AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059161


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059161.t1 MTR_1g007905 91.005 189 16 1 1 189 1 188 4.52e-123 346
MS.gene059161.t1 MTR_3g091680 55.844 154 53 5 37 189 1 140 6.26e-44 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059161.t1 AT5G53030 38.333 180 70 9 45 189 40 213 1.70e-19 82.8
MS.gene059161.t1 AT5G53030 38.333 180 70 9 45 189 40 213 1.94e-19 83.2
MS.gene059161.t1 AT4G27810 35.176 199 78 11 9 188 1 167 5.51e-11 59.3

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGGGCAGTGTGAATTT+TGG 0.173203 1.2:-12076496 MS.gene059161:CDS
TCCTCCTAGGTTGTTGTCTT+TGG 0.204117 1.2:-12076626 MS.gene059161:CDS
TTGTTACCTTCTCTTTCTTT+TGG 0.308850 1.2:+12076670 None:intergenic
ACCATCACCAACAACTGTTT+TGG 0.363972 1.2:-12076575 MS.gene059161:CDS
TAACAACGTTGTTAGAGCTT+TGG 0.381783 1.2:-12076653 MS.gene059161:CDS
CACATGGTACTTCACATTTG+TGG 0.395630 1.2:-12076342 MS.gene059161:CDS
TTGGGTCTAGTAGGTGGGGT+GGG 0.401963 1.2:-12076477 MS.gene059161:CDS
ATCATGATGATGATGAATTA+AGG 0.404054 1.2:-12076858 MS.gene059161:CDS
ACATGGTACTTCACATTTGT+GGG 0.406088 1.2:-12076341 None:intergenic
ATAGCCGTCGTTCGCGGTGG+TGG 0.414893 1.2:+12076757 None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG 0.452572 1.2:-12076411 MS.gene059161:CDS
TTTAAGTGGGAGGAAGCACC+AGG 0.462547 1.2:-12076724 MS.gene059161:CDS
GTCGTTCGCGGTGGTGGAGT+TGG 0.473237 1.2:+12076763 None:intergenic
TAGGTTGTTGTCTTTGGAGA+GGG 0.474181 1.2:-12076620 MS.gene059161:CDS
GGACCTTCCAAAACAGTTGT+TGG 0.481823 1.2:+12076568 None:intergenic
TGACATGACCTAGGCTTCCC+TGG 0.485647 1.2:+12076706 None:intergenic
GGTGCTTCCTCCCACTTAAA+CGG 0.493829 1.2:+12076727 None:intergenic
GAGCTTTGGAACTTCCTCCT+AGG 0.498331 1.2:-12076639 MS.gene059161:CDS
TTTGGGTCTAGTAGGTGGGG+TGG 0.500229 1.2:-12076478 MS.gene059161:CDS
GACACTGCACGTCCAACGTA+TGG 0.515972 1.2:+12076547 None:intergenic
GATCACAAGAGTTAAGAGAA+GGG 0.526664 1.2:-12076374 MS.gene059161:CDS
CTAGGTTGTTGTCTTTGGAG+AGG 0.533267 1.2:-12076621 MS.gene059161:CDS
TTCAAGTTGGAGTGTTGATG+GGG 0.539072 1.2:-12076410 MS.gene059161:CDS
CTATATCGATACCGTTTAAG+TGG 0.539738 1.2:-12076738 MS.gene059161:CDS
AACTCCACCACCGCGAACGA+CGG 0.539846 1.2:-12076761 MS.gene059161:CDS
AGGAAGCACCAGGGAAGCCT+AGG 0.540552 1.2:-12076714 MS.gene059161:CDS
ATCGATATAGCCGTCGTTCG+CGG 0.559277 1.2:+12076751 None:intergenic
AGATCACAAGAGTTAAGAGA+AGG 0.565850 1.2:-12076375 MS.gene059161:CDS
ATCACAAGAGTTAAGAGAAG+GGG 0.589130 1.2:-12076373 MS.gene059161:CDS
TCCAAAACAGTTGTTGGTGA+TGG 0.590521 1.2:+12076574 None:intergenic
ATCGATACCGTTTAAGTGGG+AGG 0.600972 1.2:-12076734 MS.gene059161:CDS
CCAGAGCCAAAAGAAAGAGA+AGG 0.615358 1.2:-12076676 MS.gene059161:CDS
GTATAGGGATAGTAATAACA+AGG 0.626999 1.2:-12076512 MS.gene059161:CDS
CACGGTCGTGATAACACTGG+TGG 0.632454 1.2:+12076802 None:intergenic
TCCAAAGACAACAACCTAGG+AGG 0.639929 1.2:+12076625 None:intergenic
GGTCGTGTATGACATGACCT+AGG 0.639981 1.2:+12076697 None:intergenic
TATATCGATACCGTTTAAGT+GGG 0.641926 1.2:-12076737 MS.gene059161:CDS
TCAAGTTGGAGTGTTGATGG+GGG 0.642096 1.2:-12076409 MS.gene059161:CDS
AAAAGTCAAAACTACCCTCA+CGG 0.642581 1.2:+12076431 None:intergenic
