AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85144.t1 MTR_2g101330 96.667 330 10 1 1 329 1 330 0.0 662
MS.gene85144.t1 MTR_3g005210 43.354 316 171 6 5 316 6 317 1.15e-86 263
MS.gene85144.t1 MTR_5g056600 43.218 317 175 4 6 319 5 319 3.79e-85 259
MS.gene85144.t1 MTR_3g005170 42.368 321 177 6 5 321 10 326 7.00e-83 254
MS.gene85144.t1 MTR_8g062110 42.500 320 167 6 5 316 9 319 4.27e-81 249
MS.gene85144.t1 MTR_8g062110 42.500 320 167 6 5 316 18 328 9.67e-81 249
MS.gene85144.t1 MTR_0164s0070 90.840 131 12 0 1 131 1 131 1.22e-80 244
MS.gene85144.t1 MTR_0164s0070 83.099 71 12 0 206 276 122 192 9.15e-37 131
MS.gene85144.t1 MTR_1g022440 39.875 321 184 6 5 319 8 325 2.43e-79 245
MS.gene85144.t1 MTR_3g005170 45.956 272 143 4 5 275 10 278 2.64e-79 243
MS.gene85144.t1 MTR_8g062440 41.066 319 179 5 5 319 8 321 8.36e-79 243
MS.gene85144.t1 MTR_8g062440 41.250 320 178 6 5 319 8 322 4.19e-78 242
MS.gene85144.t1 MTR_5g072620 40.248 323 187 4 1 320 1 320 2.87e-77 239
MS.gene85144.t1 MTR_4g009040 40.625 320 178 5 4 319 12 323 8.45e-77 238
MS.gene85144.t1 MTR_8g062110 44.891 274 138 4 5 273 9 274 4.75e-76 235
MS.gene85144.t1 MTR_8g062110 44.891 274 138 4 5 273 9 274 8.23e-76 234
MS.gene85144.t1 MTR_8g062440 44.444 270 145 3 5 273 8 273 7.05e-75 233
MS.gene85144.t1 MTR_1g022445 38.344 326 190 7 1 319 4 325 8.03e-75 234
MS.gene85144.t1 MTR_4g009040 40.123 324 178 6 4 319 12 327 1.87e-74 233
MS.gene85144.t1 MTR_4g077130 40.506 316 180 6 5 316 9 320 4.94e-74 231
MS.gene85144.t1 MTR_4g077100 39.936 313 180 6 5 313 11 319 5.14e-74 231
MS.gene85144.t1 MTR_4g077100 42.804 271 151 4 5 274 11 278 2.93e-72 225
MS.gene85144.t1 MTR_2g104960 40.867 323 180 5 5 324 20 334 1.75e-71 225
MS.gene85144.t1 MTR_4g092080 38.110 328 189 8 2 317 11 336 5.71e-70 221
MS.gene85144.t1 MTR_3g031650 37.812 320 193 4 1 317 1 317 6.47e-70 221
MS.gene85144.t1 MTR_4g009690 40.439 319 179 5 4 319 12 322 1.66e-69 219
MS.gene85144.t1 MTR_4g006940 39.506 324 185 5 2 322 14 329 4.32e-69 219
MS.gene85144.t1 MTR_7g074880 39.877 326 180 10 4 319 7 326 7.49e-69 218
MS.gene85144.t1 MTR_7g074730 39.450 327 182 10 4 319 7 328 1.07e-68 218
MS.gene85144.t1 MTR_3g031650 40.364 275 162 2 1 275 1 273 1.12e-68 216
MS.gene85144.t1 MTR_4g009690 40.000 320 180 5 4 319 12 323 2.20e-68 217
MS.gene85144.t1 MTR_7g074820 39.939 328 180 10 4 319 7 329 5.11e-68 216
MS.gene85144.t1 MTR_7g074850 38.720 328 184 9 4 319 7 329 1.90e-67 214
MS.gene85144.t1 MTR_3g005170 37.383 321 162 6 5 321 10 295 7.79e-65 207
MS.gene85144.t1 MTR_7g074710 39.877 326 180 9 4 319 7 326 1.76e-64 207
MS.gene85144.t1 MTR_4g006940 41.392 273 152 4 2 274 14 278 4.62e-61 196
MS.gene85144.t1 MTR_4g006940 41.392 273 152 4 2 274 14 278 4.62e-61 196
MS.gene85144.t1 MTR_2g028620 35.671 328 193 9 2 319 7 326 1.38e-60 197
MS.gene85144.t1 MTR_4g081440 35.608 337 190 9 4 319 19 349 2.59e-60 197
MS.gene85144.t1 MTR_4g081440 35.988 339 187 11 4 319 23 354 5.82e-59 193
