Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68036.t1 | XP_003612131.2 | 97.9 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 3.00E-106 | 394.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68036.t1 | G7KGE7 | 97.9 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 2.2e-106 | 394.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene68036 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene68036 | 0.823357 | 1.45E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene68036 | 0.808948 | 2.41E-50 | -1.69E-46 |
| MS.gene053184 | MS.gene68036 | 0.807332 | 5.32E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene68036 | 0.80308 | 4.13E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene68036 | 0.907034 | 7.83E-81 | -1.69E-46 |
| MS.gene055608 | MS.gene68036 | 0.833171 | 6.22E-56 | -1.69E-46 |
| MS.gene055860 | MS.gene68036 | 0.838969 | 2.10E-57 | -1.69E-46 |
| MS.gene058042 | MS.gene68036 | 0.875455 | 3.04E-68 | -1.69E-46 |
| MS.gene058168 | MS.gene68036 | 0.816174 | 6.34E-52 | -1.69E-46 |
| MS.gene059124 | MS.gene68036 | 0.805809 | 1.12E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68036.t1 | MTR_5g021610 | 97.938 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 1.16e-144 | 400 |
| MS.gene68036.t1 | MTR_8g076160 | 75.897 | 195 | 44 | 3 | 1 | 194 | 1 | 193 | 7.99e-108 | 307 |
| MS.gene68036.t1 | MTR_2g005880 | 53.846 | 182 | 80 | 1 | 12 | 193 | 3 | 180 | 1.75e-66 | 202 |
| MS.gene68036.t1 | MTR_4g114080 | 58.571 | 140 | 54 | 1 | 12 | 151 | 5 | 140 | 1.03e-56 | 177 |
| MS.gene68036.t1 | MTR_4g011020 | 55.479 | 146 | 57 | 2 | 12 | 155 | 9 | 148 | 2.02e-55 | 174 |
| MS.gene68036.t1 | MTR_2g103490 | 54.225 | 142 | 60 | 2 | 12 | 152 | 9 | 146 | 3.54e-51 | 162 |
| MS.gene68036.t1 | MTR_2g103460 | 52.817 | 142 | 62 | 2 | 12 | 152 | 9 | 146 | 3.40e-49 | 157 |
| MS.gene68036.t1 | MTR_0003s0630 | 47.333 | 150 | 77 | 2 | 4 | 152 | 26 | 174 | 6.65e-48 | 155 |
| MS.gene68036.t1 | MTR_1g115170 | 46.617 | 133 | 66 | 2 | 29 | 160 | 3 | 131 | 1.64e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68036.t1 | AT1G15380 | 58.993 | 139 | 53 | 1 | 12 | 150 | 9 | 143 | 1.42e-58 | 181 |
| MS.gene68036.t1 | AT1G15380 | 58.993 | 139 | 53 | 1 | 12 | 150 | 9 | 143 | 1.42e-58 | 181 |
| MS.gene68036.t1 | AT1G80160 | 57.639 | 144 | 57 | 2 | 12 | 155 | 9 | 148 | 1.78e-58 | 181 |
| MS.gene68036.t1 | AT1G80160 | 56.944 | 144 | 58 | 2 | 12 | 155 | 50 | 189 | 7.17e-58 | 181 |
| MS.gene68036.t1 | AT2G28420 | 52.083 | 144 | 65 | 2 | 13 | 155 | 17 | 157 | 9.32e-53 | 167 |
| MS.gene68036.t1 | AT1G80160 | 53.000 | 100 | 43 | 2 | 56 | 155 | 27 | 122 | 4.10e-33 | 115 |
Find 33 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATCTTCTTTCAAATTTAC+TGG | 0.206523 | 5.3:-12410189 | MS.gene68036:CDS |
