AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77720.t1 MTR_4g035980 98.013 453 8 1 1 453 88 539 0.0 927
MS.gene77720.t1 MTR_3g008640 70.455 440 119 5 16 453 97 527 0.0 640
MS.gene77720.t1 MTR_3g008720 70.000 440 121 5 16 453 97 527 0.0 638
MS.gene77720.t1 MTR_3g033690 68.080 448 131 5 7 453 90 526 0.0 632
MS.gene77720.t1 MTR_3g008700 69.545 440 123 5 16 453 97 527 0.0 631
MS.gene77720.t1 MTR_7g053360 68.636 440 127 5 16 453 97 527 0.0 629
MS.gene77720.t1 MTR_4g025690 54.318 440 183 8 14 453 92 513 1.94e-161 466
MS.gene77720.t1 MTR_4g025730 52.154 441 188 9 14 453 91 509 3.55e-152 442
MS.gene77720.t1 MTR_8g104655 47.198 464 215 7 8 453 90 541 4.62e-149 435
MS.gene77720.t1 MTR_7g108905 49.213 445 204 8 20 449 115 552 7.00e-139 410
MS.gene77720.t1 MTR_8g104645 54.386 342 151 2 116 453 32 372 2.02e-135 394
MS.gene77720.t1 MTR_7g050980 47.248 436 222 5 21 453 125 555 3.12e-133 395
MS.gene77720.t1 MTR_4g025670 47.982 446 197 11 20 453 67 489 7.29e-133 392
MS.gene77720.t1 MTR_8g023310 46.121 464 226 8 11 453 129 589 1.74e-131 392
MS.gene77720.t1 MTR_7g050870 46.330 436 226 5 21 453 124 554 1.14e-129 386
MS.gene77720.t1 MTR_3g091380 45.833 456 219 8 21 453 147 597 9.74e-128 383
MS.gene77720.t1 MTR_4g087980 45.372 443 217 8 21 453 89 516 1.34e-125 374
MS.gene77720.t1 MTR_2g044880 47.901 405 201 6 50 453 130 525 9.58e-125 373
MS.gene77720.t1 MTR_1g008140 43.991 466 230 9 16 453 145 607 1.49e-124 375
MS.gene77720.t1 MTR_7g050950 43.908 435 235 6 22 453 125 553 2.51e-123 370
MS.gene77720.t1 MTR_8g104580 42.457 464 204 9 8 453 82 500 4.37e-121 362
MS.gene77720.t1 MTR_7g112580 43.764 457 230 9 11 453 99 542 8.49e-120 360
MS.gene77720.t1 MTR_7g108900 41.701 482 247 7 4 453 81 560 1.32e-118 358
MS.gene77720.t1 MTR_1g088020 42.437 476 241 7 9 453 82 555 2.55e-118 357
MS.gene77720.t1 MTR_1g054880 53.474 331 147 4 128 453 230 558 9.59e-117 353
MS.gene77720.t1 MTR_8g042910 43.923 469 210 12 20 453 162 612 9.61e-117 355
MS.gene77720.t1 MTR_2g044810 41.978 455 251 7 1 453 91 534 3.69e-116 351
MS.gene77720.t1 MTR_4g087970 42.506 447 228 8 21 453 86 517 9.40e-116 349
MS.gene77720.t1 MTR_1g083520 44.469 452 222 11 18 453 90 528 4.94e-115 348
MS.gene77720.t1 MTR_7g108890 51.964 331 152 4 128 453 234 562 2.34e-113 345
MS.gene77720.t1 MTR_3g010780 42.601 446 231 10 20 453 142 574 5.40e-113 344
MS.gene77720.t1 MTR_1g088225 41.807 476 242 8 9 453 82 553 9.44e-113 343
MS.gene77720.t1 MTR_4g124520 42.506 447 243 9 12 453 84 521 1.65e-112 341
MS.gene77720.t1 MTR_7g108875 41.505 465 238 9 20 453 103 564 2.42e-111 340
MS.gene77720.t1 MTR_3g010770 50.893 336 157 4 124 453 242 575 3.80e-111 339
MS.gene77720.t1 MTR_8g042900 43.439 442 231 10 21 453 150 581 4.03e-110 337
MS.gene77720.t1 MTR_0458s0030 58.123 277 112 3 179 453 40 314 4.59e-110 328
MS.gene77720.t1 MTR_1g088040 58.123 277 112 3 179 453 40 314 4.59e-110 328
MS.gene77720.t1 MTR_4g124550 39.738 458 256 8 12 453 140 593 9.96e-105 323
MS.gene77720.t1 MTR_0618s0020 60.526 228 89 1 226 453 2 228 2.38e-101 302
MS.gene77720.t1 MTR_8g023150 40.991 444 245 9 22 453 144 582 1.43e-100 312
MS.gene77720.t1 MTR_1g086210 43.503 354 191 4 107 453 218 569 4.23e-97 303
MS.gene77720.t1 MTR_1g083510 39.186 467 240 13 22 452 124 582 5.31e-97 303
MS.gene77720.t1 MTR_1g083470 39.659 411 229 8 52 449 162 566 1.42e-96 302
MS.gene77720.t1 MTR_1g083500 39.659 411 229 8 52 449 162 566 1.42e-96 302
MS.gene77720.t1 MTR_1g086190 37.792 471 261 10 7 453 101 563 5.40e-95 297
MS.gene77720.t1 MTR_1g083490 38.702 447 236 11 19 449 147 571 1.23e-94 297
MS.gene77720.t1 MTR_4g005170 38.255 447 238 11 19 449 147 571 4.65e-93 294
MS.gene77720.t1 MTR_7g112590 39.161 429 226 12 52 452 161 582 1.29e-92 292
MS.gene77720.t1 MTR_4g005180 37.360 447 242 10 19 449 129 553 2.06e-92 291
MS.gene77720.t1 MTR_4g120280 36.905 420 247 8 48 452 155 571 1.98e-90 286
MS.gene77720.t1 MTR_4g120290 36.105 421 249 9 48 452 155 571 7.68e-90 285
MS.gene77720.t1 MTR_2g436050 36.344 454 254 12 22 452 122 563 5.92e-89 281
MS.gene77720.t1 MTR_2g436040 35.141 461 277 12 1 450 94 543 6.34e-89 283
MS.gene77720.t1 MTR_2g044810 52.549 255 119 2 199 453 213 465 8.59e-89 278
MS.gene77720.t1 MTR_8g088610 41.141 333 191 3 119 450 45 373 6.29e-86 268
MS.gene77720.t1 MTR_2g044660 43.713 334 180 7 120 453 307 632 7.83e-86 275
