Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83688.t1 | XP_003612495.2 | 95.9 | 370 | 15 | 0 | 9 | 378 | 17 | 386 | 1.10E-189 | 672.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83688.t1 | O65709 | 48.5 | 307 | 146 | 3 | 72 | 378 | 1 | 295 | 2.5e-73 | 277.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83688.t1 | G7K421 | 95.9 | 370 | 15 | 0 | 9 | 378 | 17 | 386 | 8.0e-190 | 672.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049488 | MS.gene83688 | 0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene83688 | 0.810646 | 1.04E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene83688 | 0.811761 | 5.96E-51 | -1.69E-46 |
| MS.gene052346 | MS.gene83688 | 0.827342 | 1.65E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene83688 | 0.813075 | 3.08E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene83688 | 0.826537 | 2.57E-54 | -1.69E-46 |
| MS.gene053758 | MS.gene83688 | 0.801929 | 7.14E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene83688 | 0.809809 | 1.58E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene83688 | 0.840039 | 1.10E-57 | -1.69E-46 |
| MS.gene055911 | MS.gene83688 | 0.84111 | 5.80E-58 | -1.69E-46 |
| MS.gene056422 | MS.gene83688 | 0.810612 | 1.06E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene83688 | 0.840546 | 8.15E-58 | -1.69E-46 |
| MS.gene057076 | MS.gene83688 | 0.806533 | 7.86E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene83688 | 0.812409 | 4.30E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene83688 | 0.809679 | 1.68E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene83688 | 0.814973 | 1.17E-51 | -1.69E-46 |
| MS.gene059368 | MS.gene83688 | 0.8063 | 8.80E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene83688 | 0.810988 | 8.77E-51 | -1.69E-46 |
| MS.gene059807 | MS.gene83688 | 0.81594 | 7.14E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83688.t1 | MTR_5g025650 | 95.989 | 374 | 15 | 0 | 5 | 378 | 13 | 386 | 0.0 | 714 |
| MS.gene83688.t1 | MTR_8g072210 | 62.599 | 377 | 134 | 3 | 3 | 378 | 1 | 371 | 4.74e-137 | 410 |
| MS.gene83688.t1 | MTR_0651s0010 | 37.805 | 328 | 167 | 7 | 56 | 378 | 12 | 307 | 9.44e-54 | 188 |
| MS.gene83688.t1 | MTR_4g080823 | 37.195 | 328 | 169 | 7 | 56 | 378 | 21 | 316 | 5.31e-52 | 185 |
| MS.gene83688.t1 | MTR_4g080890 | 37.195 | 328 | 169 | 7 | 56 | 378 | 12 | 307 | 5.11e-51 | 182 |
| MS.gene83688.t1 | MTR_7g097210 | 34.328 | 335 | 177 | 8 | 48 | 378 | 7 | 302 | 9.85e-37 | 141 |
