AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90980.t1 MTR_4g073800 88.189 254 10 1 1 234 1 254 5.61e-156 434
MS.gene90980.t1 MTR_5g009720 70.136 221 64 1 1 221 1 219 2.79e-109 314
MS.gene90980.t1 MTR_8g099190 65.801 231 67 2 1 221 1 229 1.24e-104 303
MS.gene90980.t1 MTR_8g099190 62.551 243 67 3 1 221 1 241 7.77e-101 294
MS.gene90980.t1 MTR_8g099190 60.086 233 62 4 1 221 1 214 2.91e-90 266
MS.gene90980.t1 MTR_8g099190 62.121 198 63 2 1 188 1 196 1.19e-81 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90980.t1 AT3G08560 68.444 225 71 0 1 225 1 225 5.74e-109 314
MS.gene90980.t1 AT4G11150 70.000 220 64 1 1 220 1 218 9.07e-108 310
MS.gene90980.t1 AT1G64200 63.136 236 79 2 1 230 1 234 1.84e-100 292
MS.gene90980.t1 AT1G64200 64.758 227 72 2 1 221 1 225 1.23e-99 290
MS.gene90980.t1 AT1G64200 63.441 186 62 1 1 180 1 186 6.94e-79 236

Find 40 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTTCTCCTCAGCTTCT+TGG 0.251565 4.2:-34818061 None:intergenic
CGATCATCAAGGGCAATCTT+AGG 0.255958 4.2:-34820367 None:intergenic
TATGAAAGATGATGCTAAAA+AGG 0.304718 4.2:+34818712 MS.gene90980:CDS
GCTGAATACATCTCGTATAA+AGG 0.375872 4.2:+34818658 MS.gene90980:CDS
AATAGAAGGATATACCAAAA+AGG 0.385736 4.2:-34820439 None:intergenic
CATCAAAGAAATAATTGTTC+AGG 0.393403 4.2:+34818778 MS.gene90980:CDS
TCGACGAACATCAATCTGTT+TGG 0.411208 4.2:-34818455 None:intergenic
GATCATCAAGGGCAATCTTA+GGG 0.420043 4.2:-34820366 None:intergenic
AGCAAATCCAGCAGATGATT+AGG 0.425110 4.2:+34818030 MS.gene90980:CDS
TTCTATATTGCCACATCTTG+TGG 0.427568 4.2:+34821523 MS.gene90980:CDS
CTTTCTCCTCAGCTTCTTGG+CGG 0.494892 4.2:-34818058 None:intergenic
TGAAATATCTGTTGCTGCAG+AGG 0.507699 4.2:+34818085 MS.gene90980:CDS
TCACCACCTCATCCTGTGCT+TGG 0.514232 4.2:-34818685 None:intergenic
GGATGAACCTAATCATCTGC+TGG 0.525749 4.2:-34818037 None:intergenic
GTAGTATTGGCCTCAGAGGA+TGG 0.536883 4.2:+34821267 MS.gene90980:CDS
GGTACTCCAAGCACAGGATG+AGG 0.559843 4.2:+34818679 MS.gene90980:CDS
GTAAGGTAATGAACATGCCT+CGG 0.560359 4.2:-34821352 None:intergenic
GGAGATAAACACGATCATCA+AGG 0.564384 4.2:-34820378 None:intergenic
TAGTTCTGGAGGAGTAGTAT+TGG 0.579965 4.2:+34821254 MS.gene90980:intron
TTATTTCAATGTAGTTCTGG+AGG 0.583060 4.2:+34821243 MS.gene90980:intron
GCTTGTTGAGTCGTTGCTAG+AGG 0.584576 4.2:+34820303 MS.gene90980:CDS
TAAGGTAATGAACATGCCTC+GGG 0.588686 4.2:-34821351 None:intergenic
CAGACAAGAGTACGAGCGAA+AGG 0.590203 4.2:+34818432 MS.gene90980:CDS
TATAAAGGTACTCCAAGCAC+AGG 0.593557 4.2:+34818673 MS.gene90980:CDS
CTACATCGTAGTAGCACAGA+TGG 0.593942 4.2:-34820265 None:intergenic
AGAGGATGGAAAGATAGTGT+TGG 0.594931 4.2:+34821281 MS.gene90980:CDS
TGATCAAGTTCCACAAGATG+TGG 0.600195 4.2:-34821533 None:intergenic
GTAGCACAGATGGTTCCCTC+AGG 0.603951 4.2:-34820255 None:intergenic
TGTAGGGTTTACTACGCCTG+AGG 0.609492 4.2:+34820239 MS.gene90980:intron
GAGATAAACACGATCATCAA+GGG 0.613062 4.2:-34820377 None:intergenic
ATCATCAAGGGCAATCTTAG+GGG 0.623318 4.2:-34820365 None:intergenic
GTTCATCCGCCAAGAAGCTG+AGG 0.630671 4.2:+34818052 MS.gene90980:CDS
TCATCAAGGGCAATCTTAGG+GGG 0.635912 4.2:-34820364 None:intergenic
TGTGCTACTACGATGTAGAG+AGG 0.638418 4.2:+34820270 MS.gene90980:CDS
AGGAGTAGTATTGGCCTCAG+AGG 0.646156 4.2:+34821263 MS.gene90980:CDS
