AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058751

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058751.t1 MTR_3g005170 89.815 324 8 1 2 300 3 326 0.0 586
MS.gene058751.t1 MTR_3g005210 83.438 320 28 1 6 300 3 322 0.0 540
MS.gene058751.t1 MTR_3g005170 80.247 324 8 2 2 300 3 295 0.0 502
MS.gene058751.t1 MTR_3g005170 88.448 277 7 1 2 253 3 279 1.08e-178 494
MS.gene058751.t1 MTR_8g062110 65.538 325 86 2 1 300 1 324 9.98e-154 433
MS.gene058751.t1 MTR_8g062110 65.538 325 86 2 1 300 10 333 1.42e-153 432
MS.gene058751.t1 MTR_8g062440 65.635 323 85 2 3 300 2 323 6.96e-152 428
MS.gene058751.t1 MTR_8g062440 65.432 324 85 2 3 300 2 324 1.05e-151 427
MS.gene058751.t1 MTR_4g077100 62.462 325 97 2 1 300 3 327 5.94e-151 426
MS.gene058751.t1 MTR_4g077130 60.923 325 102 1 1 300 1 325 2.76e-147 416
MS.gene058751.t1 MTR_8g062110 64.748 278 72 2 1 253 1 277 9.72e-126 360
MS.gene058751.t1 MTR_8g062110 64.748 278 72 2 1 253 1 277 1.08e-125 360
MS.gene058751.t1 MTR_4g077100 61.871 278 81 2 1 253 3 280 9.93e-125 357
MS.gene058751.t1 MTR_8g062440 59.615 312 92 3 3 281 2 312 3.04e-122 353
MS.gene058751.t1 MTR_8g062110 70.306 229 67 1 72 300 11 238 7.63e-119 341
MS.gene058751.t1 MTR_8g062110 70.330 182 53 1 72 253 11 191 6.23e-91 268
MS.gene058751.t1 MTR_0685s0030 64.024 164 59 0 137 300 1 164 7.88e-77 231
MS.gene058751.t1 MTR_5g072620 42.105 323 156 6 5 299 1 320 1.69e-76 236
MS.gene058751.t1 MTR_4g006940 47.518 282 139 6 16 295 49 323 1.14e-74 232
MS.gene058751.t1 MTR_2g104960 42.331 326 154 7 2 300 13 331 5.38e-70 220
MS.gene058751.t1 MTR_5g056600 40.138 289 162 7 16 298 36 319 1.93e-69 218
MS.gene058751.t1 MTR_2g101330 41.281 281 157 6 19 295 40 316 5.59e-69 218
MS.gene058751.t1 MTR_3g031650 37.578 322 170 6 5 298 1 319 1.13e-67 214
MS.gene058751.t1 MTR_1g022440 34.568 324 178 6 3 295 2 322 1.13e-60 196
MS.gene058751.t1 MTR_4g006940 47.280 239 118 5 16 253 49 280 1.36e-60 194
MS.gene058751.t1 MTR_4g006940 47.280 239 118 5 16 253 49 280 1.36e-60 194
MS.gene058751.t1 MTR_4g009040 39.446 289 164 7 11 294 37 319 1.18e-59 193
MS.gene058751.t1 MTR_3g031650 38.519 270 135 6 5 246 1 267 3.85e-59 190
MS.gene058751.t1 MTR_4g009040 39.249 293 163 8 11 294 37 323 5.12e-58 189
MS.gene058751.t1 MTR_2g028620 36.556 331 171 9 2 299 3 327 3.33e-57 187
MS.gene058751.t1 MTR_1g022445 33.934 333 178 9 3 298 2 329 4.38e-57 187
MS.gene058751.t1 MTR_3g011130 79.310 116 23 1 75 189 30 145 1.44e-56 179
MS.gene058751.t1 MTR_4g009690 39.583 288 164 6 11 294 37 318 8.64e-53 176
MS.gene058751.t1 MTR_4g009690 39.446 289 164 7 11 294 37 319 3.40e-51 171
MS.gene058751.t1 MTR_0685s0030 63.248 117 43 0 137 253 1 117 9.81e-51 163
MS.gene058751.t1 MTR_2g028620 38.989 277 130 9 2 245 3 273 3.84e-49 166
MS.gene058751.t1 MTR_4g092080 36.177 293 171 7 16 295 46 335 1.37e-48 165
MS.gene058751.t1 MTR_4g077150 47.458 177 41 4 74 250 21 145 3.88e-46 153
MS.gene058751.t1 MTR_4g081440 34.459 296 166 8 17 289 50 340 1.57e-43 152
MS.gene058751.t1 MTR_7g074820 33.448 290 179 6 19 298 44 329 2.59e-43 151
MS.gene058751.t1 MTR_4g081440 35.135 296 165 9 17 289 54 345 2.88e-43 152
MS.gene058751.t1 MTR_7g074880 31.650 297 181 8 16 298 38 326 3.00e-42 148
MS.gene058751.t1 MTR_7g074850 31.615 291 183 7 19 298 44 329 1.50e-39 141
MS.gene058751.t1 MTR_7g074730 31.488 289 185 6 19 298 44 328 1.91e-39 141
MS.gene058751.t1 MTR_7g074870 30.584 291 191 7 15 298 2 288 1.22e-38 138
MS.gene058751.t1 MTR_7g074710 35.270 241 146 5 17 250 39 276 9.25e-38 136
MS.gene058751.t1 MTR_1g099230 93.548 62 4 0 75 136 36 97 2.71e-34 123
