Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79632.t1 | XP_003621137.1 | 94.6 | 260 | 13 | 1 | 1 | 260 | 1 | 259 | 1.30E-125 | 459.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79632.t1 | P0A957 | 28.8 | 191 | 136 | 0 | 60 | 250 | 17 | 207 | 3.0e-17 | 90.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79632.t1 | A2Q5T0 | 94.6 | 260 | 13 | 1 | 1 | 260 | 1 | 259 | 9.5e-126 | 459.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene79632 | 0.805825 | 1.11E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene79632 | 0.820812 | 5.63E-53 | -1.69E-46 |
| MS.gene050099 | MS.gene79632 | 0.804724 | 1.88E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene79632 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene79632 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene79632 | 0.847111 | 1.42E-59 | -1.69E-46 |
| MS.gene051663 | MS.gene79632 | 0.822003 | 2.99E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene79632 | 0.833568 | 4.95E-56 | -1.69E-46 |
| MS.gene053907 | MS.gene79632 | 0.804962 | 1.68E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene79632 | 0.811797 | 5.85E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene79632 | 0.814136 | 1.80E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene79632 | 0.818556 | 1.84E-52 | -1.69E-46 |
| MS.gene055428 | MS.gene79632 | 0.810976 | 8.82E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene79632 | 0.816063 | 6.71E-52 | -1.69E-46 |
| MS.gene057121 | MS.gene79632 | 0.816716 | 4.79E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene79632 | 0.847791 | 9.25E-60 | -1.69E-46 |
| MS.gene058002 | MS.gene79632 | 0.806844 | 6.75E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene79632 | 0.809728 | 1.64E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene79632 | 0.809638 | 1.71E-50 | -1.69E-46 |
| MS.gene059894 | MS.gene79632 | 0.802849 | 4.62E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene79632 | 0.804237 | 2.38E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene79632 | 0.813204 | 2.88E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene79632 | 0.817218 | 3.70E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79632.t1 | MTR_7g009670 | 94.615 | 260 | 13 | 1 | 1 | 260 | 1 | 259 | 2.34e-170 | 471 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGTTTATCAGGATATTT+TGG | 0.153821 | 7.3:-88443443 | MS.gene79632:intron |
| TTCAATGGAGGAATATATTA+TGG | 0.223242 | 7.3:-88440691 | MS.gene79632:CDS |
| TGTCTTCTTCAACTTCTTCT+AGG | 0.228202 | 7.3:-88447131 | MS.gene79632:CDS |
| TGTTTCTATGGTTGCTTCTC+AGG | 0.260595 | 7.3:-88441309 | MS.gene79632:CDS |
| GCCCTTTCCTCATGTTTCTA+TGG | 0.281002 | 7.3:-88441321 | MS.gene79632:CDS |
| TTTCAAACCTTAACATTTGC+AGG | 0.284968 | 7.3:+88443639 | None:intergenic |
| CATACCCCTAGAGTTATTCT+TGG | 0.300250 | 7.3:+88444751 | None:intergenic |
| TTCGGTTGGAGTCATTGTTC+CGG | 0.335610 | 7.3:+88443387 | None:intergenic |
| CATGTAACCCTGCTAGGATT+AGG | 0.336708 | 7.3:+88447102 | None:intergenic |
