AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71443.t1 MTR_1g075440 98.214 112 2 0 1 112 1 112 1.46e-74 231
MS.gene71443.t1 MTR_1g041815 83.784 111 17 1 1 111 1 110 2.82e-61 197
MS.gene71443.t1 MTR_1g041835 81.982 111 19 1 1 111 1 110 1.25e-60 195
MS.gene71443.t1 MTR_1g042310 84.314 102 16 0 10 111 8 109 1.28e-58 190
MS.gene71443.t1 MTR_1g041875 75.000 112 27 1 1 112 3 113 9.77e-57 184
MS.gene71443.t1 MTR_7g098160 74.038 104 27 0 8 111 13 116 5.13e-55 181
MS.gene71443.t1 MTR_7g098180 70.270 111 33 0 1 111 4 114 4.18e-54 178
MS.gene71443.t1 MTR_7g098150 72.115 104 29 0 8 111 13 116 9.21e-54 175
MS.gene71443.t1 MTR_7g098090 72.115 104 29 0 8 111 13 116 1.33e-53 177
MS.gene71443.t1 MTR_7g098220 66.364 110 35 1 2 111 6 113 2.86e-50 167
MS.gene71443.t1 MTR_7g098040 69.231 104 32 0 8 111 14 117 5.74e-50 167
MS.gene71443.t1 MTR_7g098200 68.269 104 33 0 8 111 9 112 3.83e-49 165
MS.gene71443.t1 MTR_7g098200 68.269 104 33 0 8 111 9 112 3.84e-49 164
MS.gene71443.t1 MTR_3g072300 60.577 104 41 0 8 111 4 107 1.04e-43 150
MS.gene71443.t1 MTR_1g042310 80.556 72 14 0 40 111 1 72 4.77e-36 129
MS.gene71443.t1 MTR_3g462710 53.982 113 46 2 1 113 1 107 3.30e-35 127
MS.gene71443.t1 MTR_1g041885 81.818 66 12 0 47 112 1 66 1.07e-33 122
MS.gene71443.t1 MTR_1g042310 83.077 65 11 0 47 111 1 65 5.27e-33 120
MS.gene71443.t1 MTR_7g098230 70.423 71 21 0 41 111 39 109 1.14e-31 117
MS.gene71443.t1 MTR_3g005010 53.211 109 49 1 3 111 7 113 1.25e-31 117
MS.gene71443.t1 MTR_7g067010 47.115 104 55 0 8 111 4 107 1.25e-30 114
MS.gene71443.t1 MTR_4g015080 51.429 105 46 2 7 108 10 112 2.15e-30 113
MS.gene71443.t1 MTR_7g098220 65.217 69 24 0 43 111 1 69 5.28e-29 109
MS.gene71443.t1 MTR_4g033395 47.959 98 51 0 14 111 18 115 1.76e-27 105
MS.gene71443.t1 MTR_4g033375 46.939 98 52 0 14 111 18 115 9.74e-27 103
MS.gene71443.t1 MTR_3g069500 45.192 104 57 0 8 111 15 118 3.16e-26 102
MS.gene71443.t1 MTR_3g069420 41.905 105 61 0 8 112 14 118 4.28e-26 101
MS.gene71443.t1 MTR_0294s0020 48.485 99 51 0 9 107 12 110 1.21e-25 100
MS.gene71443.t1 MTR_4g033330 48.936 94 48 0 14 107 17 110 5.33e-25 98.2
MS.gene71443.t1 MTR_4g033325 46.809 94 50 0 14 107 17 110 7.58e-25 97.8
MS.gene71443.t1 MTR_4g033310 48.936 94 48 0 14 107 17 110 7.73e-25 97.8
MS.gene71443.t1 MTR_4g033315 48.936 94 48 0 14 107 17 110 1.18e-24 97.4
MS.gene71443.t1 MTR_4g033310 48.936 94 48 0 14 107 17 110 1.18e-24 97.4
MS.gene71443.t1 MTR_7g065080 39.474 114 66 1 1 111 1 114 1.40e-24 97.4
