AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69102


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69102.t1 MTR_3g089100 88.542 96 10 1 27 121 1 96 7.16e-31 115
MS.gene69102.t1 MTR_3g089100 88.542 96 10 1 27 121 1 96 7.45e-31 115
MS.gene69102.t1 MTR_3g089100 88.542 96 10 1 27 121 1 96 7.90e-31 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATAACTTCTTCTTCTT+TGG 0.231300 3.4:+96158860 None:intergenic
TTACCGGAGTTGTTGTTGTT+GGG 0.351319 3.4:+96158807 None:intergenic
ATTCTTTCTCAGCCTGATAA+TGG 0.358493 3.4:-96158650 MS.gene69102:intron
TTTACCGGAGTTGTTGTTGT+TGG 0.379968 3.4:+96158806 None:intergenic
AGAAGATGAAGGTCGTTTAC+CGG 0.381174 3.4:+96158791 None:intergenic
GATGAAGCAACACCATTATC+AGG 0.391396 3.4:+96158638 None:intergenic
GGTTCATCGATCTGAGCATT+AGG 0.402190 3.4:+96158554 None:intergenic
CGTTTAGATGAAGGAGAAGA+AGG 0.430320 3.4:+96158753 None:intergenic
GGGAGTTGCCAGTACCAAAC+GGG 0.430499 3.4:-96159535 MS.gene69102:CDS
GCAATCGCGGGAGTTGCATC+AGG 0.442296 3.4:+96158506 None:intergenic
TCCTTCATCTAAACGCCAAA+AGG 0.452837 3.4:-96158745 MS.gene69102:intron
TGAAAGGAGGGTACCCCGTT+TGG 0.464198 3.4:+96159521 None:intergenic
GAGGGTACCCCGTTTGGTAC+TGG 0.472129 3.4:+96159527 None:intergenic
AGCCAAAGAAGAAGAAGTTA+TGG 0.472596 3.4:-96158862 MS.gene69102:CDS
CAAGAACCGTCTGTCGATCC+CGG 0.476593 3.4:-96158590 MS.gene69102:CDS
GGTTCTTGATTTGAATTCTC+CGG 0.481752 3.4:+96158605 None:intergenic
TCATCATTAGCAGCTGCTAC+AGG 0.486403 3.4:+96158533 None:intergenic
TGATAATGGTGTTGCTTCAT+CGG 0.488569 3.4:-96158636 MS.gene69102:CDS
TACCGGAGTTGTTGTTGTTG+GGG 0.505915 3.4:+96158808 None:intergenic
AAGTCTCTAATCTCACTGAC+CGG 0.529132 3.4:-96159557 MS.gene69102:CDS
GGGGAGTTGCCAGTACCAAA+CGG 0.539647 3.4:-96159536 MS.gene69102:CDS
TAACGAACCATCAGCAATCG+CGG 0.554972 3.4:+96158493 None:intergenic
TTAGATGAAGGAGAAGAAGG+AGG 0.556358 3.4:+96158756 None:intergenic
GCAACTCCCGCGATTGCTGA+TGG 0.562807 3.4:-96158500 MS.gene69102:CDS
GTTTGGTACTGGCAACTCCC+CGG 0.566151 3.4:+96159538 None:intergenic
AGTCTCTAATCTCACTGACC+GGG 0.584559 3.4:-96159556 MS.gene69102:CDS
AACGAACCATCAGCAATCGC+GGG 0.588091 3.4:+96158494 None:intergenic
TGCTTCATCGGAGAAACCAC+CGG 0.588528 3.4:-96158624 MS.gene69102:CDS
AGAAGAAGGAGGTTTGTCAT+CGG 0.590143 3.4:+96158767 None:intergenic
TTGTCATCGGAAGAAGATGA+AGG 0.614507 3.4:+96158780 None:intergenic
