AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86827

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86827.t1 MTR_1g077980 96.746 922 29 1 1 921 1 922 0.0 1847
MS.gene86827.t1 MTR_7g088760 48.955 909 432 14 19 913 14 904 0.0 866
MS.gene86827.t1 MTR_7g088760 48.056 926 432 15 19 913 14 921 0.0 856
MS.gene86827.t1 MTR_2g087710 45.890 815 376 12 120 915 99 867 0.0 660
MS.gene86827.t1 MTR_7g106570 44.903 824 401 15 120 920 101 894 0.0 625
MS.gene86827.t1 MTR_1g014260 33.143 175 91 6 756 907 351 522 6.09e-22 102
MS.gene86827.t1 MTR_1g014260 28.723 188 114 3 317 504 179 346 7.20e-15 79.3
MS.gene86827.t1 MTR_4g115880 31.737 167 88 5 764 907 415 578 1.36e-20 97.4
MS.gene86827.t1 MTR_4g115880 30.144 209 97 5 317 497 195 382 2.14e-16 84.0
MS.gene86827.t1 MTR_6g022310 38.312 154 89 5 760 912 280 428 1.31e-19 94.7
MS.gene86827.t1 MTR_6g022310 29.897 194 106 7 319 510 127 292 2.10e-11 68.2
MS.gene86827.t1 MTR_1g014680 38.158 152 87 4 761 909 339 486 2.01e-19 94.4
MS.gene86827.t1 MTR_5g430360 37.908 153 87 6 764 912 136 284 4.40e-18 89.4
MS.gene86827.t1 MTR_5g030360 37.908 153 87 6 764 912 304 452 6.39e-18 89.4
MS.gene86827.t1 MTR_3g100480 34.868 152 92 4 761 909 741 888 1.04e-17 89.0
MS.gene86827.t1 MTR_3g083600 31.837 245 130 6 264 499 170 386 1.82e-17 88.2
MS.gene86827.t1 MTR_3g083600 35.338 133 70 4 792 909 938 1069 1.52e-15 82.0
MS.gene86827.t1 MTR_5g032380 36.242 149 88 4 764 909 582 726 3.37e-17 87.4
MS.gene86827.t1 MTR_6g022300 37.333 150 88 5 764 912 224 368 4.01e-17 86.7
MS.gene86827.t1 MTR_8g090190 32.203 177 99 6 750 911 194 364 7.31e-17 84.0
MS.gene86827.t1 MTR_6g022290 36.667 150 89 5 764 912 186 330 8.36e-17 85.5
MS.gene86827.t1 MTR_6g022290 27.551 196 108 6 319 510 29 194 2.00e-11 68.2
MS.gene86827.t1 MTR_2g071620 34.228 149 91 4 764 909 625 769 8.67e-17 85.9
MS.gene86827.t1 MTR_4g130940 36.424 151 91 5 761 909 248 395 2.33e-16 84.3
MS.gene86827.t1 MTR_4g130940 36.424 151 91 5 761 909 248 395 3.10e-16 84.0
MS.gene86827.t1 MTR_8g067060 37.162 148 85 5 769 912 357 500 1.27e-15 81.6
MS.gene86827.t1 MTR_8g471100 37.162 148 85 5 769 912 366 509 1.46e-15 81.3
MS.gene86827.t1 MTR_5g066000 31.579 152 96 5 761 909 699 845 6.36e-15 80.1
MS.gene86827.t1 MTR_3g087140 29.050 179 106 5 750 913 194 366 7.03e-15 77.8
MS.gene86827.t1 MTR_3g100480 30.556 180 92 5 761 909 741 918 2.63e-14 78.2
MS.gene86827.t1 MTR_0279s0020 37.209 129 75 4 785 912 163 286 2.71e-14 75.1
MS.gene86827.t1 MTR_0279s0020 37.302 126 73 4 785 909 172 292 1.39e-13 73.6
MS.gene86827.t1 MTR_2g070390 32.886 149 94 5 764 910 185 329 2.00e-11 68.2
MS.gene86827.t1 MTR_3g108660 33.846 130 82 3 764 890 255 383 3.78e-11 67.8
MS.gene86827.t1 MTR_3g108660 33.846 130 82 3 764 890 255 383 5.26e-11 67.4
MS.gene86827.t1 MTR_1g010040 31.538 130 85 3 764 890 255 383 9.08e-11 66.6
MS.gene86827.t1 MTR_1g010040 31.538 130 85 3 764 890 255 383 9.82e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86827.t1 AT4G10570 62.353 935 305 11 1 919 1 904 0.0 1140
MS.gene86827.t1 AT4G10590 62.339 932 305 12 1 917 1 901 0.0 1133
MS.gene86827.t1 AT4G10590 62.339 932 305 12 1 917 1 901 0.0 1133
MS.gene86827.t1 AT4G10590 62.339 932 305 12 1 917 1 901 0.0 1133
MS.gene86827.t1 AT4G10590 62.339 932 305 12 1 917 1 901 0.0 1133
MS.gene86827.t1 AT1G32850 60.266 901 316 12 20 913 18 883 0.0 1058
MS.gene86827.t1 AT2G40930 49.727 917 419 16 19 909 15 915 0.0 853
MS.gene86827.t1 AT2G40930 49.727 917 419 16 19 909 15 915 0.0 853
MS.gene86827.t1 AT5G22030 47.767 806 370 17 120 910 100 869 0.0 701
MS.gene86827.t1 AT5G22030 50.000 738 318 17 188 910 210 911 0.0 681
MS.gene86827.t1 AT4G31670 37.908 153 88 4 761 910 326 474 4.18e-20 96.3
MS.gene86827.t1 AT2G24640 37.255 153 89 4 761 910 162 310 5.38e-20 95.1
MS.gene86827.t1 AT5G46740 37.500 152 88 5 764 912 115 262 7.89e-20 94.7
MS.gene86827.t1 AT2G32780 35.533 197 97 6 310 499 189 362 8.99e-20 95.9
MS.gene86827.t1 AT2G32780 37.037 135 65 5 793 910 952 1083 2.55e-14 78.2
MS.gene86827.t1 AT2G24640 36.364 165 95 5 761 919 332 492 1.32e-19 94.7
MS.gene86827.t1 AT5G46740 36.538 156 92 5 760 912 320 471 1.35e-19 94.7
MS.gene86827.t1 AT1G04860 34.783 184 84 4 318 495 230 383 6.60e-19 92.8
MS.gene86827.t1 AT1G04860 38.931 131 64 6 793 910 830 957 8.76e-14 76.3
MS.gene86827.t1 AT4G17895 36.184 152 90 5 764 912 331 478 2.88e-17 87.4
MS.gene86827.t1 AT5G10790 29.412 170 91 5 764 907 233 399 3.69e-17 85.5
MS.gene86827.t1 AT1G17110 34.899 149 90 4 764 909 603 747 3.71e-17 87.4
MS.gene86827.t1 AT1G17110 34.899 149 90 4 764 909 599 743 3.80e-17 87.0
MS.gene86827.t1 AT1G17110 34.899 149 90 4 764 909 599 743 3.80e-17 87.0
MS.gene86827.t1 AT5G10790 29.412 170 91 5 764 907 362 528 6.87e-17 85.5
MS.gene86827.t1 AT4G24560 34.228 149 90 4 764 909 703 846 2.23e-16 84.7
MS.gene86827.t1 AT5G65450 34.667 150 90 5 764 909 506 651 2.28e-15 81.3
MS.gene86827.t1 AT5G65450 34.667 150 90 5 764 909 487 632 2.68e-15 80.9
MS.gene86827.t1 AT4G39910 33.129 163 89 6 764 911 211 368 2.92e-15 79.0
MS.gene86827.t1 AT2G22310 32.934 167 86 7 764 912 174 332 7.10e-15 77.4
MS.gene86827.t1 AT2G22310 32.934 167 86 7 764 912 169 327 8.29e-15 77.0
MS.gene86827.t1 AT2G22310 32.934 167 86 7 764 912 206 364 1.36e-14 77.0
MS.gene86827.t1 AT5G57990 32.450 151 92 5 760 909 268 409 1.60e-14 78.6
MS.gene86827.t1 AT4G30890 37.288 118 70 4 795 910 436 551 1.59e-12 71.6
MS.gene86827.t1 AT4G30890 37.288 118 70 4 795 910 436 551 1.59e-12 71.6
MS.gene86827.t1 AT4G30890 37.288 118 70 4 795 910 436 551 1.59e-12 71.6
MS.gene86827.t1 AT3G49600 32.143 140 86 4 764 895 253 391 2.76e-11 68.2
MS.gene86827.t1 AT3G49600 32.143 140 86 4 764 895 253 391 2.85e-11 68.2
MS.gene86827.t1 AT3G49600 32.143 140 86 4 764 895 253 391 2.85e-11 68.2
MS.gene86827.t1 AT3G14400 31.788 151 97 5 761 909 188 334 3.38e-11 67.8
MS.gene86827.t1 AT3G14400 28.358 201 110 5 320 511 25 200 5.37e-11 67.0

Find 222 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCCTTGGTTACTTATT+TGG 0.167537 1.1:+58397126 MS.gene86827:CDS
AATTATATTAGGCTTCTATT+TGG 0.193463 1.1:+58393010 MS.gene86827:intron
GTGGAAATTGCCAGAGATTT+TGG 0.202696 1.1:+58397790 MS.gene86827:CDS
GAGCTAGTGTGCAAAGTTAA+AGG 0.260338 1.1:+58392882 MS.gene86827:CDS
GTGAATTAGCACTTGCATTT+GGG 0.290290 1.1:+58393947 MS.gene86827:CDS
TTGAGGAAACTTTGGTCCTC+TGG 0.299288 1.1:+58393976 MS.gene86827:CDS
AATTGTAAAATTAAGCAAAA+AGG 0.300141 1.1:+58392106 MS.gene86827:CDS
CTTCCTGAAGTTCACAAAAC+TGG 0.302951 1.1:+58397520 MS.gene86827:CDS
GCTGATCGTTCCTCTCATTC+TGG 0.315839 1.1:+58393456 MS.gene86827:CDS
GGAATCATTGACTTCAATAA+AGG 0.321302 1.1:+58396221 MS.gene86827:CDS
TTTCCACAAGTCCAGTTTCT+TGG 0.325981 1.1:-58397774 None:intergenic
ATGCAGTCCATCCAATAAAA+AGG 0.328639 1.1:-58395406 None:intergenic
GGTGAATTAGCACTTGCATT+TGG 0.331811 1.1:+58393946 MS.gene86827:CDS
TTAATTTCAGCTTCAGAAAC+AGG 0.340496 1.1:-58398206 None:intergenic
CTCTGCAGCAACCATGCTTC+AGG 0.341873 1.1:-58395996 None:intergenic
ATATAAAGGTCTTTGTTGAT+TGG 0.342748 1.1:+58397452 MS.gene86827:CDS
TTGGAAGAAGACCCTCAATA+TGG 0.349829 1.1:+58397145 MS.gene86827:CDS
AGCAACCATTACGGTGGTTT+AGG 0.350370 1.1:+58397959 MS.gene86827:CDS
GAATAGCGCTCTTCAATGTT+TGG 0.351618 1.1:+58393716 MS.gene86827:CDS
GAACTTCCCCATTCACAATC+TGG 0.353781 1.1:+58397874 MS.gene86827:CDS
AAAACCCAGTGCACATATAA+AGG 0.354307 1.1:+58397438 MS.gene86827:CDS
TGTTCTCTGGTGCAAAGTGC+CGG 0.355262 1.1:-58397322 None:intergenic
ATTATATTAGGCTTCTATTT+GGG 0.367380 1.1:+58393011 MS.gene86827:intron
ATCAACTTCCTTGGCAATGC+TGG 0.378205 1.1:-58391999 None:intergenic
GTCTGTGTTCCTTGCATTGA+GGG 0.381184 1.1:-58397745 None:intergenic
GCCAAGGAAGTTGATTTCTC+AGG 0.383623 1.1:+58392007 MS.gene86827:CDS
GATGGAAGAAGCGACGTCTT+AGG 0.385620 1.1:+58398280 MS.gene86827:CDS
AAGTTCATCACTGACTACTA+TGG 0.386122 1.1:+58393617 MS.gene86827:CDS
ACAAAGAAATAGCTTCTTGC+CGG 0.386336 1.1:-58397561 None:intergenic
CAGACAAGCTACCAAGAAAC+TGG 0.387149 1.1:+58397763 MS.gene86827:CDS
TGGTAATGATAAGTACATAA+AGG 0.388653 1.1:-58395887 None:intergenic
GGTGGAGAGAGGAAGATCTT+TGG 0.388726 1.1:+58397103 MS.gene86827:CDS
AGATCATCTGTATCAATTGC+AGG 0.391184 1.1:+58393528 MS.gene86827:CDS
TCATTCTGGTGTGCATTCAA+TGG 0.399599 1.1:+58393470 MS.gene86827:CDS
AAAGATGACGATTGCTGAAT+CGG 0.402583 1.1:+58388507 None:intergenic
TATCACAGATGCCTTGTCAA+TGG 0.404625 1.1:-58397416 None:intergenic
TGTTGCACCACGTGCGTTCA+AGG 0.406091 1.1:+58394008 MS.gene86827:CDS
AGACAGTAATTGTCATTGTC+CGG 0.406135 1.1:-58395927 None:intergenic
TCAATCACCTTTAGATTAAG+TGG 0.406422 1.1:-58392062 None:intergenic
CAGAGATCTTTGCCAAGCAT+TGG 0.409945 1.1:+58396014 MS.gene86827:CDS
GTGCAAAGTGCCGGGTTCCT+CGG 0.410838 1.1:-58397313 None:intergenic
TTCTCTTCCTCCGGTGAAAT+CGG 0.411393 1.1:-58388562 None:intergenic
AGAAATATTGCATCGACATC+TGG 0.412333 1.1:+58396299 MS.gene86827:CDS
TCTCATGAAGGGAAGGAAAC+TGG 0.414310 1.1:+58397235 MS.gene86827:CDS
ATTATATCAGAATTATCAAT+TGG 0.417193 1.1:-58391533 None:intergenic
CACTGAATTGTGGAGCAAAT+CGG 0.419702 1.1:-58394041 None:intergenic
AGTTTGCATTCTCCAATGTC+TGG 0.419774 1.1:-58393077 None:intergenic
AATGGTTGCTGATTGCATAA+AGG 0.420321 1.1:-58397946 None:intergenic
CAAGTTGGCAGAGGTATGTT+GGG 0.420787 1.1:+58391420 MS.gene86827:CDS
ATCACAGATGCCTTGTCAAT+GGG 0.425512 1.1:-58397415 None:intergenic
GAGTCCTTCATTTCAATGTA+AGG 0.429050 1.1:-58395456 None:intergenic
AATTCTGATATAATTTCAAA+TGG 0.429572 1.1:+58391542 MS.gene86827:CDS
TTTAGCAGGACTGCAGAACT+TGG 0.431003 1.1:+58393677 MS.gene86827:CDS
GATGATCTTGGAGGTTCTAA+TGG 0.431147 1.1:-58393513 None:intergenic
GGATGTCCCCTCTTGTTCTC+TGG 0.432883 1.1:-58397335 None:intergenic
TCGGAGCTTAATTTGAAAGA+TGG 0.434560 1.1:+58388613 MS.gene86827:CDS
GTTCTCTGGTGCAAAGTGCC+GGG 0.438834 1.1:-58397321 None:intergenic
TATACTTTATTATTTCCAAT+AGG 0.439148 1.1:+58388641 MS.gene86827:CDS
AATGGTCACTCAAGTGGGTC+TGG 0.439704 1.1:+58393567 MS.gene86827:CDS
AAGACGTCGCTTCTTCCATC+TGG 0.440402 1.1:-58398277 None:intergenic
GGGACTTGTTGAGGAAACTT+TGG 0.440723 1.1:+58393968 MS.gene86827:CDS
GGTAATGATAAGTACATAAA+GGG 0.444185 1.1:-58395886 None:intergenic
ATCAACATGGAGAATCCTTT+AGG 0.444926 1.1:+58393789 MS.gene86827:CDS
GCAGGCCATCAGAAGATTGC+TGG 0.446316 1.1:-58391469 None:intergenic
CAATCTTACCACATGTCATC+TGG 0.451523 1.1:-58397625 None:intergenic
ACGATCCGTTTCTCTTCCTC+CGG 0.451906 1.1:-58388571 None:intergenic
GACATTGGAGAATGCAAACT+TGG 0.453854 1.1:+58393080 MS.gene86827:CDS
ACTGAATTGTGGAGCAAATC+GGG 0.454165 1.1:-58394040 None:intergenic
AAGGAAACTGGTTTCATCTC+AGG 0.457552 1.1:+58397247 MS.gene86827:CDS
ATCAGAATTATCAATTGGAC+CGG 0.457774 1.1:-58391528 None:intergenic
GCAATTGATACAGATGATCT+TGG 0.458422 1.1:-58393525 None:intergenic
AGAGATCTTTGCCAAGCATT+GGG 0.458789 1.1:+58396015 MS.gene86827:CDS
ACCTCAATGCTGTACTGCTT+CGG 0.459105 1.1:-58392035 None:intergenic
ATCTGGTCCTAGAGGTTCTT+CGG 0.459925 1.1:-58397608 None:intergenic
GGTCAAATCCAGATTGTGAA+TGG 0.461365 1.1:-58397882 None:intergenic
CATTCTGGTGTGCATTCAAT+GGG 0.461609 1.1:+58393471 MS.gene86827:CDS
GGTGAAAGAGGTGGTTTAGC+AGG 0.462978 1.1:+58393663 MS.gene86827:CDS
TCAATGTTGGCTCCATATTG+AGG 0.465522 1.1:-58397157 None:intergenic
CAAGTATGGGAAAGGCTGTT+GGG 0.466815 1.1:+58391641 MS.gene86827:CDS
GTCAAATCCAGATTGTGAAT+GGG 0.468661 1.1:-58397881 None:intergenic
CTTCGTTAGTTAAAACAAGT+TGG 0.468962 1.1:-58397375 None:intergenic
TATATGATGCTAGCTATTTG+AGG 0.469608 1.1:+58397494 MS.gene86827:CDS
GATAAATATGACGTCTATAG+TGG 0.470173 1.1:+58393642 MS.gene86827:CDS
TCAAGTTGGCAGAGGTATGT+TGG 0.470261 1.1:+58391419 MS.gene86827:CDS
TATGCAATCAGCAACCATTA+CGG 0.474161 1.1:+58397950 MS.gene86827:CDS
TAAAGGTTATACACATAGGA+TGG 0.475468 1.1:-58397929 None:intergenic
AAAGACCTTTATATGTGCAC+TGG 0.477243 1.1:-58397443 None:intergenic
CAATGTTGGCTCCATATTGA+GGG 0.480052 1.1:-58397156 None:intergenic
CGGAGCTTAATTTGAAAGAT+GGG 0.480500 1.1:+58388614 MS.gene86827:CDS
GAAGATCTTTGGAACTCCCT+TGG 0.482950 1.1:+58397114 MS.gene86827:CDS
ATATGATGCTAGCTATTTGA+GGG 0.484776 1.1:+58397495 MS.gene86827:CDS
CCCTGAGAAATCAACTTCCT+TGG 0.487604 1.1:-58392008 None:intergenic
TGAGGAAACTTTGGTCCTCT+GGG 0.491053 1.1:+58393977 MS.gene86827:CDS
TGTCTGTGTTCCTTGCATTG+AGG 0.491807 1.1:-58397746 None:intergenic
AGCATGCAGTGCCCAATGCT+TGG 0.492001 1.1:-58396026 None:intergenic
TGACGTGACTGTCATCGAAA+TGG 0.493653 1.1:-58398181 None:intergenic
TTGCATTTGGGGACTTGTTG+AGG 0.494177 1.1:+58393959 MS.gene86827:CDS
CATTACGGTGGTTTAGGTGG+TGG 0.494345 1.1:+58397965 MS.gene86827:CDS
CCAAGGAAGTTGATTTCTCA+GGG 0.495656 1.1:+58392008 MS.gene86827:CDS
ACAAGTATGGGAAAGGCTGT+TGG 0.500405 1.1:+58391640 MS.gene86827:CDS
TTAGCAGGACTGCAGAACTT+GGG 0.500451 1.1:+58393678 MS.gene86827:CDS
CAACTCATCCCATTATCAAT+CGG 0.501111 1.1:-58388535 None:intergenic
AAAGCCTTACATTGAAATGA+AGG 0.501423 1.1:+58395452 MS.gene86827:CDS
CACTTCCTTCCACTGTCACC+CGG 0.503998 1.1:+58395908 MS.gene86827:CDS
CTCGGATGGTAGGCCGGATG+AGG 0.505210 1.1:+58395476 MS.gene86827:CDS
TTTAGTTCCTCAACAAGTAT+GGG 0.508258 1.1:+58391628 MS.gene86827:CDS
ATTGAAGTCAATGATTCCAA+TGG 0.513982 1.1:-58396216 None:intergenic
ACGTGCGTTCAAGGGAAAGC+TGG 0.515230 1.1:+58394017 MS.gene86827:CDS
CAAAGAAATAGCTTCTTGCC+GGG 0.515685 1.1:-58397560 None:intergenic
GTCCATACACGCATTCCTAA+AGG 0.518820 1.1:-58393804 None:intergenic
CAAATGGAAGTAATTGTGAC+GGG 0.525856 1.1:+58391558 MS.gene86827:CDS
AATGGGAAATGAATTAGCAT+TGG 0.527118 1.1:+58393488 MS.gene86827:CDS
AGTTGGAAGCGACGTCCAGA+TGG 0.527287 1.1:+58398262 MS.gene86827:CDS
GAAGGAGTTGATAAAGCAAT+CGG 0.529422 1.1:+58388594 MS.gene86827:CDS
AACTAAGTCTCATGAAGGGA+AGG 0.531234 1.1:+58397228 MS.gene86827:CDS
TTTAGATATTCATCGACCAT+TGG 0.531240 1.1:+58391586 MS.gene86827:CDS
AGCCTTTCCCATACTTGTTG+AGG 0.532585 1.1:-58391635 None:intergenic
ATTGATTGTTGATGAATGCC+AGG 0.532873 1.1:+58395545 MS.gene86827:CDS
GAAGGACTCGGATGGTAGGC+CGG 0.535738 1.1:+58395470 MS.gene86827:CDS
AGTGAACAGTATCGGCATGC+AGG 0.538726 1.1:-58391487 None:intergenic
GACATATGTACATAGAATGT+TGG 0.539698 1.1:+58397180 MS.gene86827:CDS
ATTGTCCGGGTGACAGTGGA+AGG 0.540739 1.1:-58395913 None:intergenic
GGTGTACCCACTTAATCTAA+AGG 0.542422 1.1:+58392055 MS.gene86827:CDS
AACTAACTTGAGAATCATGC+TGG 0.546603 1.1:-58394071 None:intergenic
AGCTTTCCCTTGAACGCACG+TGG 0.549334 1.1:-58394015 None:intergenic
AAGTATGTGAAAAGTAAAGA+TGG 0.549335 1.1:+58397905 MS.gene86827:CDS
GACGTCTATAGTGGTGAAAG+AGG 0.550637 1.1:+58393651 MS.gene86827:CDS
GACAGTAATTGTCATTGTCC+GGG 0.551707 1.1:-58395926 None:intergenic
AAGAGCGCTATTCATAAAGC+AGG 0.553621 1.1:-58393706 None:intergenic
TCTTCAACTAAGTCTCATGA+AGG 0.553689 1.1:+58397223 MS.gene86827:CDS
TTGTCTAATGGTCACTCAAG+TGG 0.555942 1.1:+58393561 MS.gene86827:CDS
TCATTTGCGACTTCCTCATC+CGG 0.556059 1.1:-58395489 None:intergenic
TGTTCACTGAACAGTGGGAA+GGG 0.557069 1.1:+58397082 MS.gene86827:intron
TGAATTAGCACTTGCATTTG+GGG 0.557302 1.1:+58393948 MS.gene86827:CDS
TTCAGGACAGTCACAGTGTA+CGG 0.560190 1.1:-58395979 None:intergenic
CACCACCTAAACCACCGTAA+TGG 0.561137 1.1:-58397964 None:intergenic
TCGTTATCTAGATAATCCAT+TGG 0.561503 1.1:+58396200 MS.gene86827:CDS
ATTACGGTGGTTTAGGTGGT+GGG 0.561699 1.1:+58397966 MS.gene86827:CDS
ATCGACTCAAGAGTGGAGCC+AGG 0.567147 1.1:+58396265 MS.gene86827:CDS
GATCATCTGTATCAATTGCA+GGG 0.567157 1.1:+58393529 MS.gene86827:CDS
GAACAAAGAGTCATTGCTGA+CGG 0.567578 1.1:+58393047 MS.gene86827:CDS
TGTCTAATGGTCACTCAAGT+GGG 0.570423 1.1:+58393562 MS.gene86827:CDS
AAAGGATGAAGAACATATTG+TGG 0.573464 1.1:+58396239 MS.gene86827:CDS
GAGTTGTGTGCCGATTTCAC+CGG 0.573770 1.1:+58388552 MS.gene86827:CDS
AAGACCTTTATATGTGCACT+GGG 0.574315 1.1:-58397442 None:intergenic
GAACAAGAGGGGACATCCTG+TGG 0.574752 1.1:+58397340 MS.gene86827:CDS
TCAAATGGAAGTAATTGTGA+CGG 0.574962 1.1:+58391557 MS.gene86827:CDS
TAGCAGGACTGCAGAACTTG+GGG 0.576248 1.1:+58393679 MS.gene86827:CDS
TATGGGAAAGGCTGTTGGGA+TGG 0.576501 1.1:+58391645 MS.gene86827:CDS
AGGTATGTTGGGCATTGTGA+TGG 0.577486 1.1:+58391431 MS.gene86827:CDS
AGGAAGTCGCAAATGAGTGT+TGG 0.577729 1.1:+58395496 MS.gene86827:CDS
GTTGCACCACGTGCGTTCAA+GGG 0.578428 1.1:+58394009 MS.gene86827:CDS
TATCTTCAAGTGAACAGTAT+CGG 0.578627 1.1:-58391495 None:intergenic
ATTGATACAGATGATCTTGG+AGG 0.579531 1.1:-58393522 None:intergenic
GGAGAATGCAAACTTGGCAA+TGG 0.580595 1.1:+58393086 MS.gene86827:CDS
TAGTCTTTATCCTCTTCCAA+TGG 0.580945 1.1:-58391602 None:intergenic
TATTCATCGACCATTGGAAG+AGG 0.581116 1.1:+58391592 MS.gene86827:CDS
CGATTTGCTCCACAATTCAG+TGG 0.583237 1.1:+58394042 MS.gene86827:CDS
ATGTACATATGTCTCAATGT+TGG 0.584427 1.1:-58397170 None:intergenic
AACCATTACGGTGGTTTAGG+TGG 0.584463 1.1:+58397962 MS.gene86827:CDS
TTTCACCGGAGGAAGAGAAA+CGG 0.585574 1.1:+58388566 MS.gene86827:CDS
TTCCTCAACAAGTATGGGAA+AGG 0.585591 1.1:+58391633 MS.gene86827:CDS
TTACATTGAAATGAAGGACT+CGG 0.585855 1.1:+58395458 MS.gene86827:CDS
TAGCTGTGAGCCCATTGACA+AGG 0.586420 1.1:+58397405 MS.gene86827:CDS
TGTCATTGTCCGGGTGACAG+TGG 0.589052 1.1:-58395917 None:intergenic
ACTGTGACTGTCCTGAAGCA+TGG 0.592033 1.1:+58395985 MS.gene86827:CDS
GACGGCTTCAGACCAGACAT+TGG 0.593889 1.1:+58393065 MS.gene86827:CDS
ATTCAGCAAGTGGTGGAGTG+TGG 0.594255 1.1:-58393740 None:intergenic
TTGGAAAGATGACTCTCCAC+AGG 0.597434 1.1:-58397356 None:intergenic
TAGGTACTGCCCTCAATGCA+AGG 0.598022 1.1:+58397736 MS.gene86827:intron
GGCTCCACTCTTGAGTCGAT+AGG 0.599098 1.1:-58396262 None:intergenic
TTGTGTGCCGATTTCACCGG+AGG 0.599322 1.1:+58388555 MS.gene86827:CDS
ACAGTCACAGTGTACGGCAT+GGG 0.599364 1.1:-58395973 None:intergenic
AAATGAAGGACTCGGATGGT+AGG 0.599838 1.1:+58395466 MS.gene86827:CDS
TTCTTCCAAATAAGTAACCA+AGG 0.600475 1.1:-58397131 None:intergenic
ACTGACCAGCAATCTTCTGA+TGG 0.604191 1.1:+58391464 MS.gene86827:CDS
CTACCAAGAAACTGGACTTG+TGG 0.605316 1.1:+58397771 MS.gene86827:CDS
ACTTTGCACCAGAGAACAAG+AGG 0.606165 1.1:+58397327 MS.gene86827:CDS
GCATTACACTGCATATGCTA+AGG 0.606988 1.1:+58397988 MS.gene86827:CDS
AGTTGATTGATGATAATAAG+TGG 0.607365 1.1:+58398156 MS.gene86827:intron
TCACAGTGAACCGAGGAACC+CGG 0.607659 1.1:+58397303 MS.gene86827:CDS
CAATCACCTTTAGATTAAGT+GGG 0.608336 1.1:-58392061 None:intergenic
TGAATGCACACCAGAATGAG+AGG 0.609087 1.1:-58393466 None:intergenic
AAAGGTTATACACATAGGAT+GGG 0.610075 1.1:-58397928 None:intergenic
TTACAATTGTCTAGGTACAA+AGG 0.610376 1.1:+58391972 MS.gene86827:intron
ATGTTCACTGAACAGTGGGA+AGG 0.611843 1.1:+58397081 MS.gene86827:intron
CGGGTGACAGTGGAAGGAAG+TGG 0.612301 1.1:-58395907 None:intergenic
GACAGTCACAGTGTACGGCA+TGG 0.613993 1.1:-58395974 None:intergenic
TGCATAAAGGTTATACACAT+AGG 0.622320 1.1:-58397933 None:intergenic
TCAAATCCAGATTGTGAATG+GGG 0.623330 1.1:-58397880 None:intergenic
GTGGCCTATCGACTCAAGAG+TGG 0.623354 1.1:+58396258 MS.gene86827:CDS
AAGGTGGACCAGCATTGCCA+AGG 0.624449 1.1:+58391991 MS.gene86827:CDS
ACACTAGTTTGTCCTGAATG+TGG 0.624818 1.1:+58395841 MS.gene86827:CDS
ACAGTGGGAAGGGTGGAGAG+AGG 0.626561 1.1:+58397092 MS.gene86827:intron
CACATGTCATCTGGTCCTAG+AGG 0.629509 1.1:-58397616 None:intergenic
GGAAGAGAAACGGATCGTGA+AGG 0.630973 1.1:+58388576 MS.gene86827:CDS
CTTCAACTAAGTCTCATGAA+GGG 0.631995 1.1:+58397224 MS.gene86827:CDS
ATCATCTGTATCAATTGCAG+GGG 0.637469 1.1:+58393530 MS.gene86827:CDS
ACCGAAGCAGTACAGCATTG+AGG 0.637486 1.1:+58392034 MS.gene86827:CDS
TGGTTATAACCACTGAATTG+TGG 0.641543 1.1:-58394051 None:intergenic
GAAACTGGTTTCATCTCAGG+TGG 0.641699 1.1:+58397250 MS.gene86827:CDS
TCACTGAACAGTGGGAAGGG+TGG 0.648017 1.1:+58397085 MS.gene86827:intron
TCTTCCAAATAAGTAACCAA+GGG 0.649627 1.1:-58397130 None:intergenic
CTCTAGGACCAGATGACATG+TGG 0.653845 1.1:+58397617 MS.gene86827:CDS
GTCTATAGTGGTGAAAGAGG+TGG 0.657063 1.1:+58393654 MS.gene86827:CDS
ATTGAAATGAAGGACTCGGA+TGG 0.658538 1.1:+58395462 MS.gene86827:CDS
TAAAGGAGTAGAACAGCACA+AGG 0.658863 1.1:+58392899 MS.gene86827:CDS
GCAATCAGCAACCATTACGG+TGG 0.659658 1.1:+58397953 MS.gene86827:CDS
TAGCTCATAGAGTTCACTCA+AGG 0.664124 1.1:-58392865 None:intergenic
CAATTGTCTAGGTACAAAGG+TGG 0.670376 1.1:+58391975 MS.gene86827:intron
TCAGAATTATCAATTGGACC+GGG 0.676607 1.1:-58391527 None:intergenic
TGCAACAGCAGTTCGCCCAG+AGG 0.677930 1.1:-58393992 None:intergenic
CTAATTCTCACAGTGAACCG+AGG 0.686154 1.1:+58397296 MS.gene86827:CDS
TTCTTGACCGAAGAACCTCT+AGG 0.686595 1.1:+58397601 MS.gene86827:CDS
CTTTGCACCAGAGAACAAGA+GGG 0.690692 1.1:+58397328 MS.gene86827:CDS
GAGTGACCATTAGACAAGGT+AGG 0.713316 1.1:-58393555 None:intergenic
TTACAGCGATGAGATCAACA+TGG 0.715649 1.1:+58393776 MS.gene86827:CDS
TCATCTGTATCAATTGCAGG+GGG 0.722106 1.1:+58393531 MS.gene86827:CDS
GTGAATGGGGAAGTTCACAA+AGG 0.723784 1.1:-58397867 None:intergenic
TTGAAGATAACTGATAGACC+CGG 0.726861 1.1:+58391509 MS.gene86827:CDS
TTTGCACCAGAGAACAAGAG+GGG 0.735618 1.1:+58397329 MS.gene86827:CDS
ACTTGAGTGACCATTAGACA+AGG 0.740534 1.1:-58393559 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.1 gene 58388511 58398301 58388511 ID=MS.gene86827
chr1.1 mRNA 58388511 58398301 58388511 ID=MS.gene86827.t1;Parent=MS.gene86827
chr1.1 exon 58388511 58388662 58388511 ID=MS.gene86827.t1.exon1;Parent=MS.gene86827.t1
chr1.1 CDS 58388511 58388662 58388511 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58391412 58391666 58391412 ID=MS.gene86827.t1.exon2;Parent=MS.gene86827.t1
chr1.1 CDS 58391412 58391666 58391412 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58391986 58392127 58391986 ID=MS.gene86827.t1.exon3;Parent=MS.gene86827.t1
chr1.1 CDS 58391986 58392127 58391986 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58392861 58392920 58392861 ID=MS.gene86827.t1.exon4;Parent=MS.gene86827.t1
chr1.1 CDS 58392861 58392920 58392861 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58393021 58393116 58393021 ID=MS.gene86827.t1.exon5;Parent=MS.gene86827.t1
chr1.1 CDS 58393021 58393116 58393021 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58393432 58393818 58393432 ID=MS.gene86827.t1.exon6;Parent=MS.gene86827.t1
chr1.1 CDS 58393432 58393818 58393432 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58393946 58394086 58393946 ID=MS.gene86827.t1.exon7;Parent=MS.gene86827.t1
chr1.1 CDS 58393946 58394086 58393946 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58395396 58395566 58395396 ID=MS.gene86827.t1.exon8;Parent=MS.gene86827.t1
chr1.1 CDS 58395396 58395566 58395396 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58395826 58396083 58395826 ID=MS.gene86827.t1.exon9;Parent=MS.gene86827.t1
chr1.1 CDS 58395826 58396083 58395826 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58396180 58396325 58396180 ID=MS.gene86827.t1.exon10;Parent=MS.gene86827.t1
chr1.1 CDS 58396180 58396325 58396180 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58397096 58397638 58397096 ID=MS.gene86827.t1.exon11;Parent=MS.gene86827.t1
chr1.1 CDS 58397096 58397638 58397096 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58397739 58398009 58397739 ID=MS.gene86827.t1.exon12;Parent=MS.gene86827.t1
chr1.1 CDS 58397739 58398009 58397739 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
chr1.1 exon 58398158 58398301 58398158 ID=MS.gene86827.t1.exon13;Parent=MS.gene86827.t1
chr1.1 CDS 58398158 58398301 58398158 ID=cds.MS.gene86827.t1;Parent=MS.gene86827.t1
Gene Sequence

>MS.gene86827

ATGACGATTGCTGAATCGGATTTTCCGATTGATAATGGGATGAGTTGTGTGCCGATTTCACCGGAGGAAGAGAAACGGATCGTGAAGGAGTTGATAAAGCAATCGGAGCTTAATTTGAAAGATGGGAACTTATACTTTATTATTTCCAATAGGTTTTAATCTTATCTTAATCTTGTTATATGATTTTGAATTTTAGGGATTTTATGATATATGCATGATGAATTCAATTAATTATATTGCTATATGCATGTGGATATTGTATTACTTGTGCTGTTTGTTTTGATATGTTATTTTTAGGGTTTCTCGAATATCGGTTATCTGAGCCCTTTTTAAAATTTTATTAGGGGGATTTTGTTGATCATTTGTCGTTTTTGGGCTTTGTCGTAGATGTTTGATGATTGTTGCCAAGCAATAAATTTGTATCCCGAAAACGGCTAGTCTAGCCCCGTTTAAAATCTTATTAAGGAGTTGTTTTTTTTTATCTGTTAAGGTAATGTTACTTTACCTTAAGTGAGTTGTCGACAATCAATAAATTTGCCTCCCGATAAACAGCCTGTCTAGCCCTTTTTAAAATCTAATGAGAGAATTGCTTCATTATCCGTGAAGATAATGTTCCTCTACCTGCGATGAGTTGTTGATCCTTTGTCGTTTTCCAGTTTTGTCGTTGATGTTTGATGATTATTGGCAAGCAGTAAATTTACCTCCGATAAACGGCTAGTCTAGCCCCATTTAAAATCTTCTTGGTTTTTTTATCTGTTAATATTATGTTTCTTTACCTATGTGAGTTGTTGATCATTTGTCGTTTTCCGGTTTTGTCATAAGTGTTAAATGATCAATGACAACAATAAATGCTTGGAGACAGACATTGTCTACCGTATTCTCATTCCTATTACTGTTATCGTTCACAAAATAGTTTGTGGCGTGGCCGGCATAGAGTTGCAATGAATGTCTGGTCGTGAAATCGCGGCATTATGTCGCATTTTTATAGAGCAACGATGGCAGCGTGAATTGCAGGGAAATGATGGACTAATCGAGGCCAACACGGTTAACAAAAAATTTAATCCCCGTTATCTGTCTCTTTTGTTCATTAACCCTTAATTTGTCAATGATTTTTGACCTGTTTCTCCATTGGAATGAAGATACAGTTTGAGACCCTTCATTATTCCAAGAATGATCCACTAGCTTGCAAATCTGTTTTTTCACCTCCTCCATCTCCAGCAGGTGATGCATTTCCTGGCTAATCCCTTGGTATACCTCAGCCTCTGCATCATAATTAATAATCTTAGACTGCATAGAGTCAATACCCAACCTAGAAAATGCTTTAGCAGGATGTCTTTTGTTTTGAAGATTAGAATAGAGACTAAATAATTTATACTGCTCACAGTAAAAAAAAAAAACCAAAATTAAAAGCTTTTTATTAAGTAATTATGTCCTTGTCGATATAGGAGCATGGGGGATGGGTAGCTCAATTGGTTGAGCTAAGGGATTAAAGGAGTTAAGGAGTGAAAGGTCCGTAGTTTGAATCCTCGAAAGGGAGAAAATGCTAACATAACAATTTACATACTAACATTTGCTGTTCCAAAAAAATAAACTATCCATATAAGAGCAATGAGAACATAGGAAAGAAATAAAAGATTTGCTGGAATTAATAGGAAAAAGCAGAACAAGAACAAATTAGATAAAAATTAGAAGTATGTGAACTGTGAAGTGTAAATAGAGACCGAACGCTGATGCATCAACATAGACCAAGACCAACCAAAGAATGGATATCTTTCAGCAGAGGGTCAGTGGTCCAAAAATGTTGATGGCCGGAGAAGATGATGGGGAGCAGCGAGTCACTGAGGTCAAACAGCGTTGTTCAAGACAGAGGTGTTTTTGTGTGTGTGTGTGACTGATAAAGGAAGAAGGATTTCAACCCCAATAACTTTTCTGAAGCAATGTACTGCACGGATAATTACGAGTCCGAAGAAAATCAGATATCCAGGGAAAATGTTGGTGTTTAGCCCCTGTTCACCACAGCAGCCTCAATGATTGCATTTTTTTGCATCAGTGACTGTCATCTTGAGCAAGATGGCGAATGCAACCCAATAAGAAACGGAAATAAAAATGTTGTGGTCCCCTATCATGTGATGCGACAGAGACAGCACTACTGCGTCTCTATTGTGCTTGATTGAACTCCAACACAATTTTTTGCCAGCCCCTGAACCTCTTCTGAGTCTCTTCTTTCTTCTGGTTTGTGTCCATAGTACTGACAGGCCTGCTCTTTGTTTTTTTACTGTAATTTTTTCCTAGGTTCTTTCTCATGTTTCTTTTTACTTTGAGACACAGAGACACATCTTCTGTGGCTCTTTCGAAGAGAAGGCGTAATTATCAGCGATCATATATCTTTCATTTTGGGTATTTTTTTATTTCAAATGTTTAACTGTGTGGTATGAATTACCCCCTTTAAATTTGGTACAGCATCCATTTCGCTATCTGCAATCCTACTAATTTGTTTTTTGGGTCAGCTACTTTGCTATGTGGGTTTGACGACTATAATCTTTCGTTAAGTTGCACAATGTTTTATATTTTTTTCCCCAATATATAATTTGTGATGTACCATACAATCTATTAATTAAACACATATCTGTGCCGCTTTTTGTTACTCTGATCTTGCAATAAATCACGTTGACCTTTAACAGTGGTTATATGGGCTCATTTGTTCAAGTTTAAGGAAAAACCACTTCTTTACGTCAAGAATATGCCTAAGTTCTTAAGATGTTTAGATAAGTTTTAGTGATTTTTAGAAAGTCAAAATCAAGTACAAAAGTACAAAAGCTTATCAAAATGTTCCCTATATTGTATTACATTTTATATGTAGCCTGCCATTTTTAATCAAGACTTTGCTTACCACCCATGCAGATGGTTTTCAAGTTGGCAGAGGTATGTTGGGCATTGTGATGGAATACTTTCTACTGACCAGCAATCTTCTGATGGCCTGCATGCCGATACTGTTCACTTGAAGATAACTGATAGACCCGGTCCAATTGATAATTCTGATATAATTTCAAATGGAAGTAATTGTGACGGGAGTGATTTAGATATTCATCGACCATTGGAAGAGGATAAAGACTATGTTTTAGTTCCTCAACAAGTATGGGAAAGGCTGTTGGGATGGTAAGCTTATTATTCTCAATACTTTTTTTGCTTTTTGGTCTGGAAACTGTTAATAATCTTGTGGTTTGTAGTTGTAAATAATACTGTAAGAGTTAAATAAGCATATATTATCTTTTGATGCTTCAACCGTTCTTTTAAAAAAGAGCCGCAAACCATCTTTTAACACGTTTATTTAAGTATGCAGTGGGATGTTTTCGTAGAGACGTCACACAGATTTAGTTCCACAAACTAAGTTTCAGGAATCAACTTAATGAAGCAGAAGCCTAAAATGCTTGATATTTGTAGTGTGTGAACTGATTTTTATCTTACAATTGTCTAGGTACAAAGGTGGACCAGCATTGCCAAGGAAGTTGATTTCTCAGGGTGTACCGAAGCAGTACAGCATTGAGGTGTACCCACTTAATCTAAAGGTGATTGATGCGAGAGACAACAGCGTTTCAATTGTAAAATTAAGCAAAAAGGTACTGATTGATTTCTTTTCAACCTCTTTTCTTGTAGTTAGTTTTCTGGTAATGCCAATGATTAGAAAAGGGTTTTGGTGGTGTATCTATTTGTATGGAGCATGCTCTGTATGGTTGGTGTTTGTTTGATATCACTATAGGAAATGATTATACTATTTAGTTTGTATGAATGTTAGTAATTTAACATGACTAAATAATGTTATGAAAGAAGCAATTTTTATATTCTGTATGTTTATACAAGATCATCGTAAAGATTAGTATTTCGCTTGGAATAAGTTGAATTAAAAACTGACATGTTTTTATGTTTAATGAATGCCCAATTAACCATAAAAATTTAAAATTGAATTATATGTGTGCGTGCGCGTGCATATGTATATTGGCATCCTTACATATATGTGCTAGTCAATCTGAGCTCTCATTTCATTTAGAATGTAGAGAGAAAAATAATAGTTAAAATCTCTACGAGAACTTTGGGGTTTGGGCAATAATTTATCTTGCCACGAAATTACATAATTTATAACAAGTTATCAACAACATATTCTATTATAAGACATTTATTGATGGGCATTAGTTTTTTCCCAGAAGATTACAAATATAGGAATTTTACTCTTTTTTTTTTTTTTTATCTGGATTAGAATAGCATCAAATTTGGTTATAACATGATATTTATGATTGAAATGTAAGATGAACCATCCTTATGATTTTTGTTTTCTATTCCTGTATTGTATTTTAGGCTACCTTGAGTGAACTCTATGAGCTAGTGTGCAAAGTTAAAGGAGTAGAACAGCACAAGGTGGTATTCTTTTTTATGTTTTCTTTTTCTTTTTCATTGTTGTAAATCTCAGACTTCTGATTCCATCGTTTTGCATTTTATTGGTTGTAAATTATATTAGGCTTCTATTTGGGATTACTACAATTTGAACAAAGAGTCATTGCTGACGGCTTCAGACCAGACATTGGAGAATGCAAACTTGGCAATGGACCAAGACGTGAGTAACTCTGAATTGTAAACATTTTTTTTTATTGATTGATTGATTTGAAGCTGCTAATAGAAGTACAAAGCTTTAATGGGACCTTGAGCCTACAAAGTGGATATTTTTAAGTTACTATAATTATGATTTTCCATTAGAAGGACGTTTATGTCCAATATATGATATTTTGAGTGTCTTTAAGTTGAGCGAGGTGCTGAATATTCTTATGGTATGATTTGATTTGATGTTTAGCTATTATGATATCCTAATTCTTTGCTTGGTATTGATTCTTCTATAGAAGCTTGATGCCCTTAATTTATCTCTTGCTCACAGATTCTTCTAGAAGTTTCAGTCGATGCTGATCGTTCCTCTCATTCTGGTGTGCATTCAATGGGAAATGAATTAGCATTGGTACCATTAGAACCTCCAAGATCATCTGTATCAATTGCAGGGGGACCTACCTTGTCTAATGGTCACTCAAGTGGGTCTGGTTTTAATTTCTATCAGGGAAGTTCTGTAAGTTCATCACTGACTACTATGGATGATAAATATGACGTCTATAGTGGTGAAAGAGGTGGTTTAGCAGGACTGCAGAACTTGGGGAATACCTGCTTTATGAATAGCGCTCTTCAATGTTTGGTCCACACTCCACCACTTGCTGAATTTTTCTTAGAAGATTACAGCGATGAGATCAACATGGAGAATCCTTTAGGAATGCGTGTATGGACCATTTCTGTTGGCTTTTATTTTTTCTCCTGGTGTTCTCGGATGCATAAGATATTGTGTACCACAATGGCTTTACTTTCAAGGCAATTTAAATTAATTTCAACATTTCTCTGATCTGCAGGGTGAATTAGCACTTGCATTTGGGGACTTGTTGAGGAAACTTTGGTCCTCTGGGCGAACTGCTGTTGCACCACGTGCGTTCAAGGGAAAGCTGGCCCGATTTGCTCCACAATTCAGTGGTTATAACCAGCATGATTCTCAAGTTAGTTTCTGCATTTTGTACAATTTTTATTTTTGGAATGTCAAGTAAATAAACCCTAGCGCTTTTATTACCTATGACAAAAACTTCCTGTTACACGATGCCTGTTGGTCATTAGCATTTTCAATCATATGATTTTGATTTTTGGAATGTCGTCAAGTAAATAAACCCTAGCGCTTTTATTACCTATGACAAAAACTTCCTGTTACACGATGCCTGTTGGTCATTAGCATTTTCAATCATATGTTATGAATAAGGAACAAATGCATGCAATTATTTTTTATATTAAGTTACAGTTTGGGCCAATGAAATATGAAATAATTCTGTAGTTCTTTTTTTTTTTTTTTTTTATAATTCTTTATAGTTTTGTTTTGATATATATATATATATATATTTCATTTTTTAAATGGTCTAACAGTAATTGTAACTAGCAATTCTTTGTTAAAAATTGGCTGCCAAAAGGTTCTATCTGTGACTTGACATTTTCTGTAGTTTTCAAGAACTCGTCTAAAGGATCATTCATTTTTCAGGGATATTCAAACTGTATTTTAACCTTATTTTAGTTGGGTTCCAGTTTTACAGTAGCAAGAGCTTTAAAAATTGGCATTCACTGCATTCTGTCATTTTCATATTACACAGTATAGACATTTTATACCCATCTGGTTTCTTTCATAACATGTTAACAATTGTTGATTCTGGTGATTAGCATTTTTATGAGAAAATTACTATGTTGTGCCATGCGGTTTTTGTTTTAAATTAGGAGATTACAATCATGGGTTTGTAATTTTGTAGGATCTTTTATTAGACTCATTGAAGTTGTCTGTTGCCAGTATGGTATACCTGTATGGCCGACGTTCATCAGTTGTGATCTAGATCTGAATGTTATTTTATGTATGTGTGTTTGTAGAAGTAGATATGTTTTTTGTGATGTTAATTTTTATCCTTTTTACTGCTCTAAGAACTAAAATGGTAGCGGAAAATTTAATCCCAAAAAATTTATTCATTGATCTCTGTTGTAGTTTTCTTCTTTCTGACTTTCCATTTGAGTTAATCCTAATTTGTATTACGTATACATCTTAGTTTATTAAAGGACTGGAGAATTGGGTATCTTTTGCTTAACCTGCATTTGATATAATATAACAAGTTTGCGTTAGGCCAATGTCGGACTTGGAAAATATGACTGTTGCTTGATTTCTTACTCGAGTCTTGACAACTTTATGGTTGTTTTAAAATGACCTTTCCTACTAATTTCTGAAAGCTATTGAACTTCTTTTTCTCGCGTGTTCTCTGAGTAGTGAGTTATTTTTTTTAGGAATTGCTAGCCTTTTTATTGGATGGACTGCATGAAGATTTGAATCGTGTGAAACAAAAGCCTTACATTGAAATGAAGGACTCGGATGGTAGGCCGGATGAGGAAGTCGCAAATGAGTGTTGGAAAAATCATATGGCTCGGAATGATTCATTGATTGTTGATGAATGCCAGGTGAGTACTTACAGAAAAGTTCTTGTTTCGCAGTCACATGTCACATGAATTCCAGTTGATCTTCCATTGATAAGCTGAGTAGCTACATGTTTTGTTGAGTTTTCTTCCCCATCTCAATAAACCTTCATTGATTTCTATCAATTCCTGAGCTTGGGTTTCCATTGAAGCTTTGACTAGAGTTTCAATTGAAGTACATGCGTACATGCTCGTTATCTTTACTAAGCTTGACATTTTGATTTTTCTTGAATAAAATGCTCAGGGTCAATACAAATCGACACTAGTTTGTCCTGAATGTGGCAAAATTTCAATCACTTTCGATCCCTTTATGTACTTATCATTACCACTTCCTTCCACTGTCACCCGGACAATGACAATTACTGTCTTTTATTCTGATGGGAGCGGTCTTCCCATGCCGTACACTGTGACTGTCCTGAAGCATGGTTGCTGCAGAGATCTTTGCCAAGCATTGGGCACTGCATGCTGTTTGAAAAGTGATGAAATGCTTTTTCTTGCAGAGGCAAGTGCTTTTCAGAAAAGTTAGATCTACTCTTGGCTTTTCTACATGACTTTGGTTTTATTTCATCATTAATGTTTTATGCTGTCTATATCTCAGGTTTATGAGCATAAGATTTATCGTTATCTAGATAATCCATTGGAATCATTGACTTCAATAAAGGATGAAGAACATATTGTGGCCTATCGACTCAAGAGTGGAGCCAGGAAAACAAAATTAGAAATATTGCATCGACATCTGGATACGTAAGTATACCTCTTTCAGTGTATACTCTTAAGTTGTGAAATGCACAAAGCTCTGTTGAGTAATGGTTTCTATATACTATGATTCTATGATAGTTTTTATTGCCTGAGTGACATTACAGTGAATATGGGTCAAAAAATTATTGATAAAGTTATTCTGAATTCTGTTGTAGTTGTAATAAACTTTGGTTAAATTGATCTTTCCTACTTGAAAGCAATTGACTGGAGACTGTTATATTTTGGTGTGAACAGTTTCATTTATCCATTGAACTCTTCCAGAGTACAAATGGGAATAAATCAATCAGACATTGATAATGGTGGTTAATGCCACCTGTCTGAACACATAAACTCTTCAAGGCAATCATTTTCTTATCACTTGTTTATAACCCATAATGATAAAACATTATGGCATCATTTTGTCCATGTAGCCGACCCCGCCTAGTGAGATATGGATGTGTTTGTAACCCAAAACACTAGACCATAACTCCCATCAGTTTTCTGATTGTTTGGTAATTTTCTCCACTATTGGTCATTTCAAGTACTACTGAATTTTGCAGGTACCATTCAAGTTGGTACCTATATGTGGTATAGCCGTATAGCATAGTTTGCACAACCTGGCTTAGCATATAGAAGTTTACCATAGAACGATTCCACAACCAGTTATTTATATTTCACTGATTGTACACGGTGCATTGACTTACAACATTATAATACTTTAATGTCTCTAACACTGGTAATTTGATGTGTGAATTATCTTCATGTTCACTGAACAGTGGGAAGGGTGGAGAGAGGAAGATCTTTGGAACTCCCTTGGTTACTTATTTGGAAGAAGACCCTCAATATGGAGCCAACATTGAGACATATGTACATAGAATGTTGGCACCTTTAAGAAAAGCACATTCTTCAACTAAGTCTCATGAAGGGAAGGAAACTGGTTTCATCTCAGGTGGTAGTGACGAGCAATCAAGCATCTCTAATTCTCACAGTGAACCGAGGAACCCGGCACTTTGCACCAGAGAACAAGAGGGGACATCCTGTGGAGAGTCATCTTTCCAACTTGTTTTAACTAACGAAGGCTGTTTTAGCTGTGAGCCCATTGACAAGGCATCTGTGATAAAACCCAGTGCACATATAAAGGTCTTTGTTGATTGGACTGAGAAAGAATATGAATTATATGATGCTAGCTATTTGAGGGATCTTCCTGAAGTTCACAAAACTGGATTCACTGTGAAAAAAACCCGGCAAGAAGCTATTTCTTTGTTTTCATGCTTGGAGGCATTCTTGACCGAAGAACCTCTAGGACCAGATGACATGTGGTAAGATTGCGTTGATGTTTTTGGAATGGATTTCATAGTTGATTACCTTGTACGCATTGATTTTTGTTTTAATTTATTTTCTTTTTAAAAAATTAATTAGGTACTGCCCTCAATGCAAGGAACACAGACAAGCTACCAAGAAACTGGACTTGTGGAAATTGCCAGAGATTTTGGTTTTTCACTTGAAACGATTCTCATATAGCAGATACTTGAAAAACAAACTTGACACCTTTGTGAACTTCCCCATTCACAATCTGGATTTGACCAAGTATGTGAAAAGTAAAGATGGCCCATCCTATGTGTATAACCTTTATGCAATCAGCAACCATTACGGTGGTTTAGGTGGTGGGCATTACACTGCATATGCTAAGGTAAGTTAGTGACATGCATAACAATTATACTGCTTGATTTTTCATGTTCACTGTGTGGTCATGCCAAATCTAGATTTTGTAATTTGTGAATTCTTTGTGCTGCGTGAGATGCTTCTGTTTATAACCTTATGTTATTTGTTCTGGGCAGTTGATTGATGATAATAAGTGGTACCATTTCGATGACAGTCACGTCAGTCCTGTTTCTGAAGCTGAAATTAAATCTTCAGCTGCATATGTGTTATTTTATCAGAGAGTTGGAAGCGACGTCCAGATGGAAGAAGCGACGTCTTAG
Protein sequence

>MS.gene86827.t1

MTIAESDFPIDNGMSCVPISPEEEKRIVKELIKQSELNLKDGNLYFIISNRWFSSWQRYVGHCDGILSTDQQSSDGLHADTVHLKITDRPGPIDNSDIISNGSNCDGSDLDIHRPLEEDKDYVLVPQQVWERLLGWYKGGPALPRKLISQGVPKQYSIEVYPLNLKVIDARDNSVSIVKLSKKATLSELYELVCKVKGVEQHKASIWDYYNLNKESLLTASDQTLENANLAMDQDILLEVSVDADRSSHSGVHSMGNELALVPLEPPRSSVSIAGGPTLSNGHSSGSGFNFYQGSSVSSSLTTMDDKYDVYSGERGGLAGLQNLGNTCFMNSALQCLVHTPPLAEFFLEDYSDEINMENPLGMRGELALAFGDLLRKLWSSGRTAVAPRAFKGKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNRVKQKPYIEMKDSDGRPDEEVANECWKNHMARNDSLIVDECQGQYKSTLVCPECGKISITFDPFMYLSLPLPSTVTRTMTITVFYSDGSGLPMPYTVTVLKHGCCRDLCQALGTACCLKSDEMLFLAEVYEHKIYRYLDNPLESLTSIKDEEHIVAYRLKSGARKTKLEILHRHLDTGKGGERKIFGTPLVTYLEEDPQYGANIETYVHRMLAPLRKAHSSTKSHEGKETGFISGGSDEQSSISNSHSEPRNPALCTREQEGTSCGESSFQLVLTNEGCFSCEPIDKASVIKPSAHIKVFVDWTEKEYELYDASYLRDLPEVHKTGFTVKKTRQEAISLFSCLEAFLTEEPLGPDDMWYCPQCKEHRQATKKLDLWKLPEILVFHLKRFSYSRYLKNKLDTFVNFPIHNLDLTKYVKSKDGPSYVYNLYAISNHYGGLGGGHYTAYAKLIDDNKWYHFDDSHVSPVSEAEIKSSAAYVLFYQRVGSDVQMEEATS