MS.gene058751.t1 MTR_4g023730 31.818 242 151 7 17 250 43 278 1.12e-29 115
MS.gene058751.t1 MTR_6g406250 27.972 286 175 11 16 284 84 355 3.43e-26 106
MS.gene058751.t1 MTR_0164s0070 53.333 90 41 1 20 108 41 130 1.14e-24 99.0
MS.gene058751.t1 MTR_5g029990 24.506 253 173 6 32 278 56 296 3.15e-22 94.4
MS.gene058751.t1 MTR_6g406250 30.769 169 98 5 16 172 84 245 7.51e-21 90.1
MS.gene058751.t1 MTR_2g013250 27.344 256 167 5 17 253 40 295 6.18e-18 83.2
MS.gene058751.t1 MTR_2g013230 26.154 260 165 5 17 253 41 296 8.03e-16 77.0
MS.gene058751.t1 MTR_2g013260 26.953 256 168 5 17 253 41 296 1.29e-15 75.9
MS.gene058751.t1 MTR_2g013260 26.953 256 168 5 17 253 41 296 2.88e-15 75.5
MS.gene058751.t1 MTR_7g117190 26.894 264 165 9 17 256 40 299 1.56e-14 73.2
MS.gene058751.t1 MTR_7g117190 26.523 279 172 10 17 271 40 309 5.47e-14 71.6
MS.gene058751.t1 MTR_8g062490 51.562 64 31 0 191 254 54 117 9.71e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058751.t1 AT5G19440 69.136 324 75 1 2 300 3 326 9.31e-167 466
MS.gene058751.t1 AT1G51410 68.000 325 79 1 1 300 1 325 2.20e-166 465
MS.gene058751.t1 AT1G09480 46.984 315 142 1 6 295 5 319 4.89e-98 291
MS.gene058751.t1 AT1G09480 46.984 315 142 1 6 295 52 366 1.03e-97 292
MS.gene058751.t1 AT1G09510 46.541 318 145 1 6 298 5 322 1.14e-97 290
MS.gene058751.t1 AT1G09490 49.117 283 144 0 13 295 6 288 1.38e-95 284
MS.gene058751.t1 AT1G09490 46.667 315 143 1 6 295 5 319 2.98e-95 284
MS.gene058751.t1 AT1G66800 46.984 315 138 2 6 295 5 315 2.35e-90 272
MS.gene058751.t1 AT1G09500 45.483 321 147 4 6 298 5 325 8.08e-89 268
MS.gene058751.t1 AT1G09500 47.902 286 146 3 16 298 6 291 4.93e-88 265
MS.gene058751.t1 AT1G09500 45.985 274 122 2 6 253 5 278 3.56e-79 242
MS.gene058751.t1 AT4G35420 43.197 294 159 5 11 299 32 322 1.27e-77 239
MS.gene058751.t1 AT5G58490 41.590 327 160 6 1 299 1 324 1.55e-75 234
MS.gene058751.t1 AT1G15950 42.236 322 152 7 1 295 5 319 1.05e-69 220
MS.gene058751.t1 AT1G15950 42.236 322 152 7 1 295 5 319 1.05e-69 220
MS.gene058751.t1 AT1G15950 41.587 315 150 7 1 288 5 312 5.07e-66 210
MS.gene058751.t1 AT2G02400 39.785 279 162 5 23 298 43 318 8.26e-65 206
MS.gene058751.t1 AT1G80820 40.373 322 158 8 6 300 5 319 3.11e-64 205
MS.gene058751.t1 AT1G68540 40.226 266 149 5 17 276 37 298 5.53e-63 202
MS.gene058751.t1 AT1G15950 41.935 279 129 6 1 253 5 276 9.01e-58 187
MS.gene058751.t1 AT1G15950 41.463 287 134 7 1 261 5 283 1.43e-57 187
MS.gene058751.t1 AT1G25460 36.395 294 183 4 5 295 24 316 4.27e-57 186
MS.gene058751.t1 AT1G15950 41.877 277 128 6 1 251 5 274 6.18e-57 185
MS.gene058751.t1 AT2G33590 38.776 294 170 6 11 299 33 321 1.40e-55 182
MS.gene058751.t1 AT1G15950 43.946 223 121 4 75 295 1 221 3.34e-51 169
MS.gene058751.t1 AT1G80820 40.146 274 131 7 6 253 5 271 8.89e-51 169
MS.gene058751.t1 AT2G33600 38.983 295 168 7 11 299 33 321 1.66e-50 169
MS.gene058751.t1 AT5G42800 32.919 322 182 7 8 298 7 325 2.23e-48 166
MS.gene058751.t1 AT1G76470 38.488 291 167 9 11 293 31 317 2.30e-46 159
MS.gene058751.t1 AT1G61720 32.990 291 177 6 16 294 45 329 5.87e-43 150
MS.gene058751.t1 AT1G68540 37.500 192 117 3 87 276 1 191 1.65e-39 138
MS.gene058751.t1 AT4G27250 32.042 284 175 8 32 300 58 338 4.00e-39 140
MS.gene058751.t1 AT4G27250 31.741 293 174 9 32 300 58 348 9.66e-39 140
MS.gene058751.t1 AT2G23910 28.947 228 151 6 27 251 19 238 5.98e-32 120
MS.gene058751.t1 AT2G23910 28.696 230 153 6 27 253 53 274 8.87e-32 119
MS.gene058751.t1 AT2G23910 28.947 228 151 6 27 251 53 272 1.38e-31 119
