AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76529

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76529.t1 MTR_2g101330 96.667 330 10 1 1 329 1 330 0.0 662
MS.gene76529.t1 MTR_3g005210 43.354 316 171 6 5 316 6 317 1.15e-86 263
MS.gene76529.t1 MTR_5g056600 43.218 317 175 4 6 319 5 319 3.79e-85 259
MS.gene76529.t1 MTR_3g005170 42.368 321 177 6 5 321 10 326 7.00e-83 254
MS.gene76529.t1 MTR_8g062110 42.500 320 167 6 5 316 9 319 4.27e-81 249
MS.gene76529.t1 MTR_8g062110 42.500 320 167 6 5 316 18 328 9.67e-81 249
MS.gene76529.t1 MTR_0164s0070 90.840 131 12 0 1 131 1 131 1.22e-80 244
MS.gene76529.t1 MTR_0164s0070 83.099 71 12 0 206 276 122 192 9.15e-37 131
MS.gene76529.t1 MTR_1g022440 39.875 321 184 6 5 319 8 325 2.43e-79 245
MS.gene76529.t1 MTR_3g005170 45.956 272 143 4 5 275 10 278 2.64e-79 243
MS.gene76529.t1 MTR_8g062440 41.066 319 179 5 5 319 8 321 8.36e-79 243
MS.gene76529.t1 MTR_8g062440 41.250 320 178 6 5 319 8 322 4.19e-78 242
MS.gene76529.t1 MTR_5g072620 40.248 323 187 4 1 320 1 320 2.87e-77 239
MS.gene76529.t1 MTR_4g009040 40.625 320 178 5 4 319 12 323 8.45e-77 238
MS.gene76529.t1 MTR_8g062110 44.891 274 138 4 5 273 9 274 4.75e-76 235
MS.gene76529.t1 MTR_8g062110 44.891 274 138 4 5 273 9 274 8.23e-76 234
MS.gene76529.t1 MTR_8g062440 44.444 270 145 3 5 273 8 273 7.05e-75 233
MS.gene76529.t1 MTR_1g022445 38.344 326 190 7 1 319 4 325 8.03e-75 234
MS.gene76529.t1 MTR_4g009040 40.123 324 178 6 4 319 12 327 1.87e-74 233
MS.gene76529.t1 MTR_4g077130 40.506 316 180 6 5 316 9 320 4.94e-74 231
MS.gene76529.t1 MTR_4g077100 39.936 313 180 6 5 313 11 319 5.14e-74 231
MS.gene76529.t1 MTR_4g077100 42.804 271 151 4 5 274 11 278 2.93e-72 225
MS.gene76529.t1 MTR_2g104960 40.867 323 180 5 5 324 20 334 1.75e-71 225
MS.gene76529.t1 MTR_4g092080 38.110 328 189 8 2 317 11 336 5.71e-70 221
MS.gene76529.t1 MTR_3g031650 37.812 320 193 4 1 317 1 317 6.47e-70 221
MS.gene76529.t1 MTR_4g009690 40.439 319 179 5 4 319 12 322 1.66e-69 219
MS.gene76529.t1 MTR_4g006940 39.506 324 185 5 2 322 14 329 4.32e-69 219
MS.gene76529.t1 MTR_7g074880 39.877 326 180 10 4 319 7 326 7.49e-69 218
MS.gene76529.t1 MTR_7g074730 39.450 327 182 10 4 319 7 328 1.07e-68 218
MS.gene76529.t1 MTR_3g031650 40.364 275 162 2 1 275 1 273 1.12e-68 216
MS.gene76529.t1 MTR_4g009690 40.000 320 180 5 4 319 12 323 2.20e-68 217
MS.gene76529.t1 MTR_7g074820 39.939 328 180 10 4 319 7 329 5.11e-68 216
MS.gene76529.t1 MTR_7g074850 38.720 328 184 9 4 319 7 329 1.90e-67 214
MS.gene76529.t1 MTR_3g005170 37.383 321 162 6 5 321 10 295 7.79e-65 207
MS.gene76529.t1 MTR_7g074710 39.877 326 180 9 4 319 7 326 1.76e-64 207
MS.gene76529.t1 MTR_4g006940 41.392 273 152 4 2 274 14 278 4.62e-61 196
MS.gene76529.t1 MTR_4g006940 41.392 273 152 4 2 274 14 278 4.62e-61 196
MS.gene76529.t1 MTR_2g028620 35.671 328 193 9 2 319 7 326 1.38e-60 197
MS.gene76529.t1 MTR_4g081440 35.608 337 190 9 4 319 19 349 2.59e-60 197
MS.gene76529.t1 MTR_4g081440 35.988 339 187 11 4 319 23 354 5.82e-59 193