| MS.gene83688.t1 | MTR_1g075650 | 30.058 | 173 | 74 | 5 | 207 | 378 | 3 | 129 | 9.14e-14 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83688.t1 | AT4G19380 | 45.977 | 348 | 175 | 4 | 32 | 378 | 2 | 337 | 1.08e-81 | 265 |
| MS.gene83688.t1 | AT4G19380 | 48.534 | 307 | 146 | 3 | 72 | 378 | 1 | 295 | 1.55e-76 | 250 |
| MS.gene83688.t1 | AT4G28570 | 32.407 | 324 | 186 | 5 | 57 | 378 | 17 | 309 | 1.54e-34 | 135 |
| MS.gene83688.t1 | AT4G28570 | 32.407 | 324 | 186 | 5 | 57 | 378 | 17 | 309 | 1.54e-34 | 135 |
| MS.gene83688.t1 | AT1G03990 | 29.787 | 329 | 191 | 6 | 59 | 378 | 20 | 317 | 2.87e-30 | 122 |
| MS.gene83688.t1 | AT3G23410 | 30.368 | 326 | 177 | 9 | 67 | 378 | 15 | 304 | 5.35e-30 | 122 |
| MS.gene83688.t1 | AT3G23410 | 32.830 | 265 | 139 | 7 | 119 | 378 | 6 | 236 | 1.98e-26 | 111 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAACTGAGCTTTGAATTC+TGG | 0.175339 | 5.4:+16746223 | None:intergenic |
| TCAGGGAAAGTGTGTATAAA+AGG | 0.217807 | 5.4:+16754495 | None:intergenic |
| TGGTTTCTGTCTACATGGTT+AGG | 0.272649 | 5.4:-16754561 | MS.gene83688:CDS |
| TTGTTCTGAACAACATTCTA+AGG | 0.279704 | 5.4:+16754412 | None:intergenic |
| ATGAATATCTTCATATTATC+AGG | 0.322533 | 5.4:-16745791 | MS.gene83688:CDS |
| TGGAGTTGTAGCCGGAATTC+TGG | 0.330538 | 5.4:-16745942 | MS.gene83688:CDS |
| AACTTTAGTACCATTGTCAT+TGG | 0.342432 | 5.4:+16754591 | None:intergenic |
| ACCGGCATTTGCCAGAATTC+CGG | 0.343046 | 5.4:+16745931 | None:intergenic |
| GTCGACACTCTAAGACGATT+TGG | 0.360914 | 5.4:-16746073 | MS.gene83688:CDS |
| TGGTACTAAAGTTGGTGTTA+TGG | 0.374436 | 5.4:-16754581 | MS.gene83688:CDS |
| TTTCGACGGGTAACCGAGAC+AGG | 0.374753 | 5.4:+16746046 | None:intergenic |
| CGTTTGTGTAGAGGTAAATC+AGG | 0.376447 | 5.4:+16754477 | None:intergenic |
| ATGGGAAAATATACACGTTC+AGG | 0.388465 | 5.4:+16755404 | None:intergenic |
| AATCAGAAGACAATTGTTCA+TGG | 0.394390 | 5.4:-16746273 | MS.gene83688:CDS |
| AACAATCTTTCTCTTCTTGA+AGG | 0.398166 | 5.4:-16745866 | MS.gene83688:CDS |
| AATTGTTCATGGAAAGCAAT+TGG | 0.401562 | 5.4:-16746262 | MS.gene83688:CDS |
| CGTATTTCAGCTTCCATGGC+TGG | 0.407420 | 5.4:-16755431 | MS.gene83688:CDS |
| GTGGTAGTTGGTTCCGGCTC+AGG | 0.417463 | 5.4:-16745968 | MS.gene83688:CDS |
| TAGAAAGGATAGTTTCAGAT+TGG | 0.419331 | 5.4:-16755675 | MS.gene83688:CDS |
| CAAAACCAACACTTTGTAAC+CGG | 0.424473 | 5.4:+16745913 | None:intergenic |
| CGGTTACAAAGTGTTGGTTT+TGG | 0.424634 | 5.4:-16745912 | MS.gene83688:CDS |
| GACGCTGTGGTAGTTGGTTC+CGG | 0.429362 | 5.4:-16745974 | MS.gene83688:CDS |
| GGTTTCTGTCTACATGGTTA+GGG | 0.431876 | 5.4:-16754560 | MS.gene83688:CDS |
| GGAAGCTGAAATACGGTAGA+AGG | 0.433466 | 5.4:+16755439 | None:intergenic |
