AlfalfaGEDB | MS.gene74990

M. sativa cultivar XinJiangDaYe / MS.gene74990

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74990.t1	XP_013470249.1	97.3	74	2	0	1	74	162	235	1.10E-19	105.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74990.t1	Q9FNN6	65.8	38	13	0	1	38	156	193	2.6e-06	52.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74990.t1	G7ZYT0	97.3	74	2	0	1	74	162	235	7.6e-20	105.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene74990	0.801998	6.91E-49	-1.69E-46
MS.gene04920	MS.gene74990	0.80106	1.08E-48	-1.69E-46
MS.gene052533	MS.gene74990	0.816695	4.84E-52	-1.69E-46
MS.gene054893	MS.gene74990	0.803933	2.75E-49	-1.69E-46
MS.gene055150	MS.gene74990	0.833084	6.54E-56	-1.69E-46
MS.gene057102	MS.gene74990	0.812886	3.38E-51	-1.69E-46
MS.gene057148	MS.gene74990	-0.819109	1.38E-52	-1.69E-46
MS.gene058095	MS.gene74990	0.809993	1.44E-50	-1.69E-46
MS.gene05985	MS.gene74990	-0.848911	4.53E-60	-1.69E-46
MS.gene059901	MS.gene74990	-0.847185	1.36E-59	-1.69E-46
MS.gene060522	MS.gene74990	0.807441	5.05E-50	-1.69E-46
MS.gene060937	MS.gene74990	0.801276	9.72E-49	-1.69E-46
MS.gene061100	MS.gene74990	0.803468	3.44E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74990.t1	MTR_1g111830	97.297	74	2	0	1	74	162	235	1.20e-47	151
MS.gene74990.t1	MTR_1g033620	65.789	38	13	0	1	38	43	80	7.71e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74990.t1	AT5G04760	68.750	48	15	0	1	48	137	184	5.00e-15	66.6

