Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76435.t1 | XP_024629315.1 | 90.7 | 464 | 41 | 2 | 1 | 464 | 1 | 462 | 1.30E-232 | 815.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76435.t1 | Q557M8 | 25.8 | 395 | 203 | 13 | 8 | 379 | 14 | 341 | 1.4e-12 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76435.t1 | G7LDX0 | 86.9 | 481 | 44 | 4 | 1 | 464 | 1 | 479 | 1.4e-225 | 791.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76435 | MS.gene007951 | PPI |
| MS.gene76435 | MS.gene08242 | PPI |
| MS.gene76435 | MS.gene39443 | PPI |
| MS.gene76435 | MS.gene33782 | PPI |
| MS.gene031871 | MS.gene76435 | PPI |
| MS.gene31944 | MS.gene76435 | PPI |
| MS.gene007950 | MS.gene76435 | PPI |
| MS.gene052488 | MS.gene76435 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76435.t1 | MTR_8g077000 | 86.902 | 481 | 44 | 4 | 1 | 464 | 1 | 479 | 0.0 | 783 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76435.t1 | AT1G03280 | 57.451 | 463 | 164 | 14 | 1 | 446 | 12 | 458 | 1.99e-168 | 483 |
| MS.gene76435.t1 | AT1G03280 | 57.451 | 463 | 164 | 14 | 1 | 446 | 12 | 458 | 1.99e-168 | 483 |
| MS.gene76435.t1 | AT1G03280 | 57.451 | 463 | 164 | 14 | 1 | 446 | 12 | 458 | 1.99e-168 | 483 |
| MS.gene76435.t1 | AT4G20340 | 51.754 | 456 | 195 | 11 | 1 | 446 | 13 | 453 | 5.29e-137 | 402 |
| MS.gene76435.t1 | AT4G20340 | 51.754 | 456 | 195 | 11 | 1 | 446 | 13 | 453 | 5.29e-137 | 402 |
| MS.gene76435.t1 | AT4G20810 | 47.122 | 469 | 181 | 15 | 8 | 463 | 1 | 415 | 6.82e-127 | 375 |
| MS.gene76435.t1 | AT4G20340 | 51.834 | 409 | 173 | 10 | 48 | 446 | 4 | 398 | 8.71e-119 | 354 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.1:-30752524 | None:intergenic |
| GACTTGCCTGTTCCAAAATT+TGG | 0.170144 | 8.1:+30756467 | MS.gene76435:CDS |
| AACTGACAGTACATCTTTAA+AGG | 0.175603 | 8.1:+30757866 | MS.gene76435:CDS |
| GCTTGAAGACTGCCAAATTT+TGG | 0.179091 | 8.1:-30756479 | None:intergenic |
| TGGGAAGAGGCTCCTGTTAA+AGG | 0.269316 | 8.1:+30758357 | MS.gene76435:CDS |
| CGACCGATTTGGTCTATTAG+AGG | 0.304159 | 8.1:-30756437 | None:intergenic |
| GAAGCTCGGGCTAGTGCAGC+TGG | 0.317114 | 8.1:+30756506 | MS.gene76435:CDS |
| ATCTGCTGTGTTCACATCTT+GGG | 0.327954 | 8.1:-30758259 | None:intergenic |
| CATCTGCTGTGTTCACATCT+TGG | 0.328566 | 8.1:-30758260 | None:intergenic |
| TAATGCTGGTGATTCTAATC+TGG | 0.336277 | 8.1:+30756547 | MS.gene76435:CDS |
| GAAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.1:+30752511 | MS.gene76435:CDS |
| GTGAAATTCGTACTTATTGT+AGG | 0.350382 | 8.1:-30752302 | None:intergenic |
| GGGCTGATTATCTTCTTTAC+CGG | 0.354315 | 8.1:-30752503 | None:intergenic |
| ACGGCGGCGCGAAAAGTTAA+AGG | 0.358209 | 8.1:+30754871 | MS.gene76435:CDS |
| GCATAAGCTGAAAGATGAAT+TGG | 0.358679 | 8.1:+30754550 | MS.gene76435:CDS |
| GATTCTAATCTGGGATATAA+TGG | 0.362821 | 8.1:+30756557 | MS.gene76435:CDS |
