Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90591.t1 | XP_003596535.2 | 97.5 | 161 | 4 | 0 | 1 | 161 | 85 | 245 | 3.10E-85 | 324.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90591.t1 | P21747 | 48.9 | 135 | 67 | 2 | 28 | 161 | 111 | 244 | 5.5e-30 | 132.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90591.t1 | G7ITL0 | 97.5 | 161 | 4 | 0 | 1 | 161 | 85 | 245 | 2.3e-85 | 324.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene90591 | 0.830565 | 2.73E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene90591 | 0.851849 | 6.79E-61 | -1.69E-46 |
| MS.gene051201 | MS.gene90591 | 0.803923 | 2.76E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene90591 | 0.812363 | 4.40E-51 | -1.69E-46 |
| MS.gene051648 | MS.gene90591 | 0.826745 | 2.29E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene90591 | 0.811046 | 8.51E-51 | -1.69E-46 |
| MS.gene052151 | MS.gene90591 | 0.805076 | 1.59E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene90591 | 0.802435 | 5.62E-49 | -1.69E-46 |
| MS.gene052628 | MS.gene90591 | 0.805719 | 1.17E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene90591 | 0.850824 | 1.32E-60 | -1.69E-46 |
| MS.gene053536 | MS.gene90591 | 0.830585 | 2.70E-55 | -1.69E-46 |
| MS.gene053538 | MS.gene90591 | 0.823962 | 1.04E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene90591 | 0.812879 | 3.40E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene90591 | 0.837393 | 5.34E-57 | -1.69E-46 |
| MS.gene056396 | MS.gene90591 | 0.809029 | 2.32E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene90591 | 0.818128 | 2.30E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene90591 | 0.800367 | 1.49E-48 | -1.69E-46 |
| MS.gene059424 | MS.gene90591 | 0.818874 | 1.56E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene90591 | 0.841247 | 5.34E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90591.t1 | MTR_2g081610 | 97.516 | 161 | 4 | 0 | 1 | 161 | 85 | 245 | 3.27e-117 | 332 |
| MS.gene90591.t1 | MTR_2g081590 | 80.503 | 159 | 31 | 0 | 1 | 159 | 85 | 243 | 1.60e-95 | 277 |
| MS.gene90591.t1 | MTR_2g081770 | 69.182 | 159 | 46 | 1 | 1 | 156 | 91 | 249 | 5.45e-76 | 228 |
| MS.gene90591.t1 | MTR_8g445140 | 48.101 | 158 | 74 | 3 | 4 | 161 | 139 | 288 | 8.57e-52 | 167 |
| MS.gene90591.t1 | MTR_8g045890 | 52.593 | 135 | 63 | 1 | 27 | 161 | 284 | 417 | 3.46e-48 | 161 |
| MS.gene90591.t1 | MTR_8g045890 | 52.593 | 135 | 63 | 1 | 27 | 161 | 325 | 458 | 7.84e-48 | 161 |
| MS.gene90591.t1 | MTR_8g045890 | 52.593 | 135 | 63 | 1 | 27 | 161 | 362 | 495 | 1.32e-47 | 161 |
| MS.gene90591.t1 | MTR_8g046000 | 45.860 | 157 | 77 | 2 | 12 | 161 | 53 | 208 | 2.74e-47 | 154 |
| MS.gene90591.t1 | MTR_8g445170 | 44.295 | 149 | 76 | 2 | 13 | 161 | 228 | 369 | 5.06e-44 | 149 |
| MS.gene90591.t1 | MTR_8g044290 | 48.227 | 141 | 66 | 2 | 12 | 152 | 210 | 343 | 3.73e-42 | 145 |
| MS.gene90591.t1 | MTR_8g045880 | 51.852 | 135 | 64 | 1 | 27 | 161 | 167 | 300 | 3.83e-42 | 143 |
