Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83133.t1 | XP_013466508.1 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 9.00E-95 | 356.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83133.t1 | Q6NLC8 | 45.8 | 201 | 95 | 5 | 1 | 188 | 1 | 200 | 1.1e-32 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83133.t1 | A0A072VEV4 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 6.5e-95 | 356.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050521 | MS.gene83133 | 0.837488 | 5.05E-57 | -1.69E-46 |
| MS.gene058015 | MS.gene83133 | 0.840355 | 9.14E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83133 | MS.gene04894 | PPI |
| MS.gene83133 | MS.gene008202 | PPI |
| MS.gene000732 | MS.gene83133 | PPI |
| MS.gene29468 | MS.gene83133 | PPI |
| MS.gene63347 | MS.gene83133 | PPI |
| MS.gene62235 | MS.gene83133 | PPI |
| MS.gene017455 | MS.gene83133 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83133.t1 | MTR_1g030740 | 96.354 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 1.64e-133 | 372 |
| MS.gene83133.t1 | MTR_4g085100 | 70.857 | 175 | 43 | 4 | 1 | 170 | 1 | 172 | 5.85e-77 | 229 |
| MS.gene83133.t1 | MTR_5g079610 | 54.070 | 172 | 69 | 4 | 1 | 164 | 1 | 170 | 1.64e-55 | 175 |
| MS.gene83133.t1 | MTR_3g067730 | 49.746 | 197 | 84 | 4 | 1 | 182 | 1 | 197 | 1.03e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83133.t1 | AT2G01340 | 63.529 | 170 | 57 | 3 | 1 | 169 | 1 | 166 | 4.20e-64 | 197 |
| MS.gene83133.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 8.94e-49 | 157 |
| MS.gene83133.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 8.94e-49 | 157 |
| MS.gene83133.t1 | AT1G66480 | 50.000 | 176 | 74 | 4 | 1 | 163 | 1 | 175 | 1.96e-46 | 152 |
| MS.gene83133.t1 | AT5G37840 | 46.746 | 169 | 80 | 5 | 1 | 163 | 1 | 165 | 2.94e-39 | 134 |
Find 40 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAATAATGGGAAATAGTTT+AGG | 0.332015 | 1.2:+16191357 | None:intergenic |
| TCTGAGGAAGTGAAGCATTA+TGG | 0.340674 | 1.2:+16191492 | MS.gene83133:CDS |
| TAATGATAAGAAAATGAAAA+AGG | 0.345766 | 1.2:+16191863 | MS.gene83133:CDS |
| CCCACCTCGAAGTCGTTCAT+TGG | 0.353620 | 1.2:-16191702 | None:intergenic |
| TCTTGTAACTCGACTTTCCT+TGG | 0.360185 | 1.2:-16191586 | None:intergenic |
| AACTTCTCAACATCTGCCTT+TGG | 0.375562 | 1.2:-16191771 | None:intergenic |
| AACTCTTTGTGTGCTTCCAA+TGG | 0.426840 | 1.2:-16191528 | None:intergenic |
| GAAAGCCGGTGAAGTGCTTA+AGG | 0.427598 | 1.2:+16191443 | MS.gene83133:CDS |
| GTTACAAGAAGGGTTCGTTC+GGG | 0.451959 | 1.2:+16191600 | MS.gene83133:CDS |
| CTTGTAACTCGACTTTCCTT+GGG | 0.453744 | 1.2:-16191585 | None:intergenic |
| AGTTACAAGAAGGGTTCGTT+CGG | 0.464996 | 1.2:+16191599 | MS.gene83133:CDS |
| AAGAAGAGTTGACGAATGGC+GGG | 0.488902 | 1.2:+16191727 | MS.gene83133:CDS |
| AGCACTTCACCGGCTTTCAC+CGG | 0.493553 | 1.2:-16191438 | None:intergenic |
| GTGATCCTTAAGCACTTCAC+CGG | 0.518764 | 1.2:-16191448 | None:intergenic |
| ACAAAGGTGATGAAAATTGA+TGG | 0.530975 | 1.2:+16191393 | MS.gene83133:CDS |
| TCATTGGTTTCATAATCGTA+AGG | 0.535977 | 1.2:-16191686 | None:intergenic |
| AGTTGACGAATGGCGGGGTG+AGG | 0.542180 | 1.2:+16191733 | MS.gene83133:CDS |
| CTAAAGACACCGGTGAAAGC+CGG | 0.542691 | 1.2:+16191429 | MS.gene83133:CDS |
| TCTTGTCTTGTTAGAATCTG+AGG | 0.544333 | 1.2:+16191476 | MS.gene83133:CDS |
| TGGAAGGGTAGCAGAGGAGT+TGG | 0.545103 | 1.2:+16191894 | MS.gene83133:CDS |
| GAAACCAATGAACGACTTCG+AGG | 0.561915 | 1.2:+16191698 | MS.gene83133:CDS |
| AGGAAAGTCGAGTTACAAGA+AGG | 0.564448 | 1.2:+16191589 | MS.gene83133:CDS |
| ATAATGGGAAATAGTTTAGG+TGG | 0.567511 | 1.2:+16191360 | None:intergenic |
| GAAGATTATGAGTCTTTGCA+TGG | 0.572826 | 1.2:+16191830 | MS.gene83133:CDS |
| TGGAGTGAGAGCGAAGCCAT+TGG | 0.574068 | 1.2:+16191512 | MS.gene83133:CDS |
| AAAGAAGAGTTGACGAATGG+CGG | 0.576273 | 1.2:+16191726 | MS.gene83133:CDS |
| GATTCTAACAAGACAAGACC+AGG | 0.591028 | 1.2:-16191471 | None:intergenic |
| ACCAATGAACGACTTCGAGG+TGG | 0.613366 | 1.2:+16191701 | MS.gene83133:CDS |
| GATGCAAAGTAGTAAAGATG+AGG | 0.620365 | 1.2:+16191794 | MS.gene83133:CDS |
| AGAGTCTGTCTTTAGCTCGA+AGG | 0.625951 | 1.2:+16191652 | MS.gene83133:CDS |
| CCAATGAACGACTTCGAGGT+GGG | 0.626451 | 1.2:+16191702 | MS.gene83133:CDS |
| TTACAAGAAGGGTTCGTTCG+GGG | 0.631852 | 1.2:+16191601 | MS.gene83133:CDS |
| ATGCATTGGAAGGGTAGCAG+AGG | 0.643927 | 1.2:+16191888 | MS.gene83133:CDS |
| GGAAAGTCGAGTTACAAGAA+GGG | 0.646808 | 1.2:+16191590 | MS.gene83133:CDS |
| GGAAAAGAAGAGTTGACGAA+TGG | 0.648290 | 1.2:+16191723 | MS.gene83133:CDS |
| AGAAGAGTTGACGAATGGCG+GGG | 0.660824 | 1.2:+16191728 | MS.gene83133:CDS |
| GTTGAAAATGAGACTTCCAA+AGG | 0.662765 | 1.2:+16191755 | MS.gene83133:CDS |
| AACATTCAAGCTAAAGACAC+CGG | 0.668856 | 1.2:+16191419 | MS.gene83133:CDS |
| ATTCTAACAAGACAAGACCA+GGG | 0.767432 | 1.2:-16191470 | None:intergenic |
| GAAGTGCTTAAGGATCACCC+TGG | 0.784850 | 1.2:+16191453 | MS.gene83133:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATAAGAAAATGAAAA+AGG | + | chr1.2:16191863-16191882 | MS.gene83133:CDS | 15.0% |
| ! | AAATGAAAAAGGAAATGCAT+TGG | + | chr1.2:16191874-16191893 | MS.gene83133:CDS | 25.0% |
| !! | AAAAAGACTCTATTTTCTTG+TGG | + | chr1.2:16191557-16191576 | MS.gene83133:CDS | 25.0% |
| !! | ACAAGAAAATAGAGTCTTTT+TGG | - | chr1.2:16191558-16191577 | None:intergenic | 25.0% |
| !! | TGAATACAAAAAAGCTTTTG+AGG | + | chr1.2:16191917-16191936 | MS.gene83133:CDS | 25.0% |
| AAAAAGGAAATGCATTGGAA+GGG | + | chr1.2:16191879-16191898 | MS.gene83133:CDS | 30.0% | |
| ACAAAGGTGATGAAAATTGA+TGG | + | chr1.2:16191393-16191412 | MS.gene83133:CDS | 30.0% | |
| AGGTGGAAAAAAATCTACAA+AGG | + | chr1.2:16191377-16191396 | MS.gene83133:CDS | 30.0% | |
| !! | TCATTGGTTTCATAATCGTA+AGG | - | chr1.2:16191689-16191708 | None:intergenic | 30.0% |
| AACATTCAAGCTAAAGACAC+CGG | + | chr1.2:16191419-16191438 | MS.gene83133:CDS | 35.0% | |
| ATTCTAACAAGACAAGACCA+GGG | - | chr1.2:16191473-16191492 | None:intergenic | 35.0% | |
| GAAAAAGGAAATGCATTGGA+AGG | + | chr1.2:16191878-16191897 | MS.gene83133:CDS | 35.0% | |
| GAAGATTATGAGTCTTTGCA+TGG | + | chr1.2:16191830-16191849 | MS.gene83133:CDS | 35.0% | |
| GATGCAAAGTAGTAAAGATG+AGG | + | chr1.2:16191794-16191813 | MS.gene83133:CDS | 35.0% | |
| GTTGAAAATGAGACTTCCAA+AGG | + | chr1.2:16191755-16191774 | MS.gene83133:CDS | 35.0% | |
| ! | TCTTGTCTTGTTAGAATCTG+AGG | + | chr1.2:16191476-16191495 | MS.gene83133:CDS | 35.0% |
| AAAGAAGAGTTGACGAATGG+CGG | + | chr1.2:16191726-16191745 | MS.gene83133:CDS | 40.0% | |
| AACTCTTTGTGTGCTTCCAA+TGG | - | chr1.2:16191531-16191550 | None:intergenic | 40.0% | |
| AACTTCTCAACATCTGCCTT+TGG | - | chr1.2:16191774-16191793 | None:intergenic | 40.0% | |
| AGGAAAGTCGAGTTACAAGA+AGG | + | chr1.2:16191589-16191608 | MS.gene83133:CDS | 40.0% | |
| AGTTACAAGAAGGGTTCGTT+CGG | + | chr1.2:16191599-16191618 | MS.gene83133:CDS | 40.0% | |
| CTTGTAACTCGACTTTCCTT+GGG | - | chr1.2:16191588-16191607 | None:intergenic | 40.0% | |
| GATTCTAACAAGACAAGACC+AGG | - | chr1.2:16191474-16191493 | None:intergenic | 40.0% | |
| GGAAAAGAAGAGTTGACGAA+TGG | + | chr1.2:16191723-16191742 | MS.gene83133:CDS | 40.0% | |
| GGAAAGTCGAGTTACAAGAA+GGG | + | chr1.2:16191590-16191609 | MS.gene83133:CDS | 40.0% | |
| TCTTGTAACTCGACTTTCCT+TGG | - | chr1.2:16191589-16191608 | None:intergenic | 40.0% | |
| ! | TCTGAGGAAGTGAAGCATTA+TGG | + | chr1.2:16191492-16191511 | MS.gene83133:CDS | 40.0% |
| ! | TTTTCTTGTGGAACTTCCCA+AGG | + | chr1.2:16191569-16191588 | MS.gene83133:CDS | 40.0% |
| AAGAAGAGTTGACGAATGGC+GGG | + | chr1.2:16191727-16191746 | MS.gene83133:CDS | 45.0% | |
| GAAACCAATGAACGACTTCG+AGG | + | chr1.2:16191698-16191717 | MS.gene83133:CDS | 45.0% | |
| GTGATCCTTAAGCACTTCAC+CGG | - | chr1.2:16191451-16191470 | None:intergenic | 45.0% | |
| GTTACAAGAAGGGTTCGTTC+GGG | + | chr1.2:16191600-16191619 | MS.gene83133:CDS | 45.0% | |
| TTACAAGAAGGGTTCGTTCG+GGG | + | chr1.2:16191601-16191620 | MS.gene83133:CDS | 45.0% | |
| ! | AGAGTCTGTCTTTAGCTCGA+AGG | + | chr1.2:16191652-16191671 | MS.gene83133:CDS | 45.0% |
| ACCAATGAACGACTTCGAGG+TGG | + | chr1.2:16191701-16191720 | MS.gene83133:CDS | 50.0% | |
| AGAAGAGTTGACGAATGGCG+GGG | + | chr1.2:16191728-16191747 | MS.gene83133:CDS | 50.0% | |
| ATGCATTGGAAGGGTAGCAG+AGG | + | chr1.2:16191888-16191907 | MS.gene83133:CDS | 50.0% | |
| CCAATGAACGACTTCGAGGT+GGG | + | chr1.2:16191702-16191721 | MS.gene83133:CDS | 50.0% | |
| CTAAAGACACCGGTGAAAGC+CGG | + | chr1.2:16191429-16191448 | MS.gene83133:CDS | 50.0% | |
| ! | GAAAGCCGGTGAAGTGCTTA+AGG | + | chr1.2:16191443-16191462 | MS.gene83133:CDS | 50.0% |
| ! | GAAGTGCTTAAGGATCACCC+TGG | + | chr1.2:16191453-16191472 | MS.gene83133:CDS | 50.0% |
| CCCACCTCGAAGTCGTTCAT+TGG | - | chr1.2:16191705-16191724 | None:intergenic | 55.0% | |
| TGGAAGGGTAGCAGAGGAGT+TGG | + | chr1.2:16191894-16191913 | MS.gene83133:CDS | 55.0% | |
| TGGAGTGAGAGCGAAGCCAT+TGG | + | chr1.2:16191512-16191531 | MS.gene83133:CDS | 55.0% | |
| !! | AGCACTTCACCGGCTTTCAC+CGG | - | chr1.2:16191441-16191460 | None:intergenic | 55.0% |
| ! | AGTTGACGAATGGCGGGGTG+AGG | + | chr1.2:16191733-16191752 | MS.gene83133:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 16191363 | 16191944 | 16191363 | ID=MS.gene83133 |
| chr1.2 | mRNA | 16191363 | 16191944 | 16191363 | ID=MS.gene83133.t1;Parent=MS.gene83133 |
| chr1.2 | exon | 16191363 | 16191944 | 16191363 | ID=MS.gene83133.t1.exon1;Parent=MS.gene83133.t1 |
| chr1.2 | CDS | 16191363 | 16191944 | 16191363 | ID=cds.MS.gene83133.t1;Parent=MS.gene83133.t1 |
| Gene Sequence |
| Protein sequence |
