AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80793

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80793.t1 MTR_5g082070 99.654 289 1 0 1 289 1 289 0.0 584
MS.gene80793.t1 MTR_3g070210 92.734 289 21 0 1 289 1 289 0.0 548
MS.gene80793.t1 MTR_8g098375 86.713 286 36 1 4 289 3 286 0.0 511
MS.gene80793.t1 MTR_5g010150 84.965 286 41 1 4 289 3 286 4.87e-180 497
MS.gene80793.t1 MTR_3g070210 91.985 262 21 0 1 262 1 262 1.51e-178 493
MS.gene80793.t1 MTR_4g415300 80.690 290 54 2 1 289 1 289 3.80e-173 480
MS.gene80793.t1 MTR_3g070210 91.080 213 19 0 1 213 1 213 6.10e-143 400
MS.gene80793.t1 MTR_7g101190 72.868 258 63 2 32 283 16 272 2.70e-135 384
MS.gene80793.t1 MTR_2g094270 68.939 264 74 2 32 288 17 279 1.39e-133 380
MS.gene80793.t1 MTR_8g098375 80.349 229 41 2 4 230 3 229 2.86e-132 374
MS.gene80793.t1 MTR_3g118010 71.595 257 72 1 32 288 14 269 4.33e-132 376
MS.gene80793.t1 MTR_4g059390 68.015 272 80 3 23 288 7 277 2.83e-130 371
MS.gene80793.t1 MTR_5g010150 82.857 210 34 1 4 213 3 210 1.01e-125 357
MS.gene80793.t1 MTR_4g415300 77.103 214 47 2 1 213 1 213 2.80e-119 341
MS.gene80793.t1 MTR_2g094270 70.213 235 62 2 32 259 17 250 7.35e-119 342
MS.gene80793.t1 MTR_1g095070 77.297 185 41 1 99 283 23 206 5.95e-100 292
MS.gene80793.t1 MTR_4g059390 67.513 197 57 3 23 213 7 202 7.39e-88 261
MS.gene80793.t1 MTR_1g095070 70.718 181 40 2 99 279 23 190 3.16e-85 254
MS.gene80793.t1 MTR_6g069040 39.149 235 127 6 55 285 21 243 1.25e-32 120
MS.gene80793.t1 MTR_2g101370 36.364 231 132 5 50 277 15 233 1.15e-31 118
MS.gene80793.t1 MTR_1g077540 37.069 232 127 7 55 281 21 238 1.43e-31 118
MS.gene80793.t1 MTR_8g013680 36.910 233 130 6 50 277 12 232 7.95e-31 116
MS.gene80793.t1 MTR_5g012810 35.841 226 129 5 55 277 19 231 1.64e-27 107
MS.gene80793.t1 MTR_4g063090 33.333 237 143 5 44 277 10 234 8.82e-27 105
MS.gene80793.t1 MTR_1g115405 33.333 243 135 7 44 277 10 234 1.79e-26 104
MS.gene80793.t1 MTR_7g103030 33.878 245 141 8 45 283 11 240 2.57e-26 104
MS.gene80793.t1 MTR_1g006490 35.135 222 125 7 55 273 19 224 2.16e-25 101
MS.gene80793.t1 MTR_1g006590 35.345 232 119 10 55 277 19 228 6.56e-24 97.8
MS.gene80793.t1 MTR_7g023350 34.052 232 126 7 55 277 21 234 6.61e-24 97.8
MS.gene80793.t1 MTR_5g079570 27.481 262 159 6 25 275 2 243 1.58e-21 91.7
MS.gene80793.t1 MTR_2g017660 30.093 216 129 6 58 270 42 238 5.65e-20 87.8
MS.gene80793.t1 MTR_2g060360 30.901 233 131 7 55 277 19 231 6.81e-20 87.0
MS.gene80793.t1 MTR_4g084880 26.923 234 150 6 58 287 38 254 1.11e-19 86.7
MS.gene80793.t1 MTR_8g087710 30.126 239 144 8 53 287 37 256 1.49e-18 83.6
MS.gene80793.t1 MTR_5g063930 30.131 229 143 7 50 277 32 244 2.28e-18 82.8
MS.gene80793.t1 MTR_2g017590 32.407 216 124 6 58 270 39 235 3.30e-18 82.8
MS.gene80793.t1 MTR_7g010650 28.139 231 143 7 53 280 35 245 6.08e-17 79.3
MS.gene80793.t1 MTR_3g008920 27.354 223 143 4 58 277 25 231 9.16e-17 78.6
MS.gene80793.t1 MTR_2g017620 31.674 221 129 6 53 270 22 223 9.77e-17 78.2
MS.gene80793.t1 MTR_2g017620 31.674 221 129 6 53 270 36 237 1.18e-16 78.2
MS.gene80793.t1 MTR_4g075570 31.720 186 106 7 102 272 95 274 1.21e-16 79.0
MS.gene80793.t1 MTR_8g087720 28.448 232 143 8 52 279 35 247 1.83e-16 77.8
MS.gene80793.t1 MTR_2g017610 32.258 217 123 8 58 270 41 237 2.90e-16 77.4
