Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89597.t1 | XP_024629315.1 | 91.8 | 462 | 38 | 0 | 1 | 462 | 1 | 462 | 1.10E-236 | 828.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89597.t1 | Q557M8 | 24.9 | 389 | 212 | 10 | 8 | 377 | 14 | 341 | 9.3e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89597.t1 | G7LDX0 | 87.9 | 479 | 41 | 2 | 1 | 462 | 1 | 479 | 1.3e-229 | 805.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031871 | MS.gene89597 | PPI |
| MS.gene052488 | MS.gene89597 | PPI |
| MS.gene89597 | MS.gene007951 | PPI |
| MS.gene89597 | MS.gene39443 | PPI |
| MS.gene89597 | MS.gene08242 | PPI |
| MS.gene007950 | MS.gene89597 | PPI |
| MS.gene31944 | MS.gene89597 | PPI |
| MS.gene89597 | MS.gene33782 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89597.t1 | MTR_8g077000 | 87.891 | 479 | 41 | 2 | 1 | 462 | 1 | 479 | 0.0 | 798 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89597.t1 | AT1G03280 | 58.134 | 461 | 162 | 12 | 1 | 444 | 12 | 458 | 2.71e-173 | 495 |
| MS.gene89597.t1 | AT1G03280 | 58.134 | 461 | 162 | 12 | 1 | 444 | 12 | 458 | 2.71e-173 | 495 |
| MS.gene89597.t1 | AT1G03280 | 58.134 | 461 | 162 | 12 | 1 | 444 | 12 | 458 | 2.71e-173 | 495 |
| MS.gene89597.t1 | AT4G20340 | 52.203 | 454 | 194 | 9 | 1 | 444 | 13 | 453 | 9.23e-141 | 412 |
| MS.gene89597.t1 | AT4G20340 | 52.203 | 454 | 194 | 9 | 1 | 444 | 13 | 453 | 9.23e-141 | 412 |
| MS.gene89597.t1 | AT4G20810 | 47.966 | 467 | 178 | 14 | 8 | 461 | 1 | 415 | 5.57e-132 | 387 |
| MS.gene89597.t1 | AT4G20340 | 52.206 | 408 | 173 | 8 | 47 | 444 | 3 | 398 | 1.30e-122 | 364 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.3:-27140037 | None:intergenic |
| GACTTGCCTGTTCCAGAATT+TGG | 0.170288 | 8.3:+27144189 | MS.gene89597:CDS |
| AACTGACAGTACATCTTTAA+AGG | 0.175603 | 8.3:+27145575 | MS.gene89597:CDS |
| CGACCGATTTGGTCTATTAA+AGG | 0.179666 | 8.3:-27144159 | None:intergenic |
| GCTTGAAGACTGCCAAATTC+TGG | 0.275198 | 8.3:-27144201 | None:intergenic |
| ACGACGGCGCGAAAAGTTAA+AGG | 0.307914 | 8.3:+27142367 | MS.gene89597:CDS |
| ATCTGCTGTGTTCACATCTT+GGG | 0.327954 | 8.3:-27145968 | None:intergenic |
| CATCTGCTGTGTTCACATCT+TGG | 0.328566 | 8.3:-27145969 | None:intergenic |
| TAATGCTGGTGATTCTAATC+TGG | 0.336277 | 8.3:+27144269 | MS.gene89597:CDS |
| GAAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.3:+27140024 | MS.gene89597:CDS |
| GTGAAATTCGTACTTATTGT+AGG | 0.350382 | 8.3:-27139814 | None:intergenic |
| GGGCTGATTATCTTCTTTAC+CGG | 0.354315 | 8.3:-27140016 | None:intergenic |
| GCATAAGCTGAAAGATGAAT+TGG | 0.358679 | 8.3:+27142049 | MS.gene89597:CDS |
| GATTCTAATCTGGGATATAA+TGG | 0.362821 | 8.3:+27144279 | MS.gene89597:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.376308 | 8.3:+27146066 | MS.gene89597:CDS |
| TCATCTTCATTGTCTTCTGC+TGG | 0.387140 | 8.3:-27151628 | None:intergenic |
| ACTTAAGAAGCAACACGAAT+TGG | 0.389424 | 8.3:+27145927 | MS.gene89597:CDS |
| TATGTACAAATTGTGGAAAA+AGG | 0.405813 | 8.3:+27142099 | MS.gene89597:CDS |
| GAATAAAATGTACCTCTAAC+AGG | 0.409316 | 8.3:-27146078 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.3:+27144243 | MS.gene89597:CDS |
| GAAGCTCGAGCTAGTGCAGC+TGG | 0.419733 | 8.3:+27144228 | MS.gene89597:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.3:-27140926 | None:intergenic |
| TGCAGATATATAAAGGGGTT+AGG | 0.445590 | 8.3:+27142006 | MS.gene89597:intron |
| TTCCATTGTGAATGTTGTAA+TGG | 0.457370 | 8.3:+27142275 | MS.gene89597:CDS |
| TCGACTGGGAAGAAGGTTGA+TGG | 0.459603 | 8.3:+27151653 | MS.gene89597:CDS |
| TTTGTAGGATGAGTATATCA+AGG | 0.460980 | 8.3:+27145888 | MS.gene89597:intron |
| CGATTGGAAGTACTGCTAGA+AGG | 0.461514 | 8.3:-27145994 | None:intergenic |
| AGGAAAAGATGGAGAAGAAA+AGG | 0.464213 | 8.3:+27141774 | MS.gene89597:CDS |
| ATTGTCTTCTGCTGGAGCAT+CGG | 0.466214 | 8.3:-27151620 | None:intergenic |
| ACAGCGCGGAGAGGTGAAGC+AGG | 0.479424 | 8.3:+27145644 | MS.gene89597:CDS |
| AGACTGCCAAATTCTGGAAC+AGG | 0.481046 | 8.3:-27144195 | None:intergenic |
| AGATTATCACTAGAGTTCAT+AGG | 0.486428 | 8.3:+27140982 | MS.gene89597:CDS |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.489229 | 8.3:-27144312 | None:intergenic |
| AAAGGACATGCTTCAAAAGA+TGG | 0.489353 | 8.3:+27142385 | MS.gene89597:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.3:+27145603 | MS.gene89597:CDS |
| AATGCTGGTGATTCTAATCT+GGG | 0.496431 | 8.3:+27144270 | MS.gene89597:CDS |
| GAATATATATGTACAAATTG+TGG | 0.502239 | 8.3:+27142092 | MS.gene89597:CDS |
| TTTCATGCCAACTTGGCGAT+TGG | 0.507113 | 8.3:-27146010 | None:intergenic |
| TGAAATTCGTACTTATTGTA+GGG | 0.520023 | 8.3:-27139813 | None:intergenic |
| ACAATGAAGATGATGTCGAC+TGG | 0.524851 | 8.3:+27151638 | MS.gene89597:CDS |
| GCTTTCTATGATGATTATAC+CGG | 0.526519 | 8.3:+27139997 | MS.gene89597:CDS |
| TCATCCTTGATGCCCTCACC+AGG | 0.539237 | 8.3:+27140076 | MS.gene89597:CDS |
| AGGAAATGGAGGTTACAAGA+TGG | 0.544484 | 8.3:+27151582 | MS.gene89597:intron |
| CACTAGAGTTCATAGGAAAG+AGG | 0.545956 | 8.3:+27140989 | MS.gene89597:CDS |
| AGTACTTCCAATCGCCAAGT+TGG | 0.547271 | 8.3:+27146003 | MS.gene89597:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.3:-27145606 | None:intergenic |
| GATGCTACGAAAGGAAAAGA+TGG | 0.554983 | 8.3:+27141763 | MS.gene89597:CDS |
| ATTTGGCAGTCTTCAAGCAT+GGG | 0.558972 | 8.3:+27144206 | MS.gene89597:CDS |
| TGAACCTGGTGAGGGCATCA+AGG | 0.563106 | 8.3:-27140080 | None:intergenic |
| CAGGTGGTAGTTGACTTCAA+TGG | 0.563361 | 8.3:+27145519 | MS.gene89597:intron |
| AATTTGGCAGTCTTCAAGCA+TGG | 0.564482 | 8.3:+27144205 | MS.gene89597:CDS |
| AAGCCTTTAATAGACCAAAT+CGG | 0.565907 | 8.3:+27144156 | MS.gene89597:CDS |
| GATGATGTCGACTGGGAAGA+AGG | 0.566390 | 8.3:+27151646 | MS.gene89597:CDS |
| TTTAATAGACCAAATCGGTC+GGG | 0.566690 | 8.3:+27144161 | MS.gene89597:CDS |
| CAATGAAGATGATGTCGACT+GGG | 0.567076 | 8.3:+27151639 | MS.gene89597:CDS |
| TTGATAAATTGAACCTGGTG+AGG | 0.571718 | 8.3:-27140089 | None:intergenic |
| GTATCTCCGCTGTATGGCAG+TGG | 0.571785 | 8.3:-27144306 | None:intergenic |
| AAGCTCGAGCTAGTGCAGCT+GGG | 0.572134 | 8.3:+27144229 | MS.gene89597:CDS |
| GCTGCTCAAGAAGGTGGAGA+TGG | 0.572571 | 8.3:+27142326 | MS.gene89597:CDS |
| AAGTTAGCTGCTCAAGAAGG+TGG | 0.576893 | 8.3:+27142320 | MS.gene89597:CDS |
| ATCAGCTGTAGCAGCTACAG+CGG | 0.579271 | 8.3:-27141743 | None:intergenic |
| AAACGCAAGGAAGATGATGA+TGG | 0.583208 | 8.3:+27146036 | MS.gene89597:CDS |
| TGATAAATTGAACCTGGTGA+GGG | 0.583295 | 8.3:-27140088 | None:intergenic |
| TCTGCTGTGTTCACATCTTG+GGG | 0.584121 | 8.3:-27145967 | None:intergenic |
| TGTACATATATATTCTCGAA+CGG | 0.588463 | 8.3:-27142084 | None:intergenic |
| GTGTTGCTTCTTAAGTAAAG+CGG | 0.592790 | 8.3:-27145920 | None:intergenic |
| GAAAAGTTAGCTGCTCAAGA+AGG | 0.594454 | 8.3:+27142317 | MS.gene89597:CDS |
| CTTTAATAGACCAAATCGGT+CGG | 0.596402 | 8.3:+27144160 | MS.gene89597:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.599548 | 8.3:+27145528 | MS.gene89597:CDS |
| ATGGATTAGAGAAGAAGACT+TGG | 0.600807 | 8.3:+27140896 | MS.gene89597:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.3:+27145540 | MS.gene89597:CDS |
| CTGTTGTCTAGATTATGCAC+AGG | 0.608523 | 8.3:+27141819 | MS.gene89597:CDS |
| TGGCATGAAATCAAAACGCA+AGG | 0.615437 | 8.3:+27146023 | MS.gene89597:CDS |
| GAGGGCATCAAGGATGACTA+CGG | 0.617138 | 8.3:-27140070 | None:intergenic |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.3:+27144255 | MS.gene89597:CDS |
| AGATGATGATGGTACTGAGT+GGG | 0.622629 | 8.3:+27146047 | MS.gene89597:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.625307 | 8.3:+27140902 | MS.gene89597:CDS |
| TGATGGTACTGAGTGGGAAG+AGG | 0.628964 | 8.3:+27146053 | MS.gene89597:CDS |
| GCCATACAGCGGAGATACCA+AGG | 0.630579 | 8.3:+27144311 | MS.gene89597:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.3:+27145534 | MS.gene89597:CDS |
| TTCCATTACAACATTCACAA+TGG | 0.633215 | 8.3:-27142277 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.3:+27145539 | MS.gene89597:CDS |
| GCTACAGCTGATGCTACGAA+AGG | 0.657325 | 8.3:+27141754 | MS.gene89597:CDS |
| GGACATGCTTCAAAAGATGG+AGG | 0.658761 | 8.3:+27142388 | MS.gene89597:CDS |
| TACGAAGGAACAGCGCGGAG+AGG | 0.662953 | 8.3:+27145635 | MS.gene89597:CDS |
| GTATGTGTATTGAGCCGCTG+TGG | 0.682826 | 8.3:-27145694 | None:intergenic |
| ATGAGAATGCAAGAAGACGA+CGG | 0.685899 | 8.3:+27142351 | MS.gene89597:CDS |
| ATCAGGTATGAATCTTACGA+AGG | 0.688860 | 8.3:+27145620 | MS.gene89597:CDS |
| TCTTCAAGAGATATTAACCG+CGG | 0.712756 | 8.3:-27142245 | None:intergenic |
| ACAACGCTTTGGATGCACCG+CGG | 0.721316 | 8.3:+27142228 | MS.gene89597:CDS |
| GGAGTACCACTGCCATACAG+CGG | 0.725601 | 8.3:+27144300 | MS.gene89597:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.3:+27140042 | MS.gene89597:CDS |
| ATGTTGTAATGGAAAACTAG+AGG | 0.731298 | 8.3:+27142286 | MS.gene89597:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.3:-27145603 | None:intergenic |
| AAGATGATGATGGTACTGAG+TGG | 0.777201 | 8.3:+27146046 | MS.gene89597:CDS |
| AATCTTACGAAGGAACAGCG+CGG | 0.828033 | 8.3:+27145630 | MS.gene89597:CDS |
| TGGGACTTCAACTTCCACAG+CGG | 0.838026 | 8.3:+27145680 | MS.gene89597:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 27139804 | 27151672 | 27139804 | ID=MS.gene89597 |
| chr8.3 | mRNA | 27139804 | 27151672 | 27139804 | ID=MS.gene89597.t1;Parent=MS.gene89597 |
| chr8.3 | exon | 27139804 | 27139823 | 27139804 | ID=MS.gene89597.t1.exon1;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27139804 | 27139823 | 27139804 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27139975 | 27140097 | 27139975 | ID=MS.gene89597.t1.exon2;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27139975 | 27140097 | 27139975 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27140890 | 27141010 | 27140890 | ID=MS.gene89597.t1.exon3;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27140890 | 27141010 | 27140890 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27141715 | 27141840 | 27141715 | ID=MS.gene89597.t1.exon4;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27141715 | 27141840 | 27141715 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27142011 | 27142120 | 27142011 | ID=MS.gene89597.t1.exon5;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27142011 | 27142120 | 27142011 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27142226 | 27142409 | 27142226 | ID=MS.gene89597.t1.exon6;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27142226 | 27142409 | 27142226 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27144147 | 27144332 | 27144147 | ID=MS.gene89597.t1.exon7;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27144147 | 27144332 | 27144147 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27145522 | 27145758 | 27145522 | ID=MS.gene89597.t1.exon8;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27145522 | 27145758 | 27145522 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27145895 | 27146087 | 27145895 | ID=MS.gene89597.t1.exon9;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27145895 | 27146087 | 27145895 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| chr8.3 | exon | 27151584 | 27151672 | 27151584 | ID=MS.gene89597.t1.exon10;Parent=MS.gene89597.t1 |
| chr8.3 | CDS | 27151584 | 27151672 | 27151584 | ID=cds.MS.gene89597.t1;Parent=MS.gene89597.t1 |
| Gene Sequence |
| Protein sequence |