MS.gene71443.t1 MTR_3g069440 45.745 94 51 0 14 107 19 112 1.43e-22 91.7
MS.gene71443.t1 MTR_4g019580 38.235 102 63 0 10 111 18 119 2.09e-22 91.3
MS.gene71443.t1 MTR_8g038880 50.588 85 42 0 27 111 19 103 4.77e-22 90.1
MS.gene71443.t1 MTR_5g014150 34.951 103 67 0 9 111 25 127 9.76e-22 89.4
MS.gene71443.t1 MTR_3g112460 38.144 97 60 0 14 110 9 105 1.64e-21 88.6
MS.gene71443.t1 MTR_7g006540 42.202 109 61 2 11 117 44 152 2.22e-20 85.5
MS.gene71443.t1 MTR_3g014790 41.414 99 58 0 13 111 2 100 3.02e-20 85.1
MS.gene71443.t1 MTR_3g112470 39.175 97 59 0 14 110 9 105 3.58e-20 84.7
MS.gene71443.t1 MTR_4g024930 33.333 99 66 0 12 110 20 118 4.31e-20 84.3
MS.gene71443.t1 MTR_4g098800 35.577 104 67 0 9 112 17 120 4.65e-20 84.3
MS.gene71443.t1 MTR_4g098800 35.577 104 67 0 9 112 82 185 5.25e-20 84.3
MS.gene71443.t1 MTR_7g010820 35.922 103 66 0 9 111 7 109 1.39e-19 83.2
MS.gene71443.t1 MTR_5g012290 43.182 88 50 0 18 105 17 104 8.48e-19 80.9
MS.gene71443.t1 MTR_7g088825 37.500 104 65 0 7 110 1 104 1.01e-18 80.5
MS.gene71443.t1 MTR_4g101380 42.045 88 51 0 18 105 17 104 1.19e-18 80.5
MS.gene71443.t1 MTR_3g112450 38.298 94 58 0 17 110 12 105 1.33e-18 80.1
MS.gene71443.t1 MTR_4g107670 39.450 109 62 1 1 109 1 105 4.04e-18 79.0
MS.gene71443.t1 MTR_5g012270 37.374 99 56 1 2 100 9 101 4.50e-18 79.0
MS.gene71443.t1 MTR_7g088820 39.450 109 59 2 2 110 6 107 1.43e-17 77.4
MS.gene71443.t1 MTR_5g038060 41.860 86 50 0 26 111 29 114 1.49e-17 77.4
MS.gene71443.t1 MTR_8g469480 41.975 81 47 0 31 111 41 121 3.34e-17 76.3
MS.gene71443.t1 MTR_7g088790 40.426 94 56 0 17 110 18 111 6.60e-17 75.5
MS.gene71443.t1 MTR_5g055000 41.176 85 50 0 26 110 16 100 7.52e-17 75.1
MS.gene71443.t1 MTR_7g013270 35.227 88 57 0 18 105 80 167 1.22e-16 74.7
MS.gene71443.t1 MTR_2g005130 37.500 104 65 0 9 112 21 124 1.38e-16 74.7
MS.gene71443.t1 MTR_5g038380 39.796 98 59 0 14 111 14 111 7.73e-16 72.4
MS.gene71443.t1 MTR_1g116930 33.010 103 68 1 9 111 5 106 9.58e-16 72.0
MS.gene71443.t1 MTR_7g498330 65.909 44 14 1 8 51 9 51 2.64e-15 65.1
MS.gene71443.t1 MTR_1g026750 33.333 93 60 1 14 104 2 94 4.64e-15 70.1
MS.gene71443.t1 MTR_7g098080 57.143 49 18 1 33 78 13 61 7.28e-15 64.3
MS.gene71443.t1 MTR_8g469470 29.808 104 73 0 8 111 12 115 8.03e-15 69.3
MS.gene71443.t1 MTR_7g005930 33.333 102 66 1 10 111 19 118 9.65e-15 69.3
MS.gene71443.t1 MTR_4g114340 39.216 102 62 0 8 109 4 105 1.54e-14 68.6
MS.gene71443.t1 MTR_7g098210 47.692 65 19 1 47 111 1 50 2.98e-14 67.8
MS.gene71443.t1 MTR_1g026740 36.585 82 52 0 30 111 19 100 4.25e-14 67.4
