Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63384.t1 | XP_003596287.1 | 71.5 | 144 | 30 | 1 | 1 | 144 | 1 | 133 | 3.40E-30 | 141.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63384.t1 | G7IR17 | 71.5 | 144 | 30 | 1 | 1 | 144 | 1 | 133 | 2.4e-30 | 141.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene63384 | 0.811323 | 7.41E-51 | -1.69E-46 |
| MS.gene050883 | MS.gene63384 | 0.828516 | 8.61E-55 | -1.69E-46 |
| MS.gene05141 | MS.gene63384 | 0.80081 | 1.21E-48 | -1.69E-46 |
| MS.gene051946 | MS.gene63384 | 0.819357 | 1.21E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene63384 | 0.825086 | 5.68E-54 | -1.69E-46 |
| MS.gene054708 | MS.gene63384 | 0.804419 | 2.18E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene63384 | 0.845264 | 4.53E-59 | -1.69E-46 |
| MS.gene056311 | MS.gene63384 | 0.843486 | 1.36E-58 | -1.69E-46 |
| MS.gene056313 | MS.gene63384 | 0.826339 | 2.86E-54 | -1.69E-46 |
| MS.gene057014 | MS.gene63384 | 0.830966 | 2.18E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene63384 | -0.808485 | 3.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63384.t1 | MTR_2g075540 | 66.892 | 148 | 30 | 2 | 1 | 144 | 1 | 133 | 4.25e-48 | 151 |
| MS.gene63384.t1 | MTR_2g075590 | 62.838 | 148 | 34 | 3 | 1 | 144 | 1 | 131 | 1.21e-43 | 140 |
| MS.gene63384.t1 | MTR_2g075600 | 51.829 | 164 | 57 | 2 | 1 | 144 | 1 | 162 | 2.16e-42 | 138 |
| MS.gene63384.t1 | MTR_2g075550 | 51.408 | 142 | 49 | 4 | 1 | 124 | 1 | 140 | 1.16e-29 | 105 |
| MS.gene63384.t1 | MTR_2g075570 | 56.557 | 122 | 40 | 3 | 1 | 113 | 1 | 118 | 3.13e-23 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCACAGGAAGCGGAGGATT+GGG | 0.335111 | 2.4:-21803635 | MS.gene63384:CDS |
| GGTGGATTCTTTCGATTACT+TGG | 0.383958 | 2.4:+21803545 | None:intergenic |
| TAGCACAGGAAGCGGAGGAT+TGG | 0.389817 | 2.4:-21803636 | MS.gene63384:CDS |
| AGCACAGGCGGAGGAGGATT+GGG | 0.392310 | 2.4:-21803518 | MS.gene63384:CDS |
| TAGCACAGGCGGAGGAGGAT+TGG | 0.413090 | 2.4:-21803519 | MS.gene63384:CDS |
| CTCCGCTTCCTGTGCTACCC+GGG | 0.415668 | 2.4:+21803642 | None:intergenic |
| CCTCCTCCGCCTGTGCTACC+TGG | 0.415974 | 2.4:+21803524 | None:intergenic |
| CCTCCGCTTCCTGTGCTACC+CGG | 0.427212 | 2.4:+21803641 | None:intergenic |
| GGCGGAGGAGGATTGGGAAC+TGG | 0.445749 | 2.4:-21803512 | MS.gene63384:CDS |
| GGGTTGGCAGAGCTGGTATG+TGG | 0.457229 | 2.4:+21804120 | None:intergenic |
| TTTCAAATGGCTCTTGCTGC+AGG | 0.461051 | 2.4:-21804152 | None:intergenic |
| CCAGGTAGCACAGGCGGAGG+AGG | 0.487226 | 2.4:-21803524 | MS.gene63384:CDS |
| GGTTGGCAGAGCTGGTATGT+GGG | 0.497814 | 2.4:+21804121 | None:intergenic |
