AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85871

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85871.t1 MTR_5g042590 97.446 1018 19 4 1 1013 1 1016 0.0 2046
MS.gene85871.t1 MTR_6g477980 42.135 890 435 25 169 1013 261 1115 0.0 635
MS.gene85871.t1 MTR_6g477980 42.135 890 435 25 169 1013 255 1109 0.0 634
MS.gene85871.t1 MTR_1g102630 40.357 897 465 25 153 1013 116 978 0.0 612
MS.gene85871.t1 MTR_1g041345 40.223 895 467 25 152 1013 120 979 0.0 607
MS.gene85871.t1 MTR_4g056430 39.548 885 467 23 159 1013 125 971 0.0 602
MS.gene85871.t1 MTR_2g059590 39.201 926 493 25 122 1013 14 903 0.0 595
MS.gene85871.t1 MTR_2g028910 39.082 893 474 18 153 1012 173 1028 0.0 589
MS.gene85871.t1 MTR_4g056470 38.418 885 476 22 159 1013 163 1008 0.0 576
MS.gene85871.t1 MTR_4g083610 39.019 856 450 19 154 978 117 931 0.0 573
MS.gene85871.t1 MTR_4g113200 38.427 890 488 24 149 1013 22 876 0.0 569
MS.gene85871.t1 MTR_2g059790 42.454 603 305 13 433 1013 12 594 7.38e-151 461
MS.gene85871.t1 MTR_3g078647 31.853 901 522 29 159 1012 67 922 3.45e-124 401
MS.gene85871.t1 MTR_3g010650 31.579 931 526 34 135 1013 5 876 7.77e-122 394
MS.gene85871.t1 MTR_5g087870 29.494 929 550 29 139 1012 69 947 1.09e-114 376
MS.gene85871.t1 MTR_6g043180 29.957 928 540 29 138 1012 32 902 1.79e-112 369
MS.gene85871.t1 MTR_5g087890 29.859 921 539 33 138 1012 32 891 2.05e-111 366
MS.gene85871.t1 MTR_3g083300 31.366 915 532 28 151 1013 39 909 3.00e-111 366
MS.gene85871.t1 MTR_5g087870 29.053 919 545 30 139 1012 69 925 1.11e-108 360
MS.gene85871.t1 MTR_2g059590 34.443 691 405 21 122 792 14 676 1.42e-107 350
MS.gene85871.t1 MTR_1g038380 35.821 335 155 11 536 867 146 423 4.81e-41 157
MS.gene85871.t1 MTR_3g101730 41.860 172 68 4 740 911 33 172 8.18e-30 117
MS.gene85871.t1 MTR_6g037320 36.170 141 44 2 699 839 1 95 1.98e-18 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85871.t1 AT1G69440 70.790 873 217 7 149 1013 148 990 0.0 1283
MS.gene85871.t1 AT1G48410 39.194 1018 538 29 38 1013 70 1048 0.0 618
MS.gene85871.t1 AT1G48410 39.118 1020 538 30 38 1013 70 1050 0.0 613
MS.gene85871.t1 AT1G48410 39.118 1020 538 30 38 1013 70 1050 0.0 613
MS.gene85871.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene85871.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene85871.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene85871.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene85871.t1 AT2G27880 38.769 877 482 18 159 1013 154 997 0.0 586
MS.gene85871.t1 AT1G31280 36.805 845 473 19 153 976 163 967 1.98e-176 541
MS.gene85871.t1 AT1G31290 38.665 869 460 25 152 991 260 1084 3.75e-173 536
MS.gene85871.t1 AT1G31290 38.665 869 460 25 152 991 298 1122 9.39e-173 536
MS.gene85871.t1 AT1G31290 38.665 869 460 25 152 991 334 1158 2.91e-172 536
MS.gene85871.t1 AT5G21150 32.048 908 519 28 154 1013 39 896 9.26e-134 426
MS.gene85871.t1 AT2G27040 30.000 930 546 26 139 1013 45 924 2.66e-120 391
MS.gene85871.t1 AT2G27040 30.000 930 546 26 139 1013 45 924 2.66e-120 391
MS.gene85871.t1 AT5G21030 29.510 898 516 26 159 1013 31 854 9.39e-104 345
MS.gene85871.t1 AT2G32940 30.973 904 517 29 159 1013 33 878 4.10e-103 343
MS.gene85871.t1 AT5G21030 29.399 898 513 27 159 1013 31 850 3.04e-101 338

Find 197 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCCATCAACTTCTTTAA+AGG 0.105814 5.3:-29888254 None:intergenic
TCTTAACAATGTCACAATTT+TGG 0.115828 5.3:+29890076 MS.gene85871:CDS
AGAACTCAAGCTTATCATTT+TGG 0.173580 5.3:-29888558 None:intergenic
TTTCATATTGAAAGTGAAAA+TGG 0.210503 5.3:-29890796 None:intergenic
ACAGCTTCTTATTTGTATAA+TGG 0.263270 5.3:+29890142 MS.gene85871:CDS
ACAACTACAAAAGTAATAAA+AGG 0.290192 5.3:-29890713 None:intergenic
GTTCACATCTTTGAGTTAAC+TGG 0.291747 5.3:-29889992 None:intergenic
CGGAAGAAAATTATTCGGTT+TGG 0.299160 5.3:-29890596 None:intergenic
TTTCTATCTATGTAGTCATT+GGG 0.308144 5.3:+29890874 MS.gene85871:CDS
GTTAACTGGTTTATGAATCT+TGG 0.310605 5.3:-29889978 None:intergenic
AGGAGGCAACTGAAAATCTT+TGG 0.310887 5.3:+29889136 MS.gene85871:CDS
ATTTCTATCTATGTAGTCAT+TGG 0.316939 5.3:+29890873 MS.gene85871:CDS
GGAAGAAAATTATTCGGTTT+GGG 0.320501 5.3:-29890595 None:intergenic
GCAGGAGGAACTAATGAAAT+TGG 0.320784 5.3:-29891019 None:intergenic
TAGAGAAATCAAGCATAAAT+TGG 0.332646 5.3:+29888456 MS.gene85871:CDS
GTTTGCTTCTGAATTTCCTT+TGG 0.334202 5.3:-29888223 None:intergenic
TTCTCTAGCAACATCCTTAG+AGG 0.337003 5.3:-29888440 None:intergenic
AGATTCTTTCCATCTCTATC+AGG 0.345858 5.3:-29889162 None:intergenic
AATATCTTTGAAACTTGTTT+TGG 0.352913 5.3:-29887281 None:intergenic
CCATCACCAAGCTTTAGTTT+TGG 0.355041 5.3:-29889816 None:intergenic
TGTTGTTCACGTTTCTCATA+AGG 0.358507 5.3:-29888619 None:intergenic
ACAAATATATTTCAAGAATA+AGG 0.359133 5.3:+29890483 MS.gene85871:CDS
CTAGATGGCCATGTCTTTGA+AGG 0.360899 5.3:+29889891 MS.gene85871:CDS
GTCGATTTGCTAGTTGGAAT+TGG 0.361272 5.3:-29888592 None:intergenic
GGCGCTTTGCCGGCATATGA+TGG 0.365628 5.3:+29888505 MS.gene85871:CDS
AGGTGGGCACTAATAAGTTT+TGG 0.365715 5.3:+29889927 MS.gene85871:CDS
ATCTCTGAACGGTTTCTCTA+TGG 0.366520 5.3:-29889092 None:intergenic
TGCTCTCAATGTGGATTTCT+CGG 0.371590 5.3:+29888915 MS.gene85871:CDS
TTAACTGGTTTATGAATCTT+GGG 0.379326 5.3:-29889977 None:intergenic
CTTGAGAGATCAGAGTCCTT+AGG 0.384414 5.3:+29891082 MS.gene85871:CDS
GTTCAGAGATACCGTGTCTA+TGG 0.385771 5.3:+29889105 MS.gene85871:CDS
GCAGGATTATCTACATGCTT+TGG 0.385806 5.3:+29888741 MS.gene85871:CDS