MS.gene71443.t1 MTR_5g038190 35.106 94 61 0 19 112 15 108 1.67e-13 65.9
MS.gene71443.t1 MTR_8g469310 37.895 95 59 0 15 109 13 107 2.74e-13 65.1
MS.gene71443.t1 MTR_1g026770 39.726 73 44 0 30 102 17 89 2.76e-13 65.1
MS.gene71443.t1 MTR_5g085850 38.384 99 60 1 12 109 13 111 1.56e-12 62.8
MS.gene71443.t1 MTR_7g064160 26.050 119 87 1 1 118 1 119 3.00e-12 58.9
MS.gene71443.t1 MTR_1g009200 31.683 101 65 1 8 108 11 107 1.08e-11 60.5
MS.gene71443.t1 MTR_8g087810 34.000 100 66 0 9 108 2 101 1.82e-11 60.1
MS.gene71443.t1 MTR_7g064780 26.087 115 79 2 8 116 6 120 7.16e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71443.t1 AT5G46040 75.238 105 26 0 7 111 6 110 2.64e-52 173
MS.gene71443.t1 AT5G46050 72.642 106 29 0 7 112 6 111 2.39e-49 165
MS.gene71443.t1 AT2G40460 58.559 111 42 1 1 111 1 107 9.02e-44 150
MS.gene71443.t1 AT3G54140 51.754 114 49 2 1 114 1 108 1.97e-36 130
MS.gene71443.t1 AT3G54140 51.754 114 49 2 1 114 1 108 1.97e-36 130
MS.gene71443.t1 AT2G02040 53.097 113 51 1 1 111 12 124 5.71e-36 129
MS.gene71443.t1 AT5G01180 49.533 107 52 1 8 114 5 109 1.42e-32 119
MS.gene71443.t1 AT5G01180 49.533 107 52 1 8 114 5 109 1.42e-32 119
MS.gene71443.t1 AT5G01180 49.533 107 52 1 8 114 5 109 1.42e-32 119
MS.gene71443.t1 AT2G02020 45.133 113 59 1 3 112 14 126 8.66e-32 117
MS.gene71443.t1 AT2G02020 45.133 113 59 1 3 112 14 126 9.24e-32 117
MS.gene71443.t1 AT1G62200 43.220 118 55 2 1 108 15 130 2.04e-28 108
MS.gene71443.t1 AT1G62200 43.220 118 55 2 1 108 15 130 2.07e-28 108
MS.gene71443.t1 AT1G62200 43.220 118 55 2 1 108 29 144 2.23e-28 108
MS.gene71443.t1 AT3G54450 55.814 86 37 1 26 111 11 95 9.68e-26 100
MS.gene71443.t1 AT1G22570 48.113 106 54 1 2 107 5 109 1.08e-25 100
MS.gene71443.t1 AT1G22550 46.226 106 56 1 2 107 5 109 7.28e-25 98.2
MS.gene71443.t1 AT1G22540 52.083 96 46 0 16 111 18 113 8.67e-25 97.8
MS.gene71443.t1 AT3G53960 41.346 104 61 0 8 111 17 120 5.39e-24 95.9
MS.gene71443.t1 AT2G37900 38.462 104 64 0 8 111 18 121 9.28e-24 95.1
MS.gene71443.t1 AT1G72120 48.571 105 53 1 7 111 9 112 9.49e-23 92.0
MS.gene71443.t1 AT1G72140 40.909 110 64 1 2 111 9 117 1.00e-22 92.0
MS.gene71443.t1 AT1G72125 50.476 105 51 1 7 111 9 112 6.70e-22 89.7
MS.gene71443.t1 AT1G32450 37.864 103 64 0 9 111 18 120 1.70e-21 88.6
MS.gene71443.t1 AT4G21680 37.931 116 66 2 9 118 9 124 6.44e-21 87.0
MS.gene71443.t1 AT1G12110 42.391 92 53 0 18 109 17 108 1.40e-20 85.9
MS.gene71443.t1 AT5G13400 43.750 96 53 1 17 111 52 147 1.46e-20 85.9
