Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90077.t1 | XP_003612893.1 | 97.1 | 137 | 4 | 0 | 1 | 137 | 95 | 231 | 1.40E-65 | 258.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90077.t1 | G7KDT4 | 97.1 | 137 | 4 | 0 | 1 | 137 | 95 | 231 | 1.0e-65 | 258.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene90077 | 0.8176 | 3.03E-52 | -1.69E-46 |
| MS.gene050955 | MS.gene90077 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene051903 | MS.gene90077 | 0.811698 | 6.15E-51 | -1.69E-46 |
| MS.gene052242 | MS.gene90077 | -0.828164 | 1.05E-54 | -1.69E-46 |
| MS.gene054403 | MS.gene90077 | 0.816707 | 4.81E-52 | -1.69E-46 |
| MS.gene054536 | MS.gene90077 | 0.803729 | 3.03E-49 | -1.69E-46 |
| MS.gene057452 | MS.gene90077 | -0.807446 | 5.03E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene90077 | 0.82353 | 1.32E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene90077 | 0.824502 | 7.80E-54 | -1.69E-46 |
| MS.gene058074 | MS.gene90077 | 0.806023 | 1.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90077.t1 | MTR_5g030210 | 97.080 | 137 | 4 | 0 | 1 | 137 | 95 | 231 | 6.93e-92 | 265 |
| MS.gene90077.t1 | MTR_5g430370 | 97.080 | 137 | 4 | 0 | 1 | 137 | 95 | 231 | 6.93e-92 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90077.t1 | AT1G32690 | 44.340 | 106 | 31 | 4 | 33 | 137 | 122 | 200 | 3.42e-14 | 66.6 |
| MS.gene90077.t1 | AT2G35200 | 54.688 | 64 | 21 | 3 | 76 | 137 | 144 | 201 | 2.94e-12 | 61.6 |
Find 50 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTAGAAGCTTTCGATTGC+CGG | 0.319927 | 5.2:+65282 | None:intergenic |
| GAAAGCTTCTAAAGTTGTTC+AGG | 0.349932 | 5.2:-65273 | MS.gene90077:CDS |
| GCAAGTCAGCGAGGTCGTTT+TGG | 0.364935 | 5.2:-65541 | MS.gene90077:CDS |
| AGAGATTCTCCGGCGATTAA+CGG | 0.402315 | 5.2:-65566 | MS.gene90077:CDS |
| GAGTAGAAAAGCGGCGGTTA+TGG | 0.406718 | 5.2:-65417 | MS.gene90077:CDS |
| TTCTTTACCTCCGCCGGCGC+CGG | 0.417595 | 5.2:+65601 | None:intergenic |
| GGAGTGAATCGGAGCGAGAT+TGG | 0.419488 | 5.2:-65481 | MS.gene90077:CDS |
| CGCCGGCGGAGGTAAAGAAT+TGG | 0.424414 | 5.2:-65597 | MS.gene90077:CDS |
| CTCGATCGCGATCGGAGAAA+CGG | 0.428088 | 5.2:+65629 | None:intergenic |
| CGCTTTAAATTCTCCGTCAC+CGG | 0.433054 | 5.2:+65358 | None:intergenic |
| GAACGTTCTCCTTTGTATAG+AGG | 0.436369 | 5.2:-65251 | MS.gene90077:CDS |
| GAGTGAATCGGAGCGAGATT+GGG | 0.455212 | 5.2:-65480 | MS.gene90077:CDS |
| GTCAAGGTCGGTGGCTGTGA+CGG | 0.472779 | 5.2:-65390 | MS.gene90077:CDS |
| CGGAAAACCTTCATCGGACT+CGG | 0.474549 | 5.2:+65302 | None:intergenic |
| GGAGATGAAGAAGGTGTTGA+CGG | 0.481055 | 5.2:-65459 | MS.gene90077:CDS |
| AGATTCTCCGGCGCCGGCGG+AGG | 0.484284 | 5.2:-65608 | MS.gene90077:CDS |
| AAGGTCGGTGGCTGTGACGG+CGG | 0.490130 | 5.2:-65387 | MS.gene90077:CDS |
| ATCGCCGGAGAATCTCTATC+AGG | 0.491493 | 5.2:+65572 | None:intergenic |
| GCTGTGACGGCGGTTTCTGC+CGG | 0.500573 | 5.2:-65377 | MS.gene90077:CDS |
| TTCCAATTCTTTACCTCCGC+CGG | 0.505622 | 5.2:+65595 | None:intergenic |
| CGATCGAGATTCTCCGGCGC+CGG | 0.506756 | 5.2:-65614 | MS.gene90077:CDS |
| CCACCGCGTTCGAAGGGAAA+GGG | 0.516284 | 5.2:-65335 | MS.gene90077:CDS |
| GTTCTTTCCGAGTCCGATGA+AGG | 0.518375 | 5.2:-65309 | MS.gene90077:CDS |
| CATAAGAGTAACAGTAACAG+AGG | 0.524189 | 5.2:-65503 | MS.gene90077:CDS |
| CGATCGCGATCGAGATTCTC+CGG | 0.527728 | 5.2:-65620 | MS.gene90077:CDS |
| TAAAGCGCCACCGCGTTCGA+AGG | 0.528421 | 5.2:-65342 | MS.gene90077:CDS |
| GGAACCTGATAGAGATTCTC+CGG | 0.530069 | 5.2:-65576 | MS.gene90077:CDS |
| GATTGCCGGAAAACCTTCAT+CGG | 0.534304 | 5.2:+65296 | None:intergenic |
| ACGGCGGTTTCTGCCGGTGA+CGG | 0.536770 | 5.2:-65371 | MS.gene90077:CDS |
| CGGAGAATCTCGATCGCGAT+CGG | 0.539017 | 5.2:+65621 | None:intergenic |
| TCGAGATTCTCCGGCGCCGG+CGG | 0.543474 | 5.2:-65611 | MS.gene90077:CDS |
| GCGAGATTGGGAGATGAAGA+AGG | 0.564675 | 5.2:-65468 | MS.gene90077:CDS |
| GACTTGCTTCCGTTAATCGC+CGG | 0.567137 | 5.2:+65557 | None:intergenic |
| CAGTAACAGAGGGAGTGAAT+CGG | 0.569768 | 5.2:-65492 | MS.gene90077:CDS |
| GCCACCGCGTTCGAAGGGAA+AGG | 0.576737 | 5.2:-65336 | MS.gene90077:CDS |
| CCCTTTCCCTTCGAACGCGG+TGG | 0.576863 | 5.2:+65335 | None:intergenic |
| AAAGCGCCACCGCGTTCGAA+GGG | 0.578761 | 5.2:-65341 | MS.gene90077:CDS |
| GAAAAGCGGCGGTTATGGCT+AGG | 0.579162 | 5.2:-65412 | MS.gene90077:CDS |
| CATCCCTTTCCCTTCGAACG+CGG | 0.587084 | 5.2:+65332 | None:intergenic |
| CAATTTCAACCTCTATACAA+AGG | 0.592513 | 5.2:+65242 | None:intergenic |
| GGTTATGGCTAGGTCAAGGT+CGG | 0.593743 | 5.2:-65402 | MS.gene90077:CDS |
| GATGAAGAAGGTGTTGACGG+AGG | 0.604201 | 5.2:-65456 | MS.gene90077:CDS |
| TATGGCTAGGTCAAGGTCGG+TGG | 0.608472 | 5.2:-65399 | MS.gene90077:CDS |
| CGGCGGTTATGGCTAGGTCA+AGG | 0.634980 | 5.2:-65406 | MS.gene90077:CDS |
| CGCGTTCGAAGGGAAAGGGA+TGG | 0.648433 | 5.2:-65331 | MS.gene90077:CDS |
| CGGTGATGTGAGTAGAAAAG+CGG | 0.673609 | 5.2:-65426 | MS.gene90077:CDS |
| TGATGTGAGTAGAAAAGCGG+CGG | 0.691902 | 5.2:-65423 | MS.gene90077:CDS |
| ATAAGAGTAACAGTAACAGA+GGG | 0.693439 | 5.2:-65502 | MS.gene90077:CDS |
| GTGTTGACGGAGGAAATCGA+CGG | 0.751703 | 5.2:-65446 | MS.gene90077:CDS |
| TTAACGGAAGCAAGTCAGCG+AGG | 0.770062 | 5.2:-65550 | MS.gene90077:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGAGTAACAGTAACAGA+GGG | - | chr5.2:65383-65402 | MS.gene90077:CDS | 30.0% | |
| CATAAGAGTAACAGTAACAG+AGG | - | chr5.2:65382-65401 | MS.gene90077:CDS | 35.0% | |
| !! | GAAAGCTTCTAAAGTTGTTC+AGG | - | chr5.2:65612-65631 | MS.gene90077:CDS | 35.0% |
| !! | GTCGTTTTGGTCAATTTTCA+AGG | - | chr5.2:65357-65376 | MS.gene90077:CDS | 35.0% |
| GAACGTTCTCCTTTGTATAG+AGG | - | chr5.2:65634-65653 | MS.gene90077:CDS | 40.0% | |
| ! | CTTTAGAAGCTTTCGATTGC+CGG | + | chr5.2:65606-65625 | None:intergenic | 40.0% |
| AGAGATTCTCCGGCGATTAA+CGG | - | chr5.2:65319-65338 | MS.gene90077:CDS | 45.0% | |
| CAGTAACAGAGGGAGTGAAT+CGG | - | chr5.2:65393-65412 | MS.gene90077:CDS | 45.0% | |
| CGGTGATGTGAGTAGAAAAG+CGG | - | chr5.2:65459-65478 | MS.gene90077:CDS | 45.0% | |
| GATTGCCGGAAAACCTTCAT+CGG | + | chr5.2:65592-65611 | None:intergenic | 45.0% | |
| GGAACCTGATAGAGATTCTC+CGG | - | chr5.2:65309-65328 | MS.gene90077:CDS | 45.0% | |
| TGATGTGAGTAGAAAAGCGG+CGG | - | chr5.2:65462-65481 | MS.gene90077:CDS | 45.0% | |
| TTCCAATTCTTTACCTCCGC+CGG | + | chr5.2:65293-65312 | None:intergenic | 45.0% | |
| ! | CGCTTTAAATTCTCCGTCAC+CGG | + | chr5.2:65530-65549 | None:intergenic | 45.0% |
| !! | GGAGATGAAGAAGGTGTTGA+CGG | - | chr5.2:65426-65445 | MS.gene90077:CDS | 45.0% |
| ATCGCCGGAGAATCTCTATC+AGG | + | chr5.2:65316-65335 | None:intergenic | 50.0% | |
| CGGAAAACCTTCATCGGACT+CGG | + | chr5.2:65586-65605 | None:intergenic | 50.0% | |
| GAGTAGAAAAGCGGCGGTTA+TGG | - | chr5.2:65468-65487 | MS.gene90077:CDS | 50.0% | |
| GAGTGAATCGGAGCGAGATT+GGG | - | chr5.2:65405-65424 | MS.gene90077:CDS | 50.0% | |
| GCGAGATTGGGAGATGAAGA+AGG | - | chr5.2:65417-65436 | MS.gene90077:CDS | 50.0% | |
| GGTTATGGCTAGGTCAAGGT+CGG | - | chr5.2:65483-65502 | MS.gene90077:CDS | 50.0% | |
| GTTCTTTCCGAGTCCGATGA+AGG | - | chr5.2:65576-65595 | MS.gene90077:CDS | 50.0% | |
| TTAACGGAAGCAAGTCAGCG+AGG | - | chr5.2:65335-65354 | MS.gene90077:CDS | 50.0% | |
| ! | CGAGTCCGATGAAGGTTTTC+CGG | - | chr5.2:65584-65603 | MS.gene90077:CDS | 50.0% |
| ! | GACTTGCTTCCGTTAATCGC+CGG | + | chr5.2:65331-65350 | None:intergenic | 50.0% |
| ! | GTGTTGACGGAGGAAATCGA+CGG | - | chr5.2:65439-65458 | MS.gene90077:CDS | 50.0% |
| !! | GATGAAGAAGGTGTTGACGG+AGG | - | chr5.2:65429-65448 | MS.gene90077:CDS | 50.0% |
| CATCCCTTTCCCTTCGAACG+CGG | + | chr5.2:65556-65575 | None:intergenic | 55.0% | |
| CGATCGCGATCGAGATTCTC+CGG | - | chr5.2:65265-65284 | MS.gene90077:CDS | 55.0% | |
| CGGAGAATCTCGATCGCGAT+CGG | + | chr5.2:65267-65286 | None:intergenic | 55.0% | |
| CTCGATCGCGATCGGAGAAA+CGG | + | chr5.2:65259-65278 | None:intergenic | 55.0% | |
| GAAAAGCGGCGGTTATGGCT+AGG | - | chr5.2:65473-65492 | MS.gene90077:CDS | 55.0% | |
| GGAGTGAATCGGAGCGAGAT+TGG | - | chr5.2:65404-65423 | MS.gene90077:CDS | 55.0% | |
| TATGGCTAGGTCAAGGTCGG+TGG | - | chr5.2:65486-65505 | MS.gene90077:CDS | 55.0% | |
| ! | GCAAGTCAGCGAGGTCGTTT+TGG | - | chr5.2:65344-65363 | MS.gene90077:CDS | 55.0% |
| CCACCGCGTTCGAAGGGAAA+GGG | - | chr5.2:65550-65569 | MS.gene90077:CDS | 60.0% | |
| CGCCGGCGGAGGTAAAGAAT+TGG | - | chr5.2:65288-65307 | MS.gene90077:CDS | 60.0% | |
| CGCGTTCGAAGGGAAAGGGA+TGG | - | chr5.2:65554-65573 | MS.gene90077:CDS | 60.0% | |
| CGGCGGTTATGGCTAGGTCA+AGG | - | chr5.2:65479-65498 | MS.gene90077:CDS | 60.0% | |
| GTCAAGGTCGGTGGCTGTGA+CGG | - | chr5.2:65495-65514 | MS.gene90077:CDS | 60.0% | |
| ! | AAAGCGCCACCGCGTTCGAA+GGG | - | chr5.2:65544-65563 | MS.gene90077:CDS | 60.0% |
| ! | TAAAGCGCCACCGCGTTCGA+AGG | - | chr5.2:65543-65562 | MS.gene90077:CDS | 60.0% |
| AAGGTCGGTGGCTGTGACGG+CGG | - | chr5.2:65498-65517 | MS.gene90077:CDS | 65.0% | |
| ACGGCGGTTTCTGCCGGTGA+CGG | - | chr5.2:65514-65533 | MS.gene90077:CDS | 65.0% | |
| CCCTTTCCCTTCGAACGCGG+TGG | + | chr5.2:65553-65572 | None:intergenic | 65.0% | |
| CGATCGAGATTCTCCGGCGC+CGG | - | chr5.2:65271-65290 | MS.gene90077:CDS | 65.0% | |
| GCCACCGCGTTCGAAGGGAA+AGG | - | chr5.2:65549-65568 | MS.gene90077:CDS | 65.0% | |
| GCTGTGACGGCGGTTTCTGC+CGG | - | chr5.2:65508-65527 | MS.gene90077:CDS | 65.0% | |
| TTCTTTACCTCCGCCGGCGC+CGG | + | chr5.2:65287-65306 | None:intergenic | 65.0% | |
| ! | TCGAGATTCTCCGGCGCCGG+CGG | - | chr5.2:65274-65293 | MS.gene90077:CDS | 70.0% |
| !! | AGATTCTCCGGCGCCGGCGG+AGG | - | chr5.2:65277-65296 | MS.gene90077:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 65247 | 65660 | 65247 | ID=MS.gene90077 |
| chr5.2 | mRNA | 65247 | 65660 | 65247 | ID=MS.gene90077.t1;Parent=MS.gene90077 |
| chr5.2 | exon | 65247 | 65660 | 65247 | ID=MS.gene90077.t1.exon1;Parent=MS.gene90077.t1 |
| chr5.2 | CDS | 65247 | 65660 | 65247 | ID=cds.MS.gene90077.t1;Parent=MS.gene90077.t1 |
| Gene Sequence |
| Protein sequence |