GTTGCAAGGAGACCAGATTC+TGG 0.386600 5.3:+29888313 MS.gene85871:CDS
CCTTCTTGTCCACCAGAATC+TGG 0.394128 5.3:-29888325 None:intergenic
GAACTTAATTTGATGAGATT+CGG 0.394282 5.3:-29890245 None:intergenic
AATTACTCCTCATCCCTCTA+AGG 0.396696 5.3:+29888426 MS.gene85871:CDS
TGCATGGAAGTTTGTGCAAA+TGG 0.396886 5.3:-29887377 None:intergenic
GAAGGTAAAGGAAGAAGAGC+AGG 0.402774 5.3:-29887306 None:intergenic
GCATGTTCATCAAGGTTTCA+TGG 0.403651 5.3:+29890683 MS.gene85871:CDS
TGTAGAAACCAAGTTGAGTT+TGG 0.404992 5.3:-29887251 None:intergenic
TGCTTTGGATGTAGTCTTGA+GGG 0.405744 5.3:+29888756 MS.gene85871:CDS
CCATCTGTCGCAGCTGTTGT+TGG 0.405842 5.3:+29890437 MS.gene85871:CDS
ACTCAAAGATGTGAACAATT+AGG 0.405895 5.3:+29889999 MS.gene85871:CDS
GTCATTCTATTCGAGTTCAA+TGG 0.405912 5.3:+29888813 MS.gene85871:CDS
TCGATTTGCTAGTTGGAATT+GGG 0.409961 5.3:-29888591 None:intergenic
AGATAGAAATCAAATTCCTT+TGG 0.415078 5.3:-29890860 None:intergenic
CCATCCTTTAAAGAAGTTGA+TGG 0.415148 5.3:+29888250 MS.gene85871:CDS
TCATTCTATTCGAGTTCAAT+GGG 0.415940 5.3:+29888814 MS.gene85871:CDS
CAACAATACCAAACTCAACT+TGG 0.417364 5.3:+29887243 MS.gene85871:CDS
CGCCAATGGAACTTTCTAGA+TGG 0.421474 5.3:+29889876 MS.gene85871:CDS
ATAACTATGACATTCAATTC+AGG 0.422709 5.3:+29889214 MS.gene85871:CDS
TTGTGACATTGTTAAGAACT+TGG 0.423163 5.3:-29890070 None:intergenic
GGTGATGTCGATTTGCTAGT+TGG 0.436112 5.3:-29888598 None:intergenic
CGTCTCGGAAGAAAATTATT+CGG 0.437020 5.3:-29890601 None:intergenic
ACTGTTGTTGTTGCTGTTGA+GGG 0.440124 5.3:-29887200 None:intergenic
TTAGGTAGAGGTGGTGTCTT+CGG 0.441067 5.3:-29891145 None:intergenic
TACTGTTGTTGTTGCTGTTG+AGG 0.448198 5.3:-29887201 None:intergenic
TGTAGATAATCCTGCGGAAG+TGG 0.450340 5.3:-29888733 None:intergenic
ACTAAATACTTGAGCAAAGA+AGG 0.452270 5.3:+29888697 MS.gene85871:CDS
TGAGGAGCCGGTGATGTTCA+TGG 0.453148 5.3:+29890373 MS.gene85871:CDS
AAGAAGCTGTAGATTGTTTG+AGG 0.453692 5.3:-29890129 None:intergenic
AACAGTGCATCCACCAACTT+TGG 0.455056 5.3:-29890303 None:intergenic
GAGTTGTCTGCCTTTGAGAA+AGG 0.457326 5.3:-29889005 None:intergenic
TGTGAAGGACAGAAGTTCCT+TGG 0.458780 5.3:+29889303 MS.gene85871:CDS
TCTCATGACACCTTCGATGA+TGG 0.458848 5.3:-29889394 None:intergenic
CTCCTACTAATTTGCAAGCA+TGG 0.458921 5.3:-29889243 None:intergenic
GCACATTTAGCTGCATATAG+AGG 0.463633 5.3:+29891049 MS.gene85871:CDS
GAAAGATTGTATTCTGATGA+AGG 0.465705 5.3:-29887414 None:intergenic
GAAATATATTTGTTTGCTGT+TGG 0.469845 5.3:-29890473 None:intergenic
TCTCAACTTTCACTTGATCC+TGG 0.472145 5.3:+29887435 MS.gene85871:CDS
CTTTGGTTTCCTGATAGAGA+TGG 0.476622 5.3:+29889153 MS.gene85871:CDS
TCGAGGCGTTTCTGCAAATA+CGG 0.479257 5.3:-29888970 None:intergenic
CAGATCACACAAAGCTCCAT+AGG 0.479814 5.3:-29889282 None:intergenic
GAAGATTGATCGGTGTCAGC+AGG 0.480032 5.3:-29890764 None:intergenic
TACCCTCCAAAACTAAAGCT+TGG 0.483606 5.3:+29889810 MS.gene85871:CDS
CCATCAACTTCTTTAAAGGA+TGG 0.485273 5.3:-29888250 None:intergenic
TTTCGAAGATTGACGGGAAG+GGG 0.485680 5.3:+29888671 MS.gene85871:CDS
AACTAGTTTCGAAGATTGAC+GGG 0.490566 5.3:+29888665 MS.gene85871:CDS
AGAGAAATGACAAAGTTGAC+TGG 0.491533 5.3:+29889777 MS.gene85871:CDS
CTTGAATCGTCGAGAGGATG+AGG 0.492529 5.3:-29890413 None:intergenic
CAAGATGGGCTGTCAAAGAC+CGG 0.493342 5.3:+29889359 MS.gene85871:CDS
GGAGAAGTATTGAGTTTGCA+TGG 0.493972 5.3:-29887393 None:intergenic
CTCAGCTAACTTGTGAAGAA+AGG 0.494139 5.3:+29889025 MS.gene85871:CDS
GCATGGAAGTTTGTGCAAAT+GGG 0.494284 5.3:-29887376 None:intergenic
TAAAACAAGCATGTTCATCA+AGG 0.495580 5.3:+29890675 MS.gene85871:CDS
AGTTGTTGAAGTGAAGGTAA+AGG 0.498217 5.3:-29887318 None:intergenic
GAATAACTCCTATACTCTCA+TGG 0.500379 5.3:-29888948 None:intergenic
CAATATGAAAATATTCCACC+AGG 0.501529 5.3:+29890809 MS.gene85871:CDS
GAATAGAATGACCTTCCAAC+AGG 0.501556 5.3:-29888802 None:intergenic
CTGTTGTTGGTAGCATGAAC+TGG 0.502738 5.3:+29890450 MS.gene85871:CDS
GTTTCGAAGATTGACGGGAA+GGG 0.504847 5.3:+29888670 MS.gene85871:CDS
CTCAAGATCAATGCCAAAGT+TGG 0.507862 5.3:+29890290 MS.gene85871:CDS
GTGAAGGACAGAAGTTCCTT+GGG 0.508035 5.3:+29889304 MS.gene85871:CDS
GAGGAAATGTTGGCCCTACC+AGG 0.508892 5.3:+29889415 MS.gene85871:CDS
GCATTGCAGTTATACCTGGT+AGG 0.509794 5.3:-29889429 None:intergenic
ACTGCTTTCCATGAGAGTAT+AGG 0.509877 5.3:+29888940 MS.gene85871:CDS
TTGTCATTTCTCTTGAGACT+TGG 0.511045 5.3:-29889767 None:intergenic
TAAACCTTGTTATCTCCCTA+TGG 0.511898 5.3:+29889266 MS.gene85871:CDS
CATCCTTTAAAGAAGTTGAT+GGG 0.514420 5.3:+29888251 MS.gene85871:CDS
TGATAATGATTTAGCACCAA+AGG 0.516884 5.3:+29888207 MS.gene85871:intron
AAGATCAATGCCAAAGTTGG+TGG 0.519312 5.3:+29890293 MS.gene85871:CDS
AGAGCAAGTCCTTGTTGTGT+TGG 0.520077 5.3:-29888898 None:intergenic
GGCCATGCTTGCAAATTAGT+AGG 0.524814 5.3:+29889241 MS.gene85871:CDS
ATGTAGTCATTGGGGTGTGA+AGG 0.526206 5.3:+29890883 MS.gene85871:CDS
GCGCTTTGCCGGCATATGAT+GGG 0.527480 5.3:+29888506 MS.gene85871:CDS
GATATCGGTGGAGGAGCTGT+TGG 0.528041 5.3:+29888847 MS.gene85871:CDS
CTTGCAACAATTACTTCATG+TGG 0.531075 5.3:-29888298 None:intergenic
ACAGAATCAACCACAGTCCC+TGG 0.531566 5.3:-29890827 None:intergenic
TGAGGGAAAGATGGAATCAA+AGG 0.534413 5.3:-29887342 None:intergenic
GCAGATCTCGGTGCAATGGT+TGG 0.536050 5.3:+29890533 MS.gene85871:CDS
CCAACAACAGCTGCGACAGA+TGG 0.536587 5.3:-29890437 None:intergenic
ATGGGGAGAAGCAAAGATAT+CGG 0.538077 5.3:+29888832 MS.gene85871:CDS
CAAGAAATTATTGCAGATCT+CGG 0.541276 5.3:+29890521 MS.gene85871:CDS
AAAGGTAGTTGTTGAAGTGA+AGG 0.542969 5.3:-29887324 None:intergenic
