AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70238

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70238.t1 MTR_8g092870 97.136 419 11 1 1 419 1 418 0.0 818
MS.gene70238.t1 MTR_3g095730 64.789 426 136 6 1 419 1 419 0.0 535
MS.gene70238.t1 MTR_5g077510 29.263 434 261 19 8 419 33 442 4.15e-39 146
MS.gene70238.t1 MTR_5g015210 30.673 401 245 12 5 401 63 434 1.34e-38 144
MS.gene70238.t1 MTR_7g106340 30.073 409 251 12 8 397 6 398 1.55e-37 141
MS.gene70238.t1 MTR_6g071340 27.830 424 266 13 8 408 3 409 1.76e-37 141
MS.gene70238.t1 MTR_2g087350 28.607 402 253 9 8 397 4 383 3.71e-35 134
MS.gene70238.t1 MTR_5g048050 27.123 424 269 13 8 408 23 429 4.55e-34 132
MS.gene70238.t1 MTR_1g017770 27.873 409 242 13 10 401 45 417 6.25e-33 129
MS.gene70238.t1 MTR_1g056880 28.054 442 269 13 8 419 5 427 1.26e-32 128
MS.gene70238.t1 MTR_1g056870 27.602 442 271 13 8 419 5 427 1.84e-31 125
MS.gene70238.t1 MTR_3g065080 25.935 401 271 10 8 398 34 418 3.83e-30 121
MS.gene70238.t1 MTR_4g085720 28.288 403 260 11 5 399 9 390 3.49e-28 115
MS.gene70238.t1 MTR_1g056840 26.923 442 248 13 8 419 5 401 3.83e-28 115
MS.gene70238.t1 MTR_4g063800 26.952 397 255 12 9 396 254 624 1.00e-27 116
MS.gene70238.t1 MTR_7g059405 25.558 403 256 14 10 400 264 634 2.21e-26 112
MS.gene70238.t1 MTR_4g485520 26.359 368 229 12 2 354 97 437 9.35e-26 109
MS.gene70238.t1 MTR_4g051515 25.829 422 274 15 8 409 4 406 1.08e-25 108
MS.gene70238.t1 MTR_4g485520 26.230 366 222 12 7 354 270 605 1.34e-25 109
MS.gene70238.t1 MTR_3g096370 29.250 400 262 9 8 399 8 394 3.06e-25 107
MS.gene70238.t1 MTR_2g018010 26.942 412 260 12 5 399 287 674 4.40e-25 108
MS.gene70238.t1 MTR_1g056910 27.007 411 261 14 8 403 4 390 1.90e-24 104
MS.gene70238.t1 MTR_4g028960 26.764 411 258 14 8 399 278 664 6.41e-23 101
MS.gene70238.t1 MTR_1g094215 26.542 373 235 11 10 366 64 413 7.47e-21 94.7
MS.gene70238.t1 MTR_1g094215 26.542 373 235 11 10 366 247 596 1.81e-20 94.4
MS.gene70238.t1 MTR_7g116600 27.569 399 254 15 11 399 79 452 3.50e-20 92.8
MS.gene70238.t1 MTR_5g083030 25.722 381 222 15 6 354 289 640 2.59e-19 90.9
MS.gene70238.t1 MTR_3g078160 24.818 411 258 14 11 399 276 657 2.39e-18 87.8
MS.gene70238.t1 MTR_3g078160 24.818 411 258 14 11 399 276 657 2.68e-18 87.8
MS.gene70238.t1 MTR_4g107010 57.812 64 27 0 11 74 281 344 5.75e-18 86.7
MS.gene70238.t1 MTR_7g053260 25.187 401 268 11 8 400 76 452 4.31e-17 83.2
MS.gene70238.t1 MTR_3g085610 36.879 141 72 6 11 144 281 411 8.91e-17 83.2
MS.gene70238.t1 MTR_7g078330 24.363 353 236 10 8 354 272 599 1.05e-16 82.8
MS.gene70238.t1 MTR_5g034440 55.556 63 28 0 14 76 284 346 2.05e-16 82.0
MS.gene70238.t1 MTR_1g069845 43.478 69 39 0 8 76 122 190 9.38e-16 79.7