MS.gene71443.t1 AT1G59740 38.596 114 67 1 2 112 12 125 3.24e-19 82.0
MS.gene71443.t1 AT1G72130 43.182 88 50 0 24 111 21 108 3.53e-19 82.0
MS.gene71443.t1 AT1G68570 39.091 110 62 3 1 110 1 105 4.05e-18 79.0
MS.gene71443.t1 AT1G69870 40.230 87 52 0 25 111 48 134 5.03e-17 75.9
MS.gene71443.t1 AT1G22570 56.061 66 29 0 42 107 7 72 6.58e-17 75.5
MS.gene71443.t1 AT5G62730 37.500 96 60 0 14 109 42 137 1.20e-15 72.0
MS.gene71443.t1 AT1G68570 46.377 69 37 0 42 110 7 75 1.56e-15 71.6
MS.gene71443.t1 AT5G19640 36.145 83 53 0 29 111 60 142 4.63e-15 70.1
MS.gene71443.t1 AT2G26690 39.216 102 62 0 8 109 4 105 9.34e-15 69.3
MS.gene71443.t1 AT1G33440 36.937 111 64 1 9 113 19 129 3.13e-14 67.8
MS.gene71443.t1 AT5G28470 36.559 93 56 1 24 113 13 105 3.32e-14 67.8
MS.gene71443.t1 AT5G28470 36.559 93 56 1 24 113 13 105 3.42e-14 67.8
MS.gene71443.t1 AT3G25260 35.644 101 61 1 5 105 2 98 1.14e-13 66.2
MS.gene71443.t1 AT3G16180 36.364 88 55 1 22 108 16 103 2.18e-13 65.5
MS.gene71443.t1 AT1G27080 37.349 83 52 0 26 108 12 94 3.77e-13 64.7
MS.gene71443.t1 AT1G69850 34.615 104 67 1 7 109 3 106 3.97e-13 64.7
MS.gene71443.t1 AT1G52190 34.940 83 54 0 26 108 19 101 2.20e-12 62.8
MS.gene71443.t1 AT3G01350 35.227 88 56 1 25 111 3 90 1.31e-11 60.5
MS.gene71443.t1 AT1G69860 29.268 82 58 0 26 107 16 97 2.01e-11 59.7
MS.gene71443.t1 AT5G14940 33.333 84 56 0 28 111 7 90 4.53e-11 58.9
MS.gene71443.t1 AT5G11570 33.333 87 55 1 14 100 2 85 9.12e-11 57.8

Find 30 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAAGAATGGCATATTA+TGG 0.240890 1.3:-53935403 MS.gene71443:CDS
ATTTGGATGACACCTATATT+AGG 0.308097 1.3:-53935289 MS.gene71443:CDS
AGCTTTCCAACCACCAGTTT+TGG 0.319121 1.3:+53936538 None:intergenic
CCAATTGGGTTGGGACCATT+TGG 0.334713 1.3:-53935306 MS.gene71443:CDS
GCAACATAAGCACCTAATAT+AGG 0.383360 1.3:+53935277 None:intergenic
CAAGTAACAATGTCACCAAT+TGG 0.391039 1.3:-53935321 MS.gene71443:CDS
TGCATCCTTCATTTATCTCT+CGG 0.404223 1.3:-53935218 MS.gene71443:CDS
CCTGTTCTTAGATCCAAAAC+TGG 0.409508 1.3:-53936551 MS.gene71443:CDS
TTGAAGGATAACTATGGAAG+AGG 0.437888 1.3:-53936630 None:intergenic
GAAGCTTCTTTGTGAGGAAA+AGG 0.440309 1.3:+53935362 None:intergenic
AAAGCTTGTTCCTTTGTTGT+TGG 0.460711 1.3:-53936521 MS.gene71443:intron
TACTTGCAGTCACTGTTCCT+TGG 0.461462 1.3:+53935338 None:intergenic
CCAAATGGTCCCAACCCAAT+TGG 0.463312 1.3:+53935306 None:intergenic
GTATGAAGTATTTGAAAGAA+TGG 0.490979 1.3:-53935413 MS.gene71443:CDS
AAGGAGGAGTACACAGAAGA+TGG 0.498352 1.3:-53936596 MS.gene71443:CDS
