AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84273


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84273.t1 MTR_8g028710 82.540 315 6 1 5 270 44 358 0.0 509
MS.gene84273.t1 MTR_7g078700 56.369 314 85 2 5 269 41 351 8.88e-115 334
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84273.t1 AT2G46080 49.351 308 104 2 7 265 43 347 4.29e-93 278
MS.gene84273.t1 AT1G01550 46.758 293 98 3 7 250 43 326 3.96e-78 240
MS.gene84273.t1 AT1G01550 46.758 293 98 3 7 250 43 326 3.96e-78 240
MS.gene84273.t1 AT3G61500 34.630 257 144 7 5 258 35 270 1.51e-33 124
MS.gene84273.t1 AT3G61500 34.836 244 136 6 5 245 35 258 6.21e-33 122
MS.gene84273.t1 AT4G01360 30.435 322 142 8 11 267 43 347 6.71e-32 120

Find 65 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATTTCATCCTTGCTTTC+AGG 0.187959 8.1:+72856215 None:intergenic
GTGCTACATTTCTCAATTCT+AGG 0.226753 8.1:+72855885 None:intergenic
TTGAGCTACCCGTGAGTGTT+TGG 0.296050 8.1:-72856115 MS.gene84273:CDS
GTAGCTCAAGATCAGTTATA+AGG 0.296998 8.1:+72856129 None:intergenic
TTGAAGACTGTTGAGGAATT+AGG 0.327051 8.1:-72855495 MS.gene84273:CDS
CTCATCAAAGTCGTTTCTTC+GGG 0.357220 8.1:-72855955 MS.gene84273:CDS
CCTCATCAAAGTCGTTTCTT+CGG 0.377060 8.1:-72855956 MS.gene84273:CDS
TTGCTATCGTCTCTGAGGTT+TGG 0.380646 8.1:-72855381 MS.gene84273:CDS
CTACTTGCTGGTCTATGTTT+CGG 0.383275 8.1:+72855331 None:intergenic
AAGTGTGCTTTGCACAATTT+AGG 0.389595 8.1:-72855984 MS.gene84273:CDS
TAAATGAGGAAATTAGAGTT+AGG 0.394328 8.1:-72855641 MS.gene84273:CDS
TTCTTGCTCAACTTTGTCTT+CGG 0.412172 8.1:+72855524 None:intergenic
TTGCAAGGAGATCCATTCCT+TGG 0.419978 8.1:+72855451 None:intergenic
ATTTACACCACCTTGGATAG+TGG 0.424354 8.1:+72855548 None:intergenic
TGAGCTACCCGTGAGTGTTT+GGG 0.433640 8.1:-72856114 MS.gene84273:CDS
TGAGTGTTTGGGATGACAAA+TGG 0.445686 8.1:-72856103 MS.gene84273:CDS
GAGCTTTCTCGTCTGAATCA+AGG 0.448368 8.1:-72856020 MS.gene84273:CDS
ACCATTCTAGATGGTCTTGT+AGG 0.450952 8.1:-72855864 MS.gene84273:CDS
TGCAAGGAGATCCATTCCTT+GGG 0.451475 8.1:+72855452 None:intergenic
ATTATCAGTAAACTACTTGC+TGG 0.454398 8.1:+72855319 None:intergenic
TGCAGTACTACATGGTTTCG+AGG 0.455830 8.1:-72856271 None:intergenic
AGAAACGACTTTGATGAGGC+TGG 0.457208 8.1:+72855960 None:intergenic
AAATGTAGCACCATTCTAGA+TGG 0.460812 8.1:-72855873 MS.gene84273:CDS
ATGAAATCCTCAGCTTGTCT+TGG 0.465634 8.1:-72856202 MS.gene84273:CDS
TGCTACATTTCTCAATTCTA+GGG 0.473899 8.1:+72855886 None:intergenic
CTACATTAGCAGAGAGGCTA+AGG 0.476706 8.1:-72856247 MS.gene84273:CDS
CTTACAGAATCTTGTAAATG+AGG 0.487402 8.1:-72855655 MS.gene84273:CDS