MS.gene70238.t1 MTR_7g097020 43.750 80 43 1 8 87 239 316 1.30e-15 79.3
MS.gene70238.t1 MTR_1g069845 43.478 69 39 0 8 76 239 307 1.56e-15 79.3
MS.gene70238.t1 MTR_1g069845 43.478 69 39 0 8 76 243 311 1.63e-15 79.0
MS.gene70238.t1 MTR_4g091880 46.377 69 36 1 10 77 5 73 1.08e-14 75.1
MS.gene70238.t1 MTR_4g091880 43.836 73 40 1 6 77 1 73 1.08e-14 75.5
MS.gene70238.t1 MTR_3g078160 50.000 64 32 0 11 74 276 339 1.60e-14 75.9
MS.gene70238.t1 MTR_2g011140 26.250 400 242 15 14 399 19 379 1.87e-13 72.0
MS.gene70238.t1 MTR_1g093965 50.877 57 28 0 23 79 1 57 4.82e-13 68.2
MS.gene70238.t1 MTR_1g079450 25.418 299 178 10 4 284 57 328 6.86e-13 70.5
MS.gene70238.t1 MTR_3g466220 41.667 72 42 0 8 79 210 281 1.97e-12 69.7
MS.gene70238.t1 MTR_3g466220 41.667 72 42 0 8 79 231 302 1.97e-12 69.7
MS.gene70238.t1 MTR_3g096370 28.514 249 124 10 8 243 8 215 2.65e-12 67.4
MS.gene70238.t1 MTR_1g093995 49.123 57 29 0 23 79 1 57 3.44e-12 65.5
MS.gene70238.t1 MTR_7g117890 44.615 65 36 0 14 78 259 323 3.52e-12 68.9
MS.gene70238.t1 MTR_8g077205 43.478 69 39 0 8 76 161 229 6.49e-12 67.8
MS.gene70238.t1 MTR_8g077205 43.478 69 39 0 8 76 273 341 7.51e-12 67.8
MS.gene70238.t1 MTR_8g080280 35.632 87 52 2 11 97 283 365 1.95e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70238.t1 AT5G64660 54.651 430 174 7 1 419 1 420 1.14e-145 421
MS.gene70238.t1 AT5G09800 53.791 422 179 7 1 419 1 409 4.65e-136 396
MS.gene70238.t1 AT3G18710 49.883 429 191 10 1 419 1 415 2.98e-130 382
MS.gene70238.t1 AT3G19380 29.082 392 248 10 5 384 9 382 5.00e-37 140
MS.gene70238.t1 AT5G65920 29.563 389 248 10 10 397 60 423 3.61e-35 135
MS.gene70238.t1 AT5G65920 29.563 389 248 10 10 397 60 423 3.61e-35 135
MS.gene70238.t1 AT2G35930 27.500 400 263 10 8 398 10 391 8.66e-35 133
MS.gene70238.t1 AT1G66160 26.485 404 262 13 4 398 27 404 4.75e-34 132
MS.gene70238.t1 AT3G49810 29.487 390 247 12 10 397 64 427 3.58e-33 130
MS.gene70238.t1 AT1G49780 27.700 426 284 11 5 419 9 421 4.97e-32 126
MS.gene70238.t1 AT3G52450 25.882 425 267 12 8 398 5 415 1.54e-30 122
MS.gene70238.t1 AT1G23030 25.432 405 263 14 8 405 239 611 1.77e-27 115
MS.gene70238.t1 AT2G28830 26.392 413 253 14 11 403 185 566 2.16e-27 114
MS.gene70238.t1 AT3G46510 25.791 411 240 15 10 396 256 625 1.11e-26 113
MS.gene70238.t1 AT5G37490 25.610 410 269 12 8 403 29 416 3.65e-26 110
MS.gene70238.t1 AT3G54850 27.195 353 228 9 10 354 248 579 5.92e-25 107
MS.gene70238.t1 AT1G71020 26.150 413 258 17 8 403 93 475 1.52e-24 105
MS.gene70238.t1 AT1G71020 26.150 413 258 17 8 403 241 623 3.55e-24 105
MS.gene70238.t1 AT1G66160 24.752 404 253 14 4 398 27 388 4.13e-24 103
MS.gene70238.t1 AT5G42340 26.786 280 181 8 8 283 288 547 7.66e-22 98.6
MS.gene70238.t1 AT3G11840 26.196 439 254 16 8 396 8 426 1.28e-21 97.1