| GCCAACAGATTATGCAATCT+TGG | 0.434426 | 5.4:-16754455 | MS.gene83688:CDS |
| AATGTACCAATCTGGCGGTT+TGG | 0.443491 | 5.4:-16745828 | MS.gene83688:CDS |
| AGAAAGGATAGTTTCAGATT+GGG | 0.448247 | 5.4:-16755674 | MS.gene83688:CDS |
| CTACCGTATTTCAGCTTCCA+TGG | 0.449137 | 5.4:-16755435 | MS.gene83688:CDS |
| TTACCTGAGTGAAGAAGATT+AGG | 0.450249 | 5.4:+16754373 | None:intergenic |
| CCCTTTGTAAAGAGGTCCTA+TGG | 0.460808 | 5.4:+16746129 | None:intergenic |
| GTTGGTTCCGGCTCAGGAGG+TGG | 0.462813 | 5.4:-16745962 | MS.gene83688:CDS |
| GGAACAAAGTCATTGATAGA+AGG | 0.463513 | 5.4:+16755491 | None:intergenic |
| TAGGAAGGGTAGCTTTAGAA+AGG | 0.469277 | 5.4:-16755690 | MS.gene83688:CDS |
| GACATGGAGAATAGTAATAG+TGG | 0.471196 | 5.4:-16755737 | MS.gene83688:CDS |
| AATGACAATGGTACTAAAGT+TGG | 0.471554 | 5.4:-16754589 | MS.gene83688:CDS |
| GATTCGTCGTCGTCGGAGAC+AGG | 0.475074 | 5.4:+16755470 | None:intergenic |
| TCACAAACTCTCTTTCTGTT+AGG | 0.475121 | 5.4:-16755553 | MS.gene83688:CDS |
| TGTTATGGTTTCTGTCTACA+TGG | 0.479156 | 5.4:-16754566 | MS.gene83688:CDS |
| TGAGCTTTGAATTCTGGATC+AGG | 0.482943 | 5.4:+16746229 | None:intergenic |
| ATGGATCAAATGTACCAATC+TGG | 0.485800 | 5.4:-16745836 | MS.gene83688:CDS |
| CAGGGAAAGTGTGTATAAAA+GGG | 0.494879 | 5.4:+16754496 | None:intergenic |
| CCATAGGACCTCTTTACAAA+GGG | 0.499145 | 5.4:-16746129 | MS.gene83688:CDS |
| TCACATTGTATAACTAAAGA+AGG | 0.507906 | 5.4:+16745995 | None:intergenic |
| TGGTACATTTGATCCATTGT+TGG | 0.511444 | 5.4:+16745842 | None:intergenic |
| AAAGAAGGAGATGATAAGCT+TGG | 0.514270 | 5.4:+16746010 | None:intergenic |
| GTGTATAAAAGGGAACTTTG+TGG | 0.515128 | 5.4:+16754506 | None:intergenic |
| TCTTCTTGAAGGACCAACAA+TGG | 0.515800 | 5.4:-16745855 | MS.gene83688:CDS |
| TACAACTCCACCTCCTGAGC+CGG | 0.517239 | 5.4:+16745955 | None:intergenic |
| TTGCAACCAAACCGCCAGAT+TGG | 0.521706 | 5.4:+16745822 | None:intergenic |
| CAGCCATGGAAGCTGAAATA+CGG | 0.530516 | 5.4:+16755432 | None:intergenic |
| ATCTGTTGGCGTTTGTGTAG+AGG | 0.531304 | 5.4:+16754468 | None:intergenic |
| GCCGGAATTCTGGCAAATGC+CGG | 0.537102 | 5.4:-16745932 | MS.gene83688:CDS |
| AAATGCCGGTTACAAAGTGT+TGG | 0.540631 | 5.4:-16745918 | MS.gene83688:CDS |
| TCAGGAGGTGGAGTTGTAGC+CGG | 0.545470 | 5.4:-16745950 | MS.gene83688:CDS |
| AAGGATAGTTTCAGATTGGG+AGG | 0.546950 | 5.4:-16755671 | MS.gene83688:CDS |
| ACGTTCAGGTGTTCCAGCCA+TGG | 0.550679 | 5.4:+16755418 | None:intergenic |
| CAATGTGACGCTGTGGTAGT+TGG | 0.551132 | 5.4:-16745980 | MS.gene83688:CDS |
| TGGACAAGCCCTTTGTAAAG+AGG | 0.551242 | 5.4:+16746121 | None:intergenic |
| ACCAAGATTGCATAATCTGT+TGG | 0.551313 | 5.4:+16754454 | None:intergenic |
