AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70805


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70805.t1 MTR_1g081410 100.000 137 0 0 1 137 1 137 2.53e-96 273
MS.gene70805.t1 MTR_7g114640 85.401 137 19 1 1 137 1 136 6.19e-80 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70805.t1 AT5G28060 87.121 132 17 0 1 132 1 132 3.98e-84 242
MS.gene70805.t1 AT3G04920 90.152 132 13 0 1 132 1 132 8.64e-77 224
MS.gene70805.t1 AT3G04920 85.437 103 14 1 1 102 1 103 7.31e-53 162

Find 36 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGTTGGTCATGAACTTTC+TGG 0.100542 1.2:+61055212 None:intergenic
TTCAAGTTCAGAACTCATTT+TGG 0.214352 1.2:-61054674 MS.gene70805:CDS
TTGGAAACATTTGCCCTTCC+AGG 0.258852 1.2:+61054900 None:intergenic
GGCGGCAAGTCCACTGGTTT+TGG 0.272758 1.2:-61054650 MS.gene70805:CDS
ACCCTAACAAGTCTGTATTT+TGG 0.279167 1.2:+61054587 None:intergenic
GAAACTCACGAATTGCTTCC+TGG 0.336108 1.2:+61055182 None:intergenic
AACCAAAATACAGACTTGTT+AGG 0.354436 1.2:-61054589 MS.gene70805:CDS
AAATGAAGGAAAGAAAGAAC+AGG 0.391792 1.2:-61053622 MS.gene70805:CDS
CTTGGCAGCATCACCAGCCT+TGG 0.412083 1.2:+61053478 None:intergenic
GCTGGTGATGCTGCCAAGGC+TGG 0.425056 1.2:-61053473 MS.gene70805:CDS
GTCATTGATGTTCTTCATCC+TGG 0.431746 1.2:-61054918 MS.gene70805:CDS
AACAACAACAATGGCAGACA+AGG 0.443820 1.2:-61055250 None:intergenic
TTATTTCTTCTTGCCAGCCT+TGG 0.476966 1.2:+61053460 None:intergenic
TGCAAGCTTCTCCTTTAGCT+CGG 0.485383 1.2:+61054736 None:intergenic
TGACCAACAGGCTTCTCTCC+AGG 0.487847 1.2:-61055200 MS.gene70805:CDS
CCAGAAAGTTCATGACCAAC+AGG 0.493715 1.2:-61055212 MS.gene70805:CDS
GTAGAATGGACTTGATACTA+AGG 0.495750 1.2:-61053663 MS.gene70805:intron
CAAGGCTGGTGATGCTGCCA+AGG 0.500785 1.2:-61053477 MS.gene70805:CDS
TGGTGTGAAGGCCGAGCTAA+AGG 0.502934 1.2:-61054747 MS.gene70805:intron
TTTGGAGGCGGCAAGTCCAC+TGG 0.503274 1.2:-61054656 MS.gene70805:CDS
ACCAAAATACAGACTTGTTA+GGG 0.511637 1.2:-61054588 MS.gene70805:intron
GAAGATCCGTGGAGTTAAGA+AGG 0.518538 1.2:-61053594 MS.gene70805:intron
CATGAACTTTCTGGTTCTGA+TGG 0.533482 1.2:+61055221 None:intergenic
TTGATGTTCTTCATCCTGGA+AGG 0.545793 1.2:-61054914 MS.gene70805:CDS
ATACTAAGGTTGAAAAGTCA+AGG 0.557383 1.2:-61053649 MS.gene70805:CDS
GCTTTACCTTCTTAACTCCA+CGG 0.569745 1.2:+61053588 None:intergenic