AGAGGGCAGAGTAGTGAAGG+AGG 0.501220 1.3:-53936612 MS.gene71443:CDS
TAATATAGGTGTCATCCAAA+TGG 0.502308 1.3:+53935291 None:intergenic
AAGTAACAATGTCACCAATT+GGG 0.513695 1.3:-53935320 MS.gene71443:CDS
TTAGATCCAAAACTGGTGGT+TGG 0.517084 1.3:-53936544 MS.gene71443:CDS
AACAATGTCACCAATTGGGT+TGG 0.525729 1.3:-53935316 MS.gene71443:CDS
CTTGGTGAAGCTTCTTTGTG+AGG 0.532765 1.3:+53935356 None:intergenic
ACAATGTCACCAATTGGGTT+GGG 0.534361 1.3:-53935315 MS.gene71443:CDS
TATGTTGCTGATGCTCACCT+TGG 0.550523 1.3:-53935262 MS.gene71443:CDS
GGAAGAGGGCAGAGTAGTGA+AGG 0.558901 1.3:-53936615 MS.gene71443:CDS
ATTTATCTCTCGGTAAGCAA+TGG 0.576365 1.3:-53935208 MS.gene71443:CDS
GCTTACCGAGAGATAAATGA+AGG 0.578740 1.3:+53935213 None:intergenic
GATGGAACTGTGAATCTCAA+AGG 0.591833 1.3:-53936578 MS.gene71443:CDS
TGAAGGATAACTATGGAAGA+GGG 0.606507 1.3:-53936629 None:intergenic
GTTCTTAGATCCAAAACTGG+TGG 0.657227 1.3:-53936548 MS.gene71443:CDS
CTCACAAAGAAGCTTCACCA+AGG 0.670831 1.3:-53935355 MS.gene71443:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAATTGATCATAAAT+TGG - chr1.3:53935874-53935893 MS.gene71443:intron 15.0%
!! TTTCTACATTTATAAATTGA+AGG + chr1.3:53936334-53936353 None:intergenic 15.0%
!! TTTCTTATAAGAAAAACAAT+AGG - chr1.3:53936275-53936294 MS.gene71443:intron 15.0%
!!! TGAGAAAACTTATTTTTTAA+GGG - chr1.3:53935650-53935669 MS.gene71443:intron 15.0%
!! AACACATATCAAAACAAAAA+TGG - chr1.3:53935960-53935979 MS.gene71443:intron 20.0%
!! TAAATCCTAACAAAAAGATA+TGG + chr1.3:53935328-53935347 None:intergenic 20.0%
!! TAGCTAATAATAACAATTCA+TGG + chr1.3:53935375-53935394 None:intergenic 20.0%
!! TCTTATAAGAAAATTGTGAT+TGG + chr1.3:53936267-53936286 None:intergenic 20.0%
!! TTATTCTGTAAAAGATTGAA+GGG - chr1.3:53935475-53935494 MS.gene71443:intron 20.0%
!!! AAGAAGAAAAGTATTATAGA+AGG - chr1.3:53935719-53935738 MS.gene71443:intron 20.0%
!!! ATAGAACTTTAGAGTAAAAA+GGG - chr1.3:53935452-53935471 MS.gene71443:intron 20.0%
!!! GTGAGAAAACTTATTTTTTA+AGG - chr1.3:53935649-53935668 MS.gene71443:intron 20.0%
!!! TATAGAACTTTAGAGTAAAA+AGG - chr1.3:53935451-53935470 MS.gene71443:intron 20.0%
!!! TATAGTTGTAGATAACTTTT+TGG - chr1.3:53935681-53935700 MS.gene71443:intron 20.0%
! ATACTTCAACATGTATTCAT+TGG - chr1.3:53935510-53935529 MS.gene71443:intron 25.0%
! GATAGAAAATAAAATGTGCA+GGG + chr1.3:53935815-53935834 None:intergenic 25.0%
! GTTATTCTGTAAAAGATTGA+AGG - chr1.3:53935474-53935493 MS.gene71443:intron 25.0%
! TGATAGAAAATAAAATGTGC+AGG + chr1.3:53935816-53935835 None:intergenic 25.0%