| TCCGCTTCCTGTGCTACCCG+GGG | 0.514938 | 2.4:+21803643 | None:intergenic |
| ATTGGGGACTGGTCCGAAAA+CGG | 0.529672 | 2.4:-21803618 | MS.gene63384:CDS |
| GCACAGGAAGCGGAGGATTG+GGG | 0.533668 | 2.4:-21803634 | MS.gene63384:CDS |
| TGCACAAACAACTAGTACTC+AGG | 0.551675 | 2.4:-21803489 | MS.gene63384:CDS |
| GGAAGCGGAGGATTGGGGAC+TGG | 0.557972 | 2.4:-21803629 | MS.gene63384:CDS |
| AAGAATCCACCAGGTAGCAC+AGG | 0.570025 | 2.4:-21803533 | MS.gene63384:CDS |
| TCAATCTTACTGCCAATGAG+TGG | 0.586404 | 2.4:-21803427 | MS.gene63384:CDS |
| AACAACTAGTACTCAGGTGG+AGG | 0.594211 | 2.4:-21803483 | MS.gene63384:CDS |
| CCTCCGCCTGTGCTACCTGG+TGG | 0.595864 | 2.4:+21803527 | None:intergenic |
| CCACCAGGTAGCACAGGCGG+AGG | 0.597247 | 2.4:-21803527 | MS.gene63384:CDS |
| CCGGGTAGCACAGGAAGCGG+AGG | 0.602411 | 2.4:-21803641 | MS.gene63384:CDS |
| AATTGATCGAAAGAAACCCC+CGG | 0.603280 | 2.4:-21803660 | MS.gene63384:CDS |
| CAATCTTACTGCCAATGAGT+GGG | 0.616463 | 2.4:-21803426 | MS.gene63384:CDS |
| GAGAATACTCAGCGACGTGG+AGG | 0.622518 | 2.4:-21803596 | MS.gene63384:CDS |
| AATCCACCAGGTAGCACAGG+CGG | 0.634430 | 2.4:-21803530 | MS.gene63384:CDS |
| CAATGTCGAACCTACAAAGA+GGG | 0.635757 | 2.4:+21803382 | None:intergenic |
| ACAATGTCGAACCTACAAAG+AGG | 0.639877 | 2.4:+21803381 | None:intergenic |
| AGTAATCGAAAGAATCCACC+AGG | 0.647364 | 2.4:-21803542 | MS.gene63384:CDS |
| CCGCTTCCTGTGCTACCCGG+GGG | 0.651421 | 2.4:+21803644 | None:intergenic |
| TCTTACTGCCAATGAGTGGG+TGG | 0.702286 | 2.4:-21803423 | MS.gene63384:CDS |
| ACGGAGAATACTCAGCGACG+TGG | 0.705185 | 2.4:-21803599 | MS.gene63384:CDS |
| ACAAACAACTAGTACTCAGG+TGG | 0.706249 | 2.4:-21803486 | MS.gene63384:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTATAATAAACTATCAT+TGG | - | chr2.4:21803748-21803767 | MS.gene63384:intron | 15.0% |
| !!! | AATAAATTTTTAACTTGTTC+AGG | + | chr2.4:21803652-21803671 | None:intergenic | 15.0% |
| !!! | AGATTTTATAAATCAGTATA+GGG | - | chr2.4:21803707-21803726 | MS.gene63384:intron | 15.0% |
| !!! | ATAAATTTTTAACTTGTTCA+GGG | + | chr2.4:21803651-21803670 | None:intergenic | 15.0% |
| !!! | TACTGATTTATAAAATCTAT+AGG | + | chr2.4:21803705-21803724 | None:intergenic | 15.0% |
| !!! | TAGATTTTATAAATCAGTAT+AGG | - | chr2.4:21803706-21803725 | MS.gene63384:intron | 15.0% |
| !! | TAATTATATACGTATGCTTA+AGG | - | chr2.4:21803486-21803505 | MS.gene63384:CDS | 20.0% |
| !!! | GTAATTGTCATAGATTTTTT+AGG | + | chr2.4:21803430-21803449 | None:intergenic | 20.0% |
| ! | TTTAAATTAGCCATGATTCA+TGG | - | chr2.4:21803602-21803621 | MS.gene63384:CDS | 25.0% |
| !!! | AGTATAGGGAATTTTTTGTA+TGG | - | chr2.4:21803721-21803740 | MS.gene63384:intron | 25.0% |