MS.gene77720.t1 MTR_5g083620 39.481 347 198 5 113 453 16 356 7.66e-85 265
MS.gene77720.t1 MTR_7g110490 42.775 346 185 4 114 448 226 569 6.42e-84 269
MS.gene77720.t1 MTR_7g110510 42.775 346 185 4 114 448 226 569 6.42e-84 269
MS.gene77720.t1 MTR_8g093460 40.241 415 222 11 50 445 136 543 1.22e-83 268
MS.gene77720.t1 MTR_7g110440 37.644 433 239 8 50 453 144 574 5.00e-82 264
MS.gene77720.t1 MTR_7g110560 41.279 344 195 3 114 453 209 549 5.78e-82 263
MS.gene77720.t1 MTR_3g095920 37.288 472 245 15 13 452 111 563 3.42e-81 261
MS.gene77720.t1 MTR_2g105560 40.000 315 173 5 140 453 40 339 8.55e-75 238
MS.gene77720.t1 MTR_7g107180 39.557 316 177 5 139 453 5 307 2.60e-74 236
MS.gene77720.t1 MTR_7g110420 38.701 354 202 5 114 453 226 578 1.98e-71 236
MS.gene77720.t1 MTR_7g110430 35.328 351 180 5 114 453 226 540 2.64e-60 206
MS.gene77720.t1 MTR_0458s0010 31.381 478 254 11 9 453 82 518 4.26e-59 202
MS.gene77720.t1 MTR_7g111560 32.009 453 267 14 20 452 124 555 2.37e-58 201
MS.gene77720.t1 MTR_6g012800 36.140 285 159 5 141 415 6 277 5.40e-55 186
MS.gene77720.t1 MTR_6g088810 34.770 348 192 10 113 442 47 377 8.57e-53 182
MS.gene77720.t1 MTR_2g039530 36.218 312 181 8 112 420 23 319 1.29e-52 180
MS.gene77720.t1 MTR_1g086540 35.404 322 181 7 131 442 40 344 2.02e-52 180
MS.gene77720.t1 MTR_7g011530 37.762 286 149 7 143 415 8 277 6.49e-52 177
MS.gene77720.t1 MTR_6g024165 33.951 324 182 8 131 442 31 334 9.09e-52 178
MS.gene77720.t1 MTR_8g102380 35.505 307 168 9 149 442 88 377 1.93e-50 176
MS.gene77720.t1 MTR_7g092780 34.076 314 183 8 139 442 88 387 3.94e-45 162
MS.gene77720.t1 MTR_5g083590 30.380 316 167 5 139 453 5 268 2.58e-44 156
MS.gene77720.t1 MTR_7g102600 34.091 308 180 8 143 442 101 393 3.90e-43 156
MS.gene77720.t1 MTR_5g093970 31.908 304 188 6 141 442 43 329 1.33e-42 154
MS.gene77720.t1 MTR_4g124740 32.344 337 181 12 121 442 35 339 1.93e-42 153
MS.gene77720.t1 MTR_5g095490 34.416 308 173 8 147 442 67 357 3.39e-42 153
MS.gene77720.t1 MTR_8g102380 35.827 254 137 7 149 389 88 328 4.27e-41 149
MS.gene77720.t1 MTR_5g012600 32.278 316 187 8 141 445 63 362 4.39e-41 150
MS.gene77720.t1 MTR_6g042660 34.286 280 159 8 141 415 37 296 1.41e-39 145
MS.gene77720.t1 MTR_5g088300 33.443 305 175 11 141 442 37 316 9.58e-39 143
MS.gene77720.t1 MTR_5g088280 31.613 310 182 10 137 442 35 318 1.04e-36 137
MS.gene77720.t1 MTR_4g051810 33.696 276 162 7 141 415 37 292 2.74e-36 136
MS.gene77720.t1 MTR_2g033970 30.960 323 190 9 131 444 50 348 8.30e-36 135
MS.gene77720.t1 MTR_1g033050 32.333 300 176 9 124 415 4 284 9.77e-36 134
MS.gene77720.t1 MTR_6g042690 34.615 286 155 10 141 415 39 303 1.24e-35 135
MS.gene77720.t1 MTR_5g088290 33.935 277 161 7 141 415 48 304 5.95e-35 133
MS.gene77720.t1 MTR_4g074290 29.245 318 202 9 131 442 61 361 2.19e-33 129
MS.gene77720.t1 MTR_2g073390 29.653 317 202 8 131 442 61 361 6.15e-33 128
MS.gene77720.t1 MTR_8g036225 31.908 304 173 9 123 415 18 298 1.26e-32 126
MS.gene77720.t1 MTR_0009s0350 29.283 321 198 10 131 442 61 361 4.53e-31 123
MS.gene77720.t1 MTR_0009s0340 29.283 321 198 10 131 442 61 361 4.53e-31 123
MS.gene77720.t1 MTR_2g436600 30.061 326 176 12 133 444 51 338 1.15e-29 118
MS.gene77720.t1 MTR_6g025590 34.031 191 117 3 131 313 39 228 8.40e-28 111
MS.gene77720.t1 MTR_4g130790 32.727 165 97 5 170 325 53 212 5.01e-17 81.3
MS.gene77720.t1 MTR_0408s0030 38.776 98 50 2 318 415 36 123 6.46e-15 72.4
MS.gene77720.t1 MTR_2g436070 28.502 207 126 8 22 213 122 321 6.59e-14 73.6
MS.gene77720.t1 MTR_6g024200 32.203 177 91 6 270 442 125 276 7.16e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77720.t1 AT5G51490 60.526 456 168 6 4 453 87 536 0.0 567
MS.gene77720.t1 AT3G47400 61.504 452 165 4 9 453 145 594 0.0 565
MS.gene77720.t1 AT5G51500 61.712 444 163 5 14 453 100 540 0.0 565
MS.gene77720.t1 AT2G45220 53.515 441 183 11 14 453 92 511 2.20e-153 445
MS.gene77720.t1 AT1G23200 47.639 466 194 10 21 453 106 554 2.35e-136 403
MS.gene77720.t1 AT3G60730 56.918 318 134 2 138 453 203 519 1.27e-130 387
MS.gene77720.t1 AT1G11580 48.655 409 198 5 50 453 156 557 1.71e-128 383
MS.gene77720.t1 AT1G11580 48.655 409 198 5 50 453 177 578 3.88e-128 383
MS.gene77720.t1 AT4G00190 45.558 439 212 8 16 453 62 474 1.58e-127 378
MS.gene77720.t1 AT3G14310 44.654 477 227 9 11 453 119 592 1.80e-125 377
