AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80664


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80664.t1 MTR_5g082070 97.351 151 2 1 1 149 139 289 3.09e-100 289
MS.gene80664.t1 MTR_3g070210 90.728 151 12 1 1 149 139 289 3.00e-92 269
MS.gene80664.t1 MTR_8g098375 85.430 151 20 1 1 149 136 286 3.14e-89 261
MS.gene80664.t1 MTR_5g010150 84.768 151 21 1 1 149 136 286 5.56e-88 258
MS.gene80664.t1 MTR_4g415300 79.470 151 28 2 1 149 140 289 7.46e-80 238
MS.gene80664.t1 MTR_3g070210 88.800 125 12 1 1 123 139 263 2.32e-72 218
MS.gene80664.t1 MTR_7g101190 71.724 145 38 2 1 143 129 272 1.34e-69 211
MS.gene80664.t1 MTR_1g095070 72.414 145 37 2 1 143 63 206 4.85e-69 208
MS.gene80664.t1 MTR_3g118010 70.667 150 41 2 1 148 121 269 3.55e-68 207
MS.gene80664.t1 MTR_2g094270 66.667 150 47 2 1 148 131 279 3.28e-67 205
MS.gene80664.t1 MTR_4g059390 66.000 150 48 2 1 148 129 277 1.08e-63 196
MS.gene80664.t1 MTR_1g095070 64.539 141 35 3 1 139 63 190 5.68e-56 174
MS.gene80664.t1 MTR_2g094270 69.421 121 34 2 1 119 131 250 2.72e-54 172
MS.gene80664.t1 MTR_8g098375 77.647 85 19 0 1 85 136 220 2.73e-40 135
MS.gene80664.t1 MTR_3g070210 87.671 73 9 0 1 73 139 211 9.68e-39 130
MS.gene80664.t1 MTR_5g010150 82.192 73 13 0 1 73 136 208 3.74e-37 127
MS.gene80664.t1 MTR_4g415300 62.069 87 32 1 1 87 140 225 3.24e-29 106
MS.gene80664.t1 MTR_4g059390 65.753 73 24 1 1 73 129 200 7.18e-25 95.1
MS.gene80664.t1 MTR_6g069040 40.411 146 75 5 2 145 108 243 4.18e-16 72.8
MS.gene80664.t1 MTR_1g077540 36.986 146 80 5 2 145 108 243 1.04e-13 66.6
MS.gene80664.t1 MTR_2g101370 37.143 140 77 4 2 140 107 236 1.93e-13 65.9
MS.gene80664.t1 MTR_5g012810 42.857 105 54 3 34 137 132 231 8.27e-13 63.9
MS.gene80664.t1 MTR_4g063090 35.036 137 78 4 2 137 108 234 2.30e-12 62.8
MS.gene80664.t1 MTR_8g013680 41.667 96 50 3 43 137 142 232 6.74e-12 61.6
MS.gene80664.t1 MTR_1g115405 40.000 105 57 3 34 137 135 234 4.92e-11 59.3
MS.gene80664.t1 MTR_1g006490 38.182 110 62 3 25 133 120 224 7.29e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80664.t1 AT4G00430 84.768 151 20 2 1 149 137 286 3.44e-84 249
MS.gene80664.t1 AT3G61430 84.106 151 21 2 1 149 136 285 6.01e-84 248
MS.gene80664.t1 AT3G61430 84.106 151 21 2 1 149 136 285 6.01e-84 248
MS.gene80664.t1 AT2G45960 83.444 151 22 2 1 149 136 285 1.54e-83 247
MS.gene80664.t1 AT1G01620 83.444 151 22 2 1 149 64 213 5.08e-83 243
MS.gene80664.t1 AT1G01620 83.444 151 22 2 1 149 136 285 1.96e-82 244
MS.gene80664.t1 AT4G23400 84.106 151 21 2 1 149 137 286 4.79e-82 243
MS.gene80664.t1 AT2G37180 70.667 150 41 2 1 148 127 275 2.01e-68 208
MS.gene80664.t1 AT3G53420 70.395 152 38 3 1 148 129 277 1.52e-67 206