MS.gene80793.t1 MTR_2g017570 31.019 216 127 7 58 270 41 237 4.93e-16 76.6
MS.gene80793.t1 MTR_4g075590 25.532 235 159 5 47 270 29 258 1.20e-15 76.3
MS.gene80793.t1 MTR_4g006730 26.923 234 143 7 37 270 68 273 2.42e-15 75.1
MS.gene80793.t1 MTR_2g104940 29.167 216 128 7 59 270 84 278 3.77e-14 71.6
MS.gene80793.t1 MTR_4g006630 29.050 179 113 4 92 270 22 186 1.22e-12 66.2
MS.gene80793.t1 MTR_1g097840 26.667 225 147 6 59 282 78 285 1.26e-12 67.4
MS.gene80793.t1 MTR_4g006730 29.050 179 113 4 92 270 22 186 1.34e-12 66.2
MS.gene80793.t1 MTR_2g017620 33.529 170 94 6 104 270 3 156 1.46e-12 65.5
MS.gene80793.t1 MTR_1g070385 26.667 225 146 6 52 275 59 265 2.86e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80793.t1 AT4G00430 85.813 289 38 2 1 289 1 286 0.0 501
MS.gene80793.t1 AT2G45960 83.737 289 43 2 1 289 1 285 3.00e-178 493
MS.gene80793.t1 AT4G23400 84.775 289 41 2 1 289 1 286 1.33e-168 468
MS.gene80793.t1 AT3G61430 84.083 289 42 2 1 289 1 285 1.58e-168 468
MS.gene80793.t1 AT3G61430 84.083 289 42 2 1 289 1 285 1.58e-168 468
MS.gene80793.t1 AT1G01620 84.083 289 42 2 1 289 1 285 1.63e-167 466
MS.gene80793.t1 AT2G45960 83.012 259 40 2 1 259 1 255 3.25e-156 437
MS.gene80793.t1 AT2G45960 83.012 259 40 2 1 259 1 255 7.56e-155 434
MS.gene80793.t1 AT2G37180 71.863 263 67 3 32 288 14 275 1.24e-133 380
MS.gene80793.t1 AT2G37170 71.863 263 67 3 32 288 57 318 7.85e-133 379
MS.gene80793.t1 AT2G37170 71.863 263 67 3 32 288 14 275 1.62e-132 377
MS.gene80793.t1 AT4G35100 71.984 257 71 1 32 288 15 270 1.73e-132 377
MS.gene80793.t1 AT4G35100 71.984 257 71 1 32 288 15 270 1.73e-132 377
MS.gene80793.t1 AT3G53420 72.453 265 62 4 32 288 16 277 1.99e-132 377
MS.gene80793.t1 AT3G53420 72.453 265 62 4 32 288 16 277 1.99e-132 377
MS.gene80793.t1 AT2G16850 71.595 257 72 1 32 288 13 268 1.20e-130 372
MS.gene80793.t1 AT1G01620 86.449 214 28 1 76 289 1 213 3.36e-130 369
MS.gene80793.t1 AT3G54820 69.434 265 70 3 32 288 15 276 1.27e-129 370
MS.gene80793.t1 AT4G00430 82.488 217 35 2 1 217 1 214 1.99e-127 362
MS.gene80793.t1 AT5G60660 68.302 265 73 4 32 288 16 277 2.84e-126 362
MS.gene80793.t1 AT2G39010 66.920 263 80 2 32 288 15 276 1.29e-125 360
MS.gene80793.t1 AT2G39010 64.894 188 59 2 32 213 15 201 1.48e-81 245
MS.gene80793.t1 AT3G16240 37.168 226 127 5 55 277 19 232 1.59e-31 118
MS.gene80793.t1 AT5G47450 37.662 231 129 7 50 277 14 232 1.10e-30 115
MS.gene80793.t1 AT4G01470 35.593 236 137 5 45 277 11 234 3.12e-30 114
MS.gene80793.t1 AT1G73190 37.083 240 130 7 55 285 24 251 4.14e-30 114
MS.gene80793.t1 AT1G17810 35.146 239 134 7 55 284 24 250 7.84e-30 114
MS.gene80793.t1 AT4G17340 36.283 226 129 6 55 277 19 232 4.41e-27 106
MS.gene80793.t1 AT2G36830 32.389 247 136 8 44 279 10 236 2.10e-26 104
MS.gene80793.t1 AT3G26520 33.188 229 132 6 55 277 22 235 1.24e-25 102
MS.gene80793.t1 AT2G25810 35.652 230 130 7 55 281 19 233 5.36e-24 98.2
MS.gene80793.t1 AT3G47440 31.687 243 135 8 50 281 18 240 1.32e-22 94.4
MS.gene80793.t1 AT1G31885 29.150 247 154 6 26 270 22 249 5.72e-20 88.6
MS.gene80793.t1 AT4G19030 27.985 268 175 7 26 288 26 280 3.32e-19 85.9
MS.gene80793.t1 AT5G37820 29.032 217 128 7 58 269 82 277 2.40e-16 78.2