GCTAAATGTGCATAATATGC+AGG 0.543695 5.3:-29891037 None:intergenic
GGAGGAGCTGTTGGATTGAG+AGG 0.544646 5.3:+29888856 MS.gene85871:CDS
GATTGTTTGAGGCAATACTC+TGG 0.544839 5.3:-29890118 None:intergenic
CGAAGGGATTCCACTTCCGC+AGG 0.547193 5.3:+29888723 MS.gene85871:CDS
GCTAACTTGTGAAGAAAGGA+AGG 0.550241 5.3:+29889029 MS.gene85871:CDS
TTGAAGGAACTACAATTGAA+AGG 0.550534 5.3:+29889907 MS.gene85871:CDS
GAGGAGCCGGTGATGTTCAT+GGG 0.550587 5.3:+29890374 MS.gene85871:CDS
CACAAAACATGGTAATGAGT+TGG 0.552639 5.3:-29890917 None:intergenic
CCAGATTCTGGTGGACAAGA+AGG 0.553420 5.3:+29888325 MS.gene85871:CDS
ACTGAGAAATGTATACCTGT+TGG 0.554582 5.3:+29888787 MS.gene85871:CDS
ATGACATTCAATTCAGGAAG+TGG 0.554927 5.3:+29889220 MS.gene85871:CDS
CTAAGGACTCTGATCTCTCA+AGG 0.557408 5.3:-29891081 None:intergenic
TATTGCAGATCTCGGTGCAA+TGG 0.560805 5.3:+29890529 MS.gene85871:CDS
CTTCGCGTCACGATCGCCAA+TGG 0.561844 5.3:+29889862 MS.gene85871:CDS
AAACTAGTTTCGAAGATTGA+CGG 0.563664 5.3:+29888664 MS.gene85871:CDS
CCAAAACTAAAGCTTGGTGA+TGG 0.566399 5.3:+29889816 MS.gene85871:CDS
TCACTAAGTTTAGGTAGAGG+TGG 0.567563 5.3:-29891154 None:intergenic
TGTAGTCATTGGGGTGTGAA+GGG 0.568049 5.3:+29890884 MS.gene85871:CDS
GGCCATCTAGAAAGTTCCAT+TGG 0.568195 5.3:-29889878 None:intergenic
CATTGCAGTTATACCTGGTA+GGG 0.568372 5.3:-29889428 None:intergenic
TTCTGTGATTACTCATCCAA+AGG 0.568582 5.3:+29890844 MS.gene85871:CDS
GAACGAAAAGCCATCATCGA+AGG 0.569372 5.3:+29889384 MS.gene85871:CDS
TCTCTAGCAACATCCTTAGA+GGG 0.573670 5.3:-29888439 None:intergenic
TTCTATCTATGTAGTCATTG+GGG 0.573801 5.3:+29890875 MS.gene85871:CDS
GAGAAATGACAAAGTTGACT+GGG 0.575964 5.3:+29889778 MS.gene85871:CDS
AAGGTAAAGGAAGAAGAGCA+GGG 0.582117 5.3:-29887305 None:intergenic
AGCACTTCTGAACAAACCTA+AGG 0.582641 5.3:-29891098 None:intergenic
ATTTGCTAGTTGGAATTGGG+AGG 0.582837 5.3:-29888588 None:intergenic
GCAACATCCTTAGAGGGATG+AGG 0.583299 5.3:-29888433 None:intergenic
AAGGAACTACAATTGAAAGG+TGG 0.584900 5.3:+29889910 MS.gene85871:CDS
GATTCGGATACAAGCAGCAC+TGG 0.585921 5.3:-29890229 None:intergenic
ATGCTTTGGATGTAGTCTTG+AGG 0.586671 5.3:+29888755 MS.gene85871:CDS
AAAAGACGCGGTAATTGAGA+AGG 0.586808 5.3:-29890344 None:intergenic
AGTTTCGAAGATTGACGGGA+AGG 0.591637 5.3:+29888669 MS.gene85871:CDS
AGGAACTACAATTGAAAGGT+GGG 0.592411 5.3:+29889911 MS.gene85871:CDS
AAACTAAAGCTTGGTGATGG+AGG 0.594557 5.3:+29889819 MS.gene85871:CDS
TTGTAGTTCCTTCAAAGACA+TGG 0.597249 5.3:-29889899 None:intergenic
AGAAGCAAAGATATCGGTGG+AGG 0.598026 5.3:+29888838 MS.gene85871:CDS
AGATCACACAAAGCTCCATA+GGG 0.598624 5.3:-29889281 None:intergenic
GGTGTCATGAGAGGAAATGT+TGG 0.600741 5.3:+29889405 MS.gene85871:CDS
CATTGGCGATCGTGACGCGA+AGG 0.601741 5.3:-29889861 None:intergenic
TTCTTAGAGACCTTTCTCAA+AGG 0.606629 5.3:+29888995 MS.gene85871:CDS
CACATGAAGTAATTGTTGCA+AGG 0.609332 5.3:+29888299 MS.gene85871:CDS
ATAGAGATGGAAAGAATCTG+AGG 0.612806 5.3:+29889166 MS.gene85871:CDS
TACAACACCGACACTTGTCT+CGG 0.616242 5.3:-29890204 None:intergenic
TCAGCTCCCATGAACATCAC+CGG 0.616771 5.3:-29890380 None:intergenic
TGAGCAAAGAAGGTGACGAA+GGG 0.621730 5.3:+29888707 MS.gene85871:CDS
GAAACTGTCGGATGATCAGA+CGG 0.621779 5.3:+29889326 MS.gene85871:CDS
AAATATTCCACCAGGGACTG+TGG 0.623325 5.3:+29890817 MS.gene85871:CDS
TTGAGCAAAGAAGGTGACGA+AGG 0.624886 5.3:+29888706 MS.gene85871:CDS
ATCATCGAAGGTGTCATGAG+AGG 0.629665 5.3:+29889396 MS.gene85871:CDS
AAGATGGGCTGTCAAAGACC+GGG 0.633687 5.3:+29889360 MS.gene85871:CDS
GAGTTTCGCTAACACCGTCT+CGG 0.634375 5.3:-29890616 None:intergenic
GAAGTTCCTTGGGAAACTGT+CGG 0.634410 5.3:+29889314 MS.gene85871:CDS
CGAATTGCCGAGACAAGTGT+CGG 0.636497 5.3:+29890197 MS.gene85871:CDS
ACTTGGATTGATTCAAACTG+CGG 0.638604 5.3:-29890053 None:intergenic
ACAGGTATACATTTCTCAGT+CGG 0.639287 5.3:-29888784 None:intergenic
GAGCTTTGTGTGATCTGTGA+AGG 0.639487 5.3:+29889288 MS.gene85871:CDS
AGAAATGTATACCTGTTGGA+AGG 0.640017 5.3:+29888791 MS.gene85871:CDS
AGCTCCATAGGGAGATAACA+AGG 0.643271 5.3:-29889270 None:intergenic
ACAAGGACTTGCTCTCAATG+TGG 0.644006 5.3:+29888906 MS.gene85871:CDS
AGTCTTAGACCAACACAACA+AGG 0.644925 5.3:+29888889 MS.gene85871:CDS
GGAAGAAGCTTATTTCAACA+AGG 0.658895 5.3:+29888272 MS.gene85871:CDS
CTTTGCCGGCATATGATGGG+AGG 0.659001 5.3:+29888509 MS.gene85871:CDS
TGTCTATGGCTTAACTGAGG+AGG 0.661469 5.3:+29889119 MS.gene85871:CDS
AAATGTGCATAATATGCAGG+AGG 0.662875 5.3:-29891034 None:intergenic
GGGAGAAGCAAAGATATCGG+TGG 0.666198 5.3:+29888835 MS.gene85871:CDS
GTTGTGCGTCGAAGATTGAT+CGG 0.667786 5.3:-29890774 None:intergenic
GCAAGGAGACCAGATTCTGG+TGG 0.670025 5.3:+29888316 MS.gene85871:CDS
AAAGGTCTCTAAGAAACTCG+AGG 0.671596 5.3:-29888987 None:intergenic
AATATGAAAATATTCCACCA+GGG 0.673069 5.3:+29890810 MS.gene85871:CDS
GATCATCCGACAGTTTCCCA+AGG 0.673171 5.3:-29889320 None:intergenic
GAATCAACCACAGTCCCTGG+TGG 0.680648 5.3:-29890824 None:intergenic
ATAGACACGGTATCTCTGAA+CGG 0.684701 5.3:-29889103 None:intergenic
TTGGCAAGGAGAGAGATCAC+AGG 0.686931 5.3:-29888349 None:intergenic
TCCTCAATATTGAAAAGACG+CGG 0.690933 5.3:-29890356 None:intergenic
GATGGACTTGAATCGTCGAG+AGG 0.693236 5.3:-29890419 None:intergenic
GGGGTGTGAAGGGAACAAGT+AGG 0.696897 5.3:+29890894 MS.gene85871:CDS
CATTCTATTCGAGTTCAATG+GGG 0.697995 5.3:+29888815 MS.gene85871:CDS
GAATAAGGTCTCAAACACAC+AGG 0.710521 5.3:+29890498 MS.gene85871:CDS
CCTCAGTTAAGCCATAGACA+CGG 0.718867 5.3:-29889116 None:intergenic
AAAGCATGTAGATAATCCTG+CGG 0.726216 5.3:-29888739 None:intergenic
