AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50248

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50248.t1 MTR_5g073210 96.964 560 16 1 1 559 1 560 0.0 1122
MS.gene50248.t1 MTR_5g083360 60.141 567 218 3 1 559 1 567 0.0 734
MS.gene50248.t1 MTR_3g071890 58.052 534 216 4 3 534 12 539 0.0 657
MS.gene50248.t1 MTR_5g020620 52.669 562 252 6 3 559 9 561 0.0 602
MS.gene50248.t1 MTR_5g020600 51.858 565 253 7 3 559 13 566 0.0 582
MS.gene50248.t1 MTR_2g008330 48.239 568 281 7 2 559 15 579 0.0 580
MS.gene50248.t1 MTR_7g060460 51.332 563 248 8 16 559 26 581 0.0 569
MS.gene50248.t1 MTR_7g062310 50.909 550 251 6 25 559 48 593 0.0 566
MS.gene50248.t1 MTR_7g062250 51.670 569 254 10 7 559 15 578 0.0 563
MS.gene50248.t1 MTR_7g458880 50.086 579 260 10 1 559 11 580 0.0 561
MS.gene50248.t1 MTR_5g069680 49.117 566 268 8 4 559 3 558 0.0 560
MS.gene50248.t1 MTR_7g058690 50.000 576 259 10 4 559 14 580 0.0 558
MS.gene50248.t1 MTR_4g015120 50.000 558 265 6 8 559 8 557 0.0 558
MS.gene50248.t1 MTR_3g462760 50.730 548 254 7 17 559 20 556 0.0 549
MS.gene50248.t1 MTR_3g101635 49.628 538 263 5 11 542 14 549 0.0 547
MS.gene50248.t1 MTR_3g101640 49.541 545 266 6 22 559 29 571 0.0 545
MS.gene50248.t1 MTR_4g064530 50.179 560 258 12 1 547 22 573 0.0 533
MS.gene50248.t1 MTR_7g065980 45.581 577 290 10 1 559 1 571 0.0 530
MS.gene50248.t1 MTR_7g065970 49.472 568 271 8 4 559 7 570 0.0 530
MS.gene50248.t1 MTR_5g081810 48.889 540 257 10 26 559 3 529 0.0 528
MS.gene50248.t1 MTR_4g019225 48.263 547 268 6 26 559 28 572 0.0 526
MS.gene50248.t1 MTR_8g027375 43.784 555 301 7 12 559 21 571 5.20e-174 504
MS.gene50248.t1 MTR_4g064530 49.540 543 252 13 1 530 22 555 6.86e-172 498
MS.gene50248.t1 MTR_8g091390 31.162 568 331 18 16 550 24 564 8.06e-68 230
MS.gene50248.t1 MTR_3g078700 31.373 561 301 21 4 534 9 515 1.37e-65 223
MS.gene50248.t1 MTR_5g081790 44.203 276 136 9 248 517 157 420 1.59e-63 214
MS.gene50248.t1 MTR_5g081790 45.752 153 76 1 18 163 18 170 1.22e-42 158
MS.gene50248.t1 MTR_3g078740 30.268 598 336 22 4 549 9 577 2.35e-62 216
MS.gene50248.t1 MTR_3g078730 29.840 563 334 19 4 549 9 527 8.69e-57 199
MS.gene50248.t1 MTR_3g008580 27.536 552 359 16 5 541 8 533 1.80e-46 172
MS.gene50248.t1 MTR_4g101650 27.241 569 376 17 4 559 6 549 6.92e-45 167
MS.gene50248.t1 MTR_1g090707 28.281 541 327 19 38 559 38 536 2.09e-44 166
MS.gene50248.t1 MTR_4g075690 28.272 573 340 21 8 559 10 532 1.14e-43 163
MS.gene50248.t1 MTR_3g083400 26.560 561 340 16 4 541 6 517 3.30e-43 162
MS.gene50248.t1 MTR_3g083430 27.469 557 340 19 4 541 6 517 4.18e-43 162
MS.gene50248.t1 MTR_3g083440 27.469 557 340 19 4 541 6 517 7.35e-43 161
MS.gene50248.t1 MTR_3g083390 27.469 557 340 19 4 541 6 517 8.11e-43 161
MS.gene50248.t1 MTR_4g049390 27.944 569 350 18 6 559 7 530 8.70e-43 160
MS.gene50248.t1 MTR_3g115050 28.571 574 350 20 1 559 5 533 5.54e-42 159
MS.gene50248.t1 MTR_4g083190 27.949 551 320 19 2 525 3 503 6.02e-42 159
MS.gene50248.t1 MTR_3g083450 27.392 533 330 18 25 541 27 518 9.00e-42 158
MS.gene50248.t1 MTR_4g083210 26.852 540 325 20 8 525 6 497 4.38e-41 156
MS.gene50248.t1 MTR_4g048350 29.222 527 316 19 45 559 48 529 1.17e-40 155
MS.gene50248.t1 MTR_5g018270 27.063 521 322 19 17 525 22 496 1.40e-39 152
MS.gene50248.t1 MTR_3g083410 26.857 525 313 15 40 541 5 481 2.07e-39 151
MS.gene50248.t1 MTR_1g026410 26.527 573 357 15 4 559 7 532 2.43e-39 151
MS.gene50248.t1 MTR_8g479380 26.945 527 337 16 8 525 10 497 8.71e-39 149
MS.gene50248.t1 MTR_8g479390 26.223 511 334 15 21 525 26 499 5.64e-38 147
MS.gene50248.t1 MTR_2g088080 29.081 533 321 18 39 559 46 533 7.51e-38 147
MS.gene50248.t1 MTR_8g073840 40.513 195 76 4 17 209 23 179 3.51e-37 139
MS.gene50248.t1 MTR_4g075690 29.158 487 275 21 8 473 10 447 1.22e-35 139
MS.gene50248.t1 MTR_7g051440 27.539 512 332 18 25 524 30 514 2.10e-35 140
MS.gene50248.t1 MTR_4g132320 27.562 566 354 20 7 559 12 534 9.53e-32 129
MS.gene50248.t1 MTR_2g088080 28.601 486 301 17 81 559 6 452 7.67e-29 119
MS.gene50248.t1 MTR_7g058670 59.259 81 32 1 81 160 13 93 3.47e-27 105
MS.gene50248.t1 MTR_3g079660 37.363 91 52 2 469 559 5 90 9.20e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50248.t1 AT2G30210 70.160 563 159 2 5 559 9 570 0.0 830
MS.gene50248.t1 AT5G07130 65.439 570 185 5 1 559 1 569 0.0 764
MS.gene50248.t1 AT5G05390 60.428 561 215 5 3 559 8 565 0.0 717
MS.gene50248.t1 AT2G40370 58.333 576 222 4 2 559 5 580 0.0 714
MS.gene50248.t1 AT5G07130 68.041 485 143 5 86 559 1 484 0.0 668
MS.gene50248.t1 AT5G07130 68.041 485 143 5 86 559 1 484 0.0 668
MS.gene50248.t1 AT2G29130 52.356 573 252 9 4 559 5 573 0.0 579
MS.gene50248.t1 AT5G09360 49.378 563 273 7 1 559 15 569 0.0 574
MS.gene50248.t1 AT5G03260 50.980 561 259 7 4 559 8 557 0.0 567
MS.gene50248.t1 AT5G60020 51.786 560 237 10 25 559 26 577 0.0 566
MS.gene50248.t1 AT5G60020 52.545 550 228 10 35 559 4 545 0.0 563
MS.gene50248.t1 AT2G38080 49.735 567 268 8 1 559 1 558 0.0 556
MS.gene50248.t1 AT5G58910 48.940 566 270 6 4 559 11 567 0.0 545
