Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84001.t1 | PNX77523.1 | 60.3 | 78 | 21 | 2 | 1 | 76 | 1 | 70 | 4.30E-13 | 84.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84001.t1 | A0A392NFU7 | 50.0 | 124 | 47 | 3 | 1 | 116 | 1 | 117 | 6.3e-14 | 86.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene84001 | 0.809245 | 2.08E-50 | -1.69E-46 |
| MS.gene049587 | MS.gene84001 | 0.802995 | 4.30E-49 | -1.69E-46 |
| MS.gene049853 | MS.gene84001 | 0.836864 | 7.29E-57 | -1.69E-46 |
| MS.gene054174 | MS.gene84001 | 0.854866 | 9.25E-62 | -1.69E-46 |
| MS.gene054175 | MS.gene84001 | 0.859316 | 4.50E-63 | -1.69E-46 |
| MS.gene05435 | MS.gene84001 | 0.810031 | 1.41E-50 | -1.69E-46 |
| MS.gene054484 | MS.gene84001 | 0.8144 | 1.57E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene84001 | 0.808247 | 3.40E-50 | -1.69E-46 |
| MS.gene055058 | MS.gene84001 | 0.802974 | 4.35E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene84001 | 0.813382 | 2.63E-51 | -1.69E-46 |
| MS.gene059276 | MS.gene84001 | 0.812795 | 3.54E-51 | -1.69E-46 |
| MS.gene059458 | MS.gene84001 | 0.801295 | 9.63E-49 | -1.69E-46 |
| MS.gene059460 | MS.gene84001 | 0.803747 | 3.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCGGTTTCTTTCAATTT+TGG | 0.151259 | 1.3:-32036352 | MS.gene84001:CDS |
| ACTCCATTGTTTGTCATCTA+TGG | 0.244370 | 1.3:-32036455 | MS.gene84001:CDS |
| ATCGCCTCGGTTGCGATCTC+AGG | 0.256916 | 1.3:-32036604 | MS.gene84001:CDS |
| ATGCTGCAACCTTCAATTGC+TGG | 0.375569 | 1.3:+32036280 | None:intergenic |
| ACAGATTTGATCGGCTTAAT+CGG | 0.375588 | 1.3:+32036307 | None:intergenic |
| AAGATTTCTTTGTGCTGAAA+AGG | 0.385174 | 1.3:+32036516 | None:intergenic |
| GAAGGTTGCAGCATCAAGAT+TGG | 0.441892 | 1.3:-32036271 | MS.gene84001:CDS |
| TGGTTTCGAAGTGGCGGTAA+TGG | 0.473427 | 1.3:+32036402 | None:intergenic |
| TCTGTTATACCAGCAATTGA+AGG | 0.481754 | 1.3:-32036289 | MS.gene84001:CDS |
| TTCTTCTTCCGCTGTCGCGT+CGG | 0.512166 | 1.3:-32036574 | MS.gene84001:CDS |
| AGGAGGAGATTTCTTCGATA+CGG | 0.536796 | 1.3:+32036536 | None:intergenic |
| TACAGTTCTCTCAACTGAAG+CGG | 0.543883 | 1.3:-32036368 | MS.gene84001:CDS |
| ATTTCTTTGTGCTGAAAAGG+AGG | 0.553505 | 1.3:+32036519 | None:intergenic |
| TTCTTCGATACGGCTGCAGA+AGG | 0.558066 | 1.3:+32036546 | None:intergenic |
| GCTGGTATAACAGATTTGAT+CGG | 0.561966 | 1.3:+32036298 | None:intergenic |
| TCGCCTCGGTTGCGATCTCA+GGG | 0.565090 | 1.3:-32036603 | MS.gene84001:CDS |
| CTTCCATAGATGACAAACAA+TGG | 0.594376 | 1.3:+32036452 | None:intergenic |
| TCATCTATGGAAGAAATCAA+AGG | 0.594897 | 1.3:-32036442 | MS.gene84001:CDS |
| ATCTTGCGGTGGTTTCGAAG+TGG | 0.613519 | 1.3:+32036393 | None:intergenic |
| TCAACATGGTATCATCGCCT+CGG | 0.621028 | 1.3:-32036617 | None:intergenic |
| AGGTTTCTCCGACGCGACAG+CGG | 0.631779 | 1.3:+32036566 | None:intergenic |
| TATCCCTGAGATCGCAACCG+AGG | 0.637426 | 1.3:+32036600 | None:intergenic |
| GAGAGAACTGTAGAATCTTG+CGG | 0.642496 | 1.3:+32036379 | None:intergenic |