MS.gene058751.t1 AT2G23910 28.947 228 151 6 27 251 53 272 1.54e-31 119
MS.gene058751.t1 AT4G30470 28.992 238 157 6 31 265 57 285 3.90e-31 118
MS.gene058751.t1 AT2G45400 28.832 274 188 2 12 278 70 343 8.03e-31 119
MS.gene058751.t1 AT2G45400 28.996 269 184 2 17 278 18 286 1.23e-30 117
MS.gene058751.t1 AT2G45400 29.555 247 167 2 12 251 70 316 7.03e-30 115
MS.gene058751.t1 AT2G45400 30.270 185 124 1 12 191 70 254 1.90e-22 94.4
MS.gene058751.t1 AT5G14700 28.311 219 139 8 46 256 124 332 1.06e-21 94.4
MS.gene058751.t1 AT5G14700 32.000 150 90 5 46 191 124 265 1.92e-16 78.2

Find 71 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCATCCCAAACTAGAATT+TGG 0.171474 3.1:-551593 None:intergenic
GACATTTCCAAATTCTAGTT+TGG 0.253982 3.1:+551586 MS.gene058751:CDS
AACCAAGTGGTCAACCTTCT+TGG 0.257463 3.1:-550749 None:intergenic
CATTCTCGTACGCCAGAATA+TGG 0.258064 3.1:-551640 None:intergenic
ATTGCTGCTGTTGCGTATAA+TGG 0.272149 3.1:+551009 MS.gene058751:CDS
GCAATTCTAAACCTGATTAA+TGG 0.275531 3.1:+551329 MS.gene058751:CDS
AATTATTAATCTTACTTCTC+TGG 0.283324 3.1:-551758 None:intergenic
TCTTTGGAAACCTGATATAT+AGG 0.308566 3.1:-552950 None:intergenic
TAAAGAAAACAACATTGATA+TGG 0.308617 3.1:+551241 MS.gene058751:CDS
TTGCAGCAGCACTTGTGTTT+AGG 0.321177 3.1:-551310 None:intergenic
TGTTGCAAATGCCCATATTC+TGG 0.323215 3.1:+551628 MS.gene058751:CDS
GTAATTAATTACCATTAATC+AGG 0.328588 3.1:-551340 None:intergenic
ATTAACCCAGCAATGGTCAT+AGG 0.359026 3.1:+551269 MS.gene058751:CDS
GCCTTTGATTCTGTTGTTCA+AGG 0.362009 3.1:+550832 MS.gene058751:CDS
GTCCTTGACATCATGATAGA+AGG 0.366719 3.1:-550884 None:intergenic
GTATGTGTGACTGGCGCTTC+AGG 0.374333 3.1:+550364 MS.gene058751:CDS
ACCAAGTGGTCAACCTTCTT+GGG 0.387248 3.1:-550748 None:intergenic
ACATTTCCAAATTCTAGTTT+GGG 0.393375 3.1:+551587 MS.gene058751:CDS
AGAGGCCCTATGACCATTGC+TGG 0.404733 3.1:-551275 None:intergenic
ATTATTAATCTTACTTCTCT+GGG 0.410205 3.1:-551757 None:intergenic
GGAGAGATTGCAACTGTTCA+AGG 0.418796 3.1:+550789 MS.gene058751:CDS
GCCGTATGTGCCTATATATC+AGG 0.426554 3.1:+552940 MS.gene058751:CDS
GGGACTTGAGTATACTCCTT+TGG 0.434471 3.1:+552988 MS.gene058751:CDS
GACCACTTGGTTAAGCTTGA+AGG 0.439383 3.1:+550760 MS.gene058751:CDS
CAAAGAAAAGGCGAAAAGCT+TGG 0.447824 3.1:+552967 MS.gene058751:CDS
TCCCAAGAAGGTTGACCACT+TGG 0.453559 3.1:+550747 MS.gene058751:CDS
GAGAAAATGAGCAGCAACTT+GGG 0.454229 3.1:+550334 None:intergenic
AGACAAGACGTTTGAGTGAT+GGG 0.455678 3.1:-550975 None:intergenic
AAAGAAAAGGCGAAAAGCTT+GGG 0.461360 3.1:+552968 MS.gene058751:CDS
AGAGAAAATGAGCAGCAACT+TGG 0.464708 3.1:+550333 None:intergenic
TCCAAATTCTAGTTTGGGAT+GGG 0.474083 3.1:+551592 MS.gene058751:CDS
TTCCAAATTCTAGTTTGGGA+TGG 0.486809 3.1:+551591 MS.gene058751:CDS
ATATCAGGTTTCCAAAGAAA+AGG 0.488979 3.1:+552955 MS.gene058751:CDS
TTGAGAGTCCCTTTGAGAGC+AGG 0.491416 3.1:-550928 None:intergenic
ATTGTTTCAGCTGTGGTATG+TGG 0.496971 3.1:+551178 MS.gene058751:intron
ATTCTCGTACGCCAGAATAT+GGG 0.502026 3.1:-551639 None:intergenic
TTGATTGATCCTGCTCTCAA+AGG 0.509724 3.1:+550919 MS.gene058751:CDS
TTTAGGACTGGTTGCAAGAG+AGG 0.512693 3.1:-551293 None:intergenic
ACCTGATATATAGGCACATA+CGG 0.513032 3.1:-552941 None:intergenic
TGATTGATCCTGCTCTCAAA+GGG 0.514573 3.1:+550920 MS.gene058751:CDS
TCATGATGTCAAGGACCCTC+AGG 0.517650 3.1:+550891 MS.gene058751:CDS
GCAGCACTTGTGTTTAGGAC+TGG 0.518349 3.1:-551305 None:intergenic
AAGACAAGACGTTTGAGTGA+TGG 0.518377 3.1:-550976 None:intergenic
TCTGTTGTTCAAGGTTGTCA+TGG 0.518898 3.1:+550841 MS.gene058751:CDS
TACGAGAATGCTTCAGCTAG+TGG 0.527796 3.1:+551653 MS.gene058751:CDS
GTCTCATTGACAACAACATC+AGG 0.531435 3.1:-551045 None:intergenic