MS.gene70238.t1 AT1G29340 25.592 422 270 15 5 408 300 695 2.12e-20 94.4
MS.gene70238.t1 AT5G01830 52.239 67 32 0 10 76 274 340 8.14e-19 89.4
MS.gene70238.t1 AT4G21350 24.745 392 253 13 6 389 1 358 1.66e-18 87.0
MS.gene70238.t1 AT3G54790 34.653 101 58 1 8 100 200 300 2.96e-17 84.7
MS.gene70238.t1 AT3G54790 34.653 101 58 1 8 100 236 336 4.03e-17 84.3
MS.gene70238.t1 AT3G54790 34.653 101 58 1 8 100 236 336 4.03e-17 84.3
MS.gene70238.t1 AT3G07360 24.586 362 235 14 8 355 72 409 1.79e-15 78.6
MS.gene70238.t1 AT1G67530 35.644 101 60 2 11 111 273 368 3.49e-13 71.6
MS.gene70238.t1 AT1G01660 42.466 73 42 0 4 76 491 563 3.53e-13 71.6
MS.gene70238.t1 AT1G01660 42.466 73 42 0 4 76 492 564 3.83e-13 71.6
MS.gene70238.t1 AT1G67530 35.644 101 60 2 11 111 273 368 4.75e-13 71.6
MS.gene70238.t1 AT1G67530 35.644 101 60 2 11 111 273 368 4.75e-13 71.6
MS.gene70238.t1 AT1G67530 35.644 101 60 2 11 111 273 368 4.75e-13 71.6
MS.gene70238.t1 AT1G10560 24.242 363 225 13 16 354 294 630 4.76e-13 71.6
MS.gene70238.t1 AT1G27910 40.230 87 48 2 11 97 280 362 6.14e-13 71.2
MS.gene70238.t1 AT5G18320 24.138 290 208 6 8 295 70 349 1.11e-12 69.7
MS.gene70238.t1 AT1G60190 23.810 357 239 10 14 355 282 620 1.21e-12 70.1
MS.gene70238.t1 AT1G24330 34.545 110 67 3 11 120 276 380 1.22e-12 70.5
MS.gene70238.t1 AT1G24330 34.545 110 67 3 11 120 276 380 1.22e-12 70.5
MS.gene70238.t1 AT5G18330 23.120 359 243 11 8 358 63 396 2.60e-12 68.6
MS.gene70238.t1 AT2G23140 44.444 63 35 0 14 76 237 299 1.42e-11 67.0
MS.gene70238.t1 AT2G23140 44.444 63 35 0 14 76 234 296 1.52e-11 67.0
MS.gene70238.t1 AT2G23140 44.444 63 35 0 14 76 234 296 1.52e-11 67.0
MS.gene70238.t1 AT2G23140 44.444 63 35 0 14 76 45 107 1.94e-11 66.2
MS.gene70238.t1 AT2G23140 44.444 63 35 0 14 76 45 107 1.94e-11 66.2

Find 83 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCATAATATGAGTAGTTT+TGG 0.313107 4.1:+8590075 None:intergenic
GGAGGGATTCGTGTGCGTTC+TGG 0.338842 4.1:-8590887 MS.gene70238:CDS
ACGAAAGTGCGTTCTCCATT+AGG 0.348641 4.1:+8590545 None:intergenic
GAAACGGAACAAGGCGAAAC+TGG 0.354390 4.1:-8590500 MS.gene70238:CDS
TCACCGTTCCGAGTTTATTC+CGG 0.356758 4.1:-8591284 MS.gene70238:CDS
AATCAAAATCAAAATCAAAA+TGG 0.359583 4.1:+8590055 None:intergenic
GGTTGTTATTGCGTTGGATT+TGG 0.360751 4.1:-8590806 MS.gene70238:CDS
TTCATGACGTCGAGGGAAAT+AGG 0.377245 4.1:+8591258 None:intergenic
AACTCGGTGACGCCGTTAGA+AGG 0.386589 4.1:+8590446 None:intergenic
GGAGAACGCACTTTCGTGTT+TGG 0.389136 4.1:-8590539 MS.gene70238:CDS
TCGGTGTATAACGGAGATTC+CGG 0.396983 4.1:+8591048 None:intergenic
GCACACGAATCCCTCCGTTT+TGG 0.409841 4.1:+8590894 None:intergenic
AGCGCCGTCGTGTGTGGTGG+CGG 0.420683 4.1:-8590341 MS.gene70238:CDS
CCAAGGATTTCGTTCCGAAC+CGG 0.425782 4.1:-8591128 MS.gene70238:CDS
