AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76722

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76722.t1 MTR_4g028960 96.728 703 21 1 1 703 1 701 0.0 1388
MS.gene76722.t1 MTR_2g018010 36.905 672 384 15 20 667 20 675 1.85e-120 377
MS.gene76722.t1 MTR_5g083030 31.102 717 405 18 1 667 8 685 8.44e-91 298
MS.gene76722.t1 MTR_4g107010 29.784 695 411 19 7 668 16 666 2.95e-81 274
MS.gene76722.t1 MTR_3g078160 29.275 690 424 16 5 668 8 659 3.34e-80 269
MS.gene76722.t1 MTR_3g078160 29.275 690 424 16 5 668 8 659 4.03e-80 269
MS.gene76722.t1 MTR_3g078160 31.900 558 323 13 5 543 8 527 2.79e-73 247
MS.gene76722.t1 MTR_1g094215 31.711 637 397 16 38 667 15 620 7.72e-72 245
MS.gene76722.t1 MTR_4g485520 33.168 606 325 23 95 667 90 648 2.90e-66 231
MS.gene76722.t1 MTR_4g485520 38.861 404 205 13 280 667 103 480 7.35e-65 223
MS.gene76722.t1 MTR_1g094215 38.776 392 218 10 281 667 63 437 4.56e-63 217
MS.gene76722.t1 MTR_4g063800 30.448 647 409 20 35 667 9 628 1.65e-62 221
MS.gene76722.t1 MTR_7g078330 31.570 586 360 16 95 667 85 642 1.32e-61 218
MS.gene76722.t1 MTR_7g059405 37.371 388 222 10 281 665 263 632 3.04e-59 211
MS.gene76722.t1 MTR_5g034440 27.273 693 434 18 7 668 14 667 6.69e-59 211
MS.gene76722.t1 MTR_7g053260 32.391 389 243 9 283 667 79 451 2.55e-49 179
MS.gene76722.t1 MTR_7g116600 32.648 389 243 9 283 667 79 452 1.93e-47 174
MS.gene76722.t1 MTR_1g079450 31.234 397 249 10 273 662 54 433 2.42e-41 157
MS.gene76722.t1 MTR_3g085610 26.773 437 257 10 283 666 281 707 5.40e-37 148
MS.gene76722.t1 MTR_8g080280 26.339 448 247 12 283 667 283 710 1.17e-35 144
MS.gene76722.t1 MTR_2g011140 29.504 383 244 8 287 666 20 379 6.89e-30 122
MS.gene76722.t1 MTR_4g085720 26.415 424 254 14 282 680 14 404 5.01e-26 111
MS.gene76722.t1 MTR_4g091880 28.462 390 247 7 282 666 5 367 6.32e-26 110
MS.gene76722.t1 MTR_8g092870 26.087 414 260 15 278 666 6 398 1.34e-25 110
MS.gene76722.t1 MTR_4g091880 29.412 391 214 15 282 666 5 339 3.77e-24 104
MS.gene76722.t1 MTR_3g466220 55.952 84 36 1 272 354 222 305 1.03e-23 107
MS.gene76722.t1 MTR_3g466220 35.793 271 160 8 408 674 574 834 9.45e-22 101
MS.gene76722.t1 MTR_7g097020 33.117 308 181 10 376 674 472 763 1.04e-23 107
MS.gene76722.t1 MTR_7g097020 59.420 69 28 0 286 354 245 313 2.79e-23 105
MS.gene76722.t1 MTR_3g065080 25.982 331 182 12 279 561 33 348 1.07e-23 105
MS.gene76722.t1 MTR_3g466220 55.952 84 36 1 272 354 201 284 1.09e-23 107
MS.gene76722.t1 MTR_3g466220 35.793 271 160 8 408 674 553 813 1.03e-21 100
MS.gene76722.t1 MTR_1g069845 62.687 67 25 0 283 349 125 191 2.50e-23 105
MS.gene76722.t1 MTR_1g069845 31.457 302 188 10 380 674 317 606 7.67e-21 97.4
MS.gene76722.t1 MTR_1g069845 62.687 67 25 0 283 349 246 312 5.92e-23 104
MS.gene76722.t1 MTR_1g069845 31.788 302 187 10 380 674 438 727 3.93e-21 99.0
MS.gene76722.t1 MTR_1g069845 62.687 67 25 0 283 349 242 308 6.41e-23 104
MS.gene76722.t1 MTR_1g069845 31.788 302 187 10 380 674 434 723 3.39e-21 99.0
MS.gene76722.t1 MTR_5g048050 51.948 77 37 0 277 353 20 96 1.93e-22 101
MS.gene76722.t1 MTR_5g020570 22.907 454 279 11 275 666 281 725 3.83e-22 102
MS.gene76722.t1 MTR_6g071340 51.316 76 37 0 278 353 1 76 4.86e-22 99.8
MS.gene76722.t1 MTR_5g020570 23.128 454 278 12 275 666 266 710 5.21e-22 101
MS.gene76722.t1 MTR_1g093965 29.834 181 95 5 295 470 1 154 7.14e-22 94.7
MS.gene76722.t1 MTR_5g077510 47.368 76 40 0 278 353 31 106 1.02e-21 99.0
MS.gene76722.t1 MTR_8g077205 20.907 507 308 11 229 666 114 596 1.71e-21 99.8
MS.gene76722.t1 MTR_3g103050 30.314 287 190 6 385 667 50 330 1.78e-21 96.7
MS.gene76722.t1 MTR_8g077205 20.907 507 308 11 229 666 226 708 1.85e-21 100
MS.gene76722.t1 MTR_2g087350 32.243 214 121 7 279 486 3 198 1.96e-21 97.8
MS.gene76722.t1 MTR_3g095730 26.379 417 253 18 278 666 9 399 1.72e-20 95.1
MS.gene76722.t1 MTR_7g106340 26.118 425 253 18 279 668 5 403 2.61e-20 94.7
MS.gene76722.t1 MTR_1g093995 29.282 181 96 5 295 470 1 154 2.69e-20 90.1
MS.gene76722.t1 MTR_1g018180 32.500 280 164 8 398 667 72 336 1.49e-19 91.3
MS.gene76722.t1 MTR_3g096370 51.429 70 34 0 279 348 7 76 1.72e-19 92.0
MS.gene76722.t1 MTR_1g056840 26.888 331 166 14 275 559 2 302 1.77e-18 89.0
MS.gene76722.t1 MTR_1g056880 24.714 437 258 16 275 664 2 414 2.95e-18 88.6
MS.gene76722.t1 MTR_5g015210 25.474 369 225 11 276 628 60 394 5.46e-18 87.8
MS.gene76722.t1 MTR_1g056870 37.195 164 79 7 275 432 2 147 4.32e-17 84.7
MS.gene76722.t1 MTR_1g017770 29.515 227 116 8 282 498 45 237 1.29e-16 83.2
MS.gene76722.t1 MTR_2g026130 32.258 279 144 10 408 666 196 449 1.45e-16 83.6
MS.gene76722.t1 MTR_1g100820 50.000 66 33 0 283 348 649 714 9.53e-16 81.6
MS.gene76722.t1 MTR_1g100820 50.000 66 33 0 283 348 647 712 9.68e-16 81.6
MS.gene76722.t1 MTR_1g056910 49.333 75 36 2 279 351 3 77 1.06e-15 80.1
MS.gene76722.t1 MTR_8g039560 30.128 312 182 12 375 665 1 297 2.92e-15 78.2
MS.gene76722.t1 MTR_7g117890 27.907 258 150 8 109 348 82 321 6.55e-15 79.3
MS.gene76722.t1 MTR_7g077780 48.529 68 35 0 283 350 827 894 6.74e-15 79.3
MS.gene76722.t1 MTR_4g051515 34.177 158 85 7 279 433 3 144 5.94e-14 75.1
MS.gene76722.t1 MTR_8g027140 22.489 458 292 15 109 550 83 493 6.43e-14 76.3
MS.gene76722.t1 MTR_3g115670 24.936 389 218 14 264 584 13 395 1.83e-13 74.3
MS.gene76722.t1 MTR_4g125930 46.377 69 36 1 283 351 492 559 3.07e-13 73.2