| TTGCGGTGGTTTCGAAGTGG+CGG | 0.652055 | 1.3:+32036396 | None:intergenic |
| AGAACTGTAGAATCTTGCGG+TGG | 0.689722 | 1.3:+32036382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAGTATGTCATTAGAAAAAA+TGG | + | chr1.3:32036392-32036411 | None:intergenic | 25.0% |
| ! | TATGTCATTAGAAAAAATGG+AGG | + | chr1.3:32036389-32036408 | None:intergenic | 25.0% |
| AAGATTTCTTTGTGCTGAAA+AGG | + | chr1.3:32036350-32036369 | None:intergenic | 30.0% | |
| TCATCTATGGAAGAAATCAA+AGG | - | chr1.3:32036421-32036440 | MS.gene84001:CDS | 30.0% | |
| ACTCCATTGTTTGTCATCTA+TGG | - | chr1.3:32036408-32036427 | MS.gene84001:CDS | 35.0% | |
| ATTTCTTTGTGCTGAAAAGG+AGG | + | chr1.3:32036347-32036366 | None:intergenic | 35.0% | |
| CTTCCATAGATGACAAACAA+TGG | + | chr1.3:32036414-32036433 | None:intergenic | 35.0% | |
| GCTGGTATAACAGATTTGAT+CGG | + | chr1.3:32036568-32036587 | None:intergenic | 35.0% | |
| TCTGTTATACCAGCAATTGA+AGG | - | chr1.3:32036574-32036593 | MS.gene84001:CDS | 35.0% | |
| ! | ACAGATTTGATCGGCTTAAT+CGG | + | chr1.3:32036559-32036578 | None:intergenic | 35.0% |
| ! | GAAGCGGTTTCTTTCAATTT+TGG | - | chr1.3:32036511-32036530 | MS.gene84001:CDS | 35.0% |
| AGGAGGAGATTTCTTCGATA+CGG | + | chr1.3:32036330-32036349 | None:intergenic | 40.0% | |
| GAGAGAACTGTAGAATCTTG+CGG | + | chr1.3:32036487-32036506 | None:intergenic | 40.0% | |
| TACAGTTCTCTCAACTGAAG+CGG | - | chr1.3:32036495-32036514 | MS.gene84001:CDS | 40.0% | |
| AGAACTGTAGAATCTTGCGG+TGG | + | chr1.3:32036484-32036503 | None:intergenic | 45.0% | |
| ATGCTGCAACCTTCAATTGC+TGG | + | chr1.3:32036586-32036605 | None:intergenic | 45.0% | |
| GAAGGTTGCAGCATCAAGAT+TGG | - | chr1.3:32036592-32036611 | MS.gene84001:CDS | 45.0% | |
| ATCTTGCGGTGGTTTCGAAG+TGG | + | chr1.3:32036473-32036492 | None:intergenic | 50.0% | |
| TGGTTTCGAAGTGGCGGTAA+TGG | + | chr1.3:32036464-32036483 | None:intergenic | 50.0% | |
| TTCTTCGATACGGCTGCAGA+AGG | + | chr1.3:32036320-32036339 | None:intergenic | 50.0% | |
| TATCCCTGAGATCGCAACCG+AGG | + | chr1.3:32036266-32036285 | None:intergenic | 55.0% | |
| TTCTTCTTCCGCTGTCGCGT+CGG | - | chr1.3:32036289-32036308 | MS.gene84001:CDS | 55.0% | |
| TTGCGGTGGTTTCGAAGTGG+CGG | + | chr1.3:32036470-32036489 | None:intergenic | 55.0% | |
| AGGTTTCTCCGACGCGACAG+CGG | + | chr1.3:32036300-32036319 | None:intergenic | 60.0% | |
| ATCGCCTCGGTTGCGATCTC+AGG | - | chr1.3:32036259-32036278 | MS.gene84001:CDS | 60.0% | |
| TCGCCTCGGTTGCGATCTCA+GGG | - | chr1.3:32036260-32036279 | MS.gene84001:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 32036251 | 32036634 | 32036251 | ID=MS.gene84001 |
| chr1.3 | mRNA | 32036251 | 32036634 | 32036251 | ID=MS.gene84001.t1;Parent=MS.gene84001 |
| chr1.3 | exon | 32036251 | 32036634 | 32036251 | ID=MS.gene84001.t1.exon1;Parent=MS.gene84001.t1 |
| chr1.3 | CDS | 32036251 | 32036634 | 32036251 | ID=cds.MS.gene84001.t1;Parent=MS.gene84001.t1 |
| Gene Sequence |
| Protein sequence |
