Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63818.t1 | XP_024640815.1 | 62.6 | 334 | 67 | 8 | 1 | 290 | 1 | 320 | 2.90E-49 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63818.t1 | G7K4I5 | 58.2 | 359 | 69 | 9 | 1 | 290 | 1 | 347 | 3.5e-44 | 188.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene63818 | 0.823147 | 1.62E-53 | -1.69E-46 |
| MS.gene050807 | MS.gene63818 | 0.804747 | 1.86E-49 | -1.69E-46 |
| MS.gene050959 | MS.gene63818 | 0.806247 | 9.02E-50 | -1.69E-46 |
| MS.gene051626 | MS.gene63818 | 0.82541 | 4.76E-54 | -1.69E-46 |
| MS.gene051789 | MS.gene63818 | 0.819227 | 1.30E-52 | -1.69E-46 |
| MS.gene052207 | MS.gene63818 | 0.814021 | 1.90E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene63818 | 0.812834 | 3.47E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene63818 | 0.817436 | 3.30E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene63818 | 0.812561 | 3.99E-51 | -1.69E-46 |
| MS.gene054184 | MS.gene63818 | 0.810021 | 1.42E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene63818 | 0.815616 | 8.44E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene63818 | 0.81698 | 4.18E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene63818 | -0.833554 | 4.99E-56 | -1.69E-46 |
| MS.gene057260 | MS.gene63818 | 0.804162 | 2.47E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene63818 | 0.8209 | 5.37E-53 | -1.69E-46 |
| MS.gene058749 | MS.gene63818 | 0.803335 | 3.66E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene63818 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene059124 | MS.gene63818 | 0.811207 | 7.86E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene63818 | 0.840411 | 8.84E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63818.t1 | MTR_5g094950 | 57.103 | 359 | 73 | 8 | 1 | 290 | 1 | 347 | 1.22e-105 | 322 |
| MS.gene63818.t1 | MTR_5g094970 | 51.104 | 317 | 69 | 8 | 70 | 316 | 6 | 306 | 1.97e-75 | 237 |
| MS.gene63818.t1 | MTR_5g095050 | 36.945 | 563 | 160 | 17 | 1 | 372 | 1 | 559 | 1.34e-64 | 215 |
| MS.gene63818.t1 | MTR_5g094960 | 40.854 | 328 | 107 | 9 | 1 | 290 | 1 | 279 | 4.87e-52 | 180 |
| MS.gene63818.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 1.33e-49 | 174 |
| MS.gene63818.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 2.26e-49 | 174 |
| MS.gene63818.t1 | MTR_5g094920 | 34.596 | 396 | 107 | 9 | 1 | 290 | 1 | 350 | 1.55e-38 | 145 |
| MS.gene63818.t1 | MTR_5g094990 | 40.705 | 312 | 124 | 13 | 1 | 290 | 1 | 273 | 1.52e-36 | 138 |
