AlfalfaGEDB | MS.gene69346

M. sativa cultivar XinJiangDaYe / MS.gene69346

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69346.t1	XP_013462246.1	98.1	107	2	0	1	107	1	107	2.30E-47	198

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69346.t1	A0A072V3Q4	98.1	107	2	0	1	107	1	107	1.6e-47	198.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049983	MS.gene69346	-0.811699	6.14E-51	-1.69E-46
MS.gene050759	MS.gene69346	-0.817535	3.14E-52	-1.69E-46
MS.gene050962	MS.gene69346	-0.812985	3.22E-51	-1.69E-46
MS.gene051198	MS.gene69346	-0.800151	1.65E-48	-1.69E-46
MS.gene052674	MS.gene69346	-0.803163	3.97E-49	-1.69E-46
MS.gene052675	MS.gene69346	-0.807866	4.10E-50	-1.69E-46
MS.gene052677	MS.gene69346	-0.80949	1.84E-50	-1.69E-46
MS.gene056776	MS.gene69346	-0.805253	1.46E-49	-1.69E-46
MS.gene056779	MS.gene69346	-0.8025	5.45E-49	-1.69E-46
MS.gene060011	MS.gene69346	-0.807306	5.39E-50	-1.69E-46
MS.gene060850	MS.gene69346	-0.811058	8.47E-51	-1.69E-46
MS.gene060851	MS.gene69346	-0.811528	6.69E-51	-1.69E-46
MS.gene060852	MS.gene69346	-0.809896	1.51E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69346.t1	MTR_3g115060	98.131	107	2	0	1	107	1	107	4.64e-73	212

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69346.t1	AT1G20430	65.882	85	29	0	23	107	29	113	1.05e-33	113

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTTAAGCATGAAATCTT+TGG	0.222509	3.3:-93873615	MS.gene69346:CDS
CTCTTAAGCATGAAATCTTT+GGG	0.311239	3.3:-93873614	MS.gene69346:CDS
CCGAATGCCGCCGGTGCCGT+TGG	0.453325	3.3:-93873722	MS.gene69346:CDS
CCTCTTGGAGTCCATGGATT+CGG	0.472053	3.3:+93873582	None:intergenic
CCAACGGCACCGGCGGCATT+CGG	0.480360	3.3:+93873722	None:intergenic
TCAGATCATGAGTATCCTCT+TGG	0.496481	3.3:+93873567	None:intergenic
GAGTATCCTCTTGGAGTCCA+TGG	0.510166	3.3:+93873576	None:intergenic
ATTCGGTCGCACTGAGGAGG+AGG	0.524049	3.3:+93873739	None:intergenic
GAAGCTTCTCAAGATTCTAC+TGG	0.533479	3.3:-93873485	MS.gene69346:CDS
GGACAAAAGTACCGAATCCA+TGG	0.544794	3.3:-93873593	MS.gene69346:CDS
CTTCTTCGAACCAACGGCAC+CGG	0.551741	3.3:+93873712	None:intergenic
TAGCTTCTTCTTCGAACCAA+CGG	0.568765	3.3:+93873706	None:intergenic
CTTCGAACCAACGGCACCGG+CGG	0.570741	3.3:+93873715	None:intergenic
GGCATTCGGTCGCACTGAGG+AGG	0.571642	3.3:+93873736	None:intergenic
CACTGAGGAGGAGGAAGACA+TGG	0.572521	3.3:+93873748	None:intergenic
TCAGTGCGACCGAATGCCGC+CGG	0.579043	3.3:-93873731	MS.gene69346:CDS
AAGAGGATACTCATGATCTG+AGG	0.589972	3.3:-93873565	MS.gene69346:CDS
AGAGGATACTCATGATCTGA+GGG	0.604407	3.3:-93873564	MS.gene69346:CDS
CCGAATCCATGGACTCCAAG+AGG	0.665176	3.3:-93873582	MS.gene69346:CDS
GGCGGCATTCGGTCGCACTG+AGG	0.704327	3.3:+93873733	None:intergenic
ATTCATTGAAGAGACTACGA+AGG	0.718138	3.3:+93873447	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTCTTAAGCATGAAATCTTT+GGG	-	chr3.3:93873577-93873596	MS.gene69346:CDS	30.0%
	TCTCTTAAGCATGAAATCTT+TGG	-	chr3.3:93873576-93873595	MS.gene69346:CDS	30.0%
!	CTTGATGTTTTTCATACGAT+CGG	+	chr3.3:93873671-93873690	None:intergenic	30.0%
	ATTCATTGAAGAGACTACGA+AGG	+	chr3.3:93873747-93873766	None:intergenic	35.0%
!	ATTCAACTTTTCGCAATGAC+TGG	-	chr3.3:93873547-93873566	MS.gene69346:CDS	35.0%
	AAGAGGATACTCATGATCTG+AGG	-	chr3.3:93873626-93873645	MS.gene69346:CDS	40.0%
	AGAGGATACTCATGATCTGA+GGG	-	chr3.3:93873627-93873646	MS.gene69346:CDS	40.0%
	GAAGCTTCTCAAGATTCTAC+TGG	-	chr3.3:93873706-93873725	MS.gene69346:CDS	40.0%
	TAGCTTCTTCTTCGAACCAA+CGG	+	chr3.3:93873488-93873507	None:intergenic	40.0%
	TCAGATCATGAGTATCCTCT+TGG	+	chr3.3:93873627-93873646	None:intergenic	40.0%
!!	GGACAAAAGTACCGAATCCA+TGG	-	chr3.3:93873598-93873617	MS.gene69346:CDS	45.0%
	CCTCTTGGAGTCCATGGATT+CGG	+	chr3.3:93873612-93873631	None:intergenic	50.0%
	GAGTATCCTCTTGGAGTCCA+TGG	+	chr3.3:93873618-93873637	None:intergenic	50.0%
	CCGAATCCATGGACTCCAAG+AGG	-	chr3.3:93873609-93873628	MS.gene69346:CDS	55.0%
	CTTCTTCGAACCAACGGCAC+CGG	+	chr3.3:93873482-93873501	None:intergenic	55.0%
	ATTCGGTCGCACTGAGGAGG+AGG	+	chr3.3:93873455-93873474	None:intergenic	60.0%
	GGCATTCGGTCGCACTGAGG+AGG	+	chr3.3:93873458-93873477	None:intergenic	65.0%
	TCAGTGCGACCGAATGCCGC+CGG	-	chr3.3:93873460-93873479	MS.gene69346:CDS	65.0%
!	CTTCGAACCAACGGCACCGG+CGG	+	chr3.3:93873479-93873498	None:intergenic	65.0%
	GGCGGCATTCGGTCGCACTG+AGG	+	chr3.3:93873461-93873480	None:intergenic	70.0%
!!	CCAACGGCACCGGCGGCATT+CGG	+	chr3.3:93873472-93873491	None:intergenic	70.0%
!!	CCGAATGCCGCCGGTGCCGT+TGG	-	chr3.3:93873469-93873488	MS.gene69346:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	93873445	93873768	93873445	ID=MS.gene69346
chr3.3	mRNA	93873445	93873768	93873445	ID=MS.gene69346.t1;Parent=MS.gene69346
chr3.3	exon	93873445	93873768	93873445	ID=MS.gene69346.t1.exon1;Parent=MS.gene69346.t1
chr3.3	CDS	93873445	93873768	93873445	ID=cds.MS.gene69346.t1;Parent=MS.gene69346.t1

Gene Sequence	>MS.gene69346 ATGTCTTCCTCCTCCTCAGTGCGACCGAATGCCGCCGGTGCCGTTGGTTCGAAGAAGAAGCTAAGTTTCTTTGCGAACGCGATGAAACGAAAAGACAGCTTCATTCAACTTTTCGCAATGACTGGAATTCTTCTCTTAAGCATGAAATCTTTGGGACAAAAGTACCGAATCCATGGACTCCAAGAGGATACTCATGATCTGAGGGAAGAACATGATTCACTCACCGATCGTATGAAAAACATCAAGACTTCATTGCTTCACGAAGCTTCTCAAGATTCTACTGGTCTCTTCGCTTCTCGCCTTCGTAGTCTCTTCAATGAATAG
Protein sequence	>MS.gene69346.t1 MSSSSSVRPNAAGAVGSKKKLSFFANAMKRKDSFIQLFAMTGILLLSMKSLGQKYRIHGLQEDTHDLREEHDSLTDRMKNIKTSLLHEASQDSTGLFASRLRSLFNE