| GAATAAAATGTACCTTTAAC+AGG | 0.371908 | 8.1:-30758369 | None:intergenic |
| TCATCTTCATTGTCTTCTGC+TGG | 0.387140 | 8.1:-30760671 | None:intergenic |
| ACTTAAGAAGCAACACGAAT+TGG | 0.389424 | 8.1:+30758218 | MS.gene76435:CDS |
| GTCTTCAAGCATGGGAAGCT+CGG | 0.396973 | 8.1:+30756492 | MS.gene76435:CDS |
| TATGTACAAATTGTGGAAAA+AGG | 0.405813 | 8.1:+30754600 | MS.gene76435:CDS |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.1:+30756521 | MS.gene76435:CDS |
| AGTCTTCTACCCGACCGATT+TGG | 0.429028 | 8.1:-30756448 | None:intergenic |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.1:-30753426 | None:intergenic |
| TGCAGATATATAAAGGGGTT+AGG | 0.445590 | 8.1:+30754507 | MS.gene76435:intron |
| TTCCATTGTGAATGTTGTAA+TGG | 0.457370 | 8.1:+30754779 | MS.gene76435:CDS |
| TCGACTGGGAAGAAGGTTGA+TGG | 0.459603 | 8.1:+30760696 | MS.gene76435:CDS |
| TTTATAGGATGAGTATATCA+AGG | 0.460980 | 8.1:+30758179 | MS.gene76435:intron |
| CGATTGGAAGTACTGCTAGA+AGG | 0.461514 | 8.1:-30758285 | None:intergenic |
| TAAAGGAAATGGAGGTTACA+AGG | 0.463278 | 8.1:+30760622 | MS.gene76435:intron |
| AGGAAAAGATGGAGAAGAAA+AGG | 0.464213 | 8.1:+30754275 | MS.gene76435:CDS |
| ATTGTCTTCTGCTGGAGCAT+CGG | 0.466214 | 8.1:-30760663 | None:intergenic |
| ACAGCGCGGAGAGGTGAAGC+AGG | 0.479424 | 8.1:+30757935 | MS.gene76435:CDS |
| AGATTATCACTAGAGTTCAT+AGG | 0.486428 | 8.1:+30753482 | MS.gene76435:CDS |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.489229 | 8.1:-30756590 | None:intergenic |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.1:+30757894 | MS.gene76435:CDS |
| AATGCTGGTGATTCTAATCT+GGG | 0.496431 | 8.1:+30756548 | MS.gene76435:CDS |
| GAATATATATGTACAAATTG+TGG | 0.502239 | 8.1:+30754593 | MS.gene76435:CDS |
| TTTCATGCCAACTTGGCGAT+TGG | 0.507113 | 8.1:-30758301 | None:intergenic |
| TCTTCAAGCATGGGAAGCTC+GGG | 0.510368 | 8.1:+30756493 | MS.gene76435:CDS |
| TGAAATTCGTACTTATTGTA+GGG | 0.520023 | 8.1:-30752301 | None:intergenic |
| ACAATGAAGATGATGTCGAC+TGG | 0.524851 | 8.1:+30760681 | MS.gene76435:CDS |
| GCTTTCTATGATGATTATAC+CGG | 0.526519 | 8.1:+30752484 | MS.gene76435:CDS |
| CAGGTGGTGGTTGACTTCAA+TGG | 0.533139 | 8.1:+30757810 | MS.gene76435:intron |
| TCATCCTTGATGCCCTCACC+AGG | 0.539237 | 8.1:+30752563 | MS.gene76435:CDS |
| ATCAGGTATGAATCTTATGA+AGG | 0.544335 | 8.1:+30757911 | MS.gene76435:CDS |
| CACTAGAGTTCATAGGAAAG+AGG | 0.545956 | 8.1:+30753489 | MS.gene76435:CDS |
| AGTACTTCCAATCGCCAAGT+TGG | 0.547271 | 8.1:+30758294 | MS.gene76435:CDS |
| AATTTGGCAGTCTTCAAGCA+TGG | 0.551091 | 8.1:+30756483 | MS.gene76435:CDS |
| AAGCCTCTAATAGACCAAAT+CGG | 0.551332 | 8.1:+30756434 | MS.gene76435:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.1:-30757897 | None:intergenic |
| ATTTGGCAGTCTTCAAGCAT+GGG | 0.552152 | 8.1:+30756484 | MS.gene76435:CDS |