| !!! | TGTTTTGTTTTTCTTGTTGT+TGG | + | chr2.4:21803831-21803850 | None:intergenic | 25.0% |
| AAAACCACCAAAAAATTCTG+AGG | - | chr2.4:21803943-21803962 | MS.gene63384:intron | 30.0% | |
| GTTAATCAAACCATGAATCA+TGG | + | chr2.4:21803615-21803634 | None:intergenic | 30.0% | |
| !!! | AGATTTTTTAGGCACATAGT+TGG | + | chr2.4:21803419-21803438 | None:intergenic | 30.0% |
| !!! | ATTTTTTAGGCACATAGTTG+GGG | + | chr2.4:21803417-21803436 | None:intergenic | 30.0% |
| !!! | GATTTTTTAGGCACATAGTT+GGG | + | chr2.4:21803418-21803437 | None:intergenic | 30.0% |
| !!! | CTTTTTTTGACCCTCTTTGT+AGG | - | chr2.4:21804121-21804140 | MS.gene63384:CDS | 35.0% |
| !!! | TTTTTTAGGCACATAGTTGG+GGG | + | chr2.4:21803416-21803435 | None:intergenic | 35.0% |
| AAAAATCACCACCCACTCAT+TGG | + | chr2.4:21804101-21804120 | None:intergenic | 40.0% | |
| AATTGATCGAAAGAAACCCC+CGG | - | chr2.4:21803853-21803872 | MS.gene63384:intron | 40.0% | |
| ACAAACAACTAGTACTCAGG+TGG | - | chr2.4:21804027-21804046 | MS.gene63384:intron | 40.0% | |
| ACAATGTCGAACCTACAAAG+AGG | + | chr2.4:21804135-21804154 | None:intergenic | 40.0% | |
| AGTAATCGAAAGAATCCACC+AGG | - | chr2.4:21803971-21803990 | MS.gene63384:intron | 40.0% | |
| CAATCTTACTGCCAATGAGT+GGG | - | chr2.4:21804087-21804106 | MS.gene63384:CDS | 40.0% | |
| CAATGTCGAACCTACAAAGA+GGG | + | chr2.4:21804134-21804153 | None:intergenic | 40.0% | |
| TCAATCTTACTGCCAATGAG+TGG | - | chr2.4:21804086-21804105 | MS.gene63384:CDS | 40.0% | |
| TGCACAAACAACTAGTACTC+AGG | - | chr2.4:21804024-21804043 | MS.gene63384:intron | 40.0% | |
| ! | GGTGGATTCTTTCGATTACT+TGG | + | chr2.4:21803971-21803990 | None:intergenic | 40.0% |
| !!! | CTTGGTGCCTCAGAATTTTT+TGG | + | chr2.4:21803953-21803972 | None:intergenic | 40.0% |
| AACAACTAGTACTCAGGTGG+AGG | - | chr2.4:21804030-21804049 | MS.gene63384:intron | 45.0% | |
| ATTGATCGAAAGAAACCCCC+GGG | - | chr2.4:21803854-21803873 | MS.gene63384:intron | 45.0% | |
| ! | CGCTGAGTATTCTCCGTTTT+CGG | + | chr2.4:21803911-21803930 | None:intergenic | 45.0% |
| !!! | GGTGCCTCAGAATTTTTTGG+TGG | + | chr2.4:21803950-21803969 | None:intergenic | 45.0% |
| AAGAATCCACCAGGTAGCAC+AGG | - | chr2.4:21803980-21803999 | MS.gene63384:intron | 50.0% | |
| TCTTACTGCCAATGAGTGGG+TGG | - | chr2.4:21804090-21804109 | MS.gene63384:CDS | 50.0% | |
| TTAGGCACATAGTTGGGGGT+TGG | + | chr2.4:21803412-21803431 | None:intergenic | 50.0% | |
| !! | ATTGGGGACTGGTCCGAAAA+CGG | - | chr2.4:21803895-21803914 | MS.gene63384:intron | 50.0% |
| AATCCACCAGGTAGCACAGG+CGG | - | chr2.4:21803983-21804002 | MS.gene63384:intron | 55.0% | |
| ACGGAGAATACTCAGCGACG+TGG | - | chr2.4:21803914-21803933 | MS.gene63384:intron | 55.0% | |