GTGCTACTACGATGTAGAGA+GGG 0.653633 4.2:+34820271 MS.gene90980:CDS
CACTATCTTTCCATCCTCTG+AGG 0.654145 4.2:-34821277 None:intergenic
GTAGGGTTTACTACGCCTGA+GGG 0.660356 4.2:+34820240 MS.gene90980:intron
TAGCTTAAAGTTGAACAAGA+CGG 0.663482 4.2:+34821489 MS.gene90980:CDS
ACTCCAAGCACAGGATGAGG+TGG 0.665263 4.2:+34818682 MS.gene90980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAACAATTATACATA+TGG - chr4.2:34819484-34819503 None:intergenic 10.0%
!! ATTTATTTCAATTCAAAATT+AGG + chr4.2:34818583-34818602 MS.gene90980:intron 10.0%
!! TTAATGAAAATGATAAAATT+TGG - chr4.2:34818845-34818864 None:intergenic 10.0%
!! TTTAATAATATGAATATCAA+TGG + chr4.2:34818308-34818327 MS.gene90980:intron 10.0%
!!! ATTTTTTTTTATTGAAAACA+AGG - chr4.2:34821019-34821038 None:intergenic 10.0%
!!! TGTTTTCAATAAAAAAAAAT+GGG + chr4.2:34821019-34821038 MS.gene90980:intron 10.0%
!!! TTATAAATAGAAATATTTTC+GGG + chr4.2:34819537-34819556 MS.gene90980:intron 10.0%
!!! TTGTTTTCAATAAAAAAAAA+TGG + chr4.2:34821018-34821037 MS.gene90980:intron 10.0%
!! AAATAATAATTAGACAAGTT+AGG + chr4.2:34818140-34818159 MS.gene90980:intron 15.0%
!! ACGTTTATTTATATAGTTTA+AGG + chr4.2:34820848-34820867 MS.gene90980:intron 15.0%
!! CATAATATAAAATGTCATAA+TGG - chr4.2:34820909-34820928 None:intergenic 15.0%
!! TTTAATCAAAGATAAATAGA+AGG - chr4.2:34820456-34820475 None:intergenic 15.0%
!!! ATTTCTCCTAATTTTTTATA+GGG + chr4.2:34819004-34819023 MS.gene90980:intron 15.0%
!!! TTATGAAAACTTACTTATTT+TGG + chr4.2:34819141-34819160 MS.gene90980:intron 15.0%
!! AAAGTTATGTAATCATTACT+TGG - chr4.2:34819120-34819139 None:intergenic 20.0%
!! TAAAAAATTAGGAGAAATGA+AGG - chr4.2:34819002-34819021 None:intergenic 20.0%
!! TAATATGAATATCAATGGAT+TGG + chr4.2:34818313-34818332 MS.gene90980:intron 20.0%
!! TAGAATTTGGGAATTAAATA+TGG - chr4.2:34819929-34819948 None:intergenic 20.0%
!! TATCATTATGTTCAATTTAG+TGG + chr4.2:34820635-34820654 MS.gene90980:intron 20.0%
!! TATTTATTTCAATGTAGTTC+TGG + chr4.2:34821240-34821259 MS.gene90980:intron 20.0%
!!! AAACAATTAGAAAGTACAAA+GGG - chr4.2:34820489-34820508 None:intergenic 20.0%
!!! AATAAACAAAGTTTTGTCAA+AGG - chr4.2:34818612-34818631 None:intergenic 20.0%
!!! AGTAAACTTTTACAACAAAT+TGG - chr4.2:34820986-34821005 None:intergenic 20.0%
!!! ATATAAAATGTCATAATGGA+CGG - chr4.2:34820905-34820924 None:intergenic 20.0%
!!! ATTATGGTATAACATTTTAG+TGG - chr4.2:34819860-34819879 None:intergenic 20.0%
!!! CAGTTTTTAATACTTAGTTA+GGG + chr4.2:34818925-34818944 MS.gene90980:intron 20.0%
!!! CATTTCTCCTAATTTTTTAT+AGG + chr4.2:34819003-34819022 MS.gene90980:intron 20.0%
!!! TGTGTTTTTTATATTGTTGT+TGG - chr4.2:34818878-34818897 None:intergenic 20.0%
!!! TTAGCATTCACTTTTTTATA+AGG + chr4.2:34819196-34819215 MS.gene90980:intron 20.0%
! AAATCAATGTAATGAGTGTA+AGG - chr4.2:34821372-34821391 None:intergenic 25.0%
! AAATTAAGTGTACAAGTCTA+AGG - chr4.2:34820024-34820043 None:intergenic 25.0%
! AATAGAAGGATATACCAAAA+AGG - chr4.2:34820442-34820461 None:intergenic 25.0%
! AATTAAGTGTACAAGTCTAA+GGG - chr4.2:34820023-34820042 None:intergenic 25.0%
! AATTACTTGAAGCTGAAAAA+AGG + chr4.2:34818404-34818423 MS.gene90980:CDS 25.0%
! AATTGTTCGATTAAGATGTA+TGG - chr4.2:34820590-34820609 None:intergenic 25.0%
! AATTTATTGCATGTGTTTGT+AGG + chr4.2:34820222-34820241 MS.gene90980:intron 25.0%
! ATTTATTGCATGTGTTTGTA+GGG + chr4.2:34820223-34820242 MS.gene90980:intron 25.0%
! CATCAAAGAAATAATTGTTC+AGG + chr4.2:34818778-34818797 MS.gene90980:CDS 25.0%
! GATAAGAATAAAGAGATAGA+GGG - chr4.2:34820079-34820098 None:intergenic 25.0%
! TACCTTAATTGTTAGTGAAA+AGG + chr4.2:34821411-34821430 MS.gene90980:intron 25.0%
! TATCAATTAAAAATCAACGC+TGG - chr4.2:34818246-34818265 None:intergenic 25.0%
! TATCTGATTGGTATATGTAA+AGG - chr4.2:34819772-34819791 None:intergenic 25.0%
! TATGAAAGATGATGCTAAAA+AGG + chr4.2:34818712-34818731 MS.gene90980:CDS 25.0%
! TCTCTACTATTAAGAAGTAA+GGG + chr4.2:34820716-34820735 MS.gene90980:intron 25.0%
! TGATAAGAATAAAGAGATAG+AGG - chr4.2:34820080-34820099 None:intergenic 25.0%
! TTAATTGGATACTGTTTGTT+TGG - chr4.2:34819980-34819999 None:intergenic 25.0%
!! AAAAGCAATAGAAAGTACAA+AGG - chr4.2:34820547-34820566 None:intergenic 25.0%
!! AAAGCAATAGAAAGTACAAA+GGG - chr4.2:34820546-34820565 None:intergenic 25.0%
!! AACCTTTTCACTAACAATTA+AGG - chr4.2:34821416-34821435 None:intergenic 25.0%
!! AGTTAGGGTTAATTAATTTG+TGG + chr4.2:34818940-34818959 MS.gene90980:intron 25.0%
!! ATATACTTTACGGACTAAAT+TGG + chr4.2:34820764-34820783 MS.gene90980:intron 25.0%
!! CAAACAATTAGAAAGTACAA+AGG - chr4.2:34820490-34820509 None:intergenic 25.0%
!! CAATTGTTGTGATTCATTTT+AGG + chr4.2:34818360-34818379 MS.gene90980:intron 25.0%
!! GAATTAAATATGGTGATGTT+GGG - chr4.2:34819919-34819938 None:intergenic 25.0%
!! GCAGTTTTTAATACTTAGTT+AGG + chr4.2:34818924-34818943 MS.gene90980:intron 25.0%
!!! ATATTTTCTAGTAGCATTGA+AGG - chr4.2:34821130-34821149 None:intergenic 25.0%
!!! ATTCAAATTCTGGTTCTTAT+GGG + chr4.2:34818193-34818212 MS.gene90980:intron 25.0%
!!! GAAATTGATGGATTTTGTAT+AGG - chr4.2:34820694-34820713 None:intergenic 25.0%
!!! GGTTTTTGAAAGGTATTTAT+GGG + chr4.2:34819334-34819353 MS.gene90980:intron 25.0%
!!! TGTTCAACTCAGTTTTAAAT+TGG - chr4.2:34819385-34819404 None:intergenic 25.0%
ACAAATTGGCATGATAAACA+AGG - chr4.2:34820972-34820991 None:intergenic 30.0%
AGGATCTATGTATACTACAT+CGG - chr4.2:34819287-34819306 None:intergenic 30.0%
ATAAAGAGATAGAGGGAAAA+TGG - chr4.2:34820072-34820091 None:intergenic 30.0%
ATAGTAGAGACTGAAATTGA+TGG - chr4.2:34820706-34820725 None:intergenic 30.0%
ATGTCGATCAAAAGAGATTA+TGG - chr4.2:34819876-34819895 None:intergenic 30.0%
CAGCTAGCATTATTAACAAT+TGG - chr4.2:34820192-34820211 None:intergenic 30.0%
GCAAGACCCTATAAAAAATT+AGG - chr4.2:34819013-34819032 None:intergenic 30.0%
GGTGAAATTTACATAGCTTA+GGG + chr4.2:34820785-34820804 MS.gene90980:intron 30.0%
GTCTCTACTATTAAGAAGTA+AGG + chr4.2:34820715-34820734 MS.gene90980:intron 30.0%
GTGAAATTTACATAGCTTAG+GGG + chr4.2:34820786-34820805 MS.gene90980:intron 30.0%
TAATTAGACAAGTTAGGTCT+TGG + chr4.2:34818146-34818165 MS.gene90980:intron 30.0%
TAGCTTAAAGTTGAACAAGA+CGG + chr4.2:34821489-34821508 MS.gene90980:CDS 30.0%
TGGTGAAATTTACATAGCTT+AGG + chr4.2:34820784-34820803 MS.gene90980:intron 30.0%
! AGGGAAAATGGAGTATTAAA+AGG - chr4.2:34820060-34820079 None:intergenic 30.0%
! CACATGGAATCAACATTTTT+GGG - chr4.2:34819564-34819583 None:intergenic 30.0%
! GGAATTAAATATGGTGATGT+TGG - chr4.2:34819920-34819939 None:intergenic 30.0%
! TATATGTAAAGGTGTAAGGT+TGG - chr4.2:34819761-34819780 None:intergenic 30.0%
! TGAGTTGAATCCTTATCAAT+CGG + chr4.2:34818219-34818238 MS.gene90980:intron 30.0%
! TTGGTATATGTAAAGGTGTA+AGG - chr4.2:34819765-34819784 None:intergenic 30.0%
!! GAAAGGTATTTATGGGTAAT+GGG + chr4.2:34819341-34819360 MS.gene90980:intron 30.0%
!! GATGGATTTTGTATAGGTTA+AGG - chr4.2:34820688-34820707 None:intergenic 30.0%
!! GATTCAAATTCTGGTTCTTA+TGG + chr4.2:34818192-34818211 MS.gene90980:intron 30.0%
!! TGAAAGGTATTTATGGGTAA+TGG + chr4.2:34819340-34819359 MS.gene90980:intron 30.0%
!! TTATTTCAATGTAGTTCTGG+AGG + chr4.2:34821243-34821262 MS.gene90980:intron 30.0%
!!! GGGTTTTTGAAAGGTATTTA+TGG + chr4.2:34819333-34819352 MS.gene90980:intron 30.0%
AAAGGAAGGTGATCGTAATA+GGG - chr4.2:34819827-34819846 None:intergenic 35.0%
AACGAATTACAATGCTCTAG+TGG - chr4.2:34819438-34819457 None:intergenic 35.0%
AATGCTAACCAATGTCCAAA+GGG - chr4.2:34819184-34819203 None:intergenic 35.0%
ATCTATGTATACTACATCGG+AGG - chr4.2:34819284-34819303 None:intergenic 35.0%
CAAAAACCAATCTACCACTA+GGG - chr4.2:34820151-34820170 None:intergenic 35.0%
CTTTCAAAAACCCCACTATT+AGG - chr4.2:34819326-34819345 None:intergenic 35.0%
GAGATAAACACGATCATCAA+GGG - chr4.2:34820380-34820399 None:intergenic 35.0%
GCTGAATACATCTCGTATAA+AGG + chr4.2:34818658-34818677 MS.gene90980:CDS 35.0%
GGATACTGTTTGTTTGGATA+AGG - chr4.2:34819974-34819993 None:intergenic 35.0%
GTTTGTTTGGATAAGGAGAA+TGG - chr4.2:34819967-34819986 None:intergenic 35.0%
TAATAATGCTAGCTGAGACA+GGG + chr4.2:34820197-34820216 MS.gene90980:intron 35.0%
TACATAGATCCTTGCATTCT+TGG + chr4.2:34819295-34819314 MS.gene90980:intron 35.0%
TCAACCAAAAAATGACGATG+TGG + chr4.2:34820405-34820424 MS.gene90980:CDS 35.0%
TCTTGGAATGTCCTAATAGT+GGG + chr4.2:34819312-34819331 MS.gene90980:intron 35.0%
TGTATAGGTTAAGGACTACT+TGG - chr4.2:34820679-34820698 None:intergenic 35.0%
TTAATAATGCTAGCTGAGAC+AGG + chr4.2:34820196-34820215 MS.gene90980:intron 35.0%
TTCTATATTGCCACATCTTG+TGG + chr4.2:34821523-34821542 MS.gene90980:CDS 35.0%
TTCTTGGAATGTCCTAATAG+TGG + chr4.2:34819311-34819330 MS.gene90980:intron 35.0%
! ACACAATTTCGTGGGTTAAT+TGG - chr4.2:34819995-34820014 None:intergenic 35.0%
! ATATGGTGATGTTGGGTAAA+TGG - chr4.2:34819912-34819931 None:intergenic 35.0%
! GCACATGGAATCAACATTTT+TGG - chr4.2:34819565-34819584 None:intergenic 35.0%
! TAGAAAGTACAAAGGGTTCA+TGG - chr4.2:34820482-34820501 None:intergenic 35.0%
!! AACATTTTAGTGGCAAGGTA+TGG - chr4.2:34819850-34819869 None:intergenic 35.0%
!! ACTTGGTAGAAGTATCTGAT+TGG - chr4.2:34819784-34819803 None:intergenic 35.0%
!! CAGTTGGCTTGAAAACAATT+TGG - chr4.2:34819731-34819750 None:intergenic 35.0%
!! CTAATAGTGGGGTTTTTGAA+AGG + chr4.2:34819324-34819343 MS.gene90980:intron 35.0%
!! GGTATAACATTTTAGTGGCA+AGG - chr4.2:34819855-34819874 None:intergenic 35.0%
!!! ACATCGTCATTTTTTGGTTG+AGG - chr4.2:34820406-34820425 None:intergenic 35.0%
!!! CTAGTGGTAGATTGGTTTTT+GGG + chr4.2:34820150-34820169 MS.gene90980:intron 35.0%
!!! GTATCCACATCGTCATTTTT+TGG - chr4.2:34820412-34820431 None:intergenic 35.0%
AATTTACATAGCTTAGGGGC+TGG + chr4.2:34820790-34820809 MS.gene90980:intron 40.0%
