Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68832.t1 | XP_003620949.2 | 98.7 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 9.00E-82 | 312.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68832.t1 | Q8S8N6 | 65.6 | 151 | 45 | 1 | 3 | 153 | 5 | 148 | 2.0e-53 | 209.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68832.t1 | B7FGW1 | 98.7 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 6.5e-82 | 312.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene68832 | 0.842453 | 2.56E-58 | -1.69E-46 |
| MS.gene051439 | MS.gene68832 | 0.808046 | 3.75E-50 | -1.69E-46 |
| MS.gene052169 | MS.gene68832 | 0.872236 | 3.71E-67 | -1.69E-46 |
| MS.gene052209 | MS.gene68832 | 0.836894 | 7.16E-57 | -1.69E-46 |
| MS.gene052378 | MS.gene68832 | 0.807698 | 4.45E-50 | -1.69E-46 |
| MS.gene053224 | MS.gene68832 | 0.802491 | 5.47E-49 | -1.69E-46 |
| MS.gene054662 | MS.gene68832 | 0.842517 | 2.46E-58 | -1.69E-46 |
| MS.gene054941 | MS.gene68832 | 0.848951 | 4.42E-60 | -1.69E-46 |
| MS.gene056387 | MS.gene68832 | -0.803781 | 2.96E-49 | -1.69E-46 |
| MS.gene05652 | MS.gene68832 | 0.833446 | 5.31E-56 | -1.69E-46 |
| MS.gene057367 | MS.gene68832 | 0.821233 | 4.50E-53 | -1.69E-46 |
| MS.gene057669 | MS.gene68832 | 0.828619 | 8.13E-55 | -1.69E-46 |
| MS.gene057988 | MS.gene68832 | 0.80886 | 2.52E-50 | -1.69E-46 |
| MS.gene057990 | MS.gene68832 | 0.821391 | 4.14E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68832.t1 | MTR_6g092790 | 98.693 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 2.22e-112 | 315 |
| MS.gene68832.t1 | MTR_8g101550 | 62.676 | 142 | 53 | 0 | 8 | 149 | 4 | 145 | 6.67e-65 | 195 |
| MS.gene68832.t1 | MTR_6g091720 | 45.122 | 82 | 35 | 2 | 35 | 116 | 30 | 101 | 9.35e-18 | 75.1 |
| MS.gene68832.t1 | MTR_6g091750 | 36.364 | 110 | 54 | 3 | 42 | 145 | 52 | 151 | 7.45e-17 | 73.2 |
| MS.gene68832.t1 | MTR_6g091700 | 42.697 | 89 | 41 | 2 | 3 | 91 | 1 | 79 | 4.23e-15 | 68.2 |
| MS.gene68832.t1 | MTR_6g091730 | 41.538 | 65 | 36 | 1 | 63 | 127 | 36 | 98 | 3.17e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68832.t1 | AT2G06925 | 65.563 | 151 | 45 | 1 | 3 | 153 | 5 | 148 | 1.71e-71 | 212 |
| MS.gene68832.t1 | AT2G06925 | 65.306 | 147 | 44 | 1 | 3 | 149 | 5 | 144 | 2.14e-68 | 204 |
| MS.gene68832.t1 | AT2G19690 | 54.839 | 62 | 27 | 1 | 35 | 96 | 23 | 83 | 2.98e-18 | 75.5 |
| MS.gene68832.t1 | AT2G19690 | 46.250 | 80 | 40 | 2 | 35 | 114 | 23 | 99 | 4.63e-18 | 75.9 |