| TGGTTATCCTTGGGATTAAT+AGG | 0.256572 | 5.3:+12409967 | None:intergenic |
| CTCTTAAAGCTATGGCTTCT+AGG | 0.268260 | 5.3:+12409420 | None:intergenic |
| GGTAGAAGATGAAGGAATTA+AGG | 0.296959 | 5.3:-12409547 | MS.gene68036:CDS |
| TAAGAAGAGATTAGAAGAAA+AGG | 0.341886 | 5.3:-12409592 | MS.gene68036:CDS |
| GAAGCAGTGCATGAAGAAAT+AGG | 0.352827 | 5.3:+12409450 | None:intergenic |
| GAATCCTCCACACTTGGAAT+TGG | 0.363613 | 5.3:+12409389 | None:intergenic |
| CATGAAGAAATAGGAATGAT+TGG | 0.374847 | 5.3:+12409459 | None:intergenic |
| AAGAAGAGATTAGAAGAAAA+GGG | 0.385631 | 5.3:-12409591 | MS.gene68036:CDS |
| GAAAGAAGATGGTCGTTTGA+TGG | 0.396618 | 5.3:+12410200 | None:intergenic |
| AGCTCAATCATGTAGCCATC+AGG | 0.416541 | 5.3:+12409501 | None:intergenic |
| GAATGAGATATGGTTATCCT+TGG | 0.430947 | 5.3:+12409957 | None:intergenic |
| GGATAACCATATCTCATTCC+AGG | 0.432811 | 5.3:-12409953 | MS.gene68036:intron |
| GTATCAAATTCATCAATGTC+TGG | 0.469039 | 5.3:+12410009 | None:intergenic |
| AAAGAAGATGGTCGTTTGAT+GGG | 0.476824 | 5.3:+12410201 | None:intergenic |
| CCAGTAAATTTGAAAGAAGA+TGG | 0.495096 | 5.3:+12410189 | None:intergenic |
| ATGAAGAAATAGGAATGATT+GGG | 0.499317 | 5.3:+12409460 | None:intergenic |
| CTGCATGAATCCTCCACACT+TGG | 0.500461 | 5.3:+12409383 | None:intergenic |
| AATGAGATATGGTTATCCTT+GGG | 0.506176 | 5.3:+12409958 | None:intergenic |
| CTTTCAAATTTACTGGAGCA+TGG | 0.508696 | 5.3:-12410182 | MS.gene68036:intron |
| AAGTAGATCTCTTAAAGCTA+TGG | 0.522280 | 5.3:+12409412 | None:intergenic |
| ATTCATGCAGAATGTTATGA+TGG | 0.532345 | 5.3:-12409370 | MS.gene68036:CDS |
| TCATGTAGCCATCAGGGTCA+TGG | 0.545015 | 5.3:+12409508 | None:intergenic |
| AATGAGATATGTTACAGCAT+TGG | 0.548154 | 5.3:-12409568 | MS.gene68036:CDS |
| ACAGCATTGGTAGAAGATGA+AGG | 0.580373 | 5.3:-12409555 | MS.gene68036:CDS |
| GTGTTCTTCCATGACCCTGA+TGG | 0.589307 | 5.3:-12409516 | MS.gene68036:CDS |
| ACATGATTCAATGAGAGCAA+TGG | 0.593307 | 5.3:+12410288 | None:intergenic |
| AACGAGGCCTATTAATCCCA+AGG | 0.600112 | 5.3:-12409974 | MS.gene68036:CDS |
| GCTCAATCATGTAGCCATCA+GGG | 0.602960 | 5.3:+12409502 | None:intergenic |
| TCTACTTCCAATTCCAAGTG+TGG | 0.614729 | 5.3:-12409396 | MS.gene68036:CDS |
| ACTTCCAATTCCAAGTGTGG+AGG | 0.621587 | 5.3:-12409393 | MS.gene68036:CDS |
| TATGATGGAAAGCTTGAGCA+TGG | 0.636263 | 5.3:-12409355 | MS.gene68036:CDS |
| ATACTTACATGAATGAAACG+AGG | 0.744566 | 5.3:-12409990 | MS.gene68036:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGCTTATTTTTTTATATTTT+TGG | - | chr5.3:12409499-12409518 | MS.gene68036:CDS | 10.0% |
| !! | CCAAAAATTTATATACAATA+TGG | - | chr5.3:12410011-12410030 | MS.gene68036:CDS | 15.0% |
| !!! | AATATAGTCATTTTTTGAAA+TGG | - | chr5.3:12409753-12409772 | MS.gene68036:intron | 15.0% |