GGTTAAGCTTGAAGGTGCTA+AGG 0.535332 3.1:+550768 MS.gene058751:CDS
TCCTTGATGCTCACTTCCAA+AGG 0.541588 3.1:-553004 None:intergenic
TTAACCCAGCAATGGTCATA+GGG 0.542832 3.1:+551270 MS.gene058751:CDS
CAAGGCAAATCTACTTGAAG+AGG 0.550142 3.1:+550807 MS.gene058751:CDS
GGAAATGTTGTATGTGTGAC+TGG 0.550828 3.1:+550355 MS.gene058751:CDS
GACAAGACGTTTGAGTGATG+GGG 0.560252 3.1:-550974 None:intergenic
TCCCTTCTATCATGATGTCA+AGG 0.565747 3.1:+550882 MS.gene058751:CDS
ACCTTGAACAACAGAATCAA+AGG 0.567407 3.1:-550833 None:intergenic
ATGTTGTTGTCAATGAGACT+TGG 0.575014 3.1:+551049 MS.gene058751:CDS
ACAACAACATCAGGAGTTCG+AGG 0.578908 3.1:-551036 None:intergenic
CATTGGCTGAAGAAGCTGCA+TGG 0.580278 3.1:+551210 MS.gene058751:CDS
TCCTTGACATCATGATAGAA+GGG 0.582694 3.1:-550883 None:intergenic
TTCTCTGGGAGTTGCAACGA+AGG 0.598543 3.1:-551743 None:intergenic
GTATGTGGTTTCAAAGACAT+TGG 0.606075 3.1:+551193 MS.gene058751:CDS
CACCTTCAAGCTTAACCAAG+TGG 0.608434 3.1:-550762 None:intergenic
GCCTGTAGATGATCCCAAGA+AGG 0.612585 3.1:+550735 MS.gene058751:intron
TCCTTTGGAAGTGAGCATCA+AGG 0.622590 3.1:+553003 MS.gene058751:CDS
CACACTACTCAGAAGTTGTG+AGG 0.628161 3.1:+551702 MS.gene058751:CDS
GAGGCCCTATGACCATTGCT+GGG 0.631385 3.1:-551274 None:intergenic
GGATCAATCAATTCAGCCTG+AGG 0.632382 3.1:-550907 None:intergenic
GGTTACTATTAACCCAGCAA+TGG 0.635018 3.1:+551262 MS.gene058751:CDS
TCTCTGGGAGTTGCAACGAA+GGG 0.653512 3.1:-551742 None:intergenic
AAGGCAAATCTACTTGAAGA+GGG 0.656212 3.1:+550808 MS.gene058751:CDS
AACTATGTTTACCAGATGTG+CGG 0.691679 3.1:+552910 MS.gene058751:intron
GATCAATCAATTCAGCCTGA+GGG 0.692249 3.1:-550906 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATGTAAATACAGAAA+CGG - chr3.1:552827-552846 None:intergenic 15.0%
!!! ATTAAGTGTTTCTAAAAATA+CGG + chr3.1:552700-552719 MS.gene058751:intron 15.0%
!! AATAGAATCTCTCAAAATAA+GGG + chr3.1:552056-552075 MS.gene058751:intron 20.0%
!! AATTATTAATCTTACTTCTC+TGG - chr3.1:551761-551780 None:intergenic 20.0%
!! ACTATCATATAGTTAAAGTA+TGG + chr3.1:552259-552278 MS.gene058751:intron 20.0%
!! ATTATTAATCTTACTTCTCT+GGG - chr3.1:551760-551779 None:intergenic 20.0%
!! CAATTTAATGCTAAAATGAA+AGG + chr3.1:551882-551901 MS.gene058751:intron 20.0%
!! CTATCATATAGTTAAAGTAT+GGG + chr3.1:552260-552279 MS.gene058751:intron 20.0%
!! GTAATTAATTACCATTAATC+AGG - chr3.1:551343-551362 None:intergenic 20.0%
!! TAAAGAAAACAACATTGATA+TGG + chr3.1:551241-551260 MS.gene058751:CDS 20.0%
!! TTTACATAACAAGATTGTAA+AGG + chr3.1:552024-552043 MS.gene058751:intron 20.0%
!!! AAATAAAGCTTTAAAGAATG+TGG - chr3.1:551848-551867 None:intergenic 20.0%
!!! GTTAAAAAGTACAGAAATAA+TGG - chr3.1:552467-552486 None:intergenic 20.0%
!!! TGTACTGAATTTTTCATTTT+GGG + chr3.1:552665-552684 MS.gene058751:intron 20.0%
!!! TTGTACTGAATTTTTCATTT+TGG + chr3.1:552664-552683 MS.gene058751:intron 20.0%
! ACCAATAATAATTTCAATCC+GGG - chr3.1:550615-550634 None:intergenic 25.0%
! ATAACACCTTCTATCATATT+GGG + chr3.1:552561-552580 MS.gene058751:intron 25.0%
! ATTGAAATTATTATTGGTGG+TGG + chr3.1:550617-550636 MS.gene058751:intron 25.0%
! CAATAGAATCTCTCAAAATA+AGG + chr3.1:552055-552074 MS.gene058751:intron 25.0%
! CACCAATAATAATTTCAATC+CGG - chr3.1:550616-550635 None:intergenic 25.0%
! GAAAGAAAAAGACAAATACT+TGG - chr3.1:550460-550479 None:intergenic 25.0%
! GAGTATAAAACAACATTTCT+AGG - chr3.1:552747-552766 None:intergenic 25.0%
! TATAACACCTTCTATCATAT+TGG + chr3.1:552560-552579 MS.gene058751:intron 25.0%
! TCTTTAATGTATACGGAAAA+AGG + chr3.1:551940-551959 MS.gene058751:intron 25.0%
!! AATAAGGGAAAGTACATTAA+TGG + chr3.1:552071-552090 MS.gene058751:intron 25.0%