| MS.gene63818.t1 | MTR_5g095370 | 41.206 | 199 | 70 | 5 | 1 | 196 | 1 | 155 | 8.24e-32 | 120 |
| MS.gene63818.t1 | MTR_5g094910 | 34.731 | 334 | 104 | 11 | 1 | 301 | 1 | 253 | 3.04e-28 | 114 |
| MS.gene63818.t1 | MTR_5g095400 | 46.341 | 164 | 56 | 3 | 26 | 185 | 1 | 136 | 1.10e-27 | 114 |
| MS.gene63818.t1 | MTR_5g095430 | 45.806 | 155 | 52 | 3 | 35 | 185 | 1 | 127 | 9.89e-27 | 111 |
| MS.gene63818.t1 | MTR_5g077500 | 45.122 | 164 | 59 | 4 | 178 | 311 | 97 | 259 | 1.67e-24 | 102 |
| MS.gene63818.t1 | MTR_5g095390 | 44.086 | 186 | 67 | 5 | 30 | 202 | 20 | 181 | 2.64e-21 | 94.4 |
| MS.gene63818.t1 | MTR_5g095310 | 39.456 | 147 | 61 | 3 | 35 | 179 | 1 | 121 | 1.66e-19 | 89.4 |
| MS.gene63818.t1 | MTR_5g095360 | 41.121 | 107 | 52 | 3 | 61 | 158 | 1 | 105 | 1.61e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGACCCATGTTCGATTT+TGG | 0.133002 | 5.3:-75889884 | None:intergenic |
| CTCAAAACCATTACATGTTA+AGG | 0.367230 | 5.3:+75890899 | MS.gene63818:CDS |
| TGAGTTAGATAATGAAGTTT+TGG | 0.377487 | 5.3:+75890443 | MS.gene63818:CDS |
| GTCATTTCTTCTAAGTTATG+AGG | 0.381921 | 5.3:-75889955 | None:intergenic |
| CTTACAACACAACGTTGAAA+TGG | 0.390778 | 5.3:+75890089 | MS.gene63818:CDS |
| TTGACATCCTTAACATGTAA+TGG | 0.392334 | 5.3:-75890906 | None:intergenic |
| GGGCTGCTTGCATCTTTGCC+TGG | 0.393057 | 5.3:-75890990 | None:intergenic |
| GAGAGGAACTTTGTCAGAAA+GGG | 0.410933 | 5.3:+75890154 | MS.gene63818:CDS |
| TTAGCTGATAGAGTCACTAT+TGG | 0.415973 | 5.3:+75890312 | MS.gene63818:CDS |
| TGAGCTAGAAGCTAAAGTTT+TGG | 0.417553 | 5.3:+75890530 | MS.gene63818:CDS |
| CCAATCTTCGGGTCACTCTC+TGG | 0.419534 | 5.3:-75891060 | None:intergenic |
| AAGAAAGAAGTACGTTGAGT+TGG | 0.438659 | 5.3:+75890341 | MS.gene63818:CDS |
| GGAGGATGTTGAAGCAAGTC+AGG | 0.455376 | 5.3:+75890734 | MS.gene63818:CDS |
| CGAGCTTCGAAGTAAATTGA+AGG | 0.461338 | 5.3:+75890602 | MS.gene63818:CDS |
| GACACATGAGGTGTTGCAAC+CGG | 0.466770 | 5.3:-75890777 | None:intergenic |
| AGAGAGGAACTTTGTCAGAA+AGG | 0.484616 | 5.3:+75890153 | MS.gene63818:CDS |
| ATGTCAAGAAAGCCAAAGTC+AGG | 0.490710 | 5.3:+75890922 | MS.gene63818:CDS |
| GAAGATGGTGATGAGGTTGA+TGG | 0.497731 | 5.3:+75891023 | MS.gene63818:CDS |
| TCTAACACTAGACCTGACTT+TGG | 0.506997 | 5.3:-75890934 | None:intergenic |
| AGCAAGAATCACGTAGTTGG+TGG | 0.527719 | 5.3:-75889908 | None:intergenic |
| AACAAGATGATTGATGGTCC+AGG | 0.535173 | 5.3:+75890972 | MS.gene63818:CDS |
| GAGGTTGATGGTATTGATGA+TGG | 0.540674 | 5.3:+75891035 | MS.gene63818:CDS |
| CTTATTCGAGTCTTTGAACA+CGG | 0.544179 | 5.3:+75890677 | MS.gene63818:CDS |
| TTCAGCAAGAATCACGTAGT+TGG | 0.558811 | 5.3:-75889911 | None:intergenic |
| GCATGAAAGAAATGCAAGGT+TGG | 0.577609 | 5.3:+75890713 | MS.gene63818:CDS |
| TGAGTTGAGGAAACTGTTTG+AGG | 0.587379 | 5.3:+75890272 | MS.gene63818:CDS |
| TGAAGTTGTAACAGATATGA+TGG | 0.587982 | 5.3:+75889930 | MS.gene63818:CDS |
| TCACCATCTTCAATCACAAG+TGG | 0.589505 | 5.3:-75891011 | None:intergenic |
| AGCCCACTTGTGATTGAAGA+TGG | 0.597478 | 5.3:+75891008 | MS.gene63818:CDS |
| GAAAGAGTTTCAGAAAAGAG+AGG | 0.598100 | 5.3:+75890137 | MS.gene63818:CDS |
| TACTATAAATGAGAAGCGTA+AGG | 0.599833 | 5.3:+75890647 | MS.gene63818:CDS |
| ATTGAACCCAAAATCGAACA+TGG | 0.604289 | 5.3:+75889877 | MS.gene63818:CDS |
| GTACGTTGAGTTGGAGTGTG+AGG | 0.619740 | 5.3:+75890350 | MS.gene63818:CDS |
| TGATCATGTTGCAAATGCAC+CGG | 0.620646 | 5.3:+75890758 | MS.gene63818:CDS |
| GCAACACCTCATGTGTCTGA+TGG | 0.630963 | 5.3:+75890783 | MS.gene63818:CDS |
| GAGAGAGGAACATTGAGTTG+AGG | 0.631931 | 5.3:+75890259 | MS.gene63818:CDS |
| TGAAAGAAATGCAAGGTTGG+AGG | 0.632876 | 5.3:+75890716 | MS.gene63818:CDS |
| TCTTTGAACACGGAGTTCAG+AGG | 0.634013 | 5.3:+75890687 | MS.gene63818:CDS |
| TTGAAATGGAGAAACTTGCA+AGG | 0.634595 | 5.3:+75890103 | MS.gene63818:CDS |
| CACCATCTTCAATCACAAGT+GGG | 0.636322 | 5.3:-75891010 | None:intergenic |
| CTGTTGTAGTACTAAGAGAG+AGG | 0.656292 | 5.3:+75890244 | MS.gene63818:CDS |
| TGTGATTGAAGATGGTGATG+AGG | 0.677350 | 5.3:+75891016 | MS.gene63818:CDS |
| GATTGCATGAAAGAAATGCA+AGG | 0.680697 | 5.3:+75890709 | MS.gene63818:CDS |
| TTGAACCCAAAATCGAACAT+GGG | 0.683973 | 5.3:+75889878 | MS.gene63818:CDS |
| TTTGTACCATCAGACACATG+AGG | 0.707522 | 5.3:-75890789 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTTAAGTTATATTTTAC+AGG | + | chr5.3:75890863-75890882 | MS.gene63818:intron | 15.0% |
| ! | TTGAAAAACAAGATGATTGA+TGG | + | chr5.3:75890966-75890985 | MS.gene63818:CDS | 25.0% |
| !! | GAAGTACTAAGAAAAAAGAA+TGG | + | chr5.3:75890420-75890439 | MS.gene63818:CDS | 25.0% |
| !! | TGAGTTAGATAATGAAGTTT+TGG | + | chr5.3:75890443-75890462 | MS.gene63818:CDS | 25.0% |
| CTCAAAACCATTACATGTTA+AGG | + | chr5.3:75890899-75890918 | MS.gene63818:CDS | 30.0% | |
| GAAGTTGAAAGAAAAATGTG+TGG | + | chr5.3:75890377-75890396 | MS.gene63818:CDS | 30.0% | |
| GTCATTTCTTCTAAGTTATG+AGG | - | chr5.3:75889958-75889977 | None:intergenic | 30.0% | |
| TGAAGTTGTAACAGATATGA+TGG | + | chr5.3:75889930-75889949 | MS.gene63818:CDS | 30.0% | |
| TTGACATCCTTAACATGTAA+TGG | - | chr5.3:75890909-75890928 | None:intergenic | 30.0% | |
| ! | TACTATAAATGAGAAGCGTA+AGG | + | chr5.3:75890647-75890666 | MS.gene63818:CDS | 30.0% |
| ! | TGGAGATTTTGAATGAGAAA+TGG | + | chr5.3:75890556-75890575 | MS.gene63818:CDS | 30.0% |
| ! | TTTGGAGTTGAAGCAAAAAA+GGG | + | chr5.3:75890461-75890480 | MS.gene63818:CDS | 30.0% |
| !!! | TTTTGGAGTTGAAGCAAAAA+AGG | + | chr5.3:75890460-75890479 | MS.gene63818:CDS | 30.0% |
| AAAAATGTGTGGAGGATGAT+AGG | + | chr5.3:75890388-75890407 | MS.gene63818:CDS | 35.0% | |
| ATTGAACCCAAAATCGAACA+TGG | + | chr5.3:75889877-75889896 | MS.gene63818:CDS | 35.0% | |
| CTTACAACACAACGTTGAAA+TGG | + | chr5.3:75890089-75890108 | MS.gene63818:CDS | 35.0% | |
| CTTATTCGAGTCTTTGAACA+CGG | + | chr5.3:75890677-75890696 | MS.gene63818:CDS | 35.0% | |
| GAAAGAGTTTCAGAAAAGAG+AGG | + | chr5.3:75890137-75890156 | MS.gene63818:CDS | 35.0% | |
| GATTGCATGAAAGAAATGCA+AGG | + | chr5.3:75890709-75890728 | MS.gene63818:CDS | 35.0% | |
| GTTGAAAGAAAAATGTGTGG+AGG | + | chr5.3:75890380-75890399 | MS.gene63818:CDS | 35.0% | |
| TTAGCTGATAGAGTCACTAT+TGG | + | chr5.3:75890312-75890331 | MS.gene63818:CDS | 35.0% | |
| TTGAAATGGAGAAACTTGCA+AGG | + | chr5.3:75890103-75890122 | MS.gene63818:CDS | 35.0% | |
| TTGAACCCAAAATCGAACAT+GGG | + | chr5.3:75889878-75889897 | MS.gene63818:CDS | 35.0% | |
| ! | AAGAAAGAAGTACGTTGAGT+TGG | + | chr5.3:75890341-75890360 | MS.gene63818:CDS | 35.0% |
| ! | TGAGCTAGAAGCTAAAGTTT+TGG | + | chr5.3:75890530-75890549 | MS.gene63818:CDS | 35.0% |
| !!! | AGAAGCTAAAGTTTTGGAGT+TGG | + | chr5.3:75890536-75890555 | MS.gene63818:CDS | 35.0% |
| AGAGAGGAACTTTGTCAGAA+AGG | + | chr5.3:75890153-75890172 | MS.gene63818:CDS | 40.0% | |
| ATGTCAAGAAAGCCAAAGTC+AGG | + | chr5.3:75890922-75890941 | MS.gene63818:CDS | 40.0% | |
| CACCATCTTCAATCACAAGT+GGG | - | chr5.3:75891013-75891032 | None:intergenic | 40.0% | |
| CGAGCTTCGAAGTAAATTGA+AGG | + | chr5.3:75890602-75890621 | MS.gene63818:CDS | 40.0% | |
| CTGTTGTAGTACTAAGAGAG+AGG | + | chr5.3:75890244-75890263 | MS.gene63818:CDS | 40.0% | |
| GAGAGGAACTTTGTCAGAAA+GGG | + | chr5.3:75890154-75890173 | MS.gene63818:CDS | 40.0% | |
| GCATGAAAGAAATGCAAGGT+TGG | + | chr5.3:75890713-75890732 | MS.gene63818:CDS | 40.0% | |
| TCACCATCTTCAATCACAAG+TGG | - | chr5.3:75891014-75891033 | None:intergenic | 40.0% | |
| TCTAACACTAGACCTGACTT+TGG | - | chr5.3:75890937-75890956 | None:intergenic | 40.0% | |
| TGAAAGAAATGCAAGGTTGG+AGG | + | chr5.3:75890716-75890735 | MS.gene63818:CDS | 40.0% | |
| TGATCATGTTGCAAATGCAC+CGG | + | chr5.3:75890758-75890777 | MS.gene63818:CDS | 40.0% | |
| TTCAGCAAGAATCACGTAGT+TGG | - | chr5.3:75889914-75889933 | None:intergenic | 40.0% | |
