AlfalfaGEDB | MS.gene64516

M. sativa cultivar XinJiangDaYe / MS.gene64516

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64516.t1	XP_020212665.1	98.6	138	2	0	1	138	1	138	3.10E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64516.t1	Q1SU99	99.3	138	1	0	1	138	1	138	2.2e-43	176.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64516.t1	A0A151TUN5	98.6	138	2	0	1	138	1	138	2.3e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050689	MS.gene64516	0.801423	9.07E-49	-1.69E-46
MS.gene051328	MS.gene64516	0.801047	1.08E-48	-1.69E-46
MS.gene056215	MS.gene64516	0.809376	1.95E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene64516	MS.gene051008	PPI
MS.gene75219	MS.gene64516	PPI
MS.gene65007	MS.gene64516	PPI
MS.gene64516	MS.gene84498	PPI
MS.gene025426	MS.gene64516	PPI
MS.gene035396	MS.gene64516	PPI
MS.gene049824	MS.gene64516	PPI
MS.gene64516	MS.gene65249	PPI
MS.gene64516	MS.gene08465	PPI
MS.gene64516	MS.gene99525	PPI
MS.gene004127	MS.gene64516	PPI
MS.gene64516	MS.gene64653	PPI
MS.gene64516	MS.gene041623	PPI
MS.gene26950	MS.gene64516	PPI
MS.gene64516	MS.gene025035	PPI
MS.gene004126	MS.gene64516	PPI
MS.gene64516	MS.gene63143	PPI
MS.gene64516	MS.gene81051	PPI
MS.gene025425	MS.gene64516	PPI
MS.gene64516	MS.gene63953	PPI
MS.gene64516	MS.gene066300	PPI
MS.gene64516	MS.gene27326	PPI
MS.gene64516	MS.gene066301	PPI
MS.gene64516	MS.gene049822	PPI
MS.gene066348	MS.gene64516	PPI
MS.gene24295	MS.gene64516	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64516.t1	MTR_7g099960	98.551	138	2	0	1	138	1	138	1.01e-93	267
MS.gene64516.t1	MTR_4g063240	85.135	148	11	3	1	138	1	147	1.24e-80	234
MS.gene64516.t1	MTR_1g090780	92.806	139	7	3	1	138	1	137	3.35e-78	228
MS.gene64516.t1	MTR_1g492910	92.806	139	7	3	1	138	1	137	3.38e-78	228
MS.gene64516.t1	MTR_4g063220	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene64516.t1	MTR_4g063990	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene64516.t1	MTR_4g063200	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene64516.t1	MTR_4g064020	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene64516.t1	MTR_4g071180	85.906	149	9	3	1	138	1	148	7.00e-76	222
MS.gene64516.t1	MTR_4g070240	85.906	149	9	3	1	138	1	148	7.00e-76	222
MS.gene64516.t1	MTR_4g088025	86.806	144	12	3	1	138	1	143	1.82e-74	218
MS.gene64516.t1	MTR_4g070245	84.564	149	11	3	1	138	1	148	3.47e-74	218
MS.gene64516.t1	MTR_4g063275	83.221	149	13	3	1	138	1	148	1.11e-73	216
MS.gene64516.t1	MTR_4g064983	84.828	145	10	3	1	134	1	144	2.78e-72	216
MS.gene64516.t1	MTR_1g068600	95.370	108	4	1	31	138	30	136	7.95e-65	194
MS.gene64516.t1	MTR_2g084480	60.674	89	35	0	49	137	136	224	3.00e-37	127
MS.gene64516.t1	MTR_8g069990	71.910	89	22	2	9	97	3	88	2.53e-35	117
MS.gene64516.t1	MTR_4g063270	90.196	51	5	0	76	126	1	51	4.22e-27	95.9
MS.gene64516.t1	MTR_7g114580	90.244	41	4	0	98	138	11	51	6.81e-20	77.4
MS.gene64516.t1	MTR_4g032777	39.744	78	47	0	45	122	33	110	7.10e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64516.t1	AT2G37470	89.130	138	14	1	1	137	1	138	3.78e-78	228
MS.gene64516.t1	AT3G53650	89.130	138	15	0	1	138	1	138	6.07e-77	224
MS.gene64516.t1	AT3G45980	82.119	151	13	3	1	138	1	150	4.28e-74	218
MS.gene64516.t1	AT1G07790	81.879	149	15	2	1	138	1	148	3.71e-73	215
MS.gene64516.t1	AT2G28720	81.579	152	13	3	1	138	1	151	8.00e-73	214
MS.gene64516.t1	AT5G59910	85.185	135	18	1	6	138	16	150	6.42e-71	209
MS.gene64516.t1	AT3G46030	87.597	129	14	1	12	138	17	145	2.76e-70	208
MS.gene64516.t1	AT5G02570	84.397	141	10	3	1	138	1	132	3.15e-67	199
MS.gene64516.t1	AT5G22880	81.119	143	18	2	5	138	3	145	5.85e-66	197
MS.gene64516.t1	AT3G09480	82.114	123	21	1	16	138	5	126	1.50e-63	190
MS.gene64516.t1	AT1G08170	60.000	85	34	0	51	135	151	235	3.45e-37	127

