AlfalfaGEDB | MS.gene055861

M. sativa cultivar XinJiangDaYe / MS.gene055861

KEGG: -
GO: -
PFAM: PF16166.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055861.t1	XP_013465032.1	99.3	144	1	0	1	144	129	272	7.70E-75	289.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055861.t1	Q9ZQZ9	53.1	143	67	0	1	143	140	282	1.7e-43	176.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055861.t1	A0A072VB93	99.3	144	1	0	1	144	129	272	5.5e-75	289.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050408	MS.gene055861	-0.811997	5.29E-51	-1.69E-46
MS.gene050807	MS.gene055861	-0.806298	8.80E-50	-1.69E-46
MS.gene052665	MS.gene055861	-0.848569	5.64E-60	-1.69E-46
MS.gene055465	MS.gene055861	-0.834953	2.22E-56	-1.69E-46
MS.gene055466	MS.gene055861	-0.817084	3.96E-52	-1.69E-46
MS.gene055468	MS.gene055861	-0.817726	2.84E-52	-1.69E-46
MS.gene055469	MS.gene055861	-0.843051	1.78E-58	-1.69E-46
MS.gene055861	MS.gene058087	-0.805258	1.46E-49	-1.69E-46
MS.gene055861	MS.gene06052	-0.806842	6.76E-50	-1.69E-46
MS.gene055861	MS.gene065908	-0.866543	2.64E-65	-1.69E-46
MS.gene055861	MS.gene066896	-0.803202	3.90E-49	-1.69E-46
MS.gene055861	MS.gene067234	-0.829689	4.47E-55	-1.69E-46
MS.gene055861	MS.gene070339	-0.804899	1.73E-49	-1.69E-46
MS.gene055861	MS.gene07047	-0.804726	1.88E-49	-1.69E-46
MS.gene055861	MS.gene07050	-0.811119	8.21E-51	-1.69E-46
MS.gene055861	MS.gene074519	-0.805966	1.03E-49	-1.69E-46
MS.gene055861	MS.gene21960	-0.801758	7.74E-49	-1.69E-46
MS.gene055861	MS.gene23105	-0.803589	3.24E-49	-1.69E-46
MS.gene055861	MS.gene27700	-0.802028	6.81E-49	-1.69E-46
MS.gene055861	MS.gene27918	-0.835618	1.51E-56	-1.69E-46
MS.gene055861	MS.gene31667	-0.80361	3.21E-49	-1.69E-46
MS.gene055861	MS.gene32555	-0.814204	1.74E-51	-1.69E-46
MS.gene055861	MS.gene35631	-0.813021	3.16E-51	-1.69E-46
MS.gene055861	MS.gene41380	-0.803011	4.27E-49	-1.69E-46
MS.gene055861	MS.gene41427	-0.801399	9.17E-49	-1.69E-46
MS.gene055861	MS.gene44001	-0.801985	6.95E-49	-1.69E-46
MS.gene055861	MS.gene47875	-0.835318	1.80E-56	-1.69E-46
MS.gene055861	MS.gene48814	-0.823331	1.47E-53	-1.69E-46
MS.gene055861	MS.gene49467	-0.80206	6.71E-49	-1.69E-46
MS.gene055861	MS.gene50163	-0.812682	3.75E-51	-1.69E-46
MS.gene055861	MS.gene55778	-0.803614	3.20E-49	-1.69E-46
MS.gene055861	MS.gene56748	-0.806132	9.54E-50	-1.69E-46
MS.gene055861	MS.gene58093	-0.813363	2.66E-51	-1.69E-46
MS.gene055861	MS.gene62682	-0.853802	1.88E-61	-1.69E-46
MS.gene055861	MS.gene64671	-0.818996	1.46E-52	-1.69E-46
MS.gene055861	MS.gene70238	-0.816294	5.96E-52	-1.69E-46
MS.gene055861	MS.gene78251	0.805114	1.56E-49	-1.69E-46
MS.gene055861	MS.gene78252	0.80943	1.90E-50	-1.69E-46
MS.gene055861	MS.gene96598	-0.848472	6.00E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055861.t1	MTR_2g090045	99.306	144	1	0	1	144	129	272	1.28e-102	295
MS.gene055861.t1	MTR_5g024800	93.750	144	9	0	1	144	92	235	6.66e-96	276
MS.gene055861.t1	MTR_2g090035	81.250	144	21	1	1	144	72	209	1.32e-78	231
MS.gene055861.t1	MTR_4g011960	32.824	131	85	1	2	132	124	251	3.41e-23	91.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055861.t1	AT4G03320	53.147	143	67	0	1	143	140	282	5.88e-57	179
MS.gene055861.t1	AT1G04940	41.221	131	74	1	2	132	143	270	1.07e-32	116
MS.gene055861.t1	AT1G04945	43.860	114	61	1	2	115	143	253	8.47e-30	113