MS.gene76529.t1 MTR_2g028620 38.768 276 161 5 2 273 7 278 1.46e-58 191
MS.gene76529.t1 MTR_4g023730 33.742 326 201 8 5 319 8 329 1.26e-52 176
MS.gene76529.t1 MTR_7g074870 33.898 295 172 10 38 319 4 288 1.30e-45 157
MS.gene76529.t1 MTR_8g062110 35.931 231 132 5 94 316 11 233 3.76e-41 144
MS.gene76529.t1 MTR_8g062110 37.838 185 103 3 94 273 11 188 3.99e-36 129
MS.gene76529.t1 MTR_5g029990 30.827 266 168 5 3 267 10 260 2.78e-34 127
MS.gene76529.t1 MTR_7g117190 30.564 337 211 11 4 319 6 340 4.72e-34 128
MS.gene76529.t1 MTR_6g406250 28.716 296 197 7 5 292 55 344 1.47e-31 121
MS.gene76529.t1 MTR_7g117190 31.169 308 187 11 4 291 6 308 1.65e-31 120
MS.gene76529.t1 MTR_2g013230 28.319 339 219 9 4 321 7 342 3.83e-31 120
MS.gene76529.t1 MTR_2g013250 27.914 326 211 8 4 308 6 328 8.41e-31 119
MS.gene76529.t1 MTR_2g013260 28.354 328 207 9 4 308 7 329 3.55e-28 112
MS.gene76529.t1 MTR_2g013260 29.452 292 179 8 4 273 7 293 3.31e-27 108
MS.gene76529.t1 MTR_6g406250 31.771 192 127 2 5 193 55 245 3.57e-25 102
MS.gene76529.t1 MTR_3g011130 40.517 116 67 2 97 210 30 145 6.14e-19 82.4
MS.gene76529.t1 MTR_0685s0030 31.013 158 103 4 159 313 2 156 1.29e-18 82.0
MS.gene76529.t1 MTR_0685s0030 34.483 116 74 2 159 274 2 115 6.66e-17 75.9
MS.gene76529.t1 MTR_8g062710 42.105 95 44 2 4 98 19 102 1.04e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76529.t1 AT4G35420 70.978 317 91 1 4 320 7 322 7.94e-175 488
MS.gene76529.t1 AT1G68540 42.587 317 177 4 7 319 6 321 2.29e-83 255
MS.gene76529.t1 AT1G51410 43.614 321 175 5 2 319 6 323 8.27e-83 254
MS.gene76529.t1 AT5G19440 42.767 318 172 5 5 317 10 322 6.17e-81 249
MS.gene76529.t1 AT5G58490 41.379 319 181 4 5 320 9 324 1.11e-78 243
MS.gene76529.t1 AT1G09480 41.139 316 178 5 5 316 8 319 1.20e-78 243
MS.gene76529.t1 AT1G09480 41.139 316 178 5 5 316 55 366 2.40e-78 244
MS.gene76529.t1 AT5G42800 39.815 324 186 7 2 319 5 325 6.69e-76 238
MS.gene76529.t1 AT1G25460 43.396 318 171 7 7 319 6 319 7.17e-76 236
MS.gene76529.t1 AT1G09490 40.635 315 181 4 5 316 8 319 1.62e-75 235
MS.gene76529.t1 AT1G66800 40.764 314 178 5 5 316 8 315 9.52e-75 233
MS.gene76529.t1 AT1G09510 41.270 315 179 5 5 316 8 319 2.76e-74 232
MS.gene76529.t1 AT2G02400 38.994 318 186 5 5 319 6 318 1.79e-73 229
MS.gene76529.t1 AT1G09500 38.801 317 187 4 5 316 8 322 2.22e-71 224
MS.gene76529.t1 AT1G09500 41.852 270 154 2 5 273 8 275 5.99e-70 219
MS.gene76529.t1 AT1G61720 38.650 326 186 7 4 317 12 335 1.15e-68 218
MS.gene76529.t1 AT2G33590 38.387 310 188 3 4 313 8 314 2.23e-64 206
MS.gene76529.t1 AT1G15950 38.994 318 183 6 5 319 13 322 3.54e-63 204
MS.gene76529.t1 AT1G15950 38.994 318 183 6 5 319 13 322 3.54e-63 204
MS.gene76529.t1 AT1G76470 39.938 323 187 5 1 319 3 322 6.09e-63 203
MS.gene76529.t1 AT1G80820 37.578 322 190 6 5 323 8 321 1.57e-62 202
MS.gene76529.t1 AT2G33600 38.065 310 189 3 4 313 8 314 4.80e-60 195
MS.gene76529.t1 AT1G15950 38.762 307 177 6 5 308 13 311 2.37e-59 194