MS.gene77720.t1 AT1G53830 45.860 471 222 10 11 453 122 587 8.48e-125 375
MS.gene77720.t1 AT5G53370 46.341 451 226 8 16 453 138 585 1.81e-124 374
MS.gene77720.t1 AT5G53370 46.341 451 226 8 16 453 174 621 9.48e-124 373
MS.gene77720.t1 AT4G02320 45.824 443 226 8 21 453 80 518 2.00e-123 369
MS.gene77720.t1 AT3G49220 45.695 453 228 8 14 453 149 596 2.07e-123 372
MS.gene77720.t1 AT3G49220 45.695 453 228 8 14 453 194 641 8.87e-123 371
MS.gene77720.t1 AT3G59010 46.501 443 209 10 21 453 104 528 1.22e-121 365
MS.gene77720.t1 AT3G43270 44.519 447 230 7 21 453 83 525 1.79e-121 364
MS.gene77720.t1 AT4G33220 43.860 456 230 7 21 453 71 523 9.52e-119 357
MS.gene77720.t1 AT4G02300 42.070 454 245 8 11 453 86 532 4.79e-118 356
MS.gene77720.t1 AT4G33220 51.585 347 165 3 107 453 59 402 6.09e-118 351
MS.gene77720.t1 AT4G02330 41.598 488 241 9 8 453 86 571 2.69e-117 355
MS.gene77720.t1 AT5G04960 45.249 442 216 12 22 451 135 562 1.49e-116 353
MS.gene77720.t1 AT2G43050 45.000 440 214 9 20 453 99 516 6.27e-116 350
MS.gene77720.t1 AT3G05620 43.384 461 227 11 11 453 97 541 2.10e-115 349
MS.gene77720.t1 AT3G05610 40.899 445 245 8 22 453 126 565 8.27e-113 347
MS.gene77720.t1 AT5G27870 40.181 443 249 8 22 453 125 562 1.63e-111 345
MS.gene77720.t1 AT5G20860 41.319 455 246 10 7 453 97 538 1.90e-110 336
MS.gene77720.t1 AT1G02810 41.718 489 225 11 20 453 94 577 3.28e-110 337
MS.gene77720.t1 AT2G47550 43.939 462 227 10 20 453 101 558 6.21e-110 336
MS.gene77720.t1 AT3G10710 45.270 444 213 13 22 453 136 561 1.74e-109 335
MS.gene77720.t1 AT5G49180 42.029 414 222 8 50 449 157 566 2.59e-107 329
MS.gene77720.t1 AT3G10720 52.665 319 147 3 137 453 299 615 5.69e-107 330
MS.gene77720.t1 AT2G26450 41.176 408 230 7 50 453 210 611 8.17e-106 327
MS.gene77720.t1 AT4G33230 41.247 417 226 7 50 453 196 606 1.60e-104 323
MS.gene77720.t1 AT2G26440 41.037 463 246 8 8 453 95 547 3.78e-104 320
MS.gene77720.t1 AT3G06830 39.762 420 231 10 50 451 151 566 1.92e-103 319
MS.gene77720.t1 AT1G11590 43.980 407 216 7 50 453 127 524 6.64e-103 317
MS.gene77720.t1 AT3G27980 44.030 402 215 7 52 453 106 497 1.67e-102 315
MS.gene77720.t1 AT1G53840 40.733 464 231 10 13 447 133 581 1.67e-102 317
MS.gene77720.t1 AT4G03930 43.457 405 217 8 52 453 129 524 5.96e-102 314
MS.gene77720.t1 AT5G04970 49.216 319 158 3 137 453 304 620 1.57e-101 316
MS.gene77720.t1 AT3G10720 56.322 261 112 2 193 453 1 259 2.96e-100 301
MS.gene77720.t1 AT3G14300 40.177 453 242 11 16 448 523 966 3.89e-93 302
MS.gene77720.t1 AT4G15980 45.797 345 175 7 114 451 361 700 3.74e-89 286
MS.gene77720.t1 AT4G15980 45.797 345 175 7 114 451 371 710 5.80e-89 286
MS.gene77720.t1 AT5G20860 38.005 421 213 8 7 419 97 477 2.62e-87 276
MS.gene77720.t1 AT5G09760 45.171 321 151 7 136 441 236 546 2.46e-83 266
MS.gene77720.t1 AT5G09760 45.171 321 151 7 136 441 262 572 4.15e-83 267
MS.gene77720.t1 AT2G47040 41.620 358 190 8 109 453 244 595 1.13e-81 263
MS.gene77720.t1 AT1G11370 49.393 247 124 1 138 384 43 288 6.87e-81 252
MS.gene77720.t1 AT2G47030 40.111 359 197 6 109 453 234 588 1.89e-79 258
MS.gene77720.t1 AT5G64640 38.626 422 226 12 50 446 189 602 6.82e-77 251
MS.gene77720.t1 AT3G62170 37.602 367 203 7 109 453 226 588 5.06e-73 241
MS.gene77720.t1 AT2G36700 35.621 306 175 7 143 442 44 333 2.37e-51 177
MS.gene77720.t1 AT3G29090 36.396 283 157 6 143 415 9 278 2.69e-51 176
MS.gene77720.t1 AT5G19730 33.657 309 177 7 145 442 90 381 1.44e-49 173
MS.gene77720.t1 AT2G36710 32.602 319 192 8 138 447 86 390 1.37e-47 169
MS.gene77720.t1 AT2G21610 33.993 303 178 7 141 442 51 332 4.57e-47 166
MS.gene77720.t1 AT2G21610 33.993 303 178 7 141 442 56 337 6.96e-47 166
MS.gene77720.t1 AT1G05310 33.754 317 180 8 139 442 89 388 1.27e-45 163
MS.gene77720.t1 AT3G17060 32.609 322 190 9 130 443 35 337 3.90e-41 150
MS.gene77720.t1 AT5G07430 31.657 338 200 10 119 442 35 355 3.93e-41 150
MS.gene77720.t1 AT5G47500 31.629 313 187 7 141 442 61 357 4.36e-41 150
MS.gene77720.t1 AT1G44980 42.105 190 106 3 120 306 54 242 6.46e-38 138
MS.gene77720.t1 AT5G55590 32.133 361 211 12 96 442 38 378 1.25e-37 141
MS.gene77720.t1 AT3G24130 33.673 294 159 9 138 415 31 304 3.89e-37 139
MS.gene77720.t1 AT5G55590 33.553 304 172 10 96 385 38 325 4.98e-36 135
MS.gene77720.t1 AT5G61680 30.408 319 196 9 131 442 59 358 2.86e-34 132
MS.gene77720.t1 AT5G26810 31.973 294 162 9 141 415 34 308 2.56e-33 129