!! GTTTTATACTCGAAAATCAT+AGG + chr3.1:552756-552775 MS.gene058751:intron 25.0%
!!! ACATTTCCAAATTCTAGTTT+GGG + chr3.1:551587-551606 MS.gene058751:CDS 25.0%
!!! GGTTGTTTCTTTAATGTATA+CGG + chr3.1:551933-551952 MS.gene058751:intron 25.0%
AACATGAGCTATAAATCGAA+AGG - chr3.1:552419-552438 None:intergenic 30.0%
AAGTTACCCAATATGATAGA+AGG - chr3.1:552570-552589 None:intergenic 30.0%
ACAGAATGATGAATCATGTA+GGG - chr3.1:552804-552823 None:intergenic 30.0%
ACCCGGATTGAAATTATTAT+TGG + chr3.1:550611-550630 MS.gene058751:intron 30.0%
ACTCGAAAATCATAGGTTAA+TGG + chr3.1:552763-552782 MS.gene058751:intron 30.0%
AGCTTCTATTATCTACAGAA+GGG - chr3.1:551539-551558 None:intergenic 30.0%
ATAGAGAACATATCAACTAG+GGG - chr3.1:552236-552255 None:intergenic 30.0%
ATATCAGGTTTCCAAAGAAA+AGG + chr3.1:552955-552974 MS.gene058751:CDS 30.0%
ATTGATTATACAGACCAAGT+TGG - chr3.1:552169-552188 None:intergenic 30.0%
CAAATATGTTTGTGTTCAAG+AGG + chr3.1:551502-551521 MS.gene058751:intron 30.0%
CGGATTGAAATTATTATTGG+TGG + chr3.1:550614-550633 MS.gene058751:intron 30.0%
GATAGAGAACATATCAACTA+GGG - chr3.1:552237-552256 None:intergenic 30.0%
GCAATTCTAAACCTGATTAA+TGG + chr3.1:551329-551348 MS.gene058751:CDS 30.0%
TAACTATAATCACCTTCTAG+CGG + chr3.1:552122-552141 MS.gene058751:intron 30.0%
TACAGAAGACACAAAAATAG+AGG - chr3.1:552617-552636 None:intergenic 30.0%
TATATTCTATGCATCCAACT+TGG + chr3.1:552152-552171 MS.gene058751:intron 30.0%
TGATAGAGAACATATCAACT+AGG - chr3.1:552238-552257 None:intergenic 30.0%
! AATATGTATGTGTCCTGTTT+TGG + chr3.1:550641-550660 MS.gene058751:intron 30.0%
! CAATCTTGTTATGTAAAGCA+AGG - chr3.1:552021-552040 None:intergenic 30.0%
! CATTGCTAATAGTTGATTCA+GGG + chr3.1:551912-551931 MS.gene058751:intron 30.0%
! GACATTTCCAAATTCTAGTT+TGG + chr3.1:551586-551605 MS.gene058751:CDS 30.0%
! TCATTGCTAATAGTTGATTC+AGG + chr3.1:551911-551930 MS.gene058751:intron 30.0%
! TCTTTGGAAACCTGATATAT+AGG - chr3.1:552953-552972 None:intergenic 30.0%
!! AAAACAACATTTCTAGGACT+TGG - chr3.1:552741-552760 None:intergenic 30.0%
!!! AATTCAAAAGCGTTTTTGAG+AGG - chr3.1:551119-551138 None:intergenic 30.0%
AAAGAAAAGGCGAAAAGCTT+GGG + chr3.1:552968-552987 MS.gene058751:CDS 35.0%
AACTATAATCACCTTCTAGC+GGG + chr3.1:552123-552142 MS.gene058751:intron 35.0%
AACTATGTTTACCAGATGTG+CGG + chr3.1:552910-552929 MS.gene058751:intron 35.0%
AAGCAACAATGAAGCAATTG+GGG - chr3.1:551445-551464 None:intergenic 35.0%
AAGGCAAATCTACTTGAAGA+GGG + chr3.1:550808-550827 MS.gene058751:CDS 35.0%
ACAAGCAACAATGAAGCAAT+TGG - chr3.1:551447-551466 None:intergenic 35.0%
ACCTGATATATAGGCACATA+CGG - chr3.1:552944-552963 None:intergenic 35.0%
ACCTTGAACAACAGAATCAA+AGG - chr3.1:550836-550855 None:intergenic 35.0%
ATGTTGTTGTCAATGAGACT+TGG + chr3.1:551049-551068 MS.gene058751:CDS 35.0%
CAAGCAACAATGAAGCAATT+GGG - chr3.1:551446-551465 None:intergenic 35.0%
CACATCTATGTGGATGAATT+GGG + chr3.1:552348-552367 MS.gene058751:intron 35.0%
GAAGACACAAAAATAGAGGA+AGG - chr3.1:552613-552632 None:intergenic 35.0%
GACAGAATGATGAATCATGT+AGG - chr3.1:552805-552824 None:intergenic 35.0%
GAGCTTCTATTATCTACAGA+AGG - chr3.1:551540-551559 None:intergenic 35.0%
GTATGTGGTTTCAAAGACAT+TGG + chr3.1:551193-551212 MS.gene058751:CDS 35.0%
TCCTTGACATCATGATAGAA+GGG - chr3.1:550886-550905 None:intergenic 35.0%
TTTAACACATATTCAGCCTC+TGG + chr3.1:552481-552500 MS.gene058751:intron 35.0%
!! TCCAAATTCTAGTTTGGGAT+GGG + chr3.1:551592-551611 MS.gene058751:CDS 35.0%
!! TTCCAAATTCTAGTTTGGGA+TGG + chr3.1:551591-551610 MS.gene058751:CDS 35.0%
AAACCATGTGTTACGTACTG+GGG - chr3.1:552508-552527 None:intergenic 40.0%