GAACCGAATCGGACCAGATC+TGG 0.426727 4.1:+8591088 None:intergenic
GACTCGGTGGCGAACCGAAT+CGG 0.451818 4.1:+8591077 None:intergenic
TTATCTATTTCGTGATCAAA+AGG 0.458454 4.1:-8590245 MS.gene70238:CDS
CGCGTGCTCCGTCGCTGCGG+TGG 0.462131 4.1:+8590291 None:intergenic
TTGAGTCAGGTAGTGAGAAC+CGG 0.463717 4.1:-8590981 MS.gene70238:CDS
GAGTGTGGAATTGCTTGAAC+AGG 0.467131 4.1:-8590827 MS.gene70238:CDS
CATCCAGATCTGGTCCGATT+CGG 0.468264 4.1:-8591091 MS.gene70238:CDS
ATCCAACGCTGGCTCGATAA+CGG 0.470884 4.1:-8591189 MS.gene70238:CDS
ATTAGGTTCTGATCTTTCTC+CGG 0.473675 4.1:+8590562 None:intergenic
CCGGTTCGGAACGAAATCCT+TGG 0.487110 4.1:+8591128 None:intergenic
AGCCTCTGTAACGTCCGGTT+CGG 0.488322 4.1:+8591114 None:intergenic
GGAGAAAGATCAGAACCTAA+TGG 0.501306 4.1:-8590560 MS.gene70238:CDS
TACAGAGGCTCATCCAGATC+TGG 0.511051 4.1:-8591101 MS.gene70238:CDS
TAGCAATCTCAACGTCGAAA+CGG 0.512266 4.1:-8590516 MS.gene70238:CDS
GGTGTTGAAACTTATAGAAA+CGG 0.512499 4.1:-8590401 MS.gene70238:CDS
ACGGTGTCGTCGACAAAGGA+AGG 0.515605 4.1:-8590382 MS.gene70238:CDS
TACCGTTATCGAGCCAGCGT+TGG 0.518220 4.1:+8591187 None:intergenic
TTTCTTGATAATGTTGATGG+AGG 0.529867 4.1:-8590859 MS.gene70238:CDS
CTCAACGTCGAAACGGAACA+AGG 0.536307 4.1:-8590509 MS.gene70238:CDS
AGAAACGGTGTCGTCGACAA+AGG 0.541994 4.1:-8590386 MS.gene70238:CDS
GGATGAGCCTCTGTAACGTC+CGG 0.544595 4.1:+8591109 None:intergenic
TCAACAGACTCGGTGTATAA+CGG 0.556543 4.1:+8591039 None:intergenic
TGAACAGGTTGTTATTGCGT+TGG 0.556947 4.1:-8590812 MS.gene70238:CDS
TGAAGAATCTGCATAGTGGC+GGG 0.558784 4.1:+8591153 None:intergenic
AGTCGCTTGTGAAAATTGTA+CGG 0.565379 4.1:-8590954 MS.gene70238:CDS
GAGATTCCGGCGAGTCGACT+CGG 0.566820 4.1:+8591061 None:intergenic
GGTCTTCTCGAACCTTCTAA+CGG 0.571682 4.1:-8590458 MS.gene70238:CDS
CCTCGACGTCATGAAATCTC+CGG 0.572480 4.1:-8591250 MS.gene70238:CDS
GAAAGTATCCACCGCAGCGA+CGG 0.573216 4.1:-8590299 MS.gene70238:CDS
GAGTTTATGTGTTTCTGTGA+CGG 0.574001 4.1:-8590428 MS.gene70238:CDS
ACTCCGGTGCAGAGACTCAC+CGG 0.580529 4.1:+8591231 None:intergenic
GGTGTTGAAATTCATAGCGG+TGG 0.581421 4.1:-8590653 MS.gene70238:CDS
CGGAGATTTCATGACGTCGA+GGG 0.581997 4.1:+8591251 None:intergenic
ATGCGAAGCGCCGTCGTGTG+TGG 0.582842 4.1:-8590347 MS.gene70238:CDS
GTCGACAAAGGAAGGGAGGT+CGG 0.586477 4.1:-8590374 MS.gene70238:CDS
TGGAGGAGTTGAACAGAGTG+TGG 0.586843 4.1:-8590842 MS.gene70238:CDS
CGAAGCGCCGTCGTGTGTGG+TGG 0.591962 4.1:-8590344 MS.gene70238:CDS
GGTTCATCTCGGAGCAGTGA+AGG 0.592190 4.1:-8590479 MS.gene70238:CDS
GAAGCTGTTACGAAAGCGAA+TGG 0.592982 4.1:-8590217 MS.gene70238:CDS
ACAGACATTTGACGAACTTG+TGG 0.593505 4.1:+8590154 None:intergenic
AAGGCGAAACTGGTTCATCT+CGG 0.598473 4.1:-8590490 MS.gene70238:CDS
TCGCCACCGAGTCGACTCGC+CGG 0.598739 4.1:-8591067 MS.gene70238:CDS