MS.gene76722.t1 MTR_4g125930 46.377 69 36 1 283 351 692 759 4.05e-13 73.2
MS.gene76722.t1 MTR_5g032010 44.776 67 37 0 282 348 732 798 1.06e-12 72.0
MS.gene76722.t1 MTR_2g096850 44.000 75 41 1 277 350 584 658 1.22e-12 71.6
MS.gene76722.t1 MTR_2g096850 44.000 75 41 1 277 350 735 809 1.23e-12 71.6
MS.gene76722.t1 MTR_1g090320 43.056 72 37 3 283 352 514 583 2.09e-11 68.2
MS.gene76722.t1 MTR_1g090320 43.056 72 37 3 283 352 516 585 2.15e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76722.t1 AT5G01830 53.120 689 276 11 20 701 21 669 0.0 686
MS.gene76722.t1 AT1G29340 35.313 623 361 11 106 696 115 727 1.17e-109 348
MS.gene76722.t1 AT1G60190 30.303 693 411 22 7 668 14 665 4.86e-77 261
MS.gene76722.t1 AT3G54850 31.429 630 381 19 52 667 30 622 4.86e-71 243
MS.gene76722.t1 AT3G46510 28.702 655 416 15 33 669 10 631 1.13e-66 232
MS.gene76722.t1 AT5G42340 31.469 572 346 16 109 667 118 656 1.55e-66 232
MS.gene76722.t1 AT1G23030 31.190 622 353 16 66 667 43 609 1.15e-65 228
MS.gene76722.t1 AT1G10560 28.307 703 423 21 7 668 14 676 1.17e-65 230
MS.gene76722.t1 AT2G28830 29.982 567 364 12 119 668 13 563 1.55e-59 211
MS.gene76722.t1 AT1G71020 30.211 662 370 18 42 667 15 620 3.14e-59 211
MS.gene76722.t1 AT1G71020 33.676 487 269 13 207 667 14 472 4.91e-59 207
MS.gene76722.t1 AT3G07360 32.927 410 246 13 271 667 59 452 4.19e-48 176
MS.gene76722.t1 AT1G67530 26.765 680 376 23 90 666 46 706 8.46e-41 160
MS.gene76722.t1 AT1G67530 26.765 680 376 23 90 666 46 706 8.46e-41 160
MS.gene76722.t1 AT1G67530 26.765 680 376 23 90 666 46 706 8.46e-41 160
MS.gene76722.t1 AT1G27910 24.405 672 399 20 90 666 46 703 4.55e-36 145
MS.gene76722.t1 AT1G24330 23.923 673 403 18 90 666 46 705 8.03e-34 139
MS.gene76722.t1 AT1G24330 23.923 673 403 18 90 666 46 705 8.03e-34 139
MS.gene76722.t1 AT4G21350 32.051 390 232 10 282 665 5 367 3.58e-32 129
MS.gene76722.t1 AT1G67530 27.368 475 235 18 90 469 46 505 4.28e-29 122
MS.gene76722.t1 AT1G49780 29.484 407 252 12 278 666 10 399 5.12e-28 117
MS.gene76722.t1 AT5G18320 28.856 402 253 11 282 671 72 452 1.33e-27 117
MS.gene76722.t1 AT3G19380 29.282 362 222 12 278 622 10 354 1.55e-27 116
MS.gene76722.t1 AT5G67340 32.862 283 168 9 391 666 430 697 7.75e-25 110
MS.gene76722.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.92e-20 96.7
MS.gene76722.t1 AT5G67340 32.862 283 168 9 391 666 430 697 7.75e-25 110
MS.gene76722.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.92e-20 96.7
MS.gene76722.t1 AT1G66160 25.629 437 262 16 275 686 26 424 8.31e-24 105
MS.gene76722.t1 AT5G37490 24.939 413 255 14 279 665 28 411 9.50e-24 105
MS.gene76722.t1 AT2G23140 32.862 283 174 8 397 674 556 827 6.46e-23 104
MS.gene76722.t1 AT2G23140 30.859 256 145 7 119 366 86 317 1.26e-22 103
MS.gene76722.t1 AT2G23140 32.862 283 174 8 397 674 553 824 6.67e-23 104
MS.gene76722.t1 AT2G23140 30.859 256 145 7 119 366 83 314 1.40e-22 103
MS.gene76722.t1 AT2G23140 32.862 283 174 8 397 674 553 824 6.67e-23 104
MS.gene76722.t1 AT2G23140 30.859 256 145 7 119 366 83 314 1.40e-22 103
MS.gene76722.t1 AT2G23140 33.216 283 173 9 397 674 364 635 1.50e-22 103
MS.gene76722.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.26e-21 100
MS.gene76722.t1 AT2G23140 33.216 283 173 9 397 674 364 635 1.50e-22 103
MS.gene76722.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.26e-21 100
MS.gene76722.t1 AT3G54790 54.795 73 33 0 282 354 202 274 7.20e-22 101
MS.gene76722.t1 AT3G54790 30.515 272 158 7 408 666 459 712 2.27e-18 90.1
MS.gene76722.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.68e-22 100
MS.gene76722.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.41e-18 90.9
MS.gene76722.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.68e-22 100
MS.gene76722.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.41e-18 90.9
MS.gene76722.t1 AT5G64660 50.667 75 37 0 274 348 2 76 1.29e-20 95.5
MS.gene76722.t1 AT3G49810 26.331 357 224 10 282 628 64 391 3.31e-20 94.7
MS.gene76722.t1 AT3G01400 29.180 305 183 8 385 675 63 348 1.85e-19 91.3
MS.gene76722.t1 AT2G35930 31.551 187 97 5 279 458 9 171 2.00e-19 91.7
MS.gene76722.t1 AT3G52450 52.778 72 33 1 279 349 4 75 2.34e-19 92.0
MS.gene76722.t1 AT5G65920 25.070 359 226 10 282 628 60 387 3.47e-19 91.3
MS.gene76722.t1 AT5G65920 25.070 359 226 10 282 628 60 387 3.47e-19 91.3
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G07360 28.829 333 212 12 344 667 1 317 5.69e-19 89.0
MS.gene76722.t1 AT3G18710 50.725 69 34 0 280 348 10 78 8.83e-19 89.7
MS.gene76722.t1 AT5G09800 52.239 67 32 0 282 348 11 77 1.11e-18 89.4
MS.gene76722.t1 AT5G18340 26.780 295 193 9 271 557 63 342 2.37e-18 89.0
MS.gene76722.t1 AT5G58680 29.496 278 182 7 395 667 71 339 3.94e-17 84.0
MS.gene76722.t1 AT1G01660 51.515 66 32 0 283 348 498 563 2.81e-16 83.2
MS.gene76722.t1 AT1G01660 51.515 66 32 0 283 348 499 564 2.89e-16 83.2
MS.gene76722.t1 AT1G66160 23.799 437 254 17 275 686 26 408 4.86e-15 78.2
MS.gene76722.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.55e-15 79.3
MS.gene76722.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.55e-15 79.3
MS.gene76722.t1 AT1G01680 44.737 76 42 0 273 348 224 299 9.31e-15 76.3
MS.gene76722.t1 AT2G45920 48.485 66 34 0 283 348 326 391 1.95e-14 76.3
MS.gene76722.t1 AT3G11840 44.737 76 40 2 279 353 7 81 2.00e-13 73.6
MS.gene76722.t1 AT1G08315 26.888 331 188 14 377 677 4 310 6.09e-13 70.9
MS.gene76722.t1 AT1G56040 46.032 63 34 0 283 345 354 416 8.65e-13 71.2
MS.gene76722.t1 AT3G49060 41.892 74 43 0 275 348 728 801 1.10e-12 72.0
MS.gene76722.t1 AT3G49060 41.892 74 43 0 275 348 695 768 1.10e-12 72.0
MS.gene76722.t1 AT3G49060 41.892 74 43 0 275 348 718 791 1.22e-12 72.0
MS.gene76722.t1 AT4G16490 30.824 279 150 10 408 666 206 461 1.73e-12 70.9
MS.gene76722.t1 AT5G57035 43.939 66 37 0 283 348 709 774 2.11e-12 71.2
MS.gene76722.t1 AT3G61390 43.939 66 37 0 283 348 279 344 3.66e-12 68.9
MS.gene76722.t1 AT3G61390 43.939 66 37 0 283 348 354 419 6.11e-12 68.9
MS.gene76722.t1 AT2G19410 44.928 69 37 1 280 348 709 776 1.91e-11 68.2
MS.gene76722.t1 AT2G19410 44.928 69 37 1 280 348 744 811 2.11e-11 67.8
MS.gene76722.t1 AT2G19410 44.928 69 37 1 280 348 723 790 2.18e-11 67.8
MS.gene76722.t1 AT5G40140 25.773 291 183 10 391 666 234 506 3.07e-11 67.0

Find 173 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTAAAGTTAGTGATATT+TGG 0.169459 4.1:-70967548 None:intergenic
CGTTTATGAGCTTCGGGTTT+TGG 0.200615 4.1:+70968693 MS.gene76722:CDS
ACACCATGGAGCTGCGTTTA+AGG 0.211733 4.1:-70967609 None:intergenic
AGCTGGAGCTATAGAGATTT+TGG 0.212053 4.1:+70968753 MS.gene76722:CDS
ATTAAGCTTTGGATGGATTC+TGG 0.220926 4.1:+70968412 MS.gene76722:CDS
GGTTAAAGTTAGTGATATTT+GGG 0.221812 4.1:-70967547 None:intergenic
TTCAAGTTCATTTCTTGAAT+TGG 0.244410 4.1:-70967923 None:intergenic
GCTACTGTGTTTAGTTTAAC+TGG 0.265467 4.1:+70968964 MS.gene76722:CDS
CTGCGTTTAAGGAACATATT+TGG 0.265739 4.1:-70967598 None:intergenic
GATAATTTCAACAAATTAAC+AGG 0.267688 4.1:+70967872 MS.gene76722:CDS
CATACCACCGTTAACTTTAA+CGG 0.270519 4.1:-70968563 None:intergenic
AAGGGTGATTTGGGAGTTAA+TGG 0.283097 4.1:+70969398 MS.gene76722:CDS
TGAGAAATATGATAGCTATT+TGG 0.284416 4.1:+70968500 MS.gene76722:CDS
CTGATATTGAATTACGAATT+AGG 0.306908 4.1:-70967703 None:intergenic
ACGTCGGTGGGCGGCGGGTT+TGG 0.311205 4.1:+70969473 MS.gene76722:CDS
TGGTGTGGAAAGGGTGATTT+GGG 0.315581 4.1:+70969389 MS.gene76722:CDS
TGGTGGTTCCGGCGGATTAC+CGG 0.317373 4.1:+70968317 MS.gene76722:CDS
ATGGTGTGGAAAGGGTGATT+TGG 0.329462 4.1:+70969388 MS.gene76722:CDS
CGGGAAATGATGGCCGTTTA+CGG 0.340552 4.1:+70968275 MS.gene76722:CDS
GGCGGTTGCGGCAGCGTTTG+TGG 0.341028 4.1:+70969239 MS.gene76722:CDS
GGAGATGACAGCGGGGATTA+TGG 0.342183 4.1:+70969155 MS.gene76722:CDS
GCGGTTGCGGCAGCGTTTGT+GGG 0.350026 4.1:+70969240 MS.gene76722:CDS
ATGGTGTCGTTTATGAGCTT+CGG 0.355525 4.1:+70968686 MS.gene76722:CDS
CTGCTCGAAGATTGTTCTAA+TGG 0.358850 4.1:+70967809 MS.gene76722:CDS
AAAATTGGGGACGGTGTTAA+GGG 0.365091 4.1:+70969269 MS.gene76722:CDS
AAATGTATATTGTGCTTCAT+AGG 0.366592 4.1:+70967777 MS.gene76722:CDS
TGGTGTCGTTTATGAGCTTC+GGG 0.367648 4.1:+70968687 MS.gene76722:CDS
GTCGTTGGAATTGGTGGTTC+CGG 0.372795 4.1:+70968306 MS.gene76722:CDS
CGCATCAGTTCAAGACTTAT+CGG 0.383935 4.1:-70968343 None:intergenic
GAACAGAAAATTCCATTTGA+AGG 0.385995 4.1:+70968529 MS.gene76722:CDS
TCAGAAACGAAGAATTTGTT+AGG 0.387591 4.1:+70967989 MS.gene76722:CDS
AGCGAATAAGACAAGGATAA+TGG 0.391442 4.1:+70968864 MS.gene76722:CDS
TTAAGCTTTGGATGGATTCT+GGG 0.392841 4.1:+70968413 MS.gene76722:CDS
GAACAGAAACAACGTCGTTT+CGG 0.392901 4.1:-70968030 None:intergenic
GCGGAATCAGTCGTTGGAAT+TGG 0.393295 4.1:+70968297 MS.gene76722:CDS
ATGGCGGTGGGGAGTGTTAG+AGG 0.398283 4.1:+70969417 MS.gene76722:CDS
GCGGAGCTTGCGGCAATTCA+TGG 0.399152 4.1:+70969369 MS.gene76722:CDS
GATCCTGTCGTCGTGGCAAC+AGG 0.400951 4.1:+70968367 MS.gene76722:CDS
GATTTGGGAGTTAATGGCGG+TGG 0.402348 4.1:+70969404 MS.gene76722:CDS
GTATTAACTCCGTGTGGGCC+AGG 0.406263 4.1:-70968465 None:intergenic
ATCGTGTTTCGATTAAGCTT+TGG 0.414902 4.1:+70968401 MS.gene76722:CDS
TGGGCGGCGGGTTTGGATGG+TGG 0.427448 4.1:+70969480 MS.gene76722:CDS
GGCGGTAATGCCGGAGGAGG+CGG 0.437841 4.1:+70969176 MS.gene76722:CDS
GATGATGGTGGTTTAGTAGT+TGG 0.439712 4.1:-70967503 None:intergenic
ACGACTGATTCCGCCGTAAA+CGG 0.442880 4.1:-70968288 None:intergenic
GACGTTGTTTCTGTTCTAGA+AGG 0.453096 4.1:+70968037 MS.gene76722:CDS
GGGAGGGATCAGAGAGGGCG+AGG 0.456168 4.1:+70969289 MS.gene76722:CDS
CACAACACGTGTCCTAAAAC+AGG 0.458381 4.1:+70968436 MS.gene76722:CDS
GGGGATTATGGCGGTAATGC+CGG 0.458721 4.1:+70969167 MS.gene76722:CDS
CGGTGGGGAGTGTTAGAGGG+AGG 0.459782 4.1:+70969421 MS.gene76722:CDS
GAACTTGAATGATGATGTAT+TGG 0.460758 4.1:+70967937 MS.gene76722:CDS
TATGACAAATAAGACAGCTT+TGG 0.462456 4.1:+70968582 MS.gene76722:CDS
GGGGCCGTGGAGATGACAGC+GGG 0.467255 4.1:+70969147 MS.gene76722:CDS
ATTTGGGAGTTAATGGCGGT+GGG 0.475030 4.1:+70969405 MS.gene76722:CDS
ATATTTCTCAAAGCATGGTT+AGG 0.475353 4.1:-70968487 None:intergenic
GGTTAAGCGTGGAGGATTAG+TGG 0.478476 4.1:+70969218 MS.gene76722:CDS
TTCAAGAAGAAATTCAGAAT+CGG 0.481818 4.1:+70968161 MS.gene76722:CDS
CGAGCTTGTATAGCTGAAGC+TGG 0.483143 4.1:+70968736 MS.gene76722:CDS
AACGACGCCGTTTCTGATGA+TGG 0.489185 4.1:-70967518 None:intergenic
AGCGAAGAGGGACGCATTAG+CGG 0.492282 4.1:+70969071 MS.gene76722:CDS
GCGTCCCTCTTCGCTCCCTC+AGG 0.492921 4.1:-70969063 None:intergenic
TGTCCTGTTGCCACGACGAC+AGG 0.493205 4.1:-70968370 None:intergenic
AGTATCGTCACCGCCTCCTC+CGG 0.493654 4.1:-70969186 None:intergenic
TCAGAGATTGTTGCGTTGAT+CGG 0.493804 4.1:+70968202 MS.gene76722:CDS
GAGTGTTAGAGGGAGGAGGA+AGG 0.494415 4.1:+70969428 MS.gene76722:CDS
TTTGCATCTCGCAGCGGATA+GGG 0.494746 4.1:+70969098 MS.gene76722:CDS
CGTTAGTGGATTGGTTGAGT+TGG 0.496851 4.1:+70969029 MS.gene76722:CDS
TGTGCTAATGAAGTTGTAGT+TGG 0.496874 4.1:-70969564 None:intergenic
GTTGGTAGAGAGTGGGGCCG+TGG 0.498825 4.1:+70969134 MS.gene76722:CDS
GGGTTTGGATGGTGGTGAAA+CGG 0.499778 4.1:+70969488 MS.gene76722:CDS
TATTAACTCCGTGTGGGCCA+GGG 0.501113 4.1:-70968464 None:intergenic
GCTACCGTTTCACCTTCAAA+TGG 0.501489 4.1:-70968541 None:intergenic
TGTGTTTAGTTTAACTGGTG+TGG 0.502982 4.1:+70968969 MS.gene76722:CDS
GGTGCAACGACGGCGTCGAC+GGG 0.502990 4.1:+70968256 MS.gene76722:CDS
GACACGAATCGTTAGTGGAT+TGG 0.503002 4.1:+70969020 MS.gene76722:CDS
CGGTGGGCGGCGGGTTTGGA+TGG 0.505339 4.1:+70969477 MS.gene76722:CDS
TGGAGGATTAGTGGCGGTTG+CGG 0.506660 4.1:+70969227 MS.gene76722:CDS
AATTCCATTTGAAGGTGAAA+CGG 0.509042 4.1:+70968537 MS.gene76722:CDS
ACGGTAGCCGTTAAAGTTAA+CGG 0.510244 4.1:+70968556 MS.gene76722:CDS
TGGTGCAACGACGGCGTCGA+CGG 0.513053 4.1:+70968255 MS.gene76722:CDS
AGGAAGACACGAATCGTTAG+TGG 0.516476 4.1:+70969015 MS.gene76722:CDS
GAATCAGTCGTTGGAATTGG+TGG 0.520402 4.1:+70968300 MS.gene76722:CDS
TAGGGAGACGGTGGGAAGGT+TGG 0.520508 4.1:+70969116 MS.gene76722:CDS
TTCACAAAATCCGAGCCTAC+AGG 0.523516 4.1:+70968801 MS.gene76722:CDS
TGGGGAGTGTTAGAGGGAGG+AGG 0.524460 4.1:+70969424 MS.gene76722:CDS
GGAGGGATCAGAGAGGGCGA+GGG 0.524599 4.1:+70969290 MS.gene76722:CDS
ACGGCGTCGACGGGAAATGA+TGG 0.528688 4.1:+70968265 MS.gene76722:CDS
TATGGCGGTAATGCCGGAGG+AGG 0.529298 4.1:+70969173 MS.gene76722:CDS
TTGTGAGGTGTAATAGAGAA+TGG 0.534465 4.1:-70967574 None:intergenic
ATTGGGGACGGTGTTAAGGG+AGG 0.534820 4.1:+70969272 MS.gene76722:CDS
TGGACCTGAGGGAGCGAAGA+GGG 0.535333 4.1:+70969059 MS.gene76722:CDS
TAACGGTTCTTTAACGTCGA+TGG 0.539368 4.1:+70968654 MS.gene76722:CDS
ATACGTAAAACGCACCTCCT+CGG 0.542514 4.1:+70967665 MS.gene76722:CDS
GATAAGTCTTGAACTGATGC+GGG 0.544369 4.1:+70968345 MS.gene76722:CDS
TGTTTCGATTAAGCTTTGGA+TGG 0.545411 4.1:+70968405 MS.gene76722:CDS
AGAGATTGTGGCGGAGCTTG+CGG 0.545486 4.1:+70969359 MS.gene76722:CDS
TTGGATGGTGGTGAAACGGA+GGG 0.546989 4.1:+70969492 MS.gene76722:CDS
TGGGAAGGTTGGTAGAGAGT+GGG 0.549322 4.1:+70969127 MS.gene76722:CDS
GGTGTAATAGAGAATGGAGG+AGG 0.550947 4.1:-70967568 None:intergenic
GGAAGGCGGCGACGTTGTTG+AGG 0.554359 4.1:+70969445 MS.gene76722:CDS
CGATAAGTCTTGAACTGATG+CGG 0.556842 4.1:+70968344 MS.gene76722:CDS
CGGCAATTCATGGTGTGGAA+AGG 0.558496 4.1:+70969379 MS.gene76722:CDS
TAAACCACCATCATCAGAAA+CGG 0.559799 4.1:+70967511 MS.gene76722:CDS
GTGGGAAGGTTGGTAGAGAG+TGG 0.562347 4.1:+70969126 MS.gene76722:CDS
AGGAACATATTTGGTTTGTG+AGG 0.564288 4.1:-70967589 None:intergenic
CTATCATATTTCTCAAAGCA+TGG 0.564762 4.1:-70968492 None:intergenic
ATCGGACACCGGTAATCCGC+CGG 0.567196 4.1:-70968325 None:intergenic
GGCAATTCATGGTGTGGAAA+GGG 0.572572 4.1:+70969380 MS.gene76722:CDS
TTAGTAGTTGGAAATGTTTG+AGG 0.577855 4.1:-70967491 None:intergenic
GTTCCTTAAACGCAGCTCCA+TGG 0.581251 4.1:+70967606 MS.gene76722:CDS
GGGAAGGTTGGTAGAGAGTG+GGG 0.582589 4.1:+70969128 MS.gene76722:CDS
AGAGAGGGCGAGGGAGAGTG+CGG 0.583035 4.1:+70969299 MS.gene76722:CDS
ACGTCGATGGAAGAGACAAA+TGG 0.584346 4.1:+70968667 MS.gene76722:CDS
GGTGATTTGGGAGTTAATGG+CGG 0.585091 4.1:+70969401 MS.gene76722:CDS
CGGATAGGGAGACGGTGGGA+AGG 0.585973 4.1:+70969112 MS.gene76722:CDS
CGCAGCGGATAGGGAGACGG+TGG 0.589169 4.1:+70969107 MS.gene76722:CDS
GTTGGAATTGGTGGTTCCGG+CGG 0.589268 4.1:+70968309 MS.gene76722:CDS
TGTTAGAGGGAGGAGGAAGG+CGG 0.591852 4.1:+70969431 MS.gene76722:CDS
TCCTAAAACAGGTCAAACCC+TGG 0.592266 4.1:+70968447 MS.gene76722:CDS
AGGTCAAACCCTGGCCCACA+CGG 0.592317 4.1:+70968456 MS.gene76722:CDS
CGGTTGCGGCAGCGTTTGTG+GGG 0.593066 4.1:+70969241 MS.gene76722:CDS
TGAGGTGTAATAGAGAATGG+AGG 0.594765 4.1:-70967571 None:intergenic
TTTGGATGGTGGTGAAACGG+AGG 0.595577 4.1:+70969491 MS.gene76722:CDS
TTTACGGCGGAATCAGTCGT+TGG 0.597846 4.1:+70968291 MS.gene76722:CDS
GGTTAGGTATTAACTCCGTG+TGG 0.601915 4.1:-70968471 None:intergenic
TGGCGGTGGGGAGTGTTAGA+GGG 0.603345 4.1:+70969418 MS.gene76722:CDS
AACAAGTTGACGTTGACTAA+CGG 0.605175 4.1:+70968637 MS.gene76722:CDS
GAAAGGTGGATCAGAGATTG+TGG 0.605941 4.1:+70969347 MS.gene76722:CDS
AATCCCCGCTGTCATCTCCA+CGG 0.607104 4.1:-70969151 None:intergenic
GTTAAAGTTAGTGATATTTG+GGG 0.607708 4.1:-70967546 None:intergenic
TGTTAGTACATCAACTACCA+TGG 0.608462 4.1:+70969536 MS.gene76722:CDS
ACTGCATTAACCTGTAGGCT+CGG 0.609903 4.1:-70968811 None:intergenic
GTTAAGGGAGGGATCAGAGA+GGG 0.610803 4.1:+70969284 MS.gene76722:CDS
TTTAAGATCGGGTGCCACGT+GGG 0.611826 4.1:+70968921 MS.gene76722:CDS
TTGTAACTGCATTAACCTGT+AGG 0.615427 4.1:-70968816 None:intergenic
TTGGGGACGGTGTTAAGGGA+GGG 0.620299 4.1:+70969273 MS.gene76722:CDS
GTGACAATGTGTAGGAAAGG+TGG 0.623592 4.1:+70969333 MS.gene76722:CDS
TTTGGAAGCAACAAGAATGA+CGG 0.625454 4.1:+70968600 MS.gene76722:CDS
TCTCGCAGCGGATAGGGAGA+CGG 0.625914 4.1:+70969104 MS.gene76722:CDS
GATTATGGCGGTAATGCCGG+AGG 0.627766 4.1:+70969170 MS.gene76722:CDS
GCTTGCGGCAATTCATGGTG+TGG 0.630345 4.1:+70969374 MS.gene76722:CDS
AAGATCGGGTGCCACGTGGG+AGG 0.630647 4.1:+70968924 MS.gene76722:CDS
CGACGCTTGTGACAATGTGT+AGG 0.639061 4.1:+70969325 MS.gene76722:CDS
TTTGGGAGTTAATGGCGGTG+GGG 0.641101 4.1:+70969406 MS.gene76722:CDS
CTTGAAGCAGTGGTTAAGCG+TGG 0.642024 4.1:+70969207 MS.gene76722:CDS