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTTTCTTCACCGAATTC+TGG	0.216560	1.3:-75887192	None:intergenic
CTGTGCCTATTGATCAGAAC+TGG	0.289180	1.3:+75887266	MS.gene74990:CDS
CAGGATCAAATGAGTGCATA+TGG	0.389179	1.3:+75887361	MS.gene74990:CDS
GTACTTTCTTCGCCAGAATT+CGG	0.412820	1.3:+75887180	MS.gene74990:CDS
CAGAACTGGGTTCCTCCTCC+TGG	0.418709	1.3:+75887280	MS.gene74990:CDS
GGAACCCAGTTCTGATCAAT+AGG	0.426296	1.3:-75887271	None:intergenic
TTATCCGTCAAACGATTTGC+AGG	0.442120	1.3:+75887342	MS.gene74990:CDS
TGATCCTGCAAATCGTTTGA+CGG	0.446093	1.3:-75887346	None:intergenic
TGGGTTCCTCCTCCTGGTGG+TGG	0.475189	1.3:+75887286	MS.gene74990:CDS
TGTTGCATTGAACCACCACC+AGG	0.537371	1.3:-75887298	None:intergenic
AACTGGGTTCCTCCTCCTGG+TGG	0.574702	1.3:+75887283	MS.gene74990:CDS
CAGTTCTGATCAATAGGCAC+AGG	0.581958	1.3:-75887265	None:intergenic
AGGATCAAATGAGTGCATAT+GGG	0.599865	1.3:+75887362	MS.gene74990:CDS
AGAATTCGGTGAAGAAAGAG+AGG	0.605688	1.3:+75887194	MS.gene74990:CDS
TGTGCCTATTGATCAGAACT+GGG	0.607519	1.3:+75887267	MS.gene74990:CDS
ATTGAACCACCACCAGGAGG+AGG	0.621879	1.3:-75887292	None:intergenic
TATTCATGACATTACATCGG+TGG	0.643904	1.3:+75887228	MS.gene74990:CDS
AAGTATTCATGACATTACAT+CGG	0.675179	1.3:+75887225	MS.gene74990:CDS
TGCATTGAACCACCACCAGG+AGG	0.701355	1.3:-75887295	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTATTCATGACATTACAT+CGG	+	chr1.3:75887225-75887244	MS.gene74990:CDS	25.0%
	AGGATCAAATGAGTGCATAT+GGG	+	chr1.3:75887362-75887381	MS.gene74990:CDS	35.0%
	TATTCATGACATTACATCGG+TGG	+	chr1.3:75887228-75887247	MS.gene74990:CDS	35.0%
!!!	GAAGAAAGTACTTTTGAGCA+TGG	-	chr1.3:75887171-75887190	None:intergenic	35.0%
	CAGGATCAAATGAGTGCATA+TGG	+	chr1.3:75887361-75887380	MS.gene74990:CDS	40.0%
	GTACTTTCTTCGCCAGAATT+CGG	+	chr1.3:75887180-75887199	MS.gene74990:CDS	40.0%
	TCTCTTTCTTCACCGAATTC+TGG	-	chr1.3:75887195-75887214	None:intergenic	40.0%
	TGATCCTGCAAATCGTTTGA+CGG	-	chr1.3:75887349-75887368	None:intergenic	40.0%
	TGTGCCTATTGATCAGAACT+GGG	+	chr1.3:75887267-75887286	MS.gene74990:CDS	40.0%
	TTATCCGTCAAACGATTTGC+AGG	+	chr1.3:75887342-75887361	MS.gene74990:CDS	40.0%
!	AGAATTCGGTGAAGAAAGAG+AGG	+	chr1.3:75887194-75887213	MS.gene74990:CDS	40.0%
	CTGTGCCTATTGATCAGAAC+TGG	+	chr1.3:75887266-75887285	MS.gene74990:CDS	45.0%
!	CAGTTCTGATCAATAGGCAC+AGG	-	chr1.3:75887268-75887287	None:intergenic	45.0%
!	GGAACCCAGTTCTGATCAAT+AGG	-	chr1.3:75887274-75887293	None:intergenic	45.0%
	TGTTGCATTGAACCACCACC+AGG	-	chr1.3:75887301-75887320	None:intergenic	50.0%
	ATTGAACCACCACCAGGAGG+AGG	-	chr1.3:75887295-75887314	None:intergenic	55.0%
	TGCATTGAACCACCACCAGG+AGG	-	chr1.3:75887298-75887317	None:intergenic	55.0%
	AACTGGGTTCCTCCTCCTGG+TGG	+	chr1.3:75887283-75887302	MS.gene74990:CDS	60.0%
	CAGAACTGGGTTCCTCCTCC+TGG	+	chr1.3:75887280-75887299	MS.gene74990:CDS	60.0%
	TGGGTTCCTCCTCCTGGTGG+TGG	+	chr1.3:75887286-75887305	MS.gene74990:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	75887160	75887383	75887160	ID=MS.gene74990
chr1.3	mRNA	75887160	75887383	75887160	ID=MS.gene74990.t1;Parent=MS.gene74990
chr1.3	exon	75887160	75887383	75887160	ID=MS.gene74990.t1.exon1;Parent=MS.gene74990.t1
chr1.3	CDS	75887160	75887383	75887160	ID=cds.MS.gene74990.t1;Parent=MS.gene74990.t1

Gene Sequence	>MS.gene74990 GTGGCTAGCCATGCTCAAAAGTACTTTCTTCGCCAGAATTCGGTGAAGAAAGAGAGGAAGAGATCAAGTATTCATGACATTACATCGGTGGACAGTAACTCAGCTCCTGTGCCTATTGATCAGAACTGGGTTCCTCCTCCTGGTGGTGGTTCAATGCAACAACAGTCTCAAGAAATGCATCATTATCCGTCAAACGATTTGCAGGATCAAATGAGTGCATATGG
Protein sequence	>MS.gene74990.t1 VASHAQKYFLRQNSVKKERKRSSIHDITSVDSNSAPVPIDQNWVPPPGGGSMQQQSQEMHHYPSNDLQDQMSAY