AATAATAGAATTGACCGTGA+GGG 0.656653 1.2:-12076445 MS.gene059161:CDS
CTCTCCAAAGACAACAACCT+AGG 0.661580 1.2:+12076622 None:intergenic
AAACACGGTCGTGATAACAC+TGG 0.682213 1.2:+12076799 None:intergenic
AAATAATAGAATTGACCGTG+AGG 0.684752 1.2:-12076446 MS.gene059161:CDS
GATATAGCCGTCGTTCGCGG+TGG 0.697862 1.2:+12076754 None:intergenic
TATAGGGATAGTAATAACAA+GGG 0.698970 1.2:-12076511 MS.gene059161:CDS
AGAAGGGGAAGCTTCTCACA+TGG 0.717228 1.2:-12076358 MS.gene059161:CDS
TTAAGTGGGAGGAAGCACCA+GGG 0.769568 1.2:-12076723 MS.gene059161:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATAGGGATAGTAATAACAA+GGG - chr1.2:12076717-12076736 MS.gene059161:CDS 25.0%
!! ATCATGATGATGATGAATTA+AGG - chr1.2:12076370-12076389 MS.gene059161:CDS 25.0%
AAATAATAGAATTGACCGTG+AGG - chr1.2:12076782-12076801 MS.gene059161:CDS 30.0%
AATAATAGAATTGACCGTGA+GGG - chr1.2:12076783-12076802 MS.gene059161:CDS 30.0%
GTATAGGGATAGTAATAACA+AGG - chr1.2:12076716-12076735 MS.gene059161:CDS 30.0%
TATATCGATACCGTTTAAGT+GGG - chr1.2:12076491-12076510 MS.gene059161:CDS 30.0%
! TTGTTACCTTCTCTTTCTTT+TGG + chr1.2:12076561-12076580 None:intergenic 30.0%
!!! GTAGTTTTGACTTTTCAAGT+TGG - chr1.2:12076805-12076824 MS.gene059161:CDS 30.0%
AAAAGTCAAAACTACCCTCA+CGG + chr1.2:12076800-12076819 None:intergenic 35.0%
AGATCACAAGAGTTAAGAGA+AGG - chr1.2:12076853-12076872 MS.gene059161:CDS 35.0%
ATCACAAGAGTTAAGAGAAG+GGG - chr1.2:12076855-12076874 MS.gene059161:CDS 35.0%
CTATATCGATACCGTTTAAG+TGG - chr1.2:12076490-12076509 MS.gene059161:CDS 35.0%
GATCACAAGAGTTAAGAGAA+GGG - chr1.2:12076854-12076873 MS.gene059161:CDS 35.0%
TAACAACGTTGTTAGAGCTT+TGG - chr1.2:12076575-12076594 MS.gene059161:CDS 35.0%
!! TTTCAAGTTGGAGTGTTGAT+GGG - chr1.2:12076817-12076836 MS.gene059161:CDS 35.0%
!!! TTTTCAAGTTGGAGTGTTGA+TGG - chr1.2:12076816-12076835 MS.gene059161:CDS 35.0%
CACATGGTACTTCACATTTG+TGG - chr1.2:12076886-12076905 MS.gene059161:CDS 40.0%
! AACAAGGGCAGTGTGAATTT+TGG - chr1.2:12076732-12076751 MS.gene059161:CDS 40.0%
! ACAAGGGCAGTGTGAATTTT+GGG - chr1.2:12076733-12076752 MS.gene059161:CDS 40.0%
! ACCATCACCAACAACTGTTT+TGG - chr1.2:12076653-12076672 MS.gene059161:CDS 40.0%
! GGTGGAAGAGAAAGTTTTTG+TGG + chr1.2:12076411-12076430 None:intergenic 40.0%
! GTCTTTTACGACGTCGTATA+GGG - chr1.2:12076701-12076720 MS.gene059161:CDS 40.0%
! TAGGTTGTTGTCTTTGGAGA+GGG - chr1.2:12076608-12076627 MS.gene059161:CDS 40.0%
! TCCAAAACAGTTGTTGGTGA+TGG + chr1.2:12076657-12076676 None:intergenic 40.0%
! TGTCTTTTACGACGTCGTAT+AGG - chr1.2:12076700-12076719 MS.gene059161:CDS 40.0%
!! AATTTTGGGTCTAGTAGGTG+GGG - chr1.2:12076747-12076766 MS.gene059161:CDS 40.0%
!! GAATTTTGGGTCTAGTAGGT+GGG - chr1.2:12076746-12076765 MS.gene059161:CDS 40.0%
!! GTGTGAATTTTGGGTCTAGT+AGG - chr1.2:12076742-12076761 MS.gene059161:CDS 40.0%
!! TCACCAACAACTGTTTTGGA+AGG - chr1.2:12076657-12076676 MS.gene059161:CDS 40.0%
!! TGAATTTTGGGTCTAGTAGG+TGG - chr1.2:12076745-12076764 MS.gene059161:CDS 40.0%
!! TTCAAGTTGGAGTGTTGATG+GGG - chr1.2:12076818-12076837 MS.gene059161:CDS 40.0%
AAACACGGTCGTGATAACAC+TGG + chr1.2:12076432-12076451 None:intergenic 45.0%
ATCGATACCGTTTAAGTGGG+AGG - chr1.2:12076494-12076513 MS.gene059161:CDS 45.0%