| !!! | CCATATTGTATATAAATTTT+TGG | + | chr5.3:12410014-12410033 | None:intergenic | 15.0% |
| !!! | TATAAATCTTTATTTTTACC+TGG | + | chr5.3:12409724-12409743 | None:intergenic | 15.0% |
| !! | GCACATAATAATTATAACAA+AGG | + | chr5.3:12409915-12409934 | None:intergenic | 20.0% |
| !! | TGTTATAATTATTATGTGCA+TGG | - | chr5.3:12409916-12409935 | MS.gene68036:intron | 20.0% |
| !!! | AGGTTTTATAATTATGGTAT+TGG | - | chr5.3:12409605-12409624 | MS.gene68036:CDS | 20.0% |
| !!! | TTTTGCAGGTTTTATAATTA+TGG | - | chr5.3:12409599-12409618 | MS.gene68036:CDS | 20.0% |
| ! | AAGAAGAGATTAGAAGAAAA+GGG | - | chr5.3:12410065-12410084 | MS.gene68036:intron | 25.0% |
| ! | ATGAAGAAATAGGAATGATT+GGG | + | chr5.3:12410199-12410218 | None:intergenic | 25.0% |
| ! | TAAGAAGAGATTAGAAGAAA+AGG | - | chr5.3:12410064-12410083 | MS.gene68036:intron | 25.0% |
| ! | TAAGTAAATAATTAACCGCA+TGG | - | chr5.3:12409948-12409967 | MS.gene68036:intron | 25.0% |
| !! | CAATTTTATGAGGATGTTTT+GGG | - | chr5.3:12409425-12409444 | MS.gene68036:CDS | 25.0% |
| !!! | GCTTTTAACTTTTTTTTTGC+AGG | - | chr5.3:12409585-12409604 | MS.gene68036:CDS | 25.0% |
| !!! | GTCATTTTTTGAAATGGTTA+TGG | - | chr5.3:12409759-12409778 | MS.gene68036:intron | 25.0% |
| AAGTAGATCTCTTAAAGCTA+TGG | + | chr5.3:12410247-12410266 | None:intergenic | 30.0% | |
| AATGAGATATGGTTATCCTT+GGG | + | chr5.3:12409701-12409720 | None:intergenic | 30.0% | |
| AATGAGATATGTTACAGCAT+TGG | - | chr5.3:12410088-12410107 | MS.gene68036:intron | 30.0% | |
| ATACTTACATGAATGAAACG+AGG | - | chr5.3:12409666-12409685 | MS.gene68036:intron | 30.0% | |
| ATGGAAATCTATGCATATGT+AGG | - | chr5.3:12409813-12409832 | MS.gene68036:intron | 30.0% | |
| ATTCATGCAGAATGTTATGA+TGG | - | chr5.3:12410286-12410305 | MS.gene68036:CDS | 30.0% | |
| CATGAAGAAATAGGAATGAT+TGG | + | chr5.3:12410200-12410219 | None:intergenic | 30.0% | |
| CCAGTAAATTTGAAAGAAGA+TGG | + | chr5.3:12409470-12409489 | None:intergenic | 30.0% | |
| CCATCTTCTTTCAAATTTAC+TGG | - | chr5.3:12409467-12409486 | MS.gene68036:CDS | 30.0% | |
| GTATCAAATTCATCAATGTC+TGG | + | chr5.3:12409650-12409669 | None:intergenic | 30.0% | |
| TGGAAATCTATGCATATGTA+GGG | - | chr5.3:12409814-12409833 | MS.gene68036:intron | 30.0% | |
| ! | GCAATTTTATGAGGATGTTT+TGG | - | chr5.3:12409424-12409443 | MS.gene68036:CDS | 30.0% |
| ! | GGAATCTATGCAATTTTATG+AGG | - | chr5.3:12409415-12409434 | MS.gene68036:CDS | 30.0% |
| ! | TTTTTACCTGGAATGAGATA+TGG | + | chr5.3:12409712-12409731 | None:intergenic | 30.0% |
| !!! | ATATGGTTTTTTGCAGTGTA+CGG | - | chr5.3:12410028-12410047 | MS.gene68036:CDS | 30.0% |
| AAAGAAGATGGTCGTTTGAT+GGG | + | chr5.3:12409458-12409477 | None:intergenic | 35.0% | |
| ACATGATTCAATGAGAGCAA+TGG | + | chr5.3:12409371-12409390 | None:intergenic | 35.0% | |