MS.gene76529.t1 AT1G09490 37.722 281 169 4 39 316 11 288 1.39e-58 191
MS.gene76529.t1 AT2G45400 36.364 330 190 7 5 319 40 364 1.09e-57 191
MS.gene76529.t1 AT1G15950 40.659 273 154 5 5 277 13 277 2.45e-56 185
MS.gene76529.t1 AT1G15950 40.217 276 157 5 5 280 13 280 9.69e-56 183
MS.gene76529.t1 AT1G15950 40.741 270 152 5 5 274 13 274 1.33e-55 182
MS.gene76529.t1 AT1G09500 36.396 283 173 4 39 316 8 288 4.04e-55 182
MS.gene76529.t1 AT4G27250 33.429 347 198 10 6 326 14 353 5.58e-54 181
MS.gene76529.t1 AT2G45400 37.410 278 165 4 5 274 40 316 9.02e-54 179
MS.gene76529.t1 AT1G80820 39.259 270 156 5 5 274 8 269 1.31e-53 177
MS.gene76529.t1 AT4G27250 33.529 340 197 10 6 326 14 343 1.94e-53 179
MS.gene76529.t1 AT1G68540 40.000 215 124 4 109 319 1 214 3.37e-47 159
MS.gene76529.t1 AT2G45400 33.108 296 180 6 37 319 17 307 6.88e-44 153
MS.gene76529.t1 AT2G23910 27.839 273 188 5 6 277 11 275 5.33e-38 137
MS.gene76529.t1 AT2G45400 37.963 216 125 4 5 212 40 254 7.63e-38 135
MS.gene76529.t1 AT2G23910 28.148 270 185 5 6 274 11 272 1.50e-37 135
MS.gene76529.t1 AT2G23910 28.000 275 189 5 6 279 11 277 1.94e-37 136
MS.gene76529.t1 AT1G15950 34.071 226 144 3 97 319 1 224 3.52e-34 125
MS.gene76529.t1 AT4G30470 26.909 275 189 6 6 278 11 275 3.61e-31 119
MS.gene76529.t1 AT2G23910 25.896 251 177 5 30 279 1 243 3.18e-28 110
MS.gene76529.t1 AT5G14700 28.788 264 173 6 5 258 55 313 1.16e-26 108
MS.gene76529.t1 AT5G14700 28.704 216 143 4 5 213 55 266 2.85e-19 86.7
MS.gene76529.t1 AT4G33360 25.993 277 180 10 4 274 14 271 1.18e-13 70.9
MS.gene76529.t1 AT4G33360 25.271 277 182 9 4 274 2 259 1.32e-13 70.9
MS.gene76529.t1 AT4G33360 25.271 277 182 9 4 274 14 271 1.67e-13 70.9

Find 69 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGAAAGTTGAACATTTA+TGG 0.118270 2.3:+2700787 MS.gene76529:CDS
ATTGGAACAGTTAGAGATTT+AGG 0.193478 2.3:+2700667 MS.gene76529:CDS
TTGTCTGGATATCATGTTAT+TGG 0.201550 2.3:+2700649 MS.gene76529:CDS
ACTAGTCCAAGCTGATTTAA+TGG 0.234242 2.3:+2700839 MS.gene76529:CDS
CATAAATCTGTTGGTAAGTT+AGG 0.314397 2.3:-2701602 None:intergenic
CTTTGCATCGTTTGTGGAGC+AGG 0.328688 2.3:+2702469 MS.gene76529:CDS
ACTGGAAAGCTGGAGAGTTT+AGG 0.349228 2.3:+2702407 MS.gene76529:CDS
AGTTGAACATTTATGGAAGT+TGG 0.360831 2.3:+2700794 MS.gene76529:CDS
GTGTGTGTGACAGGAGCTTC+AGG 0.361806 2.3:+2700592 MS.gene76529:CDS
CTGCCAGTGTCTTTGACATT+GGG 0.362452 2.3:-2701505 None:intergenic
AATAATGGATAATGATGATT+TGG 0.363243 2.3:+2701872 MS.gene76529:CDS
TCTGCCAGTGTCTTTGACAT+TGG 0.367364 2.3:-2701506 None:intergenic
TAGCTTCGACAACGCGATCA+TGG 0.382636 2.3:+2700869 MS.gene76529:CDS
CGAGTCATCTTGGAGCTCAT+TGG 0.428944 2.3:+2701256 MS.gene76529:CDS
TCAGGTTCAAGAAACTAGAT+AGG 0.430756 2.3:+2702366 MS.gene76529:intron
TGAACCAACCCTTTGGGAAT+GGG 0.449618 2.3:-2701927 None:intergenic
TTTCTTCCATTAAATCAGCT+TGG 0.450929 2.3:-2700845 None:intergenic
TGATTGCTTTGCATCGTTTG+TGG 0.452335 2.3:+2702463 MS.gene76529:CDS
CTCCAAGATGACTCGTCTAA+TGG 0.454787 2.3:-2701248 None:intergenic