| CTCTAATAGACCAAATCGGT+CGG | 0.554167 | 8.1:+30756438 | MS.gene76435:CDS |
| GATGCTACGAAAGGAAAAGA+TGG | 0.554983 | 8.1:+30754264 | MS.gene76435:CDS |
| AAGCTCGGGCTAGTGCAGCT+GGG | 0.559290 | 8.1:+30756507 | MS.gene76435:CDS |
| TGAACCTGGTGAGGGCATCA+AGG | 0.563106 | 8.1:-30752567 | None:intergenic |
| GATGATGTCGACTGGGAAGA+AGG | 0.566390 | 8.1:+30760689 | MS.gene76435:CDS |
| CAATGAAGATGATGTCGACT+GGG | 0.567076 | 8.1:+30760682 | MS.gene76435:CDS |
| TCTAATAGACCAAATCGGTC+GGG | 0.571691 | 8.1:+30756439 | MS.gene76435:CDS |
| TTGATAAATTGAACCTGGTG+AGG | 0.571718 | 8.1:-30752576 | None:intergenic |
| GCTGCTCAAGAAGGTGGAGA+TGG | 0.572571 | 8.1:+30754830 | MS.gene76435:CDS |
| AAGTTAGCTGCTCAAGAAGG+TGG | 0.576893 | 8.1:+30754824 | MS.gene76435:CDS |
| ATCAGCTGTAGCAGCTACAG+CGG | 0.579271 | 8.1:-30754244 | None:intergenic |
| AAACGCAAGGAAGATGATGA+TGG | 0.583208 | 8.1:+30758327 | MS.gene76435:CDS |
| TGATAAATTGAACCTGGTGA+GGG | 0.583295 | 8.1:-30752575 | None:intergenic |
| TCTGCTGTGTTCACATCTTG+GGG | 0.584121 | 8.1:-30758258 | None:intergenic |
| GTATCTCCGCTGTATGGCAG+TGG | 0.587254 | 8.1:-30756584 | None:intergenic |
| TGTACATATATATTCTCGAA+CGG | 0.588463 | 8.1:-30754585 | None:intergenic |
| GTGTTGCTTCTTAAGTAAAG+CGG | 0.592790 | 8.1:-30758211 | None:intergenic |
| GTCTGTGTATTGAGCTGCTG+TGG | 0.592950 | 8.1:-30757985 | None:intergenic |
| GAAAAGTTAGCTGCTCAAGA+AGG | 0.594454 | 8.1:+30754821 | MS.gene76435:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.595897 | 8.1:+30757819 | MS.gene76435:CDS |
| ATGGATTAGAGAAGAAGACT+TGG | 0.600807 | 8.1:+30753396 | MS.gene76435:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.1:+30757831 | MS.gene76435:CDS |
| CTGTTGTCTAGATTATGCAC+AGG | 0.608523 | 8.1:+30754320 | MS.gene76435:CDS |
| TGGCATGAAATCAAAACGCA+AGG | 0.615437 | 8.1:+30758314 | MS.gene76435:CDS |
| GAGGGCATCAAGGATGACTA+CGG | 0.617138 | 8.1:-30752557 | None:intergenic |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.1:+30756533 | MS.gene76435:CDS |
| GAGATGAGAATGCAAGAAGA+CGG | 0.619322 | 8.1:+30754852 | MS.gene76435:CDS |
| AGATGATGATGGTACTGAGT+GGG | 0.622629 | 8.1:+30758338 | MS.gene76435:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.625307 | 8.1:+30753402 | MS.gene76435:CDS |
| TGATGGTACTGAGTGGGAAG+AGG | 0.628964 | 8.1:+30758344 | MS.gene76435:CDS |
| AGGAAATGGAGGTTACAAGG+TGG | 0.630329 | 8.1:+30760625 | MS.gene76435:intron |
| GCCATACAGCGGAGATACCA+AGG | 0.630579 | 8.1:+30756589 | MS.gene76435:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.1:+30757825 | MS.gene76435:CDS |
| TTCCATTACAACATTCACAA+TGG | 0.633215 | 8.1:-30754781 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.1:+30757830 | MS.gene76435:CDS |
| GCTACAGCTGATGCTACGAA+AGG | 0.657325 | 8.1:+30754255 | MS.gene76435:CDS |
| TATGAAGGAACAGCGCGGAG+AGG | 0.686569 | 8.1:+30757926 | MS.gene76435:CDS |