| TTTGTACCATCAGACACATG+AGG | - | chr5.3:75890792-75890811 | None:intergenic | 40.0% | |
| ! | AACAAGATGATTGATGGTCC+AGG | + | chr5.3:75890972-75890991 | MS.gene63818:CDS | 40.0% |
| ! | AGATGACCCATGTTCGATTT+TGG | - | chr5.3:75889887-75889906 | None:intergenic | 40.0% |
| ! | GATGACCCATGTTCGATTTT+GGG | - | chr5.3:75889886-75889905 | None:intergenic | 40.0% |
| ! | TGAGTTGAGGAAACTGTTTG+AGG | + | chr5.3:75890272-75890291 | MS.gene63818:CDS | 40.0% |
| ! | TGTGATTGAAGATGGTGATG+AGG | + | chr5.3:75891016-75891035 | MS.gene63818:CDS | 40.0% |
| !! | GAGGTTGATGGTATTGATGA+TGG | + | chr5.3:75891035-75891054 | MS.gene63818:CDS | 40.0% |
| AAGCAAAAAAGGGTGGAGGA+TGG | + | chr5.3:75890471-75890490 | MS.gene63818:CDS | 45.0% | |
| AGCAAAAAAGGGTGGAGGAT+GGG | + | chr5.3:75890472-75890491 | MS.gene63818:CDS | 45.0% | |
| AGCAAGAATCACGTAGTTGG+TGG | - | chr5.3:75889911-75889930 | None:intergenic | 45.0% | |
| AGCCCACTTGTGATTGAAGA+TGG | + | chr5.3:75891008-75891027 | MS.gene63818:CDS | 45.0% | |
| GGAGTTGAAGCAAAAAAGGG+TGG | + | chr5.3:75890464-75890483 | MS.gene63818:CDS | 45.0% | |
| GTTGAAGCAAAAAAGGGTGG+AGG | + | chr5.3:75890467-75890486 | MS.gene63818:CDS | 45.0% | |
| TCTTTGAACACGGAGTTCAG+AGG | + | chr5.3:75890687-75890706 | MS.gene63818:CDS | 45.0% | |
| ! | GAAGATGGTGATGAGGTTGA+TGG | + | chr5.3:75891023-75891042 | MS.gene63818:CDS | 45.0% |
| ! | GAGAGAGGAACATTGAGTTG+AGG | + | chr5.3:75890259-75890278 | MS.gene63818:CDS | 45.0% |
| GCAACACCTCATGTGTCTGA+TGG | + | chr5.3:75890783-75890802 | MS.gene63818:CDS | 50.0% | |
| GGAGGATGTTGAAGCAAGTC+AGG | + | chr5.3:75890734-75890753 | MS.gene63818:CDS | 50.0% | |
| !! | GACACATGAGGTGTTGCAAC+CGG | - | chr5.3:75890780-75890799 | None:intergenic | 50.0% |
| !! | GTACGTTGAGTTGGAGTGTG+AGG | + | chr5.3:75890350-75890369 | MS.gene63818:CDS | 50.0% |
| GGGCTGCTTGCATCTTTGCC+TGG | - | chr5.3:75890993-75891012 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75889871 | 75891070 | 75889871 | ID=MS.gene63818 |
| chr5.3 | mRNA | 75889871 | 75891070 | 75889871 | ID=MS.gene63818.t1;Parent=MS.gene63818 |
| chr5.3 | exon | 75889871 | 75890812 | 75889871 | ID=MS.gene63818.t1.exon1;Parent=MS.gene63818.t1 |
| chr5.3 | CDS | 75889871 | 75890812 | 75889871 | ID=cds.MS.gene63818.t1;Parent=MS.gene63818.t1 |
| chr5.3 | exon | 75890885 | 75891070 | 75890885 | ID=MS.gene63818.t1.exon2;Parent=MS.gene63818.t1 |
| chr5.3 | CDS | 75890885 | 75891070 | 75890885 | ID=cds.MS.gene63818.t1;Parent=MS.gene63818.t1 |
| Gene Sequence |
| Protein sequence |