AGAGGATGGAAAGATAGTGT+TGG + chr4.2:34821281-34821300 MS.gene90980:CDS 40.0%
AGCAAATCCAGCAGATGATT+AGG + chr4.2:34818030-34818049 MS.gene90980:CDS 40.0%
ATATGCCCTGAGGATACTAA+TGG - chr4.2:34819039-34819058 None:intergenic 40.0%
ATCATCAAGGGCAATCTTAG+GGG - chr4.2:34820368-34820387 None:intergenic 40.0%
CCAAAAACCAATCTACCACT+AGG - chr4.2:34820152-34820171 None:intergenic 40.0%
CCACTAGGGAGAAAAAGAAA+AGG - chr4.2:34820137-34820156 None:intergenic 40.0%
CGGCAACAAGATTCAAATTC+TGG + chr4.2:34818183-34818202 MS.gene90980:intron 40.0%
CTTGGAATGTCCTAATAGTG+GGG + chr4.2:34819313-34819332 MS.gene90980:intron 40.0%
GAAAGGAAGGTGATCGTAAT+AGG - chr4.2:34819828-34819847 None:intergenic 40.0%
GAATGCTAACCAATGTCCAA+AGG - chr4.2:34819185-34819204 None:intergenic 40.0%
GATCATCAAGGGCAATCTTA+GGG - chr4.2:34820369-34820388 None:intergenic 40.0%
GCTAACCATTAGTATCCTCA+GGG + chr4.2:34819031-34819050 MS.gene90980:intron 40.0%
GCTTGAAAACAATTTGGTCC+GGG - chr4.2:34819725-34819744 None:intergenic 40.0%
GGAGATAAACACGATCATCA+AGG - chr4.2:34820381-34820400 None:intergenic 40.0%
GGCTTGAAAACAATTTGGTC+CGG - chr4.2:34819726-34819745 None:intergenic 40.0%
GTAAGGTAATGAACATGCCT+CGG - chr4.2:34821355-34821374 None:intergenic 40.0%
GTCGAGGGTTCATAGAATTT+GGG - chr4.2:34819941-34819960 None:intergenic 40.0%
GTTTAACGTCCAATACCCTT+TGG + chr4.2:34819166-34819185 MS.gene90980:intron 40.0%
GTTTGGATAAGGAGAATGGA+GGG - chr4.2:34819963-34819982 None:intergenic 40.0%
TAAGGTAATGAACATGCCTC+GGG - chr4.2:34821354-34821373 None:intergenic 40.0%
TAGGACATTCCAAGAATGCA+AGG - chr4.2:34819307-34819326 None:intergenic 40.0%
TCGACGAACATCAATCTGTT+TGG - chr4.2:34818458-34818477 None:intergenic 40.0%
TGAAATATCTGTTGCTGCAG+AGG + chr4.2:34818085-34818104 MS.gene90980:CDS 40.0%
TGATGTTGGGTAAATGGTGT+TGG - chr4.2:34819906-34819925 None:intergenic 40.0%
TGCTAACCATTAGTATCCTC+AGG + chr4.2:34819030-34819049 MS.gene90980:intron 40.0%
TGTTTGGATAAGGAGAATGG+AGG - chr4.2:34819964-34819983 None:intergenic 40.0%
TTAGTATCCTCAGGGCATAT+TGG + chr4.2:34819039-34819058 MS.gene90980:intron 40.0%
TTTAGTGGCAAGGTATGGAA+AGG - chr4.2:34819845-34819864 None:intergenic 40.0%
! AAAGGTGTAAGGTTGGCATT+GGG - chr4.2:34819754-34819773 None:intergenic 40.0%
! AACCAATGTCCAAAGGGTAT+TGG - chr4.2:34819178-34819197 None:intergenic 40.0%
! ATGGACTGTTAGGATCAACT+TGG - chr4.2:34819801-34819820 None:intergenic 40.0%
! CCTTTTCTTTTTCTCCCTAG+TGG + chr4.2:34820134-34820153 MS.gene90980:intron 40.0%
! TAAAGGTGTAAGGTTGGCAT+TGG - chr4.2:34819755-34819774 None:intergenic 40.0%
! TGGATACGCATGAACCTTTT+TGG + chr4.2:34820425-34820444 MS.gene90980:CDS 40.0%
! TTTTCTCCCTAGTGGTAGAT+TGG + chr4.2:34820142-34820161 MS.gene90980:intron 40.0%
!! AATAGGGAAGATGGACTGTT+AGG - chr4.2:34819811-34819830 None:intergenic 40.0%
!! CAATTATGCGAAGCTTTGAG+AGG - chr4.2:34819635-34819654 None:intergenic 40.0%
!! TAGTTCTGGAGGAGTAGTAT+TGG + chr4.2:34821254-34821273 MS.gene90980:intron 40.0%
!! TATAAAGGTACTCCAAGCAC+AGG + chr4.2:34818673-34818692 MS.gene90980:CDS 40.0%
!!! CCTAGTGGTAGATTGGTTTT+TGG + chr4.2:34820149-34820168 MS.gene90980:intron 40.0%
!!! CGTCATTTTTTGGTTGAGGT+GGG - chr4.2:34820402-34820421 None:intergenic 40.0%
!!! TCGTCATTTTTTGGTTGAGG+TGG - chr4.2:34820403-34820422 None:intergenic 40.0%
