AlfalfaGEDB | MS.gene65297

M. sativa cultivar XinJiangDaYe / MS.gene65297

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65297.t1	RHN54425.1	91.7	530	20	2	1	508	26	553	7.70E-279	969.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65297.t1	F4HUK6	42.1	530	269	8	9	508	9	530	2.1e-115	417.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65297.t1	G7K007	91.7	530	20	2	1	508	1	528	5.6e-279	969.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050148	MS.gene65297	0.806805	6.88E-50	-1.69E-46
MS.gene050149	MS.gene65297	0.804255	2.36E-49	-1.69E-46
MS.gene050961	MS.gene65297	0.849948	2.33E-60	-1.69E-46
MS.gene051341	MS.gene65297	0.814251	1.69E-51	-1.69E-46
MS.gene05156	MS.gene65297	0.827101	1.88E-54	-1.69E-46
MS.gene051586	MS.gene65297	0.840792	7.02E-58	-1.69E-46
MS.gene052053	MS.gene65297	0.814804	1.28E-51	-1.69E-46
MS.gene052559	MS.gene65297	0.81969	1.02E-52	-1.69E-46
MS.gene052560	MS.gene65297	0.821433	4.05E-53	-1.69E-46
MS.gene053601	MS.gene65297	0.812111	5.00E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65297.t1	MTR_5g023730	91.698	530	20	2	1	508	1	528	0.0	979
MS.gene65297.t1	MTR_5g008800	46.053	532	252	9	3	508	2	524	1.14e-158	462
MS.gene65297.t1	MTR_5g008820	44.697	528	257	8	6	508	5	522	3.17e-154	451
MS.gene65297.t1	MTR_8g074040	44.757	534	258	10	3	508	44	568	5.34e-149	440
MS.gene65297.t1	MTR_8g074120	46.996	466	236	6	48	508	20	479	1.33e-140	415
MS.gene65297.t1	MTR_8g074040	45.733	457	233	7	59	508	2	450	4.11e-134	397
MS.gene65297.t1	MTR_4g121600	39.633	545	284	10	3	508	5	543	2.20e-129	388
MS.gene65297.t1	MTR_2g044180	38.574	547	291	10	1	508	3	543	4.92e-127	382
MS.gene65297.t1	MTR_5g076240	38.033	539	292	11	4	508	2	532	9.86e-121	365
MS.gene65297.t1	MTR_3g064420	38.333	540	286	11	4	504	3	534	1.51e-119	363

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65297.t1	AT1G20560	43.585	530	261	8	9	508	9	530	3.30e-151	444
MS.gene65297.t1	AT2G17650	43.446	534	254	11	15	508	60	585	5.48e-138	411
MS.gene65297.t1	AT1G20560	44.760	458	236	7	60	508	3	452	3.38e-135	400
MS.gene65297.t1	AT1G76290	40.075	534	279	10	3	508	2	522	9.96e-123	370
MS.gene65297.t1	AT3G16910	38.476	538	283	10	11	508	17	546	1.17e-122	371
MS.gene65297.t1	AT5G16340	39.074	540	283	11	3	508	2	529	1.79e-122	370
MS.gene65297.t1	AT1G66120	39.963	538	281	13	4	507	3	532	6.22e-122	369
MS.gene65297.t1	AT5G16370	38.519	540	286	11	3	508	2	529	1.79e-119	362
MS.gene65297.t1	AT1G75960	39.439	535	277	12	9	508	7	529	1.49e-117	357
MS.gene65297.t1	AT1G65890	36.464	543	299	9	4	506	2	538	7.11e-115	351

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Find 91 sgRNAs with CRISPR-Local

Find 369 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATATTATTTATTCTA+AGG	0.210086	5.2:+14592660	MS.gene65297:CDS
AAAGATATTATTTATTCTAA+GGG	0.478681	5.2:+14592661	MS.gene65297:CDS
AAAGGGAATACTTCAATGTC+AGG	0.397558	5.2:+14592526	MS.gene65297:CDS
AAATGAAGTTCATAGTGTGC+TGG	0.549199	5.2:-14591575	None:intergenic
AACAACATTAGCATTAATAT+TGG	0.350853	5.2:+14591652	MS.gene65297:CDS
AACATGATCATTGTAGATGA+TGG	0.453182	5.2:-14587141	None:intergenic
AACCTTGTCACCATACACAG+TGG	0.766651	5.2:-14587117	None:intergenic
AACCTTTAGAGCACTCAACC+AGG	0.592689	5.2:-14587052	None:intergenic
AACTACACATCAGGCTCAAC+TGG	0.349825	5.2:+14591917	MS.gene65297:CDS
AAGAAGTTCATCGTATATTA+AGG	0.246952	5.2:-14591831	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGAATGATGTCAGAAA+AGG	-	chr5.2:14589374-14589393	None:intergenic	30.0%
AAATAGTTAATAAGCTCCAC+AGG	+	chr5.2:14587673-14587692	MS.gene65297:intron	30.0%
AACATGATCATTGTAGATGA+TGG	-	chr5.2:14587144-14587163	None:intergenic	30.0%
AATAGACCATGTACAAGAAT+GGG	-	chr5.2:14589612-14589631	None:intergenic	30.0%
ACAACACATACAACACATTT+GGG	+	chr5.2:14589515-14589534	MS.gene65297:intron	30.0%
AGAATTATTTCAGATCCTGA+AGG	-	chr5.2:14589018-14589037	None:intergenic	30.0%
AGAGAGAATGAACTTTGTAA+AGG	-	chr5.2:14588819-14588838	None:intergenic	30.0%
AGATCAATTAGATGCTCATA+TGG	+	chr5.2:14592867-14592886	MS.gene65297:CDS	30.0%
AGTTTGCAATAATTTCCAGA+AGG	-	chr5.2:14592198-14592217	None:intergenic	30.0%
ATAAGGAGAAATCGAAAACA+AGG	-	chr5.2:14589635-14589654	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	14587041	14593342	14587041	ID=MS.gene65297
chr5.2	mRNA	14587041	14593342	14587041	ID=MS.gene65297.t1;Parent=MS.gene65297
chr5.2	exon	14587041	14587160	14587041	ID=MS.gene65297.t1.exon1;Parent=MS.gene65297.t1
chr5.2	CDS	14587041	14587160	14587041	ID=cds.MS.gene65297.t1;Parent=MS.gene65297.t1
chr5.2	exon	14587227	14587247	14587227	ID=MS.gene65297.t1.exon2;Parent=MS.gene65297.t1
chr5.2	CDS	14587227	14587247	14587227	ID=cds.MS.gene65297.t1;Parent=MS.gene65297.t1
chr5.2	exon	14591551	14592934	14591551	ID=MS.gene65297.t1.exon3;Parent=MS.gene65297.t1
chr5.2	CDS	14591551	14592934	14591551	ID=cds.MS.gene65297.t1;Parent=MS.gene65297.t1
chr5.2	exon	14593335	14593342	14593335	ID=MS.gene65297.t1.exon4;Parent=MS.gene65297.t1
chr5.2	CDS	14593335	14593342	14593335	ID=cds.MS.gene65297.t1;Parent=MS.gene65297.t1

Gene Sequence

>MS.gene65297

ATGACACAAGACCTGGTTGAGTGCTCTAAAGGTTCACACATACCTTTGACACCGATCAGTTTCCTAGAACGAGCAGCCACTGTGTATGGTGACAAGGTTTCCATCATCTACAATGATCATGTTAGATTTTCTTGGAGAGAAACCTATGAAAGATGTCTCAACCTTGCTTCTGCTTGGTCAACTTAGGAATTTCTAATGGTTGATATTGTAAGTTCATTTATCTCCTATCTCCCTATGATGACTACATTAATTCGTATACTTTCCTTTGCCCTGCTTTCATTTCATCTAAAGCCAACTATTGTTTGTTCCCTCTTCATGACCAAATATAATGTATCAATGTAATTTAAATTAGAAAATATATATGATCAATGTTTTATATCACTTGTAACAAAATATAAGCACAAAATCAGATCTAGAAGTCAAATGTTTTTGTCTAAATTTTGGACTAATTACTTTTAAATATTTTTTGCTTATATTTTGTTAGAGTTTGCCAATATTTTGTTACAGTTTACCAATAGAATTATGTTCTTCAATCGTGCATGAATTAATTAACTGGTTATTCTAATTGGTCCATACAAACTAGTGTTCTATCCATGGTTTTAAATTGGTCATTAAATCATACAATTAGGCTTAAATAGTTAATAAGCTCCACAGGAGGATGATTGAACTTGATTATTGGGTGAAATAATTTTGCCTAGACTTTAACTTACCTTATCACTGACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGCTTACCACTGAACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGGATTAACAAAAAAAAAAAAGTTCTTTGAATCGATTACACTAATCAATTTGGCAACCAAGCGACCAGTTTTGCAGTCACTTAATGAAAGTTTTTTAGCACGTAATTAGCAGTTAATTATGGAGAATGCTAAATAGTATTCCAGAGTATTGGTTATACACCCAAAGAAGTAAGTTTTTATTAAAAGTGGTATAATTATTACTTAATTAAAAGTAACATCTTATATTTTCAAAACAAAAATTCTATTTTTAAAATTACTTAACCAGTGCCCCAAAGACAAGACACCGATTAACAAGACCCTAATTATAAATATTAAATTTCATATTATAAGGATAATGCTAACCAGTACCCCAATGACATTGGTTAAGCATCCAAAACAAACAAGTTTTCATGAAAGAGTTGTGAGATCATTACTTGGTCAAAAATAACATCTTATATTTTCAAGATAAAATTTCTATTTATAGAATCCTTAACCAATACTCTGAGGACACTGGTTAGCAAGATCCTAATATAAATATTAATTTCACTGAATCAACGGTTGATTATTACAATATCATATAATGATCAAGCCGATAAACTTTCTTCTTTTAATATTAAAAATAAAGTATTCATTTAAATTATATCAAATACAACACAACTTTTATATCGTTAAAAATAAAAAGGATAAATCTGCAAACAAACTCACACCGTTCATATTAAAAATGTAAAATCTAAAAATATGTGTTTACACACACATTGATGACTCGTTCATTATAGTTTCATTCAAAATTCAATTTATATTTGTTATTGTGATTTAAAACATTCAAAAAAGGCTTTTATATATTGCCAAGGTTCTAACTACAGTGTGTATTTTTTATGTATCAGAATCATTTTATGTAATGTGAATCTTTTTAAACTCTTTTAATGATAAAAATTTAATCCAAATTTGTAAAATTATATCGTTACGAAAAGGCCATCGAATTCAAAAAGCCTTTACAAAGTTCATTCTCTCTAAAAACTCTAACATGTCTCAAACGTGTGATTTTCGAGTCTAAAACTTATCGTTTACCAACTCTAACATGGCTCAAACGTGTGGACTATCGAGTCTAAAACTTATCGTTTATCAATAGACCAAGTATTTGGAGTTAGAAGATGATTCATTGGAAGTGAATGATTTCATATTTTTTTTCCTTCAGGATCTGAAATAATTCTATAAGCGCGTAATTATGGAAAATGGGACAGTACATGTATACCGTGTTCAACACTGTTTATCTATATTCATATATAGATATTTTTTTTGGTCAAGTCTATATCATATAATTTTAAAATGATTAATTCGCTGTCCTTGTTCTAAGACCGAGTTAGGTCATTGACATTTCATTCCATACCAGAATTTACGCCATTAAGAAAAAACATGTTATTAGAATCCCTTAAAAAAAAACACGTTATTAAATGGAAAGTCCTGTCTTTGAAAAACTTTATTTAAGCCATTAGAATGTCTTCAATATGGGAAGTCCACACAGATTTATTATTATTTTTTACTATCCTTTTCTGACATCATTCTGTTTGGAAGAGTATGATCTCACATTACAACACACACCATTCTGGGAAGCACTTGGGTGACATAATGTCACCGTGACATTAAATCTGAGCCACACAACACACATTGTCACTCAATATCTGGGCCACACAACACATACAACACATTTGGGCTACACAACACAACACCTTTGTGTGGCCCAGATTTAATGGCACGGTGACATTATGTCACCCAAGTATTTTCCCCATTCTTGTACATGGTCTATTCCTTGTTTTCGATTTCTCCTTATTTTCATTCTCTTCTGGAATTCACTACAGAATTGTTACACTATAGTAGATTGGTGTGGAATATGAATCTCTAAGAATGTAAACAAATTAAAAACTAAGGGTGTTACGTTAGCAAACTCTTTTTTTTTTTTTTTTTTGTTTTTTTGGACAAGTCAATATTTGTTATGATGATGTATCTATATATAATAAGAGTAAATTACATTTCTATTCCCTCAAAGATGTTTGAATTACATTACCTTTCCTTTTTTGTTAAAATATACACTCCTCTCACCTCTCATTCAAAATATATTAGAAAATATTTCACTCCCCACCCCTAAGAGAAACTTGAAATACATTCTCCTCCCCTCTTATGTTAAAATCTACACTTTAATCTCTCAATAATTTTTTTTATTTCTAATAATCTAGAAAAAAAATAATCTAACACACTTCGGAAAAAAAATAGTATTGCAGGTCTATTTTAATATAATCAAAATTTGAATACATATTTTGCAATTTTTTATTCACAATTTCATTAACATGTAATTTTAAAGTCTAATAAAAAAATATGAAACAAGCCAAGAAAAGCCTAATAAAAAATATGAAACAAGCGAAGATAAAATTAAAATATGTGAAAAATTATAAAATACAATAAATTATGGTTATTTAAAAATTAATTTTATACTCTAAAATATAAAATTAATAGCAAAATATTTAAATTTAATTATCATTGATATTTAAATTATAAAGAAATGAATTTATTTTTTTTAAATGGTGGTTCAAAATTAGTATATATCATAAAAATATTTATTTAAGTTAAAATATATTAAAGTGTAGTGCATATTTAATTATTAAGGGAGAGGGTTGTAATTCAAGCATCTTATAGGGGAAGGGAGTATAAATTTTTCTTTTCAAGGAAGGGGAGTGCATATTTTAACATAAGAGGGGAGATGAGTGTAATTCAAACATCTTTGAGGGAAGGGGAGTGTAACTTACTCTATATAATATCTACATTTGATTACTTTTATTTTTTTTGAAAAGATCTACATTTGATTTGTTAACTAACAATTGATACTACTTACTTTATTCTATTATCAAATAACAACATATCTGTCATTGTGAACTTAGCTCAGTTGGTATGGACAATGCATGATATAATATATAATATATGCAAGGTCCGGGGTTCAAACCCCGGCCACCACCAATAAAAAAATTGTTCCGGGGTTCAAACCCCGGCCACCACCAATAAAAAAATAACAACATATCTATTTTTTTTAAAAAAGTATATATCTATCATATATCTTTCTTTTTAAACAACCATATTCATTGCATTATCTTTCTTTTTAAACAACCATATTCATTGCATTTCGGTTAATATAGAATTCAAAATACAAGGAGGAATATAATAAAAAACTTGGCGACAAACAAATAATCAAGATAACCTAGTCAAAACATGAAGCACACCATTAGGTTTTCTATCAAAGTCAATATATTTTTTTTATTAGAAAGTCAATATTATATAAAACTAGCTATATGATCCTCCATTTGATTATTTACAAAAGTTTCTAAACAAATGCAACATGAGAATACAAATTAATATAGTGGTCTAGCAAAAACACTAAAATGTAGCTTGTCTAATTTCGGTTTATCCCCTCCCACATCATTTGTTTTGTACGGTCATATGCCACAAATGTCTAAGAGAAGAATATTTAAATTATAAGGTTGGAATTCCAAATTTTTCAAAGGGGACAAAAATGCATTTAACCTTTTAATTTATGTTTTGATTAATGAATTAACATTGTCATCAACTCTATTTTCCAAATAAATTTACCTAACCAACATGTAATTGCTCATTATAACCGAAAAGAATTTTTTTCCTATACATATGAAGACCTCAATTTTCTTTCTTATATTATAAACAGGTTGCAACTTTGGCACCAAATACTCCAGCACACTATGAACTTCATTTTGGTGTACCAATGGCAGGTGCTATTATTTCTTCACTTAACCTCAAACTAGATGCAACAACATTAGCATTAATATTGGAACAGTTAGAGTCTTGCAAAATCATATTTGTAGACTATGAGTTTATTGATTCTGTACTCAAAGCATCAAAAATAATTTCCCAAAGAAATTTCAAGCCACCCTTTATTGTCCTAATACAAGATCATGGCCAACTAGTGAAAGATATACCTCAAGATACCTTAATATACGATGAACTTCTTGAAAAGGGACAAAAAGATTTTAAGATTTCAATGCCTAGAAATGAATGTGATCCTATATCAGTGAACTACACATCAGGCTCAACTGGAATTCCAAAAGGAGCAGTTTATAATCATAGAAGTGTCTACCTTAATTCACTCGCGACTATTACTCGTTTCGATGTGAATCCTATGCTGGTGTTTTTATGGACAGTTGACATGTTTAGATGCAATGGATGGTGTTTCATTTGGTTAATGCCTGCTCTTGGTGGCACAAACATTTGTCTTAGAAATGATTTTTCAGCAAAAGATATTTTTGAAGCAATTCATGTTCATAAGGTAACACATTTGTGTGGTGCACCTACCCTTCTGGAAATTATTGCAAACTGTGACATTATTAGGCATTTTTCTCATAAGGTGAGTGTCACAGTTGCTGGTATATTACCATCATTCAAAATTCTTAACAAAGTGGCAGAGCTTGGATTTGATGTTAATATTGGTTATGGTATGACTGAAACATTAGGTCCAGTTATTGTAAGAAAGTGGAAACAAAACTTTGATGATGATATTACAAAGCTGAATTATGTTGATCAAAAAGGGGTTATTGACTTTATGATGGTTGAAGTTGATGTGAAAGATCCAAATACAATGAAGAGTGTTCCATATGATGGAAAAACCATAGGTGAAATCATGTTTAAAGGGAATACTTCAATGTCAGGGTATCTTAAGAGTTCACAAGTGAGTCATGAAGCTTTTAGGGATGGTTGGTATAGGACTAGGGACCTAGGTGTTAGGTTACCTAATGGTTCTTTCAATTTGAAAGATAGAGCAAAAGATATTATTTATTCTAAGGGTGAGTTTGTGAGTTCATTAGAGGTGGAAGCTGTTTTGTTGAATCATCCAATGGTTTTGAAAGTAGCTGTTGTGGGGAGATATGATGATGATGGTGACGAGTGTTTGGTTGAGTCACCTTGTGCAATTGTGAAATTGAAAGATGGATGTAGTGCTAATGTTGAAGATATTATCAAGTTTTGTGAAGATCAATTAGATGCTCATATGGTTCCTAAGAGTGTGGTGTTTGGTGATTTGCCTCTTAATTCAACTGGTAAGGTACAAAAGTTTGTTATTAGAGAAAATATTAAAAGTATTGGGTGGTGTGACTCAATTAATTAAGGCGTGGTTTGTAGGATTTGGATTGATTGCATAACAAATTTTACATATTCATGCAGTCCACATACATCGGTGATGGTTGAATCAAAATCGGACAACCCATATATCTAGCTTAATATTTTAGAGTTTAAAAATTTCAAAATAGATTGCTTAATGCACCATTTAATCTCTAATCTAATTATCACCGATATGCAGACTGCATGATTGTGCGAATTTATTACTGCAGCAAAGCCAAATCAGTGGTTTGCATGATAATAAATAAAACAAGGCAAAATGGAATGTGCCAAACAAGCATGTAATAATCTTAAATAAACTTATTTGTTGTTTTATTGTAGACACTTGA

Protein sequence

>MS.gene65297.t1

MTQDLVECSKGSHIPLTPISFLERAATVYGDKVSIIYNDHEFLMVDIVATLAPNTPAHYELHFGVPMAGAIISSLNLKLDATTLALILEQLESCKIIFVDYEFIDSVLKASKIISQRNFKPPFIVLIQDHGQLVKDIPQDTLIYDELLEKGQKDFKISMPRNECDPISVNYTSGSTGIPKGAVYNHRSVYLNSLATITRFDVNPMLVFLWTVDMFRCNGWCFIWLMPALGGTNICLRNDFSAKDIFEAIHVHKVTHLCGAPTLLEIIANCDIIRHFSHKVSVTVAGILPSFKILNKVAELGFDVNIGYGMTETLGPVIVRKWKQNFDDDITKLNYVDQKGVIDFMMVEVDVKDPNTMKSVPYDGKTIGEIMFKGNTSMSGYLKSSQVSHEAFRDGWYRTRDLGVRLPNGSFNLKDRAKDIIYSKGEFVSSLEVEAVLLNHPMVLKVAVVGRYDDDGDECLVESPCAIVKLKDGCSANVEDIIKFCEDQLDAHMVPKSVVFGDLPLNSTDT