TTCCCCAACAACAACAACTC+CGG 0.615482 3.4:-96158810 MS.gene69102:CDS
TCTTGATTTGAATTCTCCGG+TGG 0.625267 3.4:+96158608 None:intergenic
TCAACGAGACACAACAGTGG+TGG 0.655270 3.4:-96158837 MS.gene69102:CDS
AAGACCCATCTCATGCACAA+AGG 0.676403 3.4:-96159585 None:intergenic
GTCTCTAATCTCACTGACCG+GGG 0.681160 3.4:-96159555 MS.gene69102:CDS
GGAGTTGCCAGTACCAAACG+GGG 0.712771 3.4:-96159534 MS.gene69102:intron
GTTTCAACGAGACACAACAG+TGG 0.761613 3.4:-96158840 MS.gene69102:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTATTTAAATTAGTT+AGG + chr3.4:96158891-96158910 None:intergenic 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG + chr3.4:96158890-96158909 None:intergenic 10.0%
!! AGGAAGAAATTAAAAAAAAA+TGG - chr3.4:96159069-96159088 MS.gene69102:intron 15.0%
!! CCAATATATAATAAATAGAA+AGG + chr3.4:96158817-96158836 None:intergenic 15.0%
!! CCTTTCTATTTATTATATAT+TGG - chr3.4:96158814-96158833 MS.gene69102:CDS 15.0%
!! TTGGAGAAATTAAAAAAAAA+AGG - chr3.4:96159049-96159068 MS.gene69102:intron 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG + chr3.4:96158889-96158908 None:intergenic 15.0%
!! AATGGAAAAAGAAAATTGAA+AGG - chr3.4:96159087-96159106 MS.gene69102:intron 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG + chr3.4:96158888-96158907 None:intergenic 20.0%
! GAAAAGTTAATTCTTCTCAA+AGG + chr3.4:96158628-96158647 None:intergenic 25.0%
!! CTTTAAAAAAAATATCCACG+TGG + chr3.4:96159011-96159030 None:intergenic 25.0%
!!! GCTTGTTGATATTTTGAAAT+TGG - chr3.4:96159030-96159049 MS.gene69102:intron 25.0%
AACCATAACTTCTTCTTCTT+TGG + chr3.4:96159206-96159225 None:intergenic 30.0%
AAGAGAAATGAAGAAAAGAG+TGG + chr3.4:96159150-96159169 None:intergenic 30.0%
GAAAAAGAAAATTGAAAGGC+AGG - chr3.4:96159091-96159110 MS.gene69102:intron 30.0%
TGCCAAAGAATCAATACTTA+GGG + chr3.4:96158918-96158937 None:intergenic 30.0%
! AAAGAGAGAAAAAAGCGTAA+CGG + chr3.4:96159356-96159375 None:intergenic 30.0%
! CTCTAACTTTTCAAAAGTTG+CGG - chr3.4:96158856-96158875 MS.gene69102:CDS 30.0%
!! GCAACTTTTGAAAAGTTAGA+GGG + chr3.4:96158857-96158876 None:intergenic 30.0%
!!! TATTGATTCTTTGGCAGTTT+TGG - chr3.4:96158922-96158941 MS.gene69102:intron 30.0%
!!! TATTGGCTTAATTGCACTTT+TGG - chr3.4:96158831-96158850 MS.gene69102:CDS 30.0%
AATGAAGAAAAGAGTGGAGA+AGG + chr3.4:96159144-96159163 None:intergenic 35.0%
AGCCAAAGAAGAAGAAGTTA+TGG - chr3.4:96159201-96159220 MS.gene69102:intron 35.0%