AAGACAAGACGTTTGAGTGA+TGG - chr3.1:550979-550998 None:intergenic 40.0%
ACAAACACCAACACCAAAAC+AGG - chr3.1:550657-550676 None:intergenic 40.0%
ACAAATACTTGGATCGCGAA+CGG - chr3.1:550449-550468 None:intergenic 40.0%
ACCCATCCCAAACTAGAATT+TGG - chr3.1:551596-551615 None:intergenic 40.0%
AGACAAGACGTTTGAGTGAT+GGG - chr3.1:550978-550997 None:intergenic 40.0%
AGCAACAATGAAGCAATTGG+GGG - chr3.1:551444-551463 None:intergenic 40.0%
AGCCACGATTAAGAAGCAAT+CGG - chr3.1:550411-550430 None:intergenic 40.0%
ATAATTTCAATCCGGGTGTC+TGG - chr3.1:550608-550627 None:intergenic 40.0%
ATATATATGCTCCCGCTAGA+AGG - chr3.1:552137-552156 None:intergenic 40.0%
ATCTATGTGGATGAATTGGG+AGG + chr3.1:552351-552370 MS.gene058751:intron 40.0%
ATTAACCCAGCAATGGTCAT+AGG + chr3.1:551269-551288 MS.gene058751:CDS 40.0%
ATTCTCGTACGCCAGAATAT+GGG - chr3.1:551642-551661 None:intergenic 40.0%
ATTGCTGCTGTTGCGTATAA+TGG + chr3.1:551009-551028 MS.gene058751:CDS 40.0%
ATTGTTTCAGCTGTGGTATG+TGG + chr3.1:551178-551197 MS.gene058751:intron 40.0%
CAAAGAAAAGGCGAAAAGCT+TGG + chr3.1:552967-552986 MS.gene058751:CDS 40.0%
CAAGGCAAATCTACTTGAAG+AGG + chr3.1:550807-550826 MS.gene058751:CDS 40.0%
CCACAGCTGAAACAATCAAT+AGG - chr3.1:551174-551193 None:intergenic 40.0%
CTCTTTCACTTCATTGATGC+AGG + chr3.1:551557-551576 MS.gene058751:intron 40.0%
GAAACCATGTGTTACGTACT+GGG - chr3.1:552509-552528 None:intergenic 40.0%
GATCAATCAATTCAGCCTGA+GGG - chr3.1:550909-550928 None:intergenic 40.0%
GCACATCTATGTGGATGAAT+TGG + chr3.1:552347-552366 MS.gene058751:intron 40.0%
GCCTTTGATTCTGTTGTTCA+AGG + chr3.1:550832-550851 MS.gene058751:CDS 40.0%
GGAAATGTTGTATGTGTGAC+TGG + chr3.1:550355-550374 MS.gene058751:CDS 40.0%
GGACTTGGCACATTTATACT+TGG - chr3.1:552726-552745 None:intergenic 40.0%
GGTTACTATTAACCCAGCAA+TGG + chr3.1:551262-551281 MS.gene058751:CDS 40.0%
GTCCTTGACATCATGATAGA+AGG - chr3.1:550887-550906 None:intergenic 40.0%
GTCTCATTGACAACAACATC+AGG - chr3.1:551048-551067 None:intergenic 40.0%
TCAATTCGTCCACAAACACT+TGG - chr3.1:552288-552307 None:intergenic 40.0%
TCCCTTCTATCATGATGTCA+AGG + chr3.1:550882-550901 MS.gene058751:CDS 40.0%
TCTGTTGTTCAAGGTTGTCA+TGG + chr3.1:550841-550860 MS.gene058751:CDS 40.0%
TGAAACCATGTGTTACGTAC+TGG - chr3.1:552510-552529 None:intergenic 40.0%
TGATTGATCCTGCTCTCAAA+GGG + chr3.1:550920-550939 MS.gene058751:CDS 40.0%
TGCATTCACCAAACCTCTAT+GGG - chr3.1:552200-552219 None:intergenic 40.0%
TGTTGCAAATGCCCATATTC+TGG + chr3.1:551628-551647 MS.gene058751:CDS 40.0%
TTAACCCAGCAATGGTCATA+GGG + chr3.1:551270-551289 MS.gene058751:CDS 40.0%
TTGATTGATCCTGCTCTCAA+AGG + chr3.1:550919-550938 MS.gene058751:CDS 40.0%
TTGCATTCACCAAACCTCTA+TGG - chr3.1:552201-552220 None:intergenic 40.0%
! CAAGCTTTTCGCCTTTTCTT+TGG - chr3.1:552969-552988 None:intergenic 40.0%
! CCTATTGATTGTTTCAGCTG+TGG + chr3.1:551171-551190 MS.gene058751:intron 40.0%
!!! TATGTGTCCTGTTTTGGTGT+TGG + chr3.1:550647-550666 MS.gene058751:intron 40.0%
AACCAAGTGGTCAACCTTCT+TGG - chr3.1:550752-550771 None:intergenic 45.0%
ACAACAACATCAGGAGTTCG+AGG - chr3.1:551039-551058 None:intergenic 45.0%
ACCAAGTGGTCAACCTTCTT+GGG - chr3.1:550751-550770 None:intergenic 45.0%
ACCTTCTTGGGATCATCTAC+AGG - chr3.1:550739-550758 None:intergenic 45.0%
CAATGAAGCAATTGGGGGAA+AGG - chr3.1:551439-551458 None:intergenic 45.0%
CACACTACTCAGAAGTTGTG+AGG + chr3.1:551702-551721 MS.gene058751:CDS 45.0%
CACCTTCAAGCTTAACCAAG+TGG - chr3.1:550765-550784 None:intergenic 45.0%
CATTCTCGTACGCCAGAATA+TGG - chr3.1:551643-551662 None:intergenic 45.0%
CGTAACACATGGTTTCACTG+AGG + chr3.1:552513-552532 MS.gene058751:intron 45.0%