TAACACAGCACGGGGTTGGT+GGG 0.455832 2.3:-2702507 None:intergenic
TACCATTAGACGAGTCATCT+TGG 0.465869 2.3:+2701246 MS.gene76529:CDS
TGTCTTTCACATTGCCTCTC+CGG 0.466059 2.3:+2700902 MS.gene76529:CDS
AGAAATTTCAATGGCATGGA+AGG 0.468896 2.3:+2701757 MS.gene76529:CDS
ATGAACCAACCCTTTGGGAA+TGG 0.471597 2.3:-2701928 None:intergenic
GAAGCATGAACCAACCCTTT+GGG 0.473604 2.3:-2701933 None:intergenic
AACACAACTCGAACAAGGGC+TGG 0.477786 2.3:-2701173 None:intergenic
TGAACTAAACACTGGAAAGC+TGG 0.486679 2.3:+2702397 MS.gene76529:CDS
AGCTTCGACAACGCGATCAT+GGG 0.489980 2.3:+2700870 MS.gene76529:CDS
ATGCTAGCAAATCGTTATCC+TGG 0.493557 2.3:+2701900 MS.gene76529:CDS
TTCAGCGTACCTTGGACCGC+TGG 0.497416 2.3:-2701125 None:intergenic
TGGGAATATTGCAAAGAGAA+TGG 0.500498 2.3:+2701539 MS.gene76529:CDS
ATTTCAATGGCATGGAAGGA+TGG 0.500563 2.3:+2701761 MS.gene76529:CDS
TTAGGTCCAATTATGAATGA+TGG 0.503563 2.3:-2701584 None:intergenic
TTTCAATGGCATGGAAGGAT+GGG 0.514191 2.3:+2701762 MS.gene76529:CDS
ACTGAGAAATTTCAATGGCA+TGG 0.516718 2.3:+2701753 MS.gene76529:CDS
ACAATAACACAGCACGGGGT+TGG 0.517165 2.3:-2702511 None:intergenic
AACACAGCACGGGGTTGGTG+GGG 0.518483 2.3:-2702506 None:intergenic
CCAGTGTTTAGTTCATAATG+TGG 0.519090 2.3:-2702389 None:intergenic
TCACATATCAAATGATCCTA+AGG 0.523302 2.3:+2700932 MS.gene76529:CDS
ATAACACAGCACGGGGTTGG+TGG 0.523703 2.3:-2702508 None:intergenic
TCCACTGCATCTGATGTGTT+AGG 0.524578 2.3:+2701626 MS.gene76529:CDS
CAAAGAGAATGGAATAGACT+TGG 0.535237 2.3:+2701550 MS.gene76529:CDS
GAACATTTATGGAAGTTGGA+AGG 0.542146 2.3:+2700798 MS.gene76529:CDS
TGATTACAATAACACAGCAC+GGG 0.544650 2.3:-2702516 None:intergenic
TTTGCATCGTTTGTGGAGCA+GGG 0.545246 2.3:+2702470 MS.gene76529:CDS
GAACACAAAGTGTGTGTGAC+AGG 0.547699 2.3:+2700583 MS.gene76529:CDS
CCTTTGGGAATGGGAAAACC+AGG 0.548463 2.3:-2701918 None:intergenic
TTTGATATGTGATTGAGTAC+CGG 0.548510 2.3:-2700921 None:intergenic
TATGAAAACAAAGCCTCTCA+TGG 0.552465 2.3:+2701828 MS.gene76529:CDS
GCAAAACATTCAGCGTACCT+TGG 0.565410 2.3:-2701133 None:intergenic
GGCAAACTGAGAAATTTCAA+TGG 0.566240 2.3:+2701748 MS.gene76529:CDS
AGGCTAACACAACTCGAACA+AGG 0.571304 2.3:-2701178 None:intergenic
CCACATTATGAACTAAACAC+TGG 0.581273 2.3:+2702389 MS.gene76529:CDS
ATCTTGGAACCAGCGGTCCA+AGG 0.581499 2.3:+2701116 MS.gene76529:CDS
AACGCGATCATGGGATGCAA+AGG 0.582374 2.3:+2700879 MS.gene76529:CDS
GTTGGTGGGGAAGAGTGAGA+TGG 0.585295 2.3:-2702493 None:intergenic
GCAGTGGAACATAAATCTGT+TGG 0.588541 2.3:-2701611 None:intergenic
GCCTAACACATCAGATGCAG+TGG 0.588657 2.3:-2701627 None:intergenic
GATGTGTTAGGCTTGTTCAA+AGG 0.590361 2.3:+2701638 MS.gene76529:CDS
GGCTAACACAACTCGAACAA+GGG 0.595366 2.3:-2701177 None:intergenic
AGGATAACGATTTGCTAGCA+TGG 0.597463 2.3:-2701898 None:intergenic
TTGATTACAATAACACAGCA+CGG 0.605506 2.3:-2702517 None:intergenic
GGAATTGTGTGAGAAACTCC+AGG 0.606759 2.3:+2701277 MS.gene76529:CDS
GCAAAGGTATCTACCATGAG+AGG 0.637555 2.3:-2701841 None:intergenic
GGCAGAAATCTTGGAACCAG+CGG 0.644848 2.3:+2701109 MS.gene76529:intron
