Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68265.t1 | GAU28582.1 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 9.00E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68265.t1 | Q38859 | 86.8 | 106 | 14 | 0 | 1 | 106 | 1 | 106 | 8.0e-49 | 194.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68265.t1 | A0A2Z6MUC9 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 6.5e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68265 | MS.gene44023 | PPI |
| MS.gene68265 | MS.gene90747 | PPI |
| MS.gene68265 | MS.gene70805 | PPI |
| MS.gene00182 | MS.gene68265 | PPI |
| MS.gene68265 | MS.gene69921 | PPI |
| MS.gene68265 | MS.gene24205 | PPI |
| MS.gene68265 | MS.gene56011 | PPI |
| MS.gene68265 | MS.gene005152 | PPI |
| MS.gene68265 | MS.gene063673 | PPI |
| MS.gene68265 | MS.gene27287 | PPI |
| MS.gene058216 | MS.gene68265 | PPI |
| MS.gene68265 | MS.gene65289 | PPI |
| MS.gene68265 | MS.gene67503 | PPI |
| MS.gene68265 | MS.gene27025 | PPI |
| MS.gene68265 | MS.gene64820 | PPI |
| MS.gene68265 | MS.gene67685 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68265.t1 | MTR_4g037750 | 94.545 | 110 | 6 | 0 | 1 | 110 | 1 | 110 | 1.26e-74 | 218 |
| MS.gene68265.t1 | MTR_6g004540 | 93.333 | 75 | 5 | 0 | 1 | 75 | 1 | 75 | 7.56e-49 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68265.t1 | AT3G52090 | 86.792 | 106 | 14 | 0 | 1 | 106 | 1 | 106 | 9.35e-68 | 201 |
| MS.gene68265.t1 | AT3G52090 | 67.647 | 136 | 14 | 1 | 1 | 106 | 1 | 136 | 1.98e-60 | 183 |
Find 32 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACATAAAGTTGATAGTT+TGG | 0.275803 | 4.1:-60074729 | MS.gene68265:CDS |
| AAGCGTTCTTTGCATGCATT+TGG | 0.326468 | 4.1:-60074779 | MS.gene68265:CDS |
| TATCCAGCGAATAACACATT+AGG | 0.348978 | 4.1:+60076052 | None:intergenic |
| GTAACGATCGGGTGCATTCA+TGG | 0.369473 | 4.1:+60076407 | None:intergenic |
| TTCAGTACAAAATTATCATC+AGG | 0.382359 | 4.1:-60076012 | MS.gene68265:CDS |
| TAGTTTGGATTGGATGTGTT+TGG | 0.388311 | 4.1:-60074714 | MS.gene68265:CDS |
| TACCTCAAAACCACTCTTCA+AGG | 0.393375 | 4.1:+60075102 | None:intergenic |
| GGGAATAGTTTGTGGAGGAC+AGG | 0.423629 | 4.1:-60074758 | MS.gene68265:CDS |
| TAACCAGTCTATTAATGATC+TGG | 0.435786 | 4.1:-60075140 | MS.gene68265:CDS |
| ATCCAGCGAATAACACATTA+GGG | 0.445494 | 4.1:+60076053 | None:intergenic |
| AGCGTTCTTTGCATGCATTT+GGG | 0.449687 | 4.1:-60074778 | MS.gene68265:CDS |
| AGTGTGATCTTCTCTTTCAA+TGG | 0.460602 | 4.1:+60076208 | None:intergenic |
| ATGTGTTTGGATGATTTAGA+AGG | 0.466519 | 4.1:-60074701 | MS.gene68265:CDS |
| GACCCTAATGTGTTATTCGC+TGG | 0.469437 | 4.1:-60076055 | MS.gene68265:CDS |
| ATAAAGTTGATAGTTTGGAT+TGG | 0.478047 | 4.1:-60074724 | MS.gene68265:CDS |
| CAGCCAGTCTTCACCAATGC+AGG | 0.497359 | 4.1:-60075167 | MS.gene68265:CDS |