GGTGACGATACTTGAAGCAG+TGG 0.647555 4.1:+70969197 MS.gene76722:CDS
GTTAGGTATTAACTCCGTGT+GGG 0.650428 4.1:-70968470 None:intergenic
ACAAGGATAATGGAGACTGA+AGG 0.650963 4.1:+70968874 MS.gene76722:CDS
TGGGGCCGTGGAGATGACAG+CGG 0.650969 4.1:+70969146 MS.gene76722:CDS
GCAGCGGATAGGGAGACGGT+GGG 0.651330 4.1:+70969108 MS.gene76722:CDS
TTTAAAAGGAAACGACACCA+TGG 0.659696 4.1:-70967623 None:intergenic
TACTTGAAGCGAATAAGACA+AGG 0.661909 4.1:+70968857 MS.gene76722:CDS
CTTGTGACAATGTGTAGGAA+AGG 0.662139 4.1:+70969330 MS.gene76722:CDS
GTGGGAGGCTAAAGCAAATG+CGG 0.672161 4.1:+70968939 MS.gene76722:CDS
ATGGACCTGAGGGAGCGAAG+AGG 0.672284 4.1:+70969058 MS.gene76722:CDS
GACGCCGTTTCTGATGATGG+TGG 0.677070 4.1:-70967515 None:intergenic
GATGCGGGATCCTGTCGTCG+TGG 0.685189 4.1:+70968360 MS.gene76722:CDS
TAAGCGTGGAGGATTAGTGG+CGG 0.685593 4.1:+70969221 MS.gene76722:CDS
GGAGGCTAAAGCAAATGCGG+CGG 0.686035 4.1:+70968942 MS.gene76722:CDS
GATGACAGCGGGGATTATGG+CGG 0.687573 4.1:+70969158 MS.gene76722:CDS
GAAATGATGGCCGTTTACGG+CGG 0.687834 4.1:+70968278 MS.gene76722:CDS
GTTCAAGACTTATCGGACAC+CGG 0.688916 4.1:-70968336 None:intergenic
ATTTGCTTTAGCCTCCCACG+TGG 0.692219 4.1:-70968935 None:intergenic
AGTTGTAGTTGGAACAACCA+TGG 0.693372 4.1:-70969553 None:intergenic
GAAGCAGTGGTTAAGCGTGG+AGG 0.694054 4.1:+70969210 MS.gene76722:CDS
ACACATTGTCACAAGCGTCG+CGG 0.697841 4.1:-70969322 None:intergenic
CATCGAAAACGACACCGAGG+AGG 0.702271 4.1:-70967679 None:intergenic
AGGTGGATCAGAGATTGTGG+CGG 0.710219 4.1:+70969350 MS.gene76722:CDS
TGTTAAGGGAGGGATCAGAG+AGG 0.725744 4.1:+70969283 MS.gene76722:CDS
GGAAGCAACAAGAATGACGG+CGG 0.729408 4.1:+70968603 MS.gene76722:CDS
GGGCCGTGGAGATGACAGCG+GGG 0.731568 4.1:+70969148 MS.gene76722:CDS
GTAGCCGTTAAAGTTAACGG+TGG 0.777768 4.1:+70968559 MS.gene76722:CDS
GGTCATCGAAAACGACACCG+AGG 0.788291 4.1:-70967682 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTTCAACAAATTAAC+AGG + chr4.1:70967872-70967891 MS.gene76722:CDS 20.0%
!!! AAGAGGAAATTTTGTTTAAA+AGG - chr4.1:70967640-70967659 None:intergenic 20.0%
!!! ATCTTCAATTTTTGAGAAAT+TGG + chr4.1:70968096-70968115 MS.gene76722:CDS 20.0%
! AAATGTATATTGTGCTTCAT+AGG + chr4.1:70967777-70967796 MS.gene76722:CDS 25.0%
! AGGTTAAAGTTAGTGATATT+TGG - chr4.1:70967551-70967570 None:intergenic 25.0%
! CTGATATTGAATTACGAATT+AGG - chr4.1:70967706-70967725 None:intergenic 25.0%
! GGTTAAAGTTAGTGATATTT+GGG - chr4.1:70967550-70967569 None:intergenic 25.0%
! GTTAAAGTTAGTGATATTTG+GGG - chr4.1:70967549-70967568 None:intergenic 25.0%
! TGAGAAATATGATAGCTATT+TGG + chr4.1:70968500-70968519 MS.gene76722:CDS 25.0%
! TTCAAGAAGAAATTCAGAAT+CGG + chr4.1:70968161-70968180 MS.gene76722:CDS 25.0%
! TTTGTGGGGATAAAAAAATT+GGG + chr4.1:70969255-70969274 MS.gene76722:CDS 25.0%
!! GAAGATAGTTTTTCATCTTT+TGG - chr4.1:70968082-70968101 None:intergenic 25.0%
!! TTCAAGTTCATTTCTTGAAT+TGG - chr4.1:70967926-70967945 None:intergenic 25.0%
AATTCCATTTGAAGGTGAAA+CGG + chr4.1:70968537-70968556 MS.gene76722:CDS 30.0%
ATATTTCTCAAAGCATGGTT+AGG - chr4.1:70968490-70968509 None:intergenic 30.0%
CTATCATATTTCTCAAAGCA+TGG - chr4.1:70968495-70968514 None:intergenic 30.0%
GAACAGAAAATTCCATTTGA+AGG + chr4.1:70968529-70968548 MS.gene76722:CDS 30.0%
GTTTGTGGGGATAAAAAAAT+TGG + chr4.1:70969254-70969273 MS.gene76722:CDS 30.0%
TATGACAAATAAGACAGCTT+TGG + chr4.1:70968582-70968601 MS.gene76722:CDS 30.0%
TTAGTAGTTGGAAATGTTTG+AGG - chr4.1:70967494-70967513 None:intergenic 30.0%
TTGTGGGGATAAAAAAATTG+GGG + chr4.1:70969256-70969275 MS.gene76722:CDS 30.0%
! GAACTTGAATGATGATGTAT+TGG + chr4.1:70967937-70967956 MS.gene76722:CDS 30.0%
! TCAGAAACGAAGAATTTGTT+AGG + chr4.1:70967989-70968008 MS.gene76722:CDS 30.0%
AGCGAATAAGACAAGGATAA+TGG + chr4.1:70968864-70968883 MS.gene76722:CDS 35.0%
ATCGTGTTTCGATTAAGCTT+TGG + chr4.1:70968401-70968420 MS.gene76722:CDS 35.0%
CATACCACCGTTAACTTTAA+CGG - chr4.1:70968566-70968585 None:intergenic 35.0%
CTGCGTTTAAGGAACATATT+TGG - chr4.1:70967601-70967620 None:intergenic 35.0%
TAAACCACCATCATCAGAAA+CGG + chr4.1:70967511-70967530 MS.gene76722:CDS 35.0%
TACTTGAAGCGAATAAGACA+AGG + chr4.1:70968857-70968876 MS.gene76722:CDS 35.0%
TGTGCTAATGAAGTTGTAGT+TGG - chr4.1:70969567-70969586 None:intergenic 35.0%
TGTTAGTACATCAACTACCA+TGG + chr4.1:70969536-70969555 MS.gene76722:CDS 35.0%
TTGTAACTGCATTAACCTGT+AGG - chr4.1:70968819-70968838 None:intergenic 35.0%
TTGTGAGGTGTAATAGAGAA+TGG - chr4.1:70967577-70967596 None:intergenic 35.0%
TTTAAAAGGAAACGACACCA+TGG - chr4.1:70967626-70967645 None:intergenic 35.0%
TTTGGAAGCAACAAGAATGA+CGG + chr4.1:70968600-70968619 MS.gene76722:CDS 35.0%
! AACAAGTTGACGTTGACTAA+CGG + chr4.1:70968637-70968656 MS.gene76722:CDS 35.0%
! GCTACTGTGTTTAGTTTAAC+TGG + chr4.1:70968964-70968983 MS.gene76722:CDS 35.0%
! TGTGTTTAGTTTAACTGGTG+TGG + chr4.1:70968969-70968988 MS.gene76722:CDS 35.0%
!! AGGAACATATTTGGTTTGTG+AGG - chr4.1:70967592-70967611 None:intergenic 35.0%
!! ATTAAGCTTTGGATGGATTC+TGG + chr4.1:70968412-70968431 MS.gene76722:CDS 35.0%
!! TGTTTCGATTAAGCTTTGGA+TGG + chr4.1:70968405-70968424 MS.gene76722:CDS 35.0%
!! TTAAGCTTTGGATGGATTCT+GGG + chr4.1:70968413-70968432 MS.gene76722:CDS 35.0%
!!! CGGTTTTTAAGTTACGAAGT+TGG + chr4.1:70968778-70968797 MS.gene76722:CDS 35.0%
!!! GAGCTATAGAGATTTTGGTT+CGG + chr4.1:70968758-70968777 MS.gene76722:CDS 35.0%
