Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061055.t1 | XP_013466710.1 | 89.4 | 508 | 54 | 0 | 1 | 508 | 1 | 508 | 1.60E-220 | 775.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061055.t1 | A0A072VH38 | 89.4 | 508 | 54 | 0 | 1 | 508 | 1 | 508 | 1.2e-220 | 775.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060702 | MS.gene061055 | 0.836382 | 9.67E-57 | -1.69E-46 |
| MS.gene061055 | MS.gene07608 | 0.802821 | 4.68E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene26426 | 0.808061 | 3.73E-50 | -1.69E-46 |
| MS.gene061055 | MS.gene28159 | 0.818139 | 2.29E-52 | -1.69E-46 |
| MS.gene061055 | MS.gene44260 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene061055 | MS.gene54555 | 0.801459 | 8.92E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene55530 | 0.838836 | 2.27E-57 | -1.69E-46 |
| MS.gene061055 | MS.gene70305 | 0.836567 | 8.68E-57 | -1.69E-46 |
| MS.gene061055 | MS.gene70530 | 0.80353 | 3.34E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene75884 | 0.803693 | 3.09E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene76478 | 0.808958 | 2.40E-50 | -1.69E-46 |
| MS.gene061055 | MS.gene87972 | 0.802748 | 4.84E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene91526 | 0.809014 | 2.33E-50 | -1.69E-46 |
| MS.gene061055 | MS.gene97690 | 0.803142 | 4.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061055.t1 | MTR_1g034170 | 96.654 | 508 | 17 | 0 | 1 | 508 | 1 | 508 | 0.0 | 994 |
| MS.gene061055.t1 | MTR_1g034170 | 96.338 | 355 | 13 | 0 | 154 | 508 | 2 | 356 | 0.0 | 703 |
| MS.gene061055.t1 | MTR_1g034170 | 96.215 | 317 | 12 | 0 | 192 | 508 | 1 | 317 | 0.0 | 628 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061055.t1 | AT2G15860 | 62.815 | 476 | 157 | 8 | 36 | 508 | 54 | 512 | 0.0 | 545 |
| MS.gene061055.t1 | AT2G15860 | 61.270 | 488 | 157 | 9 | 36 | 508 | 54 | 524 | 0.0 | 535 |
| MS.gene061055.t1 | AT2G15860 | 61.848 | 422 | 130 | 8 | 36 | 443 | 54 | 458 | 7.87e-159 | 459 |
| MS.gene061055.t1 | AT2G15860 | 62.019 | 416 | 127 | 8 | 36 | 437 | 54 | 452 | 8.43e-158 | 456 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGCCGGTGGCGGCGGAAAT+TGG | 0.125991 | 1.4:+18774574 | MS.gene061055:CDS |
| CTCAGCAGTAACACCTTTAA+TGG | 0.213532 | 1.4:-18781703 | None:intergenic |
| AAAATCTGCTCTAGATAAAT+TGG | 0.241893 | 1.4:+18775599 | MS.gene061055:CDS |
| ATCTTGGACTGATGCCTTCT+TGG | 0.262105 | 1.4:-18781742 | None:intergenic |
| CGCCGGTGGCGGCGGAAATT+GGG | 0.295480 | 1.4:+18774575 | MS.gene061055:CDS |
| GCCTATCAAGCAGCCATTAA+AGG | 0.303168 | 1.4:+18781690 | MS.gene061055:CDS |
| TCCGGAGCATGGAGTGCTTT+AGG | 0.305948 | 1.4:+18776240 | MS.gene061055:CDS |
| CGGCGGAAATTGGGGAGGTT+GGG | 0.309579 | 1.4:+18774584 | MS.gene061055:CDS |
| ACCTTTAATGGCTGCTTGAT+AGG | 0.312993 | 1.4:-18781691 | None:intergenic |
| TAATGCACCTTCCCTATTAG+AGG | 0.313353 | 1.4:+18777115 | MS.gene061055:CDS |
| CTAAATATCCAATCATTGTC+TGG | 0.353735 | 1.4:-18780464 | None:intergenic |
| GCGGCGGAAATTGGGGAGGT+TGG | 0.359235 | 1.4:+18774583 | MS.gene061055:CDS |
| GACTTATTAATCAGTGAAAC+TGG | 0.359424 | 1.4:+18777289 | MS.gene061055:CDS |
| GCATTAGAACCTGGAGTTCC+TGG | 0.359883 | 1.4:-18777098 | None:intergenic |
| AGAAAGCACTGGCTTTATCT+TGG | 0.360530 | 1.4:-18781758 | None:intergenic |
| TCTTGGACTGATGCCTTCTT+GGG | 0.360629 | 1.4:-18781741 | None:intergenic |
| ATAATTGATCTTCATCGCTT+TGG | 0.367189 | 1.4:-18777348 | None:intergenic |
| GGCGGAAATTGGGGAGGTTG+GGG | 0.372900 | 1.4:+18774585 | MS.gene061055:CDS |
| GTTTCACAACTTCTAACGTT+TGG | 0.392699 | 1.4:+18780324 | MS.gene061055:CDS |
| GATATAATTCAGAGAACTAC+TGG | 0.394582 | 1.4:+18780112 | MS.gene061055:CDS |
| ATAAATGCCCAGACAATGAT+TGG | 0.403832 | 1.4:+18780456 | MS.gene061055:CDS |
| AAAGTTACCTCCAGCTGCTC+TGG | 0.404860 | 1.4:-18777415 | None:intergenic |
| AGTTTCACAAATGCCTTGGC+TGG | 0.417316 | 1.4:+18780076 | MS.gene061055:intron |
| AAGGCTGCTGAAATGGCTGC+TGG | 0.417716 | 1.4:+18779628 | MS.gene061055:CDS |
| AAGTTGTTCATTGTCGTTGT+TGG | 0.419492 | 1.4:-18774532 | None:intergenic |
| TGTTGGCAAGGCTGCTGAAA+TGG | 0.423476 | 1.4:+18779621 | MS.gene061055:CDS |
| GTCTTCTAAAGAGGATTTAG+TGG | 0.434315 | 1.4:+18775524 | MS.gene061055:CDS |
| GTCATCAACTTCTTCATCCT+CGG | 0.436498 | 1.4:-18780376 | None:intergenic |
| ATTGAAAAGGCTAGAAAAGA+AGG | 0.439240 | 1.4:+18777319 | MS.gene061055:CDS |
| GTAATGTGTGTAGGGTTTGA+AGG | 0.448752 | 1.4:+18776188 | MS.gene061055:intron |
| TTTGTTGGCAAGAGAAATGA+TGG | 0.452056 | 1.4:-18780352 | None:intergenic |
| AGTGGATGAAGAAGCTGAAA+AGG | 0.457687 | 1.4:+18775542 | MS.gene061055:CDS |
| TTATGGAGGTCCAGAGCAGC+TGG | 0.460533 | 1.4:+18777405 | MS.gene061055:CDS |
| GAGAATTCAGCTTCAAACCT+TGG | 0.461518 | 1.4:+18777053 | MS.gene061055:CDS |
| TCCCCAATTTCCGCCGCCAC+CGG | 0.462650 | 1.4:-18774577 | None:intergenic |
| ACACAAGACGCCGGTGGCGG+CGG | 0.486559 | 1.4:+18774567 | MS.gene061055:CDS |
| TTTCAGTTTCACAAATGCCT+TGG | 0.493240 | 1.4:+18780072 | MS.gene061055:intron |
| TCCTAAAGCACTCCATGCTC+CGG | 0.494229 | 1.4:-18776241 | None:intergenic |
| CACAGCTATAAGTAAAATCC+AGG | 0.500617 | 1.4:+18781806 | MS.gene061055:CDS |
| TGAAGAAGTTGATGACGATA+AGG | 0.503369 | 1.4:+18780383 | MS.gene061055:CDS |
| GCCGGTGGCGGCGGAAATTG+GGG | 0.510254 | 1.4:+18774576 | MS.gene061055:CDS |
| TGAAGATTCACTACTTAGCC+AGG | 0.511588 | 1.4:+18775632 | MS.gene061055:CDS |
| ACAATAAAGAAAGGAAATGC+AGG | 0.517761 | 1.4:+18779562 | MS.gene061055:CDS |
| GCCTTCTTGGGAACAACTGT+GGG | 0.520547 | 1.4:-18781729 | None:intergenic |
| GCAACACAAGACGCCGGTGG+CGG | 0.526130 | 1.4:+18774564 | MS.gene061055:CDS |
| GCTATAAGTAAAATCCAGGA+TGG | 0.526975 | 1.4:+18781810 | MS.gene061055:CDS |
| AATTTACATGATGCAAGTGT+TGG | 0.529843 | 1.4:+18779604 | MS.gene061055:CDS |
| AGCAAAAGTCTGCATTTGAC+GGG | 0.535286 | 1.4:+18779464 | MS.gene061055:CDS |
| GCAAATCGCTGCTGAAGACT+CGG | 0.539802 | 1.4:+18775500 | MS.gene061055:CDS |
| TAAATATCCAATCATTGTCT+GGG | 0.539887 | 1.4:-18780463 | None:intergenic |
| CATGATGGACCAGGAACTCC+AGG | 0.540249 | 1.4:+18777089 | MS.gene061055:CDS |
| TTAGACTCTCTTCATTCTGA+GGG | 0.540731 | 1.4:+18780139 | MS.gene061055:CDS |
| TCCCACAGTTGTTCCCAAGA+AGG | 0.544027 | 1.4:+18781728 | MS.gene061055:CDS |
| AACTGGAATTGAGATTGAAA+AGG | 0.545630 | 1.4:+18777306 | MS.gene061055:CDS |
| GAATTATATCATTAGCAGCT+AGG | 0.548106 | 1.4:-18780099 | None:intergenic |
| GGAGGATCTGTACCGCATGA+TGG | 0.549991 | 1.4:+18777074 | MS.gene061055:CDS |
| CTATAAGTAAAATCCAGGAT+GGG | 0.550409 | 1.4:+18781811 | MS.gene061055:CDS |
| AATTCAGCTTCAAACCTTGG+AGG | 0.553437 | 1.4:+18777056 | MS.gene061055:CDS |
| ATTAGACTCTCTTCATTCTG+AGG | 0.553694 | 1.4:+18780138 | MS.gene061055:CDS |
| TTGAGTAATGAAATTGATGC+TGG | 0.556947 | 1.4:+18779517 | MS.gene061055:CDS |
| AATGCAAGTGCTTGAATATG+TGG | 0.559060 | 1.4:+18777252 | MS.gene061055:CDS |
| AGGCTGCTGAAATGGCTGCT+GGG | 0.562196 | 1.4:+18779629 | MS.gene061055:CDS |
| GAGCAAAAGTCTGCATTTGA+CGG | 0.562921 | 1.4:+18779463 | MS.gene061055:CDS |
| GATCTCTTCGGCGGCAACAG+CGG | 0.575145 | 1.4:-18774643 | None:intergenic |
| ATTTGACGGGAAGCTTAAAG+AGG | 0.577894 | 1.4:+18779477 | MS.gene061055:CDS |
| TGCCTTCTTGGGAACAACTG+TGG | 0.578909 | 1.4:-18781730 | None:intergenic |
| TTCCGAACCTCTAATAGGGA+AGG | 0.580394 | 1.4:-18777122 | None:intergenic |
| GCTGAAAAGGAAAGCGACGA+TGG | 0.580957 | 1.4:+18775555 | MS.gene061055:CDS |
| AGTGCTTGAATATGTGGGTA+AGG | 0.584715 | 1.4:+18777258 | MS.gene061055:CDS |
| CTGAAAAGGAAAGCGACGAT+GGG | 0.592950 | 1.4:+18775556 | MS.gene061055:CDS |
| AAGAAAGGAAATGCAGGAAG+CGG | 0.592995 | 1.4:+18779568 | MS.gene061055:CDS |
| TCTGTACCGCATGATGGACC+AGG | 0.594388 | 1.4:+18777080 | MS.gene061055:CDS |
| AGGGAAGGTGCATTAGAACC+TGG | 0.595416 | 1.4:-18777107 | None:intergenic |
| CCAACCTGTAGTAAAACTGT+TGG | 0.597807 | 1.4:-18780496 | None:intergenic |
| ATTAGCAGCTAGGCCAGCCA+AGG | 0.601827 | 1.4:-18780089 | None:intergenic |
| AGACTCGGAGTCTTCTAAAG+AGG | 0.607550 | 1.4:+18775515 | MS.gene061055:CDS |
| TTAGGAAATGCATGGAGAGG+TGG | 0.610161 | 1.4:+18776258 | MS.gene061055:CDS |
| TACATTGCGAGAGATCTCTT+CGG | 0.611653 | 1.4:-18774655 | None:intergenic |
| CTGTGATTTGATCAGCTCGA+AGG | 0.617243 | 1.4:-18781788 | None:intergenic |
| TATAAGTAAAATCCAGGATG+GGG | 0.618547 | 1.4:+18781812 | MS.gene061055:CDS |
| CAGCATTCATCGAGGTCGAG+AGG | 0.620130 | 1.4:-18781857 | None:intergenic |
| GGAGTTCCTGGTCCATCATG+CGG | 0.627103 | 1.4:-18777086 | None:intergenic |
| GCTGGTGCTGATTCTAACAA+AGG | 0.630570 | 1.4:+18779535 | MS.gene061055:CDS |
| ACGATAAGGCTAACATAGAG+TGG | 0.631053 | 1.4:+18780397 | MS.gene061055:CDS |
| GGTGGCGGCGGAAATTGGGG+AGG | 0.633072 | 1.4:+18774579 | MS.gene061055:CDS |
| ACATGATGCAAGTGTTGGCA+AGG | 0.633721 | 1.4:+18779609 | MS.gene061055:CDS |
| ATGCAATTCTCAGGAACTGG+AGG | 0.646518 | 1.4:+18779384 | MS.gene061055:intron |
| GACAGCATCAAAGAGCATTG+CGG | 0.653694 | 1.4:+18775473 | MS.gene061055:CDS |
| TTCTCTTGCCAACAAAACCG+AGG | 0.657069 | 1.4:+18780359 | MS.gene061055:CDS |