GGTTTGAAGAATCTGCATAG+TGG 0.603561 4.1:+8591149 None:intergenic
TTGGATACTTGAACGGTCGT+AGG 0.604139 4.1:+8591206 None:intergenic
ATGTGTTTCTGTGACGGAGA+AGG 0.608428 4.1:-8590422 MS.gene70238:CDS
TCTCCGGTGAGTCTCTGCAC+CGG 0.610333 4.1:-8591234 MS.gene70238:CDS
TTGATTCTTGTTCTTCAACA+AGG 0.611383 4.1:-8590706 MS.gene70238:CDS
TTGAAGAATCTGCATAGTGG+CGG 0.623643 4.1:+8591152 None:intergenic
CACCGCAGCGACGGAGCACG+CGG 0.624475 4.1:-8590290 MS.gene70238:CDS
AACCGCGTGCTCCGTCGCTG+CGG 0.626410 4.1:+8590288 None:intergenic
TATGCAGATTCTTCAAACCA+AGG 0.633645 4.1:-8591145 MS.gene70238:CDS
TTCCGAACCGGACGTTACAG+AGG 0.635065 4.1:-8591116 MS.gene70238:CDS
ATAGGACACCGGAATAAACT+CGG 0.640762 4.1:+8591276 None:intergenic
ATCACGTCTATCAACAGACT+CGG 0.644157 4.1:+8591029 None:intergenic
GGTGGCGGCGATAGTTAACA+AGG 0.648696 4.1:-8590326 MS.gene70238:CDS
TGAACGGTCGTAGGTAACTC+CGG 0.649804 4.1:+8591215 None:intergenic
GTCACAGAAACACATAAACT+CGG 0.652220 4.1:+8590430 None:intergenic
GCCAGCGTTGGATACTTGAA+CGG 0.652606 4.1:+8591199 None:intergenic
ACCGTTCAAGTATCCAACGC+TGG 0.658519 4.1:-8591200 MS.gene70238:CDS
ATCGCCGCCACCACACACGA+CGG 0.660437 4.1:+8590337 None:intergenic
CGGTGTCGTCGACAAAGGAA+GGG 0.661079 4.1:-8590381 MS.gene70238:CDS
ACACCGGAATAAACTCGGAA+CGG 0.672519 4.1:+8591281 None:intergenic
CCGGAGATTTCATGACGTCG+AGG 0.678266 4.1:+8591250 None:intergenic
AGCACGCGGTTACAATACTG+TGG 0.681709 4.1:-8590276 MS.gene70238:CDS
ATTCCGGCGAGTCGACTCGG+TGG 0.696812 4.1:+8591064 None:intergenic
TGTCGTCGACAAAGGAAGGG+AGG 0.709533 4.1:-8590378 MS.gene70238:CDS
ATTGCTCAATCAAATCACGC+CGG 0.719116 4.1:-8590581 MS.gene70238:CDS
CGTCGAGGGAAATAGGACAC+CGG 0.731507 4.1:+8591265 None:intergenic
TAGGGTGTTGAAATTCATAG+CGG 0.751361 4.1:-8590656 MS.gene70238:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATCTATTTCGTGATCAAA+AGG - chr4.1:8591130-8591149 MS.gene70238:CDS 25.0%
!!! AGTTTTCTTGATAATGTTGA+TGG - chr4.1:8590513-8590532 MS.gene70238:CDS 25.0%
TTGATTCTTGTTCTTCAACA+AGG - chr4.1:8590669-8590688 MS.gene70238:CDS 30.0%
TTGCGAATCAAAAATTGCAT+CGG - chr4.1:8590695-8590714 MS.gene70238:CDS 30.0%
! GGTGTTGAAACTTATAGAAA+CGG - chr4.1:8590974-8590993 MS.gene70238:CDS 30.0%
! TGAAGAGAATTGTGTTTTTC+TGG - chr4.1:8590458-8590477 MS.gene70238:CDS 30.0%
! TGGCATAATATGAGTAGTTT+TGG + chr4.1:8591303-8591322 None:intergenic 30.0%
!! AATCAAAAATTGCATCGGTT+AGG - chr4.1:8590700-8590719 MS.gene70238:CDS 30.0%
!! ATCAAAAATTGCATCGGTTA+GGG - chr4.1:8590701-8590720 MS.gene70238:CDS 30.0%
!! TTTCTTGATAATGTTGATGG+AGG - chr4.1:8590516-8590535 MS.gene70238:CDS 30.0%
AGTCGCTTGTGAAAATTGTA+CGG - chr4.1:8590421-8590440 MS.gene70238:CDS 35.0%
CTTCTAGTAGCTGAAAAAGA+AGG - chr4.1:8590759-8590778 MS.gene70238:CDS 35.0%