| MS.gene68832.t1 | AT2G19690 | 46.250 | 80 | 40 | 2 | 35 | 114 | 23 | 99 | 5.39e-18 | 75.9 |
| MS.gene68832.t1 | AT4G29460 | 46.914 | 81 | 40 | 2 | 37 | 117 | 23 | 100 | 6.93e-18 | 76.6 |
| MS.gene68832.t1 | AT4G29470 | 42.268 | 97 | 52 | 3 | 21 | 117 | 8 | 100 | 5.65e-17 | 74.3 |
| MS.gene68832.t1 | AT4G29470 | 42.268 | 97 | 52 | 3 | 21 | 117 | 8 | 100 | 5.65e-17 | 74.3 |
Find 21 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATGGAAGCTGCTTTAC+TGG | 0.266457 | 6.1:+80090451 | MS.gene68832:CDS |
| CCATGCAGTCTATGAATGTT+TGG | 0.339978 | 6.1:-80090349 | None:intergenic |
| GTGCCTCCATTGTTGAGATA+TGG | 0.393871 | 6.1:+80089333 | MS.gene68832:intron |
| TGTGAGTCAAGTTTCTGTTC+AGG | 0.441225 | 6.1:+80089239 | MS.gene68832:CDS |
| CATGTGTTTCCTTTGAATGT+AGG | 0.457173 | 6.1:-80090395 | None:intergenic |
| TTTGGCTGCACTGCTGACTT+AGG | 0.479097 | 6.1:-80090331 | None:intergenic |
| CATGTTTATGCACTTAACAT+AGG | 0.509197 | 6.1:+80089071 | MS.gene68832:CDS |
| ATGGAAGCTGCTTTACTGGC+TGG | 0.510582 | 6.1:+80090455 | MS.gene68832:CDS |
| ACAAAGGCTCCTACATTCAA+AGG | 0.530546 | 6.1:+80090386 | MS.gene68832:CDS |
| TACTGTGGACTATTGTATAG+TGG | 0.545854 | 6.1:+80089360 | MS.gene68832:CDS |
| CTTCAATAACATCATCTGCT+TGG | 0.548099 | 6.1:-80090418 | None:intergenic |
| TGAAGTTATCAAGATTGTCA+TGG | 0.550055 | 6.1:+80090436 | MS.gene68832:CDS |
| CCAAACATTCATAGACTGCA+TGG | 0.586577 | 6.1:+80090349 | MS.gene68832:CDS |
| CTATTGTATAGTGGATGCCC+TGG | 0.587699 | 6.1:+80089369 | MS.gene68832:CDS |
| AACATAGGTGCTGAAACCAC+TGG | 0.598513 | 6.1:+80089086 | MS.gene68832:CDS |
| TACTTTCCATATCTCAACAA+TGG | 0.617587 | 6.1:-80089339 | None:intergenic |
| GTTAAGTGCATAAACATGAG+TGG | 0.652688 | 6.1:-80089066 | None:intergenic |
| AACTGAAACAGCTACTCCAG+TGG | 0.671963 | 6.1:-80089102 | None:intergenic |
| TTTCCATATCTCAACAATGG+AGG | 0.673712 | 6.1:-80089336 | None:intergenic |
| TTGAGATATGGAAAGTACTG+TGG | 0.689516 | 6.1:+80089345 | MS.gene68832:CDS |
| CAAGCATCTAGACCATCACA+AGG | 0.698237 | 6.1:-80089399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAATTTAACATATATATT+AGG | + | chr6.1:80089714-80089733 | MS.gene68832:intron | 10.0% |
| !!! | TTAATTAATATGTTTAGAAT+TGG | - | chr6.1:80089153-80089172 | None:intergenic | 10.0% |
| !! | AAATAAAATAAAGATATGGT+AGG | + | chr6.1:80089796-80089815 | MS.gene68832:intron | 15.0% |
| !! | ACACAAATAAAATAAAGATA+TGG | + | chr6.1:80089792-80089811 | MS.gene68832:intron | 15.0% |