MS.gene77720.t1 AT5G18990 32.069 290 165 8 140 415 28 299 2.86e-33 128
MS.gene77720.t1 AT5G07420 29.499 339 206 11 119 442 35 355 1.11e-32 127
MS.gene77720.t1 AT2G19150 29.697 330 196 9 133 449 33 339 6.62e-32 125
MS.gene77720.t1 AT5G07410 29.321 324 197 11 110 415 22 331 1.91e-31 124
MS.gene77720.t1 AT1G69940 29.321 324 197 11 110 415 22 331 3.37e-31 123
MS.gene77720.t1 AT2G19150 31.203 266 151 7 133 385 33 279 2.50e-29 116
MS.gene77720.t1 AT2G47280 29.866 298 173 10 133 415 27 303 1.71e-23 101
MS.gene77720.t1 AT3G49220 32.287 223 134 7 14 223 194 412 1.25e-22 100

Find 116 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGGTACAATAAATTT+TGG 0.134887 4.4:-68298590 None:intergenic
ATGTTTGCTTGACCGTTTAA+AGG 0.191682 4.4:-68299481 None:intergenic
TTCTACCGTTGTGCCTTTAA+AGG 0.221697 4.4:+68299328 MS.gene77720:CDS
ATGTTTATAACTTTCATAAT+TGG 0.257834 4.4:-68298605 None:intergenic
CCGACAACGGGCTTGAAATC+TGG 0.267143 4.4:-68299580 None:intergenic
GTACAAGAATGATGCAGTTT+TGG 0.293323 4.4:-68299453 None:intergenic
GGAATCTTCGAGGGCTATTA+AGG 0.296557 4.4:+68298760 MS.gene77720:CDS
TATAGAGAATGTTACATCTA+TGG 0.296996 4.4:+68299391 MS.gene77720:CDS
GGCACCGTTGACTTCATCTT+TGG 0.305498 4.4:+68299412 MS.gene77720:CDS
GCACAAAGCACTCTATTCTA+TGG 0.307657 4.4:+68299724 MS.gene77720:CDS
CATCTTGTACTCTCTTAAAA+TGG 0.314188 4.4:-68298814 None:intergenic
CAATTCACAACTCTCAGATT+AGG 0.315294 4.4:+68299551 MS.gene77720:CDS
GGTGTCTTGGTAGCCTTTAA+AGG 0.325796 4.4:-68299341 None:intergenic
GCAATGGTTTATTCGTCAAT+TGG 0.333480 4.4:+68298642 MS.gene77720:CDS
AATTCACAACTCTCAGATTA+GGG 0.336514 4.4:+68299552 MS.gene77720:CDS
TTGTGTACTATAGGAATTGA+TGG 0.340716 4.4:+68299208 MS.gene77720:intron
ACCGTTGCTACCCTCCTTGC+TGG 0.365327 4.4:+68299844 MS.gene77720:CDS
TTGCCAGGGCCGGCCCAAAA+AGG 0.367122 4.4:-68299614 None:intergenic
CTATTTCTTGTTGATGATCC+AGG 0.370812 4.4:-68299766 None:intergenic
GTCCAATGGGGTGGTCTCCA+TGG 0.375821 4.4:+68299686 MS.gene77720:CDS
AACATCGAAACATGTCGAAA+TGG 0.379750 4.4:+68298548 MS.gene77720:CDS
CAATGGTTTATTCGTCAATT+GGG 0.384937 4.4:+68298643 MS.gene77720:CDS
GGTTCGAGAGAGGAAGCTAT+TGG 0.390413 4.4:+68298739 MS.gene77720:CDS
TCCAATGGGGTGGTCTCCAT+GGG 0.393186 4.4:+68299687 MS.gene77720:CDS
ACATGTTTCGATGTTTGTAT+AGG 0.395429 4.4:-68298540 None:intergenic
ACATCGAAACATGTCGAAAT+GGG 0.395879 4.4:+68298549 MS.gene77720:CDS
GTGTTGAATTTCCCGACAAC+GGG 0.400235 4.4:-68299592 None:intergenic
AAATGGGTCATCTCGACCTT+GGG 0.407983 4.4:-68299512 None:intergenic
GAAATCATTCATTGATAGCT+TGG 0.423252 4.4:+68299660 MS.gene77720:CDS
ATAATTTCGGACAAAATAGT+TGG 0.431028 4.4:+68298375 MS.gene77720:CDS
GATAGCTTGGTCAGTCCAAT+GGG 0.431876 4.4:+68299673 MS.gene77720:CDS
CATATCATTGTCGCGAAAGA+TGG 0.433386 4.4:+68298785 MS.gene77720:CDS
CTGAAGAAGGGTTTCCAAGA+TGG 0.434790 4.4:+68298711 MS.gene77720:CDS
AACTCTGGAGTATCTTTGCC+AGG 0.437542 4.4:-68299629 None:intergenic
TCTCGAACCGAAAACCATCT+TGG 0.449598 4.4:-68298725 None:intergenic
CATTGTCGCGAAAGATGGTT+CGG 0.450110 4.4:+68298790 MS.gene77720:CDS
CCAGATTTCAAGCCCGTTGT+CGG 0.455066 4.4:+68299580 MS.gene77720:CDS
TTAAACGTGATGGCCGTGTT+CGG 0.459371 4.4:+68298672 MS.gene77720:CDS
TATGGAGAGTACAAGAACTT+TGG 0.461429 4.4:+68299742 MS.gene77720:CDS
ACTGGTGTACCATTCACTTC+TGG 0.465852 4.4:+68299886 MS.gene77720:CDS
GAAATGGGTCATCTCGACCT+TGG 0.467978 4.4:-68299513 None:intergenic
AGAGTTGTGAATTGAAATTC+CGG 0.469872 4.4:-68299542 None:intergenic
CAGATTTCAAGCCCGTTGTC+GGG 0.471943 4.4:+68299581 MS.gene77720:CDS
ACCTGCAGTGGCAGAACTAT+AGG 0.476593 4.4:-68299014 None:intergenic
CTTGTACGAAGGCCTTTAAA+CGG 0.479605 4.4:+68299469 MS.gene77720:CDS
ATAGAGAGAATATTGTGGTT+GGG 0.482521 4.4:+68298903 MS.gene77720:CDS
CAAGATGCTATAAATGCTGC+TGG 0.484980 4.4:+68298830 MS.gene77720:CDS
AATATTATGTTGGTTGGTGA+TGG 0.489232 4.4:+68298938 MS.gene77720:CDS
TATAGAGAGAATATTGTGGT+TGG 0.491777 4.4:+68298902 MS.gene77720:CDS
TGATAGCTTGGTCAGTCCAA+TGG 0.494017 4.4:+68299672 MS.gene77720:CDS
CCCTCCTTGCTGGCACCACA+TGG 0.495961 4.4:+68299854 MS.gene77720:CDS
AGGAGGGTAGCAACGGTGAA+TGG 0.500791 4.4:-68299838 None:intergenic
ACCAACGATGTAACTGAAGA+AGG 0.504546 4.4:+68298698 MS.gene77720:CDS
AGAAATAGAGTAAGATGGCC+TGG 0.507966 4.4:+68299781 MS.gene77720:CDS
TGGAGTATCTTTGCCAGGGC+CGG 0.508397 4.4:-68299624 None:intergenic
AGTAATAGAAATGTTCAAGC+TGG 0.512268 4.4:+68298983 MS.gene77720:CDS