MS.gene50248.t1 AT5G58910 48.940 566 269 7 4 559 11 566 0.0 544
MS.gene50248.t1 AT5G01190 49.821 560 268 6 5 559 7 558 0.0 539
MS.gene50248.t1 AT2G46570 45.907 562 293 7 5 559 12 569 0.0 527
MS.gene50248.t1 AT5G58910 49.719 533 243 6 37 559 6 523 0.0 524
MS.gene50248.t1 AT5G01190 51.336 524 243 5 41 559 28 544 0.0 524
MS.gene50248.t1 AT3G09220 49.275 552 258 9 4 542 8 550 0.0 521
MS.gene50248.t1 AT1G18140 47.257 565 270 9 17 559 23 581 4.52e-178 515
MS.gene50248.t1 AT5G60020 51.737 518 217 10 25 517 26 535 2.77e-177 512
MS.gene50248.t1 AT5G01040 45.536 560 286 7 11 559 15 566 9.97e-173 501
MS.gene50248.t1 AT5G01050 45.018 562 288 7 11 559 15 568 8.94e-168 489
MS.gene50248.t1 AT5G48100 45.149 536 280 9 24 549 22 553 9.72e-168 488
MS.gene50248.t1 AT2G46570 46.186 485 251 6 81 559 4 484 2.47e-157 458
MS.gene50248.t1 AT3G09220 49.455 459 212 8 97 542 2 453 2.49e-148 435
MS.gene50248.t1 AT5G21100 31.332 533 304 17 39 538 39 542 7.47e-69 233
MS.gene50248.t1 AT4G39830 31.351 555 302 21 39 549 51 570 1.82e-68 232
MS.gene50248.t1 AT5G21105 30.823 571 323 22 5 537 22 558 1.78e-64 221
MS.gene50248.t1 AT5G21105 30.309 551 312 22 5 517 22 538 5.81e-55 194
MS.gene50248.t1 AT4G39830 30.315 508 276 20 85 549 1 473 8.56e-51 182
MS.gene50248.t1 AT4G39830 30.357 504 273 20 89 549 7 475 3.94e-50 180
MS.gene50248.t1 AT1G75790 28.224 535 328 15 1 519 1 495 4.84e-49 178
MS.gene50248.t1 AT4G25240 29.247 571 357 18 4 559 12 550 2.00e-47 174
MS.gene50248.t1 AT4G25240 29.247 571 357 18 4 559 12 550 2.00e-47 174
MS.gene50248.t1 AT4G39830 29.622 503 265 19 89 549 7 462 2.27e-46 169
MS.gene50248.t1 AT4G12420 27.850 535 350 14 5 527 6 516 3.88e-46 171
MS.gene50248.t1 AT4G12420 27.850 535 350 14 5 527 6 516 3.88e-46 171
MS.gene50248.t1 AT5G66920 27.748 555 327 17 25 559 38 538 1.58e-44 166
MS.gene50248.t1 AT1G21850 28.860 544 302 18 8 525 12 496 5.76e-44 164
MS.gene50248.t1 AT5G51480 26.916 561 369 17 14 559 16 550 6.79e-43 162
MS.gene50248.t1 AT5G48450 27.191 559 354 19 8 556 13 528 8.71e-43 162
MS.gene50248.t1 AT5G48450 27.128 564 348 20 8 556 13 528 1.93e-42 161
MS.gene50248.t1 AT1G21860 26.679 536 325 15 8 525 11 496 5.34e-41 155
MS.gene50248.t1 AT4G22010 27.076 554 365 14 11 559 11 530 1.29e-40 155
MS.gene50248.t1 AT1G76160 27.599 558 359 17 8 559 12 530 3.28e-40 154
MS.gene50248.t1 AT1G41830 26.195 565 366 16 3 559 10 531 5.92e-39 150
MS.gene50248.t1 AT4G28090 25.788 539 332 15 6 524 9 499 2.48e-38 148
MS.gene50248.t1 AT1G55570 25.783 543 327 16 4 519 7 500 1.51e-36 143
MS.gene50248.t1 AT2G23630 29.412 527 321 19 42 559 48 532 1.35e-35 140
MS.gene50248.t1 AT2G23630 29.412 527 321 19 42 559 53 537 1.48e-35 140
MS.gene50248.t1 AT4G38420 26.208 538 336 18 6 524 9 504 6.49e-35 139
MS.gene50248.t1 AT4G37160 27.224 562 368 16 2 559 10 534 1.90e-33 134
MS.gene50248.t1 AT5G21105 30.205 341 202 11 209 537 51 367 1.11e-32 130
MS.gene50248.t1 AT3G13390 25.670 522 310 17 25 519 29 499 2.26e-32 131
MS.gene50248.t1 AT1G55560 25.468 534 338 19 4 519 5 496 1.24e-31 129
MS.gene50248.t1 AT3G13400 25.421 535 337 20 4 519 6 497 2.12e-30 125
MS.gene50248.t1 AT3G13400 25.421 535 337 20 4 519 6 497 2.12e-30 125
MS.gene50248.t1 AT4G38420 25.977 512 318 18 6 498 9 478 7.07e-30 123
MS.gene50248.t1 AT4G38420 31.408 277 177 7 6 278 9 276 2.28e-29 121
MS.gene50248.t1 AT4G37160 28.990 307 197 6 2 305 10 298 1.73e-25 109

Find 146 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCGAAACACTTTAATTT+TGG 0.112254 5.2:+61324215 None:intergenic
CCTTGACACCTTGGACTATT+AGG 0.188458 5.2:+61324138 None:intergenic
TTTATAGTTGGTTCAGGTTT+TGG 0.199584 5.2:-61323817 MS.gene50248:CDS
GCCACCATAGTGTTCCTTCT+TGG 0.231177 5.2:+61325555 None:intergenic
CGAAGGATACTCACCGTAAA+TGG 0.256488 5.2:-61325905 MS.gene50248:CDS
GTCTTGAAGGTGGTCTATTT+TGG 0.257321 5.2:+61324313 None:intergenic
ATTGTTGTGAATCAGGAATT+TGG 0.267922 5.2:-61323391 MS.gene50248:intron
AAGTTGTTCAAGTTGAAATA+TGG 0.300472 5.2:-61323922 MS.gene50248:CDS
AGAGAATTTCCCGTTCTTCT+TGG 0.306078 5.2:-61325440 MS.gene50248:intron
AATGGTAAAGCCGGCAAAAT+AGG 0.318344 5.2:+61324291 None:intergenic
GCTTACTACAATGGTGTTCC+TGG 0.329058 5.2:-61324039 MS.gene50248:CDS
CATTTCTTTATAGTTGGTTC+AGG 0.332092 5.2:-61323823 MS.gene50248:CDS
CTCCATAGACAGTGGCTCTT+AGG 0.334059 5.2:+61325516 None:intergenic
AACGTAGCAGGATCAGTTCT+TGG 0.335909 5.2:+61323784 None:intergenic
AAGGTCCAGCATTTGTTACT+TGG 0.341260 5.2:+61325831 None:intergenic
ACATAGATTCACATCTCAAT+TGG 0.345519 5.2:-61323355 MS.gene50248:CDS
GCCAGGAAACTGTCCATTTA+CGG 0.347312 5.2:+61325892 None:intergenic
CCGTCCCTCGCTTCGATCGT+TGG 0.351478 5.2:+61325869 None:intergenic
TTCCTAAGAGCCACTGTCTA+TGG 0.354270 5.2:-61325518 MS.gene50248:CDS
GGGTTTGGCAACAGCACTTT+TGG 0.375326 5.2:-61323333 MS.gene50248:CDS
TTCACATCTCAATTGGGGTT+TGG 0.376065 5.2:-61323348 MS.gene50248:CDS
GGGTTCCATTTCTTTATAGT+TGG 0.378042 5.2:-61323829 MS.gene50248:CDS
CATAGATTCACATCTCAATT+GGG 0.380545 5.2:-61323354 MS.gene50248:CDS