| !!! | AAATTCTAAAATATGTTATC+AGG | - | chr6.1:80089647-80089666 | None:intergenic | 15.0% |
| !! | AAATGAAGTAATTAGATGAA+GGG | - | chr6.1:80089930-80089949 | None:intergenic | 20.0% |
| !! | ACTAGTAAATAGTATTAGTT+TGG | - | chr6.1:80090034-80090053 | None:intergenic | 20.0% |
| !! | ATTATCAAATGTAATGGTTA+TGG | - | chr6.1:80090076-80090095 | None:intergenic | 20.0% |
| !! | CATAAGATTAATAGTTACTA+AGG | - | chr6.1:80090140-80090159 | None:intergenic | 20.0% |
| !! | TATGGATAGTCAAAAAATTT+AGG | + | chr6.1:80089618-80089637 | MS.gene68832:intron | 20.0% |
| !! | TTATCAAATGTAATGGTTAT+GGG | - | chr6.1:80090075-80090094 | None:intergenic | 20.0% |
| !!! | ATCTAACATTGTTATTGATT+AGG | + | chr6.1:80089555-80089574 | MS.gene68832:intron | 20.0% |
| !!! | ATTGGTTAGTCTTTATTTAT+GGG | - | chr6.1:80089905-80089924 | None:intergenic | 20.0% |
| !!! | TTTTTAACCAGTATACTAAT+TGG | + | chr6.1:80089852-80089871 | MS.gene68832:intron | 20.0% |
| !!! | TTTTTTGCTATACTTTATGT+TGG | + | chr6.1:80090110-80090129 | MS.gene68832:intron | 20.0% |
| ! | ATTCAATCCTAAAAGCTATA+TGG | + | chr6.1:80089600-80089619 | MS.gene68832:intron | 25.0% |
| ! | GAAATGAAGTAATTAGATGA+AGG | - | chr6.1:80089931-80089950 | None:intergenic | 25.0% |
| ! | GAATCAGATGATACTAAAAA+TGG | - | chr6.1:80089529-80089548 | None:intergenic | 25.0% |
| ! | TAAAATAAAGATATGGTAGG+TGG | + | chr6.1:80089799-80089818 | MS.gene68832:intron | 25.0% |
| ! | TAGCAAAAAAAAATTAGTCC+TGG | - | chr6.1:80090100-80090119 | None:intergenic | 25.0% |
| ! | TATATGTGTCAATATCATGT+TGG | - | chr6.1:80090222-80090241 | None:intergenic | 25.0% |
| ! | TCATCTAATTACTTCATTTC+TGG | + | chr6.1:80089931-80089950 | MS.gene68832:intron | 25.0% |
| !!! | ATAGCTTTTAGGATTGAATT+AGG | - | chr6.1:80089599-80089618 | None:intergenic | 25.0% |
| !!! | CATTGGTTAGTCTTTATTTA+TGG | - | chr6.1:80089906-80089925 | None:intergenic | 25.0% |
| !!! | TTTTTGAAAATTGAGCTGAT+TGG | + | chr6.1:80089302-80089321 | MS.gene68832:intron | 25.0% |
| ATCAGTAAGTATTATTGCAG+TGG | + | chr6.1:80089744-80089763 | MS.gene68832:intron | 30.0% | |
| CATGTTTATGCACTTAACAT+AGG | + | chr6.1:80089071-80089090 | MS.gene68832:CDS | 30.0% | |
| CCTGGAATTATCAAATGTAA+TGG | - | chr6.1:80090082-80090101 | None:intergenic | 30.0% | |
| CTCTTTGCTCTACAAAAAAA+AGG | - | chr6.1:80089207-80089226 | None:intergenic | 30.0% | |
| GGAAAAATTTAGGAACACAA+AGG | + | chr6.1:80090370-80090389 | MS.gene68832:CDS | 30.0% | |
| TACTTTCCATATCTCAACAA+TGG | - | chr6.1:80089342-80089361 | None:intergenic | 30.0% | |