GTACCCAATGTCAAAGACAC+TGG 0.656150 2.3:+2701502 MS.gene76529:CDS
GATTACAATAACACAGCACG+GGG 0.703785 2.3:-2702515 None:intergenic
TCTAACAGCTGAAGACGATG+AGG 0.704563 2.3:-2701198 None:intergenic
TATGTGATTGAGTACCGGAG+AGG 0.740188 2.3:-2700916 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCATTTATGTTATTCAT+AGG + chr2.3:2700961-2700980 MS.gene76529:intron 15.0%
!! GATTAATTTCTTTATATCAA+TGG - chr2.3:2702269-2702288 None:intergenic 15.0%
!! GATTAATTTCTTTATATTGA+TGG - chr2.3:2702181-2702200 None:intergenic 15.0%
!!! ATACTATAATGTTATTTGAT+TGG + chr2.3:2702128-2702147 MS.gene76529:intron 15.0%
!!! CATATACTTTTTTTTACAAA+TGG + chr2.3:2701433-2701452 MS.gene76529:intron 15.0%
!!! TTATACATATATGATTAGTT+AGG - chr2.3:2701312-2701331 None:intergenic 15.0%
!! ATATCTTTGTATGATTGATT+TGG + chr2.3:2702340-2702359 MS.gene76529:intron 20.0%
!! ATATTGCTAATTAAACTAAC+AGG + chr2.3:2701726-2701745 MS.gene76529:intron 20.0%
!! ATGTAAACACAAAAAACTAA+TGG + chr2.3:2702315-2702334 MS.gene76529:intron 20.0%
!! ATTAATCACATCACAAATAT+TGG + chr2.3:2702282-2702301 MS.gene76529:intron 20.0%
!! TATTGCTAATTAAACTAACA+GGG + chr2.3:2701727-2701746 MS.gene76529:intron 20.0%
!! TGTAAACACAAAAAACTAAT+GGG + chr2.3:2702316-2702335 MS.gene76529:intron 20.0%
!!! AATAATGGATAATGATGATT+TGG + chr2.3:2701872-2701891 MS.gene76529:CDS 20.0%
!!! ATTCTATGATGTTATTTGAT+TGG + chr2.3:2702219-2702238 MS.gene76529:intron 20.0%
!!! TGTTAGTTTAATTAGCAATA+TGG - chr2.3:2701727-2701746 None:intergenic 20.0%
! GACAAACTTGTATTGAATTA+AGG + chr2.3:2701475-2701494 MS.gene76529:intron 25.0%
! TAAATGAAATATCACACCTT+AGG - chr2.3:2700951-2700970 None:intergenic 25.0%
! TAATCAAAATCTTCACATCA+TGG + chr2.3:2701017-2701036 MS.gene76529:intron 25.0%
! TATAGATACTAACTATGCTA+AGG - chr2.3:2701965-2701984 None:intergenic 25.0%
! TGATTAATGTATGAGCTTAT+AGG - chr2.3:2701003-2701022 None:intergenic 25.0%
!! AGATCATGAAATGTATTTTG+AGG + chr2.3:2701408-2701427 MS.gene76529:intron 25.0%
!! CAATTTTTGTCCAAAAAAAC+AGG + chr2.3:2701088-2701107 MS.gene76529:intron 25.0%
!! GTTTTACCATCATTCATAAT+TGG + chr2.3:2701578-2701597 MS.gene76529:CDS 25.0%
!!! ACATTTTTTTATACACTTGC+AGG + chr2.3:2700759-2700778 MS.gene76529:intron 25.0%
AAAAAAACAGGCAGAAATCT+TGG + chr2.3:2701100-2701119 MS.gene76529:intron 30.0%
ACTTGTATTGAATTAAGGCA+TGG + chr2.3:2701480-2701499 MS.gene76529:intron 30.0%
AGCAGAAAGTTGAACATTTA+TGG + chr2.3:2700787-2700806 MS.gene76529:CDS 30.0%
AGTTGAACATTTATGGAAGT+TGG + chr2.3:2700794-2700813 MS.gene76529:CDS 30.0%
ATTGGAACAGTTAGAGATTT+AGG + chr2.3:2700667-2700686 MS.gene76529:CDS 30.0%
CCTTTGCAGTTCAAAAATAA+TGG + chr2.3:2701857-2701876 MS.gene76529:CDS 30.0%
TCACATATCAAATGATCCTA+AGG + chr2.3:2700932-2700951 MS.gene76529:CDS 30.0%
TTAGGTCCAATTATGAATGA+TGG - chr2.3:2701587-2701606 None:intergenic 30.0%
TTGTCTGGATATCATGTTAT+TGG + chr2.3:2700649-2700668 MS.gene76529:CDS 30.0%
TTTCTTCCATTAAATCAGCT+TGG - chr2.3:2700848-2700867 None:intergenic 30.0%
TTTGATATGTGATTGAGTAC+CGG - chr2.3:2700924-2700943 None:intergenic 30.0%