| CTTAGGTTGGAACTGTTCTC+AGG | 0.354628 | 7.3:-88443713 | MS.gene79632:intron |
| CTCTGCCAAATCAAGAATTC+TGG | 0.354839 | 7.3:-88447024 | MS.gene79632:CDS |
| CAAATTGTTTGTAGATTCAA+TGG | 0.355850 | 7.3:-88440706 | MS.gene79632:intron |
| TCGGTTGGAGTCATTGTTCC+GGG | 0.368934 | 7.3:+88443388 | None:intergenic |
| TGCACAAACTGCTACCTTGT+TGG | 0.380160 | 7.3:-88440583 | MS.gene79632:CDS |
| AATGAGTCCTGCAAATGTTA+AGG | 0.388475 | 7.3:-88443646 | MS.gene79632:intron |
| AATCCTCCAAGTGCTGAAAC+TGG | 0.391654 | 7.3:+88441368 | None:intergenic |
| GCACAAACTGCTACCTTGTT+GGG | 0.392568 | 7.3:-88440582 | MS.gene79632:CDS |
| TGACACCAGAATTCTTGATT+TGG | 0.399723 | 7.3:+88447019 | None:intergenic |
| TTGACATTGGTACATGGTTA+AGG | 0.413248 | 7.3:+88447158 | None:intergenic |
| GTCTACCCCTGGTGTGTTTG+AGG | 0.433621 | 7.3:-88444882 | MS.gene79632:intron |
| TGAACTACACTTACTTCGGT+TGG | 0.441137 | 7.3:+88443373 | None:intergenic |
| ATATGATGCAGGTGCTAAAA+TGG | 0.441229 | 7.3:-88443039 | MS.gene79632:CDS |
| ATTTATCCAGTTTCAGCACT+TGG | 0.444137 | 7.3:-88441374 | MS.gene79632:CDS |
| GCTTCTCAGGGAATAACTAT+AGG | 0.445033 | 7.3:-88441296 | MS.gene79632:intron |
| GGCGTCTTCCTAATCCTAGC+AGG | 0.462294 | 7.3:-88447110 | MS.gene79632:CDS |
| AGATGCTATATTTGATAAAG+AGG | 0.471242 | 7.3:-88440643 | MS.gene79632:CDS |
| GTTTCTATGGTTGCTTCTCA+GGG | 0.474075 | 7.3:-88441308 | MS.gene79632:CDS |
| GCTGCAAATGCTGCAATAGC+AGG | 0.475105 | 7.3:-88445398 | MS.gene79632:CDS |
| AATGTCACTTATTCACAGCT+TGG | 0.480387 | 7.3:+88440556 | None:intergenic |
| TCAATGGAGGAATATATTAT+GGG | 0.482009 | 7.3:-88440690 | MS.gene79632:CDS |
| TTGCTTGTCTCAGAGCCAAT+AGG | 0.494076 | 7.3:-88446996 | MS.gene79632:intron |
| ACCATAGAAACATGAGGAAA+GGG | 0.499530 | 7.3:+88441320 | None:intergenic |
| TGGAACTGTTCTCAGGATTG+AGG | 0.505369 | 7.3:-88443706 | MS.gene79632:CDS |
| CAACCATTGACATTGGTACA+TGG | 0.515672 | 7.3:+88447152 | None:intergenic |
| CACAGCTTGGTTACCCAACA+AGG | 0.521254 | 7.3:+88440569 | None:intergenic |
| ATATAGTGCAGAAGTGGCAT+TGG | 0.521761 | 7.3:-88445423 | MS.gene79632:intron |
| AGCATCCAAGAATAACTCTA+GGG | 0.524792 | 7.3:-88444756 | MS.gene79632:CDS |
| TAACCATGTACCAATGTCAA+TGG | 0.531872 | 7.3:-88447155 | MS.gene79632:CDS |
| GAGCATCCAAGAATAACTCT+AGG | 0.537480 | 7.3:-88444757 | MS.gene79632:CDS |
| ACTTTGCATGTAACCCTGCT+AGG | 0.542921 | 7.3:+88447096 | None:intergenic |
| AACCATAGAAACATGAGGAA+AGG | 0.555765 | 7.3:+88441319 | None:intergenic |
| ACTTACATATAGTGCAGAAG+TGG | 0.568106 | 7.3:-88445429 | MS.gene79632:intron |
| TGTGTAGGTTCTACAACAGC+TGG | 0.571829 | 7.3:-88444785 | MS.gene79632:intron |
| GAGATTGTGATGTCTACCCC+TGG | 0.574552 | 7.3:-88444893 | MS.gene79632:CDS |
| TATCCAGTTTCAGCACTTGG+AGG | 0.586579 | 7.3:-88441371 | MS.gene79632:CDS |
| TTACCAACCTCAAACACACC+AGG | 0.591489 | 7.3:+88444875 | None:intergenic |
| AAGAAGACAACCATTGACAT+TGG | 0.591717 | 7.3:+88447145 | None:intergenic |
| ATTGTTTGTAGATTCAATGG+AGG | 0.594558 | 7.3:-88440703 | MS.gene79632:intron |
| GGTTCTACAACAGCTGGTGA+AGG | 0.600587 | 7.3:-88444779 | MS.gene79632:CDS |
| GCAAATGCTGCAATAGCAGG+TGG | 0.601354 | 7.3:-88445395 | MS.gene79632:CDS |
| ATATTGTCTGCATATGATGC+AGG | 0.602331 | 7.3:-88443050 | MS.gene79632:CDS |
| TGCAGGTGCTAAAATGGTCA+AGG | 0.620940 | 7.3:-88443033 | MS.gene79632:intron |
| ACTCATTAGAAATTTAGCCC+CGG | 0.633975 | 7.3:+88443662 | None:intergenic |
| GCGTCTTCCTAATCCTAGCA+GGG | 0.636606 | 7.3:-88447109 | MS.gene79632:CDS |
| GGTTAAGGTTGTCCTTGCAG+AGG | 0.641587 | 7.3:+88447173 | None:intergenic |
| ACAAAACAATGACCTCTGCA+AGG | 0.651574 | 7.3:-88447185 | None:intergenic |
| ATGGAGGAATATATTATGGG+AGG | 0.662673 | 7.3:-88440687 | MS.gene79632:CDS |
| TACCAACCTCAAACACACCA+GGG | 0.665542 | 7.3:+88444876 | None:intergenic |
| GAAGCAACCATAGAAACATG+AGG | 0.669495 | 7.3:+88441314 | None:intergenic |
| GCATCCAAGAATAACTCTAG+GGG | 0.688062 | 7.3:-88444755 | MS.gene79632:intron |
| ACCAACCTCAAACACACCAG+GGG | 0.719426 | 7.3:+88444877 | None:intergenic |
| GTGGTGGATACTGAAAGCTG+AGG | 0.757250 | 7.3:+88447060 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 88440560 | 88447199 | 88440560 | ID=MS.gene79632 |
| chr7.3 | mRNA | 88440560 | 88447199 | 88440560 | ID=MS.gene79632.t1;Parent=MS.gene79632 |
| chr7.3 | exon | 88446997 | 88447199 | 88446997 | ID=MS.gene79632.t1.exon1;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88446997 | 88447199 | 88446997 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88445385 | 88445439 | 88445385 | ID=MS.gene79632.t1.exon2;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88445385 | 88445439 | 88445385 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88444883 | 88444918 | 88444883 | ID=MS.gene79632.t1.exon3;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88444883 | 88444918 | 88444883 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88444756 | 88444800 | 88444756 | ID=MS.gene79632.t1.exon4;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88444756 | 88444800 | 88444756 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88443647 | 88443730 | 88443647 | ID=MS.gene79632.t1.exon5;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88443647 | 88443730 | 88443647 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88443387 | 88443452 | 88443387 | ID=MS.gene79632.t1.exon6;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88443387 | 88443452 | 88443387 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88443034 | 88443072 | 88443034 | ID=MS.gene79632.t1.exon7;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88443034 | 88443072 | 88443034 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88441297 | 88441396 | 88441297 | ID=MS.gene79632.t1.exon8;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88441297 | 88441396 | 88441297 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| chr7.3 | exon | 88440560 | 88440714 | 88440560 | ID=MS.gene79632.t1.exon9;Parent=MS.gene79632.t1 |
| chr7.3 | CDS | 88440560 | 88440714 | 88440560 | ID=cds.MS.gene79632.t1;Parent=MS.gene79632.t1 |
| Gene Sequence |
| Protein sequence |