| GATCACACAGTGGTTGAGCT+AGG | 0.554204 | 5.4:-16755644 | MS.gene83688:CDS |
| ACGATTTGGATTCCCTGTCT+CGG | 0.555886 | 5.4:-16746059 | MS.gene83688:CDS |
| AGTATCTGAGAGAGCAGTGA+GGG | 0.572897 | 5.4:+16755520 | None:intergenic |
| GTGTCGACAGTGATGTCTCT+CGG | 0.579082 | 5.4:+16746088 | None:intergenic |
| AGAAAGGAGGGTATTCAGCT+AGG | 0.580570 | 5.4:-16745889 | MS.gene83688:CDS |
| GCCATAGGACCTCTTTACAA+AGG | 0.583066 | 5.4:-16746130 | MS.gene83688:CDS |
| TGTCTCTCGGATAGTTGAGA+TGG | 0.589798 | 5.4:+16746101 | None:intergenic |
| GTTGTTTATGTGCAGCTTGG+GGG | 0.591555 | 5.4:-16754639 | MS.gene83688:intron |
| AAAAGTGGTAGCTTTAGGAA+GGG | 0.598113 | 5.4:-16755704 | MS.gene83688:CDS |
| GTAGTTGGTTCCGGCTCAGG+AGG | 0.605970 | 5.4:-16745965 | MS.gene83688:CDS |
| ATTATAGAGCAACATGGACA+TGG | 0.609231 | 5.4:-16755753 | None:intergenic |
| TCTCCTAATCTTCTTCACTC+AGG | 0.614451 | 5.4:-16754376 | MS.gene83688:intron |
| TGGAAAGCAATTGGATACTG+TGG | 0.629782 | 5.4:-16746253 | MS.gene83688:CDS |
| AGCAGCGGATTCGTCGTCGT+CGG | 0.630964 | 5.4:+16755463 | None:intergenic |
| TTCGACGGGTAACCGAGACA+GGG | 0.655689 | 5.4:+16746047 | None:intergenic |
| GTTTGTGTAGAGGTAAATCA+GGG | 0.659201 | 5.4:+16754478 | None:intergenic |
| AGTTATACAATGTGACGCTG+TGG | 0.659608 | 5.4:-16745987 | MS.gene83688:CDS |
| ACTGATTCATCCAATGACAA+TGG | 0.676312 | 5.4:-16754601 | MS.gene83688:CDS |
| GATGATGCTGAACAAGCCAT+AGG | 0.683975 | 5.4:-16746145 | MS.gene83688:CDS |
| GATCAAATGTACCAATCTGG+CGG | 0.687871 | 5.4:-16745833 | MS.gene83688:CDS |
| TAGTATCTGAGAGAGCAGTG+AGG | 0.721002 | 5.4:+16755519 | None:intergenic |
| AATACGGTAGAAGGTAGCAG+CGG | 0.736624 | 5.4:+16755448 | None:intergenic |
| GGGAGGAAAAGATCACACAG+TGG | 0.818391 | 5.4:-16755654 | MS.gene83688:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 16745784 | 16755762 | 16745784 | ID=MS.gene83688 |
| chr5.4 | mRNA | 16745784 | 16755762 | 16745784 | ID=MS.gene83688.t1;Parent=MS.gene83688 |
| chr5.4 | exon | 16755418 | 16755762 | 16755418 | ID=MS.gene83688.t1.exon1;Parent=MS.gene83688.t1 |
| chr5.4 | CDS | 16755418 | 16755762 | 16755418 | ID=cds.MS.gene83688.t1;Parent=MS.gene83688.t1 |
| chr5.4 | exon | 16754377 | 16754646 | 16754377 | ID=MS.gene83688.t1.exon2;Parent=MS.gene83688.t1 |
| chr5.4 | CDS | 16754377 | 16754646 | 16754377 | ID=cds.MS.gene83688.t1;Parent=MS.gene83688.t1 |
| chr5.4 | exon | 16745784 | 16746302 | 16745784 | ID=MS.gene83688.t1.exon3;Parent=MS.gene83688.t1 |
| chr5.4 | CDS | 16745784 | 16746302 | 16745784 | ID=cds.MS.gene83688.t1;Parent=MS.gene83688.t1 |
| Gene Sequence |
| Protein sequence |