| AGCTAAATTTGTGATCCATG+CGG | + | chr5.3:12409966-12409985 | None:intergenic | 35.0% | |
| CTTTCAAATTTACTGGAGCA+TGG | - | chr5.3:12409474-12409493 | MS.gene68036:CDS | 35.0% | |
| GAATGAGATATGGTTATCCT+TGG | + | chr5.3:12409702-12409721 | None:intergenic | 35.0% | |
| GGTAGAAGATGAAGGAATTA+AGG | - | chr5.3:12410109-12410128 | MS.gene68036:intron | 35.0% | |
| TGGTTATCCTTGGGATTAAT+AGG | + | chr5.3:12409692-12409711 | None:intergenic | 35.0% | |
| ! | TCTTTTGTGTAGATCAGTGT+TGG | - | chr5.3:12409394-12409413 | MS.gene68036:CDS | 35.0% |
| CTCTTAAAGCTATGGCTTCT+AGG | + | chr5.3:12410239-12410258 | None:intergenic | 40.0% | |
| GAAAGAAGATGGTCGTTTGA+TGG | + | chr5.3:12409459-12409478 | None:intergenic | 40.0% | |
| GAAGCAGTGCATGAAGAAAT+AGG | + | chr5.3:12410209-12410228 | None:intergenic | 40.0% | |
| GGATAACCATATCTCATTCC+AGG | - | chr5.3:12409703-12409722 | MS.gene68036:intron | 40.0% | |
| TCTACTTCCAATTCCAAGTG+TGG | - | chr5.3:12410260-12410279 | MS.gene68036:CDS | 40.0% | |
| ! | TATGATGGAAAGCTTGAGCA+TGG | - | chr5.3:12410301-12410320 | MS.gene68036:CDS | 40.0% |
| !! | ACAGCATTGGTAGAAGATGA+AGG | - | chr5.3:12410101-12410120 | MS.gene68036:intron | 40.0% |
| AACGAGGCCTATTAATCCCA+AGG | - | chr5.3:12409682-12409701 | MS.gene68036:intron | 45.0% | |
| ACTTCCAATTCCAAGTGTGG+AGG | - | chr5.3:12410263-12410282 | MS.gene68036:CDS | 45.0% | |
| AGCTCAATCATGTAGCCATC+AGG | + | chr5.3:12410158-12410177 | None:intergenic | 45.0% | |
| GAATCCTCCACACTTGGAAT+TGG | + | chr5.3:12410270-12410289 | None:intergenic | 45.0% | |
| GCTCAATCATGTAGCCATCA+GGG | + | chr5.3:12410157-12410176 | None:intergenic | 45.0% | |
| !! | ATAAATTTATATACTAAAAA+TGG | - | chr5.3:12409794-12409813 | MS.gene68036:intron | 5.0% |
| CTGCATGAATCCTCCACACT+TGG | + | chr5.3:12410276-12410295 | None:intergenic | 50.0% | |
| GTGTTCTTCCATGACCCTGA+TGG | - | chr5.3:12410140-12410159 | MS.gene68036:intron | 50.0% | |
| TCATGTAGCCATCAGGGTCA+TGG | + | chr5.3:12410151-12410170 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 12409332 | 12410346 | 12409332 | ID=MS.gene68036 |
| chr5.3 | mRNA | 12409332 | 12410346 | 12409332 | ID=MS.gene68036.t1;Parent=MS.gene68036 |
| chr5.3 | exon | 12410183 | 12410346 | 12410183 | ID=MS.gene68036.t1.exon1;Parent=MS.gene68036.t1 |
| chr5.3 | CDS | 12410183 | 12410346 | 12410183 | ID=cds.MS.gene68036.t1;Parent=MS.gene68036.t1 |
| chr5.3 | exon | 12409954 | 12410071 | 12409954 | ID=MS.gene68036.t1.exon2;Parent=MS.gene68036.t1 |
| chr5.3 | CDS | 12409954 | 12410071 | 12409954 | ID=cds.MS.gene68036.t1;Parent=MS.gene68036.t1 |
| chr5.3 | exon | 12409332 | 12409634 | 12409332 | ID=MS.gene68036.t1.exon3;Parent=MS.gene68036.t1 |
| chr5.3 | CDS | 12409332 | 12409634 | 12409332 | ID=cds.MS.gene68036.t1;Parent=MS.gene68036.t1 |
| Gene Sequence |
| Protein sequence |