ATGAAGGTGACGATCAGACA+AGG - chr4.2:34818986-34819005 None:intergenic 45.0%
CACTATCTTTCCATCCTCTG+AGG - chr4.2:34821280-34821299 None:intergenic 45.0%
CGATCATCAAGGGCAATCTT+AGG - chr4.2:34820370-34820389 None:intergenic 45.0%
CTACATCGTAGTAGCACAGA+TGG - chr4.2:34820268-34820287 None:intergenic 45.0%
GCATCAACCCATAACCATTG+AGG - chr4.2:34819598-34819617 None:intergenic 45.0%
GGATGAACCTAATCATCTGC+TGG - chr4.2:34818040-34818059 None:intergenic 45.0%
GGGCACTTACACAATTTCGT+GGG - chr4.2:34820003-34820022 None:intergenic 45.0%
GGTCGAGGGTTCATAGAATT+TGG - chr4.2:34819942-34819961 None:intergenic 45.0%
GGTGATCGTAATAGGGAAGA+TGG - chr4.2:34819820-34819839 None:intergenic 45.0%
GTCCAATACCCTTTGGACAT+TGG + chr4.2:34819173-34819192 MS.gene90980:intron 45.0%
TAGCTTTCTCCTCAGCTTCT+TGG - chr4.2:34818064-34818083 None:intergenic 45.0%
TCATCAAGGGCAATCTTAGG+GGG - chr4.2:34820367-34820386 None:intergenic 45.0%
TCCTTAACCAATATGCCCTG+AGG - chr4.2:34819049-34819068 None:intergenic 45.0%
TCTTGGTCAGAGACATGCAT+CGG + chr4.2:34818163-34818182 MS.gene90980:intron 45.0%
TGAAAACAATTTGGTCCGGG+TGG - chr4.2:34819722-34819741 None:intergenic 45.0%
TGGCAAGACAGACTACACAT+GGG - chr4.2:34819418-34819437 None:intergenic 45.0%
! AGGGCACTTACACAATTTCG+TGG - chr4.2:34820004-34820023 None:intergenic 45.0%
! GTGCTACTACGATGTAGAGA+GGG + chr4.2:34820271-34820290 MS.gene90980:CDS 45.0%
! TCCTCAGGGCATATTGGTTA+AGG + chr4.2:34819045-34819064 MS.gene90980:intron 45.0%
! TGTGCTACTACGATGTAGAG+AGG + chr4.2:34820270-34820289 MS.gene90980:CDS 45.0%
! TGTTGGGTAAATGGTGTTGG+AGG - chr4.2:34819903-34819922 None:intergenic 45.0%
! TTTTCGACAAAAGTTGCCCG+AGG + chr4.2:34821335-34821354 MS.gene90980:CDS 45.0%
!!! ATATTTCTATTTATAATATA+AGG - chr4.2:34819533-34819552 None:intergenic 5.0%
!!! ATTATAAATAGAAATATTTT+CGG + chr4.2:34819536-34819555 MS.gene90980:intron 5.0%
!!! TACAAATAAAAATTTTATTT+TGG + chr4.2:34819072-34819091 MS.gene90980:intron 5.0%
!!! TTATATAGTTTTTTTTTTTT+TGG + chr4.2:34820822-34820841 MS.gene90980:intron 5.0%
!!! TTTAATTTTAATATACTTTA+CGG + chr4.2:34820754-34820773 MS.gene90980:intron 5.0%
AACAATTTGGTCCGGGTGGT+TGG - chr4.2:34819718-34819737 None:intergenic 50.0%
AACCCATAACCATTGAGGCC+GGG - chr4.2:34819593-34819612 None:intergenic 50.0%
ATAAGGAGAATGGAGGGTCG+AGG - chr4.2:34819957-34819976 None:intergenic 50.0%
CAACCCATAACCATTGAGGC+CGG - chr4.2:34819594-34819613 None:intergenic 50.0%
CAACGCTGGTCCGATTGATA+AGG - chr4.2:34818232-34818251 None:intergenic 50.0%
CTTTCTCCTCAGCTTCTTGG+CGG - chr4.2:34818061-34818080 None:intergenic 50.0%
GTAGGGTTTACTACGCCTGA+GGG + chr4.2:34820240-34820259 MS.gene90980:intron 50.0%
GTGGCAAGACAGACTACACA+TGG - chr4.2:34819419-34819438 None:intergenic 50.0%
GTGGCAAGGTATGGAAAGGA+AGG - chr4.2:34819841-34819860 None:intergenic 50.0%
TAAGGAGAATGGAGGGTCGA+GGG - chr4.2:34819956-34819975 None:intergenic 50.0%
TGATTCCATGTGCCATAGCC+CGG + chr4.2:34819572-34819591 MS.gene90980:intron 50.0%
TGTAGGGTTTACTACGCCTG+AGG + chr4.2:34820239-34820258 MS.gene90980:intron 50.0%
! CAGACAAGAGTACGAGCGAA+AGG + chr4.2:34818432-34818451 MS.gene90980:CDS 50.0%
! GCTTGTTGAGTCGTTGCTAG+AGG + chr4.2:34820303-34820322 MS.gene90980:CDS 50.0%