| TGTCCAGATCATTAATAGAC+TGG | 0.498287 | 4.1:+60075137 | None:intergenic |
| GACGAAACGCTCGTAACGAT+CGG | 0.522382 | 4.1:+60076395 | None:intergenic |
| ATGCATTTGGGAATAGTTTG+TGG | 0.528527 | 4.1:-60074766 | MS.gene68265:CDS |
| ACGAAACGCTCGTAACGATC+GGG | 0.531962 | 4.1:+60076396 | None:intergenic |
| CTGCATTGGTGAAGACTGGC+TGG | 0.534907 | 4.1:+60075168 | None:intergenic |
| AATAAAGGGTTTCGTACGAG+AGG | 0.538852 | 4.1:-60076262 | MS.gene68265:intron |
| ATGCCTGCATTGGTGAAGAC+TGG | 0.541956 | 4.1:+60075164 | None:intergenic |
| GAGCGTTTCGTCGTCCCCGA+AGG | 0.559317 | 4.1:-60076384 | MS.gene68265:CDS |
| TCAGTACAAAATTATCATCA+GGG | 0.570840 | 4.1:-60076011 | MS.gene68265:intron |
| GAACTTGATACCTTGAAGAG+TGG | 0.588358 | 4.1:-60075112 | MS.gene68265:CDS |
| GAAAGAGAAGATCACACTAT+CGG | 0.589235 | 4.1:-60076203 | MS.gene68265:CDS |
| GACTGGTTATATGCCTGCAT+TGG | 0.605258 | 4.1:+60075154 | None:intergenic |
| CATTTGGGAATAGTTTGTGG+AGG | 0.618905 | 4.1:-60074763 | MS.gene68265:CDS |
| ATAAAGGGTTTCGTACGAGA+GGG | 0.647792 | 4.1:-60076261 | MS.gene68265:intron |
| GTCTATTAATGATCTGGACA+AGG | 0.664690 | 4.1:-60075134 | MS.gene68265:CDS |
| TGGGTAGTACATACATGCGG+AGG | 0.701441 | 4.1:+60076174 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAAAAAATAAAAAAGTT+AGG | + | chr4.1:60074976-60074995 | None:intergenic | 10.0% |
| !! | AACAAAAAATAAAAAAGTTA+GGG | + | chr4.1:60074975-60074994 | None:intergenic | 10.0% |
| !!! | ATTTTTTTTTTAATGTTTTC+AGG | - | chr4.1:60075006-60075025 | MS.gene68265:intron | 10.0% |
| !! | ATATATTTATTTGGAGAAAA+TGG | - | chr4.1:60075403-60075422 | MS.gene68265:intron | 15.0% |
| !!! | ATTTTTTTTCGAGTAAATAA+AGG | - | chr4.1:60074819-60074838 | MS.gene68265:intron | 15.0% |
| !!! | TTTTTTTCTTTTCTTAAAGT+AGG | - | chr4.1:60075817-60075836 | MS.gene68265:intron | 15.0% |
| !!! | TTTTTTTTCGAGTAAATAAA+GGG | - | chr4.1:60074820-60074839 | MS.gene68265:intron | 15.0% |
| !! | AAAAAAAACACAACATACAT+AGG | + | chr4.1:60075800-60075819 | None:intergenic | 20.0% |
| !! | AAAATGAGAAAAAAGAGAAT+AGG | + | chr4.1:60074788-60074807 | None:intergenic | 20.0% |
| !! | AAACAGGCTATATATTTATT+TGG | - | chr4.1:60075394-60075413 | MS.gene68265:intron | 20.0% |
| !! | AAATAAATATATAGCCTGTT+TGG | + | chr4.1:60075395-60075414 | None:intergenic | 20.0% |
| !! | AAGATAAAAAAAGATCCATT+AGG | + | chr4.1:60075500-60075519 | None:intergenic | 20.0% |
| !! | GTATATAAACTGAAAACTTA+TGG | + | chr4.1:60075620-60075639 | None:intergenic | 20.0% |
| !!! | TGTCATTTTTTAATTACTAC+CGG | + | chr4.1:60075158-60075177 | None:intergenic | 20.0% |
| ! | AAAAAGAGAATAGGTAAAGA+AGG | + | chr4.1:60074779-60074798 | None:intergenic | 25.0% |
| ! | ACTTTCTTATAGCAATGAAT+TGG | - | chr4.1:60075528-60075547 | MS.gene68265:intron | 25.0% |