GTCACAGAAACACATAAACT+CGG + chr4.1:8590948-8590967 None:intergenic 35.0%
TATGCAGATTCTTCAAACCA+AGG - chr4.1:8590230-8590249 MS.gene70238:CDS 35.0%
! ATTAGGTTCTGATCTTTCTC+CGG + chr4.1:8590816-8590835 None:intergenic 35.0%
! GAGTTTATGTGTTTCTGTGA+CGG - chr4.1:8590947-8590966 MS.gene70238:CDS 35.0%
!! ATCGATTTTGAAGAGCAAGA+AGG - chr4.1:8590617-8590636 MS.gene70238:CDS 35.0%
!! TAGGGTGTTGAAATTCATAG+CGG - chr4.1:8590719-8590738 MS.gene70238:CDS 35.0%
!! TTGTGTTTTTCTGGCCAAAA+CGG - chr4.1:8590467-8590486 MS.gene70238:CDS 35.0%
ACAGACATTTGACGAACTTG+TGG + chr4.1:8591224-8591243 None:intergenic 40.0%
ATAGGACACCGGAATAAACT+CGG + chr4.1:8590102-8590121 None:intergenic 40.0%
ATCACGTCTATCAACAGACT+CGG + chr4.1:8590349-8590368 None:intergenic 40.0%
ATTGCTCAATCAAATCACGC+CGG - chr4.1:8590794-8590813 MS.gene70238:CDS 40.0%
GGAGAAAGATCAGAACCTAA+TGG - chr4.1:8590815-8590834 MS.gene70238:CDS 40.0%
GGTTTGAAGAATCTGCATAG+TGG + chr4.1:8590229-8590248 None:intergenic 40.0%
TAGCAATCTCAACGTCGAAA+CGG - chr4.1:8590859-8590878 MS.gene70238:CDS 40.0%
TTGAAGAATCTGCATAGTGG+CGG + chr4.1:8590226-8590245 None:intergenic 40.0%
! GGTTGTTATTGCGTTGGATT+TGG - chr4.1:8590569-8590588 MS.gene70238:CDS 40.0%
! TGAACAGGTTGTTATTGCGT+TGG - chr4.1:8590563-8590582 MS.gene70238:CDS 40.0%
!! TCAACAGACTCGGTGTATAA+CGG + chr4.1:8590339-8590358 None:intergenic 40.0%
!!! GCTATCACTGATTTTGAGTC+AGG - chr4.1:8590381-8590400 MS.gene70238:CDS 40.0%
ACACCGGAATAAACTCGGAA+CGG + chr4.1:8590097-8590116 None:intergenic 45.0%
GAAGCTGTTACGAAAGCGAA+TGG - chr4.1:8591158-8591177 MS.gene70238:CDS 45.0%
GAGTGTGGAATTGCTTGAAC+AGG - chr4.1:8590548-8590567 MS.gene70238:CDS 45.0%
GGTCTTCTCGAACCTTCTAA+CGG - chr4.1:8590917-8590936 MS.gene70238:CDS 45.0%
TCACCGTTCCGAGTTTATTC+CGG - chr4.1:8590091-8590110 MS.gene70238:CDS 45.0%
TGAAGAATCTGCATAGTGGC+GGG + chr4.1:8590225-8590244 None:intergenic 45.0%
TTCATGACGTCGAGGGAAAT+AGG + chr4.1:8590120-8590139 None:intergenic 45.0%
TTGAGTCAGGTAGTGAGAAC+CGG - chr4.1:8590394-8590413 MS.gene70238:CDS 45.0%
TTGGATACTTGAACGGTCGT+AGG + chr4.1:8590172-8590191 None:intergenic 45.0%
! AAGGCGAAACTGGTTCATCT+CGG - chr4.1:8590885-8590904 MS.gene70238:CDS 45.0%
! ACGAAAGTGCGTTCTCCATT+AGG + chr4.1:8590833-8590852 None:intergenic 45.0%
! ATGTGTTTCTGTGACGGAGA+AGG - chr4.1:8590953-8590972 MS.gene70238:CDS 45.0%
! GGTGTTGAAATTCATAGCGG+TGG - chr4.1:8590722-8590741 MS.gene70238:CDS 45.0%
! TCGGTGTATAACGGAGATTC+CGG + chr4.1:8590330-8590349 None:intergenic 45.0%
! TTTTCACAAGCGACTCGAAC+CGG + chr4.1:8590416-8590435 None:intergenic 45.0%
!! GTTTTTCTGGCCAAAACGGA+GGG - chr4.1:8590471-8590490 MS.gene70238:CDS 45.0%
!! TGTTTTTCTGGCCAAAACGG+AGG - chr4.1:8590470-8590489 MS.gene70238:CDS 45.0%
ACCGTTCAAGTATCCAACGC+TGG - chr4.1:8590175-8590194 MS.gene70238:CDS 50.0%