CTTTCTGGTTCTGATGGTAA+CGG 0.571013 1.2:+61055227 None:intergenic
TGCAAGGATTTACGATGTGA+AGG 0.571848 1.2:-61054717 MS.gene70805:CDS
AAAGTCAAGGAAGCAAATGA+AGG 0.574321 1.2:-61053636 MS.gene70805:CDS
AGCTAAAGGAGAAGCTTGCA+AGG 0.575032 1.2:-61054733 MS.gene70805:CDS
CTTCCTGGAGAGAAGCCTGT+TGG 0.575264 1.2:+61055197 None:intergenic
TGATGTTCTTCATCCTGGAA+GGG 0.593002 1.2:-61054913 MS.gene70805:CDS
TGGAAGGGCAAATGTTTCCA+AGG 0.598113 1.2:-61054898 MS.gene70805:intron
AACAGGGCAAAGAAGATCCG+TGG 0.612698 1.2:-61053605 MS.gene70805:CDS
ATAGATCAAACCAAAACCAG+TGG 0.662445 1.2:+61054640 None:intergenic
AATGAAGGAAAGAAAGAACA+GGG 0.674567 1.2:-61053621 MS.gene70805:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAACAAATCCATT+TGG + chr1.2:61054319-61054338 None:intergenic 15.0%
!! AAATGATTGAAATTAAATGT+AGG + chr1.2:61054636-61054655 None:intergenic 15.0%
!! AATGATTGAAATTAAATGTA+GGG + chr1.2:61054635-61054654 None:intergenic 15.0%
!! CTTGAAAAAAATAAATGAAT+AGG + chr1.2:61054480-61054499 None:intergenic 15.0%
!!! AATGGATTTGTTTTTTTTTT+CGG - chr1.2:61054319-61054338 MS.gene70805:intron 15.0%
!!! ATTTATGTCGAATTATTTTT+AGG - chr1.2:61053723-61053742 MS.gene70805:intron 15.0%
!! ACAAATAAACCACAAAAAAA+AGG + chr1.2:61054185-61054204 None:intergenic 20.0%
!! ATACAGATCTAATAAAGAAA+GGG + chr1.2:61053550-61053569 None:intergenic 20.0%
!! CATCACACAATAAAAAAAAT+CGG + chr1.2:61053759-61053778 None:intergenic 20.0%
!! TAGTATGATTAGAATTAGTA+TGG - chr1.2:61053697-61053716 MS.gene70805:intron 20.0%
!! TATACAGATCTAATAAAGAA+AGG + chr1.2:61053551-61053570 None:intergenic 20.0%
!!! TTGTTTTTTTTTTCGGAATT+TGG - chr1.2:61054326-61054345 MS.gene70805:intron 20.0%
!!! TTTTCTTTTCTATTGTAGAA+TGG - chr1.2:61055023-61055042 MS.gene70805:intron 20.0%
!!! TTTTTTTTATTGTGTGATGA+AGG - chr1.2:61053760-61053779 MS.gene70805:intron 20.0%
! ATATGCTATGAATGTTAGAT+AGG - chr1.2:61053844-61053863 MS.gene70805:intron 25.0%
! CTGAAACTTACGTTATATTA+TGG - chr1.2:61053894-61053913 MS.gene70805:intron 25.0%
! GATACAAATAACAAATACCT+TGG + chr1.2:61053822-61053841 None:intergenic 25.0%
! TACGTTATATTATGGTGTAT+TGG - chr1.2:61053902-61053921 MS.gene70805:intron 25.0%
! TAGTTTAGAGATAATTTAGC+TGG - chr1.2:61054704-61054723 MS.gene70805:CDS 25.0%
!! CAAGAGAGTACATAATTATA+GGG + chr1.2:61054740-61054759 None:intergenic 25.0%
!! TATTTCTGTTAAATAGTTCC+TGG - chr1.2:61054566-61054585 MS.gene70805:intron 25.0%
!! TCAAGAGAGTACATAATTAT+AGG + chr1.2:61054741-61054760 None:intergenic 25.0%
AAATGAAGGAAAGAAAGAAC+AGG - chr1.2:61055078-61055097 MS.gene70805:intron 30.0%
AATGAAGGAAAGAAAGAACA+GGG - chr1.2:61055079-61055098 MS.gene70805:intron 30.0%
ACTAAATTCACAAGCAAATG+AGG + chr1.2:61054446-61054465 None:intergenic 30.0%
ATACTAAGGTTGAAAAGTCA+AGG - chr1.2:61055051-61055070 MS.gene70805:intron 30.0%
GATGATTTATATTGTCTCAG+AGG - chr1.2:61053871-61053890 MS.gene70805:intron 30.0%
GATTCATCAACTACATTAAC+AGG + chr1.2:61054944-61054963 None:intergenic 30.0%
GATTGAAATTAAATGTAGGG+TGG + chr1.2:61054632-61054651 None:intergenic 30.0%
TCTAATAAAGAAAGGGAAGA+AGG + chr1.2:61053543-61053562 None:intergenic 30.0%
TGAACACAAAAACAGTGTTT+GGG + chr1.2:61054010-61054029 None:intergenic 30.0%
TTGAACACAAAAACAGTGTT+TGG + chr1.2:61054011-61054030 None:intergenic 30.0%
! AACCAAAATACAGACTTGTT+AGG - chr1.2:61054111-61054130 MS.gene70805:intron 30.0%
! GTTAATTCTGTCTTTTCTCT+TGG - chr1.2:61054138-61054157 MS.gene70805:intron 30.0%
! TGTCTTTTCTCTTGGATAAT+TGG - chr1.2:61054146-61054165 MS.gene70805:intron 30.0%
! TGTGTTTGTTAACAAGTGAA+GGG - chr1.2:61054601-61054620 MS.gene70805:CDS 30.0%
! TTTGCTTGTAGTGTTGTTAT+TGG - chr1.2:61054840-61054859 MS.gene70805:intron 30.0%
!! ACCAAAATACAGACTTGTTA+GGG - chr1.2:61054112-61054131 MS.gene70805:intron 30.0%
!! GATTTATCGTTTGTTGTGTT+TGG - chr1.2:61053933-61053952 MS.gene70805:intron 30.0%
!! TGATTTAGTTTGCTATTTCC+TGG - chr1.2:61054254-61054273 MS.gene70805:intron 30.0%
!! TTCAAGTTCAGAACTCATTT+TGG - chr1.2:61054026-61054045 MS.gene70805:intron 30.0%
!!! ACTTTTCTGAAATTTGCCAA+GGG - chr1.2:61054909-61054928 MS.gene70805:CDS 30.0%
!!! GCTTTGTAACCTTTTTTTTG+TGG - chr1.2:61054173-61054192 MS.gene70805:intron 30.0%
AAAGTCAAGGAAGCAAATGA+AGG - chr1.2:61055064-61055083 MS.gene70805:intron 35.0%
AGTTATTTGTATTGCCTGCT+GGG - chr1.2:61054867-61054886 MS.gene70805:intron 35.0%
ATAGATCAAACCAAAACCAG+TGG + chr1.2:61054063-61054082 None:intergenic 35.0%
CAACATCAATGTTAACAACC+AGG + chr1.2:61054275-61054294 None:intergenic 35.0%
CAACTACATTAACAGGCAAA+AGG + chr1.2:61054937-61054956 None:intergenic 35.0%
CTGGTTGTTAACATTGATGT+TGG - chr1.2:61054273-61054292 MS.gene70805:intron 35.0%
TAGTTATTTGTATTGCCTGC+TGG - chr1.2:61054866-61054885 MS.gene70805:intron 35.0%
TTTCTCTTGGATAATTGGAC+TGG - chr1.2:61054151-61054170 MS.gene70805:intron 35.0%
! AAGTTCAGAACTCATTTTGG+AGG - chr1.2:61054029-61054048 MS.gene70805:intron 35.0%
! ACCCTAACAAGTCTGTATTT+TGG + chr1.2:61054116-61054135 None:intergenic 35.0%
! GTAGAATGGACTTGATACTA+AGG - chr1.2:61055037-61055056 MS.gene70805:intron 35.0%
! GTGTGTTTGTTAACAAGTGA+AGG - chr1.2:61054600-61054619 MS.gene70805:CDS 35.0%
!! ACATCAGTGTTTTTGCCAAA+TGG - chr1.2:61054301-61054320 MS.gene70805:intron 35.0%
!! CATTGTATTTTTGCAGACCA+AGG - chr1.2:61055205-61055224 MS.gene70805:CDS 35.0%
!!! CACTTTTCTGAAATTTGCCA+AGG - chr1.2:61054908-61054927 MS.gene70805:CDS 35.0%
!!! TGCACTTCCTTTTGATTTGA+CGG - chr1.2:61054526-61054545 MS.gene70805:intron 35.0%
AACACACTTATAACAGCACC+AGG + chr1.2:61054587-61054606 None:intergenic 40.0%
ACAAATTCAAACGTGAGCAC+TGG + chr1.2:61054773-61054792 None:intergenic 40.0%
GCTTTACCTTCTTAACTCCA+CGG + chr1.2:61055115-61055134 None:intergenic 40.0%
GTCATTGATGTTCTTCATCC+TGG - chr1.2:61053782-61053801 MS.gene70805:intron 40.0%
TCACGTTTGAATTTGTCCAG+TGG - chr1.2:61054777-61054796 MS.gene70805:intron 40.0%
TGATGTTCTTCATCCTGGAA+GGG - chr1.2:61053787-61053806 MS.gene70805:intron 40.0%
TGCAAGGATTTACGATGTGA+AGG - chr1.2:61053983-61054002 MS.gene70805:intron 40.0%
TGGCAAAAACACTGATGTTG+AGG + chr1.2:61054299-61054318 None:intergenic 40.0%
TTGATGTTCTTCATCCTGGA+AGG - chr1.2:61053786-61053805 MS.gene70805:intron 40.0%
! GGAAGTGCAAAGTCAAACAT+CGG + chr1.2:61054515-61054534 None:intergenic 40.0%
! TTATTTCTTCTTGCCAGCCT+TGG + chr1.2:61055243-61055262 None:intergenic 40.0%
!! CATGAACTTTCTGGTTCTGA+TGG + chr1.2:61053482-61053501 None:intergenic 40.0%
!! CTTTCTGGTTCTGATGGTAA+CGG + chr1.2:61053476-61053495 None:intergenic 40.0%
!! GTTTGTTGTGTTTGGTGTGA+AGG - chr1.2:61053941-61053960 MS.gene70805:intron 40.0%
!! TTCAGAACTCATTTTGGAGG+CGG - chr1.2:61054032-61054051 MS.gene70805:intron 40.0%
!!! GCACTTCCTTTTGATTTGAC+GGG - chr1.2:61054527-61054546 MS.gene70805:intron 40.0%
AGCTAAAGGAGAAGCTTGCA+AGG - chr1.2:61053967-61053986 MS.gene70805:intron 45.0%
CCAGAAAGTTCATGACCAAC+AGG - chr1.2:61053488-61053507 MS.gene70805:intron 45.0%
CCTGTTGGTCATGAACTTTC+TGG + chr1.2:61053491-61053510 None:intergenic 45.0%
GAAACTCACGAATTGCTTCC+TGG + chr1.2:61053521-61053540 None:intergenic 45.0%
GAAGATCCGTGGAGTTAAGA+AGG - chr1.2:61055106-61055125 MS.gene70805:intron 45.0%
TACGCTCCCGTCAAATCAAA+AGG + chr1.2:61054536-61054555 None:intergenic 45.0%
TGCAAGCTTCTCCTTTAGCT+CGG + chr1.2:61053967-61053986 None:intergenic 45.0%
TGGAAGGGCAAATGTTTCCA+AGG - chr1.2:61053802-61053821 MS.gene70805:intron 45.0%
TTGGAAACATTTGCCCTTCC+AGG + chr1.2:61053803-61053822 None:intergenic 45.0%
! TGATTTGACGGGAGCGTATT+TGG - chr1.2:61054538-61054557 MS.gene70805:intron 45.0%
!! GTATTTTTGCAGACCAAGGC+TGG - chr1.2:61055209-61055228 MS.gene70805:CDS 45.0%
AACAGGGCAAAGAAGATCCG+TGG - chr1.2:61055095-61055114 MS.gene70805:intron 50.0%
AAGTAGAACATGTGCCCAGC+AGG + chr1.2:61054884-61054903 None:intergenic 50.0%
ACTGACAGAACTGACACCAC+TGG + chr1.2:61054796-61054815 None:intergenic 50.0%
TAACAGGCAAAAGGAGCCCT+TGG + chr1.2:61054928-61054947 None:intergenic 50.0%
CTTCCTGGAGAGAAGCCTGT+TGG + chr1.2:61053506-61053525 None:intergenic 55.0%
TGACCAACAGGCTTCTCTCC+AGG - chr1.2:61053500-61053519 MS.gene70805:intron 55.0%
TGGTGTGAAGGCCGAGCTAA+AGG - chr1.2:61053953-61053972 MS.gene70805:intron 55.0%
CTTGGCAGCATCACCAGCCT+TGG + chr1.2:61055225-61055244 None:intergenic 60.0%
TTTGGAGGCGGCAAGTCCAC+TGG - chr1.2:61054044-61054063 MS.gene70805:intron 60.0%
! CAAGGCTGGTGATGCTGCCA+AGG - chr1.2:61055223-61055242 MS.gene70805:CDS 60.0%
!!! GGCGGCAAGTCCACTGGTTT+TGG - chr1.2:61054050-61054069 MS.gene70805:intron 60.0%
! GCTGGTGATGCTGCCAAGGC+TGG - chr1.2:61055227-61055246 MS.gene70805:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 61053460 61055262 61053460 ID=MS.gene70805
chr1.2 mRNA 61053460 61055262 61053460 ID=MS.gene70805.t1;Parent=MS.gene70805
chr1.2 exon 61055191 61055262 61055191 ID=MS.gene70805.t1.exon1;Parent=MS.gene70805.t1
chr1.2 CDS 61055191 61055262 61055191 ID=cds.MS.gene70805.t1;Parent=MS.gene70805.t1
chr1.2 exon 61054899 61054940 61054899 ID=MS.gene70805.t1.exon2;Parent=MS.gene70805.t1
chr1.2 CDS 61054899 61054940 61054899 ID=cds.MS.gene70805.t1;Parent=MS.gene70805.t1
chr1.2 exon 61054589 61054759 61054589 ID=MS.gene70805.t1.exon3;Parent=MS.gene70805.t1
chr1.2 CDS 61054589 61054759 61054589 ID=cds.MS.gene70805.t1;Parent=MS.gene70805.t1