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGAGCTGGTGAATT+TGG	0.235894	7.3:-12628053	None:intergenic
CTCCGCCTTTGGTTTCTTCT+CGG	0.278156	7.3:-12627717	None:intergenic
GCTGTTAGATTGGTTCTTCC+TGG	0.314946	7.3:+12627986	MS.gene64516:CDS
GAGATCTTCTTCTCCGCCTT+TGG	0.325858	7.3:-12627728	None:intergenic
GCCTTTGGTTTCTTCTCGGC+TGG	0.347622	7.3:-12627713	None:intergenic
ATTGGTTCTTCCTGGAGAAT+TGG	0.353160	7.3:+12627994	MS.gene64516:CDS
CATCTTCAAGGTTCTGAAAC+AGG	0.359264	7.3:+12627817	MS.gene64516:CDS
AAACAGGTTCATCCTGATAT+TGG	0.374570	7.3:+12627833	MS.gene64516:CDS
GATTCAAACTGCTGTTAGAT+TGG	0.401031	7.3:+12627976	MS.gene64516:CDS
TGCTCAGGAATCGTCTCGTT+TGG	0.412474	7.3:+12627916	MS.gene64516:CDS
TTGCTTGAAACACCAATATC+AGG	0.417581	7.3:-12627845	None:intergenic
TGGTGTTTCAAGCAAGGCTA+TGG	0.418611	7.3:+12627853	MS.gene64516:CDS
TCGGCTGGAACTTTCTCTGC+GGG	0.455951	7.3:-12627698	None:intergenic
TGCTGTTTCTGAAGGGACTA+AGG	0.469671	7.3:+12628024	MS.gene64516:CDS
ATACAAGATATACATCTTCA+AGG	0.488494	7.3:+12627805	MS.gene64516:CDS
GCTAAACATGCTGTTTCTGA+AGG	0.504101	7.3:+12628016	MS.gene64516:CDS
TCTGCGGGCTTCTTCTCCGC+GGG	0.504996	7.3:-12627683	None:intergenic
GCATGTTTAGCCAATTCTCC+AGG	0.514866	7.3:-12628004	None:intergenic
TATCTTTGAGAAACTTGCTC+AGG	0.528928	7.3:+12627901	MS.gene64516:CDS
ATCTCCCTTGAAGTGATCGT+CGG	0.552358	7.3:-12627956	None:intergenic
CTCTGCGGGCTTCTTCTCCG+CGG	0.552987	7.3:-12627684	None:intergenic
GGTGTTTCAAGCAAGGCTAT+GGG	0.553844	7.3:+12627854	MS.gene64516:CDS
AGAAGCCGACGATCACTTCA+AGG	0.560392	7.3:+12627951	MS.gene64516:CDS
GAGAAGAAGATCTCAAAAGA+AGG	0.561490	7.3:+12627737	MS.gene64516:CDS
TGATATTGGTGTTTCAAGCA+AGG	0.568075	7.3:+12627847	MS.gene64516:CDS
AGGAATCGTCTCGTTTGGCT+AGG	0.569875	7.3:+12627921	MS.gene64516:CDS
AAAGCAACAAAATCAAGAGC+TGG	0.570887	7.3:-12628062	None:intergenic
CTAAACATGCTGTTTCTGAA+GGG	0.585084	7.3:+12628017	MS.gene64516:CDS
CTCGGCTGGAACTTTCTCTG+CGG	0.596510	7.3:-12627699	None:intergenic
AAAGGCAGAAAAGAAACCCG+CGG	0.639354	7.3:+12627667	MS.gene64516:CDS
ATCAACAATGGCTCCCACAA+AGG	0.640475	7.3:+12627649	None:intergenic
GAAGCCGACGATCACTTCAA+GGG	0.644853	7.3:+12627952	MS.gene64516:CDS
TCCAGCCGAGAAGAAACCAA+AGG	0.673169	7.3:+12627712	MS.gene64516:CDS
AGCCGAGAAGAAACCAAAGG+CGG	0.691581	7.3:+12627715	MS.gene64516:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATACATCTTCA+AGG	+	chr7.3:12627805-12627824	MS.gene64516:CDS	25.0%
	AAACAGGTTCATCCTGATAT+TGG	+	chr7.3:12627833-12627852	MS.gene64516:CDS	35.0%
	GAGAAGAAGATCTCAAAAGA+AGG	+	chr7.3:12627737-12627756	MS.gene64516:CDS	35.0%
	GATTCAAACTGCTGTTAGAT+TGG	+	chr7.3:12627976-12627995	MS.gene64516:CDS	35.0%
	TTGCTTGAAACACCAATATC+AGG	-	chr7.3:12627848-12627867	None:intergenic	35.0%
!	TATCTTTGAGAAACTTGCTC+AGG	+	chr7.3:12627901-12627920	MS.gene64516:CDS	35.0%
!	TGATATTGGTGTTTCAAGCA+AGG	+	chr7.3:12627847-12627866	MS.gene64516:CDS	35.0%
!!	CTAAACATGCTGTTTCTGAA+GGG	+	chr7.3:12628017-12628036	MS.gene64516:CDS	35.0%