ATCAGAGACCAAGTCAAAAA+TGG + chr3.4:96158957-96158976 None:intergenic 35.0%
ATTCTTTCTCAGCCTGATAA+TGG - chr3.4:96159413-96159432 MS.gene69102:intron 35.0%
CCAAAGAATCAATACTTAGG+GGG + chr3.4:96158916-96158935 None:intergenic 35.0%
CTGCCAAAGAATCAATACTT+AGG + chr3.4:96158919-96158938 None:intergenic 35.0%
GCCAAAGAATCAATACTTAG+GGG + chr3.4:96158917-96158936 None:intergenic 35.0%
! ATTCTTCTCAAAGGCTCTTT+TGG + chr3.4:96158619-96158638 None:intergenic 35.0%
! CTTTTCAAAAGTTGCGGTTA+TGG - chr3.4:96158862-96158881 MS.gene69102:CDS 35.0%
! GGTTCTTGATTTGAATTCTC+CGG + chr3.4:96159461-96159480 None:intergenic 35.0%
!! CGCAACTTTTGAAAAGTTAG+AGG + chr3.4:96158858-96158877 None:intergenic 35.0%
!! TGATAATGGTGTTGCTTCAT+CGG - chr3.4:96159427-96159446 MS.gene69102:intron 35.0%
!!! ATTAGGTTGAGCACATTTTC+CGG + chr3.4:96159495-96159514 None:intergenic 35.0%
AAAGAGTGGAGAAGGAACAA+TGG + chr3.4:96159136-96159155 None:intergenic 40.0%
AAGTCTCTAATCTCACTGAC+CGG - chr3.4:96158506-96158525 MS.gene69102:CDS 40.0%
ACCAAGTCAAAAATGGACCA+GGG + chr3.4:96158950-96158969 None:intergenic 40.0%
AGAAGAAGGAGGTTTGTCAT+CGG + chr3.4:96159299-96159318 None:intergenic 40.0%
AGAAGATGAAGGTCGTTTAC+CGG + chr3.4:96159275-96159294 None:intergenic 40.0%
ATGATCTCAAATGCTCTCTG+GGG + chr3.4:96158757-96158776 None:intergenic 40.0%
CATGATCTCAAATGCTCTCT+GGG + chr3.4:96158758-96158777 None:intergenic 40.0%
CGTTTAGATGAAGGAGAAGA+AGG + chr3.4:96159313-96159332 None:intergenic 40.0%
GATGAAGCAACACCATTATC+AGG + chr3.4:96159428-96159447 None:intergenic 40.0%
TCCTTCATCTAAACGCCAAA+AGG - chr3.4:96159318-96159337 MS.gene69102:intron 40.0%
TTAGATGAAGGAGAAGAAGG+AGG + chr3.4:96159310-96159329 None:intergenic 40.0%
TTGTCATCGGAAGAAGATGA+AGG + chr3.4:96159286-96159305 None:intergenic 40.0%
! ACCTTTTGGCGTTTAGATGA+AGG + chr3.4:96159322-96159341 None:intergenic 40.0%
! TCTTGATTTGAATTCTCCGG+TGG + chr3.4:96159458-96159477 None:intergenic 40.0%
! TTACCGGAGTTGTTGTTGTT+GGG + chr3.4:96159259-96159278 None:intergenic 40.0%
! TTCTCAAAGGCTCTTTTGGT+GGG + chr3.4:96158615-96158634 None:intergenic 40.0%
! TTTACCGGAGTTGTTGTTGT+TGG + chr3.4:96159260-96159279 None:intergenic 40.0%
!! CCCCCTAAGTATTGATTCTT+TGG - chr3.4:96158913-96158932 MS.gene69102:intron 40.0%
!! CGGATTGGAGAAAAACCTTT+TGG + chr3.4:96159336-96159355 None:intergenic 40.0%
!! GAGAAAAAAGCGTAACGGAT+TGG + chr3.4:96159351-96159370 None:intergenic 40.0%
!!! TTAGGTTGAGCACATTTTCC+GGG + chr3.4:96159494-96159513 None:intergenic 40.0%
AATGCTCTCTTGCTGAGAAG+AGG + chr3.4:96158660-96158679 None:intergenic 45.0%
AGTCTCTAATCTCACTGACC+GGG - chr3.4:96158507-96158526 MS.gene69102:CDS 45.0%
ATGCTCTCTTGCTGAGAAGA+GGG + chr3.4:96158659-96158678 None:intergenic 45.0%
CAAGTCAAAAATGGACCAGG+GGG + chr3.4:96158948-96158967 None:intergenic 45.0%
CCAAGTCAAAAATGGACCAG+GGG + chr3.4:96158949-96158968 None:intergenic 45.0%
CCATGATCTCAAATGCTCTC+TGG + chr3.4:96158759-96158778 None:intergenic 45.0%
CTGTTCTCCATGAGTCTGAA+AGG + chr3.4:96158561-96158580 None:intergenic 45.0%
GACCAAGTCAAAAATGGACC+AGG + chr3.4:96158951-96158970 None:intergenic 45.0%
GGTTCATCGATCTGAGCATT+AGG + chr3.4:96159512-96159531 None:intergenic 45.0%
GTTTCAACGAGACACAACAG+TGG - chr3.4:96159223-96159242 MS.gene69102:intron 45.0%
TAACGAACCATCAGCAATCG+CGG + chr3.4:96159573-96159592 None:intergenic 45.0%
TCATCATTAGCAGCTGCTAC+AGG + chr3.4:96159533-96159552 None:intergenic 45.0%
TCTCCATGAGTCTGAAAGGA+GGG + chr3.4:96158557-96158576 None:intergenic 45.0%
TGATCTCAAATGCTCTCTGG+GGG + chr3.4:96158756-96158775 None:intergenic 45.0%
TTCCCCAACAACAACAACTC+CGG - chr3.4:96159253-96159272 MS.gene69102:intron 45.0%
TTCTCCATGAGTCTGAAAGG+AGG + chr3.4:96158558-96158577 None:intergenic 45.0%
! CTTCTCAAAGGCTCTTTTGG+TGG + chr3.4:96158616-96158635 None:intergenic 45.0%
! TACCGGAGTTGTTGTTGTTG+GGG + chr3.4:96159258-96159277 None:intergenic 45.0%
! TCTCAAAGGCTCTTTTGGTG+GGG + chr3.4:96158614-96158633 None:intergenic 45.0%
!! CCAGAGAGCATTTGAGATCA+TGG - chr3.4:96158756-96158775 MS.gene69102:CDS 45.0%
!! CTTCTCAGCAAGAGAGCATT+TGG - chr3.4:96158660-96158679 MS.gene69102:intron 45.0%
AACGAACCATCAGCAATCGC+GGG + chr3.4:96159572-96159591 None:intergenic 50.0%
AGACACACACCCACAAAACC+CGG - chr3.4:96158730-96158749 MS.gene69102:intron 50.0%
GTACCCTCCTTTCAGACTCA+TGG - chr3.4:96158551-96158570 MS.gene69102:CDS 50.0%
GTCTCTAATCTCACTGACCG+GGG - chr3.4:96158508-96158527 MS.gene69102:CDS 50.0%
TCAACGAGACACAACAGTGG+TGG - chr3.4:96159226-96159245 MS.gene69102:intron 50.0%
TGCTGAGAAGAGGGAGATGT+GGG + chr3.4:96158650-96158669 None:intergenic 50.0%
TGCTTCATCGGAGAAACCAC+CGG - chr3.4:96159439-96159458 MS.gene69102:intron 50.0%
TGTGGGTGTGTGTCTTGAGT+GGG + chr3.4:96158725-96158744 None:intergenic 50.0%
TTGCTGAGAAGAGGGAGATG+TGG + chr3.4:96158651-96158670 None:intergenic 50.0%
TTGTGGGTGTGTGTCTTGAG+TGG + chr3.4:96158726-96158745 None:intergenic 50.0%
! CATTTTCCGGGATCGACAGA+CGG + chr3.4:96159482-96159501 None:intergenic 50.0%
! GCTCTTTTGGTGGGGATGTT+TGG + chr3.4:96158606-96158625 None:intergenic 50.0%
!! CCCCTGGTCCATTTTTGACT+TGG - chr3.4:96158946-96158965 MS.gene69102:intron 50.0%
!! TGACTTGGTCTCTGATGACG+TGG - chr3.4:96158961-96158980 MS.gene69102:intron 50.0%
!! TGGGGATGTTTGGTGTGTTG+CGG + chr3.4:96158596-96158615 None:intergenic 50.0%
CAAGAACCGTCTGTCGATCC+CGG - chr3.4:96159473-96159492 MS.gene69102:intron 55.0%
CACGTGGCACCAAACACAAG+GGG + chr3.4:96158995-96159014 None:intergenic 55.0%
CCACGTGGCACCAAACACAA+GGG + chr3.4:96158996-96159015 None:intergenic 55.0%
GCATCCAGACCCCTTGTGTT+TGG - chr3.4:96158983-96159002 MS.gene69102:intron 55.0%
GGAGTTGCCAGTACCAAACG+GGG - chr3.4:96158529-96158548 MS.gene69102:CDS 55.0%
GGGAGTTGCCAGTACCAAAC+GGG - chr3.4:96158528-96158547 MS.gene69102:CDS 55.0%
GGGGAGTTGCCAGTACCAAA+CGG - chr3.4:96158527-96158546 MS.gene69102:CDS 55.0%
GTGGGTGTGTGTCTTGAGTG+GGG + chr3.4:96158724-96158743 None:intergenic 55.0%
TCCACGTGGCACCAAACACA+AGG + chr3.4:96158997-96159016 None:intergenic 55.0%
TGGGTGTGTGTCTTGAGTGG+GGG + chr3.4:96158723-96158742 None:intergenic 55.0%
! GTCTTGAGTGGGGGGTTTTG+AGG + chr3.4:96158714-96158733 None:intergenic 55.0%
!! GGATGTTTGGTGTGTTGCGG+GGG + chr3.4:96158593-96158612 None:intergenic 55.0%
!! GGGATGTTTGGTGTGTTGCG+GGG + chr3.4:96158594-96158613 None:intergenic 55.0%
!! GGGGATGTTTGGTGTGTTGC+GGG + chr3.4:96158595-96158614 None:intergenic 55.0%
!! GTTTGGTACTGGCAACTCCC+CGG + chr3.4:96158528-96158547 None:intergenic 55.0%
!! TGAAAGGAGGGTACCCCGTT+TGG + chr3.4:96158545-96158564 None:intergenic 55.0%
GCAACTCCCGCGATTGCTGA+TGG - chr3.4:96159563-96159582 MS.gene69102:CDS 60.0%
GCAATCGCGGGAGTTGCATC+AGG + chr3.4:96159560-96159579 None:intergenic 60.0%
GGCACCAAACACAAGGGGTC+TGG + chr3.4:96158990-96159009 None:intergenic 60.0%
GGGTGTGTGTCTTGAGTGGG+GGG + chr3.4:96158722-96158741 None:intergenic 60.0%
! CTCAAATGCTCTCTGGGGGC+CGG + chr3.4:96158752-96158771 None:intergenic 60.0%
! TCAAATGCTCTCTGGGGGCC+GGG + chr3.4:96158751-96158770 None:intergenic 60.0%
!! CCCTTGTGTTTGGTGCCACG+TGG - chr3.4:96158993-96159012 MS.gene69102:intron 60.0%