| TAGACTGCATGGAAAAATTT+AGG | + | chr6.1:80090360-80090379 | MS.gene68832:CDS | 30.0% | |
| TGAAGTTATCAAGATTGTCA+TGG | + | chr6.1:80090436-80090455 | MS.gene68832:CDS | 30.0% | |
| TGTCAATATCATGTTGGTAT+CGG | - | chr6.1:80090216-80090235 | None:intergenic | 30.0% | |
| ! | CCATTACATTTGATAATTCC+AGG | + | chr6.1:80090079-80090098 | MS.gene68832:intron | 30.0% |
| ! | TGACTATCCATATAGCTTTT+AGG | - | chr6.1:80089610-80089629 | None:intergenic | 30.0% |
| ! | TTTGCTATACTTTATGTTGG+TGG | + | chr6.1:80090113-80090132 | MS.gene68832:intron | 30.0% |
| !! | TATTTTTGACTGTGACACAT+TGG | - | chr6.1:80089440-80089459 | None:intergenic | 30.0% |
| AATCCTCTTAAGCATAGTCT+TGG | - | chr6.1:80089990-80090009 | None:intergenic | 35.0% | |
| AGCTAGCTAATGATTAATGC+TGG | + | chr6.1:80090289-80090308 | MS.gene68832:intron | 35.0% | |
| CATGTGTTTCCTTTGAATGT+AGG | - | chr6.1:80090398-80090417 | None:intergenic | 35.0% | |
| CTTCAATAACATCATCTGCT+TGG | - | chr6.1:80090421-80090440 | None:intergenic | 35.0% | |
| GTAATTAGATGAAGGGTCAT+TGG | - | chr6.1:80089923-80089942 | None:intergenic | 35.0% | |
| GTTAAGTGCATAAACATGAG+TGG | - | chr6.1:80089069-80089088 | None:intergenic | 35.0% | |
| TAAACTTCATAGCAGGTACA+AGG | - | chr6.1:80089878-80089897 | None:intergenic | 35.0% | |
| TACAAGGCCAATTAGTATAC+TGG | - | chr6.1:80089862-80089881 | None:intergenic | 35.0% | |
| TGTGTGTGTTTGATATCAAG+TGG | - | chr6.1:80089777-80089796 | None:intergenic | 35.0% | |
| TTTCAGACTGAATGTCTGTT+TGG | + | chr6.1:80090170-80090189 | MS.gene68832:intron | 35.0% | |
| TTTCCATATCTCAACAATGG+AGG | - | chr6.1:80089339-80089358 | None:intergenic | 35.0% | |
| ! | TACTGTGGACTATTGTATAG+TGG | + | chr6.1:80089360-80089379 | MS.gene68832:CDS | 35.0% |
| !! | TTGAGATATGGAAAGTACTG+TGG | + | chr6.1:80089345-80089364 | MS.gene68832:CDS | 35.0% |
| ACAAAGGCTCCTACATTCAA+AGG | + | chr6.1:80090386-80090405 | MS.gene68832:CDS | 40.0% | |
| ACTCCAAGACTATGCTTAAG+AGG | + | chr6.1:80089984-80090003 | MS.gene68832:intron | 40.0% | |
| CCAAACATTCATAGACTGCA+TGG | + | chr6.1:80090349-80090368 | MS.gene68832:CDS | 40.0% | |
| CCATGCAGTCTATGAATGTT+TGG | - | chr6.1:80090352-80090371 | None:intergenic | 40.0% | |
| GGGTACATAAACTTCATAGC+AGG | - | chr6.1:80089885-80089904 | None:intergenic | 40.0% | |
| GTTTGGTATCCTACACATGT+TGG | + | chr6.1:80090187-80090206 | MS.gene68832:intron | 40.0% | |
| TGTGAGTCAAGTTTCTGTTC+AGG | + | chr6.1:80089239-80089258 | MS.gene68832:CDS | 40.0% | |
