Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76802.t1 | XP_003613911.1 | 100 | 127 | 0 | 0 | 1 | 127 | 139 | 265 | 1.00E-62 | 249.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76802.t1 | G7JWJ7 | 100.0 | 127 | 0 | 0 | 1 | 127 | 139 | 265 | 7.3e-63 | 249.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049757 | MS.gene76802 | 0.828975 | 6.67E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene76802 | 0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene050553 | MS.gene76802 | 0.870853 | 1.07E-66 | -1.69E-46 |
| MS.gene051438 | MS.gene76802 | 0.806818 | 6.84E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene76802 | 0.816869 | 4.43E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene76802 | 0.806743 | 7.09E-50 | -1.69E-46 |
| MS.gene055159 | MS.gene76802 | -0.813824 | 2.10E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene76802 | 0.813231 | 2.84E-51 | -1.69E-46 |
| MS.gene055741 | MS.gene76802 | 0.825415 | 4.75E-54 | -1.69E-46 |
| MS.gene055744 | MS.gene76802 | 0.826234 | 3.03E-54 | -1.69E-46 |
| MS.gene056040 | MS.gene76802 | 0.806366 | 8.52E-50 | -1.69E-46 |
| MS.gene056508 | MS.gene76802 | 0.834857 | 2.35E-56 | -1.69E-46 |
| MS.gene059303 | MS.gene76802 | 0.812683 | 3.75E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene76802 | -0.802793 | 4.74E-49 | -1.69E-46 |
| MS.gene059667 | MS.gene76802 | 0.806327 | 8.68E-50 | -1.69E-46 |
| MS.gene059717 | MS.gene76802 | 0.827144 | 1.84E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene76802 | 0.806272 | 8.92E-50 | -1.69E-46 |
| MS.gene060372 | MS.gene76802 | 0.808584 | 2.88E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76802.t1 | MTR_5g042520 | 100.000 | 127 | 0 | 0 | 1 | 127 | 139 | 265 | 1.68e-88 | 258 |
| MS.gene76802.t1 | MTR_5g042520 | 100.000 | 54 | 0 | 0 | 1 | 54 | 139 | 192 | 9.98e-31 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76802.t1 | AT1G13990 | 70.312 | 128 | 29 | 2 | 1 | 125 | 154 | 275 | 1.30e-55 | 174 |
| MS.gene76802.t1 | AT1G13990 | 70.312 | 128 | 29 | 2 | 1 | 125 | 182 | 303 | 5.82e-55 | 174 |
| MS.gene76802.t1 | AT1G13990 | 70.248 | 121 | 27 | 2 | 8 | 125 | 192 | 306 | 2.86e-51 | 164 |
Find 36 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATTAAGCTTAAGGCTTT+CGG | 0.274485 | 5.2:+31787377 | None:intergenic |
| AGATTAAGCTTAAGGCTTTC+GGG | 0.343222 | 5.2:+31787378 | None:intergenic |
| GAGCTCGCAGATTAAGCTTA+AGG | 0.373495 | 5.2:+31787370 | None:intergenic |
| AATCACACCTTATATTTCTC+CGG | 0.387380 | 5.2:+31788239 | None:intergenic |
| TGAAAACCCGGAGAAATATA+AGG | 0.401206 | 5.2:-31788246 | MS.gene76802:intron |
| AGCTTAAGGCTTTCGGGTAC+AGG | 0.405348 | 5.2:+31787384 | None:intergenic |
| TCAGATCATCTTGATCTTCA+AGG | 0.424973 | 5.2:+31787337 | None:intergenic |
| CTAATATCTTCTACTTGCAT+TGG | 0.426258 | 5.2:+31788044 | None:intergenic |
| TTGATCTTTGCAAGAAAATG+AGG | 0.433526 | 5.2:-31787443 | MS.gene76802:CDS |
| TACCGATGAGGCGTCTATCT+CGG | 0.460916 | 5.2:+31787554 | None:intergenic |
| TTAATCTGCGAGCTCGAAAA+CGG | 0.468199 | 5.2:-31787362 | MS.gene76802:CDS |
| CAAGTAGAAGATATTAGAGA+TGG | 0.493706 | 5.2:-31788038 | MS.gene76802:CDS |
| AGCGAAGTTGAAAGAGAGCT+TGG | 0.516832 | 5.2:-31788146 | MS.gene76802:CDS |
| AAATCAGTCTAAACAAGCAA+GGG | 0.520422 | 5.2:-31788081 | MS.gene76802:CDS |
| AGATATTAGAGATGGAGTGC+TGG | 0.526832 | 5.2:-31788030 | MS.gene76802:intron |
| ATCACACCTTATATTTCTCC+GGG | 0.540391 | 5.2:+31788240 | None:intergenic |
| TGATCTTTGCAAGAAAATGA+GGG | 0.547590 | 5.2:-31787442 | MS.gene76802:CDS |
| AAAATCAGTCTAAACAAGCA+AGG | 0.561023 | 5.2:-31788082 | MS.gene76802:CDS |
| CAGATCATCTTGATCTTCAA+GGG | 0.562330 | 5.2:+31787338 | None:intergenic |
| CAGTTCTATTCCGCCGAGGT+AGG | 0.562349 | 5.2:-31787413 | MS.gene76802:CDS |
| CTTGCTGCAAGGAGGGTGTG+AGG | 0.562675 | 5.2:-31787583 | MS.gene76802:CDS |
| TTGCTGCAAGGAGGGTGTGA+GGG | 0.569430 | 5.2:-31787582 | MS.gene76802:CDS |
| CTTGGTTATTTGTACCGATG+AGG | 0.573244 | 5.2:+31787542 | None:intergenic |
| TGCCGAGATAGACGCCTCAT+CGG | 0.575782 | 5.2:-31787556 | MS.gene76802:intron |
| AGTCTAAACAAGCAAGGGGA+GGG | 0.591602 | 5.2:-31788076 | MS.gene76802:CDS |
| GTACAGGAGGTGTCCTACCT+CGG | 0.604704 | 5.2:+31787400 | None:intergenic |
| CAGTCTAAACAAGCAAGGGG+AGG | 0.606675 | 5.2:-31788077 | MS.gene76802:CDS |
| AATTGTGCACGATGAAAACC+CGG | 0.610498 | 5.2:-31788258 | None:intergenic |
| TTAAGGCTTTCGGGTACAGG+AGG | 0.618037 | 5.2:+31787387 | None:intergenic |
| AAGTATTGTGACTTGCTGCA+AGG | 0.620712 | 5.2:-31787594 | MS.gene76802:CDS |
| ATTGTGACTTGCTGCAAGGA+GGG | 0.645627 | 5.2:-31787590 | MS.gene76802:CDS |
| CTTGCAGTTCTATTCCGCCG+AGG | 0.660240 | 5.2:-31787417 | MS.gene76802:CDS |
| AATCAGTCTAAACAAGCAAG+GGG | 0.668776 | 5.2:-31788080 | MS.gene76802:CDS |
| CAGGAGGTGTCCTACCTCGG+CGG | 0.706198 | 5.2:+31787403 | None:intergenic |
| TATTGTGACTTGCTGCAAGG+AGG | 0.715644 | 5.2:-31787591 | MS.gene76802:CDS |
| GGTATTTGAAGATGAAAACG+AGG | 0.756977 | 5.2:-31787622 | MS.gene76802:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAATCATTTAACAATT+TGG | - | chr5.2:31787921-31787940 | MS.gene76802:intron | 10.0% |
| !! | ATTTCACATTACAAAATGTT+TGG | + | chr5.2:31787642-31787661 | None:intergenic | 20.0% |
| !! | TAGGAAAAAAAACAGAAAAA+AGG | + | chr5.2:31787368-31787387 | None:intergenic | 20.0% |
| !!! | AATATATCAGAAAGTATCAA+AGG | - | chr5.2:31787659-31787678 | MS.gene76802:intron | 20.0% |
| !!! | CTATCAATATTTGAAATCTT+CGG | - | chr5.2:31787385-31787404 | MS.gene76802:CDS | 20.0% |
| ! | ATTATGTTCAAACACATTAG+AGG | + | chr5.2:31787698-31787717 | None:intergenic | 25.0% |
| ! | ATTTAACAATTTGGCAATGT+TGG | - | chr5.2:31787930-31787949 | MS.gene76802:intron | 25.0% |
| ! | CGAAGATTTCAAATATTGAT+AGG | + | chr5.2:31787387-31787406 | None:intergenic | 25.0% |
| ! | CTAATGTGTTTGAACATAAT+TGG | - | chr5.2:31787698-31787717 | MS.gene76802:intron | 25.0% |
| ! | GTATACTAAGGAAAACTAAT+CGG | + | chr5.2:31787774-31787793 | None:intergenic | 25.0% |
| ! | TAATTGGAATTTGATTGCTT+TGG | - | chr5.2:31787714-31787733 | MS.gene76802:intron | 25.0% |
| ! | TATACTAAGGAAAACTAATC+GGG | + | chr5.2:31787773-31787792 | None:intergenic | 25.0% |
| AAAATCAGTCTAAACAAGCA+AGG | - | chr5.2:31787497-31787516 | MS.gene76802:intron | 30.0% | |
| AAATCAGTCTAAACAAGCAA+GGG | - | chr5.2:31787498-31787517 | MS.gene76802:intron | 30.0% | |
| AATCACACCTTATATTTCTC+CGG | + | chr5.2:31787343-31787362 | None:intergenic | 30.0% | |
| ATACTAAGGAAAACTAATCG+GGG | + | chr5.2:31787772-31787791 | None:intergenic | 30.0% | |
| ATAGTCAAAGTACAATTGCT+TGG | + | chr5.2:31788058-31788077 | None:intergenic | 30.0% | |
| CAAGTAGAAGATATTAGAGA+TGG | - | chr5.2:31787541-31787560 | MS.gene76802:intron | 30.0% | |
| CTAATATCTTCTACTTGCAT+TGG | + | chr5.2:31787538-31787557 | None:intergenic | 30.0% | |
| GAAGTTTATAAATTGCATCC+TGG | + | chr5.2:31787750-31787769 | None:intergenic | 30.0% | |
| GTATAAACCATGCAATATGT+TGG | + | chr5.2:31787875-31787894 | None:intergenic | 30.0% | |
| TGATCTTTGCAAGAAAATGA+GGG | - | chr5.2:31788137-31788156 | MS.gene76802:CDS | 30.0% | |
| TTAGAGTAGTAGGTATACTA+AGG | + | chr5.2:31787786-31787805 | None:intergenic | 30.0% | |
| TTGATCTTTGCAAGAAAATG+AGG | - | chr5.2:31788136-31788155 | MS.gene76802:CDS | 30.0% | |
| ! | AACTTACAGTTTAGAGTAGT+AGG | + | chr5.2:31787796-31787815 | None:intergenic | 30.0% |
| ! | CTAAACTGTAAGTTTGTTCA+TGG | - | chr5.2:31787802-31787821 | MS.gene76802:intron | 30.0% |
| ! | GTTTCATTTTACCAAGTTGT+GGG | + | chr5.2:31787838-31787857 | None:intergenic | 30.0% |
| ! | TGTTTCATTTTACCAAGTTG+TGG | + | chr5.2:31787839-31787858 | None:intergenic | 30.0% |
| !! | TGCTTCCTTTTGAAAACAAT+AGG | + | chr5.2:31787459-31787478 | None:intergenic | 30.0% |
| AATCAGTCTAAACAAGCAAG+GGG | - | chr5.2:31787499-31787518 | MS.gene76802:intron | 35.0% | |
| AGATTAAGCTTAAGGCTTTC+GGG | + | chr5.2:31788204-31788223 | None:intergenic | 35.0% | |
| ATCACACCTTATATTTCTCC+GGG | + | chr5.2:31787342-31787361 | None:intergenic | 35.0% | |
| CAGATCATCTTGATCTTCAA+GGG | + | chr5.2:31788244-31788263 | None:intergenic | 35.0% | |
| CAGATTAAGCTTAAGGCTTT+CGG | + | chr5.2:31788205-31788224 | None:intergenic | 35.0% | |
| GGTATTTGAAGATGAAAACG+AGG | - | chr5.2:31787957-31787976 | MS.gene76802:intron | 35.0% | |
| TCAGATCATCTTGATCTTCA+AGG | + | chr5.2:31788245-31788264 | None:intergenic | 35.0% | |
| TGAAAACCCGGAGAAATATA+AGG | - | chr5.2:31787333-31787352 | MS.gene76802:CDS | 35.0% | |
| ! | CTTGGCCTATTGTTTTCAAA+AGG | - | chr5.2:31787451-31787470 | MS.gene76802:intron | 35.0% |
| ! | GATTTTCTATTGCAGCTCTT+CGG | + | chr5.2:31787483-31787502 | None:intergenic | 35.0% |
| AGATATTAGAGATGGAGTGC+TGG | - | chr5.2:31787549-31787568 | MS.gene76802:intron | 40.0% | |
| TCATCGACCAACATATTGCA+TGG | - | chr5.2:31787865-31787884 | MS.gene76802:intron | 40.0% | |
| TGCTTTGGTAAATGCTAACC+AGG | - | chr5.2:31787729-31787748 | MS.gene76802:intron | 40.0% | |
| TTAATCTGCGAGCTCGAAAA+CGG | - | chr5.2:31788217-31788236 | MS.gene76802:intron | 40.0% | |
| TTACCAAGTTGTGGGTACTA+GGG | + | chr5.2:31787830-31787849 | None:intergenic | 40.0% | |