CCGTGTCTATGGCTTAACTG+AGG 0.727424 5.3:+29889116 MS.gene85871:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCATAAATAAGA+CGG - chr5.3:29888198-29888217 None:intergenic 15.0%
!! ACAAATATATTTCAAGAATA+AGG + chr5.3:29890483-29890502 MS.gene85871:CDS 15.0%
!! CATCTATATTAAAAAAAACA+AGG - chr5.3:29887556-29887575 None:intergenic 15.0%
!!! TTAAATTTACTTTGACTTAT+TGG + chr5.3:29887627-29887646 MS.gene85871:intron 15.0%
!!! TTCATTCAAGTTAAAAAAAA+TGG - chr5.3:29887757-29887776 None:intergenic 15.0%
!! ACAACTACAAAAGTAATAAA+AGG - chr5.3:29890716-29890735 None:intergenic 20.0%
!! TTTCATATTGAAAGTGAAAA+TGG - chr5.3:29890799-29890818 None:intergenic 20.0%
!!! AATATCTTTGAAACTTGTTT+TGG - chr5.3:29887284-29887303 None:intergenic 20.0%
!!! ACTTTTTAAACATTGCATTT+GGG + chr5.3:29887698-29887717 MS.gene85871:intron 20.0%
!!! ATTTGTGTTTTACTTTTGTT+AGG + chr5.3:29890987-29891006 MS.gene85871:CDS 20.0%
!!! GTTTTTAATGTGAAAATGTA+AGG + chr5.3:29887812-29887831 MS.gene85871:intron 20.0%
!!! TACTTTTTAAACATTGCATT+TGG + chr5.3:29887697-29887716 MS.gene85871:intron 20.0%
!!! TCACTGTTCTTTTTTTTTTT+TGG + chr5.3:29887475-29887494 MS.gene85871:intron 20.0%
!!! TTATCATTTTGGAATTCAAT+TGG - chr5.3:29888550-29888569 None:intergenic 20.0%
! AAAAAAAGAACAGTGAAGAA+TGG - chr5.3:29887471-29887490 None:intergenic 25.0%
! AATATGAAAATATTCCACCA+GGG + chr5.3:29890810-29890829 MS.gene85871:CDS 25.0%
! AATCAAACTTCACCAAAAAA+TGG - chr5.3:29888375-29888394 None:intergenic 25.0%
! AGATAGAAATCAAATTCCTT+TGG - chr5.3:29890863-29890882 None:intergenic 25.0%
! ATAACTATGACATTCAATTC+AGG + chr5.3:29889214-29889233 MS.gene85871:CDS 25.0%
! ATTTCTATCTATGTAGTCAT+TGG + chr5.3:29890873-29890892 MS.gene85871:CDS 25.0%
! CAAAATCTCACAAACAAAAA+TGG + chr5.3:29887138-29887157 MS.gene85871:CDS 25.0%
! CTTAAAAAACCAAACAAACA+AGG - chr5.3:29887599-29887618 None:intergenic 25.0%
! CTTATGTGAAGAATGTTATT+AGG - chr5.3:29888083-29888102 None:intergenic 25.0%
! GTCATGTTTCAAAAACATAA+AGG - chr5.3:29887864-29887883 None:intergenic 25.0%
! TAATGGAGAAAAAACACAAA+GGG + chr5.3:29890159-29890178 MS.gene85871:CDS 25.0%
! TAGAGAAATCAAGCATAAAT+TGG + chr5.3:29888456-29888475 MS.gene85871:CDS 25.0%
! TTTCTATCTATGTAGTCATT+GGG + chr5.3:29890874-29890893 MS.gene85871:CDS 25.0%
!! ACAGCTTCTTATTTGTATAA+TGG + chr5.3:29890142-29890161 MS.gene85871:CDS 25.0%
!! ATAATGGAGAAAAAACACAA+AGG + chr5.3:29890158-29890177 MS.gene85871:CDS 25.0%
!! CAAATTAAGTTCACAGTTTT+TGG + chr5.3:29890256-29890275 MS.gene85871:CDS 25.0%
!! CTTTTTAAACATTGCATTTG+GGG + chr5.3:29887699-29887718 MS.gene85871:intron 25.0%
!! GAACTTAATTTGATGAGATT+CGG - chr5.3:29890248-29890267 None:intergenic 25.0%
!! GTGAACAACATAAACTTTTT+CGG + chr5.3:29888632-29888651 MS.gene85871:CDS 25.0%
!! GTTTGATTCAGATTTTACAT+TGG + chr5.3:29887782-29887801 MS.gene85871:intron 25.0%
!! TAACAATGCTGAGATTTTAT+CGG + chr5.3:29888483-29888502 MS.gene85871:CDS 25.0%
!! TCTTAACAATGTCACAATTT+TGG + chr5.3:29890076-29890095 MS.gene85871:CDS 25.0%
!! TGAAGATTTTTATCAAGAAG+TGG + chr5.3:29890565-29890584 MS.gene85871:CDS 25.0%
!! TTAACTGGTTTATGAATCTT+GGG - chr5.3:29889980-29889999 None:intergenic 25.0%
!! TTGATGTTTTCACTAAGTTT+AGG - chr5.3:29891166-29891185 None:intergenic 25.0%
!!! ATTTTGGTTAGTGTTTTTGT+TGG - chr5.3:29887124-29887143 None:intergenic 25.0%
!!! CATTTTTGTTTGTGAGATTT+TGG - chr5.3:29887140-29887159 None:intergenic 25.0%
!!! GAAATATATTTGTTTGCTGT+TGG - chr5.3:29890476-29890495 None:intergenic 25.0%
!!! TTTTTAACTTGAATGAAGCT+AGG + chr5.3:29887760-29887779 MS.gene85871:intron 25.0%
AAACTTCACCAAAAAATGGT+TGG - chr5.3:29888371-29888390 None:intergenic 30.0%
AAGTGAAATGATCACTTGTT+TGG + chr5.3:29887963-29887982 MS.gene85871:intron 30.0%
ACTAAATACTTGAGCAAAGA+AGG + chr5.3:29888697-29888716 MS.gene85871:CDS 30.0%
ACTCAAAGATGTGAACAATT+AGG + chr5.3:29889999-29890018 MS.gene85871:CDS 30.0%
ATGAAAAGAACAGTGAAGAA+TGG - chr5.3:29887507-29887526 None:intergenic 30.0%
CAAGAAATTATTGCAGATCT+CGG + chr5.3:29890521-29890540 MS.gene85871:CDS 30.0%
CAATATGAAAATATTCCACC+AGG + chr5.3:29890809-29890828 MS.gene85871:CDS 30.0%
CCACGTAATCACTTAATAAA+CGG - chr5.3:29889612-29889631 None:intergenic 30.0%
GAAAGATTGTATTCTGATGA+AGG - chr5.3:29887417-29887436 None:intergenic 30.0%
GGAAGAAAATTATTCGGTTT+GGG - chr5.3:29890598-29890617 None:intergenic 30.0%
TAAAACAAGCATGTTCATCA+AGG + chr5.3:29890675-29890694 MS.gene85871:CDS 30.0%
TCACTTAATAAACGGTTTCA+CGG - chr5.3:29889604-29889623 None:intergenic 30.0%
TTATTAAGTGATTACGTGGA+CGG + chr5.3:29889613-29889632 MS.gene85871:intron 30.0%
TTCTATCTATGTAGTCATTG+GGG + chr5.3:29890875-29890894 MS.gene85871:CDS 30.0%
TTGAAGGAACTACAATTGAA+AGG + chr5.3:29889907-29889926 MS.gene85871:CDS 30.0%
TTGTGACATTGTTAAGAACT+TGG - chr5.3:29890073-29890092 None:intergenic 30.0%
! AAAAGTAATCCTTTTGACAG+TGG + chr5.3:29889720-29889739 MS.gene85871:intron 30.0%
! AAACTAGTTTCGAAGATTGA+CGG + chr5.3:29888664-29888683 MS.gene85871:CDS 30.0%
! AACAATGCTGAGATTTTATC+GGG + chr5.3:29888484-29888503 MS.gene85871:CDS 30.0%
! AACTCATTACCATGTTTTGT+GGG + chr5.3:29890919-29890938 MS.gene85871:CDS 30.0%
! AGAACTCAAGCTTATCATTT+TGG - chr5.3:29888561-29888580 None:intergenic 30.0%
! CATCCTTTAAAGAAGTTGAT+GGG + chr5.3:29888251-29888270 MS.gene85871:CDS 30.0%
! CTTTTGTAGTTGTGCAAAAA+AGG + chr5.3:29890723-29890742 MS.gene85871:CDS 30.0%
! GTTAACTGGTTTATGAATCT+TGG - chr5.3:29889981-29890000 None:intergenic 30.0%
! TATTTTCATCCCACAAAACA+TGG - chr5.3:29890931-29890950 None:intergenic 30.0%
