Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75494.t1 | AES72186.1 | 92.6 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 6.50E-59 | 236.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75494.t1 | Q9LFT5 | 43.2 | 125 | 61 | 3 | 4 | 122 | 2 | 122 | 2.3e-20 | 99.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75494.t1 | G7J3Q1 | 92.6 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 4.7e-59 | 236.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050100 | MS.gene75494 | 0.846388 | 2.24E-59 | -1.69E-46 |
| MS.gene050298 | MS.gene75494 | 0.831455 | 1.65E-55 | -1.69E-46 |
| MS.gene050587 | MS.gene75494 | 0.827856 | 1.24E-54 | -1.69E-46 |
| MS.gene05143 | MS.gene75494 | 0.842538 | 2.43E-58 | -1.69E-46 |
| MS.gene052142 | MS.gene75494 | 0.835564 | 1.56E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene75494 | 0.862956 | 3.51E-64 | -1.69E-46 |
| MS.gene052698 | MS.gene75494 | 0.806648 | 7.43E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene75494 | 0.833381 | 5.51E-56 | -1.69E-46 |
| MS.gene053383 | MS.gene75494 | 0.825876 | 3.69E-54 | -1.69E-46 |
| MS.gene05456 | MS.gene75494 | 0.855992 | 4.35E-62 | -1.69E-46 |
| MS.gene054596 | MS.gene75494 | 0.807798 | 4.24E-50 | -1.69E-46 |
| MS.gene055359 | MS.gene75494 | 0.870901 | 1.03E-66 | -1.69E-46 |
| MS.gene058168 | MS.gene75494 | 0.837312 | 5.60E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene75494 | 0.841707 | 4.04E-58 | -1.69E-46 |
| MS.gene059632 | MS.gene75494 | 0.860047 | 2.71E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75494.t1 | MTR_3g087020 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 6.43e-78 | 226 |
| MS.gene75494.t1 | MTR_8g090010 | 57.143 | 126 | 47 | 3 | 1 | 122 | 1 | 123 | 1.06e-34 | 116 |
| MS.gene75494.t1 | MTR_3g110550 | 41.860 | 86 | 38 | 2 | 40 | 122 | 54 | 130 | 4.66e-16 | 69.3 |
| MS.gene75494.t1 | MTR_4g097720 | 42.857 | 77 | 39 | 2 | 46 | 122 | 50 | 121 | 6.47e-14 | 63.5 |
| MS.gene75494.t1 | MTR_2g099810 | 42.105 | 76 | 27 | 2 | 47 | 122 | 70 | 128 | 1.11e-13 | 63.2 |
| MS.gene75494.t1 | MTR_4g114660 | 38.824 | 85 | 38 | 2 | 39 | 122 | 43 | 114 | 4.06e-13 | 61.2 |
| MS.gene75494.t1 | MTR_2g103780 | 34.146 | 123 | 69 | 5 | 1 | 122 | 2 | 113 | 1.52e-12 | 59.7 |
| MS.gene75494.t1 | MTR_5g014840 | 37.662 | 77 | 43 | 2 | 46 | 122 | 60 | 131 | 4.71e-12 | 58.9 |
| MS.gene75494.t1 | MTR_8g017850 | 37.975 | 79 | 28 | 2 | 44 | 122 | 72 | 129 | 5.02e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75494.t1 | AT5G10310 | 47.573 | 103 | 44 | 3 | 26 | 122 | 24 | 122 | 2.08e-25 | 93.2 |
| MS.gene75494.t1 | AT5G10310 | 48.837 | 86 | 35 | 2 | 42 | 122 | 47 | 128 | 4.50e-23 | 87.4 |
Find 33 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAACCTCATCTTGTTAA+TGG | 0.240049 | 3.3:+72560346 | MS.gene75494:CDS |
| ATCCGTTATTATTATTACCT+TGG | 0.417680 | 3.3:-72560903 | None:intergenic |
| GGTTGATAGAAGCAAGAAAC+TGG | 0.429204 | 3.3:-72560401 | None:intergenic |
| GGCTGAACATGTTCATGGCT+TGG | 0.438174 | 3.3:-72560832 | None:intergenic |
| GACCTGGCTGAACATGTTCA+TGG | 0.454543 | 3.3:-72560837 | None:intergenic |
| CTCCAAGGTAATAATAATAA+CGG | 0.464837 | 3.3:+72560901 | MS.gene75494:CDS |
| TGTGTAGGGTTTATTATTTG+AGG | 0.467998 | 3.3:+72560729 | MS.gene75494:intron |
| AAGAAACTGGACACAGTAGA+AGG | 0.491502 | 3.3:-72560388 | None:intergenic |
| CAATGGCATTTCCAACTCAA+TGG | 0.495776 | 3.3:-72560940 | None:intergenic |
| AAAGAAATTACCACCTTCCA+TGG | 0.501489 | 3.3:-72560878 | None:intergenic |
| GGAACTTGCACTGCCATGCA+TGG | 0.505648 | 3.3:-72560811 | None:intergenic |
| CACTGCCTCAACTTCATCCA+TGG | 0.509432 | 3.3:+72560861 | MS.gene75494:CDS |
| AGGGAAAAGCCTACCCTTGG+AGG | 0.512819 | 3.3:-72560437 | None:intergenic |
| ATTTCTAAGGGTTGAAGAAG+TGG | 0.515036 | 3.3:-72560969 | None:intergenic |