!	ATTGGTTCTTCCTGGAGAAT+TGG	+	chr7.3:12627994-12628013	MS.gene64516:CDS	40.0%
!	GGTTTCTTTTCTGCCTTTGT+GGG	-	chr7.3:12627665-12627684	None:intergenic	40.0%
!!	CATCTTCAAGGTTCTGAAAC+AGG	+	chr7.3:12627817-12627836	MS.gene64516:CDS	40.0%
!!	GCTAAACATGCTGTTTCTGA+AGG	+	chr7.3:12628016-12628035	MS.gene64516:CDS	40.0%
	AAAGGCAGAAAAGAAACCCG+CGG	+	chr7.3:12627667-12627686	MS.gene64516:CDS	45.0%
	ATCTCCCTTGAAGTGATCGT+CGG	-	chr7.3:12627959-12627978	None:intergenic	45.0%
	GCATGTTTAGCCAATTCTCC+AGG	-	chr7.3:12628007-12628026	None:intergenic	45.0%
	GGTGTTTCAAGCAAGGCTAT+GGG	+	chr7.3:12627854-12627873	MS.gene64516:CDS	45.0%
	TGGTGTTTCAAGCAAGGCTA+TGG	+	chr7.3:12627853-12627872	MS.gene64516:CDS	45.0%
!	GCTGTTAGATTGGTTCTTCC+TGG	+	chr7.3:12627986-12628005	MS.gene64516:CDS	45.0%
!	GGGTTTCTTTTCTGCCTTTG+TGG	-	chr7.3:12627666-12627685	None:intergenic	45.0%
!!	TGCTGTTTCTGAAGGGACTA+AGG	+	chr7.3:12628024-12628043	MS.gene64516:CDS	45.0%
	AGAAGCCGACGATCACTTCA+AGG	+	chr7.3:12627951-12627970	MS.gene64516:CDS	50.0%
	AGCCGAGAAGAAACCAAAGG+CGG	+	chr7.3:12627715-12627734	MS.gene64516:CDS	50.0%
	AGGAATCGTCTCGTTTGGCT+AGG	+	chr7.3:12627921-12627940	MS.gene64516:CDS	50.0%
	GAAGCCGACGATCACTTCAA+GGG	+	chr7.3:12627952-12627971	MS.gene64516:CDS	50.0%
	GAGATCTTCTTCTCCGCCTT+TGG	-	chr7.3:12627731-12627750	None:intergenic	50.0%
	TCCAGCCGAGAAGAAACCAA+AGG	+	chr7.3:12627712-12627731	MS.gene64516:CDS	50.0%
	TGCTCAGGAATCGTCTCGTT+TGG	+	chr7.3:12627916-12627935	MS.gene64516:CDS	50.0%
!	CTCCGCCTTTGGTTTCTTCT+CGG	-	chr7.3:12627720-12627739	None:intergenic	50.0%
	CTCGGCTGGAACTTTCTCTG+CGG	-	chr7.3:12627702-12627721	None:intergenic	55.0%
	TCGGCTGGAACTTTCTCTGC+GGG	-	chr7.3:12627701-12627720	None:intergenic	55.0%
!	GCCTTTGGTTTCTTCTCGGC+TGG	-	chr7.3:12627716-12627735	None:intergenic	55.0%
	CTCTGCGGGCTTCTTCTCCG+CGG	-	chr7.3:12627687-12627706	None:intergenic	65.0%
	TCTGCGGGCTTCTTCTCCGC+GGG	-	chr7.3:12627686-12627705	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	12627656	12628072	12627656	ID=MS.gene64516
chr7.3	mRNA	12627656	12628072	12627656	ID=MS.gene64516.t1;Parent=MS.gene64516
chr7.3	exon	12627656	12628072	12627656	ID=MS.gene64516.t1.exon1;Parent=MS.gene64516.t1
chr7.3	CDS	12627656	12628072	12627656	ID=cds.MS.gene64516.t1;Parent=MS.gene64516.t1

Gene Sequence

>MS.gene64516

ATGGCTCCCACAAAGGCAGAAAAGAAACCCGCGGAGAAGAAGCCCGCAGAGAAAGTTCCAGCCGAGAAGAAACCAAAGGCGGAGAAGAAGATCTCAAAAGAAGGAAGCAGCGACAAGAAGAAAAAGAGAACGAAGAAAAGCGTTGAAACATACAAGATATACATCTTCAAGGTTCTGAAACAGGTTCATCCTGATATTGGTGTTTCAAGCAAGGCTATGGGAATTATGAACAGTTTCATTAACGATATCTTTGAGAAACTTGCTCAGGAATCGTCTCGTTTGGCTAGGTATAACAAGAAGCCGACGATCACTTCAAGGGAGATTCAAACTGCTGTTAGATTGGTTCTTCCTGGAGAATTGGCTAAACATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAATTCACCAGCTCTTGA

Protein sequence

>MS.gene64516.t1

MAPTKAEKKPAEKKPAEKVPAEKKPKAEKKISKEGSSDKKKKRTKKSVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS