Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67598.t1 | XP_003612270.1 | 97.6 | 127 | 3 | 0 | 1 | 127 | 59 | 185 | 3.20E-64 | 254.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67598.t1 | Q38853 | 63.7 | 124 | 44 | 1 | 5 | 127 | 59 | 182 | 7.2e-41 | 167.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67598.t1 | B7FGV4 | 97.6 | 127 | 3 | 0 | 1 | 127 | 59 | 185 | 2.3e-64 | 254.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene67598 | 0.861214 | 1.20E-63 | -1.69E-46 |
| MS.gene050406 | MS.gene67598 | 0.85751 | 1.55E-62 | -1.69E-46 |
| MS.gene052188 | MS.gene67598 | 0.809081 | 2.26E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene67598 | 0.828477 | 8.80E-55 | -1.69E-46 |
| MS.gene053073 | MS.gene67598 | 0.814942 | 1.19E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene67598 | 0.809113 | 2.22E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene67598 | 0.817538 | 3.13E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene67598 | 0.817187 | 3.76E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene67598 | 0.805233 | 1.47E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene67598 | 0.807021 | 6.20E-50 | -1.69E-46 |
| MS.gene056719 | MS.gene67598 | 0.813629 | 2.32E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene67598 | 0.833303 | 5.76E-56 | -1.69E-46 |
| MS.gene057396 | MS.gene67598 | 0.828651 | 7.98E-55 | -1.69E-46 |
| MS.gene058082 | MS.gene67598 | 0.822473 | 2.33E-53 | -1.69E-46 |
| MS.gene058748 | MS.gene67598 | 0.800116 | 1.68E-48 | -1.69E-46 |
| MS.gene059692 | MS.gene67598 | 0.805481 | 1.31E-49 | -1.69E-46 |
| MS.gene059695 | MS.gene67598 | 0.802878 | 4.55E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene67598 | 0.822997 | 1.76E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene67598 | 0.832858 | 7.44E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67598.t1 | MTR_5g023170 | 97.638 | 127 | 3 | 0 | 1 | 127 | 59 | 185 | 1.25e-91 | 263 |
| MS.gene67598.t1 | MTR_5g023170 | 98.058 | 103 | 2 | 0 | 1 | 103 | 59 | 161 | 2.90e-73 | 216 |
| MS.gene67598.t1 | MTR_5g022390 | 49.123 | 114 | 56 | 1 | 16 | 127 | 31 | 144 | 2.16e-32 | 111 |
| MS.gene67598.t1 | MTR_2g017630 | 44.037 | 109 | 60 | 1 | 17 | 124 | 12 | 120 | 1.38e-29 | 104 |
| MS.gene67598.t1 | MTR_8g075420 | 45.833 | 120 | 59 | 2 | 3 | 120 | 5 | 120 | 1.54e-28 | 101 |
| MS.gene67598.t1 | MTR_2g059890 | 40.164 | 122 | 68 | 2 | 4 | 124 | 3 | 120 | 2.12e-28 | 101 |
| MS.gene67598.t1 | MTR_8g075420 | 48.039 | 102 | 47 | 2 | 3 | 102 | 5 | 102 | 7.01e-23 | 86.3 |
| MS.gene67598.t1 | MTR_2g059890 | 40.404 | 99 | 54 | 2 | 3 | 100 | 2 | 96 | 1.27e-20 | 80.5 |
| MS.gene67598.t1 | MTR_2g059890 | 40.404 | 99 | 54 | 2 | 3 | 100 | 2 | 96 | 1.51e-20 | 80.5 |
| MS.gene67598.t1 | MTR_2g059890 | 40.816 | 98 | 53 | 2 | 4 | 100 | 3 | 96 | 4.83e-20 | 80.9 |