GACAAGACGTTTGAGTGATG+GGG - chr3.1:550977-550996 None:intergenic 45.0%
GACCACTTGGTTAAGCTTGA+AGG + chr3.1:550760-550779 MS.gene058751:CDS 45.0%
GCATTCACCAAACCTCTATG+GGG - chr3.1:552199-552218 None:intergenic 45.0%
GCCGTATGTGCCTATATATC+AGG + chr3.1:552940-552959 MS.gene058751:CDS 45.0%
GGAGAGATTGCAACTGTTCA+AGG + chr3.1:550789-550808 MS.gene058751:CDS 45.0%
GGATCAATCAATTCAGCCTG+AGG - chr3.1:550910-550929 None:intergenic 45.0%
GGGACTTGAGTATACTCCTT+TGG + chr3.1:552988-553007 MS.gene058751:CDS 45.0%
GTATGGGTACCAAGTGTTTG+TGG + chr3.1:552276-552295 MS.gene058751:intron 45.0%
GTCCGATTGCTTCTTAATCG+TGG + chr3.1:550406-550425 MS.gene058751:intron 45.0%
TACCATACGAAGCACAGACA+CGG - chr3.1:550682-550701 None:intergenic 45.0%
TACGAGAATGCTTCAGCTAG+TGG + chr3.1:551653-551672 MS.gene058751:CDS 45.0%
TCAATCTGACATCCCCATAG+AGG + chr3.1:552184-552203 MS.gene058751:intron 45.0%
TCCTTGATGCTCACTTCCAA+AGG - chr3.1:553007-553026 None:intergenic 45.0%
TCGAAAGGTGATCAAGTCTG+AGG - chr3.1:552404-552423 None:intergenic 45.0%
TGTGACTGAGCACATCTATG+TGG + chr3.1:552338-552357 MS.gene058751:intron 45.0%
TTGCAGCAGCACTTGTGTTT+AGG - chr3.1:551313-551332 None:intergenic 45.0%
! CTTCAGGTTACATCGCTTCA+TGG + chr3.1:550380-550399 MS.gene058751:intron 45.0%
! TCCTTTGGAAGTGAGCATCA+AGG + chr3.1:553003-553022 MS.gene058751:CDS 45.0%
!! GGTTAAGCTTGAAGGTGCTA+AGG + chr3.1:550768-550787 MS.gene058751:CDS 45.0%
!! TTTAGGACTGGTTGCAAGAG+AGG - chr3.1:551296-551315 None:intergenic 45.0%
!! AAATAATAATTATTATTGAT+TGG - chr3.1:550535-550554 None:intergenic 5.0%
AATACTTGGATCGCGAACGG+TGG - chr3.1:550446-550465 None:intergenic 50.0%
CTGACATCCCCATAGAGGTT+TGG + chr3.1:552189-552208 MS.gene058751:intron 50.0%
GCCTGTAGATGATCCCAAGA+AGG + chr3.1:550735-550754 MS.gene058751:intron 50.0%
GGTGATCAAGTCTGAGGAAC+AGG - chr3.1:552398-552417 None:intergenic 50.0%
TCATGATGTCAAGGACCCTC+AGG + chr3.1:550891-550910 MS.gene058751:CDS 50.0%
TCCCAAGAAGGTTGACCACT+TGG + chr3.1:550747-550766 MS.gene058751:CDS 50.0%
TGAATGAGACACCAGACACC+CGG + chr3.1:550594-550613 MS.gene058751:intron 50.0%
! CATTGGCTGAAGAAGCTGCA+TGG + chr3.1:551210-551229 MS.gene058751:CDS 50.0%
! GCAGCACTTGTGTTTAGGAC+TGG - chr3.1:551308-551327 None:intergenic 50.0%
! TCTCTGGGAGTTGCAACGAA+GGG - chr3.1:551745-551764 None:intergenic 50.0%
! TTCTCTGGGAGTTGCAACGA+AGG - chr3.1:551746-551765 None:intergenic 50.0%
! TTGAGAGTCCCTTTGAGAGC+AGG - chr3.1:550931-550950 None:intergenic 50.0%
AGAGGCCCTATGACCATTGC+TGG - chr3.1:551278-551297 None:intergenic 55.0%
CGGCTTATCATCCGCACATC+TGG - chr3.1:552924-552943 None:intergenic 55.0%
GAGGCCCTATGACCATTGCT+GGG - chr3.1:551277-551296 None:intergenic 55.0%
GGACCCCAGTACGTAACACA+TGG + chr3.1:552502-552521 MS.gene058751:intron 55.0%
GTATGTGTGACTGGCGCTTC+AGG + chr3.1:550364-550383 MS.gene058751:CDS 55.0%
GTCCGTGTCTGTGCTTCGTA+TGG + chr3.1:550677-550696 MS.gene058751:intron 55.0%
TGTTACGTACTGGGGTCCAG+AGG - chr3.1:552500-552519 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 550340 553069 550340 ID=MS.gene058751
chr3.1 mRNA 550340 553069 550340 ID=MS.gene058751.t1;Parent=MS.gene058751
chr3.1 exon 550340 550385 550340 ID=MS.gene058751.t1.exon1;Parent=MS.gene058751.t1
chr3.1 CDS 550340 550385 550340 ID=cds.MS.gene058751.t1;Parent=MS.gene058751.t1
chr3.1 exon 550743 551104 550743 ID=MS.gene058751.t1.exon2;Parent=MS.gene058751.t1
chr3.1 CDS 550743 551104 550743 ID=cds.MS.gene058751.t1;Parent=MS.gene058751.t1
chr3.1 exon 551188 551350 551188 ID=MS.gene058751.t1.exon3;Parent=MS.gene058751.t1