| ! | ATGGAAAATGTAAATAGAAG+CGG | + | chr4.1:60076064-60076083 | None:intergenic | 25.0% |
| ! | ATTTATTTACTCTCCTTTCT+TGG | - | chr4.1:60076084-60076103 | MS.gene68265:intron | 25.0% |
| ! | GAAAGGAGAGTAAATAAATA+TGG | + | chr4.1:60076083-60076102 | None:intergenic | 25.0% |
| ! | TCAGTACAAAATTATCATCA+GGG | - | chr4.1:60075085-60075104 | MS.gene68265:intron | 25.0% |
| ! | TTCAGTACAAAATTATCATC+AGG | - | chr4.1:60075084-60075103 | MS.gene68265:intron | 25.0% |
| !! | ATGATAATTTTGTACTGAAG+AGG | + | chr4.1:60075083-60075102 | None:intergenic | 25.0% |
| !! | ATTTTTATCTGTATGCGAAT+TGG | - | chr4.1:60075858-60075877 | MS.gene68265:intron | 25.0% |
| !! | TAAATGAACTGAATTGGTTT+CGG | - | chr4.1:60075473-60075492 | MS.gene68265:intron | 25.0% |
| !! | TTTTTATCTGTATGCGAATT+GGG | - | chr4.1:60075859-60075878 | MS.gene68265:intron | 25.0% |
| !!! | AGAACATAAAGTTGATAGTT+TGG | - | chr4.1:60076367-60076386 | MS.gene68265:intron | 25.0% |
| !!! | ATAAAGTTGATAGTTTGGAT+TGG | - | chr4.1:60076372-60076391 | MS.gene68265:intron | 25.0% |
| AAAAACGAAGAAAGAGAAAG+TGG | + | chr4.1:60074945-60074964 | None:intergenic | 30.0% | |
| AAAACGAAGAAAGAGAAAGT+GGG | + | chr4.1:60074944-60074963 | None:intergenic | 30.0% | |
| AAATAAGCTGAAAACAGCTT+AGG | - | chr4.1:60075286-60075305 | MS.gene68265:intron | 30.0% | |
| ATGTGTTTGGATGATTTAGA+AGG | - | chr4.1:60076395-60076414 | MS.gene68265:CDS | 30.0% | |
| CATGTGTAAATGAACTGAAT+TGG | - | chr4.1:60075467-60075486 | MS.gene68265:intron | 30.0% | |
| TAACCAGTCTATTAATGATC+TGG | - | chr4.1:60075956-60075975 | MS.gene68265:intron | 30.0% | |
| TCAAATAAGTCAATCCAAAC+AGG | - | chr4.1:60075378-60075397 | MS.gene68265:intron | 30.0% | |
| TGATTTATCTGTCTTATCCA+AGG | + | chr4.1:60075449-60075468 | None:intergenic | 30.0% | |
| TGTATAAGAATTGCCAAGAA+AGG | + | chr4.1:60076100-60076119 | None:intergenic | 30.0% | |
| TTCATTGCTATAAGAAAGTG+TGG | + | chr4.1:60075527-60075546 | None:intergenic | 30.0% | |
| ! | GACATTTTCATAAGCTATCT+TGG | - | chr4.1:60075251-60075270 | MS.gene68265:intron | 30.0% |
| !!! | AGATACTTTTTTATGCCTTC+GGG | + | chr4.1:60074730-60074749 | None:intergenic | 30.0% |
| !!! | GAGATACTTTTTTATGCCTT+CGG | + | chr4.1:60074731-60074750 | None:intergenic | 30.0% |
| AGTGTGATCTTCTCTTTCAA+TGG | + | chr4.1:60074891-60074910 | None:intergenic | 35.0% | |
| ATCCAGCGAATAACACATTA+GGG | + | chr4.1:60075046-60075065 | None:intergenic | 35.0% | |
| ATGCATTTGGGAATAGTTTG+TGG | - | chr4.1:60076330-60076349 | MS.gene68265:intron | 35.0% | |
| CAACATACATAGGTCATGTT+TGG | + | chr4.1:60075790-60075809 | None:intergenic | 35.0% | |
| GAAAGAGAAGATCACACTAT+CGG | - | chr4.1:60074893-60074912 | MS.gene68265:intron | 35.0% | |