CCAGAGCCAAAAGAAAGAGA+AGG - chr1.2:12076552-12076571 MS.gene059161:CDS 45.0%
CTCTCCAAAGACAACAACCT+AGG + chr1.2:12076609-12076628 None:intergenic 45.0%
GGACCTTCCAAAACAGTTGT+TGG + chr1.2:12076663-12076682 None:intergenic 45.0%
TCCAAAGACAACAACCTAGG+AGG + chr1.2:12076606-12076625 None:intergenic 45.0%
TCCTCCTAGGTTGTTGTCTT+TGG - chr1.2:12076602-12076621 MS.gene059161:CDS 45.0%
! ATCACGACCGTGTTTTTCGT+CGG - chr1.2:12076437-12076456 MS.gene059161:CDS 45.0%
! CTAGGTTGTTGTCTTTGGAG+AGG - chr1.2:12076607-12076626 MS.gene059161:CDS 45.0%
!! TCAAGTTGGAGTGTTGATGG+GGG - chr1.2:12076819-12076838 MS.gene059161:CDS 45.0%
!!! CCTTCTCTTTCTTTTGGCTC+TGG + chr1.2:12076555-12076574 None:intergenic 45.0%
!!! GTTTTGGAAGGTCCATACGT+TGG - chr1.2:12076669-12076688 MS.gene059161:CDS 45.0%
AGAAGGGGAAGCTTCTCACA+TGG - chr1.2:12076870-12076889 MS.gene059161:CDS 50.0%
ATCGATATAGCCGTCGTTCG+CGG + chr1.2:12076480-12076499 None:intergenic 50.0%
GGTCGTGTATGACATGACCT+AGG + chr1.2:12076534-12076553 None:intergenic 50.0%
GGTGCTTCCTCCCACTTAAA+CGG + chr1.2:12076504-12076523 None:intergenic 50.0%
!! GAGCTTTGGAACTTCCTCCT+AGG - chr1.2:12076589-12076608 MS.gene059161:CDS 50.0%
!! GAGTGTTGATGGGGGTGATA+AGG - chr1.2:12076827-12076846 MS.gene059161:CDS 50.0%
!! TTAAGTGGGAGGAAGCACCA+GGG - chr1.2:12076505-12076524 MS.gene059161:CDS 50.0%
!! TTTAAGTGGGAGGAAGCACC+AGG - chr1.2:12076504-12076523 MS.gene059161:CDS 50.0%
CACGGTCGTGATAACACTGG+TGG + chr1.2:12076429-12076448 None:intergenic 55.0%
GACACTGCACGTCCAACGTA+TGG + chr1.2:12076684-12076703 None:intergenic 55.0%
GGTGCCTCCGACGAAAAACA+CGG + chr1.2:12076447-12076466 None:intergenic 55.0%
TGACATGACCTAGGCTTCCC+TGG + chr1.2:12076525-12076544 None:intergenic 55.0%
TTGGGTCTAGTAGGTGGGGT+GGG - chr1.2:12076751-12076770 MS.gene059161:CDS 55.0%
TTTGGGTCTAGTAGGTGGGG+TGG - chr1.2:12076750-12076769 MS.gene059161:CDS 55.0%
! ACGACCGTGTTTTTCGTCGG+AGG - chr1.2:12076440-12076459 MS.gene059161:CDS 55.0%
AACTCCACCACCGCGAACGA+CGG - chr1.2:12076467-12076486 MS.gene059161:CDS 60.0%
GATATAGCCGTCGTTCGCGG+TGG + chr1.2:12076477-12076496 None:intergenic 60.0%
!! AGGAAGCACCAGGGAAGCCT+AGG - chr1.2:12076514-12076533 MS.gene059161:CDS 60.0%
ATAGCCGTCGTTCGCGGTGG+TGG + chr1.2:12076474-12076493 None:intergenic 65.0%
!! GTCGTTCGCGGTGGTGGAGT+TGG + chr1.2:12076468-12076487 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 12076342 12076908 12076342 ID=MS.gene059161
chr1.2 mRNA 12076342 12076908 12076342 ID=MS.gene059161.t1;Parent=MS.gene059161
chr1.2 exon 12076342 12076908 12076342 ID=MS.gene059161.t1.exon1;Parent=MS.gene059161.t1
chr1.2 CDS 12076342 12076908 12076342 ID=cds.MS.gene059161.t1;Parent=MS.gene059161.t1
Gene Sequence

>MS.gene059161

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAGTGTTATCACGACCGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCGATACCGTTTAAGTGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCATACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCACCATCACCAACAACTGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACAAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGG

Protein sequence

>MS.gene059161.t1

MTSENNKKLDHDDDELRSNYNTPQKLSLPPVLSRPCFSSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLW