Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78240.t1 | XP_013448803.1 | 96.4 | 84 | 3 | 0 | 2 | 85 | 91 | 174 | 3.00E-34 | 154.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78240.t1 | A0A072UAI2 | 96.4 | 84 | 3 | 0 | 2 | 85 | 91 | 174 | 2.1e-34 | 154.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049609 | MS.gene78240 | 0.824001 | 1.02E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene78240 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene050593 | MS.gene78240 | 0.803613 | 3.21E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene78240 | 0.804949 | 1.69E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene78240 | 0.818044 | 2.41E-52 | -1.69E-46 |
| MS.gene052346 | MS.gene78240 | 0.813385 | 2.63E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene78240 | 0.813966 | 1.96E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene78240 | 0.809011 | 2.34E-50 | -1.69E-46 |
| MS.gene053436 | MS.gene78240 | 0.826324 | 2.89E-54 | -1.69E-46 |
| MS.gene053574 | MS.gene78240 | 0.805587 | 1.24E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene78240 | 0.817457 | 3.27E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene78240 | 0.825036 | 5.83E-54 | -1.69E-46 |
| MS.gene059718 | MS.gene78240 | 0.801086 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78240.t1 | MTR_7g059255 | 96.429 | 84 | 3 | 0 | 2 | 85 | 91 | 174 | 5.95e-54 | 165 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78240.t1 | AT4G13220 | 59.302 | 86 | 33 | 1 | 2 | 85 | 91 | 176 | 2.54e-29 | 103 |
Find 22 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTACAGCTTTCTTTATTT+TGG | 0.099548 | 7.4:+44190490 | MS.gene78240:CDS |
| GTTACTGAAATCCTTGCTAT+TGG | 0.328579 | 7.4:-44190422 | None:intergenic |
| ATCCTTTGGAAGAGGCTAAT+AGG | 0.388720 | 7.4:+44191338 | MS.gene78240:CDS |
| CTTGATCTTTATTGAATGCT+AGG | 0.393502 | 7.4:-44191270 | None:intergenic |
| GAAATCCTTGCTATTGGTCT+TGG | 0.422289 | 7.4:-44190416 | None:intergenic |
| CAAAGGATCATCCATAGGTT+TGG | 0.442636 | 7.4:-44191323 | None:intergenic |
| CAAACCTATGGATGATCCTT+TGG | 0.442745 | 7.4:+44191324 | MS.gene78240:CDS |
| GGAAGAGGCTAATAGGATAA+TGG | 0.446378 | 7.4:+44191345 | MS.gene78240:CDS |
| CAAGAACCACCTAGCGGGAA+TGG | 0.467436 | 7.4:+44191289 | MS.gene78240:CDS |
| GTTTGTTCATAGGCTACCAT+AGG | 0.522734 | 7.4:+44191229 | MS.gene78240:intron |
| AGGAACTACGACCAAACCTA+TGG | 0.545163 | 7.4:+44191312 | MS.gene78240:CDS |
| TGCAAAGTATGCAAGTCCTA+TGG | 0.552339 | 7.4:-44191245 | None:intergenic |
| AAGATCAAGAACCACCTAGC+GGG | 0.560815 | 7.4:+44191284 | MS.gene78240:CDS |
| ATCCTATTAGCCTCTTCCAA+AGG | 0.564567 | 7.4:-44191340 | None:intergenic |
| TATGGATGATCCTTTGGAAG+AGG | 0.564870 | 7.4:+44191330 | MS.gene78240:CDS |
| GTAGTTCCTCCATTCCCGCT+AGG | 0.572693 | 7.4:-44191298 | None:intergenic |
| TCTTCCAAAGGATCATCCAT+AGG | 0.581655 | 7.4:-44191328 | None:intergenic |
| AAATAAAGAAAGCTGTAGAG+AGG | 0.616626 | 7.4:-44190487 | None:intergenic |
| GTTCCTCCATTCCCGCTAGG+TGG | 0.621366 | 7.4:-44191295 | None:intergenic |
| GAACCACCTAGCGGGAATGG+AGG | 0.633606 | 7.4:+44191292 | MS.gene78240:CDS |
| CTGTACCAAGACCAATAGCA+AGG | 0.633937 | 7.4:+44190411 | MS.gene78240:CDS |
| AAAGATCAAGAACCACCTAG+CGG | 0.725332 | 7.4:+44191283 | MS.gene78240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATATGATATGAAAAA+TGG | - | chr7.4:44190524-44190543 | None:intergenic | 10.0% |
| !!! | TCATTTTTTTATTATCTTTG+TGG | + | chr7.4:44190012-44190031 | MS.gene78240:intron | 15.0% |
| !! | AATAAAACCTTAATCATACA+TGG | - | chr7.4:44189608-44189627 | None:intergenic | 20.0% |
| !! | AATAGTTGAGACTTATTTAT+AGG | - | chr7.4:44190683-44190702 | None:intergenic | 20.0% |
| !! | ATAGTTGAGACTTATTTATA+GGG | - | chr7.4:44190682-44190701 | None:intergenic | 20.0% |
| !! | ATATATACTGTTATGAATCA+TGG | - | chr7.4:44190829-44190848 | None:intergenic | 20.0% |
| !! | TTCCCTTTAATAACATAATA+TGG | - | chr7.4:44190890-44190909 | None:intergenic | 20.0% |
| !!! | AATTTTATGTCCTTCATAAT+TGG | + | chr7.4:44189924-44189943 | MS.gene78240:intron | 20.0% |
| !!! | ATTTTATGTCCTTCATAATT+GGG | + | chr7.4:44189925-44189944 | MS.gene78240:intron | 20.0% |
| !!! | TGTATGATTAAGGTTTTATT+TGG | + | chr7.4:44189608-44189627 | MS.gene78240:intron | 20.0% |
| ! | AAAAAATTCAACCCTACAAA+AGG | - | chr7.4:44190942-44190961 | None:intergenic | 25.0% |
| ! | AAAAATTCAACCCTACAAAA+GGG | - | chr7.4:44190941-44190960 | None:intergenic | 25.0% |
| ! | AAAATGAAAGAGCAATAGTT+AGG | - | chr7.4:44189999-44190018 | None:intergenic | 25.0% |
| ! | ATAGTGAATAAACCATAGTT+AGG | + | chr7.4:44190594-44190613 | MS.gene78240:intron | 25.0% |
| ! | GGCCATATTATGTTATTAAA+GGG | + | chr7.4:44190885-44190904 | MS.gene78240:intron | 25.0% |
| ! | GTAAAAAACTTGAAAACAAC+AGG | - | chr7.4:44190322-44190341 | None:intergenic | 25.0% |
| ! | GTTAGGTTATATGTAAACTA+TGG | + | chr7.4:44190611-44190630 | MS.gene78240:intron | 25.0% |
| ! | GTTTACATATAACCTAACTA+TGG | - | chr7.4:44190609-44190628 | None:intergenic | 25.0% |
| ! | TAGTTGAGACTTATTTATAG+GGG | - | chr7.4:44190681-44190700 | None:intergenic | 25.0% |
| ! | TGGCCATATTATGTTATTAA+AGG | + | chr7.4:44190884-44190903 | MS.gene78240:intron | 25.0% |
| ! | TTGTACTAAATCACTTATTG+CGG | - | chr7.4:44190198-44190217 | None:intergenic | 25.0% |
| !! | GCTAATTATGCTATATTTTG+TGG | + | chr7.4:44191190-44191209 | MS.gene78240:intron | 25.0% |
| !! | TTAAGTTGTTTATCCAAACA+CGG | + | chr7.4:44189859-44189878 | MS.gene78240:intron | 25.0% |
| !!! | CTTATTTTCATTAGTTCTCT+AGG | + | chr7.4:44189729-44189748 | MS.gene78240:intron | 25.0% |