! TCATTCTATTCGAGTTCAAT+GGG + chr5.3:29888814-29888833 MS.gene85871:CDS 30.0%
! TGATAATGATTTAGCACCAA+AGG + chr5.3:29888207-29888226 MS.gene85871:intron 30.0%
! TTTTCACTAAGTTTAGGTAG+AGG - chr5.3:29891160-29891179 None:intergenic 30.0%
!! TTCTTTAAGCCTTGTTTGTT+TGG + chr5.3:29887587-29887606 MS.gene85871:intron 30.0%
!!! AAATGGGTTTTGAGATTTTG+AGG - chr5.3:29887363-29887382 None:intergenic 30.0%
!!! AATGGGTTTTGAGATTTTGA+GGG - chr5.3:29887362-29887381 None:intergenic 30.0%
!!! TTCACAGTTTTTGGCTAATT+TGG + chr5.3:29890265-29890284 MS.gene85871:CDS 30.0%
AAACAAGGAATGCAAGCAAA+AGG - chr5.3:29887541-29887560 None:intergenic 35.0%
AAAGCATGTAGATAATCCTG+CGG - chr5.3:29888742-29888761 None:intergenic 35.0%
AAAGGTAGTTGTTGAAGTGA+AGG - chr5.3:29887327-29887346 None:intergenic 35.0%
AAATGTGCATAATATGCAGG+AGG - chr5.3:29891037-29891056 None:intergenic 35.0%
AACAAGGAATGCAAGCAAAA+GGG - chr5.3:29887540-29887559 None:intergenic 35.0%
AAGAAGCTGTAGATTGTTTG+AGG - chr5.3:29890132-29890151 None:intergenic 35.0%
AAGGAACTACAATTGAAAGG+TGG + chr5.3:29889910-29889929 MS.gene85871:CDS 35.0%
ACAGGTATACATTTCTCAGT+CGG - chr5.3:29888787-29888806 None:intergenic 35.0%
ACGGCAAAAATACTCAAGAT+GGG + chr5.3:29889345-29889364 MS.gene85871:CDS 35.0%
ACTGAGAAATGTATACCTGT+TGG + chr5.3:29888787-29888806 MS.gene85871:CDS 35.0%
AGAAATGTATACCTGTTGGA+AGG + chr5.3:29888791-29888810 MS.gene85871:CDS 35.0%
AGAGAAATGACAAAGTTGAC+TGG + chr5.3:29889777-29889796 MS.gene85871:CDS 35.0%
AGATTCTTTCCATCTCTATC+AGG - chr5.3:29889165-29889184 None:intergenic 35.0%
AGGAACTACAATTGAAAGGT+GGG + chr5.3:29889911-29889930 MS.gene85871:CDS 35.0%
AGTTGTTGAAGTGAAGGTAA+AGG - chr5.3:29887321-29887340 None:intergenic 35.0%
ATAGAGATGGAAAGAATCTG+AGG + chr5.3:29889166-29889185 MS.gene85871:CDS 35.0%
ATGACATTCAATTCAGGAAG+TGG + chr5.3:29889220-29889239 MS.gene85871:CDS 35.0%
CAACAATACCAAACTCAACT+TGG + chr5.3:29887243-29887262 MS.gene85871:CDS 35.0%
CACATGAAGTAATTGTTGCA+AGG + chr5.3:29888299-29888318 MS.gene85871:CDS 35.0%
CCGTTTATTAAGTGATTACG+TGG + chr5.3:29889609-29889628 MS.gene85871:intron 35.0%
CGGAAGAAAATTATTCGGTT+TGG - chr5.3:29890599-29890618 None:intergenic 35.0%
CGTCTCGGAAGAAAATTATT+CGG - chr5.3:29890604-29890623 None:intergenic 35.0%
CTTCCCATCAACTTCTTTAA+AGG - chr5.3:29888257-29888276 None:intergenic 35.0%
CTTGCAACAATTACTTCATG+TGG - chr5.3:29888301-29888320 None:intergenic 35.0%
GAATAACTCCTATACTCTCA+TGG - chr5.3:29888951-29888970 None:intergenic 35.0%
GAGAAATGACAAAGTTGACT+GGG + chr5.3:29889778-29889797 MS.gene85871:CDS 35.0%
GCTAAATGTGCATAATATGC+AGG - chr5.3:29891040-29891059 None:intergenic 35.0%
GGAAGAAGCTTATTTCAACA+AGG + chr5.3:29888272-29888291 MS.gene85871:CDS 35.0%
GTTTGCTTCTGAATTTCCTT+TGG - chr5.3:29888226-29888245 None:intergenic 35.0%
TCCTCAATATTGAAAAGACG+CGG - chr5.3:29890359-29890378 None:intergenic 35.0%
TGTTGTTCACGTTTCTCATA+AGG - chr5.3:29888622-29888641 None:intergenic 35.0%
TTCTGTGATTACTCATCCAA+AGG + chr5.3:29890844-29890863 MS.gene85871:CDS 35.0%
TTCTTAGAGACCTTTCTCAA+AGG + chr5.3:29888995-29889014 MS.gene85871:CDS 35.0%
TTGTAGTTCCTTCAAAGACA+TGG - chr5.3:29889902-29889921 None:intergenic 35.0%
! AACTAGTTTCGAAGATTGAC+GGG + chr5.3:29888665-29888684 MS.gene85871:CDS 35.0%
! ACTTGGATTGATTCAAACTG+CGG - chr5.3:29890056-29890075 None:intergenic 35.0%
! CAACTCATTACCATGTTTTG+TGG + chr5.3:29890918-29890937 MS.gene85871:CDS 35.0%
! CACAAAACATGGTAATGAGT+TGG - chr5.3:29890920-29890939 None:intergenic 35.0%
! CATTCTATTCGAGTTCAATG+GGG + chr5.3:29888815-29888834 MS.gene85871:CDS 35.0%
! CCATCAACTTCTTTAAAGGA+TGG - chr5.3:29888253-29888272 None:intergenic 35.0%
! CCATCCTTTAAAGAAGTTGA+TGG + chr5.3:29888250-29888269 MS.gene85871:CDS 35.0%
! CGAATAATTTTCTTCCGAGA+CGG + chr5.3:29890602-29890621 MS.gene85871:CDS 35.0%
! GGAGTTTGAATTCATTTTCC+TGG - chr5.3:29889749-29889768 None:intergenic 35.0%
! GTCATTCTATTCGAGTTCAA+TGG + chr5.3:29888813-29888832 MS.gene85871:CDS 35.0%
! GTTCACATCTTTGAGTTAAC+TGG - chr5.3:29889995-29890014 None:intergenic 35.0%
! GTTTTACAAAGTTCTGCAAG+AGG + chr5.3:29890640-29890659 MS.gene85871:CDS 35.0%
! TAAACCTTGTTATCTCCCTA+TGG + chr5.3:29889266-29889285 MS.gene85871:CDS 35.0%
! TCTTTTCAATATTGAGGAGC+CGG + chr5.3:29890361-29890380 MS.gene85871:CDS 35.0%
! TGTAGAAACCAAGTTGAGTT+TGG - chr5.3:29887254-29887273 None:intergenic 35.0%
! TTGTCATTTCTCTTGAGACT+TGG - chr5.3:29889770-29889789 None:intergenic 35.0%
!! TCGATTTGCTAGTTGGAATT+GGG - chr5.3:29888594-29888613 None:intergenic 35.0%
!! TTGAGATTTTGAGGGAAAGA+TGG - chr5.3:29887354-29887373 None:intergenic 35.0%
AAAAGACGCGGTAATTGAGA+AGG - chr5.3:29890347-29890366 None:intergenic 40.0%
AAGAATGGTGCTTACTTACC+AGG - chr5.3:29887456-29887475 None:intergenic 40.0%
AAGATCAATGCCAAAGTTGG+TGG + chr5.3:29890293-29890312 MS.gene85871:CDS 40.0%
AAGGTAAAGGAAGAAGAGCA+GGG - chr5.3:29887308-29887327 None:intergenic 40.0%
AATTACTCCTCATCCCTCTA+AGG + chr5.3:29888426-29888445 MS.gene85871:CDS 40.0%
AGATCACACAAAGCTCCATA+GGG - chr5.3:29889284-29889303 None:intergenic 40.0%
AGGAGGCAACTGAAAATCTT+TGG + chr5.3:29889136-29889155 MS.gene85871:CDS 40.0%
AGTCTTAGACCAACACAACA+AGG + chr5.3:29888889-29888908 MS.gene85871:CDS 40.0%
ATAGACACGGTATCTCTGAA+CGG - chr5.3:29889106-29889125 None:intergenic 40.0%
ATCTCTGAACGGTTTCTCTA+TGG - chr5.3:29889095-29889114 None:intergenic 40.0%
ATGGGGAGAAGCAAAGATAT+CGG + chr5.3:29888832-29888851 MS.gene85871:CDS 40.0%
CATTGCAGTTATACCTGGTA+GGG - chr5.3:29889431-29889450 None:intergenic 40.0%
CCTCATAAAAACCAGAACAC+TGG + chr5.3:29889550-29889569 MS.gene85871:intron 40.0%
CTCAAGATCAATGCCAAAGT+TGG + chr5.3:29890290-29890309 MS.gene85871:CDS 40.0%
GAATAAGGTCTCAAACACAC+AGG + chr5.3:29890498-29890517 MS.gene85871:CDS 40.0%
GAATAGAATGACCTTCCAAC+AGG - chr5.3:29888805-29888824 None:intergenic 40.0%
GACGGCAAAAATACTCAAGA+TGG + chr5.3:29889344-29889363 MS.gene85871:CDS 40.0%
GCACATTTAGCTGCATATAG+AGG + chr5.3:29891049-29891068 MS.gene85871:CDS 40.0%
GCAGGAGGAACTAATGAAAT+TGG - chr5.3:29891022-29891041 None:intergenic 40.0%
GCAGGATTATCTACATGCTT+TGG + chr5.3:29888741-29888760 MS.gene85871:CDS 40.0%
GCATGGAAGTTTGTGCAAAT+GGG - chr5.3:29887379-29887398 None:intergenic 40.0%
GCATGTTCATCAAGGTTTCA+TGG + chr5.3:29890683-29890702 MS.gene85871:CDS 40.0%
GTTAAGATACTCCAGTGTTC+TGG - chr5.3:29889564-29889583 None:intergenic 40.0%
TACCCTCCAAAACTAAAGCT+TGG + chr5.3:29889810-29889829 MS.gene85871:CDS 40.0%
TCACCAAAAAATGGTTGGCA+AGG - chr5.3:29888366-29888385 None:intergenic 40.0%
TCACTAAGTTTAGGTAGAGG+TGG - chr5.3:29891157-29891176 None:intergenic 40.0%
TCTCAACTTTCACTTGATCC+TGG + chr5.3:29887435-29887454 MS.gene85871:CDS 40.0%
TCTCTAGCAACATCCTTAGA+GGG - chr5.3:29888442-29888461 None:intergenic 40.0%
TGAGGGAAAGATGGAATCAA+AGG - chr5.3:29887345-29887364 None:intergenic 40.0%
TGATTACGTGGACGGTTAAA+TGG + chr5.3:29889621-29889640 MS.gene85871:intron 40.0%
TGCATGGAAGTTTGTGCAAA+TGG - chr5.3:29887380-29887399 None:intergenic 40.0%
TGCTCTCAATGTGGATTTCT+CGG + chr5.3:29888915-29888934 MS.gene85871:CDS 40.0%
TTCTCTAGCAACATCCTTAG+AGG - chr5.3:29888443-29888462 None:intergenic 40.0%
! AAAGGTCTCTAAGAAACTCG+AGG - chr5.3:29888990-29889009 None:intergenic 40.0%
! ACCGCGTCTTTTCAATATTG+AGG + chr5.3:29890355-29890374 MS.gene85871:CDS 40.0%
! ACTGCTTTCCATGAGAGTAT+AGG + chr5.3:29888940-29888959 MS.gene85871:CDS 40.0%
! ACTGTTGTTGTTGCTGTTGA+GGG - chr5.3:29887203-29887222 None:intergenic 40.0%
! AGGTGGGCACTAATAAGTTT+TGG + chr5.3:29889927-29889946 MS.gene85871:CDS 40.0%
! ATGCTTTGGATGTAGTCTTG+AGG + chr5.3:29888755-29888774 MS.gene85871:CDS 40.0%
! ATTTGCTAGTTGGAATTGGG+AGG - chr5.3:29888591-29888610 None:intergenic 40.0%
! CCAAAACTAAAGCTTGGTGA+TGG + chr5.3:29889816-29889835 MS.gene85871:CDS 40.0%
! CTCAGCTAACTTGTGAAGAA+AGG + chr5.3:29889025-29889044 MS.gene85871:CDS 40.0%
! CTCCTACTAATTTGCAAGCA+TGG - chr5.3:29889246-29889265 None:intergenic 40.0%
! CTTTGGTTTCCTGATAGAGA+TGG + chr5.3:29889153-29889172 MS.gene85871:CDS 40.0%
! GATTGTTTGAGGCAATACTC+TGG - chr5.3:29890121-29890140 None:intergenic 40.0%
! GCTAACTTGTGAAGAAAGGA+AGG + chr5.3:29889029-29889048 MS.gene85871:CDS 40.0%
! TACTGTTGTTGTTGCTGTTG+AGG - chr5.3:29887204-29887223 None:intergenic 40.0%
! TGCTTTGGATGTAGTCTTGA+GGG + chr5.3:29888756-29888775 MS.gene85871:CDS 40.0%
! TTTTTCCTCCCATCATATGC+CGG - chr5.3:29888517-29888536 None:intergenic 40.0%
!! AAACTAAAGCTTGGTGATGG+AGG + chr5.3:29889819-29889838 MS.gene85871:CDS 40.0%
!! CCATCACCAAGCTTTAGTTT+TGG - chr5.3:29889819-29889838 None:intergenic 40.0%
!! GGAGAAGTATTGAGTTTGCA+TGG - chr5.3:29887396-29887415 None:intergenic 40.0%
!! GTCGATTTGCTAGTTGGAAT+TGG - chr5.3:29888595-29888614 None:intergenic 40.0%
!! TGCAAAAAAGGCATCACACA+AGG + chr5.3:29890735-29890754 MS.gene85871:CDS 40.0%
!! TGGGCACTAATAAGTTTTGG+AGG + chr5.3:29889930-29889949 MS.gene85871:CDS 40.0%
!!! CACCAAGCTTTAGTTTTGGA+GGG - chr5.3:29889815-29889834 None:intergenic 40.0%
!!! CCAGTGTTCTGGTTTTTATG+AGG - chr5.3:29889553-29889572 None:intergenic 40.0%
!!! TCACCAAGCTTTAGTTTTGG+AGG - chr5.3:29889816-29889835 None:intergenic 40.0%
!!! TCTCCTTGCCAACCATTTTT+TGG + chr5.3:29888360-29888379 MS.gene85871:CDS 40.0%
AAATATTCCACCAGGGACTG+TGG + chr5.3:29890817-29890836 MS.gene85871:CDS 45.0%
AACAGTGCATCCACCAACTT+TGG - chr5.3:29890306-29890325 None:intergenic 45.0%
ACGGTTTCACGGTTTGTGAA+TGG - chr5.3:29889593-29889612 None:intergenic 45.0%
AGAAGCAAAGATATCGGTGG+AGG + chr5.3:29888838-29888857 MS.gene85871:CDS 45.0%
AGCTCCATAGGGAGATAACA+AGG - chr5.3:29889273-29889292 None:intergenic 45.0%
AGTTTCGAAGATTGACGGGA+AGG + chr5.3:29888669-29888688 MS.gene85871:CDS 45.0%
ATGTAGTCATTGGGGTGTGA+AGG + chr5.3:29890883-29890902 MS.gene85871:CDS 45.0%
CACGGTTTGTGAATGGATGT+TGG - chr5.3:29889586-29889605 None:intergenic 45.0%
CAGATCACACAAAGCTCCAT+AGG - chr5.3:29889285-29889304 None:intergenic 45.0%
CCTCAGTTAAGCCATAGACA+CGG - chr5.3:29889119-29889138 None:intergenic 45.0%
CGCCAATGGAACTTTCTAGA+TGG + chr5.3:29889876-29889895 MS.gene85871:CDS 45.0%
CTAGATGGCCATGTCTTTGA+AGG + chr5.3:29889891-29889910 MS.gene85871:CDS 45.0%
GAAACTGTCGGATGATCAGA+CGG + chr5.3:29889326-29889345 MS.gene85871:CDS 45.0%
GAACGAAAAGCCATCATCGA+AGG + chr5.3:29889384-29889403 MS.gene85871:CDS 45.0%
GAAGGTAAAGGAAGAAGAGC+AGG - chr5.3:29887309-29887328 None:intergenic 45.0%
GAAGTTCCTTGGGAAACTGT+CGG + chr5.3:29889314-29889333 MS.gene85871:CDS 45.0%
GCATTGCAGTTATACCTGGT+AGG - chr5.3:29889432-29889451 None:intergenic 45.0%
GGCCATCTAGAAAGTTCCAT+TGG - chr5.3:29889881-29889900 None:intergenic 45.0%
GGCCATGCTTGCAAATTAGT+AGG + chr5.3:29889241-29889260 MS.gene85871:CDS 45.0%
GTGAAGGACAGAAGTTCCTT+GGG + chr5.3:29889304-29889323 MS.gene85871:CDS 45.0%
GTTCAGAGATACCGTGTCTA+TGG + chr5.3:29889105-29889124 MS.gene85871:CDS 45.0%
GTTGTGCGTCGAAGATTGAT+CGG - chr5.3:29890777-29890796 None:intergenic 45.0%
GTTTCGAAGATTGACGGGAA+GGG + chr5.3:29888670-29888689 MS.gene85871:CDS 45.0%
TACAACACCGACACTTGTCT+CGG - chr5.3:29890207-29890226 None:intergenic 45.0%
TCCTGGTTACCACTGTCAAA+AGG - chr5.3:29889732-29889751 None:intergenic 45.0%
TCTCATGACACCTTCGATGA+TGG - chr5.3:29889397-29889416 None:intergenic 45.0%
TGAGCAAAGAAGGTGACGAA+GGG + chr5.3:29888707-29888726 MS.gene85871:CDS 45.0%
TGCTGCATTGCAGTTATACC+TGG - chr5.3:29889436-29889455 None:intergenic 45.0%
TGTAGATAATCCTGCGGAAG+TGG - chr5.3:29888736-29888755 None:intergenic 45.0%
TGTAGTCATTGGGGTGTGAA+GGG + chr5.3:29890884-29890903 MS.gene85871:CDS 45.0%
TGTCTATGGCTTAACTGAGG+AGG + chr5.3:29889119-29889138 MS.gene85871:CDS 45.0%
TGTGAAGGACAGAAGTTCCT+TGG + chr5.3:29889303-29889322 MS.gene85871:CDS 45.0%
TTAGGTAGAGGTGGTGTCTT+CGG - chr5.3:29891148-29891167 None:intergenic 45.0%
TTGAGCAAAGAAGGTGACGA+AGG + chr5.3:29888706-29888725 MS.gene85871:CDS 45.0%
TTTCGAAGATTGACGGGAAG+GGG + chr5.3:29888671-29888690 MS.gene85871:CDS 45.0%
! ACAAGGACTTGCTCTCAATG+TGG + chr5.3:29888906-29888925 MS.gene85871:CDS 45.0%
! AGAGCAAGTCCTTGTTGTGT+TGG - chr5.3:29888901-29888920 None:intergenic 45.0%
! ATGATGGCTTTTCGTTCTCC+CGG - chr5.3:29889381-29889400 None:intergenic 45.0%
! GAGCTTTGTGTGATCTGTGA+AGG + chr5.3:29889288-29889307 MS.gene85871:CDS 45.0%
! GAGTTGTCTGCCTTTGAGAA+AGG - chr5.3:29889008-29889027 None:intergenic 45.0%
! GGGATATGAGACTTTTGCTC+AGG - chr5.3:29889960-29889979 None:intergenic 45.0%
! GGTGTCATGAGAGGAAATGT+TGG + chr5.3:29889405-29889424 MS.gene85871:CDS 45.0%
! TATTGCAGATCTCGGTGCAA+TGG + chr5.3:29890529-29890548 MS.gene85871:CDS 45.0%
! TCCTTTTGACAGTGGTAACC+AGG + chr5.3:29889728-29889747 MS.gene85871:intron 45.0%
! TCGAGGCGTTTCTGCAAATA+CGG - chr5.3:29888973-29888992 None:intergenic 45.0%
!! ATCATCGAAGGTGTCATGAG+AGG + chr5.3:29889396-29889415 MS.gene85871:CDS 45.0%
!! CTGTTGTTGGTAGCATGAAC+TGG + chr5.3:29890450-29890469 MS.gene85871:CDS 45.0%
!! GGTGATGTCGATTTGCTAGT+TGG - chr5.3:29888601-29888620 None:intergenic 45.0%
!!! TGATTTTTTTTTATTTTTTT+TGG + chr5.3:29887920-29887939 MS.gene85871:intron 5.0%
ACAGAATCAACCACAGTCCC+TGG - chr5.3:29890830-29890849 None:intergenic 50.0%
CCGTGTCTATGGCTTAACTG+AGG + chr5.3:29889116-29889135 MS.gene85871:CDS 50.0%
CCTTCTTGTCCACCAGAATC+TGG - chr5.3:29888328-29888347 None:intergenic 50.0%
CGAATTGCCGAGACAAGTGT+CGG + chr5.3:29890197-29890216 MS.gene85871:CDS 50.0%
CTTGAATCGTCGAGAGGATG+AGG - chr5.3:29890416-29890435 None:intergenic 50.0%
GAGTTTCGCTAACACCGTCT+CGG - chr5.3:29890619-29890638 None:intergenic 50.0%
GATCATCCGACAGTTTCCCA+AGG - chr5.3:29889323-29889342 None:intergenic 50.0%
GATTCGGATACAAGCAGCAC+TGG - chr5.3:29890232-29890251 None:intergenic 50.0%
GCAACATCCTTAGAGGGATG+AGG - chr5.3:29888436-29888455 None:intergenic 50.0%
GGGAGAAGCAAAGATATCGG+TGG + chr5.3:29888835-29888854 MS.gene85871:CDS 50.0%
GTTGCAAGGAGACCAGATTC+TGG + chr5.3:29888313-29888332 MS.gene85871:CDS 50.0%
TCAGCTCCCATGAACATCAC+CGG - chr5.3:29890383-29890402 None:intergenic 50.0%
TTGGCAAGGAGAGAGATCAC+AGG - chr5.3:29888352-29888371 None:intergenic 50.0%
! CCAGATTCTGGTGGACAAGA+AGG + chr5.3:29888325-29888344 MS.gene85871:CDS 50.0%
! GATGGACTTGAATCGTCGAG+AGG - chr5.3:29890422-29890441 None:intergenic 50.0%
! GATTTTATCGGGCGCTTTGC+CGG + chr5.3:29888495-29888514 MS.gene85871:CDS 50.0%
!! AAGATGGGCTGTCAAAGACC+GGG + chr5.3:29889360-29889379 MS.gene85871:CDS 50.0%
!! CAAGATGGGCTGTCAAAGAC+CGG + chr5.3:29889359-29889378 MS.gene85871:CDS 50.0%
!! GAAGATTGATCGGTGTCAGC+AGG - chr5.3:29890767-29890786 None:intergenic 50.0%
!! GGACGGTTAAATGGACTACG+TGG + chr5.3:29889630-29889649 MS.gene85871:intron 50.0%
CCAACAACAGCTGCGACAGA+TGG - chr5.3:29890440-29890459 None:intergenic 55.0%
CCATCTGTCGCAGCTGTTGT+TGG + chr5.3:29890437-29890456 MS.gene85871:CDS 55.0%
GAATCAACCACAGTCCCTGG+TGG - chr5.3:29890827-29890846 None:intergenic 55.0%
GAGGAAATGTTGGCCCTACC+AGG + chr5.3:29889415-29889434 MS.gene85871:CDS 55.0%
GAGGAGCCGGTGATGTTCAT+GGG + chr5.3:29890374-29890393 MS.gene85871:CDS 55.0%
GATATCGGTGGAGGAGCTGT+TGG + chr5.3:29888847-29888866 MS.gene85871:CDS 55.0%
GCAAGGAGACCAGATTCTGG+TGG + chr5.3:29888316-29888335 MS.gene85871:CDS 55.0%
GCGCTTTGCCGGCATATGAT+GGG + chr5.3:29888506-29888525 MS.gene85871:CDS 55.0%
GGAGGAGCTGTTGGATTGAG+AGG + chr5.3:29888856-29888875 MS.gene85871:CDS 55.0%
GGGGTGTGAAGGGAACAAGT+AGG + chr5.3:29890894-29890913 MS.gene85871:CDS 55.0%
TGAGGAGCCGGTGATGTTCA+TGG + chr5.3:29890373-29890392 MS.gene85871:CDS 55.0%
! CTTTGCCGGCATATGATGGG+AGG + chr5.3:29888509-29888528 MS.gene85871:CDS 55.0%
! GCAGATCTCGGTGCAATGGT+TGG + chr5.3:29890533-29890552 MS.gene85871:CDS 55.0%
CATTGGCGATCGTGACGCGA+AGG - chr5.3:29889864-29889883 None:intergenic 60.0%
CGAAGGGATTCCACTTCCGC+AGG + chr5.3:29888723-29888742 MS.gene85871:CDS 60.0%
CTTCGCGTCACGATCGCCAA+TGG + chr5.3:29889862-29889881 MS.gene85871:CDS 60.0%
GGCGCTTTGCCGGCATATGA+TGG + chr5.3:29888505-29888524 MS.gene85871:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 29887099 29891207 29887099 ID=MS.gene85871
chr5.3 mRNA 29887099 29891207 29887099 ID=MS.gene85871.t1;Parent=MS.gene85871
chr5.3 exon 29887099 29887456 29887099 ID=MS.gene85871.t1.exon1;Parent=MS.gene85871.t1
chr5.3 CDS 29887099 29887456 29887099 ID=cds.MS.gene85871.t1;Parent=MS.gene85871.t1
chr5.3 exon 29888221 29889436 29888221 ID=MS.gene85871.t1.exon2;Parent=MS.gene85871.t1
chr5.3 CDS 29888221 29889436 29888221 ID=cds.MS.gene85871.t1;Parent=MS.gene85871.t1
chr5.3 exon 29889740 29891207 29889740 ID=MS.gene85871.t1.exon3;Parent=MS.gene85871.t1