MS.gene80664.t1 AT3G53420 70.395 152 38 3 1 148 129 277 1.52e-67 206
MS.gene80664.t1 AT4G35100 70.000 150 42 2 1 148 122 270 1.53e-67 206
MS.gene80664.t1 AT4G35100 70.000 150 42 2 1 148 122 270 1.53e-67 206
MS.gene80664.t1 AT2G37170 70.000 150 42 2 1 148 170 318 3.49e-67 207
MS.gene80664.t1 AT2G16850 69.333 150 43 2 1 148 120 268 4.38e-67 205
MS.gene80664.t1 AT2G37170 70.000 150 42 2 1 148 127 275 4.53e-67 205
MS.gene80664.t1 AT3G54820 71.429 147 35 3 1 143 128 271 2.82e-66 203
MS.gene80664.t1 AT5G60660 68.421 152 41 3 1 148 129 277 3.68e-66 203
MS.gene80664.t1 AT2G39010 66.667 150 47 2 1 148 128 276 4.01e-65 200
MS.gene80664.t1 AT2G45960 82.645 121 18 2 1 119 136 255 2.75e-63 195
MS.gene80664.t1 AT2G45960 82.645 121 18 2 1 119 136 255 1.85e-62 194
MS.gene80664.t1 AT4G00430 74.684 79 19 1 1 79 137 214 8.48e-33 115
MS.gene80664.t1 AT2G39010 64.384 73 25 1 1 73 128 199 1.54e-24 94.7
MS.gene80664.t1 AT1G17810 45.133 113 55 4 34 144 143 250 1.75e-16 74.3
MS.gene80664.t1 AT1G73190 39.726 146 76 5 2 145 116 251 2.07e-16 73.9
MS.gene80664.t1 AT4G01470 37.226 137 75 4 2 137 108 234 5.31e-14 67.4
MS.gene80664.t1 AT5G47450 38.686 137 73 6 2 137 106 232 3.61e-13 65.1
MS.gene80664.t1 AT4G17340 41.905 105 55 3 34 137 133 232 6.07e-13 64.3
MS.gene80664.t1 AT3G16240 40.952 105 56 3 34 137 133 232 1.89e-12 63.2
MS.gene80664.t1 AT2G25810 38.182 110 61 4 34 141 129 233 3.66e-12 62.4

Find 47 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATACCAGTTCCAGTAAT+TGG 0.193062 5.4:+64721934 None:intergenic
TTCAGGGTGGGACCATTTAT+TGG 0.238516 5.4:-64721725 MS.gene80664:CDS
TATTCTTGTAGTGGATCTTC+AGG 0.243468 5.4:-64721742 MS.gene80664:intron
TTTGAAGGAAAGACAAGATT+TGG 0.252728 5.4:-64722355 MS.gene80664:CDS
CGCTGTGTTCTTGGTTCACT+TGG 0.261822 5.4:-64721965 MS.gene80664:CDS
GCAAGAGCTGCTCCAATAAA+TGG 0.306781 5.4:+64721713 None:intergenic
AATTGGGTTCGCTGTGTTCT+TGG 0.323972 5.4:-64721974 MS.gene80664:CDS
ACAAGATTTGGAGATCTAAA+AGG 0.340417 5.4:-64722343 MS.gene80664:CDS
CTACATGGTGATGCAATGTT+TGG 0.359716 5.4:-64722410 None:intergenic
TTGGGTGCTATTTGTGGTGC+TGG 0.389900 5.4:-64722391 MS.gene80664:CDS
TTGGCCACTATCCCAATTAC+TGG 0.392883 5.4:-64721946 MS.gene80664:CDS
ATTTGATCACTTTGACTTGA+AGG 0.406054 5.4:+64721655 None:intergenic
CAATGTTTGGGTGCTATTTG+TGG 0.409953 5.4:-64722397 MS.gene80664:CDS
TAATACCAGTTCCAGTAATT+GGG 0.410603 5.4:+64721935 None:intergenic
ACTATCCCAATTACTGGAAC+TGG 0.426227 5.4:-64721940 MS.gene80664:CDS
GGTGCCAACTTTGTTGCTCC+TGG 0.427799 5.4:-64722319 MS.gene80664:CDS
ATTCTTGTAGTGGATCTTCA+GGG 0.431921 5.4:-64721741 MS.gene80664:intron
AGCACCAAGACCATCACCTT+TGG 0.435976 5.4:+64722291 None:intergenic