| AGCACAGGAAGCGGAGGATT+GGG | - | chr2.4:21803878-21803897 | MS.gene63384:intron | 55.0% | |
| GAGAATACTCAGCGACGTGG+AGG | - | chr2.4:21803917-21803936 | MS.gene63384:intron | 55.0% | |
| GGTTGGCAGAGCTGGTATGT+GGG | + | chr2.4:21803395-21803414 | None:intergenic | 55.0% | |
| TAGCACAGGAAGCGGAGGAT+TGG | - | chr2.4:21803877-21803896 | MS.gene63384:intron | 55.0% | |
| AAGAAACCCCCGGGTAGCAC+AGG | - | chr2.4:21803863-21803882 | MS.gene63384:intron | 60.0% | |
| AGCACAGGCGGAGGAGGATT+GGG | - | chr2.4:21803995-21804014 | MS.gene63384:intron | 60.0% | |
| GCACAGGAAGCGGAGGATTG+GGG | - | chr2.4:21803879-21803898 | MS.gene63384:intron | 60.0% | |
| TAGCACAGGCGGAGGAGGAT+TGG | - | chr2.4:21803994-21804013 | MS.gene63384:intron | 60.0% | |
| ! | GGGTTGGCAGAGCTGGTATG+TGG | + | chr2.4:21803396-21803415 | None:intergenic | 60.0% |
| ! | TAGTTGGGGGTTGGCAGAGC+TGG | + | chr2.4:21803403-21803422 | None:intergenic | 60.0% |
| CCTCCGCTTCCTGTGCTACC+CGG | + | chr2.4:21803875-21803894 | None:intergenic | 65.0% | |
| CTCCGCTTCCTGTGCTACCC+GGG | + | chr2.4:21803874-21803893 | None:intergenic | 65.0% | |
| GGAAGCGGAGGATTGGGGAC+TGG | - | chr2.4:21803884-21803903 | MS.gene63384:intron | 65.0% | |
| GGCGGAGGAGGATTGGGAAC+TGG | - | chr2.4:21804001-21804020 | MS.gene63384:intron | 65.0% | |
| TCCGCTTCCTGTGCTACCCG+GGG | + | chr2.4:21803873-21803892 | None:intergenic | 65.0% | |
| CCACCAGGTAGCACAGGCGG+AGG | - | chr2.4:21803986-21804005 | MS.gene63384:intron | 70.0% | |
| CCAGGTAGCACAGGCGGAGG+AGG | - | chr2.4:21803989-21804008 | MS.gene63384:intron | 70.0% | |
| CCCCCGGGTAGCACAGGAAG+CGG | - | chr2.4:21803869-21803888 | MS.gene63384:intron | 70.0% | |
| CCGCTTCCTGTGCTACCCGG+GGG | + | chr2.4:21803872-21803891 | None:intergenic | 70.0% | |
| CCGGGTAGCACAGGAAGCGG+AGG | - | chr2.4:21803872-21803891 | MS.gene63384:intron | 70.0% | |
| CCTCCGCCTGTGCTACCTGG+TGG | + | chr2.4:21803989-21804008 | None:intergenic | 70.0% | |
| CCTCCTCCGCCTGTGCTACC+TGG | + | chr2.4:21803992-21804011 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 21803367 | 21804168 | 21803367 | ID=MS.gene63384 |
| chr2.4 | mRNA | 21803367 | 21804168 | 21803367 | ID=MS.gene63384.t1;Parent=MS.gene63384 |
| chr2.4 | exon | 21804083 | 21804168 | 21804083 | ID=MS.gene63384.t1.exon1;Parent=MS.gene63384.t1 |
| chr2.4 | CDS | 21804083 | 21804168 | 21804083 | ID=cds.MS.gene63384.t1;Parent=MS.gene63384.t1 |
| chr2.4 | exon | 21803367 | 21803715 | 21803367 | ID=MS.gene63384.t1.exon2;Parent=MS.gene63384.t1 |
| chr2.4 | CDS | 21803367 | 21803715 | 21803367 | ID=cds.MS.gene63384.t1;Parent=MS.gene63384.t1 |
| Gene Sequence |
| Protein sequence |