chr5.3 CDS 29889740 29891207 29889740 ID=cds.MS.gene85871.t1;Parent=MS.gene85871.t1
Gene Sequence

>MS.gene85871

ATGGAACAACAAGAAAACTCAACCAACAAAAACACTAACCAAAATCTCACAAACAAAAATGGAACAAACTCTCATCAACATCATCATCATTACTATCATCACCCTCAACAGCAACAACAACAGTATCAGTATCAGAATCATCTTCAACAATACCAAACTCAACTTGGTTTCTACAACAACTACCAAAACAAGTTTCAAAGATATTACCCTGCTCTTCTTCCTTTACCTTCACTTCAACAACTACCTTTGATTCCATCTTTCCCTCAAAATCTCAAAACCCATTTGCACAAACTTCCATGCAAACTCAATACTTCTCCTTCATCAGAATACAATCTTTCTCAACTTTCACTTGATCCTGGTAAGTAAGCACCATTCTTCACTGTTCTTTTTTTTTTTTGGTTAAGTCCATTCTTCACTGTTCTTTTCATTTCAAACTTACCCTTTTGCTTGCATTCCTTGTTTTTTTTAATATAGATGCTAAAAATTTGTTCTTTAAGCCTTGTTTGTTTGGTTTTTTAAGAAAAAATTTTAAATTTACTTTGACTTATTGGAGAGTGTGAAAAAAATAAATTTTTTATTGTTTCTTACTCTTTTTATATACTTTTTAAACATTGCATTTGGGGTTGTAATAATTTATTATTTATTTATATATTGTCCATTTTTTTTAACTTGAATGAAGCTAGGTTTGATTCAGATTTTACATTGGTTATGCTGTTTTTAATGTGAAAATGTAAGGTCACTGTTTCACTTGCAGCAATTTATCCTTTATGTTTTTGAAACATGACATTTTTTTGTAACTTCAGTGTTATGTTTTTGCTTAGTGATTTTTTTTTATTTTTTTTGGTTAAAAGTAGATTTAATTTAAAGTGAAATGATCACTTGTTTGGTCTCACATCAAGTCAAAATTGATTTATTTTCAAACTTGCTTTAATAAGAAGCAACAATAATTTACTTTTTGCTTTAAGCACTTTTAGAAATTATCCTAATAACATTCTTCACATAAGATAAATCTAACTGTGACATGAAAAAGATACTTAGTTATTTGAGCTTAACAATTAATTTATGCAACATAGTTTATTGCAAAAAAAATGATGAACCGTCTTATTTATGATAATGATTTAGCACCAAAGGAAATTCAGAAGCAAACTAGACCATCCTTTAAAGAAGTTGATGGGAAGAAGCTTATTTCAACAAGGAAGCCACATGAAGTAATTGTTGCAAGGAGACCAGATTCTGGTGGACAAGAAGGTCCTGTGATCTCTCTCCTTGCCAACCATTTTTTGGTGAAGTTTGATTCATCACAAAAAATATATCATTACAATGTTGAAATTACTCCTCATCCCTCTAAGGATGTTGCTAGAGAAATCAAGCATAAATTGGTAAATAACAATGCTGAGATTTTATCGGGCGCTTTGCCGGCATATGATGGGAGGAAAAATCTTTATAGTCCAATTGAATTCCAAAATGATAAGCTTGAGTTCTACATAAGCCTCCCAATTCCAACTAGCAAATCGACATCACCTTATGAGAAACGTGAACAACATAAACTTTTTCGGATAAATATCAAACTAGTTTCGAAGATTGACGGGAAGGGGTTGACTAAATACTTGAGCAAAGAAGGTGACGAAGGGATTCCACTTCCGCAGGATTATCTACATGCTTTGGATGTAGTCTTGAGGGAAAGTCCGACTGAGAAATGTATACCTGTTGGAAGGTCATTCTATTCGAGTTCAATGGGGAGAAGCAAAGATATCGGTGGAGGAGCTGTTGGATTGAGAGGCTTCTTTCAGAGTCTTAGACCAACACAACAAGGACTTGCTCTCAATGTGGATTTCTCGGTAACTGCTTTCCATGAGAGTATAGGAGTTATTCCGTATTTGCAGAAACGCCTCGAGTTTCTTAGAGACCTTTCTCAAAGGCAGACAACTCAGCTAACTTGTGAAGAAAGGAAGGAAGTAGAGAAAACGTTGAAGAACATCAGAGTCTTTGTTTGCCATAGAGAAACCGTTCAGAGATACCGTGTCTATGGCTTAACTGAGGAGGCAACTGAAAATCTTTGGTTTCCTGATAGAGATGGAAAGAATCTGAGGCTTATGAGTTACTTTAAAGATCACTATAACTATGACATTCAATTCAGGAAGTGGCCATGCTTGCAAATTAGTAGGAGTAAACCTTGTTATCTCCCTATGGAGCTTTGTGTGATCTGTGAAGGACAGAAGTTCCTTGGGAAACTGTCGGATGATCAGACGGCAAAAATACTCAAGATGGGCTGTCAAAGACCGGGAGAACGAAAAGCCATCATCGAAGGTGTCATGAGAGGAAATGTTGGCCCTACCAGGTATAACTGCAATGCAGCACTTTCATATTTCTTGTTTATAGAATGCTGTGTATGATTCTTAGTACTTCTATTTTATGATCATTTATGTATTTGAGTGAAATCTCATTCTGATTCCTCATAAAAACCAGAACACTGGAGTATCTTAACCAACATCCATTCACAAACCGTGAAACCGTTTATTAAGTGATTACGTGGACGGTTAAATGGACTACGTGGCATTAACAGCGTTTAATTGATTATTACATTGTTTTTGAATTAGTTTCTTTCTACTTTCTAATGCTTTAAAAGTAATCCTTTTGACAGTGGTAACCAGGAAAATGAATTCAAACTCCAAGTCTCAAGAGAAATGACAAAGTTGACTGGGAGAATTCTTTACCCTCCAAAACTAAAGCTTGGTGATGGAGGTCATGTGAGAAATCTGACTCCTTCGCGTCACGATCGCCAATGGAACTTTCTAGATGGCCATGTCTTTGAAGGAACTACAATTGAAAGGTGGGCACTAATAAGTTTTGGAGGCACACCTGAGCAAAAGTCTCATATCCCAAGATTCATAAACCAGTTAACTCAAAGATGTGAACAATTAGGCATTTTTCTCAACAAGAACACAATTATTAGTCCGCAGTTTGAATCAATCCAAGTTCTTAACAATGTCACAATTTTGGAATCTAAGCTCAAAAGAATCCAGAGTATTGCCTCAAACAATCTACAGCTTCTTATTTGTATAATGGAGAAAAAACACAAAGGGTATGCAGATTTGAAACGAATTGCCGAGACAAGTGTCGGTGTTGTAAGCCAGTGCTGCTTGTATCCGAATCTCATCAAATTAAGTTCACAGTTTTTGGCTAATTTGGCTCTCAAGATCAATGCCAAAGTTGGTGGATGCACTGTTGCTTTGTACAACTCGCTTCCTTCTCAATTACCGCGTCTTTTCAATATTGAGGAGCCGGTGATGTTCATGGGAGCTGACGTCACGCATCCTCATCCTCTCGACGATTCAAGTCCATCTGTCGCAGCTGTTGTTGGTAGCATGAACTGGCCAACAGCAAACAAATATATTTCAAGAATAAGGTCTCAAACACACAGGCAAGAAATTATTGCAGATCTCGGTGCAATGGTTGGAGAATTGCTTGAAGATTTTTATCAAGAAGTGGAAAAACTCCCAAACCGAATAATTTTCTTCCGAGACGGTGTTAGCGAAACTCAGTTTTACAAAGTTCTGCAAGAGGAACTACAATCAATAAAACAAGCATGTTCATCAAGGTTTCATGGTTATAAACCTTTTATTACTTTTGTAGTTGTGCAAAAAAGGCATCACACAAGGTTGTTTCCTGCTGACACCGATCAATCTTCGACGCACAACCATTTTCACTTTCAATATGAAAATATTCCACCAGGGACTGTGGTTGATTCTGTGATTACTCATCCAAAGGAATTTGATTTCTATCTATGTAGTCATTGGGGTGTGAAGGGAACAAGTAGGCCAACTCATTACCATGTTTTGTGGGATGAAAATAAGTTTACTTCAGATGAACTGCAAAAGCTTGTTTATAATTTGTGTTTTACTTTTGTTAGGTGTACTAAGCCAATTTCATTAGTTCCTCCTGCATATTATGCACATTTAGCTGCATATAGAGGTAGACTCTACCTTGAGAGATCAGAGTCCTTAGGTTTGTTCAGAAGTGCTTCTACATTATCAAGAGCTGCTACGCCGAAGACACCACCTCTACCTAAACTTAGTGAAAACATCAAGAAGCTTATGTTTTACTGCTAG
Protein sequence

>MS.gene85871.t1

MEQQENSTNKNTNQNLTNKNGTNSHQHHHHYYHHPQQQQQQYQYQNHLQQYQTQLGFYNNYQNKFQRYYPALLPLPSLQQLPLIPSFPQNLKTHLHKLPCKLNTSPSSEYNLSQLSLDPAPKEIQKQTRPSFKEVDGKKLISTRKPHEVIVARRPDSGGQEGPVISLLANHFLVKFDSSQKIYHYNVEITPHPSKDVAREIKHKLVNNNAEILSGALPAYDGRKNLYSPIEFQNDKLEFYISLPIPTSKSTSPYEKREQHKLFRINIKLVSKIDGKGLTKYLSKEGDEGIPLPQDYLHALDVVLRESPTEKCIPVGRSFYSSSMGRSKDIGGGAVGLRGFFQSLRPTQQGLALNVDFSVTAFHESIGVIPYLQKRLEFLRDLSQRQTTQLTCEERKEVEKTLKNIRVFVCHRETVQRYRVYGLTEEATENLWFPDRDGKNLRLMSYFKDHYNYDIQFRKWPCLQISRSKPCYLPMELCVICEGQKFLGKLSDDQTAKILKMGCQRPGERKAIIEGVMRGNVGPTSGNQENEFKLQVSREMTKLTGRILYPPKLKLGDGGHVRNLTPSRHDRQWNFLDGHVFEGTTIERWALISFGGTPEQKSHIPRFINQLTQRCEQLGIFLNKNTIISPQFESIQVLNNVTILESKLKRIQSIASNNLQLLICIMEKKHKGYADLKRIAETSVGVVSQCCLYPNLIKLSSQFLANLALKINAKVGGCTVALYNSLPSQLPRLFNIEEPVMFMGADVTHPHPLDDSSPSVAAVVGSMNWPTANKYISRIRSQTHRQEIIADLGAMVGELLEDFYQEVEKLPNRIIFFRDGVSETQFYKVLQEELQSIKQACSSRFHGYKPFITFVVVQKRHHTRLFPADTDQSSTHNHFHFQYENIPPGTVVDSVITHPKEFDFYLCSHWGVKGTSRPTHYHVLWDENKFTSDELQKLVYNLCFTFVRCTKPISLVPPAYYAHLAAYRGRLYLERSESLGLFRSASTLSRAATPKTPPLPKLSENIKKLMFYC