| GCATTGAGCTTATTAATCCT+TGG | - | chr4.1:60075429-60075448 | MS.gene68265:intron | 35.0% | |
| GTCTATTAATGATCTGGACA+AGG | - | chr4.1:60075962-60075981 | MS.gene68265:intron | 35.0% | |
| TATCCAGCGAATAACACATT+AGG | + | chr4.1:60075047-60075066 | None:intergenic | 35.0% | |
| TGTAAATAGAAGCGGTATTG+AGG | + | chr4.1:60076056-60076075 | None:intergenic | 35.0% | |
| TGTCCAGATCATTAATAGAC+TGG | + | chr4.1:60075962-60075981 | None:intergenic | 35.0% | |
| ! | ATTTTGTACTGAAGAGGATG+AGG | + | chr4.1:60075077-60075096 | None:intergenic | 35.0% |
| ! | CAAATTTTTCCATGACCACT+GGG | + | chr4.1:60076253-60076272 | None:intergenic | 35.0% |
| ! | TAGTTTGGATTGGATGTGTT+TGG | - | chr4.1:60076382-60076401 | MS.gene68265:CDS | 35.0% |
| !!! | GATACTTTTTTATGCCTTCG+GGG | + | chr4.1:60074729-60074748 | None:intergenic | 35.0% |
| AAGCGTTCTTTGCATGCATT+TGG | - | chr4.1:60076317-60076336 | MS.gene68265:intron | 40.0% | |
| AAGTGGGTAGTACATACATG+CGG | + | chr4.1:60074928-60074947 | None:intergenic | 40.0% | |
| AATAAAGGGTTTCGTACGAG+AGG | - | chr4.1:60074834-60074853 | MS.gene68265:intron | 40.0% | |
| AGCGTTCTTTGCATGCATTT+GGG | - | chr4.1:60076318-60076337 | MS.gene68265:intron | 40.0% | |
| ATAAAGGGTTTCGTACGAGA+GGG | - | chr4.1:60074835-60074854 | MS.gene68265:intron | 40.0% | |
| CCAGTGGTCATGGAAAAATT+TGG | - | chr4.1:60076251-60076270 | MS.gene68265:CDS | 40.0% | |
| GAACTTGATACCTTGAAGAG+TGG | - | chr4.1:60075984-60076003 | MS.gene68265:intron | 40.0% | |
| GAGTTATATACGCTGAACCT+CGG | + | chr4.1:60076283-60076302 | None:intergenic | 40.0% | |
| GTTGTATGCATAAGCTACTC+TGG | + | chr4.1:60075648-60075667 | None:intergenic | 40.0% | |
| TACCTCAAAACCACTCTTCA+AGG | + | chr4.1:60075997-60076016 | None:intergenic | 40.0% | |
| TCAGTGTCTGTGCTTCAAAT+GGG | + | chr4.1:60075125-60075144 | None:intergenic | 40.0% | |
| ! | AGATTTGTCATTTGACCCAG+TGG | - | chr4.1:60076235-60076254 | MS.gene68265:CDS | 40.0% |
| ! | CATTTGGGAATAGTTTGTGG+AGG | - | chr4.1:60076333-60076352 | MS.gene68265:intron | 40.0% |
| ! | CCAAATTTTTCCATGACCAC+TGG | + | chr4.1:60076254-60076273 | None:intergenic | 40.0% |
| ! | GAAAAATTTGGAGTTGACCG+AGG | - | chr4.1:60076263-60076282 | MS.gene68265:intron | 40.0% |
| ! | TGAATTGGTTTCGGTCCTAA+TGG | - | chr4.1:60075482-60075501 | MS.gene68265:intron | 40.0% |
| !! | TACCTTGAAGAGTGGTTTTG+AGG | - | chr4.1:60075992-60076011 | MS.gene68265:intron | 40.0% |
| CTTCCAAACAGTGTCACAAG+CGG | - | chr4.1:60075337-60075356 | MS.gene68265:intron | 45.0% | |
| GCTGAAAACAGCTTAGGAAG+AGG | - | chr4.1:60075292-60075311 | MS.gene68265:intron | 45.0% | |
| GTATTAGCGCTATTACTGCC+AGG | - | chr4.1:60076131-60076150 | MS.gene68265:intron | 45.0% | |
| GTCAGTGTCTGTGCTTCAAA+TGG | + | chr4.1:60075126-60075145 | None:intergenic | 45.0% | |