!!! TGTAGCAGAGGTTTTAAGAT+CGG + chr4.1:70968909-70968928 MS.gene76722:CDS 35.0%
ACGGTAGCCGTTAAAGTTAA+CGG + chr4.1:70968556-70968575 MS.gene76722:CDS 40.0%
AGTTGTAGTTGGAACAACCA+TGG - chr4.1:70969556-70969575 None:intergenic 40.0%
CGATAAGTCTTGAACTGATG+CGG + chr4.1:70968344-70968363 MS.gene76722:CDS 40.0%
CGCATCAGTTCAAGACTTAT+CGG - chr4.1:70968346-70968365 None:intergenic 40.0%
CTGCTCGAAGATTGTTCTAA+TGG + chr4.1:70967809-70967828 MS.gene76722:CDS 40.0%
CTTGTGACAATGTGTAGGAA+AGG + chr4.1:70969330-70969349 MS.gene76722:CDS 40.0%
GAACAGAAACAACGTCGTTT+CGG - chr4.1:70968033-70968052 None:intergenic 40.0%
GATAAGTCTTGAACTGATGC+GGG + chr4.1:70968345-70968364 MS.gene76722:CDS 40.0%
GGGGATAAAAAAATTGGGGA+CGG + chr4.1:70969260-70969279 MS.gene76722:CDS 40.0%
GTTAGGTATTAACTCCGTGT+GGG - chr4.1:70968473-70968492 None:intergenic 40.0%
TAACGGTTCTTTAACGTCGA+TGG + chr4.1:70968654-70968673 MS.gene76722:CDS 40.0%
TGAGGTGTAATAGAGAATGG+AGG - chr4.1:70967574-70967593 None:intergenic 40.0%
! AAAAATTGGGGACGGTGTTA+AGG + chr4.1:70969268-70969287 MS.gene76722:CDS 40.0%
! AAAATTGGGGACGGTGTTAA+GGG + chr4.1:70969269-70969288 MS.gene76722:CDS 40.0%
! AAGGGTGATTTGGGAGTTAA+TGG + chr4.1:70969398-70969417 MS.gene76722:CDS 40.0%
! AGCTGGAGCTATAGAGATTT+TGG + chr4.1:70968753-70968772 MS.gene76722:CDS 40.0%
! CGTTGTTGAGGATTTTACGT+CGG + chr4.1:70969457-70969476 MS.gene76722:CDS 40.0%
! TATGCGAAGTGCGTTCTTTT+TGG + chr4.1:70968235-70968254 MS.gene76722:CDS 40.0%
! TCAGAGATTGTTGCGTTGAT+CGG + chr4.1:70968202-70968221 MS.gene76722:CDS 40.0%
!! ACAAGGATAATGGAGACTGA+AGG + chr4.1:70968874-70968893 MS.gene76722:CDS 40.0%
!! ATGGTGTCGTTTATGAGCTT+CGG + chr4.1:70968686-70968705 MS.gene76722:CDS 40.0%
!! CGTTTTACGTATAGTGGAAG+AGG - chr4.1:70967657-70967676 None:intergenic 40.0%
!! CTTTTAGAAAGCGGTTAGGT+AGG + chr4.1:70968995-70969014 MS.gene76722:CDS 40.0%
!! GACGTTGTTTCTGTTCTAGA+AGG + chr4.1:70968037-70968056 MS.gene76722:CDS 40.0%
!! GATGATGGTGGTTTAGTAGT+TGG - chr4.1:70967506-70967525 None:intergenic 40.0%
!! TGTTTTAAGTGGTGTAGCAG+AGG + chr4.1:70968897-70968916 MS.gene76722:CDS 40.0%
!! TTGGTTGAGTTGGCGAAAAA+TGG + chr4.1:70969039-70969058 MS.gene76722:CDS 40.0%
!!! GAGACTGAAGGTGTTTTAAG+TGG + chr4.1:70968886-70968905 MS.gene76722:CDS 40.0%
!!! GTAGCAGAGGTTTTAAGATC+GGG + chr4.1:70968910-70968929 MS.gene76722:CDS 40.0%
!!! GTGTGGTTGCTTTTAGAAAG+CGG + chr4.1:70968986-70969005 MS.gene76722:CDS 40.0%
!!! GTTGCTTTTAGAAAGCGGTT+AGG + chr4.1:70968991-70969010 MS.gene76722:CDS 40.0%
ACGTCGATGGAAGAGACAAA+TGG + chr4.1:70968667-70968686 MS.gene76722:CDS 45.0%
ACTGCATTAACCTGTAGGCT+CGG - chr4.1:70968814-70968833 None:intergenic 45.0%
AGGAAGACACGAATCGTTAG+TGG + chr4.1:70969015-70969034 MS.gene76722:CDS 45.0%
ATACGTAAAACGCACCTCCT+CGG + chr4.1:70967665-70967684 MS.gene76722:CDS 45.0%
ATGGTGTGGAAAGGGTGATT+TGG + chr4.1:70969388-70969407 MS.gene76722:CDS 45.0%
ATTTGGGAGTTAATGGCGGT+GGG + chr4.1:70969405-70969424 MS.gene76722:CDS 45.0%
CACAACACGTGTCCTAAAAC+AGG + chr4.1:70968436-70968455 MS.gene76722:CDS 45.0%
GAAAGGTGGATCAGAGATTG+TGG + chr4.1:70969347-70969366 MS.gene76722:CDS 45.0%
GAATCAGTCGTTGGAATTGG+TGG + chr4.1:70968300-70968319 MS.gene76722:CDS 45.0%
GCTACCGTTTCACCTTCAAA+TGG - chr4.1:70968544-70968563 None:intergenic 45.0%
GGCAATTCATGGTGTGGAAA+GGG + chr4.1:70969380-70969399 MS.gene76722:CDS 45.0%
GGTGTAATAGAGAATGGAGG+AGG - chr4.1:70967571-70967590 None:intergenic 45.0%
GGTTAGGTATTAACTCCGTG+TGG - chr4.1:70968474-70968493 None:intergenic 45.0%
GTAGCCGTTAAAGTTAACGG+TGG + chr4.1:70968559-70968578 MS.gene76722:CDS 45.0%
GTGACAATGTGTAGGAAAGG+TGG + chr4.1:70969333-70969352 MS.gene76722:CDS 45.0%
GTTCAAGACTTATCGGACAC+CGG - chr4.1:70968339-70968358 None:intergenic 45.0%
TCCTAAAACAGGTCAAACCC+TGG + chr4.1:70968447-70968466 MS.gene76722:CDS 45.0%
TGGTGTGGAAAGGGTGATTT+GGG + chr4.1:70969389-70969408 MS.gene76722:CDS 45.0%
TTCACAAAATCCGAGCCTAC+AGG + chr4.1:70968801-70968820 MS.gene76722:CDS 45.0%
TTGGCGAAAAATGGACCTGA+GGG + chr4.1:70969048-70969067 MS.gene76722:CDS 45.0%
! GACACGAATCGTTAGTGGAT+TGG + chr4.1:70969020-70969039 MS.gene76722:CDS 45.0%
! GAGGTGCGTTTTACGTATAG+TGG - chr4.1:70967663-70967682 None:intergenic 45.0%
! GGTGATTTGGGAGTTAATGG+CGG + chr4.1:70969401-70969420 MS.gene76722:CDS 45.0%
! GTTGAGGATTTTACGTCGGT+GGG + chr4.1:70969461-70969480 MS.gene76722:CDS 45.0%
! TGTTGAGGATTTTACGTCGG+TGG + chr4.1:70969460-70969479 MS.gene76722:CDS 45.0%
!! CGTTAGTGGATTGGTTGAGT+TGG + chr4.1:70969029-70969048 MS.gene76722:CDS 45.0%
!! CGTTCTTTTTGGTGCAACGA+CGG + chr4.1:70968246-70968265 MS.gene76722:CDS 45.0%
!! CGTTTATGAGCTTCGGGTTT+TGG + chr4.1:70968693-70968712 MS.gene76722:CDS 45.0%
!! TGGTGTCGTTTATGAGCTTC+GGG + chr4.1:70968687-70968706 MS.gene76722:CDS 45.0%
AACGACGCCGTTTCTGATGA+TGG - chr4.1:70967521-70967540 None:intergenic 50.0%
ACACCATGGAGCTGCGTTTA+AGG - chr4.1:70967612-70967631 None:intergenic 50.0%
AGGTGGATCAGAGATTGTGG+CGG + chr4.1:70969350-70969369 MS.gene76722:CDS 50.0%
ATTTGCTTTAGCCTCCCACG+TGG - chr4.1:70968938-70968957 None:intergenic 50.0%
CGACGCTTGTGACAATGTGT+AGG + chr4.1:70969325-70969344 MS.gene76722:CDS 50.0%
CGAGCTTGTATAGCTGAAGC+TGG + chr4.1:70968736-70968755 MS.gene76722:CDS 50.0%
CGGCAATTCATGGTGTGGAA+AGG + chr4.1:70969379-70969398 MS.gene76722:CDS 50.0%
CGGGAAATGATGGCCGTTTA+CGG + chr4.1:70968275-70968294 MS.gene76722:CDS 50.0%
