Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91526.t1 | XP_003621181.1 | 100 | 89 | 0 | 0 | 1 | 89 | 1 | 89 | 3.10E-42 | 180.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91526.t1 | P40590 | 98.9 | 89 | 1 | 0 | 1 | 89 | 1 | 89 | 6.3e-44 | 177.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91526.t1 | Q2HVS3 | 100.0 | 89 | 0 | 0 | 1 | 89 | 1 | 89 | 2.2e-42 | 180.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050114 | MS.gene91526 | 0.80901 | 2.34E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene91526 | 0.827486 | 1.52E-54 | -1.69E-46 |
| MS.gene051746 | MS.gene91526 | 0.839725 | 1.33E-57 | -1.69E-46 |
| MS.gene051748 | MS.gene91526 | 0.837354 | 5.46E-57 | -1.69E-46 |
| MS.gene051756 | MS.gene91526 | 0.80317 | 3.96E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene91526 | 0.817736 | 2.82E-52 | -1.69E-46 |
| MS.gene053109 | MS.gene91526 | 0.807564 | 4.75E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene91526 | 0.820372 | 7.10E-53 | -1.69E-46 |
| MS.gene057057 | MS.gene91526 | 0.813526 | 2.45E-51 | -1.69E-46 |
| MS.gene057094 | MS.gene91526 | 0.855108 | 7.87E-62 | -1.69E-46 |
| MS.gene058758 | MS.gene91526 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene91526 | 0.806174 | 9.35E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene91526 | 0.807979 | 3.88E-50 | -1.69E-46 |
| MS.gene060463 | MS.gene91526 | 0.843562 | 1.30E-58 | -1.69E-46 |
| MS.gene060465 | MS.gene91526 | 0.853664 | 2.06E-61 | -1.69E-46 |
| MS.gene060702 | MS.gene91526 | 0.832026 | 1.20E-55 | -1.69E-46 |
| MS.gene061055 | MS.gene91526 | 0.809014 | 2.33E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91526.t1 | MTR_5g040570 | 100.000 | 89 | 0 | 0 | 1 | 89 | 1 | 89 | 8.62e-61 | 181 |
| MS.gene91526.t1 | MTR_7g010150 | 100.000 | 89 | 0 | 0 | 1 | 89 | 1 | 89 | 1.06e-60 | 181 |
| MS.gene91526.t1 | MTR_8g467460 | 100.000 | 89 | 0 | 0 | 1 | 89 | 1 | 89 | 1.70e-60 | 180 |
| MS.gene91526.t1 | MTR_7g011050 | 66.292 | 89 | 14 | 1 | 1 | 89 | 1 | 73 | 6.19e-34 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91526.t1 | AT1G26880 | 93.258 | 89 | 6 | 0 | 1 | 89 | 1 | 89 | 9.60e-57 | 171 |
| MS.gene91526.t1 | AT1G69620 | 93.258 | 89 | 6 | 0 | 1 | 89 | 1 | 89 | 2.05e-56 | 170 |
| MS.gene91526.t1 | AT1G26880 | 93.258 | 89 | 6 | 0 | 1 | 89 | 1 | 89 | 4.54e-56 | 168 |
| MS.gene91526.t1 | AT3G28900 | 89.888 | 89 | 9 | 0 | 1 | 89 | 1 | 89 | 4.72e-54 | 164 |
Find 30 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACCGCCGCACTGTTAAC+AGG | 0.305052 | 7.1:+85291410 | MS.gene91526:CDS |
| TGGATCCTCTTTCCAGTAAC+AGG | 0.314412 | 7.1:-85290147 | None:intergenic |
| GGACCTAAGTGTCCTGTTAC+TGG | 0.331250 | 7.1:+85290135 | MS.gene91526:CDS |
| CGCACTGTTAACAGGGCTTA+TGG | 0.356563 | 7.1:+85291418 | MS.gene91526:CDS |
| AACACGATGCTGGTTGGATT+TGG | 0.389836 | 7.1:-85289913 | None:intergenic |
| TTAACAGTGCGGCGGTTCCT+AGG | 0.468395 | 7.1:-85291406 | None:intergenic |
| CCTTTCCCTAACAGCTCCTC+CGG | 0.470814 | 7.1:-85291452 | None:intergenic |
| TTTATCCGGAGGAGCTGTTA+GGG | 0.501294 | 7.1:+85291447 | MS.gene91526:CDS |
| TTTCCAGTAACAGGACACTT+AGG | 0.509665 | 7.1:-85290138 | None:intergenic |
| ACGATGCTGGTTGGATTTGG+TGG | 0.532546 | 7.1:-85289910 | None:intergenic |
| CATCGTGTTGTTAAGACACC+TGG | 0.536949 | 7.1:+85289927 | MS.gene91526:CDS |
| AGTGTCCTGTTACTGGAAAG+AGG | 0.543803 | 7.1:+85290142 | MS.gene91526:CDS |
| CTTAACAACACGATGCTGGT+TGG | 0.545517 | 7.1:-85289919 | None:intergenic |
| GTTACTGGAAAGAGGATCCA+AGG | 0.571456 | 7.1:+85290150 | MS.gene91526:CDS |
| ACAACTAAGAAGAGAGCTAG+TGG | 0.577057 | 7.1:+85290114 | MS.gene91526:CDS |
| GTGTCTTAACAACACGATGC+TGG | 0.578379 | 7.1:-85289923 | None:intergenic |
| TGGCATAGCTGTGTCTCCTA+CGG | 0.580308 | 7.1:-85289890 | None:intergenic |
| CCGGAGGAGCTGTTAGGGAA+AGG | 0.595594 | 7.1:+85291452 | MS.gene91526:CDS |
| GAACCGCCGCACTGTTAACA+GGG | 0.611783 | 7.1:+85291411 | MS.gene91526:CDS |
| CTAGACCTCTTGTATTCAGT+AGG | 0.617053 | 7.1:-85291379 | None:intergenic |
| TTACTGGAAAGAGGATCCAA+GGG | 0.629653 | 7.1:+85290151 | MS.gene91526:CDS |
| TGAGACCTACTGAATACAAG+AGG | 0.631121 | 7.1:+85291374 | MS.gene91526:CDS |
| ACAAGAGGTCTAGATTGCCT+AGG | 0.636078 | 7.1:+85291389 | MS.gene91526:CDS |
| TATTCAGTAGGTCTCAAGTG+TGG | 0.667813 | 7.1:-85291367 | None:intergenic |
| AAGCCCTGTTAACAGTGCGG+CGG | 0.691394 | 7.1:-85291414 | None:intergenic |
| ACTGTTAACAGGGCTTATGG+AGG | 0.693206 | 7.1:+85291421 | MS.gene91526:CDS |
| TTCAGAGACTCACATACCGT+AGG | 0.721913 | 7.1:+85289874 | MS.gene91526:CDS |
| CATAAGCCCTGTTAACAGTG+CGG | 0.730145 | 7.1:-85291417 | None:intergenic |
| TACTGGAAAGAGGATCCAAG+GGG | 0.738216 | 7.1:+85290152 | MS.gene91526:CDS |
| GTATGTGAGTCTCTGAACCA+TGG | 0.739024 | 7.1:-85289868 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATAAAATCAATTATTTG+TGG | + | chr7.1:85290438-85290457 | MS.gene91526:intron | 10.0% |
| !! | ATTTGAAAATTGAAAATATT+AGG | - | chr7.1:85290522-85290541 | None:intergenic | 10.0% |
| !!! | TTTTTTAGAAATTAATATGT+TGG | + | chr7.1:85290196-85290215 | MS.gene91526:intron | 10.0% |
| !! | TAACAAATCTTCAATAAAAA+TGG | - | chr7.1:85290852-85290871 | None:intergenic | 15.0% |
| !! | TTTGCAATTATTAATGTTAA+TGG | + | chr7.1:85290403-85290422 | MS.gene91526:intron | 15.0% |
| !!! | TTTGATTAATTTGTGAAAAT+GGG | + | chr7.1:85290056-85290075 | MS.gene91526:intron | 15.0% |
| !!! | TTTTGATTAATTTGTGAAAA+TGG | + | chr7.1:85290055-85290074 | MS.gene91526:intron | 15.0% |
| !! | GAATGAAAATTAAAAGTAGT+AGG | - | chr7.1:85291226-85291245 | None:intergenic | 20.0% |
| !!! | TTAATACTACAATTTTGTGA+GGG | - | chr7.1:85291012-85291031 | None:intergenic | 20.0% |
| !!! | TTAATATGTTGGTTATACAT+AGG | + | chr7.1:85290207-85290226 | MS.gene91526:intron | 20.0% |
| !!! | TTTAATACTACAATTTTGTG+AGG | - | chr7.1:85291013-85291032 | None:intergenic | 20.0% |
| ! | AAACTATCTCTTCTGTAATA+GGG | - | chr7.1:85290710-85290729 | None:intergenic | 25.0% |
| ! | AATGTTCTTACTTCAATGTT+GGG | + | chr7.1:85290472-85290491 | MS.gene91526:intron | 25.0% |
| ! | AGATACCCTTTCTAAATTAT+TGG | + | chr7.1:85291032-85291051 | MS.gene91526:intron | 25.0% |
| ! | AGTCACCAATAATTTAGAAA+GGG | - | chr7.1:85291040-85291059 | None:intergenic | 25.0% |
| ! | ATCTATCATGTTTCAAACAT+AGG | - | chr7.1:85290876-85290895 | None:intergenic | 25.0% |
| ! | GAAAATTAAAAGTAGTAGGT+AGG | - | chr7.1:85291222-85291241 | None:intergenic | 25.0% |
| ! | GACTTGTATTATTCATCAAT+TGG | + | chr7.1:85290323-85290342 | MS.gene91526:intron | 25.0% |
| ! | GATATATGTGAGTTTATGAT+AGG | + | chr7.1:85290301-85290320 | MS.gene91526:intron | 25.0% |
| ! | GCATCATTGAAATTATACAA+TGG | - | chr7.1:85291125-85291144 | None:intergenic | 25.0% |
| ! | TGTAAAATAGGTGGAATATT+AGG | + | chr7.1:85290368-85290387 | MS.gene91526:intron | 25.0% |
| ! | TTATCATATTTGTTCTGTTC+TGG | + | chr7.1:85291328-85291347 | MS.gene91526:intron | 25.0% |
| ! | TTGAATTCATCTATGTTAGT+TGG | - | chr7.1:85290552-85290571 | None:intergenic | 25.0% |
| ! | TTGTCTGTGTTATGTAAAAT+AGG | + | chr7.1:85290356-85290375 | MS.gene91526:intron | 25.0% |
| AAGTGTCAAGATTATATGCA+GGG | - | chr7.1:85291198-85291217 | None:intergenic | 30.0% | |
| CAAACTATCTCTTCTGTAAT+AGG | - | chr7.1:85290711-85290730 | None:intergenic | 30.0% | |
| CAACACAATATTCAATCAGA+TGG | - | chr7.1:85290680-85290699 | None:intergenic | 30.0% | |
| CAGTCACCAATAATTTAGAA+AGG | - | chr7.1:85291041-85291060 | None:intergenic | 30.0% | |
| GAATGTTCTTACTTCAATGT+TGG | + | chr7.1:85290471-85290490 | MS.gene91526:intron | 30.0% | |
| TCTGTGTTATGTAAAATAGG+TGG | + | chr7.1:85290359-85290378 | MS.gene91526:intron | 30.0% | |
| ! | AAAATTGATGATAGCGTCAA+AGG | - | chr7.1:85290922-85290941 | None:intergenic | 30.0% |
| ! | AAAGTGTCAAGATTATATGC+AGG | - | chr7.1:85291199-85291218 | None:intergenic | 30.0% |
| !!! | AAATGGGTTTGTGTTGTTTT+AGG | + | chr7.1:85290072-85290091 | MS.gene91526:intron | 30.0% |
| CAATTCTACAACATTGTGGA+AGG | + | chr7.1:85291144-85291163 | MS.gene91526:intron | 35.0% | |
| GATGCAATTCTACAACATTG+TGG | + | chr7.1:85291140-85291159 | MS.gene91526:intron | 35.0% | |
| TCATATAGATTCATACCCCT+TGG | - | chr7.1:85290170-85290189 | None:intergenic | 35.0% | |
| TGACAAAGAAGAACATTACC+AGG | - | chr7.1:85289948-85289967 | None:intergenic | 35.0% | |
| TTTGTTTGTACATGATGCTG+TGG | + | chr7.1:85290265-85290284 | MS.gene91526:intron | 35.0% | |
| ! | CAATTTTGTGAGGGACAATA+AGG | - | chr7.1:85291003-85291022 | None:intergenic | 35.0% |
| !!! | TGTTGTTTTAGGTGGAAAGT+TGG | + | chr7.1:85290083-85290102 | MS.gene91526:intron | 35.0% |
| AAACTTAGACGCTGTGTGAT+AGG | - | chr7.1:85291099-85291118 | None:intergenic | 40.0% | |
| ACAACTAAGAAGAGAGCTAG+TGG | + | chr7.1:85290114-85290133 | MS.gene91526:CDS | 40.0% | |
| ATAGCGTCAAAGGTCATAGT+TGG | - | chr7.1:85290912-85290931 | None:intergenic | 40.0% | |
| CTAGACCTCTTGTATTCAGT+AGG | - | chr7.1:85291382-85291401 | None:intergenic | 40.0% | |
| CTTCACAAACACAGCTGAAT+TGG | - | chr7.1:85290793-85290812 | None:intergenic | 40.0% | |
| TATTCAGTAGGTCTCAAGTG+TGG | - | chr7.1:85291370-85291389 | None:intergenic | 40.0% | |
| TGAGACCTACTGAATACAAG+AGG | + | chr7.1:85291374-85291393 | MS.gene91526:CDS | 40.0% | |
| TTACTGGAAAGAGGATCCAA+GGG | + | chr7.1:85290151-85290170 | MS.gene91526:CDS | 40.0% | |
| TTTCCAGTAACAGGACACTT+AGG | - | chr7.1:85290141-85290160 | None:intergenic | 40.0% | |
| TTTGTGAGGGACAATAAGGA+CGG | - | chr7.1:85290999-85291018 | None:intergenic | 40.0% | |
| !!! | GCTTATGGAGGTGTTTTATC+CGG | + | chr7.1:85291433-85291452 | MS.gene91526:CDS | 40.0% |
| !!! | TCATTGCTTTTTCTCCCTGA+TGG | + | chr7.1:85291253-85291272 | MS.gene91526:intron | 40.0% |
| !!! | TGGGTTTGTGTTGTTTTAGG+TGG | + | chr7.1:85290075-85290094 | MS.gene91526:intron | 40.0% |
| ACAAGAGGTCTAGATTGCCT+AGG | + | chr7.1:85291389-85291408 | MS.gene91526:CDS | 45.0% | |
| ACTGTTAACAGGGCTTATGG+AGG | + | chr7.1:85291421-85291440 | MS.gene91526:CDS | 45.0% | |
| AGTGCATGTCATGACCATCA+GGG | - | chr7.1:85291270-85291289 | None:intergenic | 45.0% | |
| AGTGTCCTGTTACTGGAAAG+AGG | + | chr7.1:85290142-85290161 | MS.gene91526:CDS | 45.0% | |
| CATAAGCCCTGTTAACAGTG+CGG | - | chr7.1:85291420-85291439 | None:intergenic | 45.0% | |
| CTTAACAACACGATGCTGGT+TGG | - | chr7.1:85289922-85289941 | None:intergenic | 45.0% | |
| GTGTCTTAACAACACGATGC+TGG | - | chr7.1:85289926-85289945 | None:intergenic | 45.0% | |
| GTTACTGGAAAGAGGATCCA+AGG | + | chr7.1:85290150-85290169 | MS.gene91526:CDS | 45.0% | |
| TACTGGAAAGAGGATCCAAG+GGG | + | chr7.1:85290152-85290171 | MS.gene91526:CDS | 45.0% | |
| TGGATCCTCTTTCCAGTAAC+AGG | - | chr7.1:85290150-85290169 | None:intergenic | 45.0% | |
| TTCAGAGACTCACATACCGT+AGG | + | chr7.1:85289874-85289893 | MS.gene91526:CDS | 45.0% | |
| TTTATCCGGAGGAGCTGTTA+GGG | + | chr7.1:85291447-85291466 | MS.gene91526:CDS | 45.0% | |
| ! | AACACGATGCTGGTTGGATT+TGG | - | chr7.1:85289916-85289935 | None:intergenic | 45.0% |
| ! | CATCGTGTTGTTAAGACACC+TGG | + | chr7.1:85289927-85289946 | MS.gene91526:CDS | 45.0% |
| ! | TTTTATCCGGAGGAGCTGTT+AGG | + | chr7.1:85291446-85291465 | MS.gene91526:CDS | 45.0% |
| !!! | TATGGAGGTGTTTTATCCGG+AGG | + | chr7.1:85291436-85291455 | MS.gene91526:CDS | 45.0% |
| CGCACTGTTAACAGGGCTTA+TGG | + | chr7.1:85291418-85291437 | MS.gene91526:CDS | 50.0% | |
| GAGTGCATGTCATGACCATC+AGG | - | chr7.1:85291271-85291290 | None:intergenic | 50.0% | |
| GGACCTAAGTGTCCTGTTAC+TGG | + | chr7.1:85290135-85290154 | MS.gene91526:CDS | 50.0% | |
| GTCTCAAGTGTGGAATCTGC+AGG | - | chr7.1:85291360-85291379 | None:intergenic | 50.0% | |
| TGGCATAGCTGTGTCTCCTA+CGG | - | chr7.1:85289893-85289912 | None:intergenic | 50.0% | |
| ! | ACGATGCTGGTTGGATTTGG+TGG | - | chr7.1:85289913-85289932 | None:intergenic | 50.0% |
| AAGCCCTGTTAACAGTGCGG+CGG | - | chr7.1:85291417-85291436 | None:intergenic | 55.0% | |
| GAACCGCCGCACTGTTAACA+GGG | + | chr7.1:85291411-85291430 | MS.gene91526:CDS | 55.0% | |
| TTAACAGTGCGGCGGTTCCT+AGG | - | chr7.1:85291409-85291428 | None:intergenic | 55.0% | |
| GGAACCGCCGCACTGTTAAC+AGG | + | chr7.1:85291410-85291429 | MS.gene91526:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 85289870 | 85291473 | 85289870 | ID=MS.gene91526 |
| chr7.1 | mRNA | 85289870 | 85291473 | 85289870 | ID=MS.gene91526.t1;Parent=MS.gene91526 |
| chr7.1 | exon | 85289870 | 85289948 | 85289870 | ID=MS.gene91526.t1.exon1;Parent=MS.gene91526.t1 |
| chr7.1 | CDS | 85289870 | 85289948 | 85289870 | ID=cds.MS.gene91526.t1;Parent=MS.gene91526.t1 |
| chr7.1 | exon | 85290094 | 85290173 | 85290094 | ID=MS.gene91526.t1.exon2;Parent=MS.gene91526.t1 |
| chr7.1 | CDS | 85290094 | 85290173 | 85290094 | ID=cds.MS.gene91526.t1;Parent=MS.gene91526.t1 |
| chr7.1 | exon | 85291364 | 85291473 | 85291364 | ID=MS.gene91526.t1.exon3;Parent=MS.gene91526.t1 |
| chr7.1 | CDS | 85291364 | 85291473 | 85291364 | ID=cds.MS.gene91526.t1;Parent=MS.gene91526.t1 |
| Gene Sequence |
| Protein sequence |