| MS.gene67598.t1 | MTR_5g022390 | 50.000 | 72 | 34 | 1 | 16 | 85 | 31 | 102 | 2.27e-16 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67598.t1 | AT4G35770 | 63.710 | 124 | 44 | 1 | 5 | 127 | 59 | 182 | 2.35e-56 | 173 |
| MS.gene67598.t1 | AT5G66040 | 65.487 | 113 | 39 | 0 | 14 | 126 | 7 | 119 | 8.35e-52 | 160 |
| MS.gene67598.t1 | AT4G35770 | 63.000 | 100 | 36 | 1 | 5 | 103 | 59 | 158 | 1.53e-42 | 137 |
| MS.gene67598.t1 | AT4G35770 | 63.000 | 100 | 36 | 1 | 5 | 103 | 59 | 158 | 3.26e-42 | 137 |
| MS.gene67598.t1 | AT4G35770 | 63.415 | 82 | 29 | 1 | 5 | 85 | 59 | 140 | 1.18e-33 | 115 |
| MS.gene67598.t1 | AT5G66170 | 47.009 | 117 | 56 | 2 | 9 | 123 | 12 | 124 | 2.22e-31 | 108 |
| MS.gene67598.t1 | AT5G66170 | 46.218 | 119 | 56 | 3 | 9 | 123 | 12 | 126 | 1.97e-29 | 103 |
| MS.gene67598.t1 | AT2G21045 | 41.441 | 111 | 65 | 0 | 17 | 127 | 43 | 153 | 2.19e-27 | 99.8 |
| MS.gene67598.t1 | AT5G66170 | 50.515 | 97 | 42 | 2 | 9 | 103 | 12 | 104 | 9.50e-25 | 92.0 |
| MS.gene67598.t1 | AT5G66040 | 60.938 | 64 | 25 | 0 | 63 | 126 | 1 | 64 | 1.10e-23 | 87.0 |
| MS.gene67598.t1 | AT2G17850 | 40.385 | 104 | 60 | 1 | 17 | 118 | 34 | 137 | 1.82e-22 | 86.7 |
| MS.gene67598.t1 | AT4G35770 | 63.333 | 60 | 21 | 1 | 5 | 63 | 59 | 118 | 2.64e-21 | 82.8 |
| MS.gene67598.t1 | AT4G35770 | 63.333 | 60 | 21 | 1 | 5 | 63 | 59 | 118 | 2.64e-21 | 82.8 |
| MS.gene67598.t1 | AT2G17850 | 31.933 | 119 | 70 | 2 | 19 | 126 | 59 | 177 | 5.54e-17 | 73.2 |
| MS.gene67598.t1 | AT2G17850 | 39.326 | 89 | 52 | 1 | 17 | 103 | 34 | 122 | 7.33e-16 | 68.9 |
Find 28 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGCTGGCCATAAGTATT+TGG | 0.181385 | 5.2:+14501858 | MS.gene67598:CDS |
| ACCTTACATCCAAATACTTA+TGG | 0.228445 | 5.2:-14501867 | None:intergenic |
| GTGTGGTTTAAAGGGGAATT+TGG | 0.241136 | 5.2:+14501780 | MS.gene67598:CDS |
| AAGCACGATGTGTGGTTTAA+AGG | 0.244947 | 5.2:+14501771 | None:intergenic |
| AGCACGATGTGTGGTTTAAA+GGG | 0.328888 | 5.2:+14501772 | None:intergenic |
| GATCCAACTTTGTACATATA+AGG | 0.335693 | 5.2:-14502223 | None:intergenic |
| TATGCTGCTTGGACTCAAAA+TGG | 0.348576 | 5.2:+14503111 | MS.gene67598:CDS |
| GGGAATTTGGAGGAAGTTGT+TGG | 0.404121 | 5.2:+14501793 | MS.gene67598:CDS |
| GCCATAAGTATTTGGATGTA+AGG | 0.427420 | 5.2:+14501866 | MS.gene67598:CDS |
| TAGCTGGTGGTTATGCTGCT+TGG | 0.436274 | 5.2:+14503100 | MS.gene67598:CDS |
| ACACGCACTGGCACTGAAGT+TGG | 0.437130 | 5.2:-14501820 | None:intergenic |
| ACCAGCTATGTCTGTGAGTC+CGG | 0.440393 | 5.2:-14503085 | None:intergenic |
| TATATGTACAAAGTTGGATC+AGG | 0.472445 | 5.2:+14502226 | MS.gene67598:CDS |
| TTTAATGCTGGACACGCTCC+TGG | 0.480736 | 5.2:+14502187 | MS.gene67598:CDS |
| ATACCTTATATGTACAAAGT+TGG | 0.502957 | 5.2:+14502220 | MS.gene67598:CDS |
| TGCATTGTATAGGGGTTCAC+CGG | 0.511879 | 5.2:+14503066 | MS.gene67598:intron |
| GCTCACGAGCTTCTTCTAGC+TGG | 0.514761 | 5.2:+14501844 | MS.gene67598:CDS |
| AGGGTTGTCAGCTTGGGAAA+AGG | 0.515357 | 5.2:+14502661 | MS.gene67598:intron |
| AGCTCGTGAGCTACACGCAC+TGG | 0.517304 | 5.2:-14501832 | None:intergenic |
| ACCACGGAAGAGTTTAATGC+TGG | 0.548422 | 5.2:+14502175 | MS.gene67598:CDS |
| TGGTTTAAAGGGGAATTTGG+AGG | 0.552343 | 5.2:+14501783 | MS.gene67598:CDS |
| GCTTGGGAAAAGGTCTATGA+TGG | 0.555495 | 5.2:+14502671 | MS.gene67598:CDS |
| ACCGGACTCACAGACATAGC+TGG | 0.570324 | 5.2:+14503084 | MS.gene67598:CDS |
| TAAGGTATGTTAATCGCACC+AGG | 0.610995 | 5.2:-14502205 | None:intergenic |
| GCACGATGTGTGGTTTAAAG+GGG | 0.620601 | 5.2:+14501773 | None:intergenic |
| ATTGTGATTGTGCAGGACCA+CGG | 0.621639 | 5.2:+14502159 | MS.gene67598:intron |
| TCCAGCATTAAACTCTTCCG+TGG | 0.640037 | 5.2:-14502176 | None:intergenic |
| GGACTCACAGACATAGCTGG+TGG | 0.717723 | 5.2:+14503087 | MS.gene67598:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGAATAGATTAGAAAAA+AGG | - | chr5.2:14502439-14502458 | None:intergenic | 15.0% |
| !! | CAAAACATTAGTATATTAAT+CGG | - | chr5.2:14502090-14502109 | None:intergenic | 15.0% |
| !! | AAATAACATACAAAATCTCA+TGG | - | chr5.2:14502596-14502615 | None:intergenic | 20.0% |
| !! | TAAAGTAACCAATTTAATCA+AGG | + | chr5.2:14501889-14501908 | MS.gene67598:intron | 20.0% |
| !! | TAAGAGATATATAATACTCT+TGG | + | chr5.2:14502328-14502347 | MS.gene67598:intron | 20.0% |
| !! | TATTTAAGCCTTGATTAAAT+TGG | - | chr5.2:14501900-14501919 | None:intergenic | 20.0% |
| !! | TTGTAATATTCTTAGTGTTT+AGG | + | chr5.2:14502277-14502296 | MS.gene67598:intron | 20.0% |
| !!! | AGTTGTTTTGATTAATCTAA+TGG | - | chr5.2:14502354-14502373 | None:intergenic | 20.0% |
| !!! | CATAATAGTTTTGTAGTTTT+AGG | - | chr5.2:14501963-14501982 | None:intergenic | 20.0% |
| !!! | CGATTAATATACTAATGTTT+TGG | + | chr5.2:14502088-14502107 | MS.gene67598:intron | 20.0% |
| !!! | TTAACTTCTTGAAAATTTTG+AGG | + | chr5.2:14503016-14503035 | MS.gene67598:intron | 20.0% |
| !!! | TTATTTTTGTTTACACTTTC+AGG | - | chr5.2:14502924-14502943 | None:intergenic | 20.0% |
| !!! | TTTTCATCAATAATTAGTCT+TGG | + | chr5.2:14502956-14502975 | MS.gene67598:intron | 20.0% |
| ! | AGATCAAAAAGAGATCAAAT+TGG | + | chr5.2:14502399-14502418 | MS.gene67598:intron | 25.0% |
| ! | ATACCTTATATGTACAAAGT+TGG | + | chr5.2:14502220-14502239 | MS.gene67598:CDS | 25.0% |
| ! | ATTAGAAAAAAGGACACTAA+TGG | - | chr5.2:14502429-14502448 | None:intergenic | 25.0% |
| ! | CATTAGCAGATGAATTAATT+TGG | - | chr5.2:14502628-14502647 | None:intergenic | 25.0% |
| ! | TAGATTAATCAAAACAACTG+AGG | + | chr5.2:14502355-14502374 | MS.gene67598:intron | 25.0% |
| ! | TATTGATGAAAAAAGTAGCA+CGG | - | chr5.2:14502948-14502967 | None:intergenic | 25.0% |
| !! | GTTAATAAAGTATCTATCAC+TGG | - | chr5.2:14503001-14503020 | None:intergenic | 25.0% |
| !!! | AATGTTTTGGAATTGTGATT+TGG | + | chr5.2:14502101-14502120 | MS.gene67598:intron | 25.0% |
| !!! | AATTGTTTTGCATTGTATAG+GGG | + | chr5.2:14503058-14503077 | MS.gene67598:intron | 25.0% |
| !!! | ACAATTGTTTTGCATTGTAT+AGG | + | chr5.2:14503056-14503075 | MS.gene67598:intron | 25.0% |
