Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005152.t1 | XP_003591024.1 | 100 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.90E-51 | 211.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005152.t1 | Q9SS17 | 89.5 | 105 | 11 | 0 | 1 | 105 | 1 | 105 | 5.3e-50 | 198.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005152.t1 | B7FMK4 | 100.0 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.4e-51 | 211.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene055644 | MS.gene005152 | PPI |
| MS.gene005152 | MS.gene70805 | PPI |
| MS.gene005152 | MS.gene69921 | PPI |
| MS.gene005152 | MS.gene83444 | PPI |
| MS.gene41083 | MS.gene005152 | PPI |
| MS.gene005152 | MS.gene54903 | PPI |
| MS.gene005152 | MS.gene055646 | PPI |
| MS.gene028153 | MS.gene005152 | PPI |
| MS.gene41081 | MS.gene005152 | PPI |
| MS.gene68265 | MS.gene005152 | PPI |
| MS.gene005152 | MS.gene72768 | PPI |
| MS.gene69147 | MS.gene005152 | PPI |
| MS.gene005152 | MS.gene44023 | PPI |
| MS.gene005152 | MS.gene025602 | PPI |
| MS.gene034801 | MS.gene005152 | PPI |
| MS.gene005152 | MS.gene82426 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005152.t1 | MTR_1g081410 | 100.000 | 137 | 0 | 0 | 1 | 137 | 1 | 137 | 2.53e-96 | 273 |
| MS.gene005152.t1 | MTR_7g114640 | 85.401 | 137 | 19 | 1 | 1 | 137 | 1 | 136 | 6.19e-80 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005152.t1 | AT5G28060 | 87.121 | 132 | 17 | 0 | 1 | 132 | 1 | 132 | 3.98e-84 | 242 |
| MS.gene005152.t1 | AT3G04920 | 90.152 | 132 | 13 | 0 | 1 | 132 | 1 | 132 | 8.64e-77 | 224 |
| MS.gene005152.t1 | AT3G04920 | 85.437 | 103 | 14 | 1 | 1 | 102 | 1 | 103 | 7.31e-53 | 162 |
Find 36 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGTTGGTCATGAACTTTC+TGG | 0.100542 | 1.1:-60421810 | None:intergenic |
| TTCAAGTTCAGAACTCATTT+TGG | 0.214352 | 1.1:+60422347 | MS.gene005152:CDS |
| TTGGAAACATTTGCCCTTCC+AGG | 0.258852 | 1.1:-60422121 | None:intergenic |
| GGCGGCAAGTCCACTGGTTT+TGG | 0.272758 | 1.1:+60422371 | MS.gene005152:CDS |
| ACCCTAACAAGTCTGTATTT+TGG | 0.279167 | 1.1:-60422434 | None:intergenic |
| GAAACTCACGAATTGCTTCC+TGG | 0.351970 | 1.1:-60421840 | None:intergenic |
| AACCAAAATACAGACTTGTT+AGG | 0.354436 | 1.1:+60422432 | MS.gene005152:CDS |
| AAATGAAGGAAAGAAAGAAC+AGG | 0.391792 | 1.1:+60423399 | MS.gene005152:CDS |
| CTTGGCAGCATCACCAGCCT+TGG | 0.412083 | 1.1:-60423543 | None:intergenic |
| GCTGGTGATGCTGCCAAGGC+TGG | 0.425056 | 1.1:+60423548 | MS.gene005152:CDS |
| GTCATTGATGTTCTTCATCC+TGG | 0.431746 | 1.1:+60422103 | MS.gene005152:CDS |
| AACAACAACAATGGCAGACA+AGG | 0.443820 | 1.1:+60421772 | None:intergenic |
| TTATTTCTTCTTGCCAGCCT+TGG | 0.476966 | 1.1:-60423561 | None:intergenic |
| TGCAAGCTTCTCCTTTAGCT+CGG | 0.485383 | 1.1:-60422285 | None:intergenic |
| TGACCAACAGGCTTCTCTCC+AGG | 0.487847 | 1.1:+60421822 | MS.gene005152:CDS |
| CCAGAAAGTTCATGACCAAC+AGG | 0.493715 | 1.1:+60421810 | MS.gene005152:CDS |