AATGATAATATTATGTTGGT+TGG 0.513930 4.4:+68298932 MS.gene77720:CDS
TAATTTCGGACAAAATAGTT+GGG 0.519031 4.4:+68298376 MS.gene77720:CDS
CCCCATGGAGACCACCCCAT+TGG 0.520496 4.4:-68299688 None:intergenic
TGATTTCATGACCACAACTC+TGG 0.520918 4.4:-68299644 None:intergenic
GATGAATGATAATATTATGT+TGG 0.524956 4.4:+68298928 MS.gene77720:CDS
ACCTATAGTTCTGCCACTGC+AGG 0.525895 4.4:+68299013 MS.gene77720:CDS
TAAATGCTGCTGGGAGGAGA+AGG 0.527517 4.4:+68298840 MS.gene77720:CDS
AACATGTCGAAATGGGGCAT+TGG 0.527557 4.4:+68298556 MS.gene77720:CDS
CCCTTCTTCAGTTACATCGT+TGG 0.528030 4.4:-68298699 None:intergenic
CCATGTGGTGCCAGCAAGGA+GGG 0.530412 4.4:-68299854 None:intergenic
ACCACATGGTTACCTGCCAC+TGG 0.533118 4.4:+68299868 MS.gene77720:CDS
GGAAGCTATTGGAATCTTCG+AGG 0.542630 4.4:+68298750 MS.gene77720:CDS
GTGAATGGTGATGCTTGAGC+TGG 0.544502 4.4:-68299823 None:intergenic
ATGGGGTGGTCTCCATGGGG+AGG 0.545885 4.4:+68299691 MS.gene77720:CDS
AAGAATATCATTACCTGCAG+TGG 0.552651 4.4:-68299026 None:intergenic
AATTAAAGGCCAGAAGTGAA+TGG 0.553145 4.4:-68299895 None:intergenic
CAACAAGAAATAGAGTAAGA+TGG 0.559275 4.4:+68299776 MS.gene77720:CDS
CCAACGATGTAACTGAAGAA+GGG 0.560246 4.4:+68298699 MS.gene77720:CDS
CGTGAACCATGAGGGTGTCT+TGG 0.562307 4.4:-68299354 None:intergenic
TGGGAATTCGTTAAACGTGA+TGG 0.567493 4.4:+68298662 MS.gene77720:CDS
GGTGTTGAATTTCCCGACAA+CGG 0.567667 4.4:-68299593 None:intergenic
GCCAGCAAGGAGGGTAGCAA+CGG 0.571084 4.4:-68299845 None:intergenic
GTGAATGGTACACCAGTGGC+AGG 0.575851 4.4:-68299880 None:intergenic
ATGACACAAATCATTAGCAA+TGG 0.576321 4.4:+68298626 MS.gene77720:CDS
ATTGTCGCGAAAGATGGTTC+GGG 0.578990 4.4:+68298791 MS.gene77720:CDS
CATCATTCGATGCACAAACA+TGG 0.581027 4.4:+68298507 MS.gene77720:CDS
CATCGTTGGTGTACCGAACA+CGG 0.586715 4.4:-68298685 None:intergenic
GCAAACATGATCACGGCCCA+AGG 0.588675 4.4:+68299496 MS.gene77720:CDS
TTTGTTATACGTGTGAAGAG+AGG 0.590375 4.4:+68298875 MS.gene77720:CDS
ACCACGTCGTGGTCAAGCAG+TGG 0.603947 4.4:+68299276 MS.gene77720:CDS
GAAGCTATTGGAATCTTCGA+GGG 0.606160 4.4:+68298751 MS.gene77720:CDS
ACTGCATCATTCTTGTACGA+AGG 0.614280 4.4:+68299458 MS.gene77720:CDS
GCTTGGTCAGTCCAATGGGG+TGG 0.614700 4.4:+68299677 MS.gene77720:CDS
TGCACAAACATGGCTAAGCA+CGG 0.617923 4.4:+68298517 MS.gene77720:CDS
TGTTTATAGAGAGAATATTG+TGG 0.617943 4.4:+68298898 MS.gene77720:CDS
CCAATGGGGTGGTCTCCATG+GGG 0.626856 4.4:+68299688 MS.gene77720:CDS
CATCGAAACATGTCGAAATG+GGG 0.628859 4.4:+68298550 MS.gene77720:CDS
GTAACCATGTGGTGCCAGCA+AGG 0.628912 4.4:-68299858 None:intergenic
GATGACCCATTTCAAAACAC+CGG 0.632618 4.4:+68299523 MS.gene77720:CDS
GCAAAGATACTCCAGAGTTG+TGG 0.635768 4.4:+68299633 MS.gene77720:CDS
CGGTCAAGCAAACATGATCA+CGG 0.638793 4.4:+68299489 MS.gene77720:CDS
AAGATGCTATAAATGCTGCT+GGG 0.644279 4.4:+68298831 MS.gene77720:CDS
GAGTACAAGAACTTTGGACC+TGG 0.646666 4.4:+68299748 MS.gene77720:CDS
GTTGCCAAAGATGAAGTCAA+CGG 0.646875 4.4:-68299416 None:intergenic
AGGCTACCAAGACACCCTCA+TGG 0.652925 4.4:+68299348 MS.gene77720:CDS
AACGGGCTTGAAATCTGGAG+CGG 0.657191 4.4:-68299575 None:intergenic
CACAACGGTAGAAAACAGAT+AGG 0.659204 4.4:-68299318 None:intergenic
ACTCTGGAGTATCTTTGCCA+GGG 0.660708 4.4:-68299628 None:intergenic
ACCATGTGGTGCCAGCAAGG+AGG 0.665765 4.4:-68299855 None:intergenic
GCAAAATTAGTACCTCCCCA+TGG 0.681295 4.4:-68299703 None:intergenic
AACACGGCTGGACCACGTCG+TGG 0.685767 4.4:+68299265 MS.gene77720:CDS
ATGCTATAAATGCTGCTGGG+AGG 0.686085 4.4:+68298834 MS.gene77720:CDS
AGAAGTGAATGGTACACCAG+TGG 0.693790 4.4:-68299884 None:intergenic
GCCACTGCTTGACCACGACG+TGG 0.699801 4.4:-68299277 None:intergenic
GACGTTGTGCGTGAACCATG+AGG 0.708175 4.4:-68299363 None:intergenic
GTAGAAAACAGATAGGTCAG+AGG 0.708320 4.4:-68299311 None:intergenic
ACGTTGTGCGTGAACCATGA+GGG 0.708924 4.4:-68299362 None:intergenic
GGTAGCCTTTAAAGGCACAA+CGG 0.723796 4.4:-68299333 None:intergenic
ACCAGTGGCAGGTAACCATG+TGG 0.747350 4.4:-68299869 None:intergenic
ATAGCTTGGTCAGTCCAATG+GGG 0.769302 4.4:+68299674 MS.gene77720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTATAACTTTCATAAT+TGG - chr4.4:68298608-68298627 None:intergenic 15.0%
!!! ATAATTGGTACAATAAATTT+TGG - chr4.4:68298593-68298612 None:intergenic 15.0%
!!! TAATTTTTTTTGTGTACTAT+AGG + chr4.4:68299199-68299218 MS.gene77720:intron 15.0%
!!! TCAACTAAAAAATTTTCTTT+AGG + chr4.4:68299075-68299094 MS.gene77720:intron 15.0%
!! AATGATAATATTATGTTGGT+TGG + chr4.4:68298932-68298951 MS.gene77720:CDS 20.0%
!! ATATATAACAATAAGGAAAG+AGG - chr4.4:68299133-68299152 None:intergenic 20.0%
!! GATGAATGATAATATTATGT+TGG + chr4.4:68298928-68298947 MS.gene77720:CDS 20.0%
!! GTTAGAGATATATAACAATA+AGG - chr4.4:68299140-68299159 None:intergenic 20.0%
! ATAATTTCGGACAAAATAGT+TGG + chr4.4:68298375-68298394 MS.gene77720:CDS 25.0%
! TAATTTCGGACAAAATAGTT+GGG + chr4.4:68298376-68298395 MS.gene77720:CDS 25.0%
! TATAGAGAATGTTACATCTA+TGG + chr4.4:68299391-68299410 MS.gene77720:CDS 25.0%
! TGTTTATAGAGAGAATATTG+TGG + chr4.4:68298898-68298917 MS.gene77720:CDS 25.0%
!!! ATGAACATGATTTTTTCTTG+TGG - chr4.4:68298490-68298509 None:intergenic 25.0%
!!! TGACATTACTTTTGAAAACA+CGG + chr4.4:68299249-68299268 MS.gene77720:CDS 25.0%
!!! TTCTTAGCTATTTTTCCTTA+TGG + chr4.4:68299044-68299063 MS.gene77720:intron 25.0%
AATTCACAACTCTCAGATTA+GGG + chr4.4:68299552-68299571 MS.gene77720:CDS 30.0%
ACATGTTTCGATGTTTGTAT+AGG - chr4.4:68298543-68298562 None:intergenic 30.0%
AGAGTTGTGAATTGAAATTC+CGG - chr4.4:68299545-68299564 None:intergenic 30.0%
AGTAATAGAAATGTTCAAGC+TGG + chr4.4:68298983-68299002 MS.gene77720:CDS 30.0%
ATAGAGAGAATATTGTGGTT+GGG + chr4.4:68298903-68298922 MS.gene77720:CDS 30.0%
ATGACACAAATCATTAGCAA+TGG + chr4.4:68298626-68298645 MS.gene77720:CDS 30.0%
CAACAAGAAATAGAGTAAGA+TGG + chr4.4:68299776-68299795 MS.gene77720:CDS 30.0%
CAATGGTTTATTCGTCAATT+GGG + chr4.4:68298643-68298662 MS.gene77720:CDS 30.0%
CATCTTGTACTCTCTTAAAA+TGG - chr4.4:68298817-68298836 None:intergenic 30.0%
TAGTTGAATTTGACTCCATA+AGG - chr4.4:68299062-68299081 None:intergenic 30.0%
TATAGAGAGAATATTGTGGT+TGG + chr4.4:68298902-68298921 MS.gene77720:CDS 30.0%
TTGTGTACTATAGGAATTGA+TGG + chr4.4:68299208-68299227 MS.gene77720:intron 30.0%
! CTTTTCATAACATGATAACC+AGG - chr4.4:68299802-68299821 None:intergenic 30.0%
! GAAATCATTCATTGATAGCT+TGG + chr4.4:68299660-68299679 MS.gene77720:CDS 30.0%
!! AATATTATGTTGGTTGGTGA+TGG + chr4.4:68298938-68298957 MS.gene77720:CDS 30.0%
!!! AAATTCCGGTGTTTTGAAAT+GGG - chr4.4:68299531-68299550 None:intergenic 30.0%
!!! AACATGATTTTTTCTTGTGG+AGG - chr4.4:68298487-68298506 None:intergenic 30.0%
!!! TGACACTGATTTTTTCTTCT+GGG - chr4.4:68298405-68298424 None:intergenic 30.0%
AACATCGAAACATGTCGAAA+TGG + chr4.4:68298548-68298567 MS.gene77720:CDS 35.0%
AAGAATATCATTACCTGCAG+TGG - chr4.4:68299029-68299048 None:intergenic 35.0%
AAGATGCTATAAATGCTGCT+GGG + chr4.4:68298831-68298850 MS.gene77720:CDS 35.0%
ACATCGAAACATGTCGAAAT+GGG + chr4.4:68298549-68298568 MS.gene77720:CDS 35.0%
ATGTTTGCTTGACCGTTTAA+AGG - chr4.4:68299484-68299503 None:intergenic 35.0%
CAATTCACAACTCTCAGATT+AGG + chr4.4:68299551-68299570 MS.gene77720:CDS 35.0%
CTAAAAATGTACGGTTGAGA+TGG - chr4.4:68298463-68298482 None:intergenic 35.0%
GCAATGGTTTATTCGTCAAT+TGG + chr4.4:68298642-68298661 MS.gene77720:CDS 35.0%
TGTGGAGGTCTAAAAATGTA+CGG - chr4.4:68298472-68298491 None:intergenic 35.0%
! GGGAAATTCAACACCTTTTT+GGG + chr4.4:68299601-68299620 MS.gene77720:CDS 35.0%
! GTACAAGAATGATGCAGTTT+TGG - chr4.4:68299456-68299475 None:intergenic 35.0%
! TATGGAGAGTACAAGAACTT+TGG + chr4.4:68299742-68299761 MS.gene77720:CDS 35.0%
! TTTGTTATACGTGTGAAGAG+AGG + chr4.4:68298875-68298894 MS.gene77720:CDS 35.0%
!! ATTACTTTTGAAAACACGGC+TGG + chr4.4:68299253-68299272 MS.gene77720:CDS 35.0%
!! CTATTTCTTGTTGATGATCC+AGG - chr4.4:68299769-68299788 None:intergenic 35.0%
!!! GAAATTCCGGTGTTTTGAAA+TGG - chr4.4:68299532-68299551 None:intergenic 35.0%
!!! GATGCAGTTTTGGAAAACTA+CGG - chr4.4:68299446-68299465 None:intergenic 35.0%
!!! GTGACACTGATTTTTTCTTC+TGG - chr4.4:68298406-68298425 None:intergenic 35.0%
ACCAACGATGTAACTGAAGA+AGG + chr4.4:68298698-68298717 MS.gene77720:CDS 40.0%
ACTGCATCATTCTTGTACGA+AGG + chr4.4:68299458-68299477 MS.gene77720:CDS 40.0%
AGAAATAGAGTAAGATGGCC+TGG + chr4.4:68299781-68299800 MS.gene77720:CDS 40.0%
CAAGATGCTATAAATGCTGC+TGG + chr4.4:68298830-68298849 MS.gene77720:CDS 40.0%
CACAACGGTAGAAAACAGAT+AGG - chr4.4:68299321-68299340 None:intergenic 40.0%
CATATCATTGTCGCGAAAGA+TGG + chr4.4:68298785-68298804 MS.gene77720:CDS 40.0%
CATCATTCGATGCACAAACA+TGG + chr4.4:68298507-68298526 MS.gene77720:CDS 40.0%
CATCGAAACATGTCGAAATG+GGG + chr4.4:68298550-68298569 MS.gene77720:CDS 40.0%
CCAACGATGTAACTGAAGAA+GGG + chr4.4:68298699-68298718 MS.gene77720:CDS 40.0%