! CATAAATCTGTTGGTAAGTT+AGG - chr2.3:2701605-2701624 None:intergenic 30.0%
!!! AAGATTTCTGCCTGTTTTTT+TGG - chr2.3:2701101-2701120 None:intergenic 30.0%
!!! AGGCTTTGTTTTCATAAAGA+AGG - chr2.3:2701824-2701843 None:intergenic 30.0%
!!! CCATTATTTTTGAACTGCAA+AGG - chr2.3:2701860-2701879 None:intergenic 30.0%
!!! GTATGATTGATTTGGTTTTC+AGG + chr2.3:2702348-2702367 MS.gene76529:intron 30.0%
ACTGAGAAATTTCAATGGCA+TGG + chr2.3:2701753-2701772 MS.gene76529:CDS 35.0%
AGAAATTTCAATGGCATGGA+AGG + chr2.3:2701757-2701776 MS.gene76529:CDS 35.0%
CAAAGAGAATGGAATAGACT+TGG + chr2.3:2701550-2701569 MS.gene76529:CDS 35.0%
CCACATTATGAACTAAACAC+TGG + chr2.3:2702389-2702408 MS.gene76529:CDS 35.0%
GAACATTTATGGAAGTTGGA+AGG + chr2.3:2700798-2700817 MS.gene76529:CDS 35.0%
GGCAAACTGAGAAATTTCAA+TGG + chr2.3:2701748-2701767 MS.gene76529:CDS 35.0%
TACATGCACACAAATGTTCT+AGG + chr2.3:2701661-2701680 MS.gene76529:intron 35.0%
TATGAAAACAAAGCCTCTCA+TGG + chr2.3:2701828-2701847 MS.gene76529:CDS 35.0%
TCAGGTTCAAGAAACTAGAT+AGG + chr2.3:2702366-2702385 MS.gene76529:intron 35.0%
TGGGAATATTGCAAAGAGAA+TGG + chr2.3:2701539-2701558 MS.gene76529:CDS 35.0%
TTAGTTAGGAAATGTGTACC+TGG - chr2.3:2701298-2701317 None:intergenic 35.0%
! ACTAGTCCAAGCTGATTTAA+TGG + chr2.3:2700839-2700858 MS.gene76529:CDS 35.0%
! ATCAAGAGACTTCTTTTGTC+TGG + chr2.3:2700634-2700653 MS.gene76529:CDS 35.0%
! CCAGTGTTTAGTTCATAATG+TGG - chr2.3:2702392-2702411 None:intergenic 35.0%
ATGCTAGCAAATCGTTATCC+TGG + chr2.3:2701900-2701919 MS.gene76529:CDS 40.0%
ATTTCAATGGCATGGAAGGA+TGG + chr2.3:2701761-2701780 MS.gene76529:CDS 40.0%
GCAGTGGAACATAAATCTGT+TGG - chr2.3:2701614-2701633 None:intergenic 40.0%
TACCATTAGACGAGTCATCT+TGG + chr2.3:2701246-2701265 MS.gene76529:CDS 40.0%
TGAACTAAACACTGGAAAGC+TGG + chr2.3:2702397-2702416 MS.gene76529:CDS 40.0%
TGATTGCTTTGCATCGTTTG+TGG + chr2.3:2702463-2702482 MS.gene76529:CDS 40.0%
TTTCAATGGCATGGAAGGAT+GGG + chr2.3:2701762-2701781 MS.gene76529:CDS 40.0%
! GATGTGTTAGGCTTGTTCAA+AGG + chr2.3:2701638-2701657 MS.gene76529:CDS 40.0%
!! AGGATAACGATTTGCTAGCA+TGG - chr2.3:2701901-2701920 None:intergenic 40.0%
ACTGGAAAGCTGGAGAGTTT+AGG + chr2.3:2702407-2702426 MS.gene76529:CDS 45.0%
ACTGGCAGAAAAAGCAGCTT+GGG + chr2.3:2701520-2701539 MS.gene76529:CDS 45.0%
AGGCTAACACAACTCGAACA+AGG - chr2.3:2701181-2701200 None:intergenic 45.0%
CTCCAAGATGACTCGTCTAA+TGG - chr2.3:2701251-2701270 None:intergenic 45.0%
CTGCCAGTGTCTTTGACATT+GGG - chr2.3:2701508-2701527 None:intergenic 45.0%
GAACACAAAGTGTGTGTGAC+AGG + chr2.3:2700583-2700602 MS.gene76529:CDS 45.0%
GAAGCATGAACCAACCCTTT+GGG - chr2.3:2701936-2701955 None:intergenic 45.0%
GCAAAACATTCAGCGTACCT+TGG - chr2.3:2701136-2701155 None:intergenic 45.0%
GGAATTGTGTGAGAAACTCC+AGG + chr2.3:2701277-2701296 MS.gene76529:CDS 45.0%
GGCTAACACAACTCGAACAA+GGG - chr2.3:2701180-2701199 None:intergenic 45.0%
GTACCCAATGTCAAAGACAC+TGG + chr2.3:2701502-2701521 MS.gene76529:CDS 45.0%
TCCACTGCATCTGATGTGTT+AGG + chr2.3:2701626-2701645 MS.gene76529:CDS 45.0%