| MS.gene90591.t1 | MTR_8g045880 | 51.852 | 135 | 64 | 1 | 27 | 161 | 202 | 335 | 6.64e-42 | 143 |
| MS.gene90591.t1 | MTR_8g045880 | 51.852 | 135 | 64 | 1 | 27 | 161 | 204 | 337 | 6.91e-42 | 143 |
| MS.gene90591.t1 | MTR_1g045860 | 45.161 | 155 | 81 | 3 | 8 | 161 | 94 | 245 | 1.08e-39 | 135 |
| MS.gene90591.t1 | MTR_1g008590 | 45.600 | 125 | 60 | 5 | 44 | 161 | 187 | 310 | 3.87e-28 | 106 |
| MS.gene90591.t1 | MTR_0312s0030 | 45.600 | 125 | 60 | 5 | 44 | 161 | 187 | 310 | 4.25e-28 | 106 |
| MS.gene90591.t1 | MTR_1g007520 | 45.600 | 125 | 60 | 5 | 44 | 161 | 187 | 310 | 4.25e-28 | 106 |
| MS.gene90591.t1 | MTR_3g109490 | 41.085 | 129 | 66 | 5 | 41 | 159 | 177 | 305 | 1.24e-26 | 102 |
| MS.gene90591.t1 | MTR_1g008530 | 39.597 | 149 | 73 | 7 | 28 | 161 | 63 | 209 | 4.48e-26 | 99.0 |
| MS.gene90591.t1 | MTR_1g008470 | 41.270 | 126 | 67 | 5 | 42 | 161 | 161 | 285 | 7.31e-26 | 100 |
| MS.gene90591.t1 | MTR_1g008580 | 39.597 | 149 | 73 | 7 | 28 | 161 | 11 | 157 | 8.03e-26 | 97.1 |
| MS.gene90591.t1 | MTR_1g008560 | 39.597 | 149 | 73 | 7 | 28 | 161 | 169 | 315 | 1.11e-24 | 97.4 |
| MS.gene90591.t1 | MTR_1g008510 | 41.270 | 126 | 65 | 5 | 44 | 161 | 187 | 311 | 1.29e-24 | 97.4 |
| MS.gene90591.t1 | MTR_1g008500 | 40.000 | 125 | 68 | 3 | 44 | 161 | 246 | 370 | 3.87e-24 | 96.7 |
| MS.gene90591.t1 | MTR_1g008460 | 38.211 | 123 | 71 | 3 | 44 | 161 | 160 | 282 | 1.43e-22 | 91.3 |
| MS.gene90591.t1 | MTR_1g008450 | 36.301 | 146 | 80 | 6 | 28 | 161 | 154 | 298 | 4.03e-21 | 87.8 |
| MS.gene90591.t1 | MTR_3g116380 | 33.071 | 127 | 76 | 5 | 44 | 161 | 162 | 288 | 7.49e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90591.t1 | AT5G25610 | 34.756 | 164 | 92 | 6 | 13 | 161 | 214 | 377 | 2.01e-22 | 92.4 |
Find 19 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAATAAAATACAACCTTT+TGG | 0.266567 | 2.3:+16365515 | None:intergenic |
| CGTCCCTTTGCACAACCTTT+TGG | 0.281924 | 2.3:-16365802 | MS.gene90591:CDS |
| AAATCCTTAGGAACATTAAT+CGG | 0.372556 | 2.3:-16365670 | MS.gene90591:CDS |
| GTTTGTCATGCTGATACTTC+AGG | 0.375794 | 2.3:-16365418 | MS.gene90591:CDS |
| GGATTTGCAATTTCTAAGCT+TGG | 0.386794 | 2.3:-16365649 | MS.gene90591:CDS |
| ATAGAGTCAGTGCAAAATCT+TGG | 0.422753 | 2.3:-16365571 | MS.gene90591:CDS |
| AGAAAGCAGTTATGTGTCAT+AGG | 0.437124 | 2.3:-16365546 | MS.gene90591:CDS |
| TTGTTCATGCTTATCTATGA+AGG | 0.440092 | 2.3:+16365600 | None:intergenic |
| ACACCAAAAGGTTGTGCAAA+GGG | 0.481077 | 2.3:+16365799 | None:intergenic |
| ATCCATGTATAAACACCAAA+AGG | 0.486806 | 2.3:+16365787 | None:intergenic |
| GTTCCATCATTAGCCACCAA+TGG | 0.510862 | 2.3:+16365460 | None:intergenic |
| TCATAGGTTGAATTTCCAAA+AGG | 0.512621 | 2.3:-16365530 | MS.gene90591:CDS |
| CAGATTATGAAAGCTGATCC+TGG | 0.522799 | 2.3:-16365373 | MS.gene90591:CDS |
| AAATCCGATTAATGTTCCTA+AGG | 0.524830 | 2.3:+16365666 | None:intergenic |
| GTTCCATTGGTGGCTAATGA+TGG | 0.535070 | 2.3:-16365463 | MS.gene90591:CDS |
| GTAAAGAAAGCAGCTGCAGA+AGG | 0.576977 | 2.3:-16365712 | MS.gene90591:CDS |
| AGAAGGATACACATTTAATG+AGG | 0.587845 | 2.3:-16365740 | MS.gene90591:CDS |
| AACACCAAAAGGTTGTGCAA+AGG | 0.591897 | 2.3:+16365798 | None:intergenic |
| TGACAAACAGGTTTACTACC+AGG | 0.616609 | 2.3:+16365355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAGACATTGAAAAAGA+AGG | - | chr2.3:16365429-16365448 | MS.gene90591:CDS | 20.0% |
| !!! | TTTTATTGTCATGAAATTCA+TGG | - | chr2.3:16365684-16365703 | MS.gene90591:CDS | 20.0% |
| ! | AAATCCTTAGGAACATTAAT+CGG | - | chr2.3:16365516-16365535 | MS.gene90591:CDS | 25.0% |
| !! | AAATTTTGTGCAAAATCCTT+AGG | - | chr2.3:16365504-16365523 | MS.gene90591:CDS | 25.0% |
| !! | GACAATAAAATACAACCTTT+TGG | + | chr2.3:16365674-16365693 | None:intergenic | 25.0% |
| AAATCCGATTAATGTTCCTA+AGG | + | chr2.3:16365523-16365542 | None:intergenic | 30.0% | |
| AGAAGGATACACATTTAATG+AGG | - | chr2.3:16365446-16365465 | MS.gene90591:CDS | 30.0% | |
| ATCCATGTATAAACACCAAA+AGG | + | chr2.3:16365402-16365421 | None:intergenic | 30.0% | |
| TTGTTCATGCTTATCTATGA+AGG | + | chr2.3:16365589-16365608 | None:intergenic | 30.0% | |
| ! | TCATAGGTTGAATTTCCAAA+AGG | - | chr2.3:16365656-16365675 | MS.gene90591:CDS | 30.0% |
| !!! | AACCTTTTGGTGTTTATACA+TGG | - | chr2.3:16365397-16365416 | MS.gene90591:CDS | 30.0% |
| AGAAAGCAGTTATGTGTCAT+AGG | - | chr2.3:16365640-16365659 | MS.gene90591:CDS | 35.0% | |
| ATAGAGTCAGTGCAAAATCT+TGG | - | chr2.3:16365615-16365634 | MS.gene90591:CDS | 35.0% | |
| GGATTTGCAATTTCTAAGCT+TGG | - | chr2.3:16365537-16365556 | MS.gene90591:CDS | 35.0% | |
| TTGCTGAATTGCACTTTCAT+AGG | + | chr2.3:16365363-16365382 | None:intergenic | 35.0% | |
| ! | AACAGCTTTTATGGTTCCAT+TGG | - | chr2.3:16365710-16365729 | MS.gene90591:CDS | 35.0% |
| ! | TCATGGAACAACAGCTTTTA+TGG | - | chr2.3:16365701-16365720 | MS.gene90591:CDS | 35.0% |
| AACACCAAAAGGTTGTGCAA+AGG | + | chr2.3:16365391-16365410 | None:intergenic | 40.0% | |
| ACACCAAAAGGTTGTGCAAA+GGG | + | chr2.3:16365390-16365409 | None:intergenic | 40.0% | |
| CAGATTATGAAAGCTGATCC+TGG | - | chr2.3:16365813-16365832 | MS.gene90591:CDS | 40.0% | |
| GTTTGTCATGCTGATACTTC+AGG | - | chr2.3:16365768-16365787 | MS.gene90591:CDS | 40.0% | |
| ! | AGCTTTTATGGTTCCATTGG+TGG | - | chr2.3:16365713-16365732 | MS.gene90591:CDS | 40.0% |
| GTAAAGAAAGCAGCTGCAGA+AGG | - | chr2.3:16365474-16365493 | MS.gene90591:CDS | 45.0% | |
| GTTCCATCATTAGCCACCAA+TGG | + | chr2.3:16365729-16365748 | None:intergenic | 45.0% | |
| ! | GTTCCATTGGTGGCTAATGA+TGG | - | chr2.3:16365723-16365742 | MS.gene90591:CDS | 45.0% |
| ! | CGTCCCTTTGCACAACCTTT+TGG | - | chr2.3:16365384-16365403 | MS.gene90591:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 16365363 | 16365845 | 16365363 | ID=MS.gene90591 |
| chr2.3 | mRNA | 16365363 | 16365845 | 16365363 | ID=MS.gene90591.t1;Parent=MS.gene90591 |
| chr2.3 | exon | 16365363 | 16365845 | 16365363 | ID=MS.gene90591.t1.exon1;Parent=MS.gene90591.t1 |
| chr2.3 | CDS | 16365363 | 16365845 | 16365363 | ID=cds.MS.gene90591.t1;Parent=MS.gene90591.t1 |
| Gene Sequence |
| Protein sequence |
