257 | 58511352 | 58511257 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| chr8.4 | exon | 58511921 | 58512094 | 58511921 | ID=MS.gene61768.t1.exon26;Parent=MS.gene61768.t1 |
| chr8.4 | CDS | 58511921 | 58512094 | 58511921 | ID=cds.MS.gene61768.t1;Parent=MS.gene61768.t1 |
| Gene Sequence |
| Protein sequence |