CATGTTCTTTACTCGATGAC+TGG 0.490294 8.1:-72855932 MS.gene84273:CDS
TAGCTCAAGATCAGTTATAA+GGG 0.500951 8.1:+72856130 None:intergenic
ACTGTAAATGATCGTGTCGT+TGG 0.506521 8.1:-72855354 MS.gene84273:CDS
CTCCTTGCAAAGGGAGTGGA+TGG 0.507754 8.1:-72855438 MS.gene84273:CDS
AGGATCACTTGATCTTCCTA+AGG 0.519671 8.1:-72855844 MS.gene84273:CDS
GAAATTAGAGTTAGGTTCTC+CGG 0.520380 8.1:-72855633 MS.gene84273:CDS
CAGCTTGTCTTGGATGACTT+CGG 0.520950 8.1:-72856192 MS.gene84273:CDS
CTTCGGTATTTACACCACCT+TGG 0.523873 8.1:+72855541 None:intergenic
CACGACTAGTCAAAACTGCT+TGG 0.526184 8.1:+72855409 None:intergenic
GAGTGTTTGGGATGACAAAT+GGG 0.539560 8.1:-72856102 MS.gene84273:CDS
GAAGCTTATGCCTGAAAGCA+AGG 0.548172 8.1:-72856225 MS.gene84273:CDS
GGATCTCCTTGCAAAGGGAG+TGG 0.550124 8.1:-72855442 MS.gene84273:CDS
TTTACACCACCTTGGATAGT+GGG 0.553081 8.1:+72855549 None:intergenic
GGAATGGATCTCCTTGCAAA+GGG 0.556996 8.1:-72855447 MS.gene84273:CDS
GAAACGACTTTGATGAGGCT+GGG 0.565221 8.1:+72855961 None:intergenic
TTGTCATCCCAAACACTCAC+GGG 0.570811 8.1:+72856107 None:intergenic
GAGTTATATCCCACTATCCA+AGG 0.574910 8.1:-72855558 MS.gene84273:CDS
TCCTACAAGACCATCTAGAA+TGG 0.579676 8.1:+72855863 None:intergenic
AGGAATGGATCTCCTTGCAA+AGG 0.587585 8.1:-72855448 MS.gene84273:CDS
AACCATCCACTCCCTTTGCA+AGG 0.590151 8.1:+72855436 None:intergenic
TAGGGTTCTTAGCATTAATG+TGG 0.590455 8.1:+72855904 None:intergenic
AGAGAAGCTTTCCCAAGGAA+TGG 0.590563 8.1:-72855463 MS.gene84273:CDS
ATCACATTTGAAGACTGTTG+AGG 0.597845 8.1:-72855502 MS.gene84273:CDS
AGCTGTTGACGCTGTTGTGA+AGG 0.598706 8.1:-72855580 MS.gene84273:CDS
GTTCTTTACTCGATGACTGG+AGG 0.600909 8.1:-72855929 MS.gene84273:CDS
TTGTGCAAAGCACACTTAAG+CGG 0.616461 8.1:+72855990 None:intergenic
TTTGTCATCCCAAACACTCA+CGG 0.620658 8.1:+72856106 None:intergenic
ACACTTTGCTATCGTCTCTG+AGG 0.623338 8.1:-72855386 MS.gene84273:CDS
AAGTCATCCAAGACAAGCTG+AGG 0.623900 8.1:+72856195 None:intergenic
TTAGAGTTAGGTTCTCCGGT+GGG 0.628606 8.1:-72855629 MS.gene84273:CDS
GCAGTACTACATGGTTTCGA+GGG 0.635458 8.1:-72856270 None:intergenic
ATTAGAGTTAGGTTCTCCGG+TGG 0.639898 8.1:-72855630 MS.gene84273:CDS
CAATACAGAAAATTTCCCAC+CGG 0.653582 8.1:+72855614 None:intergenic
GCTGCAGAGAAGCTTTCCCA+AGG 0.657770 8.1:-72855468 MS.gene84273:CDS
CCGAAGAAACGACTTTGATG+AGG 0.660900 8.1:+72855956 None:intergenic
CGAGGGCTACATTAGCAGAG+AGG 0.677926 8.1:-72856253 MS.gene84273:CDS
GACTGTTGAGGAATTAGGCG+TGG 0.680712 8.1:-72855490 MS.gene84273:CDS
TTATATCCCACTATCCAAGG+TGG 0.780218 8.1:-72855555 MS.gene84273:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAATGAGGAAATTAGAGTT+AGG - chr8.1:72855942-72855961 MS.gene84273:CDS 25.0%