| GTAGAATGGACTTGATACTA+AGG | 0.495750 | 1.1:+60423358 | MS.gene005152:intron |
| CAAGGCTGGTGATGCTGCCA+AGG | 0.500785 | 1.1:+60423544 | MS.gene005152:CDS |
| TGGTGTGAAGGCCGAGCTAA+AGG | 0.502934 | 1.1:+60422274 | MS.gene005152:intron |
| TTTGGAGGCGGCAAGTCCAC+TGG | 0.503274 | 1.1:+60422365 | MS.gene005152:CDS |
| ACCAAAATACAGACTTGTTA+GGG | 0.511637 | 1.1:+60422433 | MS.gene005152:CDS |
| GAAGATCCGTGGAGTTAAGA+AGG | 0.518538 | 1.1:+60423427 | MS.gene005152:CDS |
| CATGAACTTTCTGGTTCTGA+TGG | 0.533482 | 1.1:-60421801 | None:intergenic |
| TTGATGTTCTTCATCCTGGA+AGG | 0.545793 | 1.1:+60422107 | MS.gene005152:CDS |
| ATACTAAGGTTGAAAAGTCA+AGG | 0.557383 | 1.1:+60423372 | MS.gene005152:CDS |
| GCTTTACCTTCTTAACTCCA+CGG | 0.569745 | 1.1:-60423433 | None:intergenic |
| CTTTCTGGTTCTGATGGTAA+CGG | 0.571013 | 1.1:-60421795 | None:intergenic |
| TGCAAGGATTTACGATGTGA+AGG | 0.571848 | 1.1:+60422304 | MS.gene005152:CDS |
| AAAGTCAAGGAAGCAAATGA+AGG | 0.574321 | 1.1:+60423385 | MS.gene005152:CDS |
| AGCTAAAGGAGAAGCTTGCA+AGG | 0.575032 | 1.1:+60422288 | MS.gene005152:CDS |
| CTTCCTGGAGAGAAGCCTGT+TGG | 0.575264 | 1.1:-60421825 | None:intergenic |
| TGATGTTCTTCATCCTGGAA+GGG | 0.593002 | 1.1:+60422108 | MS.gene005152:CDS |
| TGGAAGGGCAAATGTTTCCA+AGG | 0.598113 | 1.1:+60422123 | MS.gene005152:CDS |
| AACAGGGCAAAGAAGATCCG+TGG | 0.612698 | 1.1:+60423416 | MS.gene005152:CDS |
| ATAGATCAAACCAAAACCAG+TGG | 0.662445 | 1.1:-60422381 | None:intergenic |
| AATGAAGGAAAGAAAGAACA+GGG | 0.674567 | 1.1:+60423400 | MS.gene005152:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGATTGAAATTAAATGT+AGG | - | chr1.1:60422957-60422976 | None:intergenic | 15.0% |
| !! | AATGATTGAAATTAAATGTA+GGG | - | chr1.1:60422956-60422975 | None:intergenic | 15.0% |
| !! | CTTGAAAAAAATAAATGAAT+AGG | - | chr1.1:60422801-60422820 | None:intergenic | 15.0% |
| !!! | ATTTATGTCGAATTATTTTT+AGG | + | chr1.1:60422044-60422063 | MS.gene005152:intron | 15.0% |
| !! | AAAAAACAAACAAATCCATT+TGG | - | chr1.1:60422640-60422659 | None:intergenic | 20.0% |
| !! | ACAAATAAACCACAAAAAAA+AGG | - | chr1.1:60422506-60422525 | None:intergenic | 20.0% |
| !! | ATACAGATCTAATAAAGAAA+GGG | - | chr1.1:60421871-60421890 | None:intergenic | 20.0% |
| !! | CATCACACAATAAAAAAAAT+CGG | - | chr1.1:60422080-60422099 | None:intergenic | 20.0% |
| !! | TAGTATGATTAGAATTAGTA+TGG | + | chr1.1:60422018-60422037 | MS.gene005152:intron | 20.0% |
| !! | TATACAGATCTAATAAAGAA+AGG | - | chr1.1:60421872-60421891 | None:intergenic | 20.0% |
| !!! | AATGGATTTGTTTGTTTTTT+CGG | + | chr1.1:60422640-60422659 | MS.gene005152:intron | 20.0% |
| !!! | TTTTCTTTTCTATTGTAGAA+TGG | + | chr1.1:60423344-60423363 | MS.gene005152:intron | 20.0% |
| !!! | TTTTTTTTATTGTGTGATGA+AGG | + | chr1.1:60422081-60422100 | MS.gene005152:intron | 20.0% |
| ! | ACTAAATTCACAAGTAAATG+AGG | - | chr1.1:60422767-60422786 | None:intergenic | 25.0% |
| ! | ATAAGCTATGAATGTTAGAT+AGG | + | chr1.1:60422165-60422184 | MS.gene005152:intron | 25.0% |
| ! | CTGAAACTTACGTTATATTA+TGG | + | chr1.1:60422215-60422234 | MS.gene005152:intron | 25.0% |
| ! | GATACAAATAACAAATACCT+TGG | - | chr1.1:60422143-60422162 | None:intergenic | 25.0% |
| ! | TACGTTATATTATGGTGTAT+TGG | + | chr1.1:60422223-60422242 | MS.gene005152:intron | 25.0% |
| ! | TAGTTTAGAGATAATTTAGC+TGG | + | chr1.1:60423025-60423044 | MS.gene005152:intron | 25.0% |
| !! | CAAGAGAGTACATAATTATA+GGG | - | chr1.1:60423061-60423080 | None:intergenic | 25.0% |
| !! | TATTTCTGTTAAATAGTTCC+TGG | + | chr1.1:60422887-60422906 | MS.gene005152:intron | 25.0% |
| !! | TCAAGAGAGTACATAATTAT+AGG | - | chr1.1:60423062-60423081 | None:intergenic | 25.0% |
| !!! | TTGTTTGTTTTTTCGGAATT+TGG | + | chr1.1:60422647-60422666 | MS.gene005152:intron | 25.0% |
| AAATGAAGGAAAGAAAGAAC+AGG | + | chr1.1:60423399-60423418 | MS.gene005152:CDS | 30.0% | |
| AATGAAGGAAAGAAAGAACA+GGG | + | chr1.1:60423400-60423419 | MS.gene005152:CDS | 30.0% | |
| ATACTAAGGTTGAAAAGTCA+AGG | + | chr1.1:60423372-60423391 | MS.gene005152:CDS | 30.0% | |
| GATGATTTATATTGTCTCAG+AGG | + | chr1.1:60422192-60422211 | MS.gene005152:intron | 30.0% | |
| GATTCATCAACTACATTAAC+AGG | - | chr1.1:60423265-60423284 | None:intergenic | 30.0% | |
| GATTGAAATTAAATGTAGGG+TGG | - | chr1.1:60422953-60422972 | None:intergenic | 30.0% | |
| TGAACACAAAAACAGTGTTT+GGG | - | chr1.1:60422331-60422350 | None:intergenic | 30.0% | |
| TTGAACACAAAAACAGTGTT+TGG | - | chr1.1:60422332-60422351 | None:intergenic | 30.0% | |
| ! | AACCAAAATACAGACTTGTT+AGG | + | chr1.1:60422432-60422451 | MS.gene005152:CDS | 30.0% |
| ! | GTTAATTCTGTCTTTTCTCT+TGG | + | chr1.1:60422459-60422478 | MS.gene005152:intron | 30.0% |
| ! | TGTCTTTTCTCTTGGATAAT+TGG | + | chr1.1:60422467-60422486 | MS.gene005152:intron | 30.0% |
| ! | TTTGCTTGTAGTGTTGTTAT+TGG | + | chr1.1:60423161-60423180 | MS.gene005152:intron | 30.0% |
| !! | ACCAAAATACAGACTTGTTA+GGG | + | chr1.1:60422433-60422452 | MS.gene005152:CDS | 30.0% |
| !! | GATTTATCGTTTGTTGTGTT+TGG | + | chr1.1:60422254-60422273 | MS.gene005152:intron | 30.0% |
| !! | TGATTTAGTTTGCTATTTCC+TGG | + | chr1.1:60422575-60422594 | MS.gene005152:intron | 30.0% |
| !! | TTCAAGTTCAGAACTCATTT+TGG | + | chr1.1:60422347-60422366 | MS.gene005152:CDS | 30.0% |
| !!! | ACTTTTCTGAAATTTGCCAA+GGG | + | chr1.1:60423230-60423249 | MS.gene005152:intron | 30.0% |
| !!! | GCTTTGTAACCTTTTTTTTG+TGG | + | chr1.1:60422494-60422513 | MS.gene005152:intron | 30.0% |
| AAAGTCAAGGAAGCAAATGA+AGG | + | chr1.1:60423385-60423404 | MS.gene005152:CDS | 35.0% | |
| AGTTATTTGTATTGCCTGCT+GGG | + | chr1.1:60423188-60423207 | MS.gene005152:intron | 35.0% | |
| ATAGATCAAACCAAAACCAG+TGG | - | chr1.1:60422384-60422403 | None:intergenic | 35.0% | |
| CAACATCAATGTTAACAACC+AGG | - | chr1.1:60422596-60422615 | None:intergenic | 35.0% | |
| CAACTACATTAACAGGCAAA+AGG | - | chr1.1:60423258-60423277 | None:intergenic | 35.0% | |