CCATTTGGTCCTTGACACCT+TGG 0.385637 5.2:+61324129 None:intergenic
GCTGCAAATCTTGTTCCATT+TGG 0.386553 5.2:+61324114 None:intergenic
TGCATTAAGGAAGTTGTTCT+AGG 0.389050 5.2:+61324063 None:intergenic
ATGCATTATACCTGGATTAT+CGG 0.389442 5.2:+61323687 None:intergenic
ACCGTAAATGGACAGTTTCC+TGG 0.396378 5.2:-61325893 MS.gene50248:CDS
GTTGTCAAATGCAGCATTCA+TGG 0.401840 5.2:+61324362 None:intergenic
GAATGATTTCAAACCTTGGC+TGG 0.402430 5.2:+61324864 None:intergenic
ATGGGTCGCCATTCGTTTCA+AGG 0.412325 5.2:-61323710 MS.gene50248:CDS
CCACCTGTGAGGAATACTAT+TGG 0.412353 5.2:-61323748 MS.gene50248:CDS
ATCTCCTGGTTGACCGTTCA+TGG 0.413530 5.2:+61324900 None:intergenic
CGTCCCTCGCTTCGATCGTT+GGG 0.413929 5.2:+61325870 None:intergenic
TGTGCAACAGGCATGGGCTT+AGG 0.415052 5.2:-61325673 MS.gene50248:intron
GCATTCACATCAAGGATTAG+AGG 0.421253 5.2:-61324243 MS.gene50248:CDS
TTATTGTTCACTGTAGGGTT+AGG 0.421636 5.2:-61324180 MS.gene50248:CDS
CTGAACCAACTATAAAGAAA+TGG 0.426203 5.2:+61323824 None:intergenic
TAACTTCCTCCTGGTTGGAT+TGG 0.434515 5.2:+61325602 None:intergenic
ATGCAGCATTCATGGCGGTT+TGG 0.436299 5.2:+61324370 None:intergenic
GAGGTATCACTGACTGTAAT+GGG 0.438774 5.2:+61323294 None:intergenic
TTGAACTGTATAGGAGGAAC+AGG 0.439285 5.2:+61323994 None:intergenic
GATCACCCTATGCATGTTCA+TGG 0.442341 5.2:-61323850 MS.gene50248:CDS
GAAATGTTCCTAGGGGTTTG+TGG 0.445135 5.2:-61323963 MS.gene50248:CDS
CTATGGTGGCATGCTCATAC+TGG 0.446683 5.2:-61325542 MS.gene50248:CDS
TCTCTAGTTGGCTTAGAGAA+AGG 0.447045 5.2:+61325458 None:intergenic
TGAAGATTACCAAGAAGAAC+GGG 0.448241 5.2:+61325431 None:intergenic
AGAACGGGAAATTCTCTAGT+TGG 0.448541 5.2:+61325446 None:intergenic
CTCGCTTCGATCGTTGGGCC+AGG 0.448652 5.2:+61325875 None:intergenic
ATTACACAGGAAATGTTCCT+AGG 0.452764 5.2:-61323972 MS.gene50248:CDS
CGTTTCAAGGCCGATAATCC+AGG 0.464778 5.2:-61323697 MS.gene50248:intron
CCTGTGTAATTGAACTGTAT+AGG 0.466472 5.2:+61323985 None:intergenic
CTGCAAAACAATTTGAACAT+TGG 0.467774 5.2:+61323897 None:intergenic
GTTTGTCTTAAGAGAGCCAT+TGG 0.471023 5.2:+61324964 None:intergenic
CCAAGGTGTCAAGGACCAAA+TGG 0.477385 5.2:-61324129 MS.gene50248:CDS
GCCGGCAAAATAGGTCTTGA+AGG 0.477836 5.2:+61324300 None:intergenic
GTTGGCTTAGAGAAAGGATA+AGG 0.479975 5.2:+61325464 None:intergenic
GTGTATGCAACAGATACATT+TGG 0.482818 5.2:+61324922 None:intergenic
GTGAGTTTATTGTTCACTGT+AGG 0.484623 5.2:-61324186 MS.gene50248:CDS
ATTGTAGTAAGCTTGCATTA+AGG 0.490056 5.2:+61324050 None:intergenic
TCGACAAGGTTGAACGTAGC+AGG 0.491890 5.2:+61323772 None:intergenic
TGAGTTTATTGTTCACTGTA+GGG 0.502703 5.2:-61324185 MS.gene50248:CDS
GGAGGTATCACTGACTGTAA+TGG 0.504892 5.2:+61323293 None:intergenic
TTGGAACACCTCCTGGTGGA+TGG 0.505700 5.2:-61323729 MS.gene50248:CDS
AATACTATTGGAACACCTCC+TGG 0.507986 5.2:-61323736 MS.gene50248:CDS
CTCTATAGATGTTCCAGCCA+AGG 0.510832 5.2:-61324877 MS.gene50248:intron
TGGAACACCTCCTGGTGGAT+GGG 0.516621 5.2:-61323728 MS.gene50248:CDS
AAAACCAAGAAGGAACACTA+TGG 0.519751 5.2:-61325559 MS.gene50248:CDS
CAGTGGATGGAGATGTTGTA+AGG 0.523772 5.2:+61325812 None:intergenic
ATGTGTAACTTCCTCCTGGT+TGG 0.527858 5.2:+61325597 None:intergenic
GTCACAGCCGATCAACCACC+TGG 0.528760 5.2:-61324420 MS.gene50248:CDS
TTGAAGATTACCAAGAAGAA+CGG 0.530236 5.2:+61325430 None:intergenic
GCTGCCATGTAGTATCGGCC+AGG 0.530922 5.2:+61324402 None:intergenic
CTGCAACTGCATTCACATCA+AGG 0.532127 5.2:-61324251 MS.gene50248:CDS
GTGATCCAAGTAACAAATGC+TGG 0.533819 5.2:-61325836 MS.gene50248:CDS
GAACATCTATAGAGATCTCC+TGG 0.536997 5.2:+61324886 None:intergenic
TGAGGTAGATCTGGTGGTGG+AGG 0.538755 5.2:+61323275 None:intergenic
GGACATTGTGTCACATAACT+AGG 0.539105 5.2:+61325623 None:intergenic
TGGAACCCATGAACATGCAT+AGG 0.539781 5.2:+61323844 None:intergenic
AACACTAGAGTCCTCATGAT+AGG 0.541605 5.2:-61324468 MS.gene50248:CDS
CGGTATGTGTAACTTCCTCC+TGG 0.542556 5.2:+61325593 None:intergenic
AGAGTCCTCATGATAGGTCC+AGG 0.542967 5.2:-61324462 MS.gene50248:CDS
TTTACAATTCAAAACCAAGA+AGG 0.543934 5.2:-61325569 MS.gene50248:CDS
TGTTTCATTTGCTGCAGCAG+AGG 0.548223 5.2:-61326074 MS.gene50248:CDS
ATATTTCAACTTGAACAACT+TGG 0.548915 5.2:+61323924 None:intergenic
TATCATGAGGACTCTAGTGT+TGG 0.549209 5.2:+61324470 None:intergenic
CGATGATAGTTACCAGTGGA+TGG 0.549490 5.2:+61325799 None:intergenic
GTATCGGCCAGGTGGTTGAT+CGG 0.551980 5.2:+61324413 None:intergenic
TTGAGAAATCCATGGGCAGA+TGG 0.554935 5.2:-61325647 MS.gene50248:CDS
CTAGGTGTCCACAAACCCCT+AGG 0.555841 5.2:+61323955 None:intergenic
CGCGTGCTGCCATGTAGTAT+CGG 0.558373 5.2:+61324397 None:intergenic
GACATATACTTGAAAACGTA+CGG 0.558442 5.2:+61324608 None:intergenic
TTACACAGGAAATGTTCCTA+GGG 0.560206 5.2:-61323971 MS.gene50248:CDS
ACCACCTGGCCGATACTACA+TGG 0.560583 5.2:-61324406 MS.gene50248:CDS
CCTAATAGTCCAAGGTGTCA+AGG 0.569314 5.2:-61324138 MS.gene50248:CDS
AGGACATTGATTGTTTGACC+TGG 0.570579 5.2:+61324444 None:intergenic
TAGCATTGAGGTAGATCTGG+TGG 0.573248 5.2:+61323269 None:intergenic
CGAATGGCGACCCATCCACC+AGG 0.580203 5.2:+61323718 None:intergenic
ATGGTTTGATAGAGATCCAA+TGG 0.580804 5.2:-61324980 MS.gene50248:CDS
GGTTTGTGGACACCTAGAAA+AGG 0.582031 5.2:-61323949 MS.gene50248:CDS
TACTAGTATAGTAACAATTG+AGG 0.584246 5.2:-61323872 MS.gene50248:CDS
TTAAGACAAACACAGTTCAC+AGG 0.584771 5.2:-61324952 MS.gene50248:CDS
TACACCATGAACGGTCAACC+AGG 0.586391 5.2:-61324904 MS.gene50248:CDS
ATGAGGACTCTAGTGTTGGA+AGG 0.587715 5.2:+61324474 None:intergenic
TATGCAACAGATACATTTGG+AGG 0.588595 5.2:+61324925 None:intergenic
TTAATGCAAGCTTACTACAA+TGG 0.590912 5.2:-61324048 MS.gene50248:CDS
TTCAAACTGCAACAGTGAAG+AGG 0.591115 5.2:-61325940 MS.gene50248:CDS
ATCACCCTATGCATGTTCAT+GGG 0.594471 5.2:-61323849 MS.gene50248:CDS
GTGGTTGATCGGCTGTGACA+AGG 0.595575 5.2:+61324424 None:intergenic
ATAGATTCACATCTCAATTG+GGG 0.596176 5.2:-61323353 MS.gene50248:CDS
CCAACGATCGAAGCGAGGGA+CGG 0.596308 5.2:-61325869 MS.gene50248:CDS
CAGAATGACTGTTGTTGCTA+CGG 0.601727 5.2:-61324514 MS.gene50248:CDS
GCACTAATCCTAATAGTCCA+AGG 0.603359 5.2:-61324146 MS.gene50248:CDS
TGGCCCAACGATCGAAGCGA+GGG 0.604197 5.2:-61325873 MS.gene50248:CDS
AAACACTTACAACAAACTGA+TGG 0.608462 5.2:+61326044 None:intergenic
CATTGAGGTAGATCTGGTGG+TGG 0.610540 5.2:+61323272 None:intergenic
TTAGGATGTTGAGAAATCCA+TGG 0.611446 5.2:-61325655 MS.gene50248:CDS
GTGTAATTGAACTGTATAGG+AGG 0.616090 5.2:+61323988 None:intergenic
CTGGCCCAACGATCGAAGCG+AGG 0.616399 5.2:-61325874 MS.gene50248:CDS
GGAACCCATGAACATGCATA+GGG 0.618003 5.2:+61323845 None:intergenic
CCTATACAGTTCAATTACAC+AGG 0.618962 5.2:-61323985 MS.gene50248:CDS
TAGGATGTTGAGAAATCCAT+GGG 0.620108 5.2:-61325654 MS.gene50248:CDS
ATTATCGGCCTTGAAACGAA+TGG 0.624949 5.2:+61323702 None:intergenic
CCAATAGTATTCCTCACAGG+TGG 0.626541 5.2:+61323748 None:intergenic
TAACTAGGTCCATCTGCCCA+TGG 0.627031 5.2:+61325638 None:intergenic
CTTACAACATCTCCATCCAC+TGG 0.628162 5.2:-61325811 MS.gene50248:intron
TCCTCACAGGTGGATCGACA+AGG 0.628411 5.2:+61323758 None:intergenic
GTTCCAATAGTATTCCTCAC+AGG 0.630506 5.2:+61323745 None:intergenic
AACTGTATAGGAGGAACAGG+AGG 0.636960 5.2:+61323997 None:intergenic
ACACAATGTCCAATCCAACC+AGG 0.647093 5.2:-61325611 MS.gene50248:CDS
TCTGTTGCATACACCATGAA+CGG 0.651763 5.2:-61324913 MS.gene50248:CDS
TACACAGGAAATGTTCCTAG+GGG 0.663460 5.2:-61323970 MS.gene50248:CDS
TGATGTGAATGCAGTTGCAG+TGG 0.665699 5.2:+61324254 None:intergenic
ACCAAGAAGGAACACTATGG+TGG 0.666226 5.2:-61325556 MS.gene50248:CDS
TTTGACCTGGACCTATCATG+AGG 0.666731 5.2:+61324457 None:intergenic
TAGCAACAACAGTCATTCTG+TGG 0.668466 5.2:+61324517 None:intergenic
AATGAAAGCTCCATAGACAG+TGG 0.677360 5.2:+61325508 None:intergenic
ACTATTGGAACACCTCCTGG+TGG 0.679776 5.2:-61323733 MS.gene50248:CDS
ACCTTGTCGATCCACCTGTG+AGG 0.686020 5.2:-61323759 MS.gene50248:CDS
GTCAAATGCAGCATTCATGG+CGG 0.689483 5.2:+61324365 None:intergenic
GGCAAAATAGGTCTTGAAGG+TGG 0.702577 5.2:+61324303 None:intergenic
ATGGCGACCCATCCACCAGG+AGG 0.719284 5.2:+61323721 None:intergenic
AGAGGCTGTGCAAAACACGA+AGG 0.719687 5.2:-61325922 MS.gene50248:CDS
GCCATGTAGTATCGGCCAGG+TGG 0.720037 5.2:+61324405 None:intergenic
AGGAACAGGAGGAAAATCTG+TGG 0.731627 5.2:+61324008 None:intergenic
CAATGTCCAATCCAACCAGG+AGG 0.746956 5.2:-61325608 MS.gene50248:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTCTGTAATATAA+AGG + chr5.2:61324611-61324630 None:intergenic 10.0%
!! TTCAAATTTAAATTCAGATT+TGG - chr5.2:61324117-61324136 MS.gene50248:CDS 15.0%
!! TTCATTATTTATCCAAAAAT+GGG - chr5.2:61323895-61323914 MS.gene50248:CDS 15.0%
!! TTTCATTATTTATCCAAAAA+TGG - chr5.2:61323894-61323913 MS.gene50248:CDS 15.0%
!!! ATTTGTTTGAGTATTTTTTT+AGG + chr5.2:61324261-61324280 None:intergenic 15.0%
!!! TAAATACCAAGTTTAAAATA+TGG - chr5.2:61324155-61324174 MS.gene50248:CDS 15.0%
!!! TTTGTTTGAGTATTTTTTTA+GGG + chr5.2:61324260-61324279 None:intergenic 15.0%
!! AAAAACATATTCTGTCAATA+TGG + chr5.2:61324371-61324390 None:intergenic 20.0%
!! AAACTCAATCCTTTATATTA+CGG - chr5.2:61324671-61324690 MS.gene50248:intron 20.0%
!! TATAAAAACAATTACAATGC+AGG - chr5.2:61324753-61324772 MS.gene50248:intron 20.0%
!! TGAATTCAAAAATCAACATT+TGG + chr5.2:61325976-61325995 None:intergenic 20.0%
!!! AATATGTTTTTATGATGCAT+AGG - chr5.2:61324380-61324399 MS.gene50248:CDS 20.0%
!!! AATTCAAATCCTATTTTGAA+CGG + chr5.2:61324026-61324045 None:intergenic 20.0%
!!! ATATGTTTTTATGATGCATA+GGG - chr5.2:61324381-61324400 MS.gene50248:CDS 20.0%
!!! ATGTGAATGTTTTTAAATCA+TGG - chr5.2:61325884-61325903 MS.gene50248:CDS 20.0%
!!! ATTTCGAAACACTTTAATTT+TGG + chr5.2:61325174-61325193 None:intergenic 20.0%
! ATAGTGAATGTTTGGATAAA+CGG + chr5.2:61323666-61323685 None:intergenic 25.0%
! ATATTTCAACTTGAACAACT+TGG + chr5.2:61325465-61325484 None:intergenic 25.0%
! TAACATAAATGCATTATACC+TGG + chr5.2:61325710-61325729 None:intergenic 25.0%
! TAACTAACTTTAATGATTGC+TGG - chr5.2:61323416-61323435 MS.gene50248:intron 25.0%
! TACTAGTATAGTAACAATTG+AGG - chr5.2:61325514-61325533 MS.gene50248:CDS 25.0%
! TAGTTAATACAGTTGCAAAT+TGG + chr5.2:61323402-61323421 None:intergenic 25.0%
! TCTCTTGCATAATTTATACT+AGG - chr5.2:61325926-61325945 MS.gene50248:CDS 25.0%
! TTAGATAAACCGTTCAAAAT+AGG - chr5.2:61324014-61324033 MS.gene50248:CDS 25.0%