! TAAGGTTGGCATTGGGCAGT+TGG - chr4.2:34819747-34819766 None:intergenic 50.0%
! TACATAGCTTAGGGGCTGGT+TGG + chr4.2:34820794-34820813 MS.gene90980:intron 50.0%
!! AGGAGTAGTATTGGCCTCAG+AGG + chr4.2:34821263-34821282 MS.gene90980:CDS 50.0%
!! GTAGTATTGGCCTCAGAGGA+TGG + chr4.2:34821267-34821286 MS.gene90980:CDS 50.0%
ACTCCAAGCACAGGATGAGG+TGG + chr4.2:34818682-34818701 MS.gene90980:CDS 55.0%
AGCCCGGCCTCAATGGTTAT+GGG + chr4.2:34819588-34819607 MS.gene90980:intron 55.0%
GGTACTCCAAGCACAGGATG+AGG + chr4.2:34818679-34818698 MS.gene90980:CDS 55.0%
GTAGCACAGATGGTTCCCTC+AGG - chr4.2:34820258-34820277 None:intergenic 55.0%
GTTCATCCGCCAAGAAGCTG+AGG + chr4.2:34818052-34818071 MS.gene90980:CDS 55.0%
TAACCATTGAGGCCGGGCTA+TGG - chr4.2:34819587-34819606 None:intergenic 55.0%
TAGCCCGGCCTCAATGGTTA+TGG + chr4.2:34819587-34819606 MS.gene90980:intron 55.0%
TCACCACCTCATCCTGTGCT+TGG - chr4.2:34818688-34818707 None:intergenic 55.0%
GTGCCATAGCCCGGCCTCAA+TGG + chr4.2:34819581-34819600 MS.gene90980:intron 65.0%
TCGCTCCATCGCCAACCACC+CGG + chr4.2:34819704-34819723 MS.gene90980:intron 65.0%
TGAGGCCGGGCTATGGCACA+TGG - chr4.2:34819580-34819599 None:intergenic 65.0%
TTGGTCCGGGTGGTTGGCGA+TGG - chr4.2:34819712-34819731 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 34818008 34821552 34818008 ID=MS.gene90980
chr4.2 mRNA 34818008 34821552 34818008 ID=MS.gene90980.t1;Parent=MS.gene90980
chr4.2 exon 34818008 34818109 34818008 ID=MS.gene90980.t1.exon1;Parent=MS.gene90980.t1
chr4.2 CDS 34818008 34818109 34818008 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
chr4.2 exon 34818382 34818482 34818382 ID=MS.gene90980.t1.exon2;Parent=MS.gene90980.t1
chr4.2 CDS 34818382 34818482 34818382 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
chr4.2 exon 34818643 34818799 34818643 ID=MS.gene90980.t1.exon3;Parent=MS.gene90980.t1
chr4.2 CDS 34818643 34818799 34818643 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
chr4.2 exon 34820244 34820446 34820244 ID=MS.gene90980.t1.exon4;Parent=MS.gene90980.t1
chr4.2 CDS 34820244 34820446 34820244 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
chr4.2 exon 34821257 34821360 34821257 ID=MS.gene90980.t1.exon5;Parent=MS.gene90980.t1
chr4.2 CDS 34821257 34821360 34821257 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
chr4.2 exon 34821473 34821552 34821473 ID=MS.gene90980.t1.exon6;Parent=MS.gene90980.t1
chr4.2 CDS 34821473 34821552 34821473 ID=cds.MS.gene90980.t1;Parent=MS.gene90980.t1
Gene Sequence

>MS.gene90980

ATGAAGGATGCAGATGTCTCAAAGCAAATCCAGCAGATGATTAGGTTCATCCGCCAAGAAGCTGAGGAGAAAGCTAATGAAATATCTGTTGCTGCAGAGGAAGTATGTTTAATTGATAAGTTTCTTTTCATAAAATAATAATTAGACAAGTTAGGTCTTGGTCAGAGACATGCATCGGCAACAAGATTCAAATTCTGGTTCTTATGGGATCTGAGTTGAATCCTTATCAATCGGACCAGCGTTGATTTTTAATTGATAACTTACAAACTTATCATCATTAATGTTATGTTTTGTAAAATGTTTAATAATATGAATATCAATGGATTGGTGATTTCTTTATCTTGTTGAATTTCAATTGTTGTGATTCATTTTAGGAATTCAATATTGAGAAACTACAATTACTTGAAGCTGAAAAAAGGAAGATCAGACAAGAGTACGAGCGAAAGGCCAAACAGATTGATGTTCGTCGAAAAATGTGAGTGAATTAGTACTTCTTTTTTTTTTTTTTTTCAAAAGACTAATTAGTAAAAATTTTAAAGATTAAACTTGCTATAGAAACATTAGAAATTACTACAATTTATTTCAATTCAAAATTAGGAAACCTTTGACAAAACTTTGTTTATTTGCATGAGTAGTGAATACTCGATGCAGCTGAATACATCTCGTATAAAGGTACTCCAAGCACAGGATGAGGTGGTGAGTTCTATGAAAGATGATGCTAAAAAGGCACTTCTACACATCTCAAATGATGAGCAAGTATACAAGAAACTCATCAAAGAAATAATTGTTCAGGTATTTGCGTCATTTTTTTAGACAATGATATCATTTATACATCCAAATTTTATCATTTTCATTAAACACAAATTGCCAACAACAATATAAAAAACACAGTTTTTAATACTTTTTTTTTGAACAAGCAGTTTTTAATACTTAGTTAGGGTTAATTAATTTGTGGAATTGTGTGAATCTTTGACACCTTGTCTGATCGTCACCTTCATTTCTCCTAATTTTTTATAGGGTCTTGCTAACCATTAGTATCCTCAGGGCATATTGGTTAAGGATTTTACAAATAAAAATTTTATTTTGGAAATATATGATGTTACTTTTAACCAAGTAATGATTACATAACTTTTTTATGAAAACTTACTTATTTTGGATGTTTAACGTCCAATACCCTTTGGACATTGGTTAGCATTCACTTTTTTATAAGGTCAATACTTAATTTTAAATAGAACAAAAATAAAATTATTGAATAAATTAGTTGTGTCACTTGCCTCCGATGTAGTATACATAGATCCTTGCATTCTTGGAATGTCCTAATAGTGGGGTTTTTGAAAGGTATTTATGGGTAATGGGTTCAAATCTTGAGTTTGACCAATTTAAAACTGAGTTGAACAAAACATCGTGCCCATGTGTAGTCTGTCTTGCCACTAGAGCATTGTAATTCGTTTGCATAATTATTAGCATTGTTATCCATATGTATAATTGTTAATTTTCAATTTATATAATTGTTCGATTTGTGCCTTATATTATAAATAGAAATATTTTCGGGTCCCAAAAATGTTGATTCCATGTGCCATAGCCCGGCCTCAATGGTTATGGGTTGATGCTGATTGTCAAGTGACCTCTCAAAGCTTCGCATAATTGTTTTTGTTTTTTAAAATGAGTTTATTAATTTTCTACTAAAAGAACGTTTTCGCTCCATCGCCAACCACCCGGACCAAATTGTTTTCAAGCCAACTGCCCAATGCCAACCTTACACCTTTACATATACCAATCAGATACTTCTACCAAGTTGATCCTAACAGTCCATCTTCCCTATTACGATCACCTTCCTTTCCATACCTTGCCACTAAAATGTTATACCATAATCTCTTTTGATCGACATACATCCTCCAACACCATTTACCCAACATCACCATATTTAATTCCCAAATTCTATGAACCCTCGACCCTCCATTCTCCTTATCCAAACAAACAGTATCCAATTAACCCACGAAATTGTGTAAGTGCCCTTAGACTTGTACACTTAATTTTTTTTTTCTACATCCTTTTAATACTCCATTTTCCCTCTATCTCTTTATTCTTATCAAATCACTAATATATCACTTTCATAACATCACTCTCCTTTTCTTTTTCTCCCTAGTGGTAGATTGGTTTTTGGGTGTAAAAAATTATTTTCCAATTGTTAATAATGCTAGCTGAGACAGGGTTAATTTATTGCATGTGTTTGTAGGGTTTACTACGCCTGAGGGAACCATCTGTGCTACTACGATGTAGAGAGGGTGATCGTAAGCTTGTTGAGTCGTTGCTAGAGGATGTCAAAAAAGAACACTCAGAGAAAGCAAATGTGCAACCCCCTAAGATTGCCCTTGATGATCGTGTTTATCTCCCACCTCAACCAAAAAATGACGATGTGGATACGCATGAACCTTTTTGGTATATCCTTCTATTTATCTTTGATTAAATTACCATGAACCCTTTGTACTTTCTAATTGTTTGATTTGTCTAAGTTTGTGTTAAGTTTTATTTAAACCCTTTGTACTTTCTATTGCTTTTTTTATTAGAATCAGATACTTCCATACATCTTAATCGAACAATTATAAATTTAGTCTCTAAATCTAAACTATCATTATGTTCAATTTAGTGGATAAACTATAGAAATATACCAAGTAGTCCTTAACCTATACAAAATCCATCAATTTCAGTCTCTACTATTAAGAAGTAAGGGACTAAATTGACAGATTTTAATTTTAATATACTTTACGGACTAAATTGGTGAAATTTACATAGCTTAGGGGCTGGTTGGTTTATTTATATAGTTTTTTTTTTTTTGGTAGACGTTTATTTATATAGTTTAAGGACGAGTTTATTTCATCTTAATTACTCTTTTTCCGTCCATTATGACATTTTATATTATGACGAAAATTGACAAGTTCTAAAACAATAGATTATTCATTTCCTTGTTTATCATGCCAATTTGTTGTAAAAGTTTACTTATCTTATCACCTTGTTTTCAATAAAAAAAAATGGGATAGATGTATTAGACTAACACAAAAATTTAAGCTTTGATTAAGTTACAAGCTTTGTATGCGTACTTATTCTTTTGTATGAAAACACCTTCAATGCTACTAGAAAATATAAAATTAACGACAAAAATTAGCTGACATACAAAATAAATCTCTCTCTAATTCATGATTAATAAATGATGTACTTTGTGTGTATGCTTATTTATTTATTTCAATGTAGTTCTGGAGGAGTAGTATTGGCCTCAGAGGATGGAAAGATAGTGTTGGAAAATACTCTCGACGCGCGACTTGATGTCATTTTTCGACAAAAGTTGCCCGAGGCATGTTCATTACCTTACACTCATTACATTGATTTCAATCAGAATCTTAAGTATTACCTTAATTGTTAGTGAAAAGGTTAATTATTATTGATTAAAACTTATTTTTGCACTTGCAGATACGAAAGCGTCTGCTAGCTTAAAGTTGAACAAGACGGTGCAATCTGAATTCTATATTGCCACATCTTGTGGAACTTGA
Protein sequence

>MS.gene90980.t1

MKDADVSKQIQQMIRFIRQEAEEKANEISVAAEEEFNIEKLQLLEAEKRKIRQEYERKAKQIDVRRKIEYSMQLNTSRIKVLQAQDEVVSSMKDDAKKALLHISNDEQVYKKLIKEIIVQGLLRLREPSVLLRCREGDRKLVESLLEDVKKEHSEKANVQPPKIALDDRVYLPPQPKNDDVDTHEPFCSGGVVLASEDGKIVLENTLDARLDVIFRQKLPEAYTKASASLKLNKTVQSEFYIATSCGT