CTTGTACGAAGGCCTTTAAA+CGG + chr4.4:68299469-68299488 MS.gene77720:CDS 40.0%
GAAGCTATTGGAATCTTCGA+GGG + chr4.4:68298751-68298770 MS.gene77720:CDS 40.0%
GATGACCCATTTCAAAACAC+CGG + chr4.4:68299523-68299542 MS.gene77720:CDS 40.0%
GTAGAAAACAGATAGGTCAG+AGG - chr4.4:68299314-68299333 None:intergenic 40.0%
GTTGCCAAAGATGAAGTCAA+CGG - chr4.4:68299419-68299438 None:intergenic 40.0%
TGATTTCATGACCACAACTC+TGG - chr4.4:68299647-68299666 None:intergenic 40.0%
TGGGAATTCGTTAAACGTGA+TGG + chr4.4:68298662-68298681 MS.gene77720:CDS 40.0%
TTCTACCGTTGTGCCTTTAA+AGG + chr4.4:68299328-68299347 MS.gene77720:CDS 40.0%
! AGGGTTTCCAAGATGGTTTT+CGG + chr4.4:68298718-68298737 MS.gene77720:CDS 40.0%
! CGGGAAATTCAACACCTTTT+TGG + chr4.4:68299600-68299619 MS.gene77720:CDS 40.0%
! GCACAAAGCACTCTATTCTA+TGG + chr4.4:68299724-68299743 MS.gene77720:CDS 40.0%
!! AATTCAACACCTTTTTGGGC+CGG + chr4.4:68299605-68299624 MS.gene77720:CDS 40.0%
AAATGGGTCATCTCGACCTT+GGG - chr4.4:68299515-68299534 None:intergenic 45.0%
AACATGTCGAAATGGGGCAT+TGG + chr4.4:68298556-68298575 MS.gene77720:CDS 45.0%
ACTGGTGTACCATTCACTTC+TGG + chr4.4:68299886-68299905 MS.gene77720:CDS 45.0%
AGAAGTGAATGGTACACCAG+TGG - chr4.4:68299887-68299906 None:intergenic 45.0%
ATAGCTTGGTCAGTCCAATG+GGG + chr4.4:68299674-68299693 MS.gene77720:CDS 45.0%
ATGCTATAAATGCTGCTGGG+AGG + chr4.4:68298834-68298853 MS.gene77720:CDS 45.0%
ATTGTCGCGAAAGATGGTTC+GGG + chr4.4:68298791-68298810 MS.gene77720:CDS 45.0%
CATTGTCGCGAAAGATGGTT+CGG + chr4.4:68298790-68298809 MS.gene77720:CDS 45.0%
CCCTTCTTCAGTTACATCGT+TGG - chr4.4:68298702-68298721 None:intergenic 45.0%
CGGTCAAGCAAACATGATCA+CGG + chr4.4:68299489-68299508 MS.gene77720:CDS 45.0%
CTGAAGAAGGGTTTCCAAGA+TGG + chr4.4:68298711-68298730 MS.gene77720:CDS 45.0%
GAGTACAAGAACTTTGGACC+TGG + chr4.4:68299748-68299767 MS.gene77720:CDS 45.0%
GATAGCTTGGTCAGTCCAAT+GGG + chr4.4:68299673-68299692 MS.gene77720:CDS 45.0%
GCAAAATTAGTACCTCCCCA+TGG - chr4.4:68299706-68299725 None:intergenic 45.0%
GCAAAGATACTCCAGAGTTG+TGG + chr4.4:68299633-68299652 MS.gene77720:CDS 45.0%
GGAAGCTATTGGAATCTTCG+AGG + chr4.4:68298750-68298769 MS.gene77720:CDS 45.0%
GGAATCTTCGAGGGCTATTA+AGG + chr4.4:68298760-68298779 MS.gene77720:CDS 45.0%
GTGTTGAATTTCCCGACAAC+GGG - chr4.4:68299595-68299614 None:intergenic 45.0%
TCTCGAACCGAAAACCATCT+TGG - chr4.4:68298728-68298747 None:intergenic 45.0%
TGATAGCTTGGTCAGTCCAA+TGG + chr4.4:68299672-68299691 MS.gene77720:CDS 45.0%
TGCACAAACATGGCTAAGCA+CGG + chr4.4:68298517-68298536 MS.gene77720:CDS 45.0%
TTAAACGTGATGGCCGTGTT+CGG + chr4.4:68298672-68298691 MS.gene77720:CDS 45.0%
! GGTAGCCTTTAAAGGCACAA+CGG - chr4.4:68299336-68299355 None:intergenic 45.0%
! GGTGTCTTGGTAGCCTTTAA+AGG - chr4.4:68299344-68299363 None:intergenic 45.0%
! GGTGTTGAATTTCCCGACAA+CGG - chr4.4:68299596-68299615 None:intergenic 45.0%
!! AACTCTGGAGTATCTTTGCC+AGG - chr4.4:68299632-68299651 None:intergenic 45.0%
!! ACTCTGGAGTATCTTTGCCA+GGG - chr4.4:68299631-68299650 None:intergenic 45.0%
ACCTGCAGTGGCAGAACTAT+AGG - chr4.4:68299017-68299036 None:intergenic 50.0%
ACGTTGTGCGTGAACCATGA+GGG - chr4.4:68299365-68299384 None:intergenic 50.0%
CAGATTTCAAGCCCGTTGTC+GGG + chr4.4:68299581-68299600 MS.gene77720:CDS 50.0%
CATCGTTGGTGTACCGAACA+CGG - chr4.4:68298688-68298707 None:intergenic 50.0%
CCAGATTTCAAGCCCGTTGT+CGG + chr4.4:68299580-68299599 MS.gene77720:CDS 50.0%
GAAATGGGTCATCTCGACCT+TGG - chr4.4:68299516-68299535 None:intergenic 50.0%
GGTTCGAGAGAGGAAGCTAT+TGG + chr4.4:68298739-68298758 MS.gene77720:CDS 50.0%
TAAATGCTGCTGGGAGGAGA+AGG + chr4.4:68298840-68298859 MS.gene77720:CDS 50.0%
! AACGGGCTTGAAATCTGGAG+CGG - chr4.4:68299578-68299597 None:intergenic 50.0%
! ACCTATAGTTCTGCCACTGC+AGG + chr4.4:68299013-68299032 MS.gene77720:CDS 50.0%
! GTGAATGGTGATGCTTGAGC+TGG - chr4.4:68299826-68299845 None:intergenic 50.0%
!! GATGGTTTTCGGTTCGAGAG+AGG + chr4.4:68298729-68298748 MS.gene77720:CDS 50.0%
!! GGCACCGTTGACTTCATCTT+TGG + chr4.4:68299412-68299431 MS.gene77720:CDS 50.0%
ACCACATGGTTACCTGCCAC+TGG + chr4.4:68299868-68299887 MS.gene77720:CDS 55.0%
ACCAGTGGCAGGTAACCATG+TGG - chr4.4:68299872-68299891 None:intergenic 55.0%
AGGAGGGTAGCAACGGTGAA+TGG - chr4.4:68299841-68299860 None:intergenic 55.0%
AGGCTACCAAGACACCCTCA+TGG + chr4.4:68299348-68299367 MS.gene77720:CDS 55.0%
GACGTTGTGCGTGAACCATG+AGG - chr4.4:68299366-68299385 None:intergenic 55.0%