! TTTACAATTCAAAACCAAGA+AGG - chr5.2:61323817-61323836 MS.gene50248:CDS 25.0%
!! AAGTATTTAAGACCATAATG+AGG + chr5.2:61323621-61323640 None:intergenic 25.0%
!! GTTGATAAAAAAGTGACTAA+AGG - chr5.2:61324730-61324749 MS.gene50248:intron 25.0%
!! TTTAGAATGATTTCAAACCT+TGG + chr5.2:61324529-61324548 None:intergenic 25.0%
!!! AAGTTGTTCAAGTTGAAATA+TGG - chr5.2:61325464-61325483 MS.gene50248:CDS 25.0%
!!! AATGGTCCATATTTTAAACT+TGG + chr5.2:61324164-61324183 None:intergenic 25.0%
!!! ACTATTTTATGCTACTTACT+TGG - chr5.2:61323274-61323293 MS.gene50248:CDS 25.0%
!!! CTTGGTTTTGAATTGTAAAT+CGG + chr5.2:61323816-61323835 None:intergenic 25.0%
!!! GAGTATTTTTTTAGGGATTT+GGG + chr5.2:61324253-61324272 None:intergenic 25.0%
!!! TATGTTTTTATGATGCATAG+GGG - chr5.2:61324382-61324401 MS.gene50248:CDS 25.0%
!!! TGAGTATTTTTTTAGGGATT+TGG + chr5.2:61324254-61324273 None:intergenic 25.0%
!!! TTTACCATTTTTTAACGACA+CGG - chr5.2:61325109-61325128 MS.gene50248:intron 25.0%
AAAACGATGTATGGAAGTAA+TGG + chr5.2:61324555-61324574 None:intergenic 30.0%
AAACACTTACAACAAACTGA+TGG + chr5.2:61323345-61323364 None:intergenic 30.0%
ACATAGATTCACATCTCAAT+TGG - chr5.2:61326031-61326050 MS.gene50248:intron 30.0%
ATAGATTCACATCTCAATTG+GGG - chr5.2:61326033-61326052 MS.gene50248:intron 30.0%
ATGCATTATACCTGGATTAT+CGG + chr5.2:61325702-61325721 None:intergenic 30.0%
ATTATGCAAGAGAGAGATAT+CGG + chr5.2:61325919-61325938 None:intergenic 30.0%
ATTGTAGTAAGCTTGCATTA+AGG + chr5.2:61325339-61325358 None:intergenic 30.0%
ATTGTTGTGAATCAGGAATT+TGG - chr5.2:61325995-61326014 MS.gene50248:intron 30.0%
CATAGATTCACATCTCAATT+GGG - chr5.2:61326032-61326051 MS.gene50248:intron 30.0%
CTGAACCAACTATAAAGAAA+TGG + chr5.2:61325565-61325584 None:intergenic 30.0%
CTGCAAAACAATTTGAACAT+TGG + chr5.2:61325492-61325511 None:intergenic 30.0%
GACATATACTTGAAAACGTA+CGG + chr5.2:61324781-61324800 None:intergenic 30.0%
TAGTGAATGTTTGGATAAAC+GGG + chr5.2:61323665-61323684 None:intergenic 30.0%
TCGTTAAAAAATGGTAAAGC+CGG + chr5.2:61325107-61325126 None:intergenic 30.0%
TGAATTCATTGTTGTGAATC+AGG - chr5.2:61325988-61326007 MS.gene50248:intron 30.0%
TGAGTTTATTGTTCACTGTA+GGG - chr5.2:61325201-61325220 MS.gene50248:intron 30.0%
TTAATGCAAGCTTACTACAA+TGG - chr5.2:61325338-61325357 MS.gene50248:intron 30.0%
TTGAAGATTACCAAGAAGAA+CGG + chr5.2:61323959-61323978 None:intergenic 30.0%
! CATTTCTTTATAGTTGGTTC+AGG - chr5.2:61325563-61325582 MS.gene50248:CDS 30.0%
! CTGCAATTTAAAACGATGTA+TGG + chr5.2:61324564-61324583 None:intergenic 30.0%
! TGTTTGTGTCAGTGTTTTAT+AGG - chr5.2:61325859-61325878 MS.gene50248:CDS 30.0%
!! ATGTTTTTAAATCATGGTCG+CGG - chr5.2:61325890-61325909 MS.gene50248:CDS 30.0%
!! CAATGTTCAAATTGTTTTGC+AGG - chr5.2:61325490-61325509 MS.gene50248:CDS 30.0%
!! TTTATAGTTGGTTCAGGTTT+TGG - chr5.2:61325569-61325588 MS.gene50248:CDS 30.0%
!!! GTACGTTTTCAAGTATATGT+CGG - chr5.2:61324780-61324799 MS.gene50248:intron 30.0%
AAAACCAAGAAGGAACACTA+TGG - chr5.2:61323827-61323846 MS.gene50248:CDS 35.0%
AAATCTGTGGTAAAAACACC+AGG + chr5.2:61325368-61325387 None:intergenic 35.0%
AGACACATAAGAAGATTGTC+AGG + chr5.2:61323692-61323711 None:intergenic 35.0%
ATGGTTTGATAGAGATCCAA+TGG - chr5.2:61324406-61324425 MS.gene50248:CDS 35.0%
ATTACACAGGAAATGTTCCT+AGG - chr5.2:61325414-61325433 MS.gene50248:intron 35.0%
CCTATACAGTTCAATTACAC+AGG - chr5.2:61325401-61325420 MS.gene50248:intron 35.0%
CCTGTGTAATTGAACTGTAT+AGG + chr5.2:61325404-61325423 None:intergenic 35.0%
GGGTTCCATTTCTTTATAGT+TGG - chr5.2:61325557-61325576 MS.gene50248:CDS 35.0%
GTGAGTTTATTGTTCACTGT+AGG - chr5.2:61325200-61325219 MS.gene50248:intron 35.0%
GTGTAATTGAACTGTATAGG+AGG + chr5.2:61325401-61325420 None:intergenic 35.0%
GTGTATGCAACAGATACATT+TGG + chr5.2:61324467-61324486 None:intergenic 35.0%
TAGGATGTTGAGAAATCCAT+GGG - chr5.2:61323732-61323751 MS.gene50248:CDS 35.0%
TATGCAACAGATACATTTGG+AGG + chr5.2:61324464-61324483 None:intergenic 35.0%
TCAGGAACATAGTGAATGTT+TGG + chr5.2:61323674-61323693 None:intergenic 35.0%
TCGATTGGATCAAAATTGGA+CGG - chr5.2:61324094-61324113 MS.gene50248:CDS 35.0%
TGAAGATTACCAAGAAGAAC+GGG + chr5.2:61323958-61323977 None:intergenic 35.0%
TGCATTAAGGAAGTTGTTCT+AGG + chr5.2:61325326-61325345 None:intergenic 35.0%
TGTCTGCAACCGTAATATAA+AGG + chr5.2:61324683-61324702 None:intergenic 35.0%
TTAAGACAAACACAGTTCAC+AGG - chr5.2:61324434-61324453 MS.gene50248:CDS 35.0%
TTACACAGGAAATGTTCCTA+GGG - chr5.2:61325415-61325434 MS.gene50248:intron 35.0%
TTAGGATGTTGAGAAATCCA+TGG - chr5.2:61323731-61323750 MS.gene50248:CDS 35.0%
TTATTGTTCACTGTAGGGTT+AGG - chr5.2:61325206-61325225 MS.gene50248:intron 35.0%
! AACAACTTGGTTCCTTTTCT+AGG + chr5.2:61325452-61325471 None:intergenic 35.0%
! ACAGCACTTTTGGTAGAAAA+TGG - chr5.2:61326063-61326082 MS.gene50248:CDS 35.0%
! CTTTTGGTAGAAAATGGAGT+TGG - chr5.2:61326069-61326088 MS.gene50248:CDS 35.0%
! TTTTATGATGCATAGGGGAA+TGG - chr5.2:61324387-61324406 MS.gene50248:CDS 35.0%
!! AATTTTGATCCAATCGACCA+CGG + chr5.2:61324091-61324110 None:intergenic 35.0%
!! ACATCGTTTTAAATTGCAGC+TGG - chr5.2:61324565-61324584 MS.gene50248:CDS 35.0%
AACACTAGAGTCCTCATGAT+AGG - chr5.2:61324918-61324937 MS.gene50248:CDS 40.0%
AATACTATTGGAACACCTCC+TGG - chr5.2:61325650-61325669 MS.gene50248:CDS 40.0%
AATGAAAGCTCCATAGACAG+TGG + chr5.2:61323881-61323900 None:intergenic 40.0%
AATGGTAAAGCCGGCAAAAT+AGG + chr5.2:61325098-61325117 None:intergenic 40.0%
ACATCAGTGGCCATTGATAA+AGG + chr5.2:61324190-61324209 None:intergenic 40.0%
AGAACGGGAAATTCTCTAGT+TGG + chr5.2:61323943-61323962 None:intergenic 40.0%
AGAGAATTTCCCGTTCTTCT+TGG - chr5.2:61323946-61323965 MS.gene50248:CDS 40.0%
ATCACCCTATGCATGTTCAT+GGG - chr5.2:61325537-61325556 MS.gene50248:CDS 40.0%
ATTATCGGCCTTGAAACGAA+TGG + chr5.2:61325687-61325706 None:intergenic 40.0%
CAGAATGACTGTTGTTGCTA+CGG - chr5.2:61324872-61324891 MS.gene50248:intron 40.0%
CTATAGAACCAATACGACAC+TGG - chr5.2:61325759-61325778 MS.gene50248:intron 40.0%
CTTATGTGTCTTTGTGCAAC+AGG - chr5.2:61323701-61323720 MS.gene50248:CDS 40.0%
GAACATCTATAGAGATCTCC+TGG + chr5.2:61324503-61324522 None:intergenic 40.0%
GAATGATTTCAAACCTTGGC+TGG + chr5.2:61324525-61324544 None:intergenic 40.0%
GACCATTCGACCTTTATCAA+TGG - chr5.2:61324177-61324196 MS.gene50248:CDS 40.0%
GCACTAATCCTAATAGTCCA+AGG - chr5.2:61325240-61325259 MS.gene50248:intron 40.0%
GCATTCACATCAAGGATTAG+AGG - chr5.2:61325143-61325162 MS.gene50248:intron 40.0%
GCTGCAAATCTTGTTCCATT+TGG + chr5.2:61325275-61325294 None:intergenic 40.0%
GGACATTGTGTCACATAACT+AGG + chr5.2:61323766-61323785 None:intergenic 40.0%
GTGATCCAAGTAACAAATGC+TGG - chr5.2:61323550-61323569 MS.gene50248:intron 40.0%
GTGGTCGATTGGATCAAAAT+TGG - chr5.2:61324090-61324109 MS.gene50248:CDS 40.0%
GTTCCAATAGTATTCCTCAC+AGG + chr5.2:61325644-61325663 None:intergenic 40.0%
GTTGTCAAATGCAGCATTCA+TGG + chr5.2:61325027-61325046 None:intergenic 40.0%
GTTTGTCTTAAGAGAGCCAT+TGG + chr5.2:61324425-61324444 None:intergenic 40.0%
TACACAGGAAATGTTCCTAG+GGG - chr5.2:61325416-61325435 MS.gene50248:intron 40.0%
TAGCAACAACAGTCATTCTG+TGG + chr5.2:61324872-61324891 None:intergenic 40.0%
TATCATGAGGACTCTAGTGT+TGG + chr5.2:61324919-61324938 None:intergenic 40.0%
TCTGTTGCATACACCATGAA+CGG - chr5.2:61324473-61324492 MS.gene50248:CDS 40.0%
TTCAAACTGCAACAGTGAAG+AGG - chr5.2:61323446-61323465 MS.gene50248:intron 40.0%
TTCACATCTCAATTGGGGTT+TGG - chr5.2:61326038-61326057 MS.gene50248:intron 40.0%
TTGAACTGTATAGGAGGAAC+AGG + chr5.2:61325395-61325414 None:intergenic 40.0%
TTTATCAATGGCCACTGATG+TGG - chr5.2:61324189-61324208 MS.gene50248:CDS 40.0%
! AAGGTCCAGCATTTGTTACT+TGG + chr5.2:61323558-61323577 None:intergenic 40.0%
! ACCTTCAAGACCTATTTTGC+CGG - chr5.2:61325085-61325104 MS.gene50248:intron 40.0%
! AGGACATTGATTGTTTGACC+TGG + chr5.2:61324945-61324964 None:intergenic 40.0%
! GAGGTATCACTGACTGTAAT+GGG + chr5.2:61326095-61326114 None:intergenic 40.0%
! GGATAAGGAGAACCCATTTT+TGG + chr5.2:61323910-61323929 None:intergenic 40.0%
! GTCTTGAAGGTGGTCTATTT+TGG + chr5.2:61325076-61325095 None:intergenic 40.0%
! GTTGGCTTAGAGAAAGGATA+AGG + chr5.2:61323925-61323944 None:intergenic 40.0%
! TCTCTAGTTGGCTTAGAGAA+AGG + chr5.2:61323931-61323950 None:intergenic 40.0%
AACGTAGCAGGATCAGTTCT+TGG + chr5.2:61325605-61325624 None:intergenic 45.0%
AACTGTATAGGAGGAACAGG+AGG + chr5.2:61325392-61325411 None:intergenic 45.0%
ACACAATGTCCAATCCAACC+AGG - chr5.2:61323775-61323794 MS.gene50248:CDS 45.0%
ACCAAGAAGGAACACTATGG+TGG - chr5.2:61323830-61323849 MS.gene50248:CDS 45.0%
ACCGTAAATGGACAGTTTCC+TGG - chr5.2:61323493-61323512 MS.gene50248:intron 45.0%
AGGAACAGGAGGAAAATCTG+TGG + chr5.2:61325381-61325400 None:intergenic 45.0%
ATCGTCACTCGTCCTCATTA+TGG - chr5.2:61323606-61323625 MS.gene50248:intron 45.0%
ATGTGTAACTTCCTCCTGGT+TGG + chr5.2:61323792-61323811 None:intergenic 45.0%
CACATATGCCAGTGTCGTAT+TGG + chr5.2:61325770-61325789 None:intergenic 45.0%
CAGTGGATGGAGATGTTGTA+AGG + chr5.2:61323577-61323596 None:intergenic 45.0%
CCAATAGTATTCCTCACAGG+TGG + chr5.2:61325641-61325660 None:intergenic 45.0%
CCACCTGTGAGGAATACTAT+TGG - chr5.2:61325638-61325657 MS.gene50248:CDS 45.0%
CCTTGACACCTTGGACTATT+AGG + chr5.2:61325251-61325270 None:intergenic 45.0%
CGAAGGATACTCACCGTAAA+TGG - chr5.2:61323481-61323500 MS.gene50248:intron 45.0%
CGATGATAGTTACCAGTGGA+TGG + chr5.2:61323590-61323609 None:intergenic 45.0%
CTCTATAGATGTTCCAGCCA+AGG - chr5.2:61324509-61324528 MS.gene50248:CDS 45.0%
CTGCAACTGCATTCACATCA+AGG - chr5.2:61325135-61325154 MS.gene50248:intron 45.0%
CTTACAACATCTCCATCCAC+TGG - chr5.2:61323575-61323594 MS.gene50248:intron 45.0%
GAAATGTTCCTAGGGGTTTG+TGG - chr5.2:61325423-61325442 MS.gene50248:intron 45.0%
GATCACCCTATGCATGTTCA+TGG - chr5.2:61325536-61325555 MS.gene50248:CDS 45.0%
GCCAGGAAACTGTCCATTTA+CGG + chr5.2:61323497-61323516 None:intergenic 45.0%
GCTTACTACAATGGTGTTCC+TGG - chr5.2:61325347-61325366 MS.gene50248:intron 45.0%
GGAACCCATGAACATGCATA+GGG + chr5.2:61325544-61325563 None:intergenic 45.0%
GGCAAAATAGGTCTTGAAGG+TGG + chr5.2:61325086-61325105 None:intergenic 45.0%