AAGGTTAAAAATTCTGCCAA+AGG - chr8.1:72855758-72855777 MS.gene84273:intron 30.0%
CTTACAGAATCTTGTAAATG+AGG - chr8.1:72855928-72855947 MS.gene84273:CDS 30.0%
TAAAAATTCTGCCAAAGGTA+AGG - chr8.1:72855763-72855782 MS.gene84273:intron 30.0%
TAGCTCAAGATCAGTTATAA+GGG + chr8.1:72855456-72855475 None:intergenic 30.0%
TGCTACATTTCTCAATTCTA+GGG + chr8.1:72855700-72855719 None:intergenic 30.0%
! TTGGCAGAATTTTTAACCTT+AGG + chr8.1:72855758-72855777 None:intergenic 30.0%
!! ATTTTCTGTATTGATCGAGT+TGG - chr8.1:72855979-72855998 MS.gene84273:CDS 30.0%
AAATGTAGCACCATTCTAGA+TGG - chr8.1:72855710-72855729 MS.gene84273:intron 35.0%
AAGTGTGCTTTGCACAATTT+AGG - chr8.1:72855599-72855618 MS.gene84273:CDS 35.0%
ATCACATTTGAAGACTGTTG+AGG - chr8.1:72856081-72856100 MS.gene84273:CDS 35.0%
CAATACAGAAAATTTCCCAC+CGG + chr8.1:72855972-72855991 None:intergenic 35.0%
GAAATTAGAGTTAGGTTCTC+CGG - chr8.1:72855950-72855969 MS.gene84273:CDS 35.0%
GTAGCTCAAGATCAGTTATA+AGG + chr8.1:72855457-72855476 None:intergenic 35.0%
GTGCTACATTTCTCAATTCT+AGG + chr8.1:72855701-72855720 None:intergenic 35.0%
TAGGGTTCTTAGCATTAATG+TGG + chr8.1:72855682-72855701 None:intergenic 35.0%
TTCAAAGAAGTTGCTAGACT+TGG - chr8.1:72855865-72855884 MS.gene84273:CDS 35.0%
TTCTGTAAGCCTTAAAAAGC+AGG + chr8.1:72855917-72855936 None:intergenic 35.0%
TTCTTGCTCAACTTTGTCTT+CGG + chr8.1:72856062-72856081 None:intergenic 35.0%
TTGAAGACTGTTGAGGAATT+AGG - chr8.1:72856088-72856107 MS.gene84273:CDS 35.0%
TTGCATCAAAACCTTACCTT+TGG + chr8.1:72855777-72855796 None:intergenic 35.0%
!! GTTTTGATGCAAGCTATGTA+TGG - chr8.1:72855785-72855804 MS.gene84273:intron 35.0%
ACTGTAAATGATCGTGTCGT+TGG - chr8.1:72856229-72856248 MS.gene84273:CDS 40.0%
AGGATCACTTGATCTTCCTA+AGG - chr8.1:72855739-72855758 MS.gene84273:intron 40.0%
AGGATTTCATCCTTGCTTTC+AGG + chr8.1:72855371-72855390 None:intergenic 40.0%
ATGAAATCCTCAGCTTGTCT+TGG - chr8.1:72855381-72855400 MS.gene84273:CDS 40.0%
ATTTACACCACCTTGGATAG+TGG + chr8.1:72856038-72856057 None:intergenic 40.0%
CATGTTCTTTACTCGATGAC+TGG - chr8.1:72855651-72855670 MS.gene84273:CDS 40.0%
CCTCATCAAAGTCGTTTCTT+CGG - chr8.1:72855627-72855646 MS.gene84273:CDS 40.0%
CTCATCAAAGTCGTTTCTTC+GGG - chr8.1:72855628-72855647 MS.gene84273:CDS 40.0%
GAGTTATATCCCACTATCCA+AGG - chr8.1:72856025-72856044 MS.gene84273:CDS 40.0%
TCCTACAAGACCATCTAGAA+TGG + chr8.1:72855723-72855742 None:intergenic 40.0%
TTATATCCCACTATCCAAGG+TGG - chr8.1:72856028-72856047 MS.gene84273:CDS 40.0%
TTGTGCAAAGCACACTTAAG+CGG + chr8.1:72855596-72855615 None:intergenic 40.0%