! TTCTATCATATTTGATCCAA+TGG - chr1.3:53935828-53935847 MS.gene71443:intron 25.0%
! TTTGAAAGAATGGCATATTA+TGG - chr1.3:53936412-53936431 MS.gene71443:intron 25.0%
!! AATCTCCATATCTTTTTGTT+AGG - chr1.3:53935320-53935339 MS.gene71443:CDS 25.0%
!! ACATCTTTATGACCATTTTA+TGG + chr1.3:53935934-53935953 None:intergenic 25.0%
!! GTATGAAGTATTTGAAAGAA+TGG - chr1.3:53936402-53936421 MS.gene71443:intron 25.0%
!!! ACTAATTCTGAAATTCAAAG+AGG - chr1.3:53935537-53935556 MS.gene71443:intron 25.0%
!!! ATTGAGCATTGAAATGTTTT+TGG - chr1.3:53936361-53936380 MS.gene71443:intron 25.0%
!!! CTAATTCTGAAATTCAAAGA+GGG - chr1.3:53935538-53935557 MS.gene71443:intron 25.0%
!!! GACTGTTTTTTTTTAAGGAA+AGG + chr1.3:53935787-53935806 None:intergenic 25.0%
!!! TAAACTTATTTTGTTGCGTT+TGG + chr1.3:53936032-53936051 None:intergenic 25.0%
!!! TGGAAGACTGTTTTTTTTTA+AGG + chr1.3:53935792-53935811 None:intergenic 25.0%
AAGTAACAATGTCACCAATT+GGG - chr1.3:53936495-53936514 MS.gene71443:intron 30.0%
AGACAAGAAAATCTTACCAT+TGG + chr1.3:53935847-53935866 None:intergenic 30.0%
ATTTGGATGACACCTATATT+AGG - chr1.3:53936526-53936545 MS.gene71443:CDS 30.0%
CATGGTGTAACAATTATTCA+TGG + chr1.3:53935357-53935376 None:intergenic 30.0%
TTCCTGAATGAAACAATTCT+TGG - chr1.3:53936130-53936149 MS.gene71443:intron 30.0%
! GGTAAGATTTTCTTGTCTAA+TGG - chr1.3:53935849-53935868 MS.gene71443:intron 30.0%
! TAATATAGGTGTCATCCAAA+TGG + chr1.3:53936527-53936546 None:intergenic 30.0%
AGAAAATAAAATGTGCAGGG+TGG + chr1.3:53935812-53935831 None:intergenic 35.0%
ATTTATCTCTCGGTAAGCAA+TGG - chr1.3:53936607-53936626 MS.gene71443:CDS 35.0%
CAAAAGAGAGAAATGAGAGA+AGG - chr1.3:53935579-53935598 MS.gene71443:intron 35.0%
CAAGTAACAATGTCACCAAT+TGG - chr1.3:53936494-53936513 MS.gene71443:intron 35.0%
CACACTCGTTTACCATAAAA+TGG - chr1.3:53935919-53935938 MS.gene71443:intron 35.0%
GCAACATAAGCACCTAATAT+AGG + chr1.3:53936541-53936560 None:intergenic 35.0%
TGCATCCTTCATTTATCTCT+CGG - chr1.3:53936597-53936616 MS.gene71443:CDS 35.0%
TTCACCAATGACGAATTGTT+CGG - chr1.3:53936091-53936110 MS.gene71443:intron 35.0%
! AAAGCTTGTTCCTTTGTTGT+TGG - chr1.3:53935294-53935313 MS.gene71443:CDS 35.0%
! AGATTGAAGGGTGATAATGA+TGG - chr1.3:53935487-53935506 MS.gene71443:intron 35.0%
! CTTCTCTCATTTCTCTCTTT+TGG + chr1.3:53935581-53935600 None:intergenic 35.0%
! GGAGATTTTACCAACAACAA+AGG + chr1.3:53935307-53935326 None:intergenic 35.0%
AACAATGTCACCAATTGGGT+TGG - chr1.3:53936499-53936518 MS.gene71443:intron 40.0%
ACAATGTCACCAATTGGGTT+GGG - chr1.3:53936500-53936519 MS.gene71443:intron 40.0%