TACATGGTGATGCAATGTTT+GGG 0.464448 5.4:-64722409 MS.gene80664:CDS
ATTAACCCTGCTCGTAGTCT+TGG 0.478309 5.4:-64721916 MS.gene80664:CDS
TGTGGTGCTGGTGTTGTGAA+AGG 0.479519 5.4:-64722379 MS.gene80664:CDS
TCTTCAACAAGGACCTTGGT+TGG 0.486748 5.4:-64721882 MS.gene80664:CDS
GGTCTTGGTGCTGAAATTGT+TGG 0.490732 5.4:-64722280 MS.gene80664:CDS
TACGGGAACATGAGAGTCTC+TGG 0.492319 5.4:+64722204 None:intergenic
AAGAACACAGCGAACCCAAT+TGG 0.494251 5.4:+64721976 None:intergenic
TCACTTTGACTTGAAGGGAA+TGG 0.512515 5.4:+64721661 None:intergenic
AGTTCCAGTAATTGGGATAG+TGG 0.513529 5.4:+64721942 None:intergenic
TTTGATCACTTTGACTTGAA+GGG 0.520518 5.4:+64721656 None:intergenic
ATTATCTTCAACAAGGACCT+TGG 0.525491 5.4:-64721886 MS.gene80664:CDS
GTTGCTCCTGGTTACACCAA+AGG 0.542015 5.4:-64722307 MS.gene80664:CDS
TACACCAAAGGTGATGGTCT+TGG 0.554514 5.4:-64722295 MS.gene80664:CDS
CCTGGTTACACCAAAGGTGA+TGG 0.563447 5.4:-64722301 MS.gene80664:CDS
CCATCACCTTTGGTGTAACC+AGG 0.566415 5.4:+64722301 None:intergenic
AGCATCGGTGGCTGAGAAGA+CGG 0.570780 5.4:+64722237 None:intergenic
GGCGCTACGTTTAGCATCGG+TGG 0.574723 5.4:+64722225 None:intergenic
TTGTAGTGGATCTTCAGGGT+GGG 0.576594 5.4:-64721737 MS.gene80664:intron
GCAGCACCAAGACTACGAGC+AGG 0.585086 5.4:+64721910 None:intergenic
CTTGTAGTGGATCTTCAGGG+TGG 0.618253 5.4:-64721738 MS.gene80664:intron
GTAACCAGGAGCAACAAAGT+TGG 0.636963 5.4:+64722315 None:intergenic
ACAGCGAACCCAATTGGCAA+TGG 0.642498 5.4:+64721982 None:intergenic
TGCTGCTATTATCTTCAACA+AGG 0.649253 5.4:-64721893 MS.gene80664:CDS
TCTGGCGCTACGTTTAGCAT+CGG 0.662595 5.4:+64722222 None:intergenic
CAGCACCAAGACTACGAGCA+GGG 0.662928 5.4:+64721911 None:intergenic
AGATTTGGAGATCTAAAAGG+TGG 0.683343 5.4:-64722340 MS.gene80664:CDS
TTACATGATCATTCCAACCA+AGG 0.692776 5.4:+64721869 None:intergenic
TGGCTCTGATTACGACCACG+TGG 0.744746 5.4:+64721681 None:intergenic
TCTTGCAGCACTATACCACG+TGG 0.793900 5.4:-64721696 MS.gene80664:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAACTAATTATTTGTG+TGG - chr5.4:64722037-64722056 MS.gene80664:intron 15.0%
!! ATTAATTATTATTCTTGTAG+TGG - chr5.4:64722316-64722335 MS.gene80664:CDS 15.0%
!! CATTATTAAGTGTATATTGT+CGG + chr5.4:64722000-64722019 None:intergenic 20.0%
!!! CTTATTTTATGAGTAGATTT+TGG - chr5.4:64722060-64722079 MS.gene80664:intron 20.0%
! TCAGATACTGGATATATAAT+TGG - chr5.4:64721964-64721983 MS.gene80664:CDS 25.0%
!!! CTATTTTTATAGTCAGATAC+TGG - chr5.4:64721952-64721971 MS.gene80664:CDS 25.0%
ACAAGATTTGGAGATCTAAA+AGG - chr5.4:64721724-64721743 MS.gene80664:CDS 30.0%
TAATACCAGTTCCAGTAATT+GGG + chr5.4:64722135-64722154 None:intergenic 30.0%
TGATGGTTATGATTGATTGA+CGG + chr5.4:64721901-64721920 None:intergenic 30.0%
TTAATACCAGTTCCAGTAAT+TGG + chr5.4:64722136-64722155 None:intergenic 30.0%
TTTGAAGGAAAGACAAGATT+TGG - chr5.4:64721712-64721731 MS.gene80664:CDS 30.0%
AGATTTGGAGATCTAAAAGG+TGG - chr5.4:64721727-64721746 MS.gene80664:CDS 35.0%
ATTATCTTCAACAAGGACCT+TGG - chr5.4:64722181-64722200 MS.gene80664:intron 35.0%
TGCTGCTATTATCTTCAACA+AGG - chr5.4:64722174-64722193 MS.gene80664:intron 35.0%
TTACATGATCATTCCAACCA+AGG + chr5.4:64722201-64722220 None:intergenic 35.0%
!! ATTCTTGTAGTGGATCTTCA+GGG - chr5.4:64722326-64722345 MS.gene80664:CDS 35.0%
!! TATTCTTGTAGTGGATCTTC+AGG - chr5.4:64722325-64722344 MS.gene80664:CDS 35.0%
ACTATCCCAATTACTGGAAC+TGG - chr5.4:64722127-64722146 MS.gene80664:intron 40.0%
AGTTCCAGTAATTGGGATAG+TGG + chr5.4:64722128-64722147 None:intergenic 40.0%
TGACGGTGTAAAGAGACTTA+CGG + chr5.4:64721884-64721903 None:intergenic 40.0%
! TTTTGGCACCATTGCCAATT+GGG - chr5.4:64722077-64722096 MS.gene80664:intron 40.0%
!! ATTTTGGCACCATTGCCAAT+TGG - chr5.4:64722076-64722095 MS.gene80664:intron 40.0%
!! CAATGTTTGGGTGCTATTTG+TGG - chr5.4:64721670-64721689 MS.gene80664:CDS 40.0%
!! GGTGTTGTGAAAGGTTTTGA+AGG - chr5.4:64721697-64721716 MS.gene80664:CDS 40.0%
!! TCACTTTGACTTGAAGGGAA+TGG + chr5.4:64722409-64722428 None:intergenic 40.0%
AAGAACACAGCGAACCCAAT+TGG + chr5.4:64722094-64722113 None:intergenic 45.0%
AATTGGGTTCGCTGTGTTCT+TGG - chr5.4:64722093-64722112 MS.gene80664:intron 45.0%
ATTAACCCTGCTCGTAGTCT+TGG - chr5.4:64722151-64722170 MS.gene80664:intron 45.0%
GACGGTGTAAAGAGACTTAC+GGG + chr5.4:64721883-64721902 None:intergenic 45.0%
GCAAGAGCTGCTCCAATAAA+TGG + chr5.4:64722357-64722376 None:intergenic 45.0%
GTAACCAGGAGCAACAAAGT+TGG + chr5.4:64721755-64721774 None:intergenic 45.0%
TCTTCAACAAGGACCTTGGT+TGG - chr5.4:64722185-64722204 MS.gene80664:intron 45.0%
TTCAGGGTGGGACCATTTAT+TGG - chr5.4:64722342-64722361 MS.gene80664:CDS 45.0%
TTGGCCACTATCCCAATTAC+TGG - chr5.4:64722121-64722140 MS.gene80664:intron 45.0%
! TACACCAAAGGTGATGGTCT+TGG - chr5.4:64721772-64721791 MS.gene80664:intron 45.0%
!! GGTCTTGGTGCTGAAATTGT+TGG - chr5.4:64721787-64721806 MS.gene80664:intron 45.0%
!! TTGTAGTGGATCTTCAGGGT+GGG - chr5.4:64722330-64722349 MS.gene80664:CDS 45.0%
!! AAATATTTATAATAATTTGA+TGG + chr5.4:64721918-64721937 None:intergenic 5.0%
ACAGCGAACCCAATTGGCAA+TGG + chr5.4:64722088-64722107 None:intergenic 50.0%
AGCACCAAGACCATCACCTT+TGG + chr5.4:64721779-64721798 None:intergenic 50.0%
CCTGGTTACACCAAAGGTGA+TGG - chr5.4:64721766-64721785 MS.gene80664:intron 50.0%
CGCTGTGTTCTTGGTTCACT+TGG - chr5.4:64722102-64722121 MS.gene80664:intron 50.0%
GTTGCTCCTGGTTACACCAA+AGG - chr5.4:64721760-64721779 MS.gene80664:intron 50.0%
TACGGGAACATGAGAGTCTC+TGG + chr5.4:64721866-64721885 None:intergenic 50.0%
TCTGGCGCTACGTTTAGCAT+CGG + chr5.4:64721848-64721867 None:intergenic 50.0%
TCTTGCAGCACTATACCACG+TGG - chr5.4:64722371-64722390 MS.gene80664:CDS 50.0%
! CCATCACCTTTGGTGTAACC+AGG + chr5.4:64721769-64721788 None:intergenic 50.0%
!! CTTGTAGTGGATCTTCAGGG+TGG - chr5.4:64722329-64722348 MS.gene80664:CDS 50.0%
!! TGTGGTGCTGGTGTTGTGAA+AGG - chr5.4:64721688-64721707 MS.gene80664:CDS 50.0%
!! TTGGGTGCTATTTGTGGTGC+TGG - chr5.4:64721676-64721695 MS.gene80664:CDS 50.0%
CAGCACCAAGACTACGAGCA+GGG + chr5.4:64722159-64722178 None:intergenic 55.0%
GGTGCCAACTTTGTTGCTCC+TGG - chr5.4:64721748-64721767 MS.gene80664:intron 55.0%
!! AGCATCGGTGGCTGAGAAGA+CGG + chr5.4:64721833-64721852 None:intergenic 55.0%
!! TGGCTCTGATTACGACCACG+TGG + chr5.4:64722389-64722408 None:intergenic 55.0%
GCAGCACCAAGACTACGAGC+AGG + chr5.4:64722160-64722179 None:intergenic 60.0%
! GGCGCTACGTTTAGCATCGG+TGG + chr5.4:64721845-64721864 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 64721661 64722428 64721661 ID=MS.gene80664
chr5.4 mRNA 64721661 64722428 64721661 ID=MS.gene80664.t1;Parent=MS.gene80664
chr5.4 exon 64722213 64722428 64722213 ID=MS.gene80664.t1.exon1;Parent=MS.gene80664.t1
chr5.4 CDS 64722213 64722428 64722213 ID=cds.MS.gene80664.t1;Parent=MS.gene80664.t1
chr5.4 exon 64721873 64722013 64721873 ID=MS.gene80664.t1.exon2;Parent=MS.gene80664.t1
chr5.4 CDS 64721873 64722013 64721873 ID=cds.MS.gene80664.t1;Parent=MS.gene80664.t1
chr5.4 exon 64721661 64721753 64721661 ID=MS.gene80664.t1.exon3;Parent=MS.gene80664.t1
chr5.4 CDS 64721661 64721753 64721661 ID=cds.MS.gene80664.t1;Parent=MS.gene80664.t1
Gene Sequence

>MS.gene80664

ATGGTGATGCAATGTTTGGGTGCTATTTGTGGTGCTGGTGTTGTGAAAGGTTTTGAAGGAAAGACAAGATTTGGAGATCTAAAAGGTGGTGCCAACTTTGTTGCTCCTGGTTACACCAAAGGTGATGGTCTTGGTGCTGAAATTGTTGGCACTTTCATTCTTGTTTACACCGTCTTCTCAGCCACCGATGCTAAACGTAGCGCCAGAGACTCTCATGTTCCCGTAAGTCTCTTTACACCGTCAATCAATCATAACCATCAAATTATTATAAATATTTGACTTTAGTCGTAACTATTTTTATAGTCAGATACTGGATATATAATTGGTTGTGATTTACCGACAATATACACTTAATAATGTTAATTTTTACATGAATAATATAACTAATTATTTGTGTGGCTTATTTTATGAGTAGATTTTGGCACCATTGCCAATTGGGTTCGCTGTGTTCTTGGTTCACTTGGCCACTATCCCAATTACTGGAACTGGTATTAACCCTGCTCGTAGTCTTGGTGCTGCTATTATCTTCAACAAGGACCTTGGTTGGAATGATCATGTAAGTTGAAAAATTGAAATTAGTTATAAATTCATATTGTTTTTGTAAAATTATATATTTATTTTTCTGAATTTTCTATCTCATTTATCTTGTGTAGTCATTAATTATTATTCTTGTAGTGGATCTTCAGGGTGGGACCATTTATTGGAGCAGCTCTTGCAGCACTATACCACGTGGTCGTAATCAGAGCCATTCCCTTCAAGTCAAAGTGA

Protein sequence

>MS.gene80664.t1

MVMQCLGAICGAGVVKGFEGKTRFGDLKGGANFVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRSARDSHILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDLGWNDHWIFRVGPFIGAALAALYHVVVIRAIPFKSK