| CAAATTACAAGCCATTGAGT+TGG | 0.518087 | 3.3:+72560929 | MS.gene75494:CDS |
| GGTGGTAATTTCTTTCTCCA+AGG | 0.542564 | 3.3:+72560886 | MS.gene75494:CDS |
| CACATTCTGCAATTCCTCCA+AGG | 0.543179 | 3.3:+72560423 | MS.gene75494:CDS |
| AGCCATGAACATGTTCAGCC+AGG | 0.557195 | 3.3:+72560835 | MS.gene75494:CDS |
| GAAGTTGAGGCAGTGTGACC+TGG | 0.560053 | 3.3:-72560853 | None:intergenic |
| ACCTTCCATGGATGAAGTTG+AGG | 0.576895 | 3.3:-72560866 | None:intergenic |
| GCCTCAACTTCATCCATGGA+AGG | 0.583985 | 3.3:+72560865 | MS.gene75494:CDS |
| ATATGAATTAAATGAAGCCA+TGG | 0.598193 | 3.3:-72560327 | None:intergenic |
| TTACATTTGTTGTGACATGT+AGG | 0.598733 | 3.3:-72560778 | None:intergenic |
| TGATGACCATTAACAAGATG+AGG | 0.607087 | 3.3:-72560352 | None:intergenic |
| CATTTGTTGTGACATGTAGG+TGG | 0.607908 | 3.3:-72560775 | None:intergenic |
| TTGAGTTGGAAATGCCATTG+TGG | 0.619909 | 3.3:+72560943 | MS.gene75494:CDS |
| TGAAGAAGTGGCCACCACAA+TGG | 0.621299 | 3.3:-72560957 | None:intergenic |
| TAACCAATGTCATCCATGCA+TGG | 0.626151 | 3.3:+72560798 | MS.gene75494:CDS |
| ACATTCTGCAATTCCTCCAA+GGG | 0.630675 | 3.3:+72560424 | MS.gene75494:CDS |
| TCAACTTCATCCATGGAAGG+TGG | 0.660618 | 3.3:+72560868 | MS.gene75494:CDS |
| CTGCCATGCATGGATGACAT+TGG | 0.662535 | 3.3:-72560801 | None:intergenic |
| CTTGGAGGAATTGCAGAATG+TGG | 0.672231 | 3.3:-72560422 | None:intergenic |
| AGTTGGAAATGCCATTGTGG+TGG | 0.687706 | 3.3:+72560946 | MS.gene75494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTAACAAACTTAATTAAC+TGG | - | chr3.3:72560706-72560725 | None:intergenic | 20.0% |
| !! | GTTTGAATTCACTATAAAAT+TGG | - | chr3.3:72560631-72560650 | None:intergenic | 20.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | - | chr3.3:72560626-72560645 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | + | chr3.3:72560346-72560365 | MS.gene75494:CDS | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | - | chr3.3:72560906-72560925 | None:intergenic | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | + | chr3.3:72560901-72560920 | MS.gene75494:CDS | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | + | chr3.3:72560740-72560759 | MS.gene75494:CDS | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | + | chr3.3:72560565-72560584 | MS.gene75494:intron | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | + | chr3.3:72560507-72560526 | MS.gene75494:intron | 25.0% |
| ACAGTAATATACTGTGATTC+TGG | - | chr3.3:72560560-72560579 | None:intergenic | 30.0% | |
| ACTTAATTAACTGGTGAGTT+TGG | - | chr3.3:72560697-72560716 | None:intergenic | 30.0% | |
| TGTGTAGGGTTTATTATTTG+AGG | + | chr3.3:72560729-72560748 | MS.gene75494:intron | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | + | chr3.3:72560491-72560510 | MS.gene75494:intron | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | - | chr3.3:72560659-72560678 | None:intergenic | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | + | chr3.3:72560715-72560734 | MS.gene75494:intron | 30.0% |
| !! | AGTTTGTTAACTATGTGTGT+AGG | + | chr3.3:72560714-72560733 | MS.gene75494:intron | 30.0% |
| !! | TTACATTTGTTGTGACATGT+AGG | - | chr3.3:72560781-72560800 | None:intergenic | 30.0% |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | + | chr3.3:72560501-72560520 | MS.gene75494:intron | 30.0% |
| AAAGAAATTACCACCTTCCA+TGG | - | chr3.3:72560881-72560900 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAATAAA+GGG | - | chr3.3:72560459-72560478 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | + | chr3.3:72560929-72560948 | MS.gene75494:CDS | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | + | chr3.3:72560654-72560673 | MS.gene75494:intron | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | - | chr3.3:72560486-72560505 | None:intergenic | 35.0% | |
| TGATGACCATTAACAAGATG+AGG | - | chr3.3:72560355-72560374 | None:intergenic | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | + | chr3.3:72560745-72560764 | MS.gene75494:CDS | 35.0% | |