TCTAACAGCTGAAGACGATG+AGG - chr2.3:2701201-2701220 None:intergenic 45.0%
TCTGCCAGTGTCTTTGACAT+TGG - chr2.3:2701509-2701528 None:intergenic 45.0%
TGTCTTTCACATTGCCTCTC+CGG + chr2.3:2700902-2700921 MS.gene76529:CDS 45.0%
TTTGCATCGTTTGTGGAGCA+GGG + chr2.3:2702470-2702489 MS.gene76529:CDS 45.0%
! AGAAGCATGAACCAACCCTT+TGG - chr2.3:2701937-2701956 None:intergenic 45.0%
! ATGAACCAACCCTTTGGGAA+TGG - chr2.3:2701931-2701950 None:intergenic 45.0%
! TGAACCAACCCTTTGGGAAT+GGG - chr2.3:2701930-2701949 None:intergenic 45.0%
! TTTTCCCATTCCCAAAGGGT+TGG + chr2.3:2701923-2701942 MS.gene76529:intron 45.0%
!! CTGGTTTTCCCATTCCCAAA+GGG + chr2.3:2701919-2701938 MS.gene76529:CDS 45.0%
!! GCAAAGGTATCTACCATGAG+AGG - chr2.3:2701844-2701863 None:intergenic 45.0%
!! TATGTGATTGAGTACCGGAG+AGG - chr2.3:2700919-2700938 None:intergenic 45.0%
!!! CTTCAGGCTTTTTGGCTTCT+TGG + chr2.3:2700608-2700627 MS.gene76529:CDS 45.0%
AACACAACTCGAACAAGGGC+TGG - chr2.3:2701176-2701195 None:intergenic 50.0%
AACGCGATCATGGGATGCAA+AGG + chr2.3:2700879-2700898 MS.gene76529:CDS 50.0%
ACAATAACACAGCACGGGGT+TGG - chr2.3:2702514-2702533 None:intergenic 50.0%
AGCTTCGACAACGCGATCAT+GGG + chr2.3:2700870-2700889 MS.gene76529:CDS 50.0%
CACTGGCAGAAAAAGCAGCT+TGG + chr2.3:2701519-2701538 MS.gene76529:CDS 50.0%
CGAGTCATCTTGGAGCTCAT+TGG + chr2.3:2701256-2701275 MS.gene76529:CDS 50.0%
CTTTGCATCGTTTGTGGAGC+AGG + chr2.3:2702469-2702488 MS.gene76529:CDS 50.0%
GCCTAACACATCAGATGCAG+TGG - chr2.3:2701630-2701649 None:intergenic 50.0%
GGCAGAAATCTTGGAACCAG+CGG + chr2.3:2701109-2701128 MS.gene76529:intron 50.0%
TAGCTTCGACAACGCGATCA+TGG + chr2.3:2700869-2700888 MS.gene76529:CDS 50.0%
! CCTTTGGGAATGGGAAAACC+AGG - chr2.3:2701921-2701940 None:intergenic 50.0%
! GACAGGAGCTTCAGGCTTTT+TGG + chr2.3:2700600-2700619 MS.gene76529:CDS 50.0%
!! CCTGGTTTTCCCATTCCCAA+AGG + chr2.3:2701918-2701937 MS.gene76529:CDS 50.0%
ATAACACAGCACGGGGTTGG+TGG - chr2.3:2702511-2702530 None:intergenic 55.0%
ATCTTGGAACCAGCGGTCCA+AGG + chr2.3:2701116-2701135 MS.gene76529:CDS 55.0%
GTGTGTGTGACAGGAGCTTC+AGG + chr2.3:2700592-2700611 MS.gene76529:CDS 55.0%
! GTTGGTGGGGAAGAGTGAGA+TGG - chr2.3:2702496-2702515 None:intergenic 55.0%
! TAACACAGCACGGGGTTGGT+GGG - chr2.3:2702510-2702529 None:intergenic 55.0%
TTCAGCGTACCTTGGACCGC+TGG - chr2.3:2701128-2701147 None:intergenic 60.0%
!! AACACAGCACGGGGTTGGTG+GGG - chr2.3:2702509-2702528 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 2700580 2702535 2700580 ID=MS.gene76529
chr2.3 mRNA 2700580 2702535 2700580 ID=MS.gene76529.t1;Parent=MS.gene76529
chr2.3 exon 2700580 2700688 2700580 ID=MS.gene76529.t1.exon1;Parent=MS.gene76529.t1
chr2.3 CDS 2700580 2700688 2700580 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
chr2.3 exon 2700781 2700953 2700781 ID=MS.gene76529.t1.exon2;Parent=MS.gene76529.t1
chr2.3 CDS 2700781 2700953 2700781 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
chr2.3 exon 2701110 2701298 2701110 ID=MS.gene76529.t1.exon3;Parent=MS.gene76529.t1