CTTGAAGCAGTGGTTAAGCG+TGG + chr4.1:70969207-70969226 MS.gene76722:CDS 50.0%
GAAATGATGGCCGTTTACGG+CGG + chr4.1:70968278-70968297 MS.gene76722:CDS 50.0%
GATTTGGGAGTTAATGGCGG+TGG + chr4.1:70969404-70969423 MS.gene76722:CDS 50.0%
GCGGAATCAGTCGTTGGAAT+TGG + chr4.1:70968297-70968316 MS.gene76722:CDS 50.0%
GGAAGCAACAAGAATGACGG+CGG + chr4.1:70968603-70968622 MS.gene76722:CDS 50.0%
GGTGACGATACTTGAAGCAG+TGG + chr4.1:70969197-70969216 MS.gene76722:CDS 50.0%
GGTTAAGCGTGGAGGATTAG+TGG + chr4.1:70969218-70969237 MS.gene76722:CDS 50.0%
GTGGGAGGCTAAAGCAAATG+CGG + chr4.1:70968939-70968958 MS.gene76722:CDS 50.0%
GTTAAGGGAGGGATCAGAGA+GGG + chr4.1:70969284-70969303 MS.gene76722:CDS 50.0%
GTTCCTTAAACGCAGCTCCA+TGG + chr4.1:70967606-70967625 MS.gene76722:CDS 50.0%
GTTGGCGAAAAATGGACCTG+AGG + chr4.1:70969047-70969066 MS.gene76722:CDS 50.0%
TATTAACTCCGTGTGGGCCA+GGG - chr4.1:70968467-70968486 None:intergenic 50.0%
TGTTAAGGGAGGGATCAGAG+AGG + chr4.1:70969283-70969302 MS.gene76722:CDS 50.0%
TTTACGGCGGAATCAGTCGT+TGG + chr4.1:70968291-70968310 MS.gene76722:CDS 50.0%
TTTGCATCTCGCAGCGGATA+GGG + chr4.1:70969098-70969117 MS.gene76722:CDS 50.0%
TTTGGGAGTTAATGGCGGTG+GGG + chr4.1:70969406-70969425 MS.gene76722:CDS 50.0%
! ACACATTGTCACAAGCGTCG+CGG - chr4.1:70969325-70969344 None:intergenic 50.0%
! ACGACTGATTCCGCCGTAAA+CGG - chr4.1:70968291-70968310 None:intergenic 50.0%
! GCCAGGGTTTGACCTGTTTT+AGG - chr4.1:70968451-70968470 None:intergenic 50.0%
! GGCAATTTTGCATCTCGCAG+CGG + chr4.1:70969092-70969111 MS.gene76722:CDS 50.0%
! GGGTTTGGATGGTGGTGAAA+CGG + chr4.1:70969488-70969507 MS.gene76722:CDS 50.0%
! GTCGTTGGAATTGGTGGTTC+CGG + chr4.1:70968306-70968325 MS.gene76722:CDS 50.0%
! TAAGCGTGGAGGATTAGTGG+CGG + chr4.1:70969221-70969240 MS.gene76722:CDS 50.0%
! TTGGATGGTGGTGAAACGGA+GGG + chr4.1:70969492-70969511 MS.gene76722:CDS 50.0%
! TTTAAGATCGGGTGCCACGT+GGG + chr4.1:70968921-70968940 MS.gene76722:CDS 50.0%
! TTTGGATGGTGGTGAAACGG+AGG + chr4.1:70969491-70969510 MS.gene76722:CDS 50.0%
! TTTTGCATCTCGCAGCGGAT+AGG + chr4.1:70969097-70969116 MS.gene76722:CDS 50.0%
!! GCTTCGGGTTTTGGCGAAAT+CGG + chr4.1:70968702-70968721 MS.gene76722:CDS 50.0%
!! TGGGAAGGTTGGTAGAGAGT+GGG + chr4.1:70969127-70969146 MS.gene76722:CDS 50.0%
!!! TTTTAAGATCGGGTGCCACG+TGG + chr4.1:70968920-70968939 MS.gene76722:CDS 50.0%
AATCCCCGCTGTCATCTCCA+CGG - chr4.1:70969154-70969173 None:intergenic 55.0%
AGCGAAGAGGGACGCATTAG+CGG + chr4.1:70969071-70969090 MS.gene76722:CDS 55.0%
CATCGAAAACGACACCGAGG+AGG - chr4.1:70967682-70967701 None:intergenic 55.0%
GAAGCAGTGGTTAAGCGTGG+AGG + chr4.1:70969210-70969229 MS.gene76722:CDS 55.0%
GAGTGTTAGAGGGAGGAGGA+AGG + chr4.1:70969428-70969447 MS.gene76722:CDS 55.0%
GATGACAGCGGGGATTATGG+CGG + chr4.1:70969158-70969177 MS.gene76722:CDS 55.0%
GATTATGGCGGTAATGCCGG+AGG + chr4.1:70969170-70969189 MS.gene76722:CDS 55.0%
GCTTGCGGCAATTCATGGTG+TGG + chr4.1:70969374-70969393 MS.gene76722:CDS 55.0%
GGAGATGACAGCGGGGATTA+TGG + chr4.1:70969155-70969174 MS.gene76722:CDS 55.0%
GGAGGCTAAAGCAAATGCGG+CGG + chr4.1:70968942-70968961 MS.gene76722:CDS 55.0%
GGGGATTATGGCGGTAATGC+CGG + chr4.1:70969167-70969186 MS.gene76722:CDS 55.0%
GGTCATCGAAAACGACACCG+AGG - chr4.1:70967685-70967704 None:intergenic 55.0%
GTATTAACTCCGTGTGGGCC+AGG - chr4.1:70968468-70968487 None:intergenic 55.0%
TGTTAGAGGGAGGAGGAAGG+CGG + chr4.1:70969431-70969450 MS.gene76722:CDS 55.0%
! AGAGATTGTGGCGGAGCTTG+CGG + chr4.1:70969359-70969378 MS.gene76722:CDS 55.0%
! ATTGGGGACGGTGTTAAGGG+AGG + chr4.1:70969272-70969291 MS.gene76722:CDS 55.0%
! GAGGATTTTACGTCGGTGGG+CGG + chr4.1:70969464-70969483 MS.gene76722:CDS 55.0%
! GTTGGAATTGGTGGTTCCGG+CGG + chr4.1:70968309-70968328 MS.gene76722:CDS 55.0%
! TGGAGGATTAGTGGCGGTTG+CGG + chr4.1:70969227-70969246 MS.gene76722:CDS 55.0%
! TTGGGGACGGTGTTAAGGGA+GGG + chr4.1:70969273-70969292 MS.gene76722:CDS 55.0%
!! GACGCCGTTTCTGATGATGG+TGG - chr4.1:70967518-70967537 None:intergenic 55.0%
!! GGGAAGGTTGGTAGAGAGTG+GGG + chr4.1:70969128-70969147 MS.gene76722:CDS 55.0%
!! GTGGGAAGGTTGGTAGAGAG+TGG + chr4.1:70969126-70969145 MS.gene76722:CDS 55.0%
AGGTCAAACCCTGGCCCACA+CGG + chr4.1:70968456-70968475 MS.gene76722:CDS 60.0%
AGTATCGTCACCGCCTCCTC+CGG - chr4.1:70969189-70969208 None:intergenic 60.0%
ATCGGACACCGGTAATCCGC+CGG - chr4.1:70968328-70968347 None:intergenic 60.0%
ATGGACCTGAGGGAGCGAAG+AGG + chr4.1:70969058-70969077 MS.gene76722:CDS 60.0%
GATCCTGTCGTCGTGGCAAC+AGG + chr4.1:70968367-70968386 MS.gene76722:CDS 60.0%
GCGGAGCTTGCGGCAATTCA+TGG + chr4.1:70969369-70969388 MS.gene76722:CDS 60.0%
TAGGGAGACGGTGGGAAGGT+TGG + chr4.1:70969116-70969135 MS.gene76722:CDS 60.0%
TATGGCGGTAATGCCGGAGG+AGG + chr4.1:70969173-70969192 MS.gene76722:CDS 60.0%
TCTCGCAGCGGATAGGGAGA+CGG + chr4.1:70969104-70969123 MS.gene76722:CDS 60.0%
TGGACCTGAGGGAGCGAAGA+GGG + chr4.1:70969059-70969078 MS.gene76722:CDS 60.0%
TGTCCTGTTGCCACGACGAC+AGG - chr4.1:70968373-70968392 None:intergenic 60.0%
! ATTTTACGTCGGTGGGCGGC+GGG + chr4.1:70969468-70969487 MS.gene76722:CDS 60.0%
! GATTTTACGTCGGTGGGCGG+CGG + chr4.1:70969467-70969486 MS.gene76722:CDS 60.0%
!! ACGGCGTCGACGGGAAATGA+TGG + chr4.1:70968265-70968284 MS.gene76722:CDS 60.0%
!! ATGGCGGTGGGGAGTGTTAG+AGG + chr4.1:70969417-70969436 MS.gene76722:CDS 60.0%
!! TGGCGGTGGGGAGTGTTAGA+GGG + chr4.1:70969418-70969437 MS.gene76722:CDS 60.0%