AGCACGCGGTTACAATACTG+TGG - chr4.1:8591099-8591118 MS.gene70238:CDS 50.0%
CATCCAGATCTGGTCCGATT+CGG - chr4.1:8590284-8590303 MS.gene70238:CDS 50.0%
CCAAGGATTTCGTTCCGAAC+CGG - chr4.1:8590247-8590266 MS.gene70238:CDS 50.0%
CCTCGACGTCATGAAATCTC+CGG - chr4.1:8590125-8590144 MS.gene70238:CDS 50.0%
CGGAGATTTCATGACGTCGA+GGG + chr4.1:8590127-8590146 None:intergenic 50.0%
CTCAACGTCGAAACGGAACA+AGG - chr4.1:8590866-8590885 MS.gene70238:CDS 50.0%
GAAACGGAACAAGGCGAAAC+TGG - chr4.1:8590875-8590894 MS.gene70238:CDS 50.0%
GGAGAACGCACTTTCGTGTT+TGG - chr4.1:8590836-8590855 MS.gene70238:CDS 50.0%
TACAGAGGCTCATCCAGATC+TGG - chr4.1:8590274-8590293 MS.gene70238:CDS 50.0%
TGAACGGTCGTAGGTAACTC+CGG + chr4.1:8590163-8590182 None:intergenic 50.0%
TGGAGGAGTTGAACAGAGTG+TGG - chr4.1:8590533-8590552 MS.gene70238:CDS 50.0%
! GCCAGCGTTGGATACTTGAA+CGG + chr4.1:8590179-8590198 None:intergenic 50.0%
!! AGAAACGGTGTCGTCGACAA+AGG - chr4.1:8590989-8591008 MS.gene70238:CDS 50.0%
!! ATCCAACGCTGGCTCGATAA+CGG - chr4.1:8590186-8590205 MS.gene70238:CDS 50.0%
AGCCTCTGTAACGTCCGGTT+CGG + chr4.1:8590264-8590283 None:intergenic 55.0%
CCGGAGATTTCATGACGTCG+AGG + chr4.1:8590128-8590147 None:intergenic 55.0%
CGTCGAGGGAAATAGGACAC+CGG + chr4.1:8590113-8590132 None:intergenic 55.0%
GAACCGAATCGGACCAGATC+TGG + chr4.1:8590290-8590309 None:intergenic 55.0%
GGATGAGCCTCTGTAACGTC+CGG + chr4.1:8590269-8590288 None:intergenic 55.0%
GGTTCATCTCGGAGCAGTGA+AGG - chr4.1:8590896-8590915 MS.gene70238:CDS 55.0%
GTCGACAAAGGAAGGGAGGT+CGG - chr4.1:8591001-8591020 MS.gene70238:CDS 55.0%
TACCGTTATCGAGCCAGCGT+TGG + chr4.1:8590191-8590210 None:intergenic 55.0%
TGTCGTCGACAAAGGAAGGG+AGG - chr4.1:8590997-8591016 MS.gene70238:CDS 55.0%
TTCCGAACCGGACGTTACAG+AGG - chr4.1:8590259-8590278 MS.gene70238:CDS 55.0%
! AACTCGGTGACGCCGTTAGA+AGG + chr4.1:8590932-8590951 None:intergenic 55.0%
! GCACACGAATCCCTCCGTTT+TGG + chr4.1:8590484-8590503 None:intergenic 55.0%
! GGTGGCGGCGATAGTTAACA+AGG - chr4.1:8591049-8591068 MS.gene70238:CDS 55.0%
!! ACGGTGTCGTCGACAAAGGA+AGG - chr4.1:8590993-8591012 MS.gene70238:CDS 55.0%
!! CCGGTTCGGAACGAAATCCT+TGG + chr4.1:8590250-8590269 None:intergenic 55.0%
!! CGGTGTCGTCGACAAAGGAA+GGG - chr4.1:8590994-8591013 MS.gene70238:CDS 55.0%
!! GAAAGTATCCACCGCAGCGA+CGG - chr4.1:8591076-8591095 MS.gene70238:CDS 55.0%
ACTCCGGTGCAGAGACTCAC+CGG + chr4.1:8590147-8590166 None:intergenic 60.0%
GAGATTCCGGCGAGTCGACT+CGG + chr4.1:8590317-8590336 None:intergenic 60.0%
GGAGGGATTCGTGTGCGTTC+TGG - chr4.1:8590488-8590507 MS.gene70238:CDS 60.0%
TCTCCGGTGAGTCTCTGCAC+CGG - chr4.1:8590141-8590160 MS.gene70238:CDS 60.0%
!! GACTCGGTGGCGAACCGAAT+CGG + chr4.1:8590301-8590320 None:intergenic 60.0%
ATCGCCGCCACCACACACGA+CGG + chr4.1:8591041-8591060 None:intergenic 65.0%
!! ATGCGAAGCGCCGTCGTGTG+TGG - chr4.1:8591028-8591047 MS.gene70238:CDS 65.0%
!! ATTCCGGCGAGTCGACTCGG+TGG + chr4.1:8590314-8590333 None:intergenic 65.0%
AACCGCGTGCTCCGTCGCTG+CGG + chr4.1:8591090-8591109 None:intergenic 70.0%
AGCGCCGTCGTGTGTGGTGG+CGG - chr4.1:8591034-8591053 MS.gene70238:CDS 70.0%
!! CGAAGCGCCGTCGTGTGTGG+TGG - chr4.1:8591031-8591050 MS.gene70238:CDS 70.0%
!! TCGCCACCGAGTCGACTCGC+CGG - chr4.1:8590308-8590327 MS.gene70238:CDS 70.0%
CACCGCAGCGACGGAGCACG+CGG - chr4.1:8591085-8591104 MS.gene70238:CDS 75.0%
! CGCGTGCTCCGTCGCTGCGG+TGG + chr4.1:8591087-8591106 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8590069 8591328 8590069 ID=MS.gene70238
chr4.1 mRNA 8590069 8591328 8590069 ID=MS.gene70238.t1;Parent=MS.gene70238
chr4.1 exon 8590069 8591328 8590069 ID=MS.gene70238.t1.exon1;Parent=MS.gene70238.t1
chr4.1 CDS 8590069 8591328 8590069 ID=cds.MS.gene70238.t1;Parent=MS.gene70238.t1
Gene Sequence

>MS.gene70238

ATGGTAAGAGACGATTTATGCATCACCGTTCCGAGTTTATTCCGGTGTCCTATTTCCCTCGACGTCATGAAATCTCCGGTGAGTCTCTGCACCGGAGTTACCTACGACCGTTCAAGTATCCAACGCTGGCTCGATAACGGTAACAACACTTGTCCCGCCACTATGCAGATTCTTCAAACCAAGGATTTCGTTCCGAACCGGACGTTACAGAGGCTCATCCAGATCTGGTCCGATTCGGTTCGCCACCGAGTCGACTCGCCGGAATCTCCGTTATACACCGAGTCTGTTGATAGACGTGATCAATTACTTGTTGCTATCACTGATTTTGAGTCAGGTAGTGAGAACCGGTTCGAGTCGCTTGTGAAAATTGTACGGTTCGCGAAAGATTCTGAAGAGAATTGTGTTTTTCTGGCCAAAACGGAGGGATTCGTGTGCGTTCTGGTAAGTTTTCTTGATAATGTTGATGGAGGAGTTGAACAGAGTGTGGAATTGCTTGAACAGGTTGTTATTGCGTTGGATTTGGTTCTATGTAAAATTGAGAATCGCGAATCGATTTTGAAGAGCAAGAAGGAGAACGAAAGTAAGAGTATACTAGATTCTTTGATTCTTGTTCTTCAACAAGGAAGTTGCGAATCAAAAATTGCATCGGTTAGGGTGTTGAAATTCATAGCGGTGGACACAGAATCGAAGCTTCTAGTAGCTGAAAAAGAAGGTTTACTATCAGAATTGCTCAATCAAATCACGCCGGAGAAAGATCAGAACCTAATGGAGAACGCACTTTCGTGTTTGGTAGCAATCTCAACGTCGAAACGGAACAAGGCGAAACTGGTTCATCTCGGAGCAGTGAAGGTCTTCTCGAACCTTCTAACGGCGTCACCGAGTTTATGTGTTTCTGTGACGGAGAAGGTGTTGAAACTTATAGAAACGGTGTCGTCGACAAAGGAAGGGAGGTCGGAGATATGCGAAGCGCCGTCGTGTGTGGTGGCGGCGATAGTTAACAAGGTTTTGAAAGTATCCACCGCAGCGACGGAGCACGCGGTTACAATACTGTGGAGTGTGTGTTATCTATTTCGTGATCAAAAGGCACAAGAAGCTGTTACGAAAGCGAATGGATTAACGAAGATTTTGCTTCTGATGCAAAGTAATTGCTCGCCACAAGTTCGTCAAATGTCTGTAGATTTGCTTAAGATTTTTCGTGTTAATTCTAAGTCTTGTCTTTCTTCGTATGATACCAAAACTACTCATATTATGCCATTTTGA
Protein sequence

>MS.gene70238.t1

MVRDDLCITVPSLFRCPISLDVMKSPVSLCTGVTYDRSSIQRWLDNGNNTCPATMQILQTKDFVPNRTLQRLIQIWSDSVRHRVDSPESPLYTESVDRRDQLLVAITDFESGSENRFESLVKIVRFAKDSEENCVFLAKTEGFVCVLVSFLDNVDGGVEQSVELLEQVVIALDLVLCKIENRESILKSKKENESKSILDSLILVLQQGSCESKIASVRVLKFIAVDTESKLLVAEKEGLLSELLNQITPEKDQNLMENALSCLVAISTSKRNKAKLVHLGAVKVFSNLLTASPSLCVSVTEKVLKLIETVSSTKEGRSEICEAPSCVVAAIVNKVLKVSTAATEHAVTILWSVCYLFRDQKAQEAVTKANGLTKILLLMQSNCSPQVRQMSVDLLKIFRVNSKSCLSSYDTKTTHIMPF