GCAAACATGATCACGGCCCA+AGG + chr4.4:68299496-68299515 MS.gene77720:CDS 55.0%
GTGAATGGTACACCAGTGGC+AGG - chr4.4:68299883-68299902 None:intergenic 55.0%
TCCAATGGGGTGGTCTCCAT+GGG + chr4.4:68299687-68299706 MS.gene77720:CDS 55.0%
! CCGACAACGGGCTTGAAATC+TGG - chr4.4:68299583-68299602 None:intergenic 55.0%
! GTAACCATGTGGTGCCAGCA+AGG - chr4.4:68299861-68299880 None:intergenic 55.0%
! TGGAGTATCTTTGCCAGGGC+CGG - chr4.4:68299627-68299646 None:intergenic 55.0%
!! CGTGAACCATGAGGGTGTCT+TGG - chr4.4:68299357-68299376 None:intergenic 55.0%
ACCACGTCGTGGTCAAGCAG+TGG + chr4.4:68299276-68299295 MS.gene77720:CDS 60.0%
ACCGTTGCTACCCTCCTTGC+TGG + chr4.4:68299844-68299863 MS.gene77720:CDS 60.0%
CCAATGGGGTGGTCTCCATG+GGG + chr4.4:68299688-68299707 MS.gene77720:CDS 60.0%
GCCAGCAAGGAGGGTAGCAA+CGG - chr4.4:68299848-68299867 None:intergenic 60.0%
GCTTGGTCAGTCCAATGGGG+TGG + chr4.4:68299677-68299696 MS.gene77720:CDS 60.0%
GTCCAATGGGGTGGTCTCCA+TGG + chr4.4:68299686-68299705 MS.gene77720:CDS 60.0%
! ACCATGTGGTGCCAGCAAGG+AGG - chr4.4:68299858-68299877 None:intergenic 60.0%
! CCATGTGGTGCCAGCAAGGA+GGG - chr4.4:68299857-68299876 None:intergenic 60.0%
AACACGGCTGGACCACGTCG+TGG + chr4.4:68299265-68299284 MS.gene77720:CDS 65.0%
ATGGGGTGGTCTCCATGGGG+AGG + chr4.4:68299691-68299710 MS.gene77720:CDS 65.0%
CCCCATGGAGACCACCCCAT+TGG - chr4.4:68299691-68299710 None:intergenic 65.0%
CCCTCCTTGCTGGCACCACA+TGG + chr4.4:68299854-68299873 MS.gene77720:CDS 65.0%
GCCACTGCTTGACCACGACG+TGG - chr4.4:68299280-68299299 None:intergenic 65.0%
TTGCCAGGGCCGGCCCAAAA+AGG - chr4.4:68299617-68299636 None:intergenic 65.0%
!! ACACCTTTTTGGGCCGGCCC+TGG + chr4.4:68299611-68299630 MS.gene77720:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 68298368 68299915 68298368 ID=MS.gene77720
chr4.4 mRNA 68298368 68299915 68298368 ID=MS.gene77720.t1;Parent=MS.gene77720
chr4.4 exon 68298368 68299034 68298368 ID=MS.gene77720.t1.exon1;Parent=MS.gene77720.t1
chr4.4 CDS 68298368 68299034 68298368 ID=cds.MS.gene77720.t1;Parent=MS.gene77720.t1
chr4.4 exon 68299221 68299915 68299221 ID=MS.gene77720.t1.exon2;Parent=MS.gene77720.t1
chr4.4 CDS 68299221 68299915 68299221 ID=cds.MS.gene77720.t1;Parent=MS.gene77720.t1
Gene Sequence

>MS.gene77720

ATGGCGCATAATTTCGGACAAAATAGTTGGGTCACCCAGAAGAAAAAATCAGTGTCACGTGATTGTTTAAAACTCTTCGACAACACAGTTTTCCATCTCAACCGTACATTTTTAGACCTCCACAAGAAAAAATCATGTTCATCATTCGATGCACAAACATGGCTAAGCACGGCCTATACAAACATCGAAACATGTCGAAATGGGGCATTGGAATTGAACATTCCAAAATTTATTGTACCAATTATGAAAGTTATAAACATGACACAAATCATTAGCAATGGTTTATTCGTCAATTGGGAATTCGTTAAACGTGATGGCCGTGTTCGGTACACCAACGATGTAACTGAAGAAGGGTTTCCAAGATGGTTTTCGGTTCGAGAGAGGAAGCTATTGGAATCTTCGAGGGCTATTAAGGCGCATATCATTGTCGCGAAAGATGGTTCGGGCCATTTTAAGAGAGTACAAGATGCTATAAATGCTGCTGGGAGGAGAAGGTTTAAAACAAGATTTGTTATACGTGTGAAGAGAGGTGTTTATAGAGAGAATATTGTGGTTGGGAAGATGAATGATAATATTATGTTGGTTGGTGATGGAAGTAGATATACAGTGATAACAAGTAATAGAAATGTTCAAGCTGGTTTTACTACCTATAGTTCTGCCACTGCAGGTAATGATATTCTTAGCTATTTTTCCTTATGGAGTCAAATTCAACTAAAAAATTTTCTTTAGGAATATGAATTCTATAATTCTATTCATTCTTCTCCTCTTTCCTTATTGTTATATATCTCTAACTATTATTATCATAACAAATTCAAATCAACACATTCTAAATAATTTTTTTTGTGTACTATAGGAATTGATGGTCTTCACTTCATTGCACGTGACATTACTTTTGAAAACACGGCTGGACCACGTCGTGGTCAAGCAGTGGCGCTTCGATCAGCCTCTGACCTATCTGTTTTCTACCGTTGTGCCTTTAAAGGCTACCAAGACACCCTCATGGTTCACGCACAACGTCAATTTTATAGAGAATGTTACATCTATGGCACCGTTGACTTCATCTTTGGCAACGCAGCCGTAGTTTTCCAAAACTGCATCATTCTTGTACGAAGGCCTTTAAACGGTCAAGCAAACATGATCACGGCCCAAGGTCGAGATGACCCATTTCAAAACACCGGAATTTCAATTCACAACTCTCAGATTAGGGCCGCTCCAGATTTCAAGCCCGTTGTCGGGAAATTCAACACCTTTTTGGGCCGGCCCTGGCAAAGATACTCCAGAGTTGTGGTCATGAAATCATTCATTGATAGCTTGGTCAGTCCAATGGGGTGGTCTCCATGGGGAGGTACTAATTTTGCACAAAGCACTCTATTCTATGGAGAGTACAAGAACTTTGGACCTGGATCATCAACAAGAAATAGAGTAAGATGGCCTGGTTATCATGTTATGAAAAGTCCAGCTCAAGCATCACCATTCACCGTTGCTACCCTCCTTGCTGGCACCACATGGTTACCTGCCACTGGTGTACCATTCACTTCTGGCCTTTAA
Protein sequence

>MS.gene77720.t1

MAHNFGQNSWVTQKKKSVSRDCLKLFDNTVFHLNRTFLDLHKKKSCSSFDAQTWLSTAYTNIETCRNGALELNIPKFIVPIMKVINMTQIISNGLFVNWEFVKRDGRVRYTNDVTEEGFPRWFSVRERKLLESSRAIKAHIIVAKDGSGHFKRVQDAINAAGRRRFKTRFVIRVKRGVYRENIVVGKMNDNIMLVGDGSRYTVITSNRNVQAGFTTYSSATAGIDGLHFIARDITFENTAGPRRGQAVALRSASDLSVFYRCAFKGYQDTLMVHAQRQFYRECYIYGTVDFIFGNAAVVFQNCIILVRRPLNGQANMITAQGRDDPFQNTGISIHNSQIRAAPDFKPVVGKFNTFLGRPWQRYSRVVVMKSFIDSLVSPMGWSPWGGTNFAQSTLFYGEYKNFGPGSSTRNRVRWPGYHVMKSPAQASPFTVATLLAGTTWLPATGVPFTSGL