ATATTGCTCTCCTACAAGTG+TGG - chr1.3:53935607-53935626 MS.gene71443:intron 40.0%
CCTGTTCTTAGATCCAAAAC+TGG - chr1.3:53935264-53935283 MS.gene71443:CDS 40.0%
GAAAAGTCCAAAAACGACCA+AGG + chr1.3:53936573-53936592 None:intergenic 40.0%
GAAGCTTCTTTGTGAGGAAA+AGG + chr1.3:53936456-53936475 None:intergenic 40.0%
GATGGAACTGTGAATCTCAA+AGG - chr1.3:53935237-53935256 MS.gene71443:CDS 40.0%
GCTTACCGAGAGATAAATGA+AGG + chr1.3:53936605-53936624 None:intergenic 40.0%
GGCCAAGAATTGTTTCATTC+AGG + chr1.3:53936135-53936154 None:intergenic 40.0%
GTTCTTAGATCCAAAACTGG+TGG - chr1.3:53935267-53935286 MS.gene71443:CDS 40.0%
TCTTCCGAACAATTCGTCAT+TGG + chr1.3:53936098-53936117 None:intergenic 40.0%
TTAGATCCAAAACTGGTGGT+TGG - chr1.3:53935271-53935290 MS.gene71443:CDS 40.0%
TTATGGTAAACGAGTGTGAC+AGG + chr1.3:53935917-53935936 None:intergenic 40.0%
! CTCTCATTTTCCACACTTGT+AGG + chr1.3:53935620-53935639 None:intergenic 40.0%
!! TACATAGCGCTGGTTGAAAA+TGG + chr1.3:53936203-53936222 None:intergenic 40.0%
!!! CCAGTTTTGGATCTAAGAAC+AGG + chr1.3:53935267-53935286 None:intergenic 40.0%
ACCAAGCAATTGAGCTCAGA+TGG - chr1.3:53936059-53936078 MS.gene71443:intron 45.0%
ACCATCTGAGCTCAATTGCT+TGG + chr1.3:53936063-53936082 None:intergenic 45.0%
ACGAATTGTTCGGAAGAACC+TGG - chr1.3:53936101-53936120 MS.gene71443:intron 45.0%
ATGGTAATCCAGAGTGAGTG+CGG + chr1.3:53936178-53936197 None:intergenic 45.0%
CGAATTGTTCGGAAGAACCT+GGG - chr1.3:53936102-53936121 MS.gene71443:intron 45.0%
CTCACAAAGAAGCTTCACCA+AGG - chr1.3:53936460-53936479 MS.gene71443:intron 45.0%
CTTGGTGAAGCTTCTTTGTG+AGG + chr1.3:53936462-53936481 None:intergenic 45.0%
GTCGTGAGATAAGGATAGTC+TGG + chr1.3:53936156-53936175 None:intergenic 45.0%
TACTTGCAGTCACTGTTCCT+TGG + chr1.3:53936480-53936499 None:intergenic 45.0%
TATGTTGCTGATGCTCACCT+TGG - chr1.3:53936553-53936572 MS.gene71443:CDS 45.0%
TCAACCAGCGCTATGTAGAA+AGG - chr1.3:53936206-53936225 MS.gene71443:intron 45.0%
TTCATTCAGGAATCGAACCC+AGG + chr1.3:53936122-53936141 None:intergenic 45.0%
TTGACCTTTCTACATAGCGC+TGG + chr1.3:53936213-53936232 None:intergenic 45.0%
! AAGGAGGAGTACACAGAAGA+TGG - chr1.3:53935219-53935238 MS.gene71443:CDS 45.0%
! AGCTTTCCAACCACCAGTTT+TGG + chr1.3:53935280-53935299 None:intergenic 45.0%
!! ATGCTCACCTTGGTCGTTTT+TGG - chr1.3:53936563-53936582 MS.gene71443:CDS 45.0%
CCAAATGGTCCCAACCCAAT+TGG + chr1.3:53936512-53936531 None:intergenic 50.0%
!! CCAATTGGGTTGGGACCATT+TGG - chr1.3:53936509-53936528 MS.gene71443:intron 50.0%
!! GCGCTGGTTGAAAATGGTCA+TGG + chr1.3:53936197-53936216 None:intergenic 50.0%