| !!! | TACAAAGTATTGTCAAACAA+CGG | + | chr7.4:44191044-44191063 | MS.gene78240:intron | 25.0% |
| AAATAAAGAAAGCTGTAGAG+AGG | - | chr7.4:44190490-44190509 | None:intergenic | 30.0% | |
| AAATGGTAATTGTGACTTAC+AGG | - | chr7.4:44190108-44190127 | None:intergenic | 30.0% | |
| AATGGTAATTGTGACTTACA+GGG | - | chr7.4:44190107-44190126 | None:intergenic | 30.0% | |
| ATATTTGTGCTATATGGTAG+CGG | + | chr7.4:44190149-44190168 | MS.gene78240:intron | 30.0% | |
| ATCCTAAAGTCTTAACCATT+GGG | - | chr7.4:44191147-44191166 | None:intergenic | 30.0% | |
| CAAAAGACAAAGCAATTATC+AGG | - | chr7.4:44189497-44189516 | None:intergenic | 30.0% | |
| CAAGTACATTGTTTGTTCAT+AGG | + | chr7.4:44191219-44191238 | MS.gene78240:intron | 30.0% | |
| CTTGATCTTTATTGAATGCT+AGG | - | chr7.4:44191273-44191292 | None:intergenic | 30.0% | |
| GAAAACACCATGTATGATTA+AGG | + | chr7.4:44189598-44189617 | MS.gene78240:intron | 30.0% | |
| GAGAAACATGAAAAACTAGA+AGG | - | chr7.4:44190358-44190377 | None:intergenic | 30.0% | |
| TAAGTCACAATTACCATTTC+CGG | + | chr7.4:44190109-44190128 | MS.gene78240:intron | 30.0% | |
| TAGCAGATATTTGTGCTATA+TGG | + | chr7.4:44190143-44190162 | MS.gene78240:intron | 30.0% | |
| TATCCTAAAGTCTTAACCAT+TGG | - | chr7.4:44191148-44191167 | None:intergenic | 30.0% | |
| TTAAGTGTTCCCAATTATGA+AGG | - | chr7.4:44189937-44189956 | None:intergenic | 30.0% | |
| TTCATTGTTCAACAAGTCTT+AGG | - | chr7.4:44189885-44189904 | None:intergenic | 30.0% | |
| ! | AAGGATTTCAGTAACTTTTG+TGG | + | chr7.4:44190430-44190449 | MS.gene78240:CDS | 30.0% |
| ! | ATATGTCTTTTCCCTTTTGT+AGG | + | chr7.4:44190927-44190946 | MS.gene78240:intron | 30.0% |
| ! | CTATGGTTAGTTAGTTAGTT+AGG | + | chr7.4:44190628-44190647 | MS.gene78240:intron | 30.0% |
| ! | CTGATAATTGCTTTGTCTTT+TGG | + | chr7.4:44189495-44189514 | MS.gene78240:intron | 30.0% |
| ! | TATGTCTTTTCCCTTTTGTA+GGG | + | chr7.4:44190928-44190947 | MS.gene78240:intron | 30.0% |
| ! | TCTTAAAGTGCTTACATCAT+TGG | - | chr7.4:44189654-44189673 | None:intergenic | 30.0% |
| ! | TGGTGTTTTCCTTTCAAAAT+AGG | - | chr7.4:44189588-44189607 | None:intergenic | 30.0% |
| !! | CTCTACAGCTTTCTTTATTT+TGG | + | chr7.4:44190490-44190509 | MS.gene78240:CDS | 30.0% |
| !! | TGTCTTTTGGAAATTCATGT+GGG | + | chr7.4:44189508-44189527 | MS.gene78240:intron | 30.0% |
| !! | TTGTCTTTTGGAAATTCATG+TGG | + | chr7.4:44189507-44189526 | MS.gene78240:intron | 30.0% |
| AAGTCACAATTACCATTTCC+GGG | + | chr7.4:44190110-44190129 | MS.gene78240:intron | 35.0% | |
| AATCTACTATGAGTCACTGT+GGG | + | chr7.4:44190736-44190755 | MS.gene78240:intron | 35.0% | |
| ACATGAATGAACATGGCAAT+TGG | - | chr7.4:44190862-44190881 | None:intergenic | 35.0% | |
| ATAGCATAGGTGTATTCATG+TGG | - | chr7.4:44190768-44190787 | None:intergenic | 35.0% | |
| ATCAGCAAGAAAGATAGCAT+AGG | - | chr7.4:44190781-44190800 | None:intergenic | 35.0% | |