MS.gene80793.t1 AT5G37820 29.032 217 128 7 58 269 46 241 2.48e-16 77.8
MS.gene80793.t1 AT1G31885 29.082 196 129 4 77 270 8 195 5.42e-16 76.6
MS.gene80793.t1 AT4G18910 27.509 269 175 8 26 287 21 276 7.02e-16 76.6
MS.gene80793.t1 AT2G34390 26.071 280 178 9 6 277 2 260 1.83e-15 75.5
MS.gene80793.t1 AT4G10380 24.901 253 158 6 33 270 41 276 1.33e-12 67.4
MS.gene80793.t1 AT5G37810 27.189 217 132 7 58 269 46 241 1.74e-12 66.6
MS.gene80793.t1 AT1G80760 25.301 249 166 6 32 280 60 288 2.40e-12 66.6
MS.gene80793.t1 AT2G34390 26.667 225 145 7 58 277 9 218 4.14e-12 65.1

Find 81 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCAAGTGGTTGTCTTTC+TGG 0.098743 5.3:+66424057 None:intergenic
CAAGGAATTGCTTGGGCTTT+TGG 0.147648 5.3:-66423832 MS.gene80793:CDS
TATTCTTGCAGTGGATCTTC+TGG 0.169058 5.3:-66422083 MS.gene80793:intron
TTAATACCAGTTCCAGTAAT+TGG 0.193062 5.3:+66422276 None:intergenic
TTCTGGGTGGGACCATTTAT+TGG 0.238352 5.3:-66422066 MS.gene80793:CDS
TTTGAAGGAAAGACAAGATT+TGG 0.252728 5.3:-66422695 MS.gene80793:CDS
CGCTGTGTTCTTGGTTCACT+TGG 0.261822 5.3:-66422307 MS.gene80793:CDS
GCAAGAGCTGCTCCAATAAA+TGG 0.306781 5.3:+66422054 None:intergenic
AATTGGGTTCGCTGTGTTCT+TGG 0.323972 5.3:-66422316 MS.gene80793:CDS
AAACAGTGATATAAAGAAAA+AGG 0.328096 5.3:+66423908 None:intergenic
ACAAGATTTGGAGATCTAAA+AGG 0.340417 5.3:-66422683 MS.gene80793:CDS
GGTGGCTCTTGATAGTCCTT+AGG 0.343217 5.3:+66424012 None:intergenic
ATAAATCCAGCTGTGACATT+TGG 0.343381 5.3:-66422815 MS.gene80793:CDS
TCCCAAGTGGTTGTCTTTCT+GGG 0.348961 5.3:+66424058 None:intergenic
CTACATGGTGATGCAATGTT+TGG 0.359716 5.3:-66422750 MS.gene80793:CDS
AGCCATCTGAACTGACTTCA+TGG 0.369669 5.3:-66423972 MS.gene80793:CDS
AAAGAACAGGATGTGTCTTT+AGG 0.370646 5.3:-66424093 MS.gene80793:CDS
ATTCTTGCAGTGGATCTTCT+GGG 0.373293 5.3:-66422082 MS.gene80793:intron
TAAATCCAGCTGTGACATTT+GGG 0.379708 5.3:-66422814 MS.gene80793:CDS
GATAGTCCTTAGGGTCATCT+TGG 0.381110 5.3:+66424022 None:intergenic
TACTGTACTGCTGGAATCTC+AGG 0.389203 5.3:-66423787 MS.gene80793:intron
TTGGGTGCTATTTGTGGTGC+TGG 0.389900 5.3:-66422731 MS.gene80793:CDS
TTGGCCACTATCCCAATTAC+TGG 0.392883 5.3:-66422288 MS.gene80793:CDS
GCTCTTGTTTACTGTACTGC+TGG 0.392947 5.3:-66423796 MS.gene80793:CDS
GTAACCAGGAGCAACAAAAT+TGG 0.398341 5.3:+66422655 None:intergenic
ATTTGATCACTTTGACTTGA+AGG 0.406054 5.3:+66421996 None:intergenic
CAATGTTTGGGTGCTATTTG+TGG 0.409953 5.3:-66422737 MS.gene80793:CDS
TAATACCAGTTCCAGTAATT+GGG 0.410603 5.3:+66422277 None:intergenic
GGGGATTCAAGGAATTGCTT+GGG 0.411864 5.3:-66423839 MS.gene80793:CDS
TGTAAGACTGTGGGGATTCA+AGG 0.414562 5.3:-66423850 MS.gene80793:CDS
ACTATCCCAATTACTGGAAC+TGG 0.426227 5.3:-66422282 MS.gene80793:CDS
AGCACCAAGACCATCACCTT+TGG 0.435976 5.3:+66422631 None:intergenic
TCATGGTCTTTCTACAGAGC+TGG 0.438821 5.3:-66423955 MS.gene80793:CDS
TGTAGAACAATGCTCTTGTC+AGG 0.441418 5.3:+66422768 None:intergenic
TGGGGATTCAAGGAATTGCT+TGG 0.444258 5.3:-66423840 MS.gene80793:CDS
GTGGCTCTTGATAGTCCTTA+GGG 0.463360 5.3:+66424013 None:intergenic
TACATGGTGATGCAATGTTT+GGG 0.464448 5.3:-66422749 MS.gene80793:CDS
ATTAACCCTGCTCGTAGTCT+TGG 0.478309 5.3:-66422258 MS.gene80793:CDS
TGTGGTGCTGGTGTTGTGAA+AGG 0.479519 5.3:-66422719 MS.gene80793:CDS
TCAAACAACGGTGCTGGTGG+TGG 0.481920 5.3:+66423994 None:intergenic
TCTTCAACAAGGACCTTGGT+TGG 0.486748 5.3:-66422224 MS.gene80793:CDS
GGTCTTGGTGCTGAAATTGT+TGG 0.490732 5.3:-66422620 MS.gene80793:CDS
TACGGGAACATGAGAGTCTC+TGG 0.492319 5.3:+66422544 None:intergenic
AAGAACACAGCGAACCCAAT+TGG 0.494251 5.3:+66422318 None:intergenic
AGAAATGGAAGCTAAAGAAC+AGG 0.501871 5.3:-66424106 None:intergenic
GATGGCTCAAACAACGGTGC+TGG 0.510215 5.3:+66423988 None:intergenic
AATAGCCCAAATGTCACAGC+TGG 0.512128 5.3:+66422809 None:intergenic
TCACTTTGACTTGAAGGGAA+TGG 0.512515 5.3:+66422002 None:intergenic
AGTTCCAGTAATTGGGATAG+TGG 0.513529 5.3:+66422284 None:intergenic
TTTGATCACTTTGACTTGAA+GGG 0.520518 5.3:+66421997 None:intergenic
AGTTCAGATGGCTCAAACAA+CGG 0.521397 5.3:+66423982 None:intergenic
ATTATCTTCAACAAGGACCT+TGG 0.525491 5.3:-66422228 MS.gene80793:CDS
GACCATGAAGTCAGTTCAGA+TGG 0.532853 5.3:+66423970 None:intergenic
TTGCAGTGGATCTTCTGGGT+GGG 0.533501 5.3:-66422078 MS.gene80793:intron
CATGGTCTTTCTACAGAGCT+GGG 0.538255 5.3:-66423954 MS.gene80793:CDS
GTTGCTCCTGGTTACACCAA+AGG 0.544188 5.3:-66422647 MS.gene80793:CDS
TACACCAAAGGTGATGGTCT+TGG 0.554514 5.3:-66422635 MS.gene80793:CDS
GATTCTAAGTGTAAGACTGT+GGG 0.554986 5.3:-66423859 MS.gene80793:CDS
CCTGGTTACACCAAAGGTGA+TGG 0.563447 5.3:-66422641 MS.gene80793:CDS
CCATCACCTTTGGTGTAACC+AGG 0.566415 5.3:+66422641 None:intergenic
AGCATCGGTGGCTGAGAAGA+CGG 0.570780 5.3:+66422577 None:intergenic
GGCGCTACGTTTAGCATCGG+TGG 0.574723 5.3:+66422565 None:intergenic
GCAGCACCAAGACTACGAGC+AGG 0.585086 5.3:+66422252 None:intergenic
TCAAAGCCAAGATGACCCTA+AGG 0.586870 5.3:-66424028 MS.gene80793:CDS
GACAAGAGCATTGTTCTACA+TGG 0.593377 5.3:-66422765 MS.gene80793:CDS
TCCCAGAAAGACAACCACTT+GGG 0.597140 5.3:-66424059 MS.gene80793:CDS
CTTGCAGTGGATCTTCTGGG+TGG 0.604626 5.3:-66422079 MS.gene80793:intron
CTTTGAGCTGCAATCCCAAG+TGG 0.615951 5.3:+66424045 None:intergenic
TTCCCAGAAAGACAACCACT+TGG 0.616137 5.3:-66424060 MS.gene80793:CDS
GTAGAACAATGCTCTTGTCA+GGG 0.621494 5.3:+66422769 None:intergenic
GGCTCAAACAACGGTGCTGG+TGG 0.625378 5.3:+66423991 None:intergenic
TGATTCTAAGTGTAAGACTG+TGG 0.635046 5.3:-66423860 MS.gene80793:CDS
ACAGCGAACCCAATTGGCAA+TGG 0.642498 5.3:+66422324 None:intergenic
TGCTGCTATTATCTTCAACA+AGG 0.649253 5.3:-66422235 MS.gene80793:CDS
ATTCTAAGTGTAAGACTGTG+GGG 0.650236 5.3:-66423858 MS.gene80793:CDS
TCTGGCGCTACGTTTAGCAT+CGG 0.662595 5.3:+66422562 None:intergenic
CAGCACCAAGACTACGAGCA+GGG 0.662928 5.3:+66422253 None:intergenic
AGATTTGGAGATCTAAAAGG+TGG 0.683343 5.3:-66422680 MS.gene80793:CDS
TTACATGATCATTCCAACCA+AGG 0.692776 5.3:+66422211 None:intergenic
TGGCTCTGATTACGACCACG+TGG 0.762119 5.3:+66422022 None:intergenic
TCTTGCAGCACTCTACCACG+TGG 0.776974 5.3:-66422037 MS.gene80793:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTTATAGTAATTTGA+TGG + chr5.3:66423615-66423634 None:intergenic 10.0%
!!! TATTTTTATTTTTCTGTTAT+AGG - chr5.3:66422709-66422728 MS.gene80793:CDS 10.0%
!! AATATAACTAATTATTTGTG+TGG - chr5.3:66423732-66423751 MS.gene80793:intron 15.0%
!! ACTTAATAACTAATTATTAC+AGG - chr5.3:66423706-66423725 MS.gene80793:intron 15.0%
!! AGTGAAACTTATATTTAATA+AGG - chr5.3:66423106-66423125 MS.gene80793:intron 15.0%
!! GTGAAACTTATATTTAATAA+GGG - chr5.3:66423107-66423126 MS.gene80793:intron 15.0%
!! TAAATAAATTTCATTCAAAC+TGG + chr5.3:66422758-66422777 None:intergenic 15.0%
!!! TTAAGTTTAAAATTATGCAT+TGG - chr5.3:66422559-66422578 MS.gene80793:CDS 15.0%
!!! TTAGTACTTATAAGTTTTTA+AGG - chr5.3:66422656-66422675 MS.gene80793:CDS 15.0%
!!! TTATATTAGTAGTTTTTTTG+TGG - chr5.3:66422870-66422889 MS.gene80793:intron 15.0%
!! AAACAGTGATATAAAGAAAA+AGG + chr5.3:66422199-66422218 None:intergenic 20.0%
!! AGTTATTAAGTGTATATTGT+CGG + chr5.3:66423697-66423716 None:intergenic 20.0%
!! ATAGTGCAAATATTCTAAAT+AGG + chr5.3:66422397-66422416 None:intergenic 20.0%
!! ATTAATTATTATTCTTGCAG+TGG - chr5.3:66424012-66424031 MS.gene80793:CDS 20.0%
!!! CTTATTTTATGAGTAGATTT+TGG - chr5.3:66423755-66423774 MS.gene80793:intron 20.0%
! AAATGCATCTAATGTTAGTT+TGG - chr5.3:66423196-66423215 MS.gene80793:intron 25.0%
! CCAAATTGACTAATAAAAGA+TGG + chr5.3:66423234-66423253 None:intergenic 25.0%
! CTAAATAGGTCAAGTAATAT+GGG + chr5.3:66422383-66422402 None:intergenic 25.0%
! TCAGATACTCGATATATAAT+TGG - chr5.3:66423661-66423680 MS.gene80793:intron 25.0%
! TCTAAATAGGTCAAGTAATA+TGG + chr5.3:66422384-66422403 None:intergenic 25.0%
! TTATGAATATGTCCTTATCA+TGG + chr5.3:66422977-66422996 None:intergenic 25.0%
!! CCATCTTTTATTAGTCAATT+TGG - chr5.3:66423231-66423250 MS.gene80793:intron 25.0%
AAATTACCAAAATGAGAGTG+TGG + chr5.3:66423057-66423076 None:intergenic 30.0%
AAGTAACAACTACCATGATA+AGG - chr5.3:66422962-66422981 MS.gene80793:intron 30.0%
ACAAGATTTGGAGATCTAAA+AGG - chr5.3:66423421-66423440 MS.gene80793:intron 30.0%
TAATACCAGTTCCAGTAATT+GGG + chr5.3:66423830-66423849 None:intergenic 30.0%
TCAACTAGGTCAAGTAATAT+GGG - chr5.3:66422596-66422615 MS.gene80793:CDS 30.0%
TGATGGTTATGATTGATTGA+CGG + chr5.3:66423598-66423617 None:intergenic 30.0%
TTAATACCAGTTCCAGTAAT+TGG + chr5.3:66423831-66423850 None:intergenic 30.0%
TTTGAAGGAAAGACAAGATT+TGG - chr5.3:66423409-66423428 MS.gene80793:intron 30.0%
! ACTCTTCTTAAGTAGTCTTT+TGG + chr5.3:66423160-66423179 None:intergenic 30.0%
! TTTCATTCAAACTGGTCTAT+AGG + chr5.3:66422750-66422769 None:intergenic 30.0%
!!! ATCACTGTTTTGACTGTTAT+GGG - chr5.3:66422209-66422228 MS.gene80793:intron 30.0%
!!! TATCACTGTTTTGACTGTTA+TGG - chr5.3:66422208-66422227 MS.gene80793:intron 30.0%
AAAGAACAGGATGTGTCTTT+AGG - chr5.3:66422011-66422030 MS.gene80793:CDS 35.0%
AGATTTGGAGATCTAAAAGG+TGG - chr5.3:66423424-66423443 MS.gene80793:intron 35.0%
ATAAATCCAGCTGTGACATT+TGG - chr5.3:66423289-66423308 MS.gene80793:intron 35.0%
ATAGGTCAAGTAATATGGGA+CGG + chr5.3:66422379-66422398 None:intergenic 35.0%
ATTATCTTCAACAAGGACCT+TGG - chr5.3:66423876-66423895 MS.gene80793:CDS 35.0%
ATTCTAAGTGTAAGACTGTG+GGG - chr5.3:66422246-66422265 MS.gene80793:CDS 35.0%
GATTCTAAGTGTAAGACTGT+GGG - chr5.3:66422245-66422264 MS.gene80793:CDS 35.0%
GTCAACTAGGTCAAGTAATA+TGG - chr5.3:66422595-66422614 MS.gene80793:CDS 35.0%
TAAATCCAGCTGTGACATTT+GGG - chr5.3:66423290-66423309 MS.gene80793:intron 35.0%
TGATTCTAAGTGTAAGACTG+TGG - chr5.3:66422244-66422263 MS.gene80793:CDS 35.0%
TGCTGCTATTATCTTCAACA+AGG - chr5.3:66423869-66423888 MS.gene80793:CDS 35.0%
TTACATGATCATTCCAACCA+AGG + chr5.3:66423896-66423915 None:intergenic 35.0%
! AAAGAACCACACTCTCATTT+TGG - chr5.3:66423048-66423067 MS.gene80793:intron 35.0%
! TACATGGTGATGCAATGTTT+GGG - chr5.3:66423355-66423374 MS.gene80793:intron 35.0%
!! TGTGACATTTGGGCTATTTT+TGG - chr5.3:66423300-66423319 MS.gene80793:intron 35.0%
ACTATCCCAATTACTGGAAC+TGG - chr5.3:66423822-66423841 MS.gene80793:CDS 40.0%
AGTTCAGATGGCTCAAACAA+CGG + chr5.3:66422125-66422144 None:intergenic 40.0%
AGTTCCAGTAATTGGGATAG+TGG + chr5.3:66423823-66423842 None:intergenic 40.0%
ATTCTTGCAGTGGATCTTCT+GGG - chr5.3:66424022-66424041 MS.gene80793:CDS 40.0%
CTAGGTCAAGTAATATGGGA+CGG - chr5.3:66422600-66422619 MS.gene80793:CDS 40.0%
GTAACCAGGAGCAACAAAAT+TGG + chr5.3:66423452-66423471 None:intergenic 40.0%
GTAGAACAATGCTCTTGTCA+GGG + chr5.3:66423338-66423357 None:intergenic 40.0%
TATTCTTGCAGTGGATCTTC+TGG - chr5.3:66424021-66424040 MS.gene80793:CDS 40.0%
TGACGGTGTAAAGAGACTTA+CGG + chr5.3:66423581-66423600 None:intergenic 40.0%
TGTAGAACAATGCTCTTGTC+AGG + chr5.3:66423339-66423358 None:intergenic 40.0%
! CTACATGGTGATGCAATGTT+TGG - chr5.3:66423354-66423373 MS.gene80793:intron 40.0%
! TGTTAGTGTCACTAGCACAT+AGG + chr5.3:66422943-66422962 None:intergenic 40.0%
! TTTTGGCACCATTGCCAATT+GGG - chr5.3:66423772-66423791 MS.gene80793:intron 40.0%
!! ATTTTGGCACCATTGCCAAT+TGG - chr5.3:66423771-66423790 MS.gene80793:intron 40.0%
!! CAATGTTTGGGTGCTATTTG+TGG - chr5.3:66423367-66423386 MS.gene80793:intron 40.0%
!! GACAAGAGCATTGTTCTACA+TGG - chr5.3:66423339-66423358 MS.gene80793:intron 40.0%
!! GGTGTTGTGAAAGGTTTTGA+AGG - chr5.3:66423394-66423413 MS.gene80793:intron 40.0%
!! TCACTTTGACTTGAAGGGAA+TGG + chr5.3:66424105-66424124 None:intergenic 40.0%
!!! CATTTGGGCTATTTTTGGCA+AGG - chr5.3:66423305-66423324 MS.gene80793:intron 40.0%
AACTATGCACTGTGTGATGC+AGG - chr5.3:66423259-66423278 MS.gene80793:intron 45.0%
AAGAACACAGCGAACCCAAT+TGG + chr5.3:66423789-66423808 None:intergenic 45.0%
AATAGCCCAAATGTCACAGC+TGG + chr5.3:66423298-66423317 None:intergenic 45.0%
AATTGGGTTCGCTGTGTTCT+TGG - chr5.3:66423788-66423807 MS.gene80793:CDS 45.0%
ATCCCAAGTGGTTGTCTTTC+TGG + chr5.3:66422050-66422069 None:intergenic 45.0%
ATTAACCCTGCTCGTAGTCT+TGG - chr5.3:66423846-66423865 MS.gene80793:CDS 45.0%
CATACGTGACAGAGTCAACT+AGG - chr5.3:66422582-66422601 MS.gene80793:CDS 45.0%
CATGGTCTTTCTACAGAGCT+GGG - chr5.3:66422150-66422169 MS.gene80793:intron 45.0%
GACCATGAAGTCAGTTCAGA+TGG + chr5.3:66422137-66422156 None:intergenic 45.0%
GACGGTGTAAAGAGACTTAC+GGG + chr5.3:66423580-66423599 None:intergenic 45.0%
GATAGTCCTTAGGGTCATCT+TGG + chr5.3:66422085-66422104 None:intergenic 45.0%
GCAAGAGCTGCTCCAATAAA+TGG + chr5.3:66424053-66424072 None:intergenic 45.0%
GGGGATTCAAGGAATTGCTT+GGG - chr5.3:66422265-66422284 MS.gene80793:CDS 45.0%
GTCAAGTAATATGGGACGGA+GGG - chr5.3:66422604-66422623 MS.gene80793:CDS 45.0%
GTCAAGTAATATGGGACGGA+GGG + chr5.3:66422623-66422604 None:intergenic 45.0%
TACTGTACTGCTGGAATCTC+AGG - chr5.3:66422317-66422336 MS.gene80793:CDS 45.0%
TCAAAGCCAAGATGACCCTA+AGG - chr5.3:66422076-66422095 MS.gene80793:intron 45.0%
TCATGGTCTTTCTACAGAGC+TGG - chr5.3:66422149-66422168 MS.gene80793:intron 45.0%
TCCCAAGTGGTTGTCTTTCT+GGG + chr5.3:66422049-66422068 None:intergenic 45.0%
TCCCAGAAAGACAACCACTT+GGG - chr5.3:66422045-66422064 MS.gene80793:CDS 45.0%
TCTTCAACAAGGACCTTGGT+TGG - chr5.3:66423880-66423899 MS.gene80793:CDS 45.0%
TGGGGATTCAAGGAATTGCT+TGG - chr5.3:66422264-66422283 MS.gene80793:CDS 45.0%
TGTAAGACTGTGGGGATTCA+AGG - chr5.3:66422254-66422273 MS.gene80793:CDS 45.0%
TTCCCAGAAAGACAACCACT+TGG - chr5.3:66422044-66422063 MS.gene80793:CDS 45.0%
TTCTGGGTGGGACCATTTAT+TGG - chr5.3:66424038-66424057 MS.gene80793:CDS 45.0%
TTGGCCACTATCCCAATTAC+TGG - chr5.3:66423816-66423835 MS.gene80793:CDS 45.0%
! AGCCATCTGAACTGACTTCA+TGG - chr5.3:66422132-66422151 MS.gene80793:intron 45.0%
! CAAGGAATTGCTTGGGCTTT+TGG - chr5.3:66422272-66422291 MS.gene80793:CDS 45.0%
! GCTCTTGTTTACTGTACTGC+TGG - chr5.3:66422308-66422327 MS.gene80793:CDS 45.0%
! GTGGCTCTTGATAGTCCTTA+GGG + chr5.3:66422094-66422113 None:intergenic 45.0%
! TACACCAAAGGTGATGGTCT+TGG - chr5.3:66423469-66423488 MS.gene80793:intron 45.0%
!! GGTCTTGGTGCTGAAATTGT+TGG - chr5.3:66423484-66423503 MS.gene80793:intron 45.0%
!! ATAAATAATAATTTGATATA+AGG - chr5.3:66423023-66423042 MS.gene80793:intron 5.0%
ACAGCGAACCCAATTGGCAA+TGG + chr5.3:66423783-66423802 None:intergenic 50.0%
AGCACCAAGACCATCACCTT+TGG + chr5.3:66423476-66423495 None:intergenic 50.0%
CCTGGTTACACCAAAGGTGA+TGG - chr5.3:66423463-66423482 MS.gene80793:intron 50.0%
CGCTGTGTTCTTGGTTCACT+TGG - chr5.3:66423797-66423816 MS.gene80793:CDS 50.0%
CTTTGAGCTGCAATCCCAAG+TGG + chr5.3:66422062-66422081 None:intergenic 50.0%
GGTCAAGTAATATGGGACGG+AGG - chr5.3:66422603-66422622 MS.gene80793:CDS 50.0%
GGTCAAGTAATATGGGACGG+AGG + chr5.3:66422622-66422603 None:intergenic 50.0%
GTTGCTCCTGGTTACACCAA+AGG - chr5.3:66423457-66423476 MS.gene80793:intron 50.0%
TACGGGAACATGAGAGTCTC+TGG + chr5.3:66423563-66423582 None:intergenic 50.0%
TATGCACTGTGTGATGCAGG+TGG - chr5.3:66423262-66423281 MS.gene80793:intron 50.0%
TCTGGCGCTACGTTTAGCAT+CGG + chr5.3:66423545-66423564 None:intergenic 50.0%
TTGCAGTGGATCTTCTGGGT+GGG - chr5.3:66424026-66424045 MS.gene80793:CDS 50.0%
! CCATCACCTTTGGTGTAACC+AGG + chr5.3:66423466-66423485 None:intergenic 50.0%
! GGAATTGCTTGGGCTTTTGG+TGG - chr5.3:66422275-66422294 MS.gene80793:CDS 50.0%
! GGTGCCAATTTTGTTGCTCC+TGG - chr5.3:66423445-66423464 MS.gene80793:intron 50.0%
! GGTGGCTCTTGATAGTCCTT+AGG + chr5.3:66422095-66422114 None:intergenic 50.0%
!! TGGGACGGAGGGAGTATTTA+AGG + chr5.3:66422364-66422383 None:intergenic 50.0%
!! TGTGGTGCTGGTGTTGTGAA+AGG - chr5.3:66423385-66423404 MS.gene80793:intron 50.0%
!! TTGGGTGCTATTTGTGGTGC+TGG - chr5.3:66423373-66423392 MS.gene80793:intron 50.0%
CAGCACCAAGACTACGAGCA+GGG + chr5.3:66423854-66423873 None:intergenic 55.0%
CTTGCAGTGGATCTTCTGGG+TGG - chr5.3:66424025-66424044 MS.gene80793:CDS 55.0%
GATGGCTCAAACAACGGTGC+TGG + chr5.3:66422119-66422138 None:intergenic 55.0%
TCTTGCAGCACTCTACCACG+TGG - chr5.3:66424067-66424086 MS.gene80793:CDS 55.0%
!! AGCATCGGTGGCTGAGAAGA+CGG + chr5.3:66423530-66423549 None:intergenic 55.0%
!! TCAAACAACGGTGCTGGTGG+TGG + chr5.3:66422113-66422132 None:intergenic 55.0%
!! TGGCTCTGATTACGACCACG+TGG + chr5.3:66424085-66424104 None:intergenic 55.0%
GCAGCACCAAGACTACGAGC+AGG + chr5.3:66423855-66423874 None:intergenic 60.0%
! GGCGCTACGTTTAGCATCGG+TGG + chr5.3:66423542-66423561 None:intergenic 60.0%
! GGCTCAAACAACGGTGCTGG+TGG + chr5.3:66422116-66422135 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 66422002 66424124 66422002 ID=MS.gene80793
chr5.3 mRNA 66422002 66424124 66422002 ID=MS.gene80793.t1;Parent=MS.gene80793
chr5.3 exon 66423788 66424124 66423788 ID=MS.gene80793.t1.exon1;Parent=MS.gene80793.t1
chr5.3 CDS 66423788 66424124 66423788 ID=cds.MS.gene80793.t1;Parent=MS.gene80793.t1
chr5.3 exon 66422547 66422845 66422547 ID=MS.gene80793.t1.exon2;Parent=MS.gene80793.t1
chr5.3 CDS 66422547 66422845 66422547 ID=cds.MS.gene80793.t1;Parent=MS.gene80793.t1
chr5.3 exon 66422215 66422355 66422215 ID=MS.gene80793.t1.exon3;Parent=MS.gene80793.t1
chr5.3 CDS 66422215 66422355 66422215 ID=cds.MS.gene80793.t1;Parent=MS.gene80793.t1
chr5.3 exon 66422002 66422094 66422002 ID=MS.gene80793.t1.exon4;Parent=MS.gene80793.t1
chr5.3 CDS 66422002 66422094 66422002 ID=cds.MS.gene80793.t1;Parent=MS.gene80793.t1
Gene Sequence

>MS.gene80793

ATGGAAGCTAAAGAACAGGATGTGTCTTTAGGAGCAAACAAATTCCCAGAAAGACAACCACTTGGGATTGCAGCTCAAAGCCAAGATGACCCTAAGGACTATCAAGAGCCACCACCAGCACCGTTGTTTGAGCCATCTGAACTGACTTCATGGTCTTTCTACAGAGCTGGGATAGCTGAGTTCGTTGCTACTTTCCTTTTTCTTTATATCACTGTTTTGACTGTTATGGGTGTTGTTAGAGCTGATTCTAAGTGTAAGACTGTGGGGATTCAAGGAATTGCTTGGGCTTTTGGTGGCATGATCTTTGCTCTTGTTTACTGTACTGCTGGAATCTCAGGTATTTTCATAGTTTCTATTTTCCTTAAATACTCCCTCCGTCCCATATTACTTGACCTATTTAGAATATTTGCACTATTCATGTAACATGCTTTGACTCTATTTTTTTACTAATATATAAAAACAAAATATAGCATATAAGATGTTGTTAGATTCGTCTCGATTAATATTTTCAAAATATTAAATTTTCGTAATTTTTTCTACTAGATTATTAAAGAAATTTAAGTTTAAAATTATGCATTGGCATACGTGACAGAGTCAACTAGGTCAAGTAATATGGGACGGAGGGAGTAACAACATATATGTAATAAATATTCTTTAGTACTTATAAGTTTTTAAGGTTAGTTCATTCATAAATTTTGTTCAATTTTTATTTTTATTTTTCTGTTATAGGACTAAATTAATTGTTCCTATAGACCAGTTTGAATGAAATTTATTTAGAGAATTAATTTAAGAAAGTGTAATTTTATAAAGATTAAAAACATATTTAATTCTTATAATTCTTTAAAAAAAAACTCTAAAAATAATAACGTTATATTAGTAGTTTTTTTGTGGAATTAAGATAAGCTCACATAATGTTAAATTAAAAGAATAGCATATAACCTATGTGCTAGTGACACTAACAAGTAACAACTACCATGATAAGGACATATTCATAATAATTTGATAAAATATTTCAACAAAAATAAATAATAATTTGATATAAGGAAAAAGAACCACACTCTCATTTTGGTAATTTTTTTTATTTTATTTTATGAAAAGTTTAATAGTGAAACTTATATTTAATAAGGGCAGAGAACATCGTTGTAGTCTCTGAATCCAAAAGACTACTTAAGAAGAGTGCATATTTACACACTTAAATGCATCTAATGTTAGTTTGGTCTTAATTGAATCCATCTTTTATTAGTCAATTTGGTCTAAAACTATGCACTGTGTGATGCAGGTGGTCATATAAATCCAGCTGTGACATTTGGGCTATTTTTGGCAAGGAAATTGTCCCTGACAAGAGCATTGTTCTACATGGTGATGCAATGTTTGGGTGCTATTTGTGGTGCTGGTGTTGTGAAAGGTTTTGAAGGAAAGACAAGATTTGGAGATCTAAAAGGTGGTGCCAATTTTGTTGCTCCTGGTTACACCAAAGGTGATGGTCTTGGTGCTGAAATTGTTGGCACTTTCATTCTTGTTTACACCGTCTTCTCAGCCACCGATGCTAAACGTAGCGCCAGAGACTCTCATGTTCCCGTAAGTCTCTTTACACCGTCAATCAATCATAACCATCAAATTACTATAAATATTTGACTTTAGTCGTAACTATTTTTATAGTCAGATACTCGATATATAATTGGTTGTGATTTACCGACAATATACACTTAATAACTAATTATTACAGGAATAATATAACTAATTATTTGTGTGGCTTATTTTATGAGTAGATTTTGGCACCATTGCCAATTGGGTTCGCTGTGTTCTTGGTTCACTTGGCCACTATCCCAATTACTGGAACTGGTATTAACCCTGCTCGTAGTCTTGGTGCTGCTATTATCTTCAACAAGGACCTTGGTTGGAATGATCATGTAAGTTGAAAAATTGAAATTAGTTATAAATTCGTATTGTTTTTGTAAAATTATATATTTATTTTTTTGAATTTTCTTATCTCATTTATCTTTTGTGTTCATTAATTATTATTCTTGCAGTGGATCTTCTGGGTGGGACCATTTATTGGAGCAGCTCTTGCAGCACTCTACCACGTGGTCGTAATCAGAGCCATTCCCTTCAAGTCAAAGTGA
Protein sequence

>MS.gene80793.t1

MEAKEQDVSLGANKFPERQPLGIAAQSQDDPKDYQEPPPAPLFEPSELTSWSFYRAGIAEFVATFLFLYITVLTVMGVVRADSKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFEGKTRFGDLKGGANFVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDLGWNDHWIFWVGPFIGAALAALYHVVVIRAIPFKSK