chr3.1 CDS 551188 551350 551188 ID=cds.MS.gene058751.t1;Parent=MS.gene058751.t1
chr3.1 exon 551579 551765 551579 ID=MS.gene058751.t1.exon4;Parent=MS.gene058751.t1
chr3.1 CDS 551579 551765 551579 ID=cds.MS.gene058751.t1;Parent=MS.gene058751.t1
chr3.1 exon 552925 553069 552925 ID=MS.gene058751.t1.exon5;Parent=MS.gene058751.t1
chr3.1 CDS 552925 553069 552925 ID=cds.MS.gene058751.t1;Parent=MS.gene058751.t1
Gene Sequence

>MS.gene058751

ATGAGCAGCAACTTGGGAAATGTTGTATGTGTGACTGGCGCTTCAGGTTACATCGCTTCATGGCTCGTCCGATTGCTTCTTAATCGTGGCTACACTGTTAAAGCCACCGTTCGCGATCCAAGTATTTGTCTTTTTCTTTCATTTCACTTCTCTTCTCTTCTGATCTATCAATCACTCACTCACACATCAACACCAATCAATAATAATTATTATTTCAGAAAATTGTATAATTCAATGTTTTGTTTTCACATGTATGAATGAGACACCAGACACCCGGATTGAAATTATTATTGGTGGTGGTAATATGTATGTGTCCTGTTTTGGTGTTGGTGTTTGTGTCCGTGTCTGTGCTTCGTATGGTATATGTTTGATTTGATTTGATTATAATTGTGTCTGCCTGTAGATGATCCCAAGAAGGTTGACCACTTGGTTAAGCTTGAAGGTGCTAAGGAGAGATTGCAACTGTTCAAGGCAAATCTACTTGAAGAGGGTGCCTTTGATTCTGTTGTTCAAGGTTGTCATGGTGTCTTTCACACTGCATCTCCCTTCTATCATGATGTCAAGGACCCTCAGGCTGAATTGATTGATCCTGCTCTCAAAGGGACTCTCAATGTTCTCAACTCATGTGCTAAATCCCCATCACTCAAACGTCTTGTCTTAACATCTTCTATTGCTGCTGTTGCGTATAATGGAAAACCTCGAACTCCTGATGTTGTTGTCAATGAGACTTGGTTTACTGATGCTGATTTATGTGCTAAATCAAACGTATGTGTGTGCCTCTCAAAAACGCTTTTGAATTATGCTATCATGATCTTTGACTTCTTTCTATTTCCTATTGATTGTTTCAGCTGTGGTATGTGGTTTCAAAGACATTGGCTGAAGAAGCTGCATGGAAATTTGTTAAAGAAAACAACATTGATATGGTTACTATTAACCCAGCAATGGTCATAGGGCCTCTCTTGCAACCAGTCCTAAACACAAGTGCTGCTGCAATTCTAAACCTGATTAATGGTAATTAATTACAATTCTAAACGCAGCAAATACTCTTATTATCTTTCTGTGTCTACATCTTGCATATTATTTTTCTTGTCAGCTACCTTTCCCCCAATTGCTTCATTGTTGCTTGTTGTGTTTAATGAATATTAGCAGTTCTTATGTACTTCAAATATGTTTGTGTTCAAGAGGACAAGATTTTGCCCTTCTGTAGATAATAGAAGCTCTTTCACTTCATTGATGCAGGTGCACAGACATTTCCAAATTCTAGTTTGGGATGGGTCAATGTGAAAGATGTTGCAAATGCCCATATTCTGGCGTACGAGAATGCTTCAGCTAGTGGAAGACATTGTTTAGTTGAGAGAGTAGCACACTACTCAGAAGTTGTGAGGATTTTACGTGAACTGTACCCTTCGTTGCAACTCCCAGAGAAGTAAGATTAATAATTTCGATAACTGAAAGATTGCATATAATTTATGTTCTTATATGAATGCTAATTCTCATCTGATGTGCCACATTCTTTAAAGCTTTATTTACTTGTTTGAAACTCAATTTAATGCTAAAATGAAAGGATTTAGTCATTGCTAATAGTTGATTCAGGGTTGTTTCTTTAATGTATACGGAAAAAGGTAACTAAGTGAAAAATGCAGAAAAATTTCAACAATTGAATGTTTTAGAATAAATGCCTTGCTTTACATAACAAGATTGTAAAGGTTTATATACAATAGAATCTCTCAAAATAAGGGAAAGTACATTAATGGTAATAAACAAAGAGATGAATTCTAATTGTAACTATAATCACCTTCTAGCGGGAGCATATATATTCTATGCATCCAACTTGGTCTGTATAATCAATCTGACATCCCCATAGAGGTTTGGTGAATGCAACTTCATATTTGTCCCCTAGTTGATATGTTCTCTATCAGACTATCATATAGTTAAAGTATGGGTACCAAGTGTTTGTGGACGAATTGATTCATTTGAGTTGTTTTCTATGTGCTTGCTTGTGACTGAGCACATCTATGTGGATGAATTGGGAGGTTGCTTTATGTTATCTGATGACCTGTTCCTCAGACTTGATCACCTTTCGATTTATAGCTCATGTTAATTAGCTTTACTTCAGTATAACTACCATTATTTCTGTACTTTTTAACACATATTCAGCCTCTGGACCCCAGTACGTAACACATGGTTTCACTGAGGTTTTACTAATTTCTTTTTCAATAATATAACACCTTCTATCATATTGGGTAACTTGAATGTCATGATGACTTGCACCTTCCTCTATTTTTGTGTCTTCTGTACATTAATTGTTATGCAAAATTGCTAATTTGTACTGAATTTTTCATTTTGGGATTCATTGCTGAATTAAGTGTTTCTAAAAATACGGCCAAGTATAAATGTGCCAAGTCCTAGAAATGTTGTTTTATACTCGAAAATCATAGGTTAATGGTTACTATAAGATGAGCCCTACATGATTCATCATTCTGTCCGTTTCTGTATTTACATTTATATAATAATTTAAATATTTATAGTTTAATCTGTTTAGATTTTTCTAATATCGTTTTCACTTTGTCAACTATGTTTACCAGATGTGCGGATGATAAGCCGTATGTGCCTATATATCAGGTTTCCAAAGAAAAGGCGAAAAGCTTGGGACTTGAGTATACTCCTTTGGAAGTGAGCATCAAGGAGACTGTTGAAAGTTTGAAAGAAAAGAAGTTCGTCAACCTTTAA
Protein sequence

>MS.gene058751.t1

MSSNLGNVVCVTGASDDPKKVDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASPFYHDVKDPQAELIDPALKGTLNVLNSCAKSPSLKRLVLTSSIAAVAYNGKPRTPDVVVNETWFTDADLCAKSNLWYVVSKTLAEEAAWKFVKENNIDMVTINPAMVIGPLLQPVLNTSAAAILNLINGAQTFPNSSLGWVNVKDVANAHILAYENASASGRHCLVERVAHYSEVVRILRELYPSLQLPEKCADDKPYVPIYQVSKEKAKSLGLEYTPLEVSIKETVESLKEKKFVNL