| TTTACCAAGTTGTGGGTACT+AGG | + | chr5.2:31787831-31787850 | None:intergenic | 40.0% | |
| ! | CTTGGTTATTTGTACCGATG+AGG | + | chr5.2:31788040-31788059 | None:intergenic | 40.0% |
| !! | AAGTATTGTGACTTGCTGCA+AGG | - | chr5.2:31787985-31788004 | MS.gene76802:intron | 40.0% |
| AGCGAAGTTGAAAGAGAGCT+TGG | - | chr5.2:31787433-31787452 | MS.gene76802:CDS | 45.0% | |
| AGTCTAAACAAGCAAGGGGA+GGG | - | chr5.2:31787503-31787522 | MS.gene76802:intron | 45.0% | |
| CAATTTGGCAATGTTGGTGC+AGG | - | chr5.2:31787936-31787955 | MS.gene76802:intron | 45.0% | |
| GAGCTCGCAGATTAAGCTTA+AGG | + | chr5.2:31788212-31788231 | None:intergenic | 45.0% | |
| !! | ATTGTGACTTGCTGCAAGGA+GGG | - | chr5.2:31787989-31788008 | MS.gene76802:intron | 45.0% |
| !! | TATTGTGACTTGCTGCAAGG+AGG | - | chr5.2:31787988-31788007 | MS.gene76802:intron | 45.0% |
| CAGTCTAAACAAGCAAGGGG+AGG | - | chr5.2:31787502-31787521 | MS.gene76802:intron | 50.0% | |
| GTACCCTAGTACCCACAACT+TGG | - | chr5.2:31787824-31787843 | MS.gene76802:intron | 50.0% | |
| ! | AGCTTAAGGCTTTCGGGTAC+AGG | + | chr5.2:31788198-31788217 | None:intergenic | 50.0% |
| ! | TACCGATGAGGCGTCTATCT+CGG | + | chr5.2:31788028-31788047 | None:intergenic | 50.0% |
| ! | TTAAGGCTTTCGGGTACAGG+AGG | + | chr5.2:31788195-31788214 | None:intergenic | 50.0% |
| CAGTTCTATTCCGCCGAGGT+AGG | - | chr5.2:31788166-31788185 | MS.gene76802:intron | 55.0% | |
| CTTGCAGTTCTATTCCGCCG+AGG | - | chr5.2:31788162-31788181 | MS.gene76802:intron | 55.0% | |
| TGCCGAGATAGACGCCTCAT+CGG | - | chr5.2:31788023-31788042 | MS.gene76802:intron | 55.0% | |
| ! | TTGCTGCAAGGAGGGTGTGA+GGG | - | chr5.2:31787997-31788016 | MS.gene76802:intron | 55.0% |
| !! | GTACAGGAGGTGTCCTACCT+CGG | + | chr5.2:31788182-31788201 | None:intergenic | 55.0% |
| ! | CTTGCTGCAAGGAGGGTGTG+AGG | - | chr5.2:31787996-31788015 | MS.gene76802:intron | 60.0% |
| !! | CAGGAGGTGTCCTACCTCGG+CGG | + | chr5.2:31788179-31788198 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 31787332 | 31788269 | 31787332 | ID=MS.gene76802 |
| chr5.2 | mRNA | 31787332 | 31788269 | 31787332 | ID=MS.gene76802.t1;Parent=MS.gene76802 |
| chr5.2 | exon | 31788247 | 31788269 | 31788247 | ID=MS.gene76802.t1.exon1;Parent=MS.gene76802.t1 |
| chr5.2 | CDS | 31788247 | 31788269 | 31788247 | ID=cds.MS.gene76802.t1;Parent=MS.gene76802.t1 |
| chr5.2 | exon | 31788031 | 31788166 | 31788031 | ID=MS.gene76802.t1.exon2;Parent=MS.gene76802.t1 |
| chr5.2 | CDS | 31788031 | 31788166 | 31788031 | ID=cds.MS.gene76802.t1;Parent=MS.gene76802.t1 |
| chr5.2 | exon | 31787557 | 31787643 | 31787557 | ID=MS.gene76802.t1.exon3;Parent=MS.gene76802.t1 |
| chr5.2 | CDS | 31787557 | 31787643 | 31787557 | ID=cds.MS.gene76802.t1;Parent=MS.gene76802.t1 |
| chr5.2 | exon | 31787332 | 31787469 | 31787332 | ID=MS.gene76802.t1.exon4;Parent=MS.gene76802.t1 |
| chr5.2 | CDS | 31787332 | 31787469 | 31787332 | ID=cds.MS.gene76802.t1;Parent=MS.gene76802.t1 |
| Gene Sequence |
| Protein sequence |