MS.gene85144.t1 MTR_2g028620 38.768 276 161 5 2 273 7 278 1.46e-58 191
MS.gene85144.t1 MTR_4g023730 33.742 326 201 8 5 319 8 329 1.26e-52 176
MS.gene85144.t1 MTR_7g074870 33.898 295 172 10 38 319 4 288 1.30e-45 157
MS.gene85144.t1 MTR_8g062110 35.931 231 132 5 94 316 11 233 3.76e-41 144
MS.gene85144.t1 MTR_8g062110 37.838 185 103 3 94 273 11 188 3.99e-36 129
MS.gene85144.t1 MTR_5g029990 30.827 266 168 5 3 267 10 260 2.78e-34 127
MS.gene85144.t1 MTR_7g117190 30.564 337 211 11 4 319 6 340 4.72e-34 128
MS.gene85144.t1 MTR_6g406250 28.716 296 197 7 5 292 55 344 1.47e-31 121
MS.gene85144.t1 MTR_7g117190 31.169 308 187 11 4 291 6 308 1.65e-31 120
MS.gene85144.t1 MTR_2g013230 28.319 339 219 9 4 321 7 342 3.83e-31 120
MS.gene85144.t1 MTR_2g013250 27.914 326 211 8 4 308 6 328 8.41e-31 119
MS.gene85144.t1 MTR_2g013260 28.354 328 207 9 4 308 7 329 3.55e-28 112
MS.gene85144.t1 MTR_2g013260 29.452 292 179 8 4 273 7 293 3.31e-27 108
MS.gene85144.t1 MTR_6g406250 31.771 192 127 2 5 193 55 245 3.57e-25 102
MS.gene85144.t1 MTR_3g011130 40.517 116 67 2 97 210 30 145 6.14e-19 82.4
MS.gene85144.t1 MTR_0685s0030 31.013 158 103 4 159 313 2 156 1.29e-18 82.0
MS.gene85144.t1 MTR_0685s0030 34.483 116 74 2 159 274 2 115 6.66e-17 75.9
MS.gene85144.t1 MTR_8g062710 42.105 95 44 2 4 98 19 102 1.04e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85144.t1 AT4G35420 70.978 317 91 1 4 320 7 322 7.94e-175 488
MS.gene85144.t1 AT1G68540 42.587 317 177 4 7 319 6 321 2.29e-83 255
MS.gene85144.t1 AT1G51410 43.614 321 175 5 2 319 6 323 8.27e-83 254
MS.gene85144.t1 AT5G19440 42.767 318 172 5 5 317 10 322 6.17e-81 249
MS.gene85144.t1 AT5G58490 41.379 319 181 4 5 320 9 324 1.11e-78 243
MS.gene85144.t1 AT1G09480 41.139 316 178 5 5 316 8 319 1.20e-78 243
MS.gene85144.t1 AT1G09480 41.139 316 178 5 5 316 55 366 2.40e-78 244
MS.gene85144.t1 AT5G42800 39.815 324 186 7 2 319 5 325 6.69e-76 238
MS.gene85144.t1 AT1G25460 43.396 318 171 7 7 319 6 319 7.17e-76 236
MS.gene85144.t1 AT1G09490 40.635 315 181 4 5 316 8 319 1.62e-75 235
MS.gene85144.t1 AT1G66800 40.764 314 178 5 5 316 8 315 9.52e-75 233
MS.gene85144.t1 AT1G09510 41.270 315 179 5 5 316 8 319 2.76e-74 232
MS.gene85144.t1 AT2G02400 38.994 318 186 5 5 319 6 318 1.79e-73 229
MS.gene85144.t1 AT1G09500 38.801 317 187 4 5 316 8 322 2.22e-71 224
MS.gene85144.t1 AT1G09500 41.852 270 154 2 5 273 8 275 5.99e-70 219
MS.gene85144.t1 AT1G61720 38.650 326 186 7 4 317 12 335 1.15e-68 218
MS.gene85144.t1 AT2G33590 38.387 310 188 3 4 313 8 314 2.23e-64 206
MS.gene85144.t1 AT1G15950 38.994 318 183 6 5 319 13 322 3.54e-63 204
MS.gene85144.t1 AT1G15950 38.994 318 183 6 5 319 13 322 3.54e-63 204
MS.gene85144.t1 AT1G76470 39.938 323 187 5 1 319 3 322 6.09e-63 203
MS.gene85144.t1 AT1G80820 37.578 322 190 6 5 323 8 321 1.57e-62 202
MS.gene85144.t1 AT2G33600 38.065 310 189 3 4 313 8 314 4.80e-60 195
MS.gene85144.t1 AT1G15950 38.762 307 177 6 5 308 13 311 2.37e-59 194
MS.gene85144.t1 AT1G09490 37.722 281 169 4 39 316 11 288 1.39e-58 191
MS.gene85144.t1 AT2G45400 36.364 330 190 7 5 319 40 364 1.09e-57 191
MS.gene85144.t1 AT1G15950 40.659 273 154 5 5 277 13 277 2.45e-56 185
MS.gene85144.t1 AT1G15950 40.217 276 157 5 5 280 13 280 9.69e-56 183
MS.gene85144.t1 AT1G15950 40.741 270 152 5 5 274 13 274 1.33e-55 182
MS.gene85144.t1 AT1G09500 36.396 283 173 4 39 316 8 288 4.04e-55 182
MS.gene85144.t1 AT4G27250 33.429 347 198 10 6 326 14 353 5.58e-54 181
MS.gene85144.t1 AT2G45400 37.410 278 165 4 5 274 40 316 9.02e-54 179
MS.gene85144.t1 AT1G80820 39.259 270 156 5 5 274 8 269 1.31e-53 177
MS.gene85144.t1 AT4G27250 33.529 340 197 10 6 326 14 343 1.94e-53 179
MS.gene85144.t1 AT1G68540 40.000 215 124 4 109 319 1 214 3.37e-47 159
MS.gene85144.t1 AT2G45400 33.108 296 180 6 37 319 17 307 6.88e-44 153
MS.gene85144.t1 AT2G23910 27.839 273 188 5 6 277 11 275 5.33e-38 137
MS.gene85144.t1 AT2G45400 37.963 216 125 4 5 212 40 254 7.63e-38 135
MS.gene85144.t1 AT2G23910 28.148 270 185 5 6 274 11 272 1.50e-37 135
MS.gene85144.t1 AT2G23910 28.000 275 189 5 6 279 11 277 1.94e-37 136
MS.gene85144.t1 AT1G15950 34.071 226 144 3 97 319 1 224 3.52e-34 125
MS.gene85144.t1 AT4G30470 26.909 275 189 6 6 278 11 275 3.61e-31 119
MS.gene85144.t1 AT2G23910 25.896 251 177 5 30 279 1 243 3.18e-28 110
MS.gene85144.t1 AT5G14700 28.788 264 173 6 5 258 55 313 1.16e-26 108
MS.gene85144.t1 AT5G14700 28.704 216 143 4 5 213 55 266 2.85e-19 86.7
MS.gene85144.t1 AT4G33360 25.993 277 180 10 4 274 14 271 1.18e-13 70.9
MS.gene85144.t1 AT4G33360 25.271 277 182 9 4 274 2 259 1.32e-13 70.9
MS.gene85144.t1 AT4G33360 25.271 277 182 9 4 274 14 271 1.67e-13 70.9

Find 69 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGAAAGTTGAACATTTA+TGG 0.118270 2.4:+3421663 MS.gene85144:CDS
ATTGGAACAGTTAGAGATTT+AGG 0.193478 2.4:+3421543 MS.gene85144:CDS
ACTAGTCCAAGCTGATTTAA+TGG 0.234242 2.4:+3421715 MS.gene85144:CDS
CATAAATCTGTTGGTAAGTT+AGG 0.314397 2.4:-3422362 None:intergenic
CTTTGCATCGTTTGTGGAGC+AGG 0.328688 2.4:+3423229 MS.gene85144:CDS
ACTGGAAAGCTGGAGAGTTT+AGG 0.349228 2.4:+3423167 MS.gene85144:CDS
AGTTGAACATTTATGGAAGT+TGG 0.360831 2.4:+3421670 MS.gene85144:CDS
GTGTGTGTGACAGGAGCTTC+AGG 0.361806 2.4:+3421468 MS.gene85144:CDS
CTGCCAGTGTCTTTGACATT+GGG 0.362452 2.4:-3422265 None:intergenic
AATAATGGATAATGATGATT+TGG 0.363243 2.4:+3422632 MS.gene85144:CDS
TCTGCCAGTGTCTTTGACAT+TGG 0.367364 2.4:-3422266 None:intergenic
TAGCTTCGACAACGCGATCA+TGG 0.382636 2.4:+3421745 MS.gene85144:CDS
TTGTCTGGATATCATGTCAT+TGG 0.385389 2.4:+3421525 MS.gene85144:CDS
CGAGTCATCTTGGAGCTCAT+TGG 0.428944 2.4:+3422132 MS.gene85144:CDS
TCAGGTTCAAGAAACTAGAT+AGG 0.430756 2.4:+3423126 MS.gene85144:intron
TGAACCAACCCTTTGGGAAT+GGG 0.449618 2.4:-3422687 None:intergenic
TTTCTTCCATTAAATCAGCT+TGG 0.450929 2.4:-3421721 None:intergenic
TGATTGCTTTGCATCGTTTG+TGG 0.452335 2.4:+3423223 MS.gene85144:CDS
CTCCAAGATGACTCGTCTAA+TGG 0.454787 2.4:-3422124 None:intergenic
TAACACAGCACGGGGTTGGT+GGG 0.455832 2.4:-3423267 None:intergenic
TACCATTAGACGAGTCATCT+TGG 0.465869 2.4:+3422122 MS.gene85144:CDS
TGTCTTTCACATTGCCTCTC+CGG 0.466059 2.4:+3421778 MS.gene85144:CDS
AGAAATTTCAATGGCATGGA+AGG 0.468896 2.4:+3422517 MS.gene85144:CDS
ATGAACCAACCCTTTGGGAA+TGG 0.471597 2.4:-3422688 None:intergenic
GAAGCATGAACCAACCCTTT+GGG 0.473604 2.4:-3422693 None:intergenic
AACACAACTCGAACAAGGGC+TGG 0.477786 2.4:-3422049 None:intergenic
TGAACTAAACACTGGAAAGC+TGG 0.486679 2.4:+3423157 MS.gene85144:CDS
AGCTTCGACAACGCGATCAT+GGG 0.489980 2.4:+3421746 MS.gene85144:CDS
ATGCTAGCAAATCGTTATCC+TGG 0.493557 2.4:+3422660 MS.gene85144:CDS
TTCAGCGTACCTTGGACCGC+TGG 0.497416 2.4:-3422001 None:intergenic
TGGGAATATTGCAAAGAGAA+TGG 0.500498 2.4:+3422299 MS.gene85144:CDS
ATTTCAATGGCATGGAAGGA+TGG 0.500563 2.4:+3422521 MS.gene85144:CDS
TTAGGTCCAATTATGAATGA+TGG 0.503563 2.4:-3422344 None:intergenic
TTTCAATGGCATGGAAGGAT+GGG 0.514191 2.4:+3422522 MS.gene85144:CDS
ACTGAGAAATTTCAATGGCA+TGG 0.516718 2.4:+3422513 MS.gene85144:CDS
ACAATAACACAGCACGGGGT+TGG 0.517165 2.4:-3423271 None:intergenic
AACACAGCACGGGGTTGGTG+GGG 0.518483 2.4:-3423266 None:intergenic
CCAGTGTTTAGTTCATAATG+TGG 0.519090 2.4:-3423149 None:intergenic
TCACATATCAAATGATCCTA+AGG 0.523302 2.4:+3421808 MS.gene85144:CDS
ATAACACAGCACGGGGTTGG+TGG 0.523703 2.4:-3423268 None:intergenic
TCCACTGCATCTGATGTGTT+AGG 0.524578 2.4:+3422386 MS.gene85144:CDS
CAAAGAGAATGGAATAGACT+TGG 0.535237 2.4:+3422310 MS.gene85144:CDS
GAACATTTATGGAAGTTGGA+AGG 0.542146 2.4:+3421674 MS.gene85144:CDS
TGATTACAATAACACAGCAC+GGG 0.544650 2.4:-3423276 None:intergenic
TTTGCATCGTTTGTGGAGCA+GGG 0.545246 2.4:+3423230 MS.gene85144:CDS
GAACACAAAGTGTGTGTGAC+AGG 0.547699 2.4:+3421459 MS.gene85144:CDS
CCTTTGGGAATGGGAAAACC+AGG 0.548463 2.4:-3422678 None:intergenic
TTTGATATGTGATTGAGTAC+CGG 0.548510 2.4:-3421797 None:intergenic
TATGAAAACAAAGCCTCTCA+TGG 0.552465 2.4:+3422588 MS.gene85144:CDS
GCAAAACATTCAGCGTACCT+TGG 0.565410 2.4:-3422009 None:intergenic
GGCAAACTGAGAAATTTCAA+TGG 0.566240 2.4:+3422508 MS.gene85144:CDS
AGGCTAACACAACTCGAACA+AGG 0.571304 2.4:-3422054 None:intergenic
CCACATTATGAACTAAACAC+TGG 0.581273 2.4:+3423149 MS.gene85144:CDS
ATCTTGGAACCAGCGGTCCA+AGG 0.581499 2.4:+3421992 MS.gene85144:CDS
AACGCGATCATGGGATGCAA+AGG 0.582374 2.4:+3421755 MS.gene85144:CDS
GTTGGTGGGGAAGAGTGAGA+TGG 0.585295 2.4:-3423253 None:intergenic
GCAGTGGAACATAAATCTGT+TGG 0.588541 2.4:-3422371 None:intergenic
GCCTAACACATCAGATGCAG+TGG 0.588657 2.4:-3422387 None:intergenic
GATGTGTTAGGCTTGTTCAA+AGG 0.590361 2.4:+3422398 MS.gene85144:CDS
GGCTAACACAACTCGAACAA+GGG 0.595366 2.4:-3422053 None:intergenic
AGGATAACGATTTGCTAGCA+TGG 0.597463 2.4:-3422658 None:intergenic
TTGATTACAATAACACAGCA+CGG 0.605506 2.4:-3423277 None:intergenic
GGAATTGTGTGAGAAACTCC+AGG 0.606759 2.4:+3422153 MS.gene85144:CDS
GCAAAGGTATCTACCATGAG+AGG 0.637555 2.4:-3422601 None:intergenic
GGCAGAAATCTTGGAACCAG+CGG 0.644848 2.4:+3421985 MS.gene85144:intron
GTACCCAATGTCAAAGACAC+TGG 0.656150 2.4:+3422262 MS.gene85144:CDS
GATTACAATAACACAGCACG+GGG 0.703785 2.4:-3423275 None:intergenic
TCTAACAGCTGAAGACGATG+AGG 0.704563 2.4:-3422074 None:intergenic
TATGTGATTGAGTACCGGAG+AGG 0.740188 2.4:-3421792 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCATTTATGTTATTCAT+AGG + chr2.4:3421837-3421856 MS.gene85144:intron 15.0%
!! GATTAATTTCTTTATATCAA+TGG - chr2.4:3423029-3423048 None:intergenic 15.0%
!! GATTAATTTCTTTATATTGA+TGG - chr2.4:3422941-3422960 None:intergenic 15.0%
!! TATAAATATATGATTACTTG+AGG - chr2.4:3422188-3422207 None:intergenic 15.0%
!!! ATACTATAATGTTATTTGAT+TGG + chr2.4:3422888-3422907 MS.gene85144:intron 15.0%
!! AACAAACTTGTATTGAATTA+AGG + chr2.4:3422235-3422254 MS.gene85144:intron 20.0%
!! ATATCTTTGTATGATTGATT+TGG + chr2.4:3423100-3423119 MS.gene85144:intron 20.0%
!! ATATTGCTAATTAAACTAAC+AGG + chr2.4:3422486-3422505 MS.gene85144:intron 20.0%
!! ATGTAAACACAAAAAACTAA+TGG + chr2.4:3423075-3423094 MS.gene85144:intron 20.0%
!! ATTAATCACATCACAAATAT+TGG + chr2.4:3423042-3423061 MS.gene85144:intron 20.0%
!! TATTGCTAATTAAACTAACA+GGG + chr2.4:3422487-3422506 MS.gene85144:intron 20.0%
!! TGTAAACACAAAAAACTAAT+GGG + chr2.4:3423076-3423095 MS.gene85144:intron 20.0%
!!! AATAATGGATAATGATGATT+TGG + chr2.4:3422632-3422651 MS.gene85144:CDS 20.0%
!!! ATTCTATGATGTTATTTGAT+TGG + chr2.4:3422979-3422998 MS.gene85144:intron 20.0%
!!! TGTTAGTTTAATTAGCAATA+TGG - chr2.4:3422487-3422506 None:intergenic 20.0%
! TAAATGAAATATCACACCTT+AGG - chr2.4:3421827-3421846 None:intergenic 25.0%
! TAATCAAAATCTTCACATCA+TGG + chr2.4:3421893-3421912 MS.gene85144:intron 25.0%
! TATAGATACTAACTATGCTA+AGG - chr2.4:3422725-3422744 None:intergenic 25.0%
! TGATTAATGTATGAGCTTAT+AGG - chr2.4:3421879-3421898 None:intergenic 25.0%
!! CAATTTTTGTCCAAAAAAAC+AGG + chr2.4:3421964-3421983 MS.gene85144:intron 25.0%
!! GTTTTACCATCATTCATAAT+TGG + chr2.4:3422338-3422357 MS.gene85144:CDS 25.0%
!!! ACATTTTTTTATACACTTGC+AGG + chr2.4:3421635-3421654 MS.gene85144:intron 25.0%
AAAAAAACAGGCAGAAATCT+TGG + chr2.4:3421976-3421995 MS.gene85144:intron 30.0%
ACTTGTATTGAATTAAGGCA+TGG + chr2.4:3422240-3422259 MS.gene85144:intron 30.0%
AGCAGAAAGTTGAACATTTA+TGG + chr2.4:3421663-3421682 MS.gene85144:CDS 30.0%
AGTTGAACATTTATGGAAGT+TGG + chr2.4:3421670-3421689 MS.gene85144:CDS 30.0%
ATTGGAACAGTTAGAGATTT+AGG + chr2.4:3421543-3421562 MS.gene85144:CDS 30.0%
CCTTTGCAGTTCAAAAATAA+TGG + chr2.4:3422617-3422636 MS.gene85144:CDS 30.0%
TCACATATCAAATGATCCTA+AGG + chr2.4:3421808-3421827 MS.gene85144:CDS 30.0%
TTAGGTCCAATTATGAATGA+TGG - chr2.4:3422347-3422366 None:intergenic 30.0%
TTTCTTCCATTAAATCAGCT+TGG - chr2.4:3421724-3421743 None:intergenic 30.0%
TTTGATATGTGATTGAGTAC+CGG - chr2.4:3421800-3421819 None:intergenic 30.0%
! ATTAAGAGACTTCTTTTGTC+TGG + chr2.4:3421510-3421529 MS.gene85144:CDS 30.0%
! CATAAATCTGTTGGTAAGTT+AGG - chr2.4:3422365-3422384 None:intergenic 30.0%
!!! AAGATTTCTGCCTGTTTTTT+TGG - chr2.4:3421977-3421996 None:intergenic 30.0%
!!! AGGCTTTGTTTTCATAAAGA+AGG - chr2.4:3422584-3422603 None:intergenic 30.0%
!!! CCATTATTTTTGAACTGCAA+AGG - chr2.4:3422620-3422639 None:intergenic 30.0%
!!! GTATGATTGATTTGGTTTTC+AGG + chr2.4:3423108-3423127 MS.gene85144:intron 30.0%
ACTGAGAAATTTCAATGGCA+TGG + chr2.4:3422513-3422532 MS.gene85144:CDS 35.0%
AGAAATTTCAATGGCATGGA+AGG + chr2.4:3422517-3422536 MS.gene85144:CDS 35.0%
CAAAGAGAATGGAATAGACT+TGG + chr2.4:3422310-3422329 MS.gene85144:CDS 35.0%
CCACATTATGAACTAAACAC+TGG + chr2.4:3423149-3423168 MS.gene85144:CDS 35.0%
GAACATTTATGGAAGTTGGA+AGG + chr2.4:3421674-3421693 MS.gene85144:CDS 35.0%
GGCAAACTGAGAAATTTCAA+TGG + chr2.4:3422508-3422527 MS.gene85144:CDS 35.0%
TACATGCACACAAATGTTCT+AGG + chr2.4:3422421-3422440 MS.gene85144:intron 35.0%
TATGAAAACAAAGCCTCTCA+TGG + chr2.4:3422588-3422607 MS.gene85144:CDS 35.0%
TCAGGTTCAAGAAACTAGAT+AGG + chr2.4:3423126-3423145 MS.gene85144:intron 35.0%
TGGGAATATTGCAAAGAGAA+TGG + chr2.4:3422299-3422318 MS.gene85144:CDS 35.0%
TTGTCTGGATATCATGTCAT+TGG + chr2.4:3421525-3421544 MS.gene85144:CDS 35.0%
! ACTAGTCCAAGCTGATTTAA+TGG + chr2.4:3421715-3421734 MS.gene85144:CDS 35.0%
! CCAGTGTTTAGTTCATAATG+TGG - chr2.4:3423152-3423171 None:intergenic 35.0%
ATGCTAGCAAATCGTTATCC+TGG + chr2.4:3422660-3422679 MS.gene85144:CDS 40.0%
ATTTCAATGGCATGGAAGGA+TGG + chr2.4:3422521-3422540 MS.gene85144:CDS 40.0%
GCAGTGGAACATAAATCTGT+TGG - chr2.4:3422374-3422393 None:intergenic 40.0%
TACCATTAGACGAGTCATCT+TGG + chr2.4:3422122-3422141 MS.gene85144:CDS 40.0%
TACTTGAGGAAATGTGTACC+TGG - chr2.4:3422174-3422193 None:intergenic 40.0%
TGAACTAAACACTGGAAAGC+TGG + chr2.4:3423157-3423176 MS.gene85144:CDS 40.0%
TGATTGCTTTGCATCGTTTG+TGG + chr2.4:3423223-3423242 MS.gene85144:CDS 40.0%
TTTCAATGGCATGGAAGGAT+GGG + chr2.4:3422522-3422541 MS.gene85144:CDS 40.0%
! GATGTGTTAGGCTTGTTCAA+AGG + chr2.4:3422398-3422417 MS.gene85144:CDS 40.0%
!! AGGATAACGATTTGCTAGCA+TGG - chr2.4:3422661-3422680 None:intergenic 40.0%
ACTGGAAAGCTGGAGAGTTT+AGG + chr2.4:3423167-3423186 MS.gene85144:CDS 45.0%
ACTGGCAGAAAAAGCAGCTT+GGG + chr2.4:3422280-3422299 MS.gene85144:CDS 45.0%
AGGCTAACACAACTCGAACA+AGG - chr2.4:3422057-3422076 None:intergenic 45.0%
CTCCAAGATGACTCGTCTAA+TGG - chr2.4:3422127-3422146 None:intergenic 45.0%
CTGCCAGTGTCTTTGACATT+GGG - chr2.4:3422268-3422287 None:intergenic 45.0%
GAACACAAAGTGTGTGTGAC+AGG + chr2.4:3421459-3421478 MS.gene85144:CDS 45.0%
GAAGCATGAACCAACCCTTT+GGG - chr2.4:3422696-3422715 None:intergenic 45.0%
GCAAAACATTCAGCGTACCT+TGG - chr2.4:3422012-3422031 None:intergenic 45.0%
GGAATTGTGTGAGAAACTCC+AGG + chr2.4:3422153-3422172 MS.gene85144:CDS 45.0%
GGCTAACACAACTCGAACAA+GGG - chr2.4:3422056-3422075 None:intergenic 45.0%
GTACCCAATGTCAAAGACAC+TGG + chr2.4:3422262-3422281 MS.gene85144:CDS 45.0%
TCCACTGCATCTGATGTGTT+AGG + chr2.4:3422386-3422405 MS.gene85144:CDS 45.0%