TTTACACCACCTTGGATAGT+GGG + chr8.1:72856037-72856056 None:intergenic 40.0%
TTTGTCATCCCAAACACTCA+CGG + chr8.1:72855480-72855499 None:intergenic 40.0%
! CTACTTGCTGGTCTATGTTT+CGG + chr8.1:72856255-72856274 None:intergenic 40.0%
! GAGTGTTTGGGATGACAAAT+GGG - chr8.1:72855481-72855500 MS.gene84273:CDS 40.0%
! TGAGTGTTTGGGATGACAAA+TGG - chr8.1:72855480-72855499 MS.gene84273:CDS 40.0%
!! ACCATTCTAGATGGTCTTGT+AGG - chr8.1:72855719-72855738 MS.gene84273:intron 40.0%
AAGTCATCCAAGACAAGCTG+AGG + chr8.1:72855391-72855410 None:intergenic 45.0%
AGAAACGACTTTGATGAGGC+TGG + chr8.1:72855626-72855645 None:intergenic 45.0%
AGAGAAGCTTTCCCAAGGAA+TGG - chr8.1:72856120-72856139 MS.gene84273:CDS 45.0%
AGCTATGTATGGAGTCAAGG+TGG - chr8.1:72855796-72855815 MS.gene84273:intron 45.0%
AGGAATGGATCTCCTTGCAA+AGG - chr8.1:72856135-72856154 MS.gene84273:CDS 45.0%
ATGTGCCTGACATGCATTCA+TGG - chr8.1:72855888-72855907 MS.gene84273:CDS 45.0%
ATTAGAGTTAGGTTCTCCGG+TGG - chr8.1:72855953-72855972 MS.gene84273:CDS 45.0%
CAGCTTGTCTTGGATGACTT+CGG - chr8.1:72855391-72855410 MS.gene84273:CDS 45.0%
CCGAAGAAACGACTTTGATG+AGG + chr8.1:72855630-72855649 None:intergenic 45.0%
CTACATTAGCAGAGAGGCTA+AGG - chr8.1:72855336-72855355 MS.gene84273:CDS 45.0%
CTTCGGTATTTACACCACCT+TGG + chr8.1:72856045-72856064 None:intergenic 45.0%
GAAACGACTTTGATGAGGCT+GGG + chr8.1:72855625-72855644 None:intergenic 45.0%
GAAGCTTATGCCTGAAAGCA+AGG - chr8.1:72855358-72855377 MS.gene84273:CDS 45.0%
GAGCTTTCTCGTCTGAATCA+AGG - chr8.1:72855563-72855582 MS.gene84273:CDS 45.0%
GCAAGCTATGTATGGAGTCA+AGG - chr8.1:72855793-72855812 MS.gene84273:intron 45.0%
GGAATGGATCTCCTTGCAAA+GGG - chr8.1:72856136-72856155 MS.gene84273:CDS 45.0%
GTTCTTTACTCGATGACTGG+AGG - chr8.1:72855654-72855673 MS.gene84273:CDS 45.0%
TGCAAGGAGATCCATTCCTT+GGG + chr8.1:72856134-72856153 None:intergenic 45.0%
TGTGCCTGACATGCATTCAT+GGG - chr8.1:72855889-72855908 MS.gene84273:CDS 45.0%
TTAGAGTTAGGTTCTCCGGT+GGG - chr8.1:72855954-72855973 MS.gene84273:CDS 45.0%
TTGCAAGGAGATCCATTCCT+TGG + chr8.1:72856135-72856154 None:intergenic 45.0%
TTGTCATCCCAAACACTCAC+GGG + chr8.1:72855479-72855498 None:intergenic 45.0%
! ACACTTTGCTATCGTCTCTG+AGG - chr8.1:72856197-72856216 MS.gene84273:CDS 45.0%
! CACGACTAGTCAAAACTGCT+TGG + chr8.1:72856177-72856196 None:intergenic 45.0%
! GTGTTTTCTGCAGCATTCTC+TGG - chr8.1:72855839-72855858 MS.gene84273:CDS 45.0%
!! TTGCTATCGTCTCTGAGGTT+TGG - chr8.1:72856202-72856221 MS.gene84273:CDS 45.0%
AACCATCCACTCCCTTTGCA+AGG + chr8.1:72856150-72856169 None:intergenic 50.0%