!! CTTTGGCAGTTTTGGCCCCC+TGG - chr3.4:96158930-96158949 MS.gene69102:intron 60.0%
!! GAGGGTACCCCGTTTGGTAC+TGG + chr3.4:96158539-96158558 None:intergenic 60.0%
!! CTCTGGGGGCCGGGTTTTGT+GGG + chr3.4:96158742-96158761 None:intergenic 65.0%
!! TCTCTGGGGGCCGGGTTTTG+TGG + chr3.4:96158743-96158762 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 96158490 96159595 96158490 ID=MS.gene69102
chr3.4 mRNA 96158490 96159595 96158490 ID=MS.gene69102.t1;Parent=MS.gene69102
chr3.4 exon 96159535 96159595 96159535 ID=MS.gene69102.t1.exon1;Parent=MS.gene69102.t1
chr3.4 CDS 96159535 96159595 96159535 ID=cds.MS.gene69102.t1;Parent=MS.gene69102.t1
chr3.4 exon 96158746 96158882 96158746 ID=MS.gene69102.t1.exon2;Parent=MS.gene69102.t1
chr3.4 CDS 96158746 96158882 96158746 ID=cds.MS.gene69102.t1;Parent=MS.gene69102.t1
chr3.4 exon 96158490 96158660 96158490 ID=MS.gene69102.t1.exon3;Parent=MS.gene69102.t1
chr3.4 CDS 96158490 96158660 96158490 ID=cds.MS.gene69102.t1;Parent=MS.gene69102.t1
Gene Sequence

>MS.gene69102

ATGCACAAAGGAAGAAAAGTCTCTAATCTCACTGACCGGGGAGTTGCCAGTACCAAACGGGGTACCCTCCTTTCAGACTCATGGAGAACAGAGTTAAAATCCCCCGCAACACACCAAACATCCCCACCAAAAGAGCCTTTGAGAAGAATTAACTTTTCCCACATCTCCCTCTTCTCAGCAAGAGAGCATTTGGAATACACGTTCACAACAAAACAGTGACTCCTCAAAACCCCCCACTCAAGACACACACCCACAAAACCCGGCCCCCAGAGAGCATTTGAGATCATGGTTTTTATTTAGTAGTATTTAAGTTGAAATAAATTTCCTTTCTATTTATTATATATTGGCTTAATTGCACTTTTGGCCCTCTAACTTTTCAAAAGTTGCGGTTATGGCCCCCTAACTAATTTAAATACAAAATAGCCCCCTAAGTATTGATTCTTTGGCAGTTTTGGCCCCCTGGTCCATTTTTGACTTGGTCTCTGATGACGTGGCATCCAGACCCCTTGTGTTTGGTGCCACGTGGATATTTTTTTTAAAGCTTGTTGATATTTTGAAATTGGAGAAATTAAAAAAAAAAGGAAGAAATTAAAAAAAAATGGAAAAAGAAAATTGAAAGGCAGGTCACATGACTTCTTCTTCTCCATTGTTCCTTCTCCACTCTTTTCTTCATTTCTCTTTTCTCTTCTCTTTCTCTGTGTTTATCGTTGAAGCCAAAGAAGAAGAAGTTATGGTTTCAACGAGACACAACAGTGGTGGATCTTTCCCCAACAACAACAACTCCGGTAAACGACCTTCATCTTCTTCCGATGACAAACCTCCTTCTTCTCCTTCATCTAAACGCCAAAAGGTTTTTCTCCAATCCGTTACGCTTTTTTCTCTCTTTCATCTTTATTCGATTCAATTTCTTAATTTTGATTTTCATTCTTTCTCAGCCTGATAATGGTGTTGCTTCATCGGAGAAACCACCGGAGAATTCAAATCAAGAACCGTCTGTCGATCCCGGAAAATGTGCTCAACCTAATGCTCAGATCGATGAACCTGTAGCAGCTGCTAATGATGATAAACCTGATGCAACTCCCGCGATTGCTGATGGTTCGTTATAA

Protein sequence

>MS.gene69102.t1

MHKGRKVSNLTDRGVASTKRAKEEEVMVSTRHNSGGSFPNNNNSGKRPSSSSDDKPPSSPSSKRQKPDNGVASSEKPPENSNQEPSVDPGKCAQPNAQIDEPVAAANDDKPDATPAIADGSL