| !!! | CAATTGTTTTGCATTGTATA+GGG | + | chr5.2:14503057-14503076 | MS.gene67598:intron | 25.0% |
| AAACTCAAACAAGAAGTGAA+AGG | - | chr5.2:14502863-14502882 | None:intergenic | 30.0% | |
| AACTCAAACAAGAAGTGAAA+GGG | - | chr5.2:14502862-14502881 | None:intergenic | 30.0% | |
| AAGTATCTATCACTGGAAAT+TGG | - | chr5.2:14502994-14503013 | None:intergenic | 30.0% | |
| AATTATGTGTTCATGCATAC+AGG | + | chr5.2:14502477-14502496 | MS.gene67598:intron | 30.0% | |
| ACAATCACAATAAACAGGTA+TGG | - | chr5.2:14502150-14502169 | None:intergenic | 30.0% | |
| ACCTTACATCCAAATACTTA+TGG | - | chr5.2:14501870-14501889 | None:intergenic | 30.0% | |
| ATTATGTGTTCATGCATACA+GGG | + | chr5.2:14502478-14502497 | MS.gene67598:intron | 30.0% | |
| GATCCAACTTTGTACATATA+AGG | - | chr5.2:14502226-14502245 | None:intergenic | 30.0% | |
| TATATGTACAAAGTTGGATC+AGG | + | chr5.2:14502226-14502245 | MS.gene67598:CDS | 30.0% | |
| ! | ATCTTCTTTTCTAAACTGTG+AGG | - | chr5.2:14502538-14502557 | None:intergenic | 30.0% |
| ! | GAACAGCAATTTTGTTAAAG+AGG | + | chr5.2:14502509-14502528 | MS.gene67598:CDS | 30.0% |
| !! | CTAATGTTATTGCTTTTGCA+GGG | + | chr5.2:14502642-14502661 | MS.gene67598:intron | 30.0% |
| ACTCAAACAAGAAGTGAAAG+GGG | - | chr5.2:14502861-14502880 | None:intergenic | 35.0% | |
| CAACTGAGGTTTGATAGAAT+AGG | + | chr5.2:14502369-14502388 | MS.gene67598:intron | 35.0% | |
| GCCATAAGTATTTGGATGTA+AGG | + | chr5.2:14501866-14501885 | MS.gene67598:CDS | 35.0% | |
| TAGCACTCAACTAAAATGCT+AGG | - | chr5.2:14502889-14502908 | None:intergenic | 35.0% | |
| !! | GCTAATGTTATTGCTTTTGC+AGG | + | chr5.2:14502641-14502660 | MS.gene67598:intron | 35.0% |
| !!! | TAGCATTTTAGTTGAGTGCT+AGG | + | chr5.2:14502888-14502907 | MS.gene67598:intron | 35.0% |
| !!! | TTTTGAGGTCAAAGCTTGTT+TGG | + | chr5.2:14503031-14503050 | MS.gene67598:intron | 35.0% |
| ACATACAAAATCTCATGGCG+CGG | - | chr5.2:14502591-14502610 | None:intergenic | 40.0% | |
| CCTGCACAATCACAATAAAC+AGG | - | chr5.2:14502155-14502174 | None:intergenic | 40.0% | |
| CCTGTTTATTGTGATTGTGC+AGG | + | chr5.2:14502152-14502171 | MS.gene67598:intron | 40.0% | |
| CTCAAACAAGAAGTGAAAGG+GGG | - | chr5.2:14502860-14502879 | None:intergenic | 40.0% | |
| GTGTGGTTTAAAGGGGAATT+TGG | + | chr5.2:14501780-14501799 | MS.gene67598:CDS | 40.0% | |
| TAAGGTATGTTAATCGCACC+AGG | - | chr5.2:14502208-14502227 | None:intergenic | 40.0% | |
| TCTAGCTGGCCATAAGTATT+TGG | + | chr5.2:14501858-14501877 | MS.gene67598:CDS | 40.0% | |
| TGGTTTAAAGGGGAATTTGG+AGG | + | chr5.2:14501783-14501802 | MS.gene67598:CDS | 40.0% | |
| ! | GTATGGAAAGTCGTGAGTTT+TGG | - | chr5.2:14502133-14502152 | None:intergenic | 40.0% |
| ! | TATGCTGCTTGGACTCAAAA+TGG | + | chr5.2:14503111-14503130 | MS.gene67598:CDS | 40.0% |
| ACCACGGAAGAGTTTAATGC+TGG | + | chr5.2:14502175-14502194 | MS.gene67598:CDS | 45.0% | |
| ATTGTGATTGTGCAGGACCA+CGG | + | chr5.2:14502159-14502178 | MS.gene67598:intron | 45.0% | |
| GCTTGGGAAAAGGTCTATGA+TGG | + | chr5.2:14502671-14502690 | MS.gene67598:CDS | 45.0% | |
| GGGAATTTGGAGGAAGTTGT+TGG | + | chr5.2:14501793-14501812 | MS.gene67598:CDS | 45.0% | |
| TCCAGCATTAAACTCTTCCG+TGG | - | chr5.2:14502179-14502198 | None:intergenic | 45.0% | |
| TGCATTGTATAGGGGTTCAC+CGG | + | chr5.2:14503066-14503085 | MS.gene67598:intron | 45.0% | |
| TGGACTGAAAGTCGAGTACA+CGG | + | chr5.2:14501999-14502018 | MS.gene67598:intron | 45.0% | |
| ! | TTTTGCAGGGTTGTCAGCTT+GGG | + | chr5.2:14502655-14502674 | MS.gene67598:intron | 45.0% |
| !!! | TATGTAATTTTTAAATTTAT+TGG | + | chr5.2:14501979-14501998 | MS.gene67598:intron | 5.0% |
| ACCAGCTATGTCTGTGAGTC+CGG | - | chr5.2:14503088-14503107 | None:intergenic | 50.0% | |
| AGGGTTGTCAGCTTGGGAAA+AGG | + | chr5.2:14502661-14502680 | MS.gene67598:intron | 50.0% | |
| TAGCTGGTGGTTATGCTGCT+TGG | + | chr5.2:14503100-14503119 | MS.gene67598:CDS | 50.0% | |
| TTTAATGCTGGACACGCTCC+TGG | + | chr5.2:14502187-14502206 | MS.gene67598:CDS | 50.0% | |
| ! | CTTTTGCAGGGTTGTCAGCT+TGG | + | chr5.2:14502654-14502673 | MS.gene67598:intron | 50.0% |
| AAAATCTCATGGCGCGGCGA+AGG | - | chr5.2:14502585-14502604 | None:intergenic | 55.0% | |
| ACCGGACTCACAGACATAGC+TGG | + | chr5.2:14503084-14503103 | MS.gene67598:CDS | 55.0% | |
| GCTCACGAGCTTCTTCTAGC+TGG | + | chr5.2:14501844-14501863 | MS.gene67598:CDS | 55.0% | |
| GGACTCACAGACATAGCTGG+TGG | + | chr5.2:14503087-14503106 | MS.gene67598:CDS | 55.0% | |
| ! | ACACGCACTGGCACTGAAGT+TGG | - | chr5.2:14501823-14501842 | None:intergenic | 55.0% |
| AGCTCGTGAGCTACACGCAC+TGG | - | chr5.2:14501835-14501854 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 14501778 | 14503152 | 14501778 | ID=MS.gene67598 |
| chr5.2 | mRNA | 14501778 | 14503152 | 14501778 | ID=MS.gene67598.t1;Parent=MS.gene67598 |
| chr5.2 | exon | 14501778 | 14501887 | 14501778 | ID=MS.gene67598.t1.exon1;Parent=MS.gene67598.t1 |
| chr5.2 | CDS | 14501778 | 14501887 | 14501778 | ID=cds.MS.gene67598.t1;Parent=MS.gene67598.t1 |
| chr5.2 | exon | 14502174 | 14502247 | 14502174 | ID=MS.gene67598.t1.exon2;Parent=MS.gene67598.t1 |
| chr5.2 | CDS | 14502174 | 14502247 | 14502174 | ID=cds.MS.gene67598.t1;Parent=MS.gene67598.t1 |
| chr5.2 | exon | 14502499 | 14502569 | 14502499 | ID=MS.gene67598.t1.exon3;Parent=MS.gene67598.t1 |
| chr5.2 | CDS | 14502499 | 14502569 | 14502499 | ID=cds.MS.gene67598.t1;Parent=MS.gene67598.t1 |
| chr5.2 | exon | 14502663 | 14502716 | 14502663 | ID=MS.gene67598.t1.exon4;Parent=MS.gene67598.t1 |
| chr5.2 | CDS | 14502663 | 14502716 | 14502663 | ID=cds.MS.gene67598.t1;Parent=MS.gene67598.t1 |
| chr5.2 | exon | 14503078 | 14503152 | 14503078 | ID=MS.gene67598.t1.exon5;Parent=MS.gene67598.t1 |
| chr5.2 | CDS | 14503078 | 14503152 | 14503078 | ID=cds.MS.gene67598.t1;Parent=MS.gene67598.t1 |
| Gene Sequence |
| Protein sequence |