| ATGAGAATGCAAGAAGACGG+CGG | 0.703092 | 8.1:+30754855 | MS.gene76435:CDS |
| GGAGCACCACTGCCATACAG+CGG | 0.717941 | 8.1:+30756578 | MS.gene76435:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.1:+30752529 | MS.gene76435:CDS |
| ATGTTGTAATGGAAAACTAG+AGG | 0.731298 | 8.1:+30754790 | MS.gene76435:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.1:-30757894 | None:intergenic |
| ACAACGCTTTGGATGCACTG+CGG | 0.757151 | 8.1:+30754729 | MS.gene76435:CDS |
| AAGATGATGATGGTACTGAG+TGG | 0.777201 | 8.1:+30758337 | MS.gene76435:CDS |
| AATCTTATGAAGGAACAGCG+CGG | 0.807672 | 8.1:+30757921 | MS.gene76435:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 30752292 | 30760715 | 30752292 | ID=MS.gene76435 |
| chr8.1 | mRNA | 30752292 | 30760715 | 30752292 | ID=MS.gene76435.t1;Parent=MS.gene76435 |
| chr8.1 | exon | 30752292 | 30752311 | 30752292 | ID=MS.gene76435.t1.exon1;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30752292 | 30752311 | 30752292 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30752462 | 30752584 | 30752462 | ID=MS.gene76435.t1.exon2;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30752462 | 30752584 | 30752462 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30753390 | 30753510 | 30753390 | ID=MS.gene76435.t1.exon3;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30753390 | 30753510 | 30753390 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30754216 | 30754341 | 30754216 | ID=MS.gene76435.t1.exon4;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30754216 | 30754341 | 30754216 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30754512 | 30754621 | 30754512 | ID=MS.gene76435.t1.exon5;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30754512 | 30754621 | 30754512 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30754727 | 30754916 | 30754727 | ID=MS.gene76435.t1.exon6;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30754727 | 30754916 | 30754727 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30756425 | 30756610 | 30756425 | ID=MS.gene76435.t1.exon7;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30756425 | 30756610 | 30756425 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30757813 | 30758049 | 30757813 | ID=MS.gene76435.t1.exon8;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30757813 | 30758049 | 30757813 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30758186 | 30758378 | 30758186 | ID=MS.gene76435.t1.exon9;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30758186 | 30758378 | 30758186 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| chr8.1 | exon | 30760627 | 30760715 | 30760627 | ID=MS.gene76435.t1.exon10;Parent=MS.gene76435.t1 |
| chr8.1 | CDS | 30760627 | 30760715 | 30760627 | ID=cds.MS.gene76435.t1;Parent=MS.gene76435.t1 |
| Gene Sequence |
| Protein sequence |