| ! | CATCACAAGGTTTTTCACCA+GGG | - | chr6.1:80089389-80089408 | None:intergenic | 40.0% |
| ! | TAAGCATAGTCTTGGAGTGA+AGG | - | chr6.1:80089982-80090001 | None:intergenic | 40.0% |
| AACTGAAACAGCTACTCCAG+TGG | - | chr6.1:80089105-80089124 | None:intergenic | 45.0% | |
| AGTCTTGGAGTGAAGGAGAA+GGG | - | chr6.1:80089975-80089994 | None:intergenic | 45.0% | |
| CAAGCATCTAGACCATCACA+AGG | - | chr6.1:80089402-80089421 | None:intergenic | 45.0% | |
| CCTGGTGAAAAACCTTGTGA+TGG | + | chr6.1:80089387-80089406 | MS.gene68832:CDS | 45.0% | |
| CTTAGGTAGTCATCTGCAGA+AGG | - | chr6.1:80090317-80090336 | None:intergenic | 45.0% | |
| GTGCCTCCATTGTTGAGATA+TGG | + | chr6.1:80089333-80089352 | MS.gene68832:intron | 45.0% | |
| TATCGGACACCAACATGTGT+AGG | - | chr6.1:80090199-80090218 | None:intergenic | 45.0% | |
| ! | CCATCACAAGGTTTTTCACC+AGG | - | chr6.1:80089390-80089409 | None:intergenic | 45.0% |
| ! | TAGTCTTGGAGTGAAGGAGA+AGG | - | chr6.1:80089976-80089995 | None:intergenic | 45.0% |
| ! | TGTCATGGAAGCTGCTTTAC+TGG | + | chr6.1:80090451-80090470 | MS.gene68832:CDS | 45.0% |
| !! | AACATAGGTGCTGAAACCAC+TGG | + | chr6.1:80089086-80089105 | MS.gene68832:CDS | 45.0% |
| !! | CTATTGTATAGTGGATGCCC+TGG | + | chr6.1:80089369-80089388 | MS.gene68832:CDS | 45.0% |
| ! | ATGGAAGCTGCTTTACTGGC+TGG | + | chr6.1:80090455-80090474 | MS.gene68832:CDS | 50.0% |
| !! | TTTGGCTGCACTGCTGACTT+AGG | - | chr6.1:80090334-80090353 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 80089008 | 80090499 | 80089008 | ID=MS.gene68832 |
| chr6.1 | mRNA | 80089008 | 80090499 | 80089008 | ID=MS.gene68832.t1;Parent=MS.gene68832 |
| chr6.1 | exon | 80089008 | 80089124 | 80089008 | ID=MS.gene68832.t1.exon1;Parent=MS.gene68832.t1 |
| chr6.1 | CDS | 80089008 | 80089124 | 80089008 | ID=cds.MS.gene68832.t1;Parent=MS.gene68832.t1 |
| chr6.1 | exon | 80089218 | 80089260 | 80089218 | ID=MS.gene68832.t1.exon2;Parent=MS.gene68832.t1 |
| chr6.1 | CDS | 80089218 | 80089260 | 80089218 | ID=cds.MS.gene68832.t1;Parent=MS.gene68832.t1 |
| chr6.1 | exon | 80089334 | 80089459 | 80089334 | ID=MS.gene68832.t1.exon3;Parent=MS.gene68832.t1 |
| chr6.1 | CDS | 80089334 | 80089459 | 80089334 | ID=cds.MS.gene68832.t1;Parent=MS.gene68832.t1 |
| chr6.1 | exon | 80090324 | 80090499 | 80090324 | ID=MS.gene68832.t1.exon4;Parent=MS.gene68832.t1 |
| chr6.1 | CDS | 80090324 | 80090499 | 80090324 | ID=cds.MS.gene68832.t1;Parent=MS.gene68832.t1 |
| Gene Sequence |
| Protein sequence |