chr1.2 exon 61053595 61053681 61053595 ID=MS.gene70805.t1.exon4;Parent=MS.gene70805.t1
chr1.2 CDS 61053595 61053681 61053595 ID=cds.MS.gene70805.t1;Parent=MS.gene70805.t1
chr1.2 exon 61053460 61053501 61053460 ID=MS.gene70805.t1.exon5;Parent=MS.gene70805.t1
chr1.2 CDS 61053460 61053501 61053460 ID=cds.MS.gene70805.t1;Parent=MS.gene70805.t1
Gene Sequence

>MS.gene70805

ATGGCAGACAAGGCCGTTACCATCAGAACCAGAAAGTTCATGACCAACAGGCTTCTCTCCAGGAAGCAATTCGTGAGTTTCCTTCTTCCCTTTCTTTATTAGATCTGTATACTCTTCTAATCTGTTATCGCTAATAGACTGTGTCATTTGAAACATTTTTTTCATTGTTTTGCTCAAATTTAGTAAGTTAACATTCAAACTTAATTATTTTAATAATAATTTTGATTTTTAATGTTGTAGTATGATTAGAATTAGTATGGATGATTTATGTCGAATTATTTTTAGGAGTTTCTGATCCGATTTTTTTTATTGTGTGATGAAGGTCATTGATGTTCTTCATCCTGGAAGGGCAAATGTTTCCAAGGTATTTGTTATTTGTATCTAATATGCTATGAATGTTAGATAGGTTTTGATGATTTATATTGTCTCAGAGGCTGAAACTTACGTTATATTATGGTGTATTGGTTGAAATTGATTTATCGTTTGTTGTGTTTGGTGTGAAGGCCGAGCTAAAGGAGAAGCTTGCAAGGATTTACGATGTGAAGGACCCAAACACTGTTTTTGTGTTCAAGTTCAGAACTCATTTTGGAGGCGGCAAGTCCACTGGTTTTGGTTTGATCTATGATTCTGTTGAGAATGCAAAGAAGTATGAACCAAAATACAGACTTGTTAGGGTGAGTTAATTCTGTCTTTTCTCTTGGATAATTGGACTGGCTTTGTAACCTTTTTTTTGTGGTTTATTTGTTGCGATCTTTGTATAAGTTAAGCTGAATATTATTGTTTTTTTTCTCTTTTGATTTAGTTTGCTATTTCCTGGTTGTTAACATTGATGTTGGCCTCAACATCAGTGTTTTTGCCAAATGGATTTGTTTTTTTTTTCGGAATTTGGAACAAGTTCTTCAAATGTGAAGCATGTCAATTGTAAATGATCAAATGATTTGCTTGATGTATCTTGATGTTCTACTTTCAATTGCATTGTACATCCTCATTTGCTTGTGAATTTAGTTCATGAATTTGCCTATTCATTTATTTTTTTCAAGTTTAACTCTGTTCCGATGTTTGACTTTGCACTTCCTTTTGATTTGACGGGAGCGTATTTGGTTTGCTATTTCTGTTAAATAGTTCCTGGTGCTGTTATAAGTGTGTTTGTTAACAAGTGAAGGGCTTATCCACCCTACATTTAATTTCAATCATTTTTTATCAAACTGTTAAAATTCTAGTGATGTGTTCTTTATTTTCTTATGTAGTTTAGAGATAATTTAGCTGGATTGTTTTTTCCCTATAATTATGTACTCTCTTGAAGAATTTCTCCAGTGCTCACGTTTGAATTTGTCCAGTGGTGTCAGTTCTGTCAGTTTTTAATCTTAAGAAATAAAATGATTTGCTTGTAGTGTTGTTATTGGTTATAGTTATTTGTATTGCCTGCTGGGCACATGTTCTACTTCACTCACTTTTCTGAAATTTGCCAAGGGCTCCTTTTGCCTGTTAATGTAGTTGATGAATCTGCATATTCATTTAAATTTGCAATTTGAACTCTGTTATGTTATTGAAAATTATAATTCATTTTCTTTTCTATTGTAGAATGGACTTGATACTAAGGTTGAAAAGTCAAGGAAGCAAATGAAGGAAAGAAAGAACAGGGCAAAGAAGATCCGTGGAGTTAAGAAGGTAAAGCATATGTTCTGTCTTGATAATTTTTCATTTTATACATTTTGCTTTGTAAAACTCTGAAGTATAATCTCTTTCATTGTATTTTTGCAGACCAAGGCTGGTGATGCTGCCAAGGCTGGCAAGAAGAAATAA

Protein sequence

>MS.gene70805.t1

MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK