AlfalfaGEDB | MS.gene86550

M. sativa cultivar XinJiangDaYe / MS.gene86550

KEGG: K25507
GO: -
PFAM: PF00505.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86550.t1	XP_003612362.1	99.1	113	1	0	1	113	1	113	2.10E-53	218.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86550.t1	P40619	63.5	96	34	1	11	105	10	105	1.4e-26	120.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86550.t1	G7K181	99.1	113	1	0	1	113	1	113	1.5e-53	218.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene86550.t1	TR	HMG

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04985	MS.gene86550	0.805956	1.04E-49	-1.69E-46
MS.gene049989	MS.gene86550	0.827207	1.78E-54	-1.69E-46
MS.gene051263	MS.gene86550	0.841428	4.78E-58	-1.69E-46
MS.gene051478	MS.gene86550	0.81156	6.59E-51	-1.69E-46
MS.gene053348	MS.gene86550	0.801669	8.08E-49	-1.69E-46
MS.gene055957	MS.gene86550	0.881287	2.72E-70	-1.69E-46
MS.gene056041	MS.gene86550	0.834708	2.56E-56	-1.69E-46
MS.gene056042	MS.gene86550	0.853405	2.44E-61	-1.69E-46
MS.gene056228	MS.gene86550	0.819401	1.18E-52	-1.69E-46
MS.gene056335	MS.gene86550	0.825103	5.63E-54	-1.69E-46
MS.gene057458	MS.gene86550	0.807896	4.04E-50	-1.69E-46
MS.gene058196	MS.gene86550	0.801843	7.44E-49	-1.69E-46
MS.gene058197	MS.gene86550	0.81382	2.11E-51	-1.69E-46
MS.gene058198	MS.gene86550	0.817111	3.91E-52	-1.69E-46
MS.gene059300	MS.gene86550	0.819832	9.44E-53	-1.69E-46
MS.gene060473	MS.gene86550	0.810225	1.28E-50	-1.69E-46
MS.gene060565	MS.gene86550	0.80373	3.03E-49	-1.69E-46
MS.gene06057	MS.gene86550	0.815793	7.71E-52	-1.69E-46
MS.gene061099	MS.gene86550	0.819538	1.10E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86550.t1	MTR_5g024180	99.286	140	1	0	1	140	1	140	1.66e-95	271
MS.gene86550.t1	MTR_8g073345	68.421	133	39	2	1	132	1	131	1.97e-56	172
MS.gene86550.t1	MTR_8g073345	67.969	128	38	2	1	127	1	126	4.25e-53	164
MS.gene86550.t1	MTR_5g024180	94.624	93	2	1	43	132	3	95	1.09e-50	157
MS.gene86550.t1	MTR_3g115580	60.952	105	33	2	6	105	13	114	2.10e-34	117
MS.gene86550.t1	MTR_7g068280	60.000	100	39	1	32	131	47	145	2.76e-30	107
MS.gene86550.t1	MTR_7g082820	53.211	109	45	3	28	131	4	111	6.07e-28	100
MS.gene86550.t1	MTR_1g115220	48.485	99	49	1	32	130	98	194	6.08e-25	95.1
MS.gene86550.t1	MTR_0405s0060	94.118	34	2	0	82	115	74	107	2.46e-14	67.8
MS.gene86550.t1	MTR_4g094622	38.272	81	50	0	25	105	53	133	3.81e-14	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86550.t1	AT1G20693	62.903	124	40	3	18	139	23	142	5.09e-42	136
MS.gene86550.t1	AT1G20693	64.545	110	35	2	18	127	23	128	3.74e-38	126
MS.gene86550.t1	AT1G20693	64.545	110	35	2	18	127	23	128	3.85e-38	126
MS.gene86550.t1	AT2G17560	50.769	130	61	1	6	135	8	134	1.06e-37	125
MS.gene86550.t1	AT1G20696	66.327	98	32	1	30	127	29	125	5.88e-37	123
MS.gene86550.t1	AT2G17560	52.033	123	56	1	6	128	8	127	7.88e-37	123
MS.gene86550.t1	AT2G17560	52.033	123	56	1	6	128	8	127	7.88e-37	123
MS.gene86550.t1	AT3G51880	61.468	109	37	3	24	131	40	144	1.98e-35	120
MS.gene86550.t1	AT3G51880	61.468	109	37	3	24	131	40	144	1.98e-35	120
MS.gene86550.t1	AT3G51880	61.468	109	37	3	24	131	40	144	1.98e-35	120
MS.gene86550.t1	AT3G51880	61.468	109	37	3	24	131	40	144	2.38e-35	120
MS.gene86550.t1	AT1G20696	72.368	76	21	0	30	105	29	104	6.91e-34	116
MS.gene86550.t1	AT1G20696	72.368	76	21	0	30	105	29	104	8.14e-34	115
MS.gene86550.t1	AT3G51880	60.952	105	36	3	24	127	40	140	4.71e-33	114
MS.gene86550.t1	AT4G35570	47.222	108	53	1	7	114	9	112	9.20e-30	104
MS.gene86550.t1	AT4G35570	47.222	108	53	1	7	114	9	112	9.20e-30	104
MS.gene86550.t1	AT1G20696	56.842	95	38	2	44	136	1	94	1.30e-26	95.9
MS.gene86550.t1	AT1G20696	57.143	84	35	1	44	127	1	83	8.54e-24	89.0
MS.gene86550.t1	AT5G23420	46.667	75	37	2	28	101	107	179	7.66e-14	66.2
MS.gene86550.t1	AT4G23800	49.315	73	34	1	32	104	251	320	1.06e-12	64.3
MS.gene86550.t1	AT4G23800	41.892	74	42	1	32	105	369	441	8.83e-12	61.6
MS.gene86550.t1	AT4G23800	49.315	73	34	1	32	104	251	320	1.14e-12	64.3
MS.gene86550.t1	AT4G23800	41.892	74	42	1	32	105	375	447	9.29e-12	61.6
MS.gene86550.t1	AT2G34450	35.714	70	45	0	36	105	63	132	2.79e-12	60.8
MS.gene86550.t1	AT2G34450	35.714	70	45	0	36	105	63	132	2.79e-12	60.8
MS.gene86550.t1	AT2G34450	35.714	70	45	0	36	105	63	132	2.79e-12	60.8
MS.gene86550.t1	AT2G34450	36.620	71	44	1	36	105	63	133	8.07e-12	59.3
MS.gene86550.t1	AT4G11080	42.553	94	45	2	32	125	242	326	8.07e-12	61.6

Find 35 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACAATGCGTGCTTACAATA+TGG	0.281097	5.1:+14839621	MS.gene86550:CDS
GGAGGCCTCTTAGGCTTGTT+AGG	0.297257	5.1:-14839030	None:intergenic
AAAGCACTTGGAGGCCTCTT+AGG	0.299005	5.1:-14839039	None:intergenic
TTGAATTAGGTTGGGAAAGC+TGG	0.350890	5.1:+14839417	MS.gene86550:intron
AAGCGCAAGGGCGCTGGTAC+TGG	0.369054	5.1:+14838979	MS.gene86550:CDS
TTATCAGCCTTAGCATTGAA+TGG	0.428927	5.1:-14839577	None:intergenic
ACAGCAACGGACTTGTTGCT+TGG	0.433440	5.1:-14839307	None:intergenic
TTAGCATCGGAAACAGCTTT+CGG	0.437399	5.1:-14838840	None:intergenic
ATGCTAAACCTGCTGATAAC+CGG	0.456641	5.1:+14838856	MS.gene86550:CDS
ACAATGCGTGCTTACAATAT+GGG	0.459061	5.1:+14839622	MS.gene86550:CDS
ATCATCATTCACTTCAGACT+TGG	0.478350	5.1:-14839791	None:intergenic
TGCTAAGGCTGATAAGTTGA+AGG	0.485540	5.1:+14839585	MS.gene86550:CDS
GAATCTCACCGGTTATCAGC+AGG	0.496399	5.1:-14838864	None:intergenic
GTTCAGAGAGAGGTTTAAGA+AGG	0.508587	5.1:+14839279	MS.gene86550:CDS
GGAAAGCTGGTGGAAAGGAA+TGG	0.519414	5.1:+14839430	MS.gene86550:CDS
AGGTTGAAGCGCAAGGGCGC+TGG	0.524589	5.1:+14838973	MS.gene86550:intron
GTTTGTTAGGTTGAAGCGCA+AGG	0.526395	5.1:+14838966	MS.gene86550:intron
AAGAATGCTTCTGAGGAAGA+AGG	0.530721	5.1:+14839757	MS.gene86550:CDS
AATTAGGTTGGGAAAGCTGG+TGG	0.535794	5.1:+14839420	MS.gene86550:intron
GGTTGGGAAAGCTGGTGGAA+AGG	0.549667	5.1:+14839425	MS.gene86550:intron
AGGATCCTAACAAGCCTAAG+AGG	0.555088	5.1:+14839025	MS.gene86550:CDS
AAAACGAAGAAAGCACTTGG+AGG	0.557236	5.1:-14839048	None:intergenic
TTTGTTAGGTTGAAGCGCAA+GGG	0.561375	5.1:+14838967	MS.gene86550:intron
TAAGGCTCCATTCAATGCTA+AGG	0.574542	5.1:+14839570	MS.gene86550:CDS
GACTGAGAAGAATGCTTCTG+AGG	0.577758	5.1:+14839750	MS.gene86550:intron
ATGAAAACGAAGAAAGCACT+TGG	0.589018	5.1:-14839051	None:intergenic
GTGAATGATGATGATGAGGA+TGG	0.602793	5.1:+14839802	MS.gene86550:CDS
AAGCAACAAGTCCGTTGCTG+TGG	0.608709	5.1:+14839309	MS.gene86550:CDS
GTTATCAGCAGGTTTAGCAT+CGG	0.618036	5.1:-14838853	None:intergenic
TTAGGTCTGAGTTCAGAGAG+AGG	0.622758	5.1:+14839269	MS.gene86550:intron
CAGAAATCTCACCACAGCAA+CGG	0.627421	5.1:-14839320	None:intergenic
ATCGGAAACAGCTTTCGGCA+TGG	0.634546	5.1:-14838835	None:intergenic
TGAAGTGAATGATGATGATG+AGG	0.653732	5.1:+14839798	MS.gene86550:CDS
TAAGCAATCGAAGAAAGCGA+AGG	0.658279	5.1:+14839005	MS.gene86550:CDS
TAAGGCTGATAAGTTGAAGG+AGG	0.688265	5.1:+14839588	MS.gene86550:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTATTATTCTATAATTTTTT+AGG	+	chr5.1:14839853-14839872	MS.gene86550:intron	10.0%
!!	TTCATAAAAATCAAATTCAA+CGG	-	chr5.1:14839363-14839382	None:intergenic	15.0%
!!!	TTTCATTATTTTATGTTTTC+AGG	+	chr5.1:14839897-14839916	MS.gene86550:intron	15.0%
!!!	TTTTTGATTTTTTGTTTGTT+AGG	+	chr5.1:14838953-14838972	MS.gene86550:intron	15.0%
!!!	ATTTTTGTTTTGCGTTTTTT+AGG	+	chr5.1:14839251-14839270	MS.gene86550:intron	20.0%
!!!	TTGTGATTTATGTGTATTTT+AGG	+	chr5.1:14839546-14839565	MS.gene86550:intron	20.0%
!!!	TTTATGTGTATTTTAGGATA+AGG	+	chr5.1:14839552-14839571	MS.gene86550:intron	20.0%
!	AGAATCAAATCAAACTTCAA+CGG	-	chr5.1:14839227-14839246	None:intergenic	25.0%
!!	CTAAATCTTAATTAGCGTTT+TGG	+	chr5.1:14839509-14839528	MS.gene86550:intron	25.0%
!!	TCTAACGTGATTTTTGAATT+AGG	+	chr5.1:14839404-14839423	MS.gene86550:intron	25.0%
!!!	CATTATTTTATGTTTTCAGG+AGG	+	chr5.1:14839900-14839919	MS.gene86550:intron	25.0%
!!!	TTGCTGATTTGAACTAATTT+TGG	+	chr5.1:14839720-14839739	MS.gene86550:intron	25.0%
	CCAAAACTCAATAAACGAAA+AGG	-	chr5.1:14839177-14839196	None:intergenic	30.0%
!	ACGTGATTTTTGAATTAGGT+TGG	+	chr5.1:14839408-14839427	MS.gene86550:intron	30.0%
!	CCTTTTCGTTTATTGAGTTT+TGG	+	chr5.1:14839174-14839193	MS.gene86550:intron	30.0%
!	CGTGATTTTTGAATTAGGTT+GGG	+	chr5.1:14839409-14839428	MS.gene86550:intron	30.0%
!	CTTCGTTTTCATGTAAGATT+CGG	+	chr5.1:14839062-14839081	MS.gene86550:intron	30.0%
!	TTCACTGTTCTGATTTTACT+CGG	+	chr5.1:14839686-14839705	MS.gene86550:intron	30.0%
!!	TATTTTATGTTTTCAGGAGG+AGG	+	chr5.1:14839903-14839922	MS.gene86550:intron	30.0%
	ACAATGCGTGCTTACAATAT+GGG	+	chr5.1:14839622-14839641	MS.gene86550:CDS	35.0%
	ATCATCATTCACTTCAGACT+TGG	-	chr5.1:14839794-14839813	None:intergenic	35.0%
	GTGCTAAAAGTTGTTCGAAT+CGG	+	chr5.1:14839084-14839103	MS.gene86550:intron	35.0%
!	TGAAGTGAATGATGATGATG+AGG	+	chr5.1:14839798-14839817	MS.gene86550:CDS	35.0%
!	TTATCAGCCTTAGCATTGAA+TGG	-	chr5.1:14839580-14839599	None:intergenic	35.0%
!!	ATGAAAACGAAGAAAGCACT+TGG	-	chr5.1:14839054-14839073	None:intergenic	35.0%
	AGAATCACGAATCTCGAAGT+CGG	-	chr5.1:14838895-14838914	None:intergenic	40.0%
	ATGCTAAACCTGCTGATAAC+CGG	+	chr5.1:14838856-14838875	MS.gene86550:CDS	40.0%
	GACAATGCGTGCTTACAATA+TGG	+	chr5.1:14839621-14839640	MS.gene86550:CDS	40.0%
	GTTATCAGCAGGTTTAGCAT+CGG	-	chr5.1:14838856-14838875	None:intergenic	40.0%
	GTTCAGAGAGAGGTTTAAGA+AGG	+	chr5.1:14839279-14839298	MS.gene86550:CDS	40.0%
	TAAGCAATCGAAGAAAGCGA+AGG	+	chr5.1:14839005-14839024	MS.gene86550:CDS	40.0%
	TAAGGCTCCATTCAATGCTA+AGG	+	chr5.1:14839570-14839589	MS.gene86550:CDS	40.0%
	TCGAGATTCGTGATTCTCAT+CGG	+	chr5.1:14838898-14838917	MS.gene86550:intron	40.0%
!	AAGAATGCTTCTGAGGAAGA+AGG	+	chr5.1:14839757-14839776	MS.gene86550:CDS	40.0%
!	GTGAATGATGATGATGAGGA+TGG	+	chr5.1:14839802-14839821	MS.gene86550:CDS	40.0%
!	TAAGGCTGATAAGTTGAAGG+AGG	+	chr5.1:14839588-14839607	MS.gene86550:CDS	40.0%
!	TGCTAAGGCTGATAAGTTGA+AGG	+	chr5.1:14839585-14839604	MS.gene86550:CDS	40.0%
!	TTAGCATCGGAAACAGCTTT+CGG	-	chr5.1:14838843-14838862	None:intergenic	40.0%
!!	AAAACGAAGAAAGCACTTGG+AGG	-	chr5.1:14839051-14839070	None:intergenic	40.0%
!!	TTGAATTAGGTTGGGAAAGC+TGG	+	chr5.1:14839417-14839436	MS.gene86550:intron	40.0%
!!	TTTGTTAGGTTGAAGCGCAA+GGG	+	chr5.1:14838967-14838986	MS.gene86550:intron	40.0%
	AGGATCCTAACAAGCCTAAG+AGG	+	chr5.1:14839025-14839044	MS.gene86550:CDS	45.0%
	AGTCGGAAGAAGAATCTCAC+CGG	-	chr5.1:14838878-14838897	None:intergenic	45.0%
	CAGAAATCTCACCACAGCAA+CGG	-	chr5.1:14839323-14839342	None:intergenic	45.0%
	GACTGAGAAGAATGCTTCTG+AGG	+	chr5.1:14839750-14839769	MS.gene86550:intron	45.0%
	TTAGGTCTGAGTTCAGAGAG+AGG	+	chr5.1:14839269-14839288	MS.gene86550:intron	45.0%
!!	AATTAGGTTGGGAAAGCTGG+TGG	+	chr5.1:14839420-14839439	MS.gene86550:intron	45.0%
!!	ATGTTTTCAGGAGGAGGATG+AGG	+	chr5.1:14839909-14839928	MS.gene86550:intron	45.0%
!!	GTTTGTTAGGTTGAAGCGCA+AGG	+	chr5.1:14838966-14838985	MS.gene86550:intron	45.0%
	AAGCAACAAGTCCGTTGCTG+TGG	+	chr5.1:14839309-14839328	MS.gene86550:CDS	50.0%
	AAGCTCTGTCTGATGCTGTG+AGG	+	chr5.1:14839454-14839473	MS.gene86550:intron	50.0%
	GGAAAGCTGGTGGAAAGGAA+TGG	+	chr5.1:14839430-14839449	MS.gene86550:CDS	50.0%
!	AAAGCACTTGGAGGCCTCTT+AGG	-	chr5.1:14839042-14839061	None:intergenic	50.0%
!	GAATCTCACCGGTTATCAGC+AGG	-	chr5.1:14838867-14838886	None:intergenic	50.0%
!!	ACAGCAACGGACTTGTTGCT+TGG	-	chr5.1:14839310-14839329	None:intergenic	50.0%
	GGTTGGGAAAGCTGGTGGAA+AGG	+	chr5.1:14839425-14839444	MS.gene86550:intron	55.0%
!	GGAGGCCTCTTAGGCTTGTT+AGG	-	chr5.1:14839033-14839052	None:intergenic	55.0%
!	AGGTTGAAGCGCAAGGGCGC+TGG	+	chr5.1:14838973-14838992	MS.gene86550:intron	65.0%
!!	AAGCGCAAGGGCGCTGGTAC+TGG	+	chr5.1:14838979-14838998	MS.gene86550:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	14838837	14839936	14838837	ID=MS.gene86550
chr5.1	mRNA	14838837	14839936	14838837	ID=MS.gene86550.t1;Parent=MS.gene86550
chr5.1	exon	14838837	14838877	14838837	ID=MS.gene86550.t1.exon1;Parent=MS.gene86550.t1
chr5.1	CDS	14838837	14838877	14838837	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14838975	14839073	14838975	ID=MS.gene86550.t1.exon2;Parent=MS.gene86550.t1
chr5.1	CDS	14838975	14839073	14838975	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14839273	14839330	14839273	ID=MS.gene86550.t1.exon3;Parent=MS.gene86550.t1
chr5.1	CDS	14839273	14839330	14839273	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14839426	14839470	14839426	ID=MS.gene86550.t1.exon4;Parent=MS.gene86550.t1
chr5.1	CDS	14839426	14839470	14839426	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14839568	14839648	14839568	ID=MS.gene86550.t1.exon5;Parent=MS.gene86550.t1
chr5.1	CDS	14839568	14839648	14839568	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14839751	14839831	14839751	ID=MS.gene86550.t1.exon6;Parent=MS.gene86550.t1
chr5.1	CDS	14839751	14839831	14839751	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1
chr5.1	exon	14839919	14839936	14839919	ID=MS.gene86550.t1.exon7;Parent=MS.gene86550.t1
chr5.1	CDS	14839919	14839936	14839919	ID=cds.MS.gene86550.t1;Parent=MS.gene86550.t1

Gene Sequence

>MS.gene86550

ATGCCGAAAGCTGTTTCCGATGCTAAACCTGCTGATAACCGGTGAGATTCTTCTTCCGACTTCGAGATTCGTGATTCTCATCGGTTAATTTGTTAGATCTGAATTTTTTGTTAACGTTTTTGATTTTTTGTTTGTTAGGTTGAAGCGCAAGGGCGCTGGTACTGGAACTAAGCAATCGAAGAAAGCGAAGGATCCTAACAAGCCTAAGAGGCCTCCAAGTGCTTTCTTCGTTTTCATGTAAGATTCGGTGCTAAAAGTTGTTCGAATCGGTGCTAATTGTTCACGAATTTGTTGCAGTGAAGTTTCATTTAATTCTGACGAGTTTCGTTTTTTTTTTCCTTTTCGTTTATTGAGTTTTGGTGAAGTTCGAAACGTTACGAATGTGTTCCGTTGAAGTTTGATTTGATTCTAATGATTTTTGTTTTGCGTTTTTTAGGTCTGAGTTCAGAGAGAGGTTTAAGAAGGAGAATCCAAGCAACAAGTCCGTTGCTGTGGTGAGATTTCTGACACGATCTAGTTTTTACCGTTGAATTTGATTTTTATGAAATTTTGAATGTTATTGAGTTTTCTAACGTGATTTTTGAATTAGGTTGGGAAAGCTGGTGGAAAGGAATGGAAAGCTCTGTCTGATGCTGTGAGGATTTCGCTTCTGTTTAACTGAAGAAAAATAATCTAAATCTTAATTAGCGTTTTGGTTTTAAAATCGTTTTTGTGATTTATGTGTATTTTAGGATAAGGCTCCATTCAATGCTAAGGCTGATAAGTTGAAGGAGGAGTATGAGAAGACAATGCGTGCTTACAATATGGGAATTGTAATTACTTTTATAACTTTTCTGTTACTTTGATTTTTTCACTGTTCTGATTTTACTCGGATTTTTTTTTGTTGCTGATTTGAACTAATTTTGGTGTATTAGACTGAGAAGAATGCTTCTGAGGAAGAAGGATCTGAGAAGTCCAAGTCTGAAGTGAATGATGATGATGAGGATGGTGATGATGTAAGATTTTTCTCATCGTTTGTATTATTCTATAATTTTTTAGGTTTTTATATATTGAAATTTGATTTCATTATTTTATGTTTTCAGGAGGAGGATGAGGAGTAA

Protein sequence

>MS.gene86550.t1

MPKAVSDAKPADNRLKRKGAGTGTKQSKKAKDPNKPKRPPSAFFVFMSEFRERFKKENPSNKSVAVVGKAGGKEWKALSDADKAPFNAKADKLKEEYEKTMRAYNMGITEKNASEEEGSEKSKSEVNDDDEDGDDEEDEE