Find 45 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTATATTATACTGCTATT+TGG	0.188855	2.1:-11393155	MS.gene055861:CDS
GAACACTTGGGATGTATTAT+TGG	0.195680	2.1:-11392997	MS.gene055861:CDS
TCTATCAGATGTGCTCTTCT+TGG	0.261813	2.1:-11392935	MS.gene055861:CDS
CTCTTGACTACTTCCTAAAA+TGG	0.273468	2.1:+11392836	None:intergenic
CTTCATTCCCTACTTAATAT+AGG	0.308831	2.1:-11392872	MS.gene055861:CDS
AAAGCTGATTCGCTAATAAC+AGG	0.313142	2.1:+11392896	None:intergenic
GGCAATCGGTTGACAGCTCC+TGG	0.313670	2.1:+11393187	None:intergenic
GCCAAATAGCAGTATAATAT+AGG	0.316868	2.1:+11393154	None:intergenic
TACATGGAATCTGAAGATAA+TGG	0.338807	2.1:+11393099	None:intergenic
ACATGGAATCTGAAGATAAT+GGG	0.352918	2.1:+11393100	None:intergenic
AACACTTGGGATGTATTATT+GGG	0.376072	2.1:-11392996	MS.gene055861:CDS
CCAAATAGCAGTATAATATA+GGG	0.392964	2.1:+11393155	None:intergenic
CTTCAGATTCCATGTAATGA+TGG	0.402408	2.1:-11393092	MS.gene055861:CDS
AAATTGCTCTGCAGATTGTA+TGG	0.403836	2.1:-11393057	MS.gene055861:CDS
ATACTGCTATTTGGCTATCA+TGG	0.434050	2.1:-11393146	MS.gene055861:CDS
CTGTCAACCGATTGCCAACC+TGG	0.447918	2.1:-11393180	MS.gene055861:CDS
TGTAATGATGGGAATGCTAC+TGG	0.470169	2.1:-11393080	MS.gene055861:CDS
CATTCCCTACTTAATATAGG+AGG	0.477614	2.1:-11392869	MS.gene055861:CDS
CATGCCACTGATACACTTCA+AGG	0.480381	2.1:-11393020	MS.gene055861:CDS
CTATATTAAGTAGGGAATGA+AGG	0.486900	2.1:+11392873	None:intergenic
ATACACTTCAAGGGAACACT+TGG	0.502149	2.1:-11393010	MS.gene055861:CDS
GGAACCCTCCTATATTAAGT+AGG	0.502372	2.1:+11392864	None:intergenic
GCATTGCAGATGTCTGCTGC+TGG	0.526281	2.1:-11393271	None:intergenic
CTACTTCCTAAAATGGCCGT+TGG	0.536876	2.1:+11392843	None:intergenic
CTTGGGATGTATTATTGGGC+TGG	0.536917	2.1:-11392992	MS.gene055861:CDS
TTATTGGGCTGGTGTGGCGT+TGG	0.539601	2.1:-11392981	MS.gene055861:CDS
ATTCCCTACTTAATATAGGA+GGG	0.550198	2.1:-11392868	MS.gene055861:CDS
TTCAGATTCCATGTAATGAT+GGG	0.550501	2.1:-11393091	MS.gene055861:CDS
CTATTTGGCTATCATGGTAG+TGG	0.557828	2.1:-11393140	MS.gene055861:CDS
TAGGGAACCAGGTTGGCAAT+CGG	0.558555	2.1:+11393173	None:intergenic
GAACCCTCCTATATTAAGTA+GGG	0.566246	2.1:+11392865	None:intergenic
AATTTGAATTTATCAAGCAA+AGG	0.581284	2.1:+11393235	None:intergenic
CATTGCAGATGTCTGCTGCT+GGG	0.582116	2.1:-11393270	None:intergenic
GTAGCATTCCCATCATTACA+TGG	0.585539	2.1:+11393083	None:intergenic
TTAATATAGGAGGGTTCCAA+CGG	0.593163	2.1:-11392859	MS.gene055861:CDS
GCAGTATAATATAGGGAACC+AGG	0.596497	2.1:+11393162	None:intergenic
ATGCCACTGATACACTTCAA+GGG	0.597572	2.1:-11393019	MS.gene055861:CDS
GATGTATTATTGGGCTGGTG+TGG	0.606994	2.1:-11392987	MS.gene055861:CDS
AATTGCTCTGCAGATTGTAT+GGG	0.609591	2.1:-11393056	MS.gene055861:CDS
GCAATCGGTTGACAGCTCCT+GGG	0.611407	2.1:+11393188	None:intergenic
CATGGAATCTGAAGATAATG+GGG	0.614103	2.1:+11393101	None:intergenic
TATAATATAGGGAACCAGGT+TGG	0.639459	2.1:+11393166	None:intergenic
AAGCTGATTCGCTAATAACA+GGG	0.643323	2.1:+11392897	None:intergenic
GTTCCCTTGAAGTGTATCAG+TGG	0.656964	2.1:+11393016	None:intergenic
TACACTTCAAGGGAACACTT+GGG	0.657791	2.1:-11393009	MS.gene055861:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTGAATTTATCAAGCAA+AGG	+	chr2.1:11392880-11392899	None:intergenic	20.0%
!	CCAAATAGCAGTATAATATA+GGG	+	chr2.1:11392960-11392979	None:intergenic	25.0%
	AACACTTGGGATGTATTATT+GGG	-	chr2.1:11393116-11393135	MS.gene055861:CDS	30.0%
	ACATGGAATCTGAAGATAAT+GGG	+	chr2.1:11393015-11393034	None:intergenic	30.0%
	ATTCCCTACTTAATATAGGA+GGG	-	chr2.1:11393244-11393263	MS.gene055861:CDS	30.0%
	CCCTATATTATACTGCTATT+TGG	-	chr2.1:11392957-11392976	MS.gene055861:CDS	30.0%
	CTATATTAAGTAGGGAATGA+AGG	+	chr2.1:11393242-11393261	None:intergenic	30.0%
	CTTCATTCCCTACTTAATAT+AGG	-	chr2.1:11393240-11393259	MS.gene055861:CDS	30.0%
	GCCAAATAGCAGTATAATAT+AGG	+	chr2.1:11392961-11392980	None:intergenic	30.0%
	TACATGGAATCTGAAGATAA+TGG	+	chr2.1:11393016-11393035	None:intergenic	30.0%
	TTCAGATTCCATGTAATGAT+GGG	-	chr2.1:11393021-11393040	MS.gene055861:CDS	30.0%
	AAATTGCTCTGCAGATTGTA+TGG	-	chr2.1:11393055-11393074	MS.gene055861:CDS	35.0%
	AATTGCTCTGCAGATTGTAT+GGG	-	chr2.1:11393056-11393075	MS.gene055861:CDS	35.0%
	CATGGAATCTGAAGATAATG+GGG	+	chr2.1:11393014-11393033	None:intergenic	35.0%
	CATTCCCTACTTAATATAGG+AGG	-	chr2.1:11393243-11393262	MS.gene055861:CDS	35.0%
	CTTCAGATTCCATGTAATGA+TGG	-	chr2.1:11393020-11393039	MS.gene055861:CDS	35.0%
	GAACACTTGGGATGTATTAT+TGG	-	chr2.1:11393115-11393134	MS.gene055861:CDS	35.0%
	GAACCCTCCTATATTAAGTA+GGG	+	chr2.1:11393250-11393269	None:intergenic	35.0%
	TATAATATAGGGAACCAGGT+TGG	+	chr2.1:11392949-11392968	None:intergenic	35.0%
	TTAATATAGGAGGGTTCCAA+CGG	-	chr2.1:11393253-11393272	MS.gene055861:CDS	35.0%
!	AATGTGATCTTTTACATCCC+AGG	-	chr2.1:11392907-11392926	MS.gene055861:CDS	35.0%
!	ATACTGCTATTTGGCTATCA+TGG	-	chr2.1:11392966-11392985	MS.gene055861:CDS	35.0%
!!	AAAGCTGATTCGCTAATAAC+AGG	+	chr2.1:11393219-11393238	None:intergenic	35.0%
!!	AAGCTGATTCGCTAATAACA+GGG	+	chr2.1:11393218-11393237	None:intergenic	35.0%
!!	CATGGTAGTGGTGAAAAATA+GGG	-	chr2.1:11392984-11393003	MS.gene055861:CDS	35.0%
!!	TCATGGTAGTGGTGAAAAAT+AGG	-	chr2.1:11392983-11393002	MS.gene055861:CDS	35.0%
	ATACACTTCAAGGGAACACT+TGG	-	chr2.1:11393102-11393121	MS.gene055861:CDS	40.0%
	ATGCCACTGATACACTTCAA+GGG	-	chr2.1:11393093-11393112	MS.gene055861:CDS	40.0%
	CTATTTGGCTATCATGGTAG+TGG	-	chr2.1:11392972-11392991	MS.gene055861:CDS	40.0%
	GCAGTATAATATAGGGAACC+AGG	+	chr2.1:11392953-11392972	None:intergenic	40.0%
	GGAACCCTCCTATATTAAGT+AGG	+	chr2.1:11393251-11393270	None:intergenic	40.0%
	GTAGCATTCCCATCATTACA+TGG	+	chr2.1:11393032-11393051	None:intergenic	40.0%
	TACACTTCAAGGGAACACTT+GGG	-	chr2.1:11393103-11393122	MS.gene055861:CDS	40.0%
	TCTATCAGATGTGCTCTTCT+TGG	-	chr2.1:11393177-11393196	MS.gene055861:CDS	40.0%
!	TGTAATGATGGGAATGCTAC+TGG	-	chr2.1:11393032-11393051	MS.gene055861:CDS	40.0%
	CATGCCACTGATACACTTCA+AGG	-	chr2.1:11393092-11393111	MS.gene055861:CDS	45.0%
	CTTGGGATGTATTATTGGGC+TGG	-	chr2.1:11393120-11393139	MS.gene055861:CDS	45.0%
	GTTCCCTTGAAGTGTATCAG+TGG	+	chr2.1:11393099-11393118	None:intergenic	45.0%
!!	GATGTATTATTGGGCTGGTG+TGG	-	chr2.1:11393125-11393144	MS.gene055861:CDS	45.0%
!	TAGGGAACCAGGTTGGCAAT+CGG	+	chr2.1:11392942-11392961	None:intergenic	50.0%
	CTGTCAACCGATTGCCAACC+TGG	-	chr2.1:11392932-11392951	MS.gene055861:CDS	55.0%
	GCAATCGGTTGACAGCTCCT+GGG	+	chr2.1:11392927-11392946	None:intergenic	55.0%
!!	TTATTGGGCTGGTGTGGCGT+TGG	-	chr2.1:11393131-11393150	MS.gene055861:CDS	55.0%
	GGCAATCGGTTGACAGCTCC+TGG	+	chr2.1:11392928-11392947	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	11392850	11393284	11392850	ID=MS.gene055861
chr2.1	mRNA	11392850	11393284	11392850	ID=MS.gene055861.t1;Parent=MS.gene055861
chr2.1	exon	11392850	11393284	11392850	ID=MS.gene055861.t1.exon1;Parent=MS.gene055861.t1
chr2.1	CDS	11392850	11393284	11392850	ID=cds.MS.gene055861.t1;Parent=MS.gene055861.t1

Gene Sequence

>MS.gene055861

ATGTCTGCTGCTGGGTATTATCTAGAACCTTTGCTTGATAAATTCAAATTATTTGAAAATGTGATCTTTTACATCCCAGGAGCTGTCAACCGATTGCCAACCTGGTTCCCTATATTATACTGCTATTTGGCTATCATGGTAGTGGTGAAAAATAGGGACTTCCCCATTATCTTCAGATTCCATGTAATGATGGGAATGCTACTGGAAATTGCTCTGCAGATTGTATGGGTTGCAAGCAATTTCATGCCACTGATACACTTCAAGGGAACACTTGGGATGTATTATTGGGCTGGTGTGGCGTTGGCATATATTGTCATAATCATGCATTCTATCAGATGTGCTCTTCTTGGTACATTTTCGAACATCCCTGTTATTAGCGAATCAGCTTTCCTTCATTCCCTACTTAATATAGGAGGGTTCCAACGGCCATTTTAG

Protein sequence

>MS.gene055861.t1

MSAAGYYLEPLLDKFKLFENVIFYIPGAVNRLPTWFPILYCYLAIMVVVKNRDFPIIFRFHVMMGMLLEIALQIVWVASNFMPLIHFKGTLGMYYWAGVALAYIVIIMHSIRCALLGTFSNIPVISESAFLHSLLNIGGFQRPF