AGCTGTTGACGCTGTTGTGA+AGG - chr8.1:72856003-72856022 MS.gene84273:CDS 50.0%
GACTGTTGAGGAATTAGGCG+TGG - chr8.1:72856093-72856112 MS.gene84273:CDS 50.0%
TGAGCTACCCGTGAGTGTTT+GGG - chr8.1:72855469-72855488 MS.gene84273:CDS 50.0%
TTGAGCTACCCGTGAGTGTT+TGG - chr8.1:72855468-72855487 MS.gene84273:CDS 50.0%
!!! TCATGGGCGCCTGCTTTTTA+AGG - chr8.1:72855905-72855924 MS.gene84273:CDS 50.0%
CGAGGGCTACATTAGCAGAG+AGG - chr8.1:72855330-72855349 MS.gene84273:CDS 55.0%
CTCCTTGCAAAGGGAGTGGA+TGG - chr8.1:72856145-72856164 MS.gene84273:CDS 55.0%
GCTGCAGAGAAGCTTTCCCA+AGG - chr8.1:72856115-72856134 MS.gene84273:CDS 55.0%
GGATCTCCTTGCAAAGGGAG+TGG - chr8.1:72856141-72856160 MS.gene84273:CDS 55.0%
GGCGCCCATGAATGCATGTC+AGG + chr8.1:72855896-72855915 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 72855323 72856282 72855323 ID=MS.gene84273
chr8.1 mRNA 72855323 72856282 72855323 ID=MS.gene84273.t1;Parent=MS.gene84273
chr8.1 exon 72855826 72856282 72855826 ID=MS.gene84273.t1.exon1;Parent=MS.gene84273.t1
chr8.1 CDS 72855826 72856282 72855826 ID=cds.MS.gene84273.t1;Parent=MS.gene84273.t1
chr8.1 exon 72855323 72855678 72855323 ID=MS.gene84273.t1.exon2;Parent=MS.gene84273.t1
chr8.1 CDS 72855323 72855678 72855323 ID=cds.MS.gene84273.t1;Parent=MS.gene84273.t1
Gene Sequence

>MS.gene84273

ATGGTTTCGAGGGCTACATTAGCAGAGAGGCTAAGGAAGCTTATGCCTGAAAGCAAGGATGAAATCCTCAGCTTGTCTTGGATGACTTCGGCTATGAAGTCACTTTGTGAAACTCACAATGACATACGAACCCTTATAACTGATCTTGAGCTACCCGTGAGTGTTTGGGATGACAAATGGGTTGATGTCTACTTTGACATAAGCACAAAGTTGCTAGACATTTGCAACATATTTAGTTCTGAGCTTTCTCGTCTGAATCAAGGCAATCTTCCGCTTAAGTGTGCTTTGCACAATTTAGGCCCAGCCTCATCAAAGTCGTTTCTTCGGGCATGTTCTTTACTCGATGACTGGAGGCGCCACATTAATGCTAAGAACCCTAGAATTGAGAAATGTAGCACCATTCTAGATGGTCTTGTAGGATCACTTGATCTTCCTAAGGTTAAAAATTCTGCCAAAGGTAAGGTTTTGATGCAAGCTATGTATGGAGTCAAGGTGGAGACTGTATTTGTTTGCAGTGTGTTTTCTGCAGCATTCTCTGGCTCTTCAAAGAAGTTGCTAGACTTGGATGTGCCTGACATGCATTCATGGGCGCCTGCTTTTTAAGGCTTACAGAATCTTGTAAATGAGGAAATTAGAGTTAGGTTCTCCGGTGGGAAATTTTCTGTATTGATCGAGTTGGAAGCTGTTGACGCTGTTGTGAAGGAGTTATATCCCACTATCCAAGGTGGTGTAAATACCGAAGACAAAGTTGAGCAAGAATCACATTTGAAGACTGTTGAGGAATTAGGCGTGGCTGCAGAGAAGCTTTCCCAAGGAATGGATCTCCTTGCAAAGGGAGTGGATGGTTTCTTCCAAGCAGTTTTGACTAGTCGTGACACTTTGCTATCGTCTCTGAGGTTTGGCAAAACTGTAAATGATCGTGTCGTTGGCCGAAACATAGACCAGCAAGTAGTTTACTGA

Protein sequence

>MS.gene84273.t1

MVSRATLAERLRKLMPESKDEILSLSWMTSAMKSLCETHNDIRTLITDLELPVSVWDDKWVDVYFDISTKLLDICNIFSSELSRLNQGNLPLKCALHNLGPASSKSFLRACSLLDDWRRHINAKNPRIEKCSTILDGLVGSLDLPKVKNSAKGLQNLVNEEIRVRFSGGKFSVLIELEAVDAVVKELYPTIQGGVNTEDKVEQESHLKTVEELGVAAEKLSQGMDLLAKGVDGFFQAVLTSRDTLLSSLRFGKTVNDRVVGRNIDQQVVY