GGCCATTGATAAAGGTCGAA+TGG + chr5.2:61324182-61324201 None:intergenic 45.0%
GGTTCTATAGCTAGAGCACA+TGG + chr5.2:61325749-61325768 None:intergenic 45.0%
GGTTTGTGGACACCTAGAAA+AGG - chr5.2:61325437-61325456 MS.gene50248:intron 45.0%
GTCAAATGCAGCATTCATGG+CGG + chr5.2:61325024-61325043 None:intergenic 45.0%
GTGACGATGATAGTTACCAG+TGG + chr5.2:61323594-61323613 None:intergenic 45.0%
TAACTTCCTCCTGGTTGGAT+TGG + chr5.2:61323787-61323806 None:intergenic 45.0%
TGATGTGAATGCAGTTGCAG+TGG + chr5.2:61325135-61325154 None:intergenic 45.0%
TGGAACCCATGAACATGCAT+AGG + chr5.2:61325545-61325564 None:intergenic 45.0%
TGTCTTTGTGCAACAGGCAT+GGG - chr5.2:61323707-61323726 MS.gene50248:CDS 45.0%
TTCCTAAGAGCCACTGTCTA+TGG - chr5.2:61323868-61323887 MS.gene50248:CDS 45.0%
TTGAGAAATCCATGGGCAGA+TGG - chr5.2:61323739-61323758 MS.gene50248:CDS 45.0%
TTTGACCTGGACCTATCATG+AGG + chr5.2:61324932-61324951 None:intergenic 45.0%
! ATGAGGACTCTAGTGTTGGA+AGG + chr5.2:61324915-61324934 None:intergenic 45.0%
! CCTAATAGTCCAAGGTGTCA+AGG - chr5.2:61325248-61325267 MS.gene50248:intron 45.0%
! GTGGCCGTGTCGTTAAAAAA+TGG + chr5.2:61325116-61325135 None:intergenic 45.0%
! TAGCATTGAGGTAGATCTGG+TGG + chr5.2:61326120-61326139 None:intergenic 45.0%
! TGTTTCATTTGCTGCAGCAG+AGG - chr5.2:61323312-61323331 MS.gene50248:CDS 45.0%
!! GGAGGTATCACTGACTGTAA+TGG + chr5.2:61326096-61326115 None:intergenic 45.0%
ACTATTGGAACACCTCCTGG+TGG - chr5.2:61325653-61325672 MS.gene50248:CDS 50.0%
AGAGGCTGTGCAAAACACGA+AGG - chr5.2:61323464-61323483 MS.gene50248:intron 50.0%
AGAGTCCTCATGATAGGTCC+AGG - chr5.2:61324924-61324943 MS.gene50248:CDS 50.0%
ATCTCCTGGTTGACCGTTCA+TGG + chr5.2:61324489-61324508 None:intergenic 50.0%
ATGCAGCATTCATGGCGGTT+TGG + chr5.2:61325019-61325038 None:intergenic 50.0%
ATGGGTCGCCATTCGTTTCA+AGG - chr5.2:61325676-61325695 MS.gene50248:intron 50.0%
CAATGTCCAATCCAACCAGG+AGG - chr5.2:61323778-61323797 MS.gene50248:CDS 50.0%
CATTGAGGTAGATCTGGTGG+TGG + chr5.2:61326117-61326136 None:intergenic 50.0%
CCATTTGGTCCTTGACACCT+TGG + chr5.2:61325260-61325279 None:intergenic 50.0%
CGGTATGTGTAACTTCCTCC+TGG + chr5.2:61323796-61323815 None:intergenic 50.0%
CGTTTCAAGGCCGATAATCC+AGG - chr5.2:61325689-61325708 MS.gene50248:intron 50.0%
CTATGGTGGCATGCTCATAC+TGG - chr5.2:61323844-61323863 MS.gene50248:CDS 50.0%
CTCCATAGACAGTGGCTCTT+AGG + chr5.2:61323873-61323892 None:intergenic 50.0%
GATCCAATCGACCACGGATA+TGG + chr5.2:61324085-61324104 None:intergenic 50.0%
GCCACCATAGTGTTCCTTCT+TGG + chr5.2:61323834-61323853 None:intergenic 50.0%
GCCGGCAAAATAGGTCTTGA+AGG + chr5.2:61325089-61325108 None:intergenic 50.0%
GTGTCTTTGTGCAACAGGCA+TGG - chr5.2:61323706-61323725 MS.gene50248:CDS 50.0%
TAACTAGGTCCATCTGCCCA+TGG + chr5.2:61323751-61323770 None:intergenic 50.0%
TACACCATGAACGGTCAACC+AGG - chr5.2:61324482-61324501 MS.gene50248:CDS 50.0%
TAGCCATATCCGTGGTCGAT+TGG - chr5.2:61324079-61324098 MS.gene50248:CDS 50.0%
TCGACAAGGTTGAACGTAGC+AGG + chr5.2:61325617-61325636 None:intergenic 50.0%
! AGCGAGGGACGGAGATTTTA+TGG - chr5.2:61323528-61323547 MS.gene50248:intron 50.0%
! CCAAGGTGTCAAGGACCAAA+TGG - chr5.2:61325257-61325276 MS.gene50248:intron 50.0%
! GGGTTTGGCAACAGCACTTT+TGG - chr5.2:61326053-61326072 MS.gene50248:intron 50.0%
ACCACCTGGCCGATACTACA+TGG - chr5.2:61324980-61324999 MS.gene50248:CDS 55.0%
ACCTTGTCGATCCACCTGTG+AGG - chr5.2:61325627-61325646 MS.gene50248:CDS 55.0%
CGCGTGCTGCCATGTAGTAT+CGG + chr5.2:61324992-61325011 None:intergenic 55.0%
CTAGGTGTCCACAAACCCCT+AGG + chr5.2:61325434-61325453 None:intergenic 55.0%
GTGGTTGATCGGCTGTGACA+AGG + chr5.2:61324965-61324984 None:intergenic 55.0%
TCCTCACAGGTGGATCGACA+AGG + chr5.2:61325631-61325650 None:intergenic 55.0%
TGTGCAACAGGCATGGGCTT+AGG - chr5.2:61323713-61323732 MS.gene50248:CDS 55.0%
TTGGAACACCTCCTGGTGGA+TGG - chr5.2:61325657-61325676 MS.gene50248:CDS 55.0%
! GTATCGGCCAGGTGGTTGAT+CGG + chr5.2:61324976-61324995 None:intergenic 55.0%
! TGGAACACCTCCTGGTGGAT+GGG - chr5.2:61325658-61325677 MS.gene50248:CDS 55.0%
!! TGAGGTAGATCTGGTGGTGG+AGG + chr5.2:61326114-61326133 None:intergenic 55.0%
CACGCAGCTAGCCATATCCG+TGG - chr5.2:61324071-61324090 MS.gene50248:CDS 60.0%
CACGCAGTGAGCCACATCAG+TGG + chr5.2:61324203-61324222 None:intergenic 60.0%
CCAACGATCGAAGCGAGGGA+CGG - chr5.2:61323517-61323536 MS.gene50248:intron 60.0%
CGTCCCTCGCTTCGATCGTT+GGG + chr5.2:61323519-61323538 None:intergenic 60.0%
GTCACAGCCGATCAACCACC+TGG - chr5.2:61324966-61324985 MS.gene50248:CDS 60.0%
TGGCCCAACGATCGAAGCGA+GGG - chr5.2:61323513-61323532 MS.gene50248:intron 60.0%
! CACGGATATGGCTAGCTGCG+TGG + chr5.2:61324073-61324092 None:intergenic 60.0%
!! GCCATGTAGTATCGGCCAGG+TGG + chr5.2:61324984-61325003 None:intergenic 60.0%
!! GCTGCCATGTAGTATCGGCC+AGG + chr5.2:61324987-61325006 None:intergenic 60.0%
ATGGCGACCCATCCACCAGG+AGG + chr5.2:61325668-61325687 None:intergenic 65.0%
CCGTCCCTCGCTTCGATCGT+TGG + chr5.2:61323520-61323539 None:intergenic 65.0%
CGAATGGCGACCCATCCACC+AGG + chr5.2:61325671-61325690 None:intergenic 65.0%