| ATCATTATGACCTTTATCCC+TGG | + | chr7.4:44190799-44190818 | MS.gene78240:intron | 35.0% | |
| ATTGACAACAAGTTTCCCAA+TGG | + | chr7.4:44191129-44191148 | MS.gene78240:intron | 35.0% | |
| GAGTTTATGCATTTGTGATG+AGG | + | chr7.4:44189559-44189578 | MS.gene78240:intron | 35.0% | |
| TAATCTACTATGAGTCACTG+TGG | + | chr7.4:44190735-44190754 | MS.gene78240:intron | 35.0% | |
| TAGCATAGGTGTATTCATGT+GGG | - | chr7.4:44190767-44190786 | None:intergenic | 35.0% | |
| TCATTATGACCTTTATCCCT+GGG | + | chr7.4:44190800-44190819 | MS.gene78240:intron | 35.0% | |
| TCTAGCATCAGCAAAAAGAA+GGG | - | chr7.4:44190388-44190407 | None:intergenic | 35.0% | |
| TTATGAATCATGGAATCCCA+GGG | - | chr7.4:44190819-44190838 | None:intergenic | 35.0% | |
| TTCCCAATGGTTAAGACTTT+AGG | + | chr7.4:44191142-44191161 | MS.gene78240:intron | 35.0% | |
| TTCTAGCATCAGCAAAAAGA+AGG | - | chr7.4:44190389-44190408 | None:intergenic | 35.0% | |
| ! | GTTACTGAAATCCTTGCTAT+TGG | - | chr7.4:44190425-44190444 | None:intergenic | 35.0% |
| ! | TACAAAGTCTTGTCGAATAG+TGG | + | chr7.4:44190213-44190232 | MS.gene78240:intron | 35.0% |
| !! | ATGAGGCATCCTATTTTGAA+AGG | + | chr7.4:44189576-44189595 | MS.gene78240:intron | 35.0% |
| !! | GGGAAACTTGTTGTCAATTA+CGG | - | chr7.4:44191127-44191146 | None:intergenic | 35.0% |
| !! | GTAAGCACTTTAAGACTGTT+TGG | + | chr7.4:44189659-44189678 | MS.gene78240:intron | 35.0% |
| AAAGATCAAGAACCACCTAG+CGG | + | chr7.4:44191283-44191302 | MS.gene78240:CDS | 40.0% | |
| AGAGCAATAGTTAGGCTTTC+TGG | - | chr7.4:44189991-44190010 | None:intergenic | 40.0% | |
| ATCACTTATTGCGGAACAAC+AGG | - | chr7.4:44190189-44190208 | None:intergenic | 40.0% | |
| ATTATAGCACTGCAGTGTAG+CGG | + | chr7.4:44190249-44190268 | MS.gene78240:intron | 40.0% | |
| CAAACCTATGGATGATCCTT+TGG | + | chr7.4:44191324-44191343 | MS.gene78240:CDS | 40.0% | |
| CAAAGGATCATCCATAGGTT+TGG | - | chr7.4:44191326-44191345 | None:intergenic | 40.0% | |
| GGAAGAGGCTAATAGGATAA+TGG | + | chr7.4:44191345-44191364 | MS.gene78240:CDS | 40.0% | |
| GTTATGAATCATGGAATCCC+AGG | - | chr7.4:44190820-44190839 | None:intergenic | 40.0% | |
| GTTTGTTCATAGGCTACCAT+AGG | + | chr7.4:44191229-44191248 | MS.gene78240:intron | 40.0% | |
| TATTTGACAATGCCCGGAAA+TGG | - | chr7.4:44190125-44190144 | None:intergenic | 40.0% | |
| TCGAATAGTGGCTATAACAG+TGG | + | chr7.4:44190225-44190244 | MS.gene78240:intron | 40.0% | |
| TCTTCCAAAGGATCATCCAT+AGG | - | chr7.4:44191331-44191350 | None:intergenic | 40.0% | |
| TGCAAAGTATGCAAGTCCTA+TGG | - | chr7.4:44191248-44191267 | None:intergenic | 40.0% | |
| TGCCATGTTCATTCATGTAC+TGG | + | chr7.4:44190864-44190883 | MS.gene78240:intron | 40.0% | |
| ! | AGATAGTTCTGCAGAATGCA+TGG | + | chr7.4:44190040-44190059 | MS.gene78240:intron | 40.0% |