TCTAACAGCTGAAGACGATG+AGG - chr2.4:3422077-3422096 None:intergenic 45.0%
TCTGCCAGTGTCTTTGACAT+TGG - chr2.4:3422269-3422288 None:intergenic 45.0%
TGTCTTTCACATTGCCTCTC+CGG + chr2.4:3421778-3421797 MS.gene85144:CDS 45.0%
TTTGCATCGTTTGTGGAGCA+GGG + chr2.4:3423230-3423249 MS.gene85144:CDS 45.0%
! AGAAGCATGAACCAACCCTT+TGG - chr2.4:3422697-3422716 None:intergenic 45.0%
! ATGAACCAACCCTTTGGGAA+TGG - chr2.4:3422691-3422710 None:intergenic 45.0%
! TGAACCAACCCTTTGGGAAT+GGG - chr2.4:3422690-3422709 None:intergenic 45.0%
! TTTTCCCATTCCCAAAGGGT+TGG + chr2.4:3422683-3422702 MS.gene85144:intron 45.0%
!! CTGGTTTTCCCATTCCCAAA+GGG + chr2.4:3422679-3422698 MS.gene85144:CDS 45.0%
!! GCAAAGGTATCTACCATGAG+AGG - chr2.4:3422604-3422623 None:intergenic 45.0%
!! TATGTGATTGAGTACCGGAG+AGG - chr2.4:3421795-3421814 None:intergenic 45.0%
!!! CTTCAGGCTTTTTGGCTTCT+TGG + chr2.4:3421484-3421503 MS.gene85144:CDS 45.0%
AACACAACTCGAACAAGGGC+TGG - chr2.4:3422052-3422071 None:intergenic 50.0%
AACGCGATCATGGGATGCAA+AGG + chr2.4:3421755-3421774 MS.gene85144:CDS 50.0%
ACAATAACACAGCACGGGGT+TGG - chr2.4:3423274-3423293 None:intergenic 50.0%
AGCTTCGACAACGCGATCAT+GGG + chr2.4:3421746-3421765 MS.gene85144:CDS 50.0%
CACTGGCAGAAAAAGCAGCT+TGG + chr2.4:3422279-3422298 MS.gene85144:CDS 50.0%
CGAGTCATCTTGGAGCTCAT+TGG + chr2.4:3422132-3422151 MS.gene85144:CDS 50.0%
CTTTGCATCGTTTGTGGAGC+AGG + chr2.4:3423229-3423248 MS.gene85144:CDS 50.0%
GCCTAACACATCAGATGCAG+TGG - chr2.4:3422390-3422409 None:intergenic 50.0%
GGCAGAAATCTTGGAACCAG+CGG + chr2.4:3421985-3422004 MS.gene85144:intron 50.0%
TAGCTTCGACAACGCGATCA+TGG + chr2.4:3421745-3421764 MS.gene85144:CDS 50.0%
! CCTTTGGGAATGGGAAAACC+AGG - chr2.4:3422681-3422700 None:intergenic 50.0%
! GACAGGAGCTTCAGGCTTTT+TGG + chr2.4:3421476-3421495 MS.gene85144:CDS 50.0%
!! CCTGGTTTTCCCATTCCCAA+AGG + chr2.4:3422678-3422697 MS.gene85144:CDS 50.0%
ATAACACAGCACGGGGTTGG+TGG - chr2.4:3423271-3423290 None:intergenic 55.0%
ATCTTGGAACCAGCGGTCCA+AGG + chr2.4:3421992-3422011 MS.gene85144:CDS 55.0%
GTGTGTGTGACAGGAGCTTC+AGG + chr2.4:3421468-3421487 MS.gene85144:CDS 55.0%
! GTTGGTGGGGAAGAGTGAGA+TGG - chr2.4:3423256-3423275 None:intergenic 55.0%
! TAACACAGCACGGGGTTGGT+GGG - chr2.4:3423270-3423289 None:intergenic 55.0%
TTCAGCGTACCTTGGACCGC+TGG - chr2.4:3422004-3422023 None:intergenic 60.0%
!! AACACAGCACGGGGTTGGTG+GGG - chr2.4:3423269-3423288 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3421456 3423295 3421456 ID=MS.gene85144
chr2.4 mRNA 3421456 3423295 3421456 ID=MS.gene85144.t1;Parent=MS.gene85144
chr2.4 exon 3421456 3421564 3421456 ID=MS.gene85144.t1.exon1;Parent=MS.gene85144.t1
chr2.4 CDS 3421456 3421564 3421456 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
chr2.4 exon 3421657 3421829 3421657 ID=MS.gene85144.t1.exon2;Parent=MS.gene85144.t1
chr2.4 CDS 3421657 3421829 3421657 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