| AAGAAACTGGACACAGTAGA+AGG | - | chr3.3:72560391-72560410 | None:intergenic | 40.0% | |
| ACATTCTGCAATTCCTCCAA+GGG | + | chr3.3:72560424-72560443 | MS.gene75494:CDS | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | - | chr3.3:72560471-72560490 | None:intergenic | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | - | chr3.3:72560943-72560962 | None:intergenic | 40.0% | |
| GAGTGAATGAGGGAGAATAA+AGG | - | chr3.3:72560460-72560479 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | - | chr3.3:72560470-72560489 | None:intergenic | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | + | chr3.3:72560886-72560905 | MS.gene75494:CDS | 40.0% | |
| TAACCAATGTCATCCATGCA+TGG | + | chr3.3:72560798-72560817 | MS.gene75494:CDS | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | - | chr3.3:72560686-72560705 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTAGG+TGG | - | chr3.3:72560778-72560797 | None:intergenic | 40.0% |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | - | chr3.3:72560404-72560423 | None:intergenic | 40.0% |
| !! | TTGAGTTGGAAATGCCATTG+TGG | + | chr3.3:72560943-72560962 | MS.gene75494:CDS | 40.0% |
| ACCTTCCATGGATGAAGTTG+AGG | - | chr3.3:72560869-72560888 | None:intergenic | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | + | chr3.3:72560946-72560965 | MS.gene75494:CDS | 45.0% | |
| CACATTCTGCAATTCCTCCA+AGG | + | chr3.3:72560423-72560442 | MS.gene75494:CDS | 45.0% | |
| CTTGGAGGAATTGCAGAATG+TGG | - | chr3.3:72560425-72560444 | None:intergenic | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | - | chr3.3:72560609-72560628 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | + | chr3.3:72560522-72560541 | MS.gene75494:intron | 45.0% | |
| TAAAGGGAAAAGCCTACCCT+TGG | - | chr3.3:72560443-72560462 | None:intergenic | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | + | chr3.3:72560868-72560887 | MS.gene75494:CDS | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | + | chr3.3:72560526-72560545 | MS.gene75494:intron | 45.0% | |
| AGCCATGAACATGTTCAGCC+AGG | + | chr3.3:72560835-72560854 | MS.gene75494:CDS | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | + | chr3.3:72560861-72560880 | MS.gene75494:CDS | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | - | chr3.3:72560804-72560823 | None:intergenic | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | - | chr3.3:72560840-72560859 | None:intergenic | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | + | chr3.3:72560865-72560884 | MS.gene75494:CDS | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | - | chr3.3:72560835-72560854 | None:intergenic | 50.0% | |
| TCTGCAATTCCTCCAAGGGT+AGG | + | chr3.3:72560428-72560447 | MS.gene75494:intron | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | - | chr3.3:72560960-72560979 | None:intergenic | 50.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | - | chr3.3:72560440-72560459 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | - | chr3.3:72560856-72560875 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | - | chr3.3:72560814-72560833 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72560329 | 72560987 | 72560329 | ID=MS.gene75494 |
| chr3.3 | mRNA | 72560329 | 72560987 | 72560329 | ID=MS.gene75494.t1;Parent=MS.gene75494 |
| chr3.3 | exon | 72560329 | 72560445 | 72560329 | ID=MS.gene75494.t1.exon1;Parent=MS.gene75494.t1 |
| chr3.3 | CDS | 72560329 | 72560445 | 72560329 | ID=cds.MS.gene75494.t1;Parent=MS.gene75494.t1 |
| chr3.3 | exon | 72560736 | 72560987 | 72560736 | ID=MS.gene75494.t1.exon2;Parent=MS.gene75494.t1 |
| chr3.3 | CDS | 72560736 | 72560987 | 72560736 | ID=cds.MS.gene75494.t1;Parent=MS.gene75494.t1 |
| Gene Sequence |
| Protein sequence |