AGAGGGCAGAGTAGTGAAGG+AGG - chr1.3:53935203-53935222 MS.gene71443:CDS 55.0%
GGAAGAGGGCAGAGTAGTGA+AGG - chr1.3:53935200-53935219 MS.gene71443:CDS 55.0%
GTGAGTGCGGTCGTGAGATA+AGG + chr1.3:53936165-53936184 None:intergenic 55.0%
TCTCACGACCGCACTCACTC+TGG - chr1.3:53936167-53936186 MS.gene71443:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 53935198 53936639 53935198 ID=MS.gene71443
chr1.3 mRNA 53935198 53936639 53935198 ID=MS.gene71443.t1;Parent=MS.gene71443
chr1.3 exon 53936522 53936639 53936522 ID=MS.gene71443.t1.exon1;Parent=MS.gene71443.t1
chr1.3 CDS 53936522 53936639 53936522 ID=cds.MS.gene71443.t1;Parent=MS.gene71443.t1
chr1.3 exon 53935198 53935436 53935198 ID=MS.gene71443.t1.exon2;Parent=MS.gene71443.t1
chr1.3 CDS 53935198 53935436 53935198 ID=cds.MS.gene71443.t1;Parent=MS.gene71443.t1
Gene Sequence

>MS.gene71443

ATGGAAGAGGGCAGAGTAGTGAAGGAGGAGTACACAGAAGATGGAACTGTGAATCTCAAAGGAAAACCTGTTCTTAGATCCAAAACTGGTGGTTGGAAAGCTTGTTCCTTTGTTGTTGGTAAAATCTCCATATCTTTTTGTTAGGATTTAGTTACACCATGAATAATTGTTACACCATGAATTGTTATTATTAGCTAAAAGTGTAAAACTTTTAACATTTTTTTTATATAGTCATTTTCATCTGATTTATATATATAGAACTTTAGAGTAAAAAGGGTTATTCTGTAAAAGATTGAAGGGTGATAATGATGGATACTTCAACATGTATTCATTGGTCTTACTAATTCTGAAATTCAAAGAGGGAGAGAGATAAGCAAATACCAAAAGAGAGAAATGAGAGAAGGTCAAAATATTGCTCTCCTACAAGTGTGGAAAATGAGAGAAAACATGAGTGAGAAAACTTATTTTTTAAGGGATTAAAAATATAGTTGTAGATAACTTTTTGGCGTAAAACATTTATAAAGAAGAAAAGTATTATAGAAGGAAAAATGTTTTGTCTAACTTGTACAAGTAAATAAGTCTTGCACCTTTCCTTAAAAAAAAACAGTCTTCCACCCTGCACATTTTATTTTCTATCATATTTGATCCAATGGTAAGATTTTCTTGTCTAATGGTGAAAACAAATTGATCATAAATTGGTCATACATGATGTATTACCTGTCACACTCGTTTACCATAAAATGGTCATAAAGATGTGTCTTTAACACATATCAAAACAAAAATGGTACAAGACATAGTCAAACTCAACTACAATAAAAGATACATGATAAACCAAACGCAACAAAATAAGTTTAAATGTTAACCAAGCAATTGAGCTCAGATGGTAAATAAGTTTCACCAATGACGAATTGTTCGGAAGAACCTGGGTTCGATTCCTGAATGAAACAATTCTTGGCCAGACTATCCTTATCTCACGACCGCACTCACTCTGGATTACCATGACCATTTTCAACCAGCGCTATGTAGAAAGGTCAATAACTCTATTTCTACTACTAACAAATCATCTCCAATCACAATTTTCTTATAAGAAAAACAATAGGTTCATGTCTACATCAACACTTCAAAAGCTTCAACCTTCAATTTATAAATGTAGAAACTTTATTATTGAGCATTGAAATGTTTTTGGTTGAATGTATGTTGCAGTGTATGAAGTATTTGAAAGAATGGCATATTATGGAATATCATCGAATTTGATCCTTTTCCTCACAAAGAAGCTTCACCAAGGAACAGTGACTGCAAGTAACAATGTCACCAATTGGGTTGGGACCATTTGGATGACACCTATATTAGGTGCTTATGTTGCTGATGCTCACCTTGGTCGTTTTTGGACTTTTCTCATTGCATCCTTCATTTATCTCTCGGTAAGCAATGGTATGCTATAA
Protein sequence

>MS.gene71443.t1

MEEGRVVKEEYTEDGTVNLKGKPVLRSKTGGWKACSFVVVYEVFERMAYYGISSNLILFLTKKLHQGTVTASNNVTNWVGTIWMTPILGAYVADAHLGRFWTFLIASFIYLSVSNGML