| ! | ATAGCTGCTGTTTGAAACAG+TGG | - | chr7.4:44190975-44190994 | None:intergenic | 40.0% |
| ! | ATCCTATTAGCCTCTTCCAA+AGG | - | chr7.4:44191343-44191362 | None:intergenic | 40.0% |
| ! | ATCCTTTGGAAGAGGCTAAT+AGG | + | chr7.4:44191338-44191357 | MS.gene78240:CDS | 40.0% |
| ! | ATCTGCTATTTGACAATGCC+CGG | - | chr7.4:44190131-44190150 | None:intergenic | 40.0% |
| ! | TATGGATGATCCTTTGGAAG+AGG | + | chr7.4:44191330-44191349 | MS.gene78240:CDS | 40.0% |
| !! | GAAATCCTTGCTATTGGTCT+TGG | - | chr7.4:44190419-44190438 | None:intergenic | 40.0% |
| !!! | ACAGGCAGTTTTTTCAATCG+CGG | - | chr7.4:44190304-44190323 | None:intergenic | 40.0% |
| AACAAGTCTTAGGCCGTGTT+TGG | - | chr7.4:44189875-44189894 | None:intergenic | 45.0% | |
| AAGATCAAGAACCACCTAGC+GGG | + | chr7.4:44191284-44191303 | MS.gene78240:CDS | 45.0% | |
| AGGAACTACGACCAAACCTA+TGG | + | chr7.4:44191312-44191331 | MS.gene78240:CDS | 45.0% | |
| CAATCGCGGATCACAGAAAA+TGG | - | chr7.4:44190290-44190309 | None:intergenic | 45.0% | |
| CTGTACCAAGACCAATAGCA+AGG | + | chr7.4:44190411-44190430 | MS.gene78240:CDS | 45.0% | |
| GGCCAGTACATGAATGAACA+TGG | - | chr7.4:44190869-44190888 | None:intergenic | 45.0% | |
| GGTAAGGATGGAATTGATGC+AGG | + | chr7.4:44190069-44190088 | MS.gene78240:intron | 45.0% | |
| TCATGGAATCCCAGGGATAA+AGG | - | chr7.4:44190812-44190831 | None:intergenic | 45.0% | |
| !! | GAATGCATGGAGAGTTGGTA+AGG | + | chr7.4:44190053-44190072 | MS.gene78240:intron | 45.0% |
| CTGCAGAATGCATGGAGAGT+TGG | + | chr7.4:44190048-44190067 | MS.gene78240:intron | 50.0% | |
| !! | GCATGGAGAGTTGGTAAGGA+TGG | + | chr7.4:44190057-44190076 | MS.gene78240:intron | 50.0% |
| CAAGAACCACCTAGCGGGAA+TGG | + | chr7.4:44191289-44191308 | MS.gene78240:CDS | 55.0% | |
| GTAGTTCCTCCATTCCCGCT+AGG | - | chr7.4:44191301-44191320 | None:intergenic | 55.0% | |
| GAACCACCTAGCGGGAATGG+AGG | + | chr7.4:44191292-44191311 | MS.gene78240:CDS | 60.0% | |
| GTTCCTCCATTCCCGCTAGG+TGG | - | chr7.4:44191298-44191317 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 44189469 | 44191381 | 44189469 | ID=MS.gene78240 |
| chr7.4 | mRNA | 44189469 | 44191381 | 44189469 | ID=MS.gene78240.t1;Parent=MS.gene78240 |
| chr7.4 | exon | 44189469 | 44189480 | 44189469 | ID=MS.gene78240.t1.exon1;Parent=MS.gene78240.t1 |
| chr7.4 | CDS | 44189469 | 44189480 | 44189469 | ID=cds.MS.gene78240.t1;Parent=MS.gene78240.t1 |
| chr7.4 | exon | 44190407 | 44190511 | 44190407 | ID=MS.gene78240.t1.exon2;Parent=MS.gene78240.t1 |
| chr7.4 | CDS | 44190407 | 44190511 | 44190407 | ID=cds.MS.gene78240.t1;Parent=MS.gene78240.t1 |
| chr7.4 | exon | 44191241 | 44191381 | 44191241 | ID=MS.gene78240.t1.exon3;Parent=MS.gene78240.t1 |
| chr7.4 | CDS | 44191241 | 44191381 | 44191241 | ID=cds.MS.gene78240.t1;Parent=MS.gene78240.t1 |
| Gene Sequence |
| Protein sequence |