CTCGCTTCGATCGTTGGGCC+AGG + chr5.2:61323514-61323533 None:intergenic 65.0%
CTGGCCCAACGATCGAAGCG+AGG - chr5.2:61323512-61323531 MS.gene50248:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 61323268 61326140 61323268 ID=MS.gene50248
chr5.2 mRNA 61323268 61326140 61323268 ID=MS.gene50248.t1;Parent=MS.gene50248
chr5.2 exon 61326054 61326140 61326054 ID=MS.gene50248.t1.exon1;Parent=MS.gene50248.t1
chr5.2 CDS 61326054 61326140 61326054 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
chr5.2 exon 61325812 61325963 61325812 ID=MS.gene50248.t1.exon2;Parent=MS.gene50248.t1
chr5.2 CDS 61325812 61325963 61325812 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
chr5.2 exon 61325441 61325685 61325441 ID=MS.gene50248.t1.exon3;Parent=MS.gene50248.t1
chr5.2 CDS 61325441 61325685 61325441 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
chr5.2 exon 61324878 61325006 61324878 ID=MS.gene50248.t1.exon4;Parent=MS.gene50248.t1
chr5.2 CDS 61324878 61325006 61324878 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
chr5.2 exon 61323698 61324633 61323698 ID=MS.gene50248.t1.exon5;Parent=MS.gene50248.t1
chr5.2 CDS 61323698 61324633 61323698 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
chr5.2 exon 61323268 61323398 61323268 ID=MS.gene50248.t1.exon6;Parent=MS.gene50248.t1
chr5.2 CDS 61323268 61323398 61323268 ID=cds.MS.gene50248.t1;Parent=MS.gene50248.t1
Gene Sequence

>MS.gene50248

ATGAAGACTATTTTATGCTACTTACTTGGCTTACTTACTATCATTGTTTCATTTGCTGCAGCAGAGGAGCATTACCATCAGTTTGTTGTAAGTGTTTGTTTGATGATCACATTCATATATATATGTTTGTACCAATTTGCAACTGTATTAACTAACTTTAATGATTGCTGGAAATAGATTCAAACTGCAACAGTGAAGAGGCTGTGCAAAACACGAAGGATACTCACCGTAAATGGACAGTTTCCTGGCCCAACGATCGAAGCGAGGGACGGAGATTTTATGGTGATCCAAGTAACAAATGCTGGACCTTACAACATCTCCATCCACTGGTAACTATCATCGTCACTCGTCCTCATTATGGTCTTAAATACTTGTATGATAAGCGCTTAATTAACCCGTTTATCCAAACATTCACTATGTTCCTGACAATCTTCTTATGTGTCTTTGTGCAACAGGCATGGGCTTAGGATGTTGAGAAATCCATGGGCAGATGGACCTAGTTATGTGACACAATGTCCAATCCAACCAGGAGGAAGTTACACATACCGATTTACAATTCAAAACCAAGAAGGAACACTATGGTGGCATGCTCATACTGGTTTCCTAAGAGCCACTGTCTATGGAGCTTTCATTATTTATCCAAAAATGGGTTCTCCTTATCCTTTCTCTAAGCCAACTAGAGAATTTCCCGTTCTTCTTGGTAATCTTCAAAACTATCATCATTTTATTATACTTAAGAGAGCTAGTTAGATAAACCGTTCAAAATAGGATTTGAATTGTGTGCAGTAACAATCTCATGCAGCCACGCAGCTAGCCATATCCGTGGTCGATTGGATCAAAATTGGACGGTTCAAATTTAAATTCAGATTTGGTTATTTTTCAGAAACTAAATACCAAGTTTAAAATATGGACCATTCGACCTTTATCAATGGCCACTGATGTGGCTCACTGCGTGACTATATAGCATTGCGATTGCACACAACCCAAATCCCTAAAAAAATACTCAAACAAATCTTACAATGATTTAGAAACTCTCTAGTTTAACTTCTTAATAAACACTTCAAAAGTTGCTCTTAAATTTTTATGCAACAAAGACAGAGCCATATTGACAGAATATGTTTTTATGATGCATAGGGGAATGGTTTGATAGAGATCCAATGGCTCTCTTAAGACAAACACAGTTCACAGGAGCACCTCCAAATGTATCTGTTGCATACACCATGAACGGTCAACCAGGAGATCTCTATAGATGTTCCAGCCAAGGTTTGAAATCATTCTAAATCGCCATTACTTCCATACATCGTTTTAAATTGCAGCTGGAGTCGCAGTTACACTGCAAACCTTTATATTACAGAAAAATATAGATGATGCTGTCAAAATTATGATTGCGATATTGTTGCAAAAAACTCAATCCTTTATATTACGGTTGCAGACAATAATTTAGAACTCATTTTTCGTACTCGTTGATAAAAAAGTGACTAAAGGTATAAAAACAATTACAATGCAGGAACCGTACGTTTTCAAGTATATGTCGGAGAAACAATTCTCTTAAGAATCATCAACAGTGCACTCAATCAAGAACTCTTCTTTTCAATTGCTAACCACAGAATGACTGTTGTTGCTACGGATGCTGCTTATACAAAACCTTCCAACACTAGAGTCCTCATGATAGGTCCAGGTCAAACAATCAATGTCCTTGTCACAGCCGATCAACCACCTGGCCGATACTACATGGCAGCACGCGCTTACCAAACCGCCATGAATGCTGCATTTGACAACACGACGACAACTGCAATTCTTGAATACCAAAATAGACCACCTTCAAGACCTATTTTGCCGGCTTTACCATTTTTTAACGACACGGCCACTGCAACTGCATTCACATCAAGGATTAGAGGACTTTCCAAAATTAAAGTGTTTCGAAATGTTGATGTGAGTTTATTGTTCACTGTAGGGTTAGGATTAATAAACTGCACTAATCCTAATAGTCCAAGGTGTCAAGGACCAAATGGAACAAGATTTGCAGCTAGTATAAACAACAACTCTTTTGTTCTTCCTAGAACAACTTCCTTAATGCAAGCTTACTACAATGGTGTTCCTGGTGTTTTTACCACAGATTTTCCTCCTGTTCCTCCTATACAGTTCAATTACACAGGAAATGTTCCTAGGGGTTTGTGGACACCTAGAAAAGGAACCAAGTTGTTCAAGTTGAAATATGGTTCCAATGTTCAAATTGTTTTGCAGGATACTAGTATAGTAACAATTGAGGATCACCCTATGCATGTTCATGGGTTCCATTTCTTTATAGTTGGTTCAGGTTTTGGAAATTTTAATCCAAGAACTGATCCTGCTACGTTCAACCTTGTCGATCCACCTGTGAGGAATACTATTGGAACACCTCCTGGTGGATGGGTCGCCATTCGTTTCAAGGCCGATAATCCAGGTATAATGCATTTATGTTACTCATATACAAAGATGCCATGTGCTCTAGCTATAGAACCAATACGACACTGGCATATGTGATATCGTTCAATTAATTCATTTTTTTTTAAAAATTATTACTGATGTCAACGTATCAGTATCATGTCTGATGTTTGTGTCAGTGTTTTATAGGTCATGTGAATGTTTTTAAATCATGGTCGCGGTGTCCGATATCTCTCTCTTGCATAATTTATACTAGGCTCTTAGCATTGTATGTATACTAACCAAATGTTGATTTTTGAATTCATTGTTGTGAATCAGGAATTTGGTTCTTGCATTGTCACATAGATTCACATCTCAATTGGGGTTTGGCAACAGCACTTTTGGTAGAAAATGGAGTTGGCCCATTACAGTCAGTGATACCTCCACCACCAGATCTACCTCAATGCTAA
Protein sequence

>MS.gene50248.t1

MKTILCYLLGLLTIIVSFAAAEEHYHQFVIQTATVKRLCKTRRILTVNGQFPGPTIEARDGDFMVIQVTNAGPYNISIHWHGLRMLRNPWADGPSYVTQCPIQPGGSYTYRFTIQNQEGTLWWHAHTGFLRATVYGAFIIYPKMGSPYPFSKPTREFPVLLGEWFDRDPMALLRQTQFTGAPPNVSVAYTMNGQPGDLYRCSSQGTVRFQVYVGETILLRIINSALNQELFFSIANHRMTVVATDAAYTKPSNTRVLMIGPGQTINVLVTADQPPGRYYMAARAYQTAMNAAFDNTTTTAILEYQNRPPSRPILPALPFFNDTATATAFTSRIRGLSKIKVFRNVDVSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVLPRTTSLMQAYYNGVPGVFTTDFPPVPPIQFNYTGNVPRGLWTPRKGTKLFKLKYGSNVQIVLQDTSIVTIEDHPMHVHGFHFFIVGSGFGNFNPRTDPATFNLVDPPVRNTIGTPPGGWVAIRFKADNPGIWFLHCHIDSHLNWGLATALLVENGVGPLQSVIPPPPDLPQC