chr2.3 CDS 2701110 2701298 2701110 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
chr2.3 exon 2701497 2701659 2701497 ID=MS.gene76529.t1.exon4;Parent=MS.gene76529.t1
chr2.3 CDS 2701497 2701659 2701497 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
chr2.3 exon 2701748 2701940 2701748 ID=MS.gene76529.t1.exon5;Parent=MS.gene76529.t1
chr2.3 CDS 2701748 2701940 2701748 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
chr2.3 exon 2702370 2702535 2702370 ID=MS.gene76529.t1.exon6;Parent=MS.gene76529.t1
chr2.3 CDS 2702370 2702535 2702370 ID=cds.MS.gene76529.t1;Parent=MS.gene76529.t1
Gene Sequence

>MS.gene76529

ATGGAACACAAAGTGTGTGTGACAGGAGCTTCAGGCTTTTTGGCTTCTTGGCTTATCAAGAGACTTCTTTTGTCTGGATATCATGTTATTGGAACAGTTAGAGATTTAGGTAAAATGTATTTTATGCTGACGAAATGTATTTTCACTTTTCACATTTAAATACATTTAACATATGTATGACATTTTTTTATACACTTGCAGGTAAGAAGCAGAAAGTTGAACATTTATGGAAGTTGGAAGGAGCAACAGAAAGACTCAAACTAGTCCAAGCTGATTTAATGGAAGAAAATAGCTTCGACAACGCGATCATGGGATGCAAAGGTGTCTTTCACATTGCCTCTCCGGTACTCAATCACATATCAAATGATCCTAAGGTGTGATATTTCATTTATGTTATTCATAGGTATCTCTATGTCACAACCTATAAGCTCATACATTAATCAAAATCTTCACATCATGGAAACTACACATAGTGAAATAAGTTATGATTAATTTCTAGTATGATTATCAATTTTTGTCCAAAAAAACAGGCAGAAATCTTGGAACCAGCGGTCCAAGGTACGCTGAATGTTTTGCGTTCTTGTCGAAAGAATCCAGCCCTTGTTCGAGTTGTGTTAGCCTCATCGTCTTCAGCTGTTAGAGTAAGAGCTGATTTCGATTCAAACATACCATTAGACGAGTCATCTTGGAGCTCATTGGAATTGTGTGAGAAACTCCAGGTACACATTTCCTAACTAATCATATATGTATAAGATTTTGATTAGCATGTACTCAATCTAAAAATGATTTATACATATATTACTTTTTAAATCATGTTCTCATGTGACAAGATCATGAAATGTATTTTGAGGTACATATACTTTTTTTTACAAATGGATAGTTATTAAACTTACGTGACAAACTTGTATTGAATTAAGGCATGGTACCCAATGTCAAAGACACTGGCAGAAAAAGCAGCTTGGGAATATTGCAAAGAGAATGGAATAGACTTGGTGACTGTTTTACCATCATTCATAATTGGACCTAACTTACCAACAGATTTATGTTCCACTGCATCTGATGTGTTAGGCTTGTTCAAAGGTACATGCACACAAATGTTCTAGGCTTAATTTGATTGATATATTTTGTAAAATTATATGATTAACCATATTGCTAATTAAACTAACAGGGCAAACTGAGAAATTTCAATGGCATGGAAGGATGGGATATGTTCATATCGATGATGTTGCACTTTGTCACATCCTTCTTTATGAAAACAAAGCCTCTCATGGTAGATACCTTTGCAGTTCAAAAATAATGGATAATGATGATTTGGTTGCCATGCTAGCAAATCGTTATCCTGGTTTTCCCATTCCCAAAGGGTTGGTTCATGCTTCTCATTACCTTAGCATAGTTAGTATCTATATACATTTGATAGAGTATCGCAGATTTAATTTGTATACATAGACACTATAAAAATAAAATAAAATAAAATAAAATACACATGCATCAAATCAAATTAGATCGCGTAGATATTATTTAGAAATAAACTTAAGAAAATTGTACTTAATACTATAATGTTATTTGATTGGATACATGTGTAAAAAAAAAAAATTACACCATCAATATAAAGAAATTAATCACATTACAATTGTATTTAATTCTATGATGTTATTTGATTGGATACATGTGTAAAAAAAAATTACACCATTGATATAAAGAAATTAATCACATCACAAATATTGGAAACTAGAAAATGTAAACACAAAAAACTAATGGGTATATCTTTGTATGATTGATTTGGTTTTCAGGTTCAAGAAACTAGATAGGCCACATTATGAACTAAACACTGGAAAGCTGGAGAGTTTAGGATTCAAGTTTAAATCTGTTGAAGAAATGTTTGATGATTGCTTTGCATCGTTTGTGGAGCAGGGCCATCTCACTCTTCCCCACCAACCCCGTGCTGTGTTATTGTAA
Protein sequence

>MS.gene76529.t1

MEHKVCVTGASGFLASWLIKRLLLSGYHVIGTVRDLGKKQKVEHLWKLEGATERLKLVQADLMEENSFDNAIMGCKGVFHIASPVLNHISNDPKAEILEPAVQGTLNVLRSCRKNPALVRVVLASSSSAVRVRADFDSNIPLDESSWSSLELCEKLQAWYPMSKTLAEKAAWEYCKENGIDLVTVLPSFIIGPNLPTDLCSTASDVLGLFKGQTEKFQWHGRMGYVHIDDVALCHILLYENKASHGRYLCSSKIMDNDDLVAMLANRYPGFPIPKGFKKLDRPHYELNTGKLESLGFKFKSVEEMFDDCFASFVEQGHLTLPHQPRAVLL