!! TGGGGAGTGTTAGAGGGAGG+AGG + chr4.1:70969424-70969443 MS.gene76722:CDS 60.0%
!! TGGTGGTTCCGGCGGATTAC+CGG + chr4.1:70968317-70968336 MS.gene76722:CDS 60.0%
AGAGAGGGCGAGGGAGAGTG+CGG + chr4.1:70969299-70969318 MS.gene76722:CDS 65.0%
CGGATAGGGAGACGGTGGGA+AGG + chr4.1:70969112-70969131 MS.gene76722:CDS 65.0%
CGGTTGCGGCAGCGTTTGTG+GGG + chr4.1:70969241-70969260 MS.gene76722:CDS 65.0%
GATGCGGGATCCTGTCGTCG+TGG + chr4.1:70968360-70968379 MS.gene76722:CDS 65.0%
GCAGCGGATAGGGAGACGGT+GGG + chr4.1:70969108-70969127 MS.gene76722:CDS 65.0%
GCGGTTGCGGCAGCGTTTGT+GGG + chr4.1:70969240-70969259 MS.gene76722:CDS 65.0%
GGAAGGCGGCGACGTTGTTG+AGG + chr4.1:70969445-70969464 MS.gene76722:CDS 65.0%
GGAGGGATCAGAGAGGGCGA+GGG + chr4.1:70969290-70969309 MS.gene76722:CDS 65.0%
TGGGGCCGTGGAGATGACAG+CGG + chr4.1:70969146-70969165 MS.gene76722:CDS 65.0%
! AAGATCGGGTGCCACGTGGG+AGG + chr4.1:70968924-70968943 MS.gene76722:CDS 65.0%
! GTTGGTAGAGAGTGGGGCCG+TGG + chr4.1:70969134-70969153 MS.gene76722:CDS 65.0%
! TGGTGCAACGACGGCGTCGA+CGG + chr4.1:70968255-70968274 MS.gene76722:CDS 65.0%
!! CGGTGGGGAGTGTTAGAGGG+AGG + chr4.1:70969421-70969440 MS.gene76722:CDS 65.0%
CGCAGCGGATAGGGAGACGG+TGG + chr4.1:70969107-70969126 MS.gene76722:CDS 70.0%
GCGTCCCTCTTCGCTCCCTC+AGG - chr4.1:70969066-70969085 None:intergenic 70.0%
GGCGGTAATGCCGGAGGAGG+CGG + chr4.1:70969176-70969195 MS.gene76722:CDS 70.0%
GGCGGTTGCGGCAGCGTTTG+TGG + chr4.1:70969239-70969258 MS.gene76722:CDS 70.0%
GGGAGGGATCAGAGAGGGCG+AGG + chr4.1:70969289-70969308 MS.gene76722:CDS 70.0%
GGGCCGTGGAGATGACAGCG+GGG + chr4.1:70969148-70969167 MS.gene76722:CDS 70.0%
GGGGCCGTGGAGATGACAGC+GGG + chr4.1:70969147-70969166 MS.gene76722:CDS 70.0%
! TGGGCGGCGGGTTTGGATGG+TGG + chr4.1:70969480-70969499 MS.gene76722:CDS 70.0%
!! GGTGCAACGACGGCGTCGAC+GGG + chr4.1:70968256-70968275 MS.gene76722:CDS 70.0%
! ACGTCGGTGGGCGGCGGGTT+TGG + chr4.1:70969473-70969492 MS.gene76722:CDS 75.0%
! CGGTGGGCGGCGGGTTTGGA+TGG + chr4.1:70969477-70969496 MS.gene76722:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 70967479 70969590 70967479 ID=MS.gene76722
chr4.1 mRNA 70967479 70969590 70967479 ID=MS.gene76722.t1;Parent=MS.gene76722
chr4.1 exon 70967479 70969590 70967479 ID=MS.gene76722.t1.exon1;Parent=MS.gene76722.t1
chr4.1 CDS 70967479 70969590 70967479 ID=cds.MS.gene76722.t1;Parent=MS.gene76722.t1
Gene Sequence

>MS.gene76722

ATGGCAATATCACCTCAAACATTTCCAACTACTAAACCACCATCATCAGAAACGGCGTCGTTTATCACCCCAAATATCACTAACTTTAACCTCCTCCATTCTCTATTACACCTCACAAACCAAATATGTTCCTTAAACGCAGCTCCATGGTGTCGTTTCCTTTTAAACAAAATTTCCTCTTCCACTATACGTAAAACGCACCTCCTCGGTGTCGTTTTCGATGACCTAATTCGTAATTCAATATCAGTTTCAGCTTCGAATTCAACTCGTCAAAATTCTTACGTTCTTTGTTTAGAAGAAATGTATATTGTGCTTCATAGGATTAAAACTCTGCTCGAAGATTGTTCTAATGGAAGCAAATTTTCTATTCTTATGCAGATTGAAACTGTTGCTGATAATTTCAACAAATTAACAGGTGAATTATCAACTCTTCTTGATGTTTTTCCAATTCAAGAAATGAACTTGAATGATGATGTATTGGAGCTTGTTCTTCTGATTAGAAAACAGTGTTCAGAAACGAAGAATTTGTTAGGTGAAGAACAAATTCATCTCCGAAACGACGTTGTTTCTGTTCTAGAAGGAATCAAGAGAGAAATTGTTCCAAAAGATGAAAAACTATCTTCAATTTTTGAGAAATTGGAGATTCAAGATTCTTCAAGTTGTAGAAGTGAGATTGAAAATCTTCAAGAAGAAATTCAGAATCGGAGCGAAGAACAATCGAAATCAGAGATTGTTGCGTTGATCGGACTTGTTCGTTATGCGAAGTGCGTTCTTTTTGGTGCAACGACGGCGTCGACGGGAAATGATGGCCGTTTACGGCGGAATCAGTCGTTGGAATTGGTGGTTCCGGCGGATTACCGGTGTCCGATAAGTCTTGAACTGATGCGGGATCCTGTCGTCGTGGCAACAGGACAGACGTACGATCGTGTTTCGATTAAGCTTTGGATGGATTCTGGGCACAACACGTGTCCTAAAACAGGTCAAACCCTGGCCCACACGGAGTTAATACCTAACCATGCTTTGAGAAATATGATAGCTATTTGGTGCAAAGAACAGAAAATTCCATTTGAAGGTGAAACGGTAGCCGTTAAAGTTAACGGTGGTATGACAAATAAGACAGCTTTGGAAGCAACAAGAATGACGGCGGCGTTTTTAGTCAACAAGTTGACGTTGACTAACGGTTCTTTAACGTCGATGGAAGAGACAAATGGTGTCGTTTATGAGCTTCGGGTTTTGGCGAAATCGGATTCAGAAAGTCGAGCTTGTATAGCTGAAGCTGGAGCTATAGAGATTTTGGTTCGGTTTTTAAGTTACGAAGTTGGTTCACAAAATCCGAGCCTACAGGTTAATGCAGTTACAACGATTTTAAATCTCTCGATACTTGAAGCGAATAAGACAAGGATAATGGAGACTGAAGGTGTTTTAAGTGGTGTAGCAGAGGTTTTAAGATCGGGTGCCACGTGGGAGGCTAAAGCAAATGCGGCGGCTACTGTGTTTAGTTTAACTGGTGTGGTTGCTTTTAGAAAGCGGTTAGGTAGGAAGACACGAATCGTTAGTGGATTGGTTGAGTTGGCGAAAAATGGACCTGAGGGAGCGAAGAGGGACGCATTAGCGGCAATTTTGCATCTCGCAGCGGATAGGGAGACGGTGGGAAGGTTGGTAGAGAGTGGGGCCGTGGAGATGACAGCGGGGATTATGGCGGTAATGCCGGAGGAGGCGGTGACGATACTTGAAGCAGTGGTTAAGCGTGGAGGATTAGTGGCGGTTGCGGCAGCGTTTGTGGGGATAAAAAAATTGGGGACGGTGTTAAGGGAGGGATCAGAGAGGGCGAGGGAGAGTGCGGCCGCGACGCTTGTGACAATGTGTAGGAAAGGTGGATCAGAGATTGTGGCGGAGCTTGCGGCAATTCATGGTGTGGAAAGGGTGATTTGGGAGTTAATGGCGGTGGGGAGTGTTAGAGGGAGGAGGAAGGCGGCGACGTTGTTGAGGATTTTACGTCGGTGGGCGGCGGGTTTGGATGGTGGTGAAACGGAGGGATTTACATCAACGAACACTTTTGTTAGTACATCAACTACCATGGTTGTTCCAACTACAACTTCATTAGCACAGTAA
Protein sequence

>MS.gene76722.t1

MAISPQTFPTTKPPSSETASFITPNITNFNLLHSLLHLTNQICSLNAAPWCRFLLNKISSSTIRKTHLLGVVFDDLIRNSISVSASNSTRQNSYVLCLEEMYIVLHRIKTLLEDCSNGSKFSILMQIETVADNFNKLTGELSTLLDVFPIQEMNLNDDVLELVLLIRKQCSETKNLLGEEQIHLRNDVVSVLEGIKREIVPKDEKLSSIFEKLEIQDSSSCRSEIENLQEEIQNRSEEQSKSEIVALIGLVRYAKCVLFGATTASTGNDGRLRRNQSLELVVPADYRCPISLELMRDPVVVATGQTYDRVSIKLWMDSGHNTCPKTGQTLAHTELIPNHALRNMIAIWCKEQKIPFEGETVAVKVNGGMTNKTALEATRMTAAFLVNKLTLTNGSLTSMEETNGVVYELRVLAKSDSESRACIAEAGAIEILVRFLSYEVGSQNPSLQVNAVTTILNLSILEANKTRIMETEGVLSGVAEVLRSGATWEAKANAAATVFSLTGVVAFRKRLGRKTRIVSGLVELAKNGPEGAKRDALAAILHLAADRETVGRLVESGAVEMTAGIMAVMPEEAVTILEAVVKRGGLVAVAAAFVGIKKLGTVLREGSERARESAAATLVTMCRKGGSEIVAELAAIHGVERVIWELMAVGSVRGRRKAATLLRILRRWAAGLDGGETEGFTSTNTFVSTSTTMVVPTTTSLAQ