| CTGGTTGTTAACATTGATGT+TGG | + | chr1.1:60422594-60422613 | MS.gene005152:intron | 35.0% | |
| TAGTTATTTGTATTGCCTGC+TGG | + | chr1.1:60423187-60423206 | MS.gene005152:intron | 35.0% | |
| TTTCTCTTGGATAATTGGAC+TGG | + | chr1.1:60422472-60422491 | MS.gene005152:intron | 35.0% | |
| ! | AAGTTCAGAACTCATTTTGG+AGG | + | chr1.1:60422350-60422369 | MS.gene005152:CDS | 35.0% |
| ! | ACCCTAACAAGTCTGTATTT+TGG | - | chr1.1:60422437-60422456 | None:intergenic | 35.0% |
| ! | CGTGTTTGTTAACAAGTGAA+GGG | + | chr1.1:60422922-60422941 | MS.gene005152:intron | 35.0% |
| ! | GTAGAATGGACTTGATACTA+AGG | + | chr1.1:60423358-60423377 | MS.gene005152:intron | 35.0% |
| !! | ACATCAGTGTTTTTGCCAAA+TGG | + | chr1.1:60422622-60422641 | MS.gene005152:intron | 35.0% |
| !! | CATTGTATTTTTGCAGACCA+AGG | + | chr1.1:60423526-60423545 | MS.gene005152:intron | 35.0% |
| !!! | CACTTTTCTGAAATTTGCCA+AGG | + | chr1.1:60423229-60423248 | MS.gene005152:intron | 35.0% |
| !!! | TGCACTTCCTTTTGATTTGA+CGG | + | chr1.1:60422847-60422866 | MS.gene005152:intron | 35.0% |
| ACAAATTCAAACGTGAGCAC+TGG | - | chr1.1:60423094-60423113 | None:intergenic | 40.0% | |
| GCTTTACCTTCTTAACTCCA+CGG | - | chr1.1:60423436-60423455 | None:intergenic | 40.0% | |
| GTCATTGATGTTCTTCATCC+TGG | + | chr1.1:60422103-60422122 | MS.gene005152:CDS | 40.0% | |
| TCACGTTTGAATTTGTCCAG+TGG | + | chr1.1:60423098-60423117 | MS.gene005152:intron | 40.0% | |
| TGATGTTCTTCATCCTGGAA+GGG | + | chr1.1:60422108-60422127 | MS.gene005152:CDS | 40.0% | |
| TGCAAGGATTTACGATGTGA+AGG | + | chr1.1:60422304-60422323 | MS.gene005152:CDS | 40.0% | |
| TGGCAAAAACACTGATGTTG+AGG | - | chr1.1:60422620-60422639 | None:intergenic | 40.0% | |
| TTGATGTTCTTCATCCTGGA+AGG | + | chr1.1:60422107-60422126 | MS.gene005152:CDS | 40.0% | |
| ! | GCGTGTTTGTTAACAAGTGA+AGG | + | chr1.1:60422921-60422940 | MS.gene005152:intron | 40.0% |
| ! | TTATTTCTTCTTGCCAGCCT+TGG | - | chr1.1:60423564-60423583 | None:intergenic | 40.0% |
| !! | CATGAACTTTCTGGTTCTGA+TGG | - | chr1.1:60421804-60421823 | None:intergenic | 40.0% |
| !! | CTTTCTGGTTCTGATGGTAA+CGG | - | chr1.1:60421798-60421817 | None:intergenic | 40.0% |
| !! | GTTTGTTGTGTTTGGTGTGA+AGG | + | chr1.1:60422262-60422281 | MS.gene005152:intron | 40.0% |
| !! | TTCAGAACTCATTTTGGAGG+CGG | + | chr1.1:60422353-60422372 | MS.gene005152:CDS | 40.0% |
| !!! | GCACTTCCTTTTGATTTGAC+GGG | + | chr1.1:60422848-60422867 | MS.gene005152:intron | 40.0% |
| AACACGCTTATAACAGCACC+AGG | - | chr1.1:60422908-60422927 | None:intergenic | 45.0% | |
| AGCTAAAGGAGAAGCTTGCA+AGG | + | chr1.1:60422288-60422307 | MS.gene005152:CDS | 45.0% | |
| CCAGAAAGTTCATGACCAAC+AGG | + | chr1.1:60421810-60421829 | MS.gene005152:CDS | 45.0% | |
| CCTGTTGGTCATGAACTTTC+TGG | - | chr1.1:60421813-60421832 | None:intergenic | 45.0% | |
| GAAACTCACGAATTGCTTCC+TGG | - | chr1.1:60421843-60421862 | None:intergenic | 45.0% | |
| GAAGATCCGTGGAGTTAAGA+AGG | + | chr1.1:60423427-60423446 | MS.gene005152:CDS | 45.0% | |
| TACGCTCCCGTCAAATCAAA+AGG | - | chr1.1:60422857-60422876 | None:intergenic | 45.0% | |
| TGCAAGCTTCTCCTTTAGCT+CGG | - | chr1.1:60422288-60422307 | None:intergenic | 45.0% | |
| TGGAAGGGCAAATGTTTCCA+AGG | + | chr1.1:60422123-60422142 | MS.gene005152:CDS | 45.0% | |
| TTGGAAACATTTGCCCTTCC+AGG | - | chr1.1:60422124-60422143 | None:intergenic | 45.0% | |
| ! | TGATTTGACGGGAGCGTATT+TGG | + | chr1.1:60422859-60422878 | MS.gene005152:intron | 45.0% |
| !! | GTATTTTTGCAGACCAAGGC+TGG | + | chr1.1:60423530-60423549 | MS.gene005152:intron | 45.0% |
| AACAGGGCAAAGAAGATCCG+TGG | + | chr1.1:60423416-60423435 | MS.gene005152:CDS | 50.0% | |
| AAGTAGAACATGTGCCCAGC+AGG | - | chr1.1:60423205-60423224 | None:intergenic | 50.0% | |
| ACTGACAGAACTGACACCAC+TGG | - | chr1.1:60423117-60423136 | None:intergenic | 50.0% | |
| TAACAGGCAAAAGGAGCCCT+TGG | - | chr1.1:60423249-60423268 | None:intergenic | 50.0% | |
| CTTCCTGGAGAGAAGCCTGT+TGG | - | chr1.1:60421828-60421847 | None:intergenic | 55.0% | |
| TGACCAACAGGCTTCTCTCC+AGG | + | chr1.1:60421822-60421841 | MS.gene005152:CDS | 55.0% | |
| TGGTGTGAAGGCCGAGCTAA+AGG | + | chr1.1:60422274-60422293 | MS.gene005152:intron | 55.0% | |
| CTTGGCAGCATCACCAGCCT+TGG | - | chr1.1:60423546-60423565 | None:intergenic | 60.0% | |
| TTTGGAGGCGGCAAGTCCAC+TGG | + | chr1.1:60422365-60422384 | MS.gene005152:CDS | 60.0% | |
| ! | CAAGGCTGGTGATGCTGCCA+AGG | + | chr1.1:60423544-60423563 | MS.gene005152:CDS | 60.0% |
| !!! | GGCGGCAAGTCCACTGGTTT+TGG | + | chr1.1:60422371-60422390 | MS.gene005152:CDS | 60.0% |
| ! | GCTGGTGATGCTGCCAAGGC+TGG | + | chr1.1:60423548-60423567 | MS.gene005152:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 60421782 | 60423583 | 60421782 | ID=MS.gene005152 |
| chr1.1 | mRNA | 60421782 | 60423583 | 60421782 | ID=MS.gene005152.t1;Parent=MS.gene005152 |
| chr1.1 | exon | 60421782 | 60421853 | 60421782 | ID=MS.gene005152.t1.exon1;Parent=MS.gene005152.t1 |
| chr1.1 | CDS | 60421782 | 60421853 | 60421782 | ID=cds.MS.gene005152.t1;Parent=MS.gene005152.t1 |
| chr1.1 | exon | 60422103 | 60422144 | 60422103 | ID=MS.gene005152.t1.exon2;Parent=MS.gene005152.t1 |
| chr1.1 | CDS | 60422103 | 60422144 | 60422103 | ID=cds.MS.gene005152.t1;Parent=MS.gene005152.t1 |
| chr1.1 | exon | 60422284 | 60422454 | 60422284 | ID=MS.gene005152.t1.exon3;Parent=MS.gene005152.t1 |
| chr1.1 | CDS | 60422284 | 60422454 | 60422284 | ID=cds.MS.gene005152.t1;Parent=MS.gene005152.t1 |
| chr1.1 | exon | 60423362 | 60423448 | 60423362 | ID=MS.gene005152.t1.exon4;Parent=MS.gene005152.t1 |
| chr1.1 | CDS | 60423362 | 60423448 | 60423362 | ID=cds.MS.gene005152.t1;Parent=MS.gene005152.t1 |
| chr1.1 | exon | 60423542 | 60423583 | 60423542 | ID=MS.gene005152.t1.exon5;Parent=MS.gene005152.t1 |
| chr1.1 | CDS | 60423542 | 60423583 | 60423542 | ID=cds.MS.gene005152.t1;Parent=MS.gene005152.t1 |
| Gene Sequence |
| Protein sequence |