chr2.4 exon 3421986 3422174 3421986 ID=MS.gene85144.t1.exon3;Parent=MS.gene85144.t1
chr2.4 CDS 3421986 3422174 3421986 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
chr2.4 exon 3422257 3422419 3422257 ID=MS.gene85144.t1.exon4;Parent=MS.gene85144.t1
chr2.4 CDS 3422257 3422419 3422257 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
chr2.4 exon 3422508 3422700 3422508 ID=MS.gene85144.t1.exon5;Parent=MS.gene85144.t1
chr2.4 CDS 3422508 3422700 3422508 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
chr2.4 exon 3423130 3423295 3423130 ID=MS.gene85144.t1.exon6;Parent=MS.gene85144.t1
chr2.4 CDS 3423130 3423295 3423130 ID=cds.MS.gene85144.t1;Parent=MS.gene85144.t1
Gene Sequence

>MS.gene85144

ATGGAACACAAAGTGTGTGTGACAGGAGCTTCAGGCTTTTTGGCTTCTTGGCTTATTAAGAGACTTCTTTTGTCTGGATATCATGTCATTGGAACAGTTAGAGATTTAGGTAAAATGTATTTTATGCTGACGAAATGTATTTTCACTTTTCACATTTAAATACATTTAACATATGTATGACATTTTTTTATACACTTGCAGGTAAGAAGCAGAAAGTTGAACATTTATGGAAGTTGGAAGGAGCAACAGAAAGACTCAAACTAGTCCAAGCTGATTTAATGGAAGAAAATAGCTTCGACAACGCGATCATGGGATGCAAAGGTGTCTTTCACATTGCCTCTCCGGTACTCAATCACATATCAAATGATCCTAAGGTGTGATATTTCATTTATGTTATTCATAGGTATCTCTATGTCACAACCTATAAGCTCATACATTAATCAAAATCTTCACATCATGGAAACTACACATAGTGAAATAAGTTATGATTAATTTCTAGTATGATTATCAATTTTTGTCCAAAAAAACAGGCAGAAATCTTGGAACCAGCGGTCCAAGGTACGCTGAATGTTTTGCGTTCTTGTCGAAAGAATCCAGCCCTTGTTCGAGTTGTGTTAGCCTCATCGTCTTCAGCTGTTAGAGTAAGAGCTGATTTCGATTCAAACATACCATTAGACGAGTCATCTTGGAGCTCATTGGAATTGTGTGAGAAACTCCAGGTACACATTTCCTCAAGTAATCATATATTTATAAGATTTTGATTAGCATGTACTTAATGTAACAAACTTGTATTGAATTAAGGCATGGTACCCAATGTCAAAGACACTGGCAGAAAAAGCAGCTTGGGAATATTGCAAAGAGAATGGAATAGACTTGGTGACTGTTTTACCATCATTCATAATTGGACCTAACTTACCAACAGATTTATGTTCCACTGCATCTGATGTGTTAGGCTTGTTCAAAGGTACATGCACACAAATGTTCTAGGCTTAATTTGATTGATATATTTTGTAAAATTATATGATTAACCATATTGCTAATTAAACTAACAGGGCAAACTGAGAAATTTCAATGGCATGGAAGGATGGGATATGTTCATATCGATGATGTTGCACTTTGTCACATCCTTCTTTATGAAAACAAAGCCTCTCATGGTAGATACCTTTGCAGTTCAAAAATAATGGATAATGATGATTTGGTTGCCATGCTAGCAAATCGTTATCCTGGTTTTCCCATTCCCAAAGGGTTGGTTCATGCTTCTCATTACCTTAGCATAGTTAGTATCTATATACATTTGATAGAGTATCGCAGATTTAATTTGTATACATAGACACTATAAAAATAAAATAAAATAAAATAAAATACACATGCATCAAATCAAATTAGATCGCGTAGATATTATTTAGAAATAAACTTAAGAAAATTGTACTTAATACTATAATGTTATTTGATTGGATACATGTGTAAAAAAAAAAAATTACACCATCAATATAAAGAAATTAATCACATTACAATTGTATTTAATTCTATGATGTTATTTGATTGGATACATGTGTAAAAAAAAATTACACCATTGATATAAAGAAATTAATCACATCACAAATATTGGAAACTAGAAAATGTAAACACAAAAAACTAATGGGTATATCTTTGTATGATTGATTTGGTTTTCAGGTTCAAGAAACTAGATAGGCCACATTATGAACTAAACACTGGAAAGCTGGAGAGTTTAGGATTCAAGTTTAAATCTGTTGAAGAAATGTTTGATGATTGCTTTGCATCGTTTGTGGAGCAGGGCCATCTCACTCTTCCCCACCAACCCCGTGCTGTGTTATTGTAA
Protein sequence

>MS.gene85144.t1

MEHKVCVTGASGFLASWLIKRLLLSGYHVIGTVRDLGKKQKVEHLWKLEGATERLKLVQADLMEENSFDNAIMGCKGVFHIASPVLNHISNDPKAEILEPAVQGTLNVLRSCRKNPALVRVVLASSSSAVRVRADFDSNIPLDESSWSSLELCEKLQAWYPMSKTLAEKAAWEYCKENGIDLVTVLPSFIIGPNLPTDLCSTASDVLGLFKGQTEKFQWHGRMGYVHIDDVALCHILLYENKASHGRYLCSSKIMDNDDLVAMLANRYPGFPIPKGFKKLDRPHYELNTGKLESLGFKFKSVEEMFDDCFASFVEQGHLTLPHQPRAVLL