| CATGCGGTACAGATCCTCCA+AGG | 0.659167 | 1.4:-18777070 | None:intergenic |
| AGGCATATCTGATGTAACTG+AGG | 0.666646 | 1.4:+18781668 | MS.gene061055:intron |
| ATGCAAGTGCTTGAATATGT+GGG | 0.667279 | 1.4:+18777253 | MS.gene061055:CDS |
| AAGGTGTTCAGAGAAAGCAC+TGG | 0.669001 | 1.4:-18781769 | None:intergenic |
| GGAGTGCTTTAGGAAATGCA+TGG | 0.670171 | 1.4:+18776250 | MS.gene061055:CDS |
| GCTTTAGGAAATGCATGGAG+AGG | 0.672523 | 1.4:+18776255 | MS.gene061055:CDS |
| CAAGCAACACAAGACGCCGG+TGG | 0.681220 | 1.4:+18774561 | MS.gene061055:CDS |
| ATATGTGGGTAAGGAGACGA+TGG | 0.687721 | 1.4:+18777267 | MS.gene061055:CDS |
| TGGAGGTCCAGAGCAGCTGG+AGG | 0.689393 | 1.4:+18777408 | MS.gene061055:CDS |
| GAACAAGCAACACAAGACGC+CGG | 0.707222 | 1.4:+18774558 | MS.gene061055:CDS |
| ATTGCGAGAGATCTCTTCGG+CGG | 0.716928 | 1.4:-18774652 | None:intergenic |
| TATTCAAGCAGCATTCATCG+AGG | 0.745491 | 1.4:-18781865 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 18774513 | 18781884 | 18774513 | ID=MS.gene061055 |
| chr1.4 | mRNA | 18774513 | 18781884 | 18774513 | ID=MS.gene061055.t1;Parent=MS.gene061055 |
| chr1.4 | exon | 18774513 | 18774674 | 18774513 | ID=MS.gene061055.t1.exon1;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18774513 | 18774674 | 18774513 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18775456 | 18775653 | 18775456 | ID=MS.gene061055.t1.exon2;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18775456 | 18775653 | 18775456 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18776201 | 18776298 | 18776201 | ID=MS.gene061055.t1.exon3;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18776201 | 18776298 | 18776201 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18777049 | 18777136 | 18777049 | ID=MS.gene061055.t1.exon4;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18777049 | 18777136 | 18777049 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18777226 | 18777429 | 18777226 | ID=MS.gene061055.t1.exon5;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18777226 | 18777429 | 18777226 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18779397 | 18779650 | 18779397 | ID=MS.gene061055.t1.exon6;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18779397 | 18779650 | 18779397 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18780078 | 18780168 | 18780078 | ID=MS.gene061055.t1.exon7;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18780078 | 18780168 | 18780078 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18780297 | 18780513 | 18780297 | ID=MS.gene061055.t1.exon8;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18780297 | 18780513 | 18780297 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| chr1.4 | exon | 18781670 | 18781884 | 18781670 | ID=MS.gene061055.t1.exon9;Parent=MS.gene061055.t1 |
| chr1.4 | CDS | 18781670 | 18781884 | 18781670 | ID=cds.MS.gene061055.t1;Parent=MS.gene061055.t1 |
| Gene Sequence |
| Protein sequence |