| TAACCGCTTGTGACACTGTT+TGG | + | chr4.1:60075343-60075362 | None:intergenic | 45.0% | |
| ! | GACCCTAATGTGTTATTCGC+TGG | - | chr4.1:60075041-60075060 | MS.gene68265:intron | 45.0% |
| ! | GACTGGTTATATGCCTGCAT+TGG | + | chr4.1:60075945-60075964 | None:intergenic | 45.0% |
| !!! | TTATTTTTTGTTTTTTAATT+TGG | - | chr4.1:60074983-60075002 | MS.gene68265:intron | 5.0% |
| ACGAAACGCTCGTAACGATC+GGG | + | chr4.1:60074703-60074722 | None:intergenic | 50.0% | |
| GACGAAACGCTCGTAACGAT+CGG | + | chr4.1:60074704-60074723 | None:intergenic | 50.0% | |
| GTCATTTGACCCAGTGGTCA+TGG | - | chr4.1:60076241-60076260 | MS.gene68265:CDS | 50.0% | |
| TGGGTAGTACATACATGCGG+AGG | + | chr4.1:60074925-60074944 | None:intergenic | 50.0% | |
| ! | ATGCCTGCATTGGTGAAGAC+TGG | + | chr4.1:60075935-60075954 | None:intergenic | 50.0% |
| ! | GGGAATAGTTTGTGGAGGAC+AGG | - | chr4.1:60076338-60076357 | MS.gene68265:intron | 50.0% |
| AGACACTGACACGTCGACAC+CGG | - | chr4.1:60075136-60075155 | MS.gene68265:CDS | 55.0% | |
| AGCGCTATTACTGCCAGGCT+AGG | - | chr4.1:60076136-60076155 | MS.gene68265:intron | 55.0% | |
| CAGCCAGTCTTCACCAATGC+AGG | - | chr4.1:60075929-60075948 | MS.gene68265:intron | 55.0% | |
| ! | ACTGGCTGGTCGTGTGTATC+TGG | + | chr4.1:60075917-60075936 | None:intergenic | 55.0% |
| ! | CTGCATTGGTGAAGACTGGC+TGG | + | chr4.1:60075931-60075950 | None:intergenic | 55.0% |
| AACGACTAGCGAGCCTAGCC+TGG | + | chr4.1:60076152-60076171 | None:intergenic | 60.0% | |
| GAGCGTTTCGTCGTCCCCGA+AGG | - | chr4.1:60074712-60074731 | MS.gene68265:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 60074691 | 60076427 | 60074691 | ID=MS.gene68265 |
| chr4.1 | mRNA | 60074691 | 60076427 | 60074691 | ID=MS.gene68265.t1;Parent=MS.gene68265 |
| chr4.1 | exon | 60076375 | 60076427 | 60076375 | ID=MS.gene68265.t1.exon1;Parent=MS.gene68265.t1 |
| chr4.1 | CDS | 60076375 | 60076427 | 60076375 | ID=cds.MS.gene68265.t1;Parent=MS.gene68265.t1 |
| chr4.1 | exon | 60076188 | 60076277 | 60076188 | ID=MS.gene68265.t1.exon2;Parent=MS.gene68265.t1 |
| chr4.1 | CDS | 60076188 | 60076277 | 60076188 | ID=cds.MS.gene68265.t1;Parent=MS.gene68265.t1 |
| chr4.1 | exon | 60076012 | 60076090 | 60076012 | ID=MS.gene68265.t1.exon3;Parent=MS.gene68265.t1 |
| chr4.1 | CDS | 60076012 | 60076090 | 60076012 | ID=cds.MS.gene68265.t1;Parent=MS.gene68265.t1 |
| chr4.1 | exon | 60075105 | 60075200 | 60075105 | ID=MS.gene68265.t1.exon4;Parent=MS.gene68265.t1 |
| chr4.1 | CDS | 60075105 | 60075200 | 60075105 | ID=cds.MS.gene68265.t1;Parent=MS.gene68265.t1 |
| chr4.1 | exon | 60074691 | 60074801 | 60074691 | ID=MS.gene68265.t1.exon5;Parent=MS.gene68265.t1 |
| chr4.1 | CDS | 60074691 | 60074801 | 60074691 | ID=cds.MS.gene68265.t1;Parent=MS.gene68265.t1 |